##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936635_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 327609 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.65544597370646 31.0 31.0 34.0 30.0 34.0 2 31.53063560524894 31.0 31.0 34.0 30.0 34.0 3 31.479492321639515 31.0 31.0 34.0 28.0 34.0 4 35.183239776685014 37.0 35.0 37.0 33.0 37.0 5 35.25557295434496 37.0 35.0 37.0 33.0 37.0 6 35.3784267220986 37.0 35.0 37.0 33.0 37.0 7 35.36912905323114 37.0 35.0 37.0 33.0 37.0 8 35.3529084976298 37.0 35.0 37.0 33.0 37.0 9 36.81696168298185 39.0 37.0 39.0 33.0 39.0 10-11 36.67439539206798 39.0 37.0 39.0 32.0 39.0 12-13 36.53782863108157 39.0 37.0 39.0 32.0 39.0 14-15 37.303711131257074 39.0 36.5 41.0 32.0 41.0 16-17 37.31583991892775 39.0 36.5 41.0 32.0 41.0 18-19 37.6540082842657 39.5 37.0 41.0 32.0 41.0 20-21 37.69619271753828 40.0 37.0 41.0 32.5 41.0 22-23 37.65908140496751 40.0 36.5 41.0 33.0 41.0 24-25 37.62955840651509 40.0 36.0 41.0 33.0 41.0 26-27 37.33274574263833 39.5 35.5 41.0 32.5 41.0 28-29 37.09840999484141 39.0 35.0 41.0 32.0 41.0 30-31 36.62467453580335 38.5 35.0 40.0 31.5 41.0 32-33 36.085811134614744 38.0 35.0 40.0 31.0 41.0 34-35 35.72645440143586 38.0 35.0 40.0 30.0 41.0 36-37 35.28013271918659 38.0 35.0 40.0 30.0 41.0 38-39 34.82656306755919 37.0 35.0 40.0 28.0 41.0 40-41 34.37894868578091 36.5 34.5 40.0 25.5 41.0 42-43 33.96105723591232 36.5 34.0 40.0 23.5 41.0 44-45 33.25209319646286 36.0 33.0 40.0 17.5 41.0 46-47 32.025718157926065 36.0 32.5 40.0 2.0 41.0 48-49 31.279571074054743 36.0 31.0 40.0 2.0 41.0 50-51 30.1203339957083 35.5 29.0 39.0 2.0 40.0 52-53 30.04261482437906 35.0 29.0 39.0 2.0 40.0 54-55 30.39977992057605 35.0 30.5 39.5 2.0 41.0 56-57 30.344769221846775 35.0 30.5 40.0 2.0 41.0 58-59 30.154696604794132 35.0 29.5 40.0 2.0 41.0 60-61 29.760516347230997 35.0 28.5 39.0 2.0 41.0 62-63 29.25162617632605 35.0 28.0 39.0 2.0 40.5 64-65 28.66349825554243 35.0 26.0 38.0 2.0 40.0 66-67 28.18488808305022 35.0 26.0 37.5 2.0 40.0 68-69 27.90738349679038 35.0 26.0 37.0 2.0 39.0 70-71 27.421839754097107 34.5 24.5 36.5 2.0 39.0 72-73 26.827861871926594 34.0 22.5 35.5 2.0 39.0 74-75 26.391984041952448 34.0 20.0 35.0 2.0 37.5 76-77 25.817628636575918 33.0 15.5 35.0 2.0 37.0 78-79 25.306262953703957 33.0 9.5 35.0 2.0 36.0 80-81 24.889677939250753 33.0 4.0 35.0 2.0 36.0 82-83 24.465359620767437 33.0 2.0 35.0 2.0 36.0 84-85 24.13314499906901 33.0 2.0 35.0 2.0 35.0 86-87 23.58146754210049 32.0 2.0 35.0 2.0 35.0 88-89 22.930479626628085 31.5 2.0 35.0 2.0 35.0 90-91 22.316036799965815 31.0 2.0 35.0 2.0 35.0 92-93 21.90274076719504 31.0 2.0 35.0 2.0 35.0 94-95 21.486766236580802 31.0 2.0 35.0 2.0 35.0 96-97 20.702053362392363 29.5 2.0 34.0 2.0 35.0 98-99 19.892066457270708 29.0 2.0 34.0 2.0 35.0 100-101 17.74051842287605 23.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3697322047403624 1101 2 0.14756550628844423 1101 3 0.4177874604632663 1101 4 0.4442821173060949 1101 5 0.4849427151828891 1101 6 0.6300276910509766 1101 7 0.33469101247230526 1101 8 0.4698510918379313 1101 9 0.6705305610283858 1101 10-11 0.666065922783865 1101 12-13 0.6840391622542086 1101 14-15 0.6139146504268354 1101 16-17 0.9357311268776485 1101 18-19 0.5579023482197201 1101 20-21 0.7119168501905975 1101 22-23 0.6533164549653634 1101 24-25 0.6696302330720485 1101 26-27 0.8696440937860288 1101 28-29 0.7152803722601604 1101 30-31 0.46924776073072394 1101 32-33 0.5588175991427704 1101 34-35 0.46177405585099507 1101 36-37 0.8148310505719891 1101 38-39 1.0086670210124424 1101 40-41 1.1184147855246636 1101 42-43 1.1732747990317662 1101 44-45 0.8723471446648716 1101 46-47 1.2287141565900868 1101 48-49 1.3916501108796346 1101 50-51 1.0524401220169004 1101 