##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936634_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 339820 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.535760108292624 31.0 31.0 34.0 30.0 34.0 2 31.36015243364134 31.0 31.0 34.0 28.0 34.0 3 31.345347536931317 31.0 31.0 34.0 28.0 34.0 4 35.06930433759049 37.0 35.0 37.0 32.0 37.0 5 35.04618915896651 37.0 35.0 37.0 32.0 37.0 6 35.30012359484433 37.0 35.0 37.0 33.0 37.0 7 35.22501912777353 37.0 35.0 37.0 32.0 37.0 8 35.244938496851276 37.0 35.0 37.0 32.0 37.0 9 36.66845388735213 39.0 37.0 39.0 32.0 39.0 10-11 36.53073097522218 39.0 37.0 39.0 32.0 39.0 12-13 36.39903919722205 39.0 36.0 39.0 32.0 39.0 14-15 37.08402830910482 39.0 36.0 41.0 31.5 41.0 16-17 37.13889706315108 39.0 36.5 41.0 31.5 41.0 18-19 37.49384821376023 39.5 37.0 41.0 32.0 41.0 20-21 37.567624036254486 40.0 37.0 41.0 32.0 41.0 22-23 37.4125801895121 39.0 36.0 41.0 32.0 41.0 24-25 37.19149549761639 39.0 36.0 41.0 32.0 41.0 26-27 36.79738243776117 39.0 35.0 41.0 31.0 41.0 28-29 36.69876110882232 39.0 35.0 40.5 31.0 41.0 30-31 36.39256665293391 38.0 35.0 40.0 31.0 41.0 32-33 35.898974457065506 38.0 35.0 40.0 30.0 41.0 34-35 35.55804249308457 38.0 35.0 40.0 30.0 41.0 36-37 35.0744761932788 37.0 35.0 40.0 29.0 41.0 38-39 34.6458257312695 36.5 34.5 40.0 26.0 41.0 40-41 34.25101671473133 36.5 34.5 40.0 25.0 41.0 42-43 33.81792566652934 36.0 34.0 40.0 23.5 41.0 44-45 33.15301777411571 36.0 33.0 40.0 17.0 41.0 46-47 32.08611029368489 36.0 33.0 40.0 3.5 41.0 48-49 31.170680065917253 35.0 31.0 40.0 2.0 41.0 50-51 30.15411688540992 35.0 29.5 39.0 2.0 40.0 52-53 30.124084809605087 35.0 29.0 39.0 2.0 40.0 54-55 30.404169854628922 35.0 30.0 39.0 2.0 41.0 56-57 30.381347478076627 35.0 30.5 40.0 2.0 41.0 58-59 30.1847433935613 35.0 29.5 39.5 2.0 41.0 60-61 29.774168677535165 35.0 28.5 39.0 2.0 41.0 62-63 29.29026249190748 35.0 28.0 39.0 2.0 40.5 64-65 28.772230886940143 35.0 26.0 38.0 2.0 40.0 66-67 28.357624624801367 35.0 26.0 37.5 2.0 40.0 68-69 28.084706609381435 35.0 26.0 37.0 2.0 39.0 70-71 27.60133158731093 35.0 25.0 36.5 2.0 39.0 72-73 26.98847625213348 34.0 23.0 35.5 2.0 39.0 74-75 26.545856629980577 34.0 21.0 35.0 2.0 37.5 76-77 26.00025307515744 33.5 19.0 35.0 2.0 37.0 78-79 25.537428638691072 33.0 14.5 35.0 2.0 36.5 80-81 25.169754575951977 33.0 7.0 35.0 2.0 36.0 82-83 24.75655935495262 33.0 2.0 35.0 2.0 36.0 84-85 24.3253413571891 33.0 2.0 35.0 2.0 35.0 86-87 23.74224736625272 32.0 2.0 35.0 2.0 35.0 88-89 23.11006268024248 31.5 2.0 35.0 2.0 35.0 90-91 22.548613972102878 31.0 2.0 35.0 2.0 35.0 92-93 22.214910835148018 31.0 2.0 35.0 2.0 35.0 94-95 21.817137013713143 31.0 2.0 35.0 2.0 35.0 96-97 21.01850832793832 30.5 2.0 34.5 2.0 35.0 98-99 20.248131363663113 29.0 2.0 34.0 2.0 35.0 100-101 18.73768171384851 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10291303828618936 1101 2 0.3419072091010307 1101 3 0.32801988315986463 1101 4 0.523839346307625 1101 5 0.16573064072402133 1101 6 0.0029191502188652407 1101 7 0.08351363079427188 1101 8 -0.07950636374368969 1101 9 0.3072780581854957 1101 10-11 0.1702748732578172 1101 12-13 0.1508442673751702 1101 14-15 0.49564290632083186 1101 16-17 0.36106313237441157 1101 18-19 0.4949971521016323 1101 20-21 0.3485905202042545 1101 22-23 0.06334596895788991 1101 24-25 0.46724089228205656 1101 26-27 0.5874413184269898 1101 28-29 0.7647042938833408 1101 30-31 0.275902063585562 1101 32-33 0.46049501321139985 1101 34-35 0.5236867930437583 1101 36-37 0.11019236585762826 1101 38-39 8.718725837439933E-4 1101 40-41 0.1681402434249577 1101 42-43 -0.1442485084956857 1101 44-45 -0.1454027719642994 1101 46-47 -0.4587119503880288 1101 48-49 -0.7257466475910519 1101 50-51 -0.7225218765084129 1101 52-53 -0.6491422439959251 1101 