##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936636_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 754684 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.345670505801102 31.0 31.0 34.0 30.0 34.0 2 31.389143005549343 31.0 31.0 34.0 28.0 34.0 3 31.304963401900665 31.0 31.0 34.0 28.0 34.0 4 34.99282481144426 37.0 35.0 37.0 32.0 37.0 5 35.03557647969216 37.0 35.0 37.0 32.0 37.0 6 35.10074680263528 37.0 35.0 37.0 32.0 37.0 7 34.96576712902354 37.0 35.0 37.0 32.0 37.0 8 34.95358587170259 37.0 35.0 37.0 32.0 37.0 9 36.43462031790789 39.0 37.0 39.0 32.0 39.0 10-11 36.319012328338744 39.0 37.0 39.0 32.0 39.0 12-13 36.20681901299087 39.0 35.0 39.0 32.0 39.0 14-15 37.16992343815424 39.5 36.5 41.0 31.0 41.0 16-17 37.09524251209778 39.0 36.0 41.0 31.0 41.0 18-19 37.020307174923545 39.0 36.0 41.0 31.0 41.0 20-21 37.05248024338664 39.0 36.0 41.0 31.0 41.0 22-23 37.03964838263432 39.0 36.0 41.0 31.0 41.0 24-25 37.1489047071357 39.0 36.0 41.0 31.0 41.0 26-27 37.06879766895813 39.0 36.0 41.0 31.0 41.0 28-29 36.95346529143323 39.0 36.0 41.0 30.5 41.0 30-31 36.715717439352105 39.0 36.0 41.0 30.0 41.0 32-33 36.50230891339952 39.0 35.0 41.0 30.0 41.0 34-35 36.29941869709707 39.0 35.0 40.5 30.0 41.0 36-37 36.07319089844226 38.5 35.0 40.0 29.5 41.0 38-39 35.87861939566759 38.0 35.0 40.0 29.0 41.0 40-41 35.63315771899232 38.0 35.0 40.0 27.5 41.0 42-43 35.31676900530553 38.0 34.5 40.0 26.5 41.0 44-45 35.05683305860466 38.0 34.0 40.0 25.5 41.0 46-47 34.787038681090365 38.0 34.0 40.0 24.0 41.0 48-49 34.47975311521114 38.0 34.0 40.0 23.0 41.0 50-51 33.457316572234205 37.5 32.5 39.5 21.5 40.5 52-53 33.55268562736191 37.5 33.0 39.5 21.5 40.5 54-55 33.99076302134404 38.0 33.5 40.0 21.5 41.0 56-57 33.84759912758187 38.0 33.0 40.0 20.0 41.0 58-59 33.631492121205696 38.0 33.0 40.0 19.5 41.0 60-61 33.36099678805964 37.0 33.0 40.0 18.5 41.0 62-63 33.02246436919293 37.0 32.5 40.0 17.0 41.0 64-65 32.66416473649899 36.0 32.0 39.5 13.5 41.0 66-67 32.31067373894239 36.0 31.5 39.0 12.5 41.0 68-69 31.900749187739503 35.0 31.0 39.0 8.0 40.5 70-71 31.503060221231667 35.0 31.0 38.5 7.5 40.0 72-73 31.09957280133142 35.0 31.0 37.5 6.5 40.0 74-75 30.684629593313225 35.0 30.0 37.0 2.0 39.0 76-77 30.235368710612654 35.0 30.0 36.5 2.0 39.0 78-79 29.76773855017464 34.0 29.0 36.0 2.0 38.5 80-81 29.326481017220452 34.0 29.0 35.5 2.0 37.0 82-83 28.940116658097956 34.0 29.0 35.0 2.0 37.0 84-85 28.59009598719464 34.0 29.0 35.0 2.0 36.5 86-87 28.243503241091634 34.0 28.0 35.0 2.0 36.0 88-89 27.89697608535493 34.0 27.0 35.0 2.0 36.0 90-91 27.551515601231774 34.0 26.5 35.0 2.0 35.0 92-93 27.139302012497943 33.5 26.0 35.0 2.0 35.0 94-95 26.67825275214527 33.0 25.0 35.0 2.0 35.0 96-97 26.10844472653455 33.0 24.5 35.0 2.0 35.0 98-99 25.400747200152644 33.0 21.0 35.0 2.0 35.0 100-101 22.92131739907034 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2930975014318591 1101 2 0.43128321474867093 1101 3 0.4483965394762208 1101 4 0.5389760035065834 1101 5 0.4393427332999593 1101 6 0.43863008907676004 1101 7 0.564943757750278 1101 8 0.4015182999965816 1101 9 0.7644525930749495 1101 10-11 0.7247999535858085 1101 12-13 