##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936636_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 754684 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.886286180706097 31.0 31.0 34.0 30.0 34.0 2 32.035666583629705 31.0 31.0 34.0 30.0 34.0 3 32.09739043096183 33.0 31.0 34.0 30.0 34.0 4 35.816876997524794 37.0 35.0 37.0 35.0 37.0 5 35.64968914141548 37.0 35.0 37.0 33.0 37.0 6 35.75477815880554 37.0 35.0 37.0 33.0 37.0 7 35.69574947925224 37.0 35.0 37.0 33.0 37.0 8 35.67279152598969 37.0 35.0 37.0 33.0 37.0 9 37.29149551335394 39.0 37.0 39.0 34.0 39.0 10-11 37.19159807283578 39.0 37.0 39.0 33.0 39.0 12-13 37.08204029766101 39.0 37.0 39.0 33.0 39.0 14-15 38.183448966719844 40.0 37.5 41.0 33.0 41.0 16-17 38.09750637352852 40.0 37.0 41.0 33.0 41.0 18-19 38.26630748763721 40.0 38.0 41.0 33.5 41.0 20-21 38.30307983208866 40.0 38.0 41.0 34.0 41.0 22-23 38.21186549602217 40.0 37.5 41.0 33.0 41.0 24-25 38.0760589862777 40.0 37.0 41.0 33.0 41.0 26-27 37.86719607146832 40.0 37.0 41.0 33.0 41.0 28-29 37.761132473989115 40.0 37.0 41.0 33.0 41.0 30-31 37.670527664558946 39.5 37.0 41.0 33.0 41.0 32-33 37.453106200740976 39.0 36.0 41.0 32.5 41.0 34-35 37.253812854121726 39.0 36.0 41.0 32.0 41.0 36-37 37.01302399414855 39.0 35.0 41.0 31.0 41.0 38-39 36.8314725103487 39.0 35.0 41.0 31.0 41.0 40-41 36.617678392545756 39.0 35.0 41.0 30.5 41.0 42-43 36.30358335409258 39.0 35.0 40.5 30.0 41.0 44-45 35.946494161794874 39.0 35.0 40.0 29.5 41.0 46-47 35.432908343094596 38.5 35.0 40.0 27.5 41.0 48-49 34.93569295228201 38.0 34.5 40.0 25.5 41.0 50-51 34.61050254146106 38.0 34.0 40.0 24.0 41.0 52-53 34.23123718006477 38.0 34.0 40.0 23.0 41.0 54-55 34.04182412771438 38.0 33.0 40.0 23.0 41.0 56-57 33.743898770876285 38.0 33.0 40.0 22.0 41.0 58-59 33.37748713368774 37.0 33.0 40.0 20.5 41.0 60-61 32.968589502361255 36.5 32.0 39.5 19.0 41.0 62-63 32.65021121423007 36.0 32.0 39.0 18.0 40.5 64-65 32.21943687689152 35.5 31.0 39.0 17.0 40.0 66-67 31.817974145470156 35.0 31.0 38.5 13.5 40.0 68-69 31.472848900996972 35.0 31.0 38.0 12.5 40.0 70-71 31.11718812112089 35.0 30.5 37.5 8.0 39.5 72-73 30.671842784529684 34.5 30.0 37.0 7.0 39.0 74-75 30.168771300305824 34.0 29.0 36.0 6.0 39.0 76-77 28.685468354967114 32.5 27.0 35.0 2.0 37.0 78-79 29.446532588474113 34.0 29.0 35.0 2.0 37.0 80-81 29.215279507714488 34.0 29.0 35.0 2.0 37.0 82-83 28.933925325036704 34.0 29.0 35.0 2.0 36.5 84-85 28.574609372929597 34.0 29.0 35.0 2.0 36.0 86-87 28.187205505880605 33.5 28.0 35.0 2.0 36.0 88-89 27.731160326706277 33.0 27.0 35.0 2.0 35.0 90-91 27.273900731961987 33.0 26.0 35.0 2.0 35.0 92-93 26.656091158683637 32.5 25.0 34.5 2.0 35.0 94-95 26.14012222334116 32.0 24.5 34.0 2.0 35.0 96-97 25.46120164201176 32.0 21.5 34.0 2.0 35.0 98-99 24.6295442860853 32.0 10.5 34.0 2.0 35.0 100-101 21.527603606277594 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3320310360992842 1101 2 0.32178373477398736 1101 3 0.3063195102243341 1101 4 0.24418451192344293 1101 5 0.2816366678984963 1101 6 0.20860517355334451 1101 7 0.18212322039970275 1101 8 0.18914029713255331 1101 9 0.2889382489513963 1101 10-11 0.3779719443803202 1101 12-13 0.3327558696115389 1101 14-15 0.5168476132390154 1101 16-17 0.45415060212633307 1101 18-19 0.3591045072901551 1101 20-21 0.3745553160597126 1101 22-23 0.3610282943160996 1101 24-25 0.3535860390199659 1101 26-27 0.47687503452369384 1101 28-29 0.3701638441643169 1101 30-31 0.5144332433715348 