54-55 -0.8439865142949756 1101 56-57 -0.7787337816506117 1101 58-59 -0.5835783856007204 1101 60-61 -0.8082309305769115 1101 62-63 -0.7075694129028243 1101 64-65 -0.30201270973333294 1101 66-67 -0.45491303753330214 1101 68-69 -0.5296606438609146 1101 70-71 -0.23022460802697253 1101 72-73 -0.0017443534031684749 1101 74-75 -0.30665996236481874 1101 76-77 -0.3526873981091718 1101 78-79 -0.5294392873839762 1101 80-81 -0.5056345041493309 1101 82-83 -0.5169400719469586 1101 84-85 -0.3937456415208622 1101 86-87 -0.9511689865799298 1101 88-89 -0.8305785261456045 1101 90-91 -0.27666872575713697 1101 92-93 -0.27564321622954324 1101 94-95 -0.6050984445278829 1101 96-97 -0.03213921767141059 1101 98-99 -0.3049502654937193 1101 100-101 -0.05355265759822103 1102 1 0.19343711238991546 1102 2 0.1361604875664355 1102 3 0.17417619270464257 1102 4 0.2732749218604411 1102 5 0.16093503442808554 1102 6 0.11580403965572117 1102 7 -0.08386023435231493 1102 8 0.2135380056220697 1102 9 -0.0409197865937756 1102 10-11 0.2800734417813473 1102 12-13 0.15746924936161832 1102 14-15 0.07880944755984842 1102 16-17 -0.14213527282629457 1102 18-19 0.08598157238568405 1102 20-21 0.15436846521890146 1102 22-23 -0.1257482298796404 1102 24-25 0.21331393921335007 1102 26-27 0.2045453806818074 1102 28-29 0.11131694659817981 1102 30-31 0.14632061833874133 1102 32-33 0.33942396778523687 1102 34-35 0.24956865620485758 1102 36-37 0.26195849398598625 1102 38-39 0.3892319193389113 1102 40-41 0.3341410760510968 1102 42-43 0.7518173330171365 1102 44-45 0.8312756902392806 1102 46-47 1.0146119892145578 1102 48-49 0.7439425586920727 1102 50-51 0.47509473118056533 1102 52-53 0.6302558633808211 1102 54-55 0.5307512885327554 1102 56-57 0.7161463682220948 1102 58-59 0.7301055046847758 1102 60-61 0.43094092665972994 1102 62-63 0.5913553785403387 1102 64-65 0.6653947806994722 1102 66-67 0.3385116178075158 1102 68-69 0.49207570187107663 1102 70-71 0.600111804955592 1102 72-73 0.6133181736171522 1102 74-75 0.8636521123153393 1102 76-77 0.906439465292106 1102 78-79 0.706241944902704 1102 80-81 0.6714146368046663 1102 82-83 0.6763172575648362 1102 84-85 0.9815165556514103 1102 86-87 0.7080707617107862 1102 88-89 0.6331261797932619 1102 90-91 0.03353654860918809 1102 92-93 0.05686282831595335 1102 94-95 -0.03686713458280266 1102 96-97 -0.22016861577878544 1102 98-99 0.2336827865614275 1102 100-101 0.3097565509265898 1103 1 0.2173212095946475 1103 2 0.2109133835624064 1103 3 0.30865900559811976 1103 4 0.3153691676423591 1103 5 0.3984224431412571 1103 6 0.05490942872648219 1103 7 0.1892841247511825 1103 8 -0.07065520767432787 1103 9 0.4337559877703612 1103 10-11 0.38491951329985596 1103 12-13 0.23310747021279354 1103 14-15 0.6030555442609327 1103 16-17 0.8762283320591138 1103 18-19 0.4817532608772481 1103 20-21 0.4089721544659497 1103 22-23 0.16503573511658942 1103 24-25 0.2119367672163719 1103 26-27 -0.04718985603438597 1103 28-29 -0.08486710653704677 1103 30-31 0.16791467525135317 1103 32-33 0.12389655025816637 1103 34-35 0.3319746937268917 1103 36-37 0.44489415251028674 1103 38-39 -0.08100542641783193 1103 40-41 -0.5202957786454547 1103 42-43 -0.34654422578417865 1103 44-45 -0.3248562664466874 1103 46-47 0.005628893017121328 1103 48-49 1.7131089554567325E-4 1103 50-51 -0.5090677908541856 1103 52-53 -0.38225279312887395 1103 54-55 0.006332061637763786 1103 56-57 0.031097734376711372 1103 58-59 -0.29126033515405325 1103 60-61 -0.06267293005142704 1103 62-63 -0.4286923109585281 1103 64-65 -0.09975804604442118 1103 66-67 0.0228441222144653 1103 68-69 -0.11875516566858835 1103 70-71 0.09165203411701128 1103 72-73 0.3357983423403965 1103 74-75 0.6561044228164725 1103 76-77 0.619806690172485 1103 78-79 0.4146867635881719 1103 80-81 0.576086791173843 1103 