0.6586389549299767 1101 14-15 0.9600114405007716 1101 16-17 1.0250535796069684 1101 18-19 0.9557330874472925 1101 20-21 0.7724705445776436 1101 22-23 0.8046468837693084 1101 24-25 0.629222486643279 1101 26-27 0.5168304203938163 1101 28-29 0.6806072493569602 1101 30-31 0.4516512051973862 1101 32-33 0.3937579275470924 1101 34-35 0.5806074710748064 1101 36-37 0.5698692563330425 1101 38-39 0.529428311028056 1101 40-41 0.7090192295045341 1101 42-43 0.8150378804119285 1101 44-45 0.8718145037273786 1101 46-47 0.9390654101015841 1101 48-49 0.9484262346428025 1101 50-51 0.7703833797574617 1101 52-53 0.8355601403712569 1101 54-55 0.8766083304519512 1101 56-57 1.1472565588902555 1101 58-59 0.8179638801464364 1101 60-61 0.7845449433727225 1101 62-63 0.6922496875999897 1101 64-65 0.4821254928836822 1101 66-67 0.6354762832550662 1101 68-69 0.8948296796204112 1101 70-71 0.4686682272678233 1101 72-73 0.6108507257077669 1101 74-75 0.4656200375874455 1101 76-77 0.4159177656641617 1101 78-79 0.4039734689395189 1101 80-81 0.6456652239121148 1101 82-83 0.8569308289566244 1101 84-85 0.7563072217666225 1101 86-87 0.5813832846761251 1101 88-89 0.6650391451755837 1101 90-91 0.8870670949299502 1101 92-93 0.7785474632207929 1101 94-95 0.8442432690804225 1101 96-97 0.5489190265700792 1101 98-99 0.5155832288944246 1101 100-101 0.7299263887855822 1102 1 0.32805887659402444 1102 2 0.5435827194072367 1102 3 0.6039626018488029 1102 4 0.5148618054037115 1102 5 0.3969754561614849 1102 6 0.27205363127344384 1102 7 0.5040043359855844 1102 8 0.354340819498546 1102 9 0.17893774968034393 1102 10-11 0.46088585067609955 1102 12-13 0.44653246915840583 1102 14-15 0.632988691018852 1102 16-17 0.5289039536402953 1102 18-19 0.3893337479025334 1102 20-21 0.5737927186884733 1102 22-23 0.46401593419641785 1102 24-25 0.621858179026141 1102 26-27 0.20317156995660213 1102 28-29 0.17923674154774716 1102 30-31 0.14594424851448906 1102 32-33 0.47056533408088086 1102 34-35 0.20717068186448273 1102 36-37 0.3462353414508996 1102 38-39 0.07188509501538931 1102 40-41 0.44133478221458944 1102 42-43 0.3061377405706338 1102 44-45 0.4260272225453008 1102 46-47 0.7593656687237313 1102 48-49 1.1262489647491165 1102 50-51 0.849837029952063 1102 52-53 1.0282489050503827 1102 54-55 1.0202415503397404 1102 56-57 1.0721924298656091 1102 58-59 0.7978606418939478 1102 60-61 0.8913950604687386 1102 62-63 0.8418624915020629 1102 64-65 0.8328422847897414 1102 66-67 0.6835398815567473 1102 68-69 0.6949213978149409 1102 70-71 0.48540442847079035 1102 72-73 0.7236904305247087 1102 74-75 0.5601703341183715 1102 76-77 0.6986256742787234 1102 78-79 0.5666526932415792 1102 80-81 0.7526016887917351 1102 82-83 0.4510325382215292 1102 84-85 0.35386705446252975 1102 86-87 0.33378891286678325 1102 88-89 0.47461700981595456 1102 90-91 0.5218049135743357 1102 92-93 0.34266279858715976 1102 94-95 0.21177839788604302 1102 96-97 0.4197132576957152 1102 98-99 0.7001163401103732 1102 100-101 1.29960957666151 1103 1 0.31932698513461943 1103 2 0.4395756462240499 1103 3 0.42786183239542197 1103 4 0.35529811270841094 1103 5 0.36405876821568484 1103 6 0.37036640896610606 