FastQCFastQC Report
Thu 26 May 2016
SRR936636_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936636_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754684
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48680.645038188168823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39570.524325413020549No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23000.30476331815700347No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11730.15542929226007177No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8230.10905226558400602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36600.033.766991
GTATCAA58100.033.7070851
TAACGCA700.0083609427.162024
TATCAAC71600.027.0861472
ATCAACG71150.027.0570353
TACCTGG7950.026.9057732
TCAACGC71750.026.7645264
CAACGCA72400.026.5916565
AACGCAG74000.026.080946
GTACATG45950.025.9649981
GTACCTG9050.025.7363871
TACATGG46250.024.9743772
ACATGGG46200.024.3840873
ACGCAGA81600.023.535327
GAGTACT42700.023.49470112-13
CGCAGAG82100.023.4561128
GTATAGG2252.7866918E-923.2386171
CATGGGA27750.022.4392554
GCAGAGT85200.022.2707639
CATTCCG1503.0041578E-522.1926219
Produced by FastQC (version 0.11.5)