FastQCFastQC Report
Thu 26 May 2016
SRR936636_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936636_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754684
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52420.6945953538169618No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47490.6292699990989606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27580.3654509702073981No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14360.1902783151623726No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12470.1652347207572971No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7610.10083690657281723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG8250.032.2442672
GGTATCA48850.031.5147631
GTACCTG9050.030.4518031
TCGTTAG804.4976667E-429.681339
GTATCAA69100.028.1928791
ACGTCTA1701.1232987E-725.1434975
GTACCCG1151.2783812E-424.79061
GTACATG47350.023.7827451
GTAAGGT3200.023.7466374
GAGTACT48200.023.74506412-13
CGTCTAT1801.9543586E-723.743496
ATCAACG82950.023.418973
TATCAAC83000.023.4079652
TCAACGC83650.023.2214584
CAACGCA84950.022.8660965
CTACGTC2103.444984E-822.6173443
AACGCAG86300.022.5604446
TACATGG48900.022.342792
GTACTTT52050.021.89746314-15
GTCTATC1754.0558243E-621.7083347
Produced by FastQC (version 0.11.5)