1103 7 0.27929777520387233 1103 8 0.29817880870577085 1103 9 0.3081095233384801 1103 10-11 0.24376902399036027 1103 12-13 0.1720205060308828 1103 14-15 0.5582260597266213 1103 16-17 0.6510211633503857 1103 18-19 0.7691832618871786 1103 20-21 0.7179864949682013 1103 22-23 0.4014132623887079 1103 24-25 0.49868446045542214 1103 26-27 0.47626634295082937 1103 28-29 0.5119939424057307 1103 30-31 0.316379449304371 1103 32-33 0.4778215177963929 1103 34-35 0.502983055253651 1103 36-37 0.5141984391816905 1103 38-39 0.134351048405577 1103 40-41 0.5652325232220292 1103 42-43 0.600211076968165 1103 44-45 0.3792163259278283 1103 46-47 0.5863030272175038 1103 48-49 0.9714273264262587 1103 50-51 0.9406957280097004 1103 52-53 0.8533371070489153 1103 54-55 0.7501423470304118 1103 56-57 0.90500176459485 1103 58-59 0.7029440135297165 1103 60-61 0.7338848039275376 1103 62-63 0.8299371344382465 1103 64-65 0.7901919902865657 1103 66-67 0.8064348435096704 1103 68-69 0.5994350207146901 1103 70-71 0.8325154672328559 1103 72-73 0.8115030209610161 1103 74-75 0.8225563843711647 1103 76-77 0.5345646372189883 1103 78-79 0.4950952310758723 1103 80-81 0.49640946163275146 1103 82-83 0.6639807831187916 1103 84-85 0.7699160943481331 1103 86-87 0.4917401794006686 1103 88-89 0.261619290938814 1103 90-91 0.4979909059478942 1103 92-93 0.9484105046968239 1103 94-95 0.7780809786190659 1103 96-97 0.7683888445741509 1103 98-99 1.0477593571506993 1103 100-101 1.6670645287409087 1104 1 0.13110480259729584 1104 2 0.06796622502834282 1104 3 0.3277996593824781 1104 4 0.19276198291232305 1104 5 0.29279791274148437 1104 6 0.13853713341399043 1104 7 0.21490240775474945 1104 8 0.0892047295608478 1104 9 0.5816277883145702 1104 10-11 0.4066771997415657 1104 12-13 0.3720125690505114 1104 14-15 0.3260412735951661 1104 16-17 0.17579777042355715 1104 18-19 0.2980821381430374 1104 20-21 0.47777709096928334 1104 22-23 0.3220318838675027 1104 24-25 0.49929130874664907 1104 26-27 0.15574131169897498 1104 28-29 0.0075591546207434135 1104 30-31 0.10804528920225209 1104 32-33 0.10575902730423792 1104 34-35 0.20806801401442243 1104 36-37 0.34508784397430503 1104 38-39 0.0830120136746757 1104 40-41 0.24654953283238967 1104 42-43 0.5293983301776848 1104 44-45 0.6401230597942629 1104 46-47 0.7177261939403934 1104 48-49 0.6795994398457026 1104 50-51 0.5863955381835879 1104 52-53 0.48595247436575306 1104 54-55 0.47630164222655225 1104 56-57 0.6250132070748862 1104 58-59 0.5606190735531342 1104 60-61 0.7094008730057269 1104 62-63 0.5451566250163822 1104 64-65 0.4376147866170328 1104 66-67 0.5455214954929204 1104 68-69 0.5952631454046475 1104 70-71 0.6252957914585018 1104 72-73 0.7836822512062049 1104 74-75 0.7355373157758116 1104 76-77 0.5659140561658873 1104 78-79 0.6421112571620782 1104 80-81 0.6569993776991865 1104 82-83 0.7064915807191774 1104 84-85 0.7054330894073608 1104 86-87 0.3162436220659046 1104 88-89 0.5296577323804321 1104 90-91 0.8613673635412695 1104 92-93 0.6522652314993458 1104 94-95 0.7468820315917952 1104 96-97 0.734251561270252 1104 98-99 0.6395273856709167 1104 100-101 1.3938207510028136 1105 1 0.24804928468610754 1105 2 