1101 32-33 0.38178782987031923 1101 34-35 0.43156735174207483 1101 36-37 0.36870931877393076 1101 38-39 0.5052873568995082 1101 40-41 0.4931013520287806 1101 42-43 0.3855718043609144 1101 44-45 0.6204544879357528 1101 46-47 0.6839909803809192 1101 48-49 0.7697679389418965 1101 50-51 0.6426973005265779 1101 52-53 0.734326193751329 1101 54-55 0.5175594652960953 1101 56-57 0.5229236600352323 1101 58-59 0.5068076070843475 1101 60-61 0.8092536558139543 1101 62-63 0.8154599447027095 1101 64-65 0.8211721380597723 1101 66-67 0.4997244322941583 1101 68-69 0.519122928577211 1101 70-71 0.4276730103743027 1101 72-73 0.7257811612032157 1101 74-75 0.8589127239499916 1101 76-77 0.6792638978796681 1101 78-79 0.6287648993679049 1101 80-81 0.6125126277359492 1101 82-83 0.6839276513846961 1101 84-85 0.48945159505311153 1101 86-87 0.5032535621613405 1101 88-89 0.5923539279623071 1101 90-91 0.8745230654106706 1101 92-93 0.6520615096146329 1101 94-95 0.6565637759480722 1101 96-97 0.900010927607557 1101 98-99 0.72082296087952 1101 100-101 0.9684588088445274 1102 1 0.17564256373900022 1102 2 0.21213152679798242 1102 3 0.2948858000496202 1102 4 0.28399505224996346 1102 5 0.27574501120043493 1102 6 0.1811244649118251 1102 7 0.24298787762005958 1102 8 0.2777004794316795 1102 9 0.35791643876488166 1102 10-11 0.38498612226517537 1102 12-13 0.35099736540787063 1102 14-15 0.5061278518116197 1102 16-17 0.42700686044520353 1102 18-19 0.3106376016577528 1102 20-21 0.31153564982548687 1102 22-23 0.34853419123790985 1102 24-25 0.3015799272817219 1102 26-27 0.40668692271805895 1102 28-29 0.38723437977329667 1102 30-31 0.4811203737609091 1102 32-33 0.6057234354841938 1102 34-35 0.35531962626905766 1102 36-37 0.33279127335078584 1102 38-39 0.4100678650456828 1102 40-41 0.43446436232311925 1102 42-43 0.5151782480116154 1102 44-45 0.5793697912902545 1102 46-47 0.3881850607138446 1102 48-49 0.5849799964621525 1102 50-51 0.7164683188407395 1102 52-53 0.6962168100638948 1102 54-55 0.5652660892241173 1102 56-57 0.3241205245576637 1102 58-59 0.5581487355866983 1102 60-61 0.599143699282692 1102 62-63 0.45614786275234565 1102 64-65 0.2720692072896327 1102 66-67 0.015403926169785365 1102 68-69 0.49481550098298754 1102 70-71 0.49897206012747475 1102 72-73 0.6577022519652687 1102 74-75 0.24431026072150885 1102 76-77 0.27419117619752953 1102 78-79 0.5765881981059628 1102 80-81 0.7542009164492391 1102 82-83 0.5747392994901013 1102 84-85 0.5121442137918457 1102 86-87 0.7335952255987408 1102 88-89 0.7094741661979498 1102 90-91 0.6418684396967222 1102 92-93 0.6504303789219996 1102 94-95 0.5125351463927039 1102 96-97 0.5498074838052212 1102 98-99 0.25650723547389376 1102 100-101 0.7092622433802696 1103 1 0.1296604892790647 1103 2 0.14463093098230928 1103 3 0.08499483431753418 1103 4 0.13253658060227735 1103 5 0.20289058599232135 1103 6 0.06572241700824577 1103 7 0.05196086163518032 1103 8 0.016295929102277285 1103 9 0.11094339516819929 1103 10-11 0.18483993463431148 1103 12-13 0.23824669724314873 1103 14-15 0.11034878026267592 1103 16-17 0.265131190152573 1103 18-19 0.16337484269072178 1103 20-21 0.05543864677832033 1103 22-23 0.04134759028120527 1103 24-25 0.15648657573345304 1103 26-27 -0.10532089097889497 1103 28-29 -0.05181899679922708 1103 30-31 0.09149575891317596 1103 32-33 0.00964815842989708 1103 34-35 -0.2341724195917294 1103 36-37 -0.09855958973077605 1103 38-39 -0.1685297976545499 1103 40-41 0.14031388907523734 1103 