82-83 0.7992711734550362 1103 84-85 0.6295153280009487 1103 86-87 0.5047805010711492 1103 88-89 0.5434526089043175 1103 90-91 0.770756392477228 1103 92-93 0.4493961952260541 1103 94-95 0.2155055360291307 1103 96-97 0.46856690424136005 1103 98-99 0.33959124790617423 1103 100-101 0.3801457790807099 1104 1 0.23557980910324972 1104 2 0.1326117865108074 1104 3 0.11920875989787305 1104 4 0.3062014772246684 1104 5 0.3701362023550203 1104 6 0.2899524262694797 1104 7 0.5346618888789507 1104 8 0.5028245220553984 1104 9 0.6615508280652023 1104 10-11 0.4505982602285954 1104 12-13 0.6395647797951014 1104 14-15 0.33547262042800696 1104 16-17 0.3768809733367604 1104 18-19 0.706760939009925 1104 20-21 0.6270157662595537 1104 22-23 0.30977621423206614 1104 24-25 0.3524619514915557 1104 26-27 0.47646647074694215 1104 28-29 0.4655244782295398 1104 30-31 0.37172302905970156 1104 32-33 0.48500680824342624 1104 34-35 0.5184688583460613 1104 36-37 0.8325170026331321 1104 38-39 0.7595658266534286 1104 40-41 0.9334972188975428 1104 42-43 0.7089072484172974 1104 44-45 0.8027850111945867 1104 46-47 1.105590502353735 1104 48-49 0.9826114214606605 1104 50-51 0.5046223197109256 1104 52-53 1.192155990654907 1104 54-55 1.371550120605825 1104 56-57 1.193543987447967 1104 58-59 1.320702195558475 1104 60-61 1.4289195146660205 1104 62-63 1.355022369631154 1104 64-65 1.3487293618179876 1104 66-67 0.8334169109122556 1104 68-69 1.1056229055844824 1104 70-71 1.0027546139695893 1104 72-73 0.9232829860750371 1104 74-75 0.8556974817845351 1104 76-77 1.114263078378876 1104 78-79 1.3471268741532825 1104 80-81 1.3972400467020982 1104 82-83 1.1525881267719917 1104 84-85 1.03936637222699 1104 86-87 0.9866063610220088 1104 88-89 0.76032914453085 1104 90-91 0.33218145390229026 1104 92-93 -0.046304050474194725 1104 94-95 -0.1215568104082152 1104 96-97 0.3421694840939402 1104 98-99 0.13022392603885535 1104 100-101 -0.0491002282902997 1105 1 0.0927858086684985 1105 2 0.005461580004034516 1105 3 -0.11210781136507819 1105 4 0.263204592940923 1105 5 0.2731939517934663 1105 6 0.09769824179009134 1105 7 0.19622264534586975 1105 8 -0.049961507942427374 1105 9 0.18218701384883929 1105 10-11 0.3183904492004146 1105 12-13 0.32860542902341905 1105 14-15 0.2587571650262319 1105 16-17 0.34503545489606324 1105 18-19 0.375752316446345 1105 20-21 -0.01630355842706166 1105 22-23 -0.022909823011609376 1105 24-25 -0.0231122822596177 1105 26-27 -0.33616667177896176 1105 28-29 0.23529116180318965 1105 30-31 -0.12169049169965973 1105 32-33 -0.35095797691706565 1105 34-35 -0.7147098968166077 1105 36-37 -0.6782322184377989 1105 38-39 -0.6598349414068636 1105 40-41 -0.6528588422574444 1105 42-43 -0.14809949022482982 1105 44-45 0.09386174476460951 1105 46-47 0.09025828005099612 1105 48-49 0.45415696163890473 1105 50-51 0.4386343478248307 1105 52-53 0.6719110808657653 1105 54-55 0.2603154274676456 1105 56-57 0.6402290102724031 1105 58-59 0.7770966597277393 1105 60-61 0.8451574684374847 1105 62-63 0.7340963497775199 1105 64-65 1.2345057548329876 1105 66-67 0.32596455238751076 1105 68-69 0.257535810446452 1105 70-71 0.6004794165201339 1105 72-73 0.7118417861619903 1105 74-75 0.6109507243031906 1105 76-77 1.0526226175427063 1105 78-79 0.849235417012494 1105 80-81 0.7046779135233443 1105 82-83 0.8419838227360508 1105 84-85 0.8632124703358244 1105 86-87 1.2377693202278621 1105 88-89 1.4582024870422643 1105 90-91 1.5648095243319702 1105 92-93 0.7041488152601723 1105 94-95 0.7282439286686646 1105 96-97 1.0713956271270533 1105 98-99 0.5816549817594527 1105 100-101 0.9329844032581391 1106 1 -0.3364014158191537 1106 2 0.004275046409361494 1106 3 -0.044512945000303006 1106 4 0.2683787805784448 1106 5 -0.10318501802503022 1106 6 -0.07303493083552581 1106 7 0.029612301476419134 