0.11144546792485244 1105 3 0.33652712922934214 1105 4 0.3580079512007117 1105 5 0.31152899644749255 1105 6 0.29859269649841735 1105 7 0.5630774088840838 1105 8 0.2563028006635264 1105 9 0.1017795703988682 1105 10-11 0.40658911192478087 1105 12-13 0.39978281795506376 1105 14-15 0.6982202047963284 1105 16-17 0.7135242012859138 1105 18-19 0.5561195867584772 1105 20-21 0.5804547347331166 1105 22-23 0.4504063248486645 1105 24-25 0.2957790893854053 1105 26-27 0.35825746113204104 1105 28-29 0.46487721939916327 1105 30-31 0.10172555301397068 1105 32-33 0.35109376100798784 1105 34-35 0.24094509763008887 1105 36-37 0.12192639481678924 1105 38-39 -0.07835470268255307 1105 40-41 0.005679044509669495 1105 42-43 0.18317820098221915 1105 44-45 0.14572115348356363 1105 46-47 0.6068913924767827 1105 48-49 0.8062934363221856 1105 50-51 0.7443042430668143 1105 52-53 1.0193829301637365 1105 54-55 0.9047506190526846 1105 56-57 0.7599038878356055 1105 58-59 0.7539515034216748 1105 60-61 0.6200312655611739 1105 62-63 0.6786932379269004 1105 64-65 1.075428175965186 1105 66-67 0.990252037120058 1105 68-69 0.8195268216028744 1105 70-71 0.8291151888474921 1105 72-73 0.7470772460112833 1105 74-75 0.8181817572087091 1105 76-77 0.5349048859982943 1105 78-79 0.6507562858470877 1105 80-81 0.8567610888614894 1105 82-83 0.7337649239906234 1105 84-85 0.876091941103617 1105 86-87 0.8884824078092315 1105 88-89 0.8149030230702259 1105 90-91 0.7891466470936912 1105 92-93 0.8255238031215164 1105 94-95 0.7975867341260141 1105 96-97 0.9347202336287488 1105 98-99 1.0361710139855056 1105 100-101 1.4696937238536947 1106 1 0.13372541750904077 1106 2 0.08042080843940624 1106 3 0.2719139359595104 1106 4 0.22590165724540157 1106 5 0.24651074664780026 1106 6 0.16869863436007648 1106 7 0.2724426719482693 1106 8 0.3052033615110332 1106 9 0.4167755643452722 1106 10-11 0.3225554610746073 1106 12-13 0.3581755002304945 1106 14-15 0.3768390065411822 1106 16-17 0.11749241992741588 1106 18-19 0.44464446880245845 1106 20-21 0.319702932009001 1106 22-23 0.012195250336070274 1106 24-25 -0.004254294394534952 1106 26-27 -0.1570790473696917 1106 28-29 0.09317331127132888 1106 30-31 -0.16644857678216596 1106 32-33 0.05314129951061375 1106 34-35 0.2051064970781411 1106 36-37 -0.0268762402762448 1106 38-39 0.05402978943887149 1106 40-41 0.004319211873685447 1106 42-43 0.16342390560253506 1106 44-45 0.23129936055202194 1106 46-47 0.47483183581337585 1106 48-49 0.39492737204728456 1106 50-51 0.3640741175438009 1106 52-53 0.3039729773461488 1106 54-55 0.749110985829148 1106 56-57 0.7588200277804162 1106 58-59 0.41404230730309877 1106 60-61 0.5373841543709261 1106 62-63 0.4199778457787602 1106 64-65 0.21553099800738096 1106 66-67 0.19423806044419223 1106 68-69 0.3519001857976569 1106 70-71 -0.0050720273903799296 1106 72-73 0.3034112618574518 1106 74-75 0.5396163695117444 1106 76-77 0.17997298890935554 1106 78-79 0.31587246140125913 1106 80-81 0.36522944103811383 1106 82-83 0.31636752347428754 1106 84-85 0.6484924574394135 1106 86-87 0.6903741553388301 1106 88-89 0.7703334512728439 1106 90-91 0.8009199934837916 1106 92-93 0.6736654393718489 1106 94-95 