42-43 0.10434685107920671 1103 44-45 -0.04119776569525868 1103 46-47 0.08190229085190737 1103 48-49 -0.16358951394759913 1103 50-51 -0.13490918571299915 1103 52-53 -0.20182902092872723 1103 54-55 -0.19085751273696872 1103 56-57 -0.29454999788756453 1103 58-59 -0.3815930208779008 1103 60-61 -0.0906517869880119 1103 62-63 0.06308400833565031 1103 64-65 -0.16766669741390317 1103 66-67 -0.46048043452000087 1103 68-69 -0.4164116877884396 1103 70-71 -0.4957879538316341 1103 72-73 -0.5341876327869599 1103 74-75 -0.3108622704736952 1103 76-77 -0.5322245882339764 1103 78-79 -0.5376440295637153 1103 80-81 -0.3440044016312136 1103 82-83 -0.3106454484798711 1103 84-85 -0.044809380678987765 1103 86-87 -0.11396161096051216 1103 88-89 -0.3943520343706268 1103 90-91 -0.48712139739024707 1103 92-93 -0.44194339327213683 1103 94-95 -0.6524637683716108 1103 96-97 -0.21975867726928655 1103 98-99 -0.2576396628799493 1103 100-101 0.3129581092706708 1104 1 0.21691766728569561 1104 2 0.25654566135646917 1104 3 0.1268921694763705 1104 4 0.17464887564629095 1104 5 0.2725276675646384 1104 6 0.14082609381440392 1104 7 0.0991362887439351 1104 8 0.18118669640985985 1104 9 0.10882355999282112 1104 10-11 0.2124655869548846 1104 12-13 0.19566645106447567 1104 14-15 0.3679930844871322 1104 16-17 0.22317432764091905 1104 18-19 0.2005463674507979 1104 20-21 0.19009778654192644 1104 22-23 0.2315242542360636 1104 24-25 0.20337263376600845 1104 26-27 0.21346792581575613 1104 28-29 0.2199564395693372 1104 30-31 0.24564845318730733 1104 32-33 -0.0176798571265806 1104 34-35 0.02479140762021359 1104 36-37 0.10110011223561344 1104 38-39 0.22731883090137472 1104 40-41 0.12141726163049071 1104 42-43 0.12517811749057017 1104 44-45 0.107736363981779 1104 46-47 0.11407021090005998 1104 48-49 0.05124139529964822 1104 50-51 0.11049067274419144 1104 52-53 0.07545765618829137 1104 54-55 -0.04787281126664311 1104 56-57 -0.20372613932455153 1104 58-59 -0.1484921749280801 1104 60-61 -0.0024636673248039642 1104 62-63 -0.2065335614932451 1104 64-65 -0.20088792089942942 1104 66-67 -0.5159863839482028 1104 68-69 -0.42233300585631284 1104 70-71 -0.3912314656808853 1104 72-73 -0.25482907308433056 1104 74-75 -0.11776380561798305 1104 76-77 -0.2609766303528893 1104 78-79 -0.21070492604564706 1104 80-81 -0.07606517244429156 1104 82-83 -0.018619137389922713 1104 84-85 0.31638440731103756 1104 86-87 -0.011685351130232391 1104 88-89 -0.193594383452254 1104 90-91 -0.11514489085216084 1104 92-93 0.10919225633663743 1104 94-95 -0.025591137064193248 1104 96-97 0.03669011928606025 1104 98-99 -0.05895885515991495 1104 100-101 0.9335158805665849 1105 1 0.03308065233335 1105 2 0.06776895267869065 1105 3 0.09329005536876878 1105 4 0.1692845534707459 1105 5 0.17552265333506512 1105 6 0.18629973522923393 1105 7 0.2252690077212094 1105 8 0.17341464057573575 1105 9 0.2128213916773376 1105 10-11 0.13988235526134218 1105 12-13 0.20047853038868624 1105 14-15 0.26800437779697717 1105 16-17 0.10086891136951692 1105 18-19 0.1206371656619396 1105 20-21 0.040065035943385396 1105 22-23 0.24415243330278713 1105 24-25 0.21990265138357046 1105 26-27 0.15085329872084685 1105 28-29 0.17732419491107265 1105 30-31 0.2018236350095961 1105 32-33 0.039050955539991605 1105 34-35 0.09390307586794222 1105 36-37 -0.02106074966979321 1105 38-39 -0.11888661699128988 1105 40-41 0.023980842964903104 1105 42-43 0.02870501815461779 1105 44-45 0.11416225728257956 1105 46-47 