1106 8 -0.2885298575342716 1106 9 -0.3539158991662319 1106 10-11 0.007158181494403948 1106 12-13 0.15482331564187035 1106 14-15 0.13471934614532444 1106 16-17 0.05187529538990532 1106 18-19 -0.1881402647148036 1106 20-21 0.11997132686217782 1106 22-23 -0.11021015869548023 1106 24-25 -0.2893125961653453 1106 26-27 -0.08893212112224091 1106 28-29 -0.42485707979908227 1106 30-31 -0.0810667354947654 1106 32-33 -0.1213487069356205 1106 34-35 0.11341170034771864 1106 36-37 0.05205292511023174 1106 38-39 0.08745968690022465 1106 40-41 0.2875966965264354 1106 42-43 -0.06268409082110082 1106 44-45 -0.19294895806879708 1106 46-47 0.46679119884854714 1106 48-49 -0.05242529592900169 1106 50-51 -0.008005434093298902 1106 52-53 0.24086520407000833 1106 54-55 0.39150999644805395 1106 56-57 -0.0221195752744201 1106 58-59 0.33045312295645957 1106 60-61 0.3478384335849398 1106 62-63 0.5621841532635266 1106 64-65 0.42143736393775555 1106 66-67 0.4390532194897432 1106 68-69 0.53127586947274 1106 70-71 0.47393335573656614 1106 72-73 0.24193693414075312 1106 74-75 0.0011475537051914841 1106 76-77 0.15338034690790892 1106 78-79 0.2843590643266509 1106 80-81 0.14302322483821683 1106 82-83 0.4851000504603853 1106 84-85 0.4372844583803719 1106 86-87 0.46383400883788894 1106 88-89 0.36678307745537353 1106 90-91 0.7009830285862186 1106 92-93 0.7319910513503203 1106 94-95 0.5131922243408198 1106 96-97 0.45466475247155813 1106 98-99 0.7105328063477323 1106 100-101 0.6842066079577123 1107 1 0.6069176045789781 1107 2 0.3779392456313708 1107 3 0.4696111999554873 1107 4 0.4900360955420524 1107 5 0.4883519102258944 1107 6 0.34977619988635666 1107 7 0.5630106137793973 1107 8 0.5285806857425968 1107 9 0.4880271476449707 1107 10-11 0.9735754694127081 1107 12-13 0.7486345078303458 1107 14-15 1.1644779580540359 1107 16-17 0.9660108555700901 1107 18-19 0.8977505179305396 1107 20-21 0.8771343801712774 1107 22-23 0.8162406724206619 1107 24-25 1.0970268926082198 1107 26-27 1.143885770924868 1107 28-29 1.0122625956573046 1107 30-31 0.9320212583234522 1107 32-33 0.7745137132675737 1107 34-35 0.6707775934134119 1107 36-37 0.5367828672438293 1107 38-39 0.7279679278141487 1107 40-41 0.7268633122636317 1107 42-43 0.7787496613631575 1107 44-45 1.0027299404498606 1107 46-47 1.1768181563400049 1107 48-49 1.154804931585204 1107 50-51 0.7536183376724601 1107 52-53 1.0099396049902474 1107 54-55 1.0669707763713063 1107 56-57 0.6138269663516347 1107 58-59 0.5636573764446915 1107 60-61 0.7710359257479489 1107 62-63 0.7242759492295612 1107 64-65 1.1474500260558926 1107 66-67 0.6147818181392317 1107 68-69 0.4871911509458329 1107 70-71 0.8611465482235943 1107 72-73 0.5447521040958812 1107 74-75 0.7724347519700778 1107 76-77 0.474332552241453 1107 78-79 0.5701040833373909 1107 80-81 0.5039374965029957 1107 82-83 0.6245062831729058 1107 84-85 0.8026638389727339 1107 86-87 0.9203859085946569 1107 88-89 1.1389659317883272 1107 90-91 1.3945808438878906 1107 92-93 1.4830952547871838 1107 94-95 1.7245670564053093 1107 96-97 1.9377087537711404 1107 98-99 1.8056962635801561 1107 100-101 2.2286469546292977 1108 1 0.42889417610182434 1108 2 0.27097119855443097 1108 3 0.30914079719833154 1108 4 0.1970976365418835 1108 5 0.3257549790264278 1108 6 0.3805008694494987 1108 7 0.3354995686639981 1108 8 0.26139888589292326 1108 9 0.5548019739479244 1108 10-11 0.3870294282387121 1108 12-13 0.491572050223418 1108 14-15 0.7240849169350341 1108 16-17 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70-71 -0.7340498375407414 2316 72-73 -0.5835532619376806 2316 74-75 -0.7092866199642778 2316 76-77 -1.6624459836243055 2316 78-79 -1.7848286107433786 2316 80-81 -1.6034753746015582 2316 82-83 -1.7296058318925525 2316 84-85 -1.400180527614026 2316 86-87 -1.901151985559867 2316 88-89 -2.0707519365502307 2316 90-91 -1.9816500246350692 2316 92-93 -2.2836251951482787 2316 94-95 -2.295841389104556 2316 96-97 -2.3975761438869796 2316 98-99 -2.8928285381900842 2316 100-101 -3.130499274395227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 631.0 3 215.0 4 177.0 5 218.0 6 249.0 7 334.0 8 442.0 9 649.0 10 1041.0 11 1811.0 12 3049.0 13 4288.0 14 4634.0 15 6218.0 16 8486.0 17 11712.0 18 9162.0 19 4368.0 20 3858.0 21 3714.0 22 4042.0 23 4497.0 24 4859.0 25 5377.0 26 6198.0 27 6861.0 28 7747.0 29 9300.0 30 10505.0 31 12555.0 32 15522.0 33 18744.0 34 24927.0 35 37401.0 36 38561.0 37 38323.0 38 24998.0 39 4133.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 58.18872655780935 15.740555431289218 12.64965918321698 13.421058827684448 2 14.304969423010142 23.939222714671658 42.01349036780675 19.742317494511443 3 23.552748526996723 36.88806352308245 28.3446445073606 11.21454344256023 4 10.378752642083757 17.158089339228834 38.14462427949861 34.3185337391888 5 9.775529672245057 43.99547761774093 30.13743802334209 16.091554686671927 6 21.05554508621502 36.308065579949115 22.472145010835767 20.164244323000094 7 23.473157177463744 41.817346947786085 19.281385306869726 15.428110567880449 8 25.725756367200493 27.673370635380824 21.090569803001628 25.510303194417055 9 31.6444407818253 19.080316332567126 21.325371060926688 27.949871824680887 10-11 23.338490322181773 27.940771664137237 24.392017511191057 24.32872050248993 12-13 33.221166796938824 26.784691770185194 24.9212847042822 15.07285672859378 14-15 28.042865299827525 25.212796016034755 32.546164668208924 14.198174015928796 16-17 16.68430262420105 30.14262314141781 33.609965223559676 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15.31486064558277 31.124546447058272 38.03238213509036 15.528210772268602 44-45 15.854726195398358 32.085393899889205 34.913857159883285 17.146022744829146 46-47 15.90118537527074 30.432626659760807 32.182291176193615 21.483896788774835 48-49 18.03082826689581 30.452330365077778 32.2543243736353 19.262516994391117 50-51 20.702359984873965 31.571474184952542 31.90195152312385 15.824214307049644 52-53 16.807405216669682 31.966411086048417 33.859649897223335 17.366533800058562 54-55 14.717380908589295 31.735589479492365 34.97016737171234 18.576862240205998 56-57 17.449795769425446 34.435658540604805 30.032782832860523 18.081762857109226 58-59 16.2949850944801 31.847195613980876 31.519068176935733 20.33875111460329 60-61 16.846347503277514 29.763504019092714 33.15387320472433 20.236275272905445 62-63 21.725689498899392 29.52191211611126 31.01788636069356 17.734512024295785 64-65 19.868071976257077 30.75250179146073 33.52746342403691 15.851962808245283 66-67 16.944247031074582 31.450671916636136 34.1682079623556 17.436873089933684 68-69 15.967786317277993 34.96505966123543 32.439113755854656 16.62804026563192 70-71 16.16035717333914 34.19075340147567 29.554798854549574 20.09409057063562 72-73 16.16971059617071 32.51025597259581 28.873726464235055 22.446306966998428 74-75 16.827198968826423 32.83143078276787 31.04835980424182 19.293010444163894 76-77 18.688265042619467 30.313896462576828 32.31045629977973 18.68738219502398 78-79 15.997515429214019 30.634110154211186 34.825204263217316 18.543170153357483 80-81 15.135972826038966 35.11922457665783 32.3294195215711 17.41538307573211 82-83 14.720899572108792 34.20113241084619 30.107325944803797 20.97064207224122 84-85 15.971829825304892 31.164506521636763 30.273961717756748 22.589701935301594 86-87 17.98327513622244 30.951739806676702 31.632888798638 19.43209625846286 88-89 19.58057495839446 27.980258888723757 32.6759829722615 19.76318318062028 90-91 16.244410010550897 29.55605293401789 34.75365786542234 19.445879190008874 92-93 