0.8110693300487384 1106 96-97 0.9135571427358435 1106 98-99 0.8812480192378835 1106 100-101 1.3449251847279058 1107 1 0.13959085889312917 1107 2 0.18486930293333614 1107 3 0.2770135757332781 1107 4 0.13247386078605672 1107 5 0.25196975700964686 1107 6 0.28358812790424537 1107 7 0.3681528994640786 1107 8 0.5050540515881252 1107 9 0.4967932761813998 1107 10-11 0.4179148744449108 1107 12-13 0.37930028738899324 1107 14-15 0.20511334320296726 1107 16-17 0.23783129783553392 1107 18-19 0.471912781728669 1107 20-21 0.35323135223300994 1107 22-23 0.32142294582939 1107 24-25 0.2256990302112314 1107 26-27 0.22519606792837266 1107 28-29 0.2526290359592309 1107 30-31 0.2879327496375623 1107 32-33 0.17368981026178432 1107 34-35 0.3115646860215122 1107 36-37 0.3127685390103139 1107 38-39 0.4125875154786911 1107 40-41 0.5635522551436196 1107 42-43 0.37760342832416427 1107 44-45 0.34644233870741914 1107 46-47 0.1531299166033051 1107 48-49 0.31235770121363515 1107 50-51 0.36419176862919045 1107 52-53 0.16269755401615527 1107 54-55 0.07051770777042776 1107 56-57 0.19571840291197162 1107 58-59 0.3860948267476161 1107 60-61 0.25503866233270145 1107 62-63 0.3279383321269549 1107 64-65 0.15714183388625713 1107 66-67 0.16633870987818256 1107 68-69 0.2547300152677572 1107 70-71 0.2887679756899395 1107 72-73 0.27842324087704995 1107 74-75 0.21780321654821932 1107 76-77 0.3876677977156504 1107 78-79 0.5538966504644094 1107 80-81 0.45202171628175947 1107 82-83 0.5015171756549002 1107 84-85 0.7421940600611059 1107 86-87 0.7459161631936446 1107 88-89 0.7874794926346915 1107 90-91 0.7493781225320575 1107 92-93 0.8209410680564666 1107 94-95 0.594097198382201 1107 96-97 0.8703150210803408 1107 98-99 0.9491284172835925 1107 100-101 1.2258547994312963 1108 1 0.06450461816271869 1108 2 0.1332832308973657 1108 3 0.197269302737471 1108 4 0.2561131163271497 1108 5 0.2877295361232086 1108 6 0.25810149607814026 1108 7 0.29375176473574527 1108 8 0.44480603632988647 1108 9 0.16247876088529267 1108 10-11 0.11221988776832603 1108 12-13 -0.0054814082311551715 1108 14-15 0.3491938896352593 1108 16-17 0.6037467578205238 1108 18-19 0.38187298361116717 1108 20-21 0.40138328739926266 1108 22-23 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86-87 -4.8396088657029495 2316 88-89 -6.327657877330637 2316 90-91 -5.776055326387656 2316 92-93 -4.933611257007993 2316 94-95 -4.1727339565924595 2316 96-97 -5.908488215338259 2316 98-99 -7.447470675807896 2316 100-101 -7.444012720796319 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1880.0 3 703.0 4 731.0 5 743.0 6 800.0 7 1000.0 8 1306.0 9 1643.0 10 2106.0 11 2790.0 12 3691.0 13 4541.0 14 5062.0 15 6182.0 16 8029.0 17 10498.0 18 8207.0 19 5608.0 20 5523.0 21 5607.0 22 6011.0 23 6885.0 24 7954.0 25 9065.0 26 10323.0 27 12031.0 28 14427.0 29 17094.0 30 20728.0 31 25112.0 32 31828.0 33 39780.0 34 52292.0 35 71980.0 36 98964.0 37 130948.0 38 106011.0 39 16592.