52-53 1.4085159160404679 1101 54-55 1.464645816707261 1101 56-57 1.8398127786502023 1101 58-59 2.087357763734204 1101 60-61 1.8926819958313068 1101 62-63 1.5930344110664407 1101 64-65 1.4765489941547436 1101 66-67 1.618752243715008 1101 68-69 1.6466750552070621 1101 70-71 1.2653826505410244 1101 72-73 1.0935843961556202 1101 74-75 1.2397141635398192 1101 76-77 1.1915857944280823 1101 78-79 1.4594347263137557 1101 80-81 1.5637351669847774 1101 82-83 1.8980159671296484 1101 84-85 1.520766541757844 1101 86-87 1.5820320565543113 1101 88-89 1.921068764159017 1101 90-91 1.7139958220700073 1101 92-93 1.9623850337083262 1101 94-95 2.1786097368733657 1101 96-97 2.338973976393852 1101 98-99 1.733602690282769 1101 100-101 1.5792568564085379 1102 1 0.3521624691920522 1102 2 0.40689376794553667 1102 3 0.42536909892520214 1102 4 0.2195758087618671 1102 5 -0.016169948482172458 1102 6 -0.0693461121886827 1102 7 0.02205839848897284 1102 8 0.20527002264577732 1102 9 0.39101909800504586 1102 10-11 0.25554478217422627 1102 12-13 0.4362774275339305 1102 14-15 0.24689089635138117 1102 16-17 -0.07425697020872946 1102 18-19 0.19073627669873616 1102 20-21 0.009981332931374709 1102 22-23 0.0585071603423728 1102 24-25 0.11737385466289396 1102 26-27 -0.20257554084627571 1102 28-29 0.055600198374122556 1102 30-31 0.04655200955337335 1102 32-33 0.1468215322794464 1102 34-35 -0.2893903596124403 1102 36-37 -0.8058929570872806 1102 38-39 -0.621398186253515 1102 40-41 -0.5839218081719721 1102 42-43 -0.7765130185948976 1102 44-45 -0.7514819708968972 1102 46-47 -1.7259253921342363 1102 48-49 -1.2773324487643087 1102 50-51 -1.1547870166261696 1102 52-53 -1.1277103428220236 1102 54-55 -0.9234797331555953 1102 56-57 -1.1945504154707969 1102 58-59 -1.095274332731151 1102 60-61 -1.4278686433034018 1102 62-63 -1.1269754217752492 1102 64-65 -1.342759601818969 1102 66-67 -1.2345184699422909 1102 68-69 -1.4300081720350875 1102 70-71 -1.4813958000101337 1102 72-73 -1.5980525132269783 1102 74-75 -1.4352586667660354 1102 76-77 -1.4711218596628974 1102 78-79 -1.6569421302820082 1102 80-81 -1.3415590419889831 1102 82-83 -1.1480172575249092 1102 84-85 -1.386545987352541 1102 86-87 -1.4106838778244075 1102 88-89 -1.0954788733713876 1102 90-91 -0.8259565662754866 1102 92-93 -1.101360243940075 1102 94-95 -0.890233092914972 1102 96-97 -0.5184627570326299 1102 98-99 -0.4051180052614036 1102 100-101 -0.5516105037322276 1103 1 0.4488623216994263 1103 2 0.31127496934001186 1103 3 0.21447140517352636 1103 4 0.4781404373486211 1103 5 0.26597673963797064 1103 6 0.6109720025873955 1103 7 0.3752445744074464 1103 8 0.428117971157441 1103 9 0.050780428120361876 1103 10-11 0.3243676904606332 1103 12-13 0.2260962462520837 1103 14-15 0.41477689125617445 1103 16-17 0.8730982188499965 1103 18-19 0.6636722179698538 1103 20-21 0.5798727247254192 1103 22-23 0.5088122019084764 1103 24-25 0.44486614482111264 1103 26-27 0.509629473902983 1103 28-29 0.6743862335041797 1103 30-31 0.24712521952923083 1103 32-33 0.12242239712256264 1103 34-35 0.17289845776487311 1103 36-37 0.15361361303875753 1103 38-39 0.375069532974166 1103 40-41 0.33661653991062224 1103 42-43 0.40744213023857156 1103 44-45 0.20026447587167695 1103 46-47 0.26775588971076303 1103 48-49 0.6978535930699614 1103 50-51 0.7219476977744748 1103 52-53 1.1199808947751855 1103 54-55 0.8948026481798834 1103 56-57 0.5282514415954402 1103 58-59 0.2863376946220235 1103 60-61 0.37390043017474284 1103 62-63 0.4522515827836102 1103 64-65 0.4741400361536776 1103 66-67 0.5819034930504685 1103 68-69 0.8852698850847887 1103 70-71 0.5689611991853631 1103 72-73 0.42753242911681255 1103 74-75 0.5334242911315243 1103 76-77 0.5581378279209055 1103 78-79 0.49401573375660845 1103 