14.770912890242736 32.74543996350499 32.88509223083093 19.59855491542135 94-95 14.373937802105635 33.69780975713834 31.13926471994762 20.78898772080841 96-97 14.524421215572369 31.300561789486387 29.20466617815407 24.970350816787175 98-99 16.318309991817223 30.621096256527085 29.979572494098395 23.081021257557293 100-101 18.647293645232892 30.72550208724631 30.993399709139723 19.633804558381073 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1394.0 1 1051.5 2 2345.0 3 3288.5 4 2052.0 5 1663.0 6 2428.5 7 4176.5 8 5661.5 9 5617.5 10 4245.5 11 3066.5 12 2931.0 13 2999.0 14 2938.5 15 2749.5 16 2487.5 17 2306.5 18 2288.0 19 2354.5 20 2366.0 21 2462.0 22 2648.0 23 2794.5 24 2983.0 25 3285.5 26 3651.0 27 3971.5 28 4246.5 29 4627.5 30 5084.0 31 5356.0 32 5571.5 33 5933.5 34 6360.0 35 6543.5 36 6809.0 37 7224.5 38 7105.0 39 6601.0 40 6195.0 41 5965.0 42 5849.5 43 5964.0 44 7740.0 45 8453.5 46 8785.5 47 14077.0 48 34972.5 49 33616.5 50 13883.0 51 9443.5 52 5125.5 53 3353.0 54 2715.0 55 2333.0 56 2174.0 57 2114.5 58 2037.5 59 2112.0 60 2265.0 61 2270.0 62 1917.0 63 1059.5 64 465.0 65 322.5 66 247.0 67 205.0 68 149.0 69 122.5 70 132.5 71 112.0 72 93.5 73 143.0 74 141.0 75 78.5 76 53.0 77 39.0 78 36.0 79 30.0 80 21.5 81 14.5 82 6.0 83 3.5 84 5.0 85 3.0 86 0.0 87 0.5 88 2.0 89 1.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014125125066211523 2 0.006474015655346948 3 0.011182390677417456 4 0.03707845329880525 5 0.051792125242775586 6 0.06003178153139898 7 0.024424695426990762 8 0.020893414160437877 9 0.015007945382849745 10-11 0.04458242599023012 12-13 0.04311105879583309 14-15 0.017067859455005593 16-17 0.02310046495203343 18-19 0.03237007827673474 20-21 0.025601789182508387 22-23 0.013683714907892412 24-25 0.0019127773527161437 26-27 0.0022070507915955507 28-29 0.0010299570360779236 30-31 0.005591195338708728 32-33 0.001618503913836737 34-35 0.0011770937555176271 36-37 0.006915425813666059 38-39 0.005738332058148432 40-41 0.0036784179859925843 42-43 8.828203166382202E-4 44-45 0.0019127773527161437 46-47 0.0035312812665528807 48-49 5.885468777588136E-4 50-51 0.0025013242304749573 52-53 0.001618503913836737 54-55 0.0022070507915955507 56-57 0.0023541875110352543 58-59 0.0038255547054322873 60-61 0.0013242304749573304 62-63 0.0023541875110352543 64-65 0.0039726914248719904 66-67 0.0025013242304749573 68-69 0.007798246130304279 70-71 0.012065210994055676 72-73 0.005296921899829322 74-75 0.0038255547054322873 76-77 0.0030898711082337706 78-79 0.036342769701606735 80-81 0.024424695426990762 82-83 0.004119828144311694 84-85 0.008239656288623389 86-87 0.007798246130304279 88-89 0.006326878935907245 90-91 0.01132952739685716 92-93 0.01397798834677182 94-95 0.005149785180389618 96-97 0.0038255547054322873 98-99 0.024130421988111352 100-101 0.04060973456535813 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 339820.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.23758542864306 #Duplication Level Percentage of deduplicated Percentage of total 1 85.27056057666918 36.86893147482017 2 8.556252409961909 7.399033890495222 3 2.4991894307593037 3.2417674954445164 4 1.1213019324938416 1.939295523900201 5 0.5478447497344283 1.184374208413796 6 0.389155333626673 1.0095682189617217 7 0.23222171271769693 0.7028494299412057 8 0.16520738425761006 0.571453471222486 9 0.14401955986885592 0.5604352220902709 >10 0.8674520108939295 7.130647085273546 >50 0.0887238840665072 2.6947875317083603 >100 0.08735890123471479 7.913208765910139 >500 0.012967336902027976 3.970768837786651 >1k 0.013649828317924183 12.086116177855816 >5k 0.004094948495377255 12.726762666175883 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9161 2.6958389735742454 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8919 