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.07903041875514 18.53276579945368 14.86845943723977 21.51974434455141 2 15.385247531314203 20.21817217840811 41.03823977732123 23.358340512956456 3 19.24340514071021 25.953231331959476 30.24298768164439 24.560375845685925 4 12.530780914110315 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26.821497215024493 29.40327253670291 21.88758199727167 50-51 21.762523793402046 27.105032838704417 29.296245329934074 21.836198037959463 52-53 22.063568412167758 27.188965433168626 28.96534041445216 21.782125740211452 54-55 21.663840971323665 27.037498600402955 29.116190074885896 22.182470353387487 56-57 21.817862585304447 27.399059166501026 28.788577486251903 21.99450076194262 58-59 22.11156790708306 26.980365134946215 28.856592935158897 22.051474022811824 60-61 21.8078674547682 26.929511469368528 29.458265226952435 21.804355848910838 62-63 21.70660765515368 27.076081123162236 29.353545659937325 21.86376556174676 64-65 21.580707610235315 27.18910374052083 29.162056551046074 22.068132098197783 66-67 21.472019505020356 27.354002206240413 28.904797082197366 22.269181206541866 68-69 21.525247198068527 27.201725814872436 29.214624372894743 22.058402614164297 70-71 21.6754773784781 27.21698335096801 28.861546493592883 22.24599277696101 72-73 21.650343959714867 27.235738146626705 28.859416199538384 22.254501694120048 74-75 21.560452492525602 27.65049511248701 28.656755581942704 22.13229681304468 76-77 21.721802782330023 27.337555491201925 28.701881946133362 22.238759780334693 78-79 21.718383572518636 27.44319224746717 28.531536743223196 22.306887436791 80-81 21.61652491273453 27.728487106974082 28.45795222331479 22.1970357569766 82-83 21.673885287860326 27.633968532244612 28.59004658478716 22.1020995951079 84-85 21.595150149901446 27.596442118165402 28.22771768837063 22.58069004356252 86-87 21.68695932171273 27.421022956528425 28.362963458644924 22.529054263113924 88-89 21.71262595712174 27.322881545149112 28.29303875924823 22.671453738480913 90-91 21.516385703580465 27.41068050644251 28.6043015412056 22.468632248771428 92-93 21.699519025302358 27.285027368315042 28.610209067290736 22.405244539091868 94-95 21.351215963169256 27.219922951230274 28.63897734787374 22.78988373772673 96-97 21.37482523969494 27.021355543628783 28.76253139059508 22.841287826081196 98-99 21.29080099030372 27.29425220936335 28.393234868445465 23.021711931887463 100-101 21.215237650950495 27.28201634877384 28.513409281268885 22.989336719006776 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 205.0 1 164.5 2 286.0 3 382.5 4 280.5 5 261.0 6 406.5 7 822.5 8 1343.5 9 1592.5 10 1465.5 11 1270.0 12 1210.0 13 1284.5 14 1405.0 15 1481.5 16 1544.0 17 1652.5 18 1773.5 19 1891.5 20 2063.0 21 2283.5 22 2628.0 23 3036.0 24 3451.5 25 4061.5 26 5089.0 27 6155.5 28 7112.0 29 8341.5 30 9817.5 31 11738.0 32 13777.0 33 15250.0 34 16588.0 35 17724.5 36 19215.5 37 20905.0 38 21377.0 39 21303.5 40 21611.0 41 22498.0 42 23466.5 43 24498.0 44 25497.0 45 26466.0 46 26725.0 47 27179.5 48 29859.5 49 29612.5 50 26899.5 51 25302.0 52 23791.5 53 23216.5 54 22442.0 55 20855.0 56 19062.0 57 17149.5 58 15748.0 59 14063.0 60 11872.0 61 9939.0 62 8254.0 63 6514.0 64 4822.5 65 3682.0 66 2732.5 67 2090.5 68 1595.5 69 1108.5 70 828.5 71 645.5 72 521.0 73 453.5 74 347.0 75 260.0 76 194.0 77 112.0 78 76.5 79 53.5 