80-81 0.7407686598077454 1103 82-83 1.0060203530945593 1103 84-85 1.0395065736557711 1103 86-87 0.5008833989253922 1103 88-89 0.08877377479165816 1103 90-91 0.3422520687473103 1103 92-93 0.14145368070460407 1103 94-95 0.7097434423491755 1103 96-97 0.9400256111625929 1103 98-99 0.7395337110164242 1103 100-101 0.3985185523149717 1104 1 0.1574379030947739 1104 2 0.24851069872690346 1104 3 0.1889692910086218 1104 4 0.025048471175260545 1104 5 0.2889682829783453 1104 6 0.36566280597006084 1104 7 0.3191531367753129 1104 8 0.5525894299312242 1104 9 0.17102355708441763 1104 10-11 0.6455555868665499 1104 12-13 0.45194851897935706 1104 14-15 0.10455622529423181 1104 16-17 0.08279695035105306 1104 18-19 0.13826920528486397 1104 20-21 0.11977758730469645 1104 22-23 -0.028582089846274528 1104 24-25 -0.003402882663031903 1104 26-27 -0.44531238656213645 1104 28-29 -0.5712863352061817 1104 30-31 -0.10091917793376837 1104 32-33 -0.2688632794102048 1104 34-35 -0.479321415385229 1104 36-37 -0.2579878668766753 1104 38-39 -0.26344130212097383 1104 40-41 -0.6532090414847289 1104 42-43 -0.9430467703956751 1104 44-45 -0.9838000061579208 1104 46-47 -0.6978889305852185 1104 48-49 -0.1901617346255975 1104 50-51 -0.30850949038620357 1104 52-53 0.0951064233164125 1104 54-55 -0.36418255266578825 1104 56-57 -0.6012741926540279 1104 58-59 -0.31349052947945566 1104 60-61 -0.1355802780704991 1104 62-63 -0.45816067937283833 1104 64-65 -0.33063004841291743 1104 66-67 -0.07163773316517563 1104 68-69 -0.2007171656551705 1104 70-71 -0.41115243717827354 1104 72-73 -0.41132856454069255 1104 74-75 -0.36093353127862216 1104 76-77 -0.5043291382524551 1104 78-79 -0.1593603233258456 1104 80-81 -0.03288356006540383 1104 82-83 -0.29267150737056014 1104 84-85 -0.6573750331095525 1104 86-87 -0.5119006919837013 1104 88-89 -0.9178660243626737 1104 90-91 -1.1085935972146395 1104 92-93 -1.023352080394762 1104 94-95 -0.980134993168793 1104 96-97 -0.3903456890488215 1104 98-99 -0.4436718915205766 1104 100-101 -0.134184936057121 1105 1 -0.06433212274501798 1105 2 -0.042639172074128595 1105 3 0.052728980931103564 1105 4 0.033317205025390706 1105 5 -0.017104068442840514 1105 6 0.24537856824395732 1105 7 -0.13782360741073063 1105 8 0.5758452438847144 1105 9 0.39421477155470086 1105 10-11 0.34523258945053215 1105 12-13 0.3588929634238056 1105 14-15 0.24870997206425471 1105 16-17 0.20466387541565467 1105 18-19 0.19072398564662052 1105 20-21 0.05979696714965854 1105 22-23 -0.11101103041475113 1105 24-25 0.02876765997263675 1105 26-27 -0.17925941498590703 1105 28-29 -0.22002664528369564 1105 30-31 -0.1786323562283414 1105 32-33 -0.14118240085723244 1105 34-35 -0.08303588566946729 1105 36-37 0.014977249402399195 1105 38-39 -0.4161867801571475 1105 40-41 -0.4272723489782635 1105 42-43 -0.32292403137759607 1105 44-45 -0.5530183524111507 1105 46-47 -0.04007239311751931 1105 48-49 -0.5077650937987244 1105 50-51 -0.47950432242754815 1105 52-53 -0.5409723880530954 1105 54-55 -0.48133371545648274 1105 56-57 -0.29936850789950853 1105 58-59 0.2025624420967702 1105 60-61 -0.1573180041686939 1105 62-63 -0.41113225560022926 1105 64-65 -0.77630188303824 1105 66-67 -0.42276822412124915 1105 68-69 -0.7172869330970322 1105 70-71 -1.1097635482893864 1105 72-73 -1.2764448436104594 1105 74-75 -1.2189115674543345 1105 76-77 -0.8918937377356606 1105 78-79 -1.0594249228090504 1105 80-81 -0.888019218980137 1105 82-83 -1.004396313572105 1105 84-85 -1.080695446546244 1105 86-87 -1.646622914205924 1105 88-89 -1.4257148615719757 1105 90-91 -0.7908287393335023 1105 92-93 -1.039954147578225 1105 94-95 -1.1893727192669878 1105 96-97 -1.3748418130798328 1105 98-99 -0.9979031576704465 1105 100-101 -0.6882870032405819 1106 1 -0.049579760412012064 1106 2 0.04386294587960293 1106 3 0.006810114850946292 1106 4 0.017913164621347732 1106 5 -0.08482971197493328 1106 6 0.0639595392442871 1106 7 0.14311480901155704 1106 8 0.2844229564946872 1106 9 0.5045927448359038 1106 10-11 0.341367323891717 1106 12-13 0.3340966128209999 1106 14-15 0.2953212460948933 1106 16-17 0.39674191386465907 1106 18-19 0.3810017634244005 1106 20-21 -0.22987800841241324 1106 22-23 0.12776381481170063 1106 24-25 0.23491379878006313 1106 26-27 0.18539926862438705 1106 28-29 0.30117325696458863 1106 30-31 0.2934961872711668 1106 32-33 -0.17252811607392005 1106 34-35 -0.3150047680415753 1106 36-37 -0.11031600572956535 1106 38-39 -0.12534285705515913 1106 40-41 -0.46085413457618074 1106 42-43 -0.20793543574383477 1106 44-45 -0.08804197574122696 1106 46-47 -0.6756092129285314 1106 48-49 -0.46004596704733913 1106 50-51 -0.8930632992929723 1106 52-53 -0.8820810553714402 1106 54-55 -0.8496988180957814 1106 56-57 -0.7478207724808037 1106 58-59 -0.7815691968735763 1106 60-61 -0.43837046425340986 1106 62-63 -0.59375281604337 1106 64-65 -0.836985330415235 1106 66-67 -0.8262709937530488 1106 68-69 -0.8357253495193184 1106 70-71 -1.0539310295536382 1106 72-73 -1.0168407380386135 1106 74-75 -0.6574664713318192 1106 76-77 -0.9346682571230822 1106 78-79 -1.3010264216686132 1106 80-81 -0.685917557201055 1106 82-83 -0.7345404973453249 1106 84-85 -0.11225295713601469 1106 86-87 -0.3236675541537899 1106 88-89 -0.9915506386980795 1106 90-91 -1.3109737377043054 1106 92-93 -0.857859002579854 1106 94-95 -0.6144867629294204 1106 96-97 -0.17145716009840228 1106 98-99 -0.04354913355835777 1106 100-101 -0.2954880459254987 1107 1 0.3003572784717612 1107 2 0.30552765469500187 1107 3 0.3276329194517551 1107 4 0.5738471441864519 1107 5 0.4603374941572227 1107 6 0.45465650612420205 1107 7 0.5901302830791835 1107 8 0.6864360219827006 1107 9 0.727948361051979 1107 10-11 0.669267972095426 1107 12-13 0.7305868475096204 1107 14-15 0.5467727322307638 1107 16-17 0.6979115539299059 1107 18-19 0.37811991904400344 1107 20-21 0.3639636338163257 1107 22-23 0.5094180441929907 1107 24-25 0.49871242462200627 1107 26-27 0.21586146237560655 1107 28-29 0.4503023652830649 1107 30-31 0.4855487196602226 1107 32-33 0.4044788223256006 1107 34-35 0.9139581905393044 1107 36-37 0.882196269575303 1107 38-39 0.8775051826990818 1107 40-41 0.8681367128212827 1107 42-43 0.8849850304219586 1107 44-45 1.4903974336919532 1107 46-47 1.0539918079711725 1107 48-49 1.0721740672643527 1107 50-51 1.4474929558305405 1107 52-53 1.187962130038482 1107 54-55 1.3809501462149854 1107 56-57 0.9505194203745866 1107 58-59 1.1926040687378219 1107 60-61 1.2311386080631337 1107 62-63 1.241369345424431 1107 64-65 1.473886237358812 1107 66-67 1.3860854422252125 1107 68-69 1.5457894357790494 1107 70-71 1.4164772458188466 1107 72-73 1.6880364244010657 1107 74-75 1.6826334213384904 1107 76-77 1.232689995853839 1107 78-79 1.7291357560254035 1107 80-81 1.6362363045549309 1107 82-83 1.6674936960340396 1107 84-85 1.536035261106651 1107 86-87 1.7339566567194673 1107 88-89 1.9312015649409915 1107 90-91 2.529768442863105 1107 92-93 2.452344923827475 1107 94-95 1.918464296423057 1107 96-97 1.66832581420163 1107 98-99 1.3504437843724624 1107 100-101 1.1396028462630987 1108 1 0.36370831023810624 1108 2 0.3382188349300108 1108 3 0.3776812254392219 1108 4 0.5638886790782394 1108 5 0.45069281153168816 1108 6 0.15783479237639852 1108 7 0.24956894273253027 1108 8 0.2853724645150848 1108 9 0.5361281749294236 1108 10-11 0.5071993197911837 1108 12-13 0.3210387768590195 1108 14-15 0.56945177403815 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64-65 -2.3265796608160194 2316 66-67 -2.1816571130101394 2316 