0.09917433521680863 1105 48-49 0.32017575858283465 1105 50-51 0.3756700105044999 1105 52-53 0.4366052823345683 1105 54-55 0.13173147115645634 1105 56-57 0.3435092584258186 1105 58-59 0.20021361176388552 1105 60-61 0.2499350316859008 1105 62-63 0.04107112748899766 1105 64-65 0.12135199417487996 1105 66-67 0.060006297987015955 1105 68-69 0.2717447824618837 1105 70-71 0.14472982085296948 1105 72-73 0.3469462352230295 1105 74-75 0.2609042614191033 1105 76-77 0.07645617445354347 1105 78-79 0.09490220229152513 1105 80-81 0.37191106643179594 1105 82-83 0.48578525344894175 1105 84-85 0.5382516431605211 1105 86-87 0.5187343082635465 1105 88-89 0.6642657601986457 1105 90-91 0.8381188756925368 1105 92-93 0.6701591895254175 1105 94-95 0.8488950173379415 1105 96-97 0.7391430261840135 1105 98-99 0.14198639487396392 1105 100-101 0.6926442629128822 1106 1 0.1067163685267083 1106 2 0.1887117106240339 1106 3 0.03599458666050026 1106 4 0.03897653246122701 1106 5 0.13580485755478122 1106 6 -0.12195273517048122 1106 7 -0.1152499987775002 1106 8 0.019199381962721418 1106 9 0.10641170592998606 1106 10-11 0.08599870886234129 1106 12-13 0.079909670778612 1106 14-15 0.22513292760557846 1106 16-17 0.20881202540851262 1106 18-19 0.15013062244107545 1106 20-21 -0.012605666451349862 1106 22-23 -0.12265296151605298 1106 24-25 -0.025463679961568175 1106 26-27 -0.15002135631342384 1106 28-29 -0.2936207886195916 1106 30-31 -0.1253062501502029 1106 32-33 8.465577833902671E-4 1106 34-35 -0.17892697843633698 1106 36-37 -0.08109813950331812 1106 38-39 0.08666176723375685 1106 40-41 0.007538218554678622 1106 42-43 -0.029850131849833872 1106 44-45 -0.27540419869533395 1106 46-47 -0.11917784140563725 1106 48-49 -0.32228173273738037 1106 50-51 -0.24712443443452514 1106 52-53 -0.4442613605925274 1106 54-55 -0.29138345806993016 1106 56-57 -0.41011622007503234 1106 58-59 -0.3289148490213023 1106 60-61 -0.20935011030643835 1106 62-63 -0.1994612325216849 1106 64-65 -0.32833419829373867 1106 66-67 -0.08714940396259863 1106 68-69 -0.40144581666568513 1106 70-71 -0.3625968060499041 1106 72-73 -0.21997711561824218 1106 74-75 -0.1529477816682281 1106 76-77 -0.13786769272352117 1106 78-79 -0.03538578845003926 1106 80-81 -0.02029493374624991 1106 82-83 -0.11117237140639347 1106 84-85 0.1418170308574851 1106 86-87 0.025802767268263693 1106 88-89 -0.0024016483297941704 1106 90-91 0.09356265740712644 1106 92-93 0.1451102266481783 1106 94-95 0.21382424372447772 1106 96-97 0.48999542536499874 1106 98-99 0.4277359720127514 1106 100-101 0.6620046296893456 1107 1 0.09372982781087558 1107 2 0.0531733820395246 1107 3 -0.030011950461627634 1107 4 0.013876208172050042 1107 5 -0.05127106376017565 1107 6 0.019748371177541912 1107 7 0.017167367378348786 1107 8 -0.09539491431266356 1107 9 -0.18733524583735317 1107 10-11 -0.11768954570894863 1107 12-13 -0.14729917487172628 1107 14-15 0.04008809184649209 1107 16-17 0.0952680324590176 1107 18-19 -0.01615742164172218 1107 20-21 -0.12477528081247868 1107 22-23 -0.09431295415750895 1107 24-25 -0.11686039086504962 1107 26-27 -0.11174731912712588 1107 28-29 -0.11967793709954577 1107 30-31 -0.03296512189768208 1107 32-33 -0.0727773523397417 1107 34-35 -0.002682443864031825 1107 36-37 0.06425122004694828 1107 38-39 -0.16757260228477122 1107 40-41 -0.025183756956778325 1107 42-43 -0.035021431830152494 1107 44-45 -0.02899790457617968 1107 46-47 -0.2790399971157811 1107 48-49 -0.47265372407825623 1107 50-51 -0.32850155108449997 1107 52-53 0.1126582345166085 1107 54-55 -0.26505528427146885 1107 56-57 -0.3501708529608578 1107 58-59 -0.2804982187195151 1107 60-61 -0.2561303209660579 1107 62-63 -0.341984826912082 1107 64-65 -0.3867768401809464 1107 66-67 -0.2509518014778038 1107 68-69 -0.10975241111597711 1107 70-71 -0.28076619794353164 1107 72-73 -0.2569131390971222 1107 74-75 -0.22314590609601836 1107 76-77 -0.3559286317075312 1107 78-79 -0.3066891913472496 1107 80-81 -0.2399281190531255 1107 82-83 -0.2737544062995667 1107 84-85 0.012709938422236888 1107 86-87 -0.06761647274850446 1107 88-89 -0.18745290142081217 1107 90-91 -0.19512743679930367 1107 92-93 0.022218320187210594 1107 94-95 0.14587539413818362 1107 96-97 0.3153167197836346 1107 98-99 0.37356299496968504 1107 100-101 1.1569798587880946 1108 1 -0.028955787717272585 1108 2 0.036593128240454575 1108 3 -0.004917791420638196 1108 4 0.0959925315971688 1108 5 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692.0 10 1196.0 11 1854.0 12 2783.0 13 3723.0 14 4750.0 15 6357.0 16 8298.0 17 11248.0 18 9750.0 19 6037.0 20 5080.0 21 4908.0 22 5255.0 23 5870.0 24 6812.0 25 8172.0 26 9919.0 27 12242.0 28 14976.0 29 18273.0 30 22485.0 31 27470.0 32 34373.0 33 43369.0 34 56891.0 35 77971.0 36 109020.0 37 142352.0 38 86871.0 39 5079.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.97929837117146 19.648257855883795 15.158443004254304 21.214000768690443 2 15.938583378391272 21.29358693001707 39.51992091006436 23.247908781527304 3 19.554448885260847 26.176889967877703 30.265459518483574 24.003201628377877 4 12.996167932538652 17.659841735083823 36.87251882907283 32.47147150330469 5 13.103579871306966 38.22138064092099 33.446407937837236 15.228631549934804 6 30.54536733255243 36.74319317754186 17.518060539245564 15.193378950660144 7 27.7414122997175 34.30747173651489 20.39820640161975 17.552909562147867 8 27.19098324596785 33.893523646983375 19.325307015916596 19.590186091132182 9 27.271016743431687 16.747009344308346 20.343614015932495 35.63835989632747 10-11 24.97575144033794 27.293012704655194 26.372296219344786 21.35893963566208 12-13 26.57305499829066 24.95600772779914 27.39453117753532 21.07640609637488 14-15 23.255393324084416 25.854100330867396 26.85630920532197 24.034197139726217 16-17 21.360927222519624 29.244690492974545 27.42545224226299 21.968930042242846 18-19 21.23113596942798 26.70519970795453 31.27841447400676 20.78524984861073 20-21 22.3433251533092 25.531016955440954 31.528957815456536 20.59670007579331 22-23 21.84645738674384 25.830116751006717 31.406365321598606 20.91706054065084 24-25 21.78375320001484 26.06216641667241 31.39963216392556 20.754448219387186 26-27 21.554695869775937 26.12882345029639 31.373907881407533 20.942572798520146 28-29 21.15459152607842 26.355832090494154 31.53388329657268 20.955693086854748 30-31 21.280890985463845 26.11386433805504 31.457391972650296 21.147852703830814 32-33 21.238342912988024 26.33350449719236 31.255139056831922 21.173013532987692 34-35 21.728489453344206 26.210644248052628 31.24701442550093 20.813851873102234 36-37 21.94518077517572 25.91904786197603 31.04128170795456 21.094489654893696 38-39 21.696624374730845 26.199092324510552 31.127637724848444 20.97664557591016 40-41 21.630619498928358 26.396199726375 31.033381587621285 20.93979918707536 42-43 21.612634142689327 26.527737564325882 30.488628224741554 21.37100006824324 44-45 21.807584849382284 26.552604397519197 