80 31.5 81 19.5 82 10.0 83 9.0 84 12.0 85 6.5 86 2.5 87 2.5 88 1.5 89 1.5 90 1.5 91 0.5 92 0.5 93 1.5 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07208315003365648 2 0.031006354977712525 3 0.015768189069862354 4 0.020935914899481108 5 0.026501158100608997 6 0.0276937102151364 7 0.07698586428226914 8 0.05525491463976976 9 0.06413280260347377 10-11 0.05777252465932761 12-13 0.03730038002660716 14-15 0.0539298567347393 16-17 0.06115142231715526 18-19 0.057971283345082184 20-21 0.01669572960338367 22-23 0.013979360898071246 24-25 0.021532190956744808 26-27 0.024381065452560274 28-29 0.006824048210906817 30-31 0.0013913108002819722 32-33 0.009076646649458581 34-35 0.010732969030746643 36-37 0.004107679505594394 38-39 0.009209152439961628 40-41 0.004637702667606574 42-43 0.0026501158100608996 44-45 6.625289525152249E-4 46-47 9.275405335213149E-4 48-49 0.0012588050097789272 50-51 0.0013913108002819722 52-53 1.32505790503045E-4 54-55 3.3126447625761245E-4 56-57 0.00450519687710353 58-59 0.004173932400845917 60-61 0.005764001886882457 62-63 0.0038426679245883045 64-65 3.9751737150913493E-4 66-67 8.612876382697923E-4 68-69 0.0034451505530791694 70-71 0.002053839752797197 72-73 0.021532190956744808 74-75 0.014310625374328858 76-77 0.02285724886177526 78-79 0.019014580937186956 80-81 0.011925521145274048 82-83 0.005101472934367232 84-85 0.002848874495815467 86-87 0.0024513571243063325 88-89 0.0016563223812880623 90-91 0.006294025048894637 92-93 0.00934165823046467 94-95 0.012853061678795363 96-97 0.010467957449740554 98-99 0.022260972804511555 100-101 0.017490764346401937 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 754684.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.44716182751313 #Duplication Level Percentage of deduplicated Percentage of total 1 85.06959928402694 56.526334302274336 2 9.040150997580419 12.01384752562761 3 2.450944287371379 4.885748750795547 4 1.0426021363604026 2.7711181150580235 5 0.5735193367571617 1.905436609035556 6 0.3619888316439488 1.4431878285598725 7 0.24436540686688016 1.1366171414590773 8 0.18060863786368886 0.9600745110060201 9 0.1329520208851867 0.7950856002347616 >10 0.8233708801301788 10.157820062089156 >50 0.05166393675188788 2.349943529351406 >100 0.026632300855154417 3.066002484709983 >500 8.00971453398643E-4 0.3524645478642289 >1k 8.00971453398643E-4 1.6363189919344232 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4868 0.645038188168823 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3957 0.524325413020549 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2300 0.30476331815700347 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1173 0.15542929226007177 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 823 0.10905226558400602 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.62528952515225E-5 1.32505790503045E-4 20-21 0.0 0.0 0.0 1.32505790503045E-4 1.32505790503045E-4 22-23 0.0 0.0 0.0 1.987586857545675E-4 1.32505790503045E-4 24-25 0.0 0.0 0.0 5.3002316201218E-4 1.32505790503045E-4 26-27 0.0 0.0 0.0 8.612876382697923E-4 1.32505790503045E-4 28-29 0.0 0.0 0.0 0.0019875868575456746 1.32505790503045E-4 