68-69 -2.1347869330970326 2316 70-71 -1.9669857705116058 2316 72-73 -1.6497781769437943 2316 74-75 -1.8916893452321126 2316 76-77 -2.127449293291214 2316 78-79 -2.604980478364606 2316 80-81 -2.1796858856468013 2316 82-83 -2.306896313572107 2316 84-85 -1.9212510021018012 2316 86-87 -1.3727340253170333 2316 88-89 -2.234048194905313 2316 90-91 -1.881384294889056 2316 92-93 -2.016065258689334 2316 94-95 -2.463817163711429 2316 96-97 -2.3873418130798285 2316 98-99 -2.52262537989267 2316 100-101 -2.9243981143516944 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 632.0 3 207.0 4 180.0 5 212.0 6 237.0 7 309.0 8 396.0 9 568.0 10 923.0 11 1624.0 12 2905.0 13 4108.0 14 4438.0 15 6031.0 16 8735.0 17 12485.0 18 8422.0 19 4175.0 20 3639.0 21 3582.0 22 3968.0 23 4327.0 24 4636.0 25 5046.0 26 5713.0 27 6422.0 28 7390.0 29 8637.0 30 10133.0 31 11872.0 32 14518.0 33 17962.0 34 24177.0 35 35706.0 36 37451.0 37 37741.0 38 24436.0 39 3650.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 57.99788750022895 15.757966138948756 12.76963373161484 13.474512629207446 2 14.404989406973693 23.764096051579184 42.02103952084109 19.80987502060603 3 23.342736248236953 36.71807740919166 28.629738855408814 11.30944748716258 4 10.445460068569894 17.051494585542926 38.29594961364559 34.20709573224159 5 9.785997148257048 43.705250687746364 30.36007071345479 16.1486814505418 6 21.20708714797872 36.38454068513983 22.32598193400231 20.08239023287914 7 23.066755800572043 41.7790761209657 19.492852498649256 15.661315579813001 8 25.582900565727247 27.686746472982172 21.069600021981845 25.660752939308733 9 31.296831203092463 18.862860527746054 21.509834934321816 28.330473334839667 10-11 23.25693949308608 27.725452416322256 24.583844160840815 24.43376392975085 12-13 33.35638543107422 26.600746826901645 24.957025393946612 15.085842348077517 14-15 28.060807014972767 25.006564324271512 32.770022715615156 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16.43750314785053 27.1258483934134 42.00110193846543 14.43554652027063 42-43 15.388137541151997 30.663483889479892 38.51277409862438 15.435604470743735 44-45 15.792036141084537 31.6588574655454 35.45748691259291 17.091619480777158 46-47 15.878042712827737 29.94583440804746 32.56116713190978 21.614955747215028 48-49 17.90580758594165 30.063857194312682 32.659963492732125 19.37037172701355 50-51 20.740073717386164 31.05547118840668 32.309735121068975 15.894719973138177 52-53 16.739723685736788 31.537585699546394 34.19907935945885 17.52361125525797 54-55 14.803062355192909 31.263068887728906 35.19785342088152 18.736015336196658 56-57 17.410441747454275 34.05045053844839 30.3680007814217 18.171106932675638 58-59 16.245491772628135 31.37375208145411 31.952667237492577 20.428088908425178 60-61 16.766000051892473 29.31802607138878 33.53245797854399 20.38351589817476 62-63 21.825879523147435 29.053758090880372 31.36262558893062 17.757736797041574 64-65 19.902413264714323 30.28348076653053 33.87407434540271 15.940031623352441 66-67 16.870116330365413 30.982252025210766 34.5541330785188 17.593498565905023 68-69 15.929602130196669 34.56347915836467 32.749178197852395 16.757740513586263 70-71 16.19126581679317 33.600846514312416 29.96147626965695 20.246411399237466 72-73 16.009610100985434 32.00639859328636 29.32225587046512 22.661735435263086 74-75 16.93078723245103 32.22647454382529 31.37770640255119 19.46503182117249 76-77 18.753367924686717 29.785811438960614 32.57586512730565 18.88495550904703 78-79 16.06574348497272 29.96523181083173 35.238330821533985 18.730693882661562 80-81 15.183041373306658 34.48554454235839 32.66872336851108 17.662690715823878 82-83 14.798369919549515 33.66079485507766 30.372386962410125 21.1684482629627 84-85 