2.6246248013654285 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7379 2.1714437054911424 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6924 2.0375492908010124 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5714 1.6814784297569303 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5031 1.4804896710022952 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4788 1.4089812253545995 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3648 1.0735095050320758 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3295 0.9696309811076452 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3199 0.9413807309752222 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3071 0.9037137307986581 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2329 0.6853628391501383 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2126 0.6256253310576187 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1907 0.5611794479430287 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1719 0.5058560414337002 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1558 0.4584780177741157 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1475 0.434053322347125 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1460 0.42963922076393385 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1397 0.41109999411453124 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1360 0.40021187687599313 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1310 0.3854982049320228 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1300 0.3825554705432288 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1299 0.38226119710434936 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1292 0.3802012830321935 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1274 0.3749043611323642 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1150 0.33841445471131776 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 994 0.2925077982461303 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 953 0.28044258725207466 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 938 0.27602848566888355 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 806 0.23718439173680186 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 793 0.23335883703136956 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 783 0.23041610264257548 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 754 0.2218821729150727 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 743 0.2186451650873992 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 731 0.21511388382084634 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 715 0.21040550879877581 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 708 0.20834559472661995 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 699 0.20569713377670534 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 593 0.1745041492554882 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 562 0.1653816726502266 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 561 0.16508739921134719 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 551 0.1621446648225531 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 548 0.1612618445059149 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 517 0.1521393679006533 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 507 0.14919663351185922 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 485 0.14272261785651227 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 476 0.1400741569065976 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 473 0.13919133658995939 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 465 0.13683714907892414 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 456 0.13418868812900947 