30-31 0.0 0.0 0.0 0.004438943981852007 1.32505790503045E-4 32-33 0.0 0.0 0.0 0.006691542420403772 1.32505790503045E-4 34-35 0.0 0.0 0.0 0.009937934287728375 1.32505790503045E-4 36-37 1.32505790503045E-4 0.0 0.0 0.013184326155052977 1.32505790503045E-4 38-39 1.32505790503045E-4 0.0 0.0 0.018285799089420206 1.32505790503045E-4 40-41 1.32505790503045E-4 0.0 0.0 0.02285724886177526 1.32505790503045E-4 42-43 2.6501158100609E-4 0.0 0.0 0.031470125244473186 1.32505790503045E-4 44-45 2.6501158100609E-4 0.0 0.0 0.04544948614254443 1.32505790503045E-4 46-47 3.9751737150913493E-4 0.0 0.0 0.056778731230554776 2.6501158100609E-4 48-49 3.9751737150913493E-4 0.0 0.0 0.07102310370963211 2.6501158100609E-4 50-51 3.9751737150913493E-4 0.0 0.0 0.08911014411329775 2.6501158100609E-4 52-53 3.9751737150913493E-4 0.0 0.0 0.11965272882424963 2.6501158100609E-4 54-55 3.9751737150913493E-4 2.6501158100609E-4 0.0 0.14158243715250357 2.6501158100609E-4 56-57 3.9751737150913493E-4 2.6501158100609E-4 0.0 0.1918021317531576 2.6501158100609E-4 58-59 3.9751737150913493E-4 2.6501158100609E-4 0.0 0.25765750963317097 2.6501158100609E-4 60-61 3.9751737150913493E-4 2.6501158100609E-4 0.0 0.31649008061652295 2.6501158100609E-4 62-63 3.9751737150913493E-4 2.6501158100609E-4 0.0 0.3965235780803621 2.6501158100609E-4 64-65 3.9751737150913493E-4 2.6501158100609E-4 0.0 0.4555549077494686 2.6501158100609E-4 66-67 3.9751737150913493E-4 2.6501158100609E-4 0.0 0.5595057003991075 2.6501158100609E-4 68-69 3.9751737150913493E-4 2.6501158100609E-4 0.0 0.6808147516046452 2.6501158100609E-4 70-71 3.9751737150913493E-4 2.6501158100609E-4 0.0 0.7656184575265939 2.6501158100609E-4 72-73 3.9751737150913493E-4 2.6501158100609E-4 0.0 0.916145035538053 3.9751737150913493E-4 74-75 3.9751737150913493E-4 2.6501158100609E-4 0.0 1.0566011734712806 3.9751737150913493E-4 76-77 3.9751737150913493E-4 2.6501158100609E-4 0.0 1.1444525125747995 3.9751737150913493E-4 78-79 3.9751737150913493E-4 2.6501158100609E-4 0.0 1.2437656025568318 3.9751737150913493E-4 80-81 6.625289525152249E-4 2.6501158100609E-4 0.0 1.4338451590334498 3.9751737150913493E-4 82-83 6.625289525152249E-4 2.6501158100609E-4 0.0 1.59225583157984 3.9751737150913493E-4 84-85 6.625289525152249E-4 2.6501158100609E-4 0.0 1.8354702100481792 3.9751737150913493E-4 86-87 6.625289525152249E-4 2.6501158100609E-4 0.0 2.1952234312639463 3.9751737150913493E-4 88-89 6.625289525152249E-4 2.6501158100609E-4 0.0 2.6621738369966765 3.9751737150913493E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3660 0.0 33.76699 1 GTATCAA 5810 0.0 33.707085 1 TAACGCA 70 0.00836094 27.16202 4 TATCAAC 7160 0.0 27.086147 2 ATCAACG 7115 0.0 27.057035 3 TACCTGG 795 0.0 26.905773 2 TCAACGC 7175 0.0 26.764526 4 CAACGCA 7240 0.0 26.591656 5 AACGCAG 7400 0.0 26.08094 6 GTACATG 4595 0.0 25.964998 1 GTACCTG 905 0.0 25.736387 1 TACATGG 4625 0.0 24.974377 2 ACATGGG 4620 0.0 24.384087 3 ACGCAGA 8160 0.0 23.53532 7 GAGTACT 4270 0.0 23.494701 12-13 CGCAGAG 8210 0.0 23.456112 8 GTATAGG 225 2.7866918E-9 23.238617 1 CATGGGA 2775 0.0 22.439255 4 GCAGAGT 8520 0.0 22.270763 9 CATTCCG 150 3.0041578E-5 22.192621 9 >>END_MODULE