15.894565398241841 30.57331743795383 30.68642700464192 22.84569015916241 86-87 17.995667777453818 30.27884675564158 32.12836079262271 19.597124674281893 88-89 19.692135785093438 27.28402336153285 33.0926554051785 19.931185448195222 90-91 16.2102495751863 28.945652854305315 35.28648397769136 19.55761359281702 92-93 14.741240209016787 32.12129446300913 33.26407833091882 19.873386997055263 94-95 14.379215656944167 33.0360972422983 31.697466370142884 20.887220730614654 96-97 14.476451858115844 30.528631133552935 29.708636201280363 25.286280807050858 98-99 16.292204312157317 29.975310677788315 30.43596426537433 23.296520744680038 100-101 18.819952322848536 30.087459015517215 31.12285647528103 19.969732186353216 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1106.0 1 885.5 2 2240.5 3 3152.5 4 1918.5 5 1543.5 6 2249.0 7 3822.5 8 5368.5 9 5467.0 10 4152.0 11 2960.0 12 2735.5 13 2804.0 14 2758.0 15 2537.0 16 2343.5 17 2227.5 18 2236.5 19 2291.0 20 2338.0 21 2449.5 22 2522.5 23 2709.0 24 2991.0 25 3267.0 26 3524.5 27 3800.5 28 4170.5 29 4542.5 30 4789.0 31 5114.0 32 5537.0 33 5802.5 34 6158.5 35 6403.5 36 6630.5 37 6960.0 38 6847.5 39 6461.0 40 6149.0 41 5861.5 42 5635.0 43 5669.0 44 7385.0 45 8165.5 46 8514.5 47 13615.0 48 33723.0 49 32486.5 50 13560.5 51 9131.5 52 4896.5 53 3231.0 54 2603.0 55 2246.0 56 2101.5 57 1986.0 58 1893.0 59 1952.5 60 2116.0 61 2204.0 62 1876.5 63 1035.0 64 437.5 65 314.0 66 241.5 67 181.5 68 147.5 69 125.5 70 129.0 71 112.5 72 99.5 73 136.0 74 129.5 75 82.5 76 51.0 77 29.5 78 25.5 79 21.5 80 12.0 81 7.0 82 7.0 83 4.5 84 3.0 85 3.0 86 1.0 87 0.5 88 0.5 89 1.0 90 0.5 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010683467181915027 2 0.010683467181915027 3 0.015567337893647611 4 0.01831451516899719 5 0.027471772753495785 6 0.043649594486109965 7 0.0036629030337994375 8 0.02014596668589691 9 0.03143991770677851 10-11 0.03586592553928616 12-13 0.027471772753495785 14-15 0.024724595478146205 16-17 0.02686128891452921 18-19 0.022435281082021554 20-21 0.0462441508017179 22-23 0.012209676779331458 24-25 0.002289314396124649 26-27 7.631047987082161E-4 28-29 0.0 30-31 0.002594556315607935 32-33 0.0021366934363830055 34-35 0.0 36-37 1.5262095974164324E-4 38-39 0.0 40-41 0.001373588637674789 42-43 0.003815523993541081 44-45 0.001220967677933146 46-47 7.631047987082161E-4 48-49 9.157257584498594E-4 50-51 0.001984072476641362 52-53 0.0033576611143161512 54-55 0.005494354550699156 56-57 3.052419194832865E-4 58-59 0.004120765913024367 60-61 0.002594556315607935 62-63 7.631047987082161E-4 64-65 9.157257584498594E-4 66-67 0.015414716933905967 68-69 0.03891834473411903 70-71 0.04532842504326804 72-73 0.0112939510208816 74-75 0.02304576492098813 76-77 0.021519555323571696 78-79 0.047159876560167756 80-81 0.06837418996425618 82-83 0.042886489687401746 84-85 0.015414716933905967 86-87 0.019382861887188692 88-89 0.01892499900796376 90-91 0.03433971594186973 92-93 0.02304576492098813 94-95 0.01083608814165667 96-97 0.014041128296231178 98-99 0.04258124776791847 100-101 0.06059052101743237 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 327609.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.41505427482223 #Duplication Level Percentage of deduplicated Percentage of total 1 85.6033072013464 37.16472228250734 2 8.412650563587993 7.3047136162657305 3 2.434089445353422 3.170283761393722 4 1.0100953077940042 1.754133704424798 5 0.599276106829324 1.3008802351799633 6 0.3181551591829036 0.8287634102244281 7 0.24190882359575774 0.7351739294177754 8 0.17293866705961683 0.600651329328694 9 0.15042678087909936 0.58777081706276 >10 0.8448786415390094 6.9143465027571684 >50 0.08926724986661863 2.667715946208351 >100 0.09278170852278472 