30.214509414973723 21.425301338124797 46-47 22.134687192751386 27.030921633217698 29.467113300701484 21.367277873329428 48-49 22.340815297579177 27.12760283642835 28.945124532667094 21.58645733332538 50-51 22.23012841153173 27.403227580321804 28.832436930472795 21.534207077673674 52-53 22.52914305022311 27.636721402709085 28.4713107830114 21.362824764056406 54-55 21.93226434370565 27.590506566454962 28.613007821972282 21.864221267867105 56-57 22.177111819536595 27.860757505779794 28.37449482717606 21.58763584750755 58-59 22.314897959859902 27.4862571365141 28.322591975294326 21.87625292833167 60-61 22.092426850942516 27.534753837202675 28.905888636631715 21.46693067522309 62-63 22.063302110335396 27.63904571068102 28.913543041618293 21.384109137365286 64-65 21.74258774664715 27.81141470118727 28.70327727523029 21.74272027693529 66-67 21.81899614861458 27.858906651772614 28.439231525043944 21.882865674568862 68-69 21.89761726193158 27.684853672692196 28.577949801107227 21.839579264269 70-71 21.9823713829136 27.85610363040327 28.334053283369688 21.827471703313446 72-73 22.000495572313145 27.704082376308993 28.294595214149513 22.000826837228345 74-75 21.9869546061371 28.211111442380638 27.96908592800188 21.832848023480384 76-77 21.981050007917364 27.924438546358978 28.194026399600887 21.90048504612278 78-79 22.037116915833295 27.98325769878832 27.95874190233304 22.02088348304534 80-81 22.108920167883472 28.33682980130432 27.73350685356315 21.82074317724906 82-83 22.027360587898126 28.41081303156507 27.744656601098 21.817169779438807 84-85 21.90141167098874 28.299626024158986 27.70787867621963 22.091083628632646 86-87 21.892627010551774 28.155974092663456 27.571236893107386 22.380162003677384 88-89 21.81922542604749 28.210799389164027 27.500145815879613 22.469829368908872 90-91 21.741542573147015 28.170366825437437 27.732697719984568 22.355392881430976 92-93 21.89094258845672 28.161279847309018 27.671992736574925 22.275784827659333 94-95 21.601691292692383 28.03556221233278 27.798502887855765 22.56424360711907 96-97 21.76884952396861 27.736890493888783 27.8255808789363 22.66867910320631 98-99 21.426630128559516 28.10007145877995 27.44805980794956 23.025238604710978 100-101 21.100644357233772 28.493185193042002 27.55621552821383 22.849954921510395 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 392.0 1 301.0 2 367.0 3 477.0 4 357.5 5 299.0 6 447.0 7 844.5 8 1301.5 9 1599.5 10 1554.0 11 1372.5 12 1341.5 13 1434.0 14 1584.0 15 1720.0 16 1823.0 17 1910.5 18 2051.0 19 2181.0 20 2262.0 21 2470.5 22 2808.5 23 3162.0 24 3500.5 25 4030.5 26 4947.5 27 6041.0 28 7014.0 29 8302.0 30 9936.5 31 11682.0 32 13510.5 33 14822.0 34 16397.5 35 17658.0 36 19022.5 37 20719.5 38 21211.0 39 21119.0 40 21280.5 41 22152.0 42 23204.5 43 24180.0 44 25170.5 45 26087.5 46 26305.5 47 26913.0 48 29730.0 49 29489.5 50 26868.0 51 25447.5 52 23665.5 53 22816.0 54 22253.0 55 21077.0 56 19270.0 57 17243.0 58 16006.5 59 14573.0 60 12265.0 61 9986.5 62 8342.5 63 6596.0 64 4730.0 65 3608.5 66 2768.0 67 2206.5 68 1673.5 69 1120.0 70 820.0 71 649.0 72 568.0 73 468.0 74 360.5 75 286.5 76 218.0 77 150.0 78 110.0 79 79.0 80 57.0 81 40.5 82 25.5 83 18.0 84 12.5 85 6.0 86 2.5 87 2.0 88 1.5 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02040589173746893 2 0.013250579050304499 3 0.00901039375420706 4 0.0 5 0.0026501158100608996 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.950347430182699E-4 14-15 1.32505790503045E-4 