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 441 0.12977458654581836 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 437 0.12859749279030075 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 422 0.12418339120710965 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 417 0.1227120240127126 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 386 0.11358954740745099 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 380 0.11182390677417456 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 377 0.11094108645753635 No Hit CTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG 375 0.11035253957977753 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 372 0.1094697192631393 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 369 0.10858689894650109 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 364 0.10711553175210406 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 362 0.10652698487434524 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 360 0.10593843799658643 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 344 0.10123006297451592 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.414101583191101E-4 0.0 18-19 0.0 0.0 0.0 5.885468777588135E-4 0.0 20-21 0.0 0.0 0.0 0.0010299570360779236 0.0 22-23 0.0 0.0 0.0 0.0019127773527161437 0.0 24-25 0.0 0.0 0.0 0.0044141015831911014 0.0 26-27 0.0 0.0 0.0 0.007798246130304278 0.0 28-29 0.0 0.0 0.0 0.011035253957977754 0.0 30-31 0.0 0.0 0.0 0.01868636336884233 0.0 32-33 0.0 0.0 0.0 0.024571832146430463 0.0 34-35 0.0 0.0 0.0 0.03163439467953622 0.0 36-37 0.0 0.0 0.0 0.042522511918074275 0.0 38-39 0.0 0.0 0.0 0.05208639868165499 0.0 40-41 0.0 0.0 0.0 0.06650579718674593 0.0 42-43 0.0 0.0 0.0 0.08681066446942498 0.0 44-45 0.0 0.0 0.0 0.11373668412689071 0.0 46-47 0.0 0.0 0.0 0.1746512859749279 0.0 48-49 0.0 0.0 0.0 0.2935377552822082 0.0 50-51 0.0 0.0 0.0 0.37931846271555525 1.4713671943970336E-4 52-53 0.0 0.0 0.0 0.4487669942910953 2.9427343887940673E-4 54-55 0.0 0.0 0.0 0.5438173150491437 2.9427343887940673E-4 56-57 0.0 0.0 0.0 0.6017891825083868 2.9427343887940673E-4 58-59 0.0 0.0 0.0 0.6997822376552293 2.9427343887940673E-4 60-61 0.0 0.0 0.0 0.8731092931551998 2.9427343887940673E-4 62-63 0.0 0.0 0.0 0.9853746100876934 2.9427343887940673E-4 64-65 0.0 0.0 0.0 1.0824848449178976 2.9427343887940673E-4 66-67 0.0 0.0 0.0 1.2616973691954563 2.9427343887940673E-4 68-69 0.0 0.0 0.0 1.4547407451003473 2.9427343887940673E-4 70-71 0.0 0.0 0.0 1.628362074039197 2.9427343887940673E-4 72-73 0.0 0.0 0.0 1.831410746865988 2.9427343887940673E-4 74-75 0.0 0.0 0.0 1.9969395562356542 2.9427343887940673E-4 76-77 0.0 0.0 0.0 2.0983167559296096 2.9427343887940673E-4 78-79 0.0 0.0 0.0 2.2433935612971574 2.9427343887940673E-4 80-81 0.0 0.0 0.0 2.4698369725148606 2.9427343887940673E-4 82-83 0.0 0.0 0.0 2.6920134188688127 2.9427343887940673E-4 84-85 0.0 0.0 0.0 3.0567653463598377 2.9427343887940673E-4 86-87 0.0 0.0 0.0 3.7085810134777235 2.9427343887940673E-4 88-89 0.0 0.0 0.0 4.290065328703431 2.9427343887940673E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 30 1.295769E-4 63.37419 1 GCGCCGG 30 1.296714E-4 63.364857 6 CGCCGGT 30 1.2986056E-4 63.346195 7 GCCGGTC 35 2.7823477E-4 54.29674 8 TCTTGCG 45 1.50923515E-5 52.804043 2 TACCTGG 315 0.0 52.804043 2 ACCTGGG 375 0.0 44.3554 3 ACGCCGA 65 2.5803529E-6 43.855057 7 CTTGCGC 55 4.941359E-5 43.203312 3 TAGGACG 60 8.2541665E-5 39.603035 4 GCCGAGT 60 8.268509E-5 39.591373 9 GTATAGG 50 0.0016091055 38.024517 1 CGTGGGC 25 0.0016172454 37.990936 48-49 CCTGGGG 195 0.0 36.55665 4 AACGCCG 80 1.0717136E-5 35.64273 6 CGTGGAA 55 0.002574512 34.55247 9 GACGTGG 55 0.002574512 34.55247 7 CCGGTCC 55 0.002574512 34.55247 9 CGCAAAG 55 0.002574512 34.55247 8 TAGTCAA 55 0.002574512 34.55247 9 >>END_MODULE