8.255515842229984 >500 0.0119491594309647 3.6652866883903577 >1k 0.01476072635589757 13.786860985329113 >5k 0.0035144586561660883 11.263180949279802 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8875 2.7090220354141676 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8570 2.615923249971765 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6932 2.115936985858142 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6761 2.0637406176265 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5771 1.7615511173380463 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4927 1.5039269372941524 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4706 1.4364684730883461 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3365 1.027139059061259 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3222 0.9834894645751491 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3175 0.9691430943594346 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2979 0.9093156781407103 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2455 0.7493689123314683 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2084 0.636124160203169 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1872 0.5714128732727123 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1729 0.5277632787866023 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1557 0.47526166863547703 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1499 0.45755763730544646 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1391 0.4245915100012515 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1351 0.41238183322192007 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1350 0.41207659130243673 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1340 0.4090241721076039 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1288 0.393151592294473 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1286 0.39254110845550644 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1234 0.3766685286423755 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1213 0.3702584483332265 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1156 0.3528596589226792 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 963 0.2939479684624049 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 941 0.2872326462337726 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 900 0.2747177275349578 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 777 0.2371729714385136 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 735 0.22435281082021555 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 731 0.2231318431422824 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 720 0.21977418202796628 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 710 0.21672176283313338 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 708 0.21611127899416682 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 706 0.21550079515520026 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 697 0.21275361787985064 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 672 0.2051225698927685 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 588 0.17948224865617243 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 570 0.1739878941054733 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 536 0.16360966884304157 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 536 0.16360966884304157 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 521 0.15903104005079224 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 495 0.1510947501442268 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 494 0.15078950822474352 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 485 0.14804233094939395 