16-17 0.0 18-19 9.275405335213149E-4 20-21 0.0 22-23 1.32505790503045E-4 24-25 0.0 26-27 0.002053839752797197 28-29 0.0036439092388337374 30-31 0.0018550810670426299 32-33 0.006161519258391592 34-35 0.0017888281717911073 36-37 4.6377026676065747E-4 38-39 0.0011925521145274049 40-41 1.9875868575456747E-4 42-43 0.004041426610342872 44-45 0.0011262992192758825 46-47 2.6501158100609E-4 48-49 3.3126447625761245E-4 50-51 1.32505790503045E-4 52-53 1.9875868575456747E-4 54-55 0.001987586857545675 56-57 0.015304418803101696 58-59 0.026699916786363565 60-61 0.021598443851996333 62-63 0.014840648536341039 64-65 0.018484557775174774 66-67 0.0027163687053124224 68-69 7.950347430182699E-4 70-71 2.6501158100609E-4 72-73 1.32505790503045E-4 74-75 0.0015238165907850171 76-77 0.0017888281717911073 78-79 0.009076646649458581 80-81 0.014973154326844082 82-83 0.017424511451150416 84-85 0.029615044177430553 86-87 0.009474164020967716 88-89 0.04081178347493786 90-91 0.04597950930455661 92-93 0.02901876812016685 94-95 0.030675090501454914 96-97 0.04962341854339035 98-99 0.05339983357272713 100-101 0.05883257098335198 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 754684.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.362558570295505 #Duplication Level Percentage of deduplicated Percentage of total 1 83.63354787785309 52.992355758520794 2 9.817653625455842 12.441433057316395 3 2.708773927329725 5.149045398723572 4 1.148521887834952 2.91093141548834 5 0.6669995270940748 2.113139830092886 6 0.39030219849408637 1.4838327547317989 7 0.27914533291561 1.2381153754542982 8 0.192621949227812 0.9764015631897379 9 0.1604426874914263 0.9149453265016005 >10 0.900831568501331 10.62284491198599 >50 0.06604667470033769 2.8622657041462545 >100 0.033022698870892916 3.818767341158851 >500 0.001045022115443512 0.43225331518070087 >1k 8.360176923548095E-4 1.3494673044397494 >5k 2.0900442308870239E-4 0.6942009430690056 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5242 0.6945953538169618 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4749 0.6292699990989606 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2758 0.3654509702073981 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1436 0.1902783151623726 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1247 0.1652347207572971 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 761 0.10083690657281723 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 1.32505790503045E-4 5 0.0 0.0 0.0 0.0 1.32505790503045E-4 6 1.32505790503045E-4 0.0 0.0 0.0 1.32505790503045E-4 7 1.32505790503045E-4 0.0 0.0 0.0 1.32505790503045E-4 8 2.6501158100609E-4 0.0 0.0 0.0 1.32505790503045E-4 9 2.6501158100609E-4 0.0 0.0 0.0 1.32505790503045E-4 10-11 2.6501158100609E-4 0.0 0.0 1.32505790503045E-4 1.32505790503045E-4 12-13 2.6501158100609E-4 0.0 0.0 1.32505790503045E-4 1.32505790503045E-4 14-15 2.6501158100609E-4 0.0 0.0 1.32505790503045E-4 1.32505790503045E-4 16-17 3.3126447625761245E-4 0.0 0.0 1.32505790503045E-4 1.32505790503045E-4 18-19 3.9751737150913493E-4 0.0 0.0 1.987586857545675E-4 1.32505790503045E-4 20-21 3.9751737150913493E-4 0.0 0.0 2.6501158100609E-4 1.32505790503045E-4 22-23 3.9751737150913493E-4 0.0 0.0 3.3126447625761245E-4 1.32505790503045E-4 24-25 3.9751737150913493E-4 0.0 0.0 6.625289525152249E-4 1.32505790503045E-4 26-27 3.9751737150913493E-4 0.0 0.0 9.937934287728373E-4 1.32505790503045E-4 28-29 3.9751737150913493E-4 0.0 0.0 