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 457 0.13949555720386192 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 451 0.1376641056869622 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 451 0.1376641056869622 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 435 0.13278023497522962 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 430 0.1312540253778132 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 413 0.1260649127465973 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 395 0.12057055819589815 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 389 0.11873910667899845 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 385 0.11751813900106528 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 373 0.11385523596726585 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 373 0.11385523596726585 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 373 0.11385523596726585 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 362 0.11049757485294971 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 362 0.11049757485294971 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 356 0.10866612333604998 No Hit CTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG 348 0.10622418798018368 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 343 0.10469797838276726 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 338 0.10317176878535082 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.10483838966573E-4 0.0 18-19 0.0 0.0 0.0 7.631047987082162E-4 0.0 20-21 0.0 0.0 0.0 0.001984072476641362 0.0 22-23 0.0 0.0 0.0 0.0025945563156079352 0.0 24-25 0.0 0.0 0.0 0.004426007832507654 0.0 26-27 0.0 0.0 0.0 0.007173185107857232 0.0 28-29 0.0 0.0 0.0 0.012514918698814746 0.0 30-31 0.0 0.0 0.0 0.0215195553235717 0.0 32-33 0.0 0.0 0.0 0.028997982350912214 0.0 34-35 0.0 0.0 0.0 0.03907096569386067 0.0 36-37 0.0 0.0 0.0 0.048533465197842546 0.0 38-39 0.0 0.0 0.0 0.05646975510440799 0.0 40-41 0.0 0.0 0.0 0.07005302052141424 0.0 42-43 0.0 0.0 0.0 0.09065685008653608 0.0 44-45 0.0 0.0 0.0 0.11446571980623244 0.0 46-47 0.0 0.0 0.0 0.17887176481720587 0.0 48-49 0.0 0.0 0.0 0.3131782093898519 0.0 50-51 0.0 0.0 0.0 0.39711973724775573 0.0 52-53 0.0 0.0 0.0 0.48304533758230084 0.0 54-55 0.0 0.0 0.0 0.5836225500520438 0.0 56-57 0.0 0.0 0.0 0.6588646832046738 0.0 58-59 0.0 0.0 0.0 0.7579156860770003 0.0 60-61 0.0 0.0 0.0 0.9557124499021699 0.0 62-63 0.0 0.0 0.0 1.0869664752799832 0.0 64-65 0.0 0.0 0.0 1.1962430824549997 0.0 66-67 0.0 0.0 0.0 1.3821354114203213 0.0 68-69 0.0 0.0 0.0 1.5912261262663723 0.0 70-71 0.0 0.0 0.0 1.773302931238153 0.0 72-73 0.0 0.0 0.0 1.9985714678168183 0.0 74-75 0.0 0.0 0.0 2.181411377587307 0.0 76-77 0.0 0.0 0.0 2.301066210024755 0.0 78-79 0.0 0.0 0.0 2.453381927846915 0.0 80-81 0.0 0.0 0.0 2.6981859472725107 0.0 82-83 0.0 0.0 0.0 2.9344431929525747 0.0 84-85 0.0 0.0 0.0 3.3065330928027006 0.0 86-87 0.0 0.0 0.0 3.9910380972439707 0.0 88-89 0.0 0.0 0.0 4.621057419057474 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACGC 35 3.4108434E-6 67.85954 3 GTCTAAG 30 1.2996147E-4 63.335575 1 CGTCACT 30 1.2996147E-4 63.335575 1 ACAACGC 25 0.005017375 57.002018 3 TACCTGG 305 0.0 56.067554 2 TATAACG 45 1.51325075E-5 52.779644 2 ACCTGGG 330 0.0 48.941124 3 GGACAAT 50 2.8193952E-5 47.508938 6 GTATAAC 50 2.8219418E-5 47.501682 1 GGTGAGT 70 4.2951833E-6 40.721943 8 CGCAGGG 60 8.26859E-5 39.59078 8 CTGGGGA 60 8.2760365E-5 39.584736 5 GTAGCCC 50 0.0016138478 38.001343 3 GTCACTA 50 0.0016138478 38.001343 2 GTGTAGC 80 1.0749976E-5 35.62626 1 ATGGGTA 235 0.0 34.362915 5 CCTGGGG 195 0.0 34.10377 4 GACAATG 70 2.0478739E-4 33.93495 7 AGTCCTG 70 2.0497123E-4 33.92977 5 GTACCAG 85 1.628261E-5 33.530598 1 >>END_MODULE