0.002186345543300242 1.32505790503045E-4 30-31 3.9751737150913493E-4 6.62528952515225E-5 0.0 0.004703955562858097 1.32505790503045E-4 32-33 3.9751737150913493E-4 1.32505790503045E-4 0.0 0.0072215655824159515 1.32505790503045E-4 34-35 3.9751737150913493E-4 1.32505790503045E-4 0.0 0.010732969030746643 1.32505790503045E-4 36-37 3.9751737150913493E-4 1.32505790503045E-4 0.0 0.013979360898071246 1.32505790503045E-4 38-39 3.9751737150913493E-4 1.32505790503045E-4 0.0 0.019080833832438478 1.32505790503045E-4 40-41 3.9751737150913493E-4 1.32505790503045E-4 0.0 0.02424855966205723 1.32505790503045E-4 42-43 3.9751737150913493E-4 1.32505790503045E-4 0.0 0.0329939418352582 1.32505790503045E-4 44-45 3.9751737150913493E-4 1.32505790503045E-4 0.0 0.04723831431433553 1.32505790503045E-4 46-47 5.3002316201218E-4 1.32505790503045E-4 0.0 0.05850130650709436 1.32505790503045E-4 48-49 5.3002316201218E-4 1.32505790503045E-4 0.0 0.07294443767192627 1.32505790503045E-4 50-51 5.3002316201218E-4 1.32505790503045E-4 0.0 0.09096522518034038 1.32505790503045E-4 52-53 5.3002316201218E-4 1.32505790503045E-4 0.0 0.1213753041007892 1.32505790503045E-4 54-55 6.625289525152249E-4 1.32505790503045E-4 0.0 0.14297374795278553 1.32505790503045E-4 56-57 6.625289525152249E-4 1.32505790503045E-4 0.0 0.19345845413444568 1.32505790503045E-4 58-59 6.625289525152249E-4 1.32505790503045E-4 0.0 0.2597776022812197 1.32505790503045E-4 60-61 6.625289525152249E-4 1.32505790503045E-4 0.0 0.3190076906360808 1.32505790503045E-4 62-63 6.625289525152249E-4 1.32505790503045E-4 0.0 0.3981799004616502 1.32505790503045E-4 64-65 6.625289525152249E-4 1.32505790503045E-4 0.0 0.45687996565449907 1.32505790503045E-4 66-67 6.625289525152249E-4 1.32505790503045E-4 0.0 0.5604332409326287 1.32505790503045E-4 68-69 6.625289525152249E-4 1.32505790503045E-4 0.0 0.6818085450334179 1.32505790503045E-4 70-71 6.625289525152249E-4 1.32505790503045E-4 0.0 0.7656184575265939 1.32505790503045E-4 72-73 6.625289525152249E-4 1.32505790503045E-4 0.0 0.9160125297475499 1.32505790503045E-4 74-75 6.625289525152249E-4 1.32505790503045E-4 0.0 1.0564024147855262 1.32505790503045E-4 76-77 6.625289525152249E-4 1.32505790503045E-4 0.0 1.1443200067842965 1.32505790503045E-4 78-79 6.625289525152249E-4 1.32505790503045E-4 0.0 1.2437656025568318 1.32505790503045E-4 80-81 6.625289525152249E-4 1.32505790503045E-4 0.0 1.4333151358714376 1.987586857545675E-4 82-83 6.625289525152249E-4 1.32505790503045E-4 0.0 1.5897382215602822 2.6501158100609E-4 84-85 6.625289525152249E-4 1.32505790503045E-4 0.0 1.8289111734182784 2.6501158100609E-4 86-87 6.625289525152249E-4 1.32505790503045E-4 0.0 2.187471842519518 2.6501158100609E-4 88-89 6.625289525152249E-4 1.32505790503045E-4 0.0 2.6536272135092305 2.6501158100609E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 825 0.0 32.244267 2 GGTATCA 4885 0.0 31.514763 1 GTACCTG 905 0.0 30.451803 1 TCGTTAG 80 4.4976667E-4 29.68133 9 GTATCAA 6910 0.0 28.192879 1 ACGTCTA 170 1.1232987E-7 25.143497 5 GTACCCG 115 1.2783812E-4 24.7906 1 GTACATG 4735 0.0 23.782745 1 GTAAGGT 320 0.0 23.746637 4 GAGTACT 4820 0.0 23.745064 12-13 CGTCTAT 180 1.9543586E-7 23.74349 6 ATCAACG 8295 0.0 23.41897 3 TATCAAC 8300 0.0 23.407965 2 TCAACGC 8365 0.0 23.221458 4 CAACGCA 8495 0.0 22.866096 5 CTACGTC 210 3.444984E-8 22.617344 3 AACGCAG 8630 0.0 22.560444 6 TACATGG 4890 0.0 22.34279 2 GTACTTT 5205 0.0 21.897463 14-15 GTCTATC 175 4.0558243E-6 21.708334 7 >>END_MODULE