##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936634_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 339820 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.29462950974045 31.0 31.0 34.0 30.0 34.0 2 31.312456594667765 31.0 31.0 34.0 30.0 34.0 3 31.546701194750163 31.0 31.0 34.0 30.0 34.0 4 35.41910128891766 37.0 35.0 37.0 33.0 37.0 5 35.27356541698546 37.0 35.0 37.0 33.0 37.0 6 35.515793655464655 37.0 35.0 37.0 33.0 37.0 7 35.468306750632685 37.0 35.0 37.0 33.0 37.0 8 35.53261138249662 37.0 35.0 37.0 33.0 37.0 9 37.01359543287623 39.0 37.0 39.0 33.0 39.0 10-11 36.89345830145371 39.0 37.0 39.0 32.5 39.0 12-13 36.6987905361662 39.0 36.0 39.0 32.0 39.0 14-15 37.35714348772879 39.0 36.0 41.0 32.0 41.0 16-17 37.38711376611147 39.0 36.0 40.0 32.0 41.0 18-19 37.80776293331764 39.5 37.0 41.0 32.5 41.0 20-21 37.96398534518274 40.0 37.0 41.0 33.0 41.0 22-23 37.80719351421341 40.0 36.5 41.0 33.0 41.0 24-25 37.5972441292449 39.0 36.0 41.0 33.0 41.0 26-27 37.08784797834147 39.0 35.0 41.0 31.5 41.0 28-29 36.79279177211465 38.5 35.0 40.5 31.0 41.0 30-31 36.40432581955153 38.0 35.0 40.0 31.0 41.0 32-33 35.814953504796655 37.5 35.0 40.0 30.0 41.0 34-35 35.39850803366488 37.0 35.0 40.0 30.0 41.0 36-37 34.83010564416456 37.0 34.0 40.0 27.0 41.0 38-39 34.41002736742982 36.5 33.5 40.0 25.0 41.0 40-41 33.90371961626744 36.0 33.0 40.0 23.5 41.0 42-43 33.43497145547643 36.0 33.0 40.0 20.5 41.0 44-45 32.686894532399506 36.0 33.0 40.0 10.0 41.0 46-47 31.52238685186275 35.0 31.5 40.0 2.0 41.0 48-49 30.50425666529339 35.0 30.0 40.0 2.0 41.0 50-51 30.07972456006121 35.0 28.5 40.0 2.0 41.0 52-53 29.54476634688953 35.0 27.0 39.0 2.0 41.0 54-55 29.31066446942499 35.0 27.0 39.0 2.0 40.0 56-57 29.165650932846802 35.0 27.0 39.0 2.0 40.0 58-59 28.904618621623214 35.0 26.0 39.0 2.0 40.0 60-61 28.46906009063622 35.0 25.5 38.5 2.0 40.0 62-63 27.972360367253252 34.5 24.0 38.0 2.0 40.0 64-65 27.34754723088694 34.0 22.0 37.0 2.0 40.0 66-67 26.95986551703843 34.0 22.0 36.5 2.0 39.0 68-69 26.61660143605438 33.0 20.5 36.0 2.0 39.0 70-71 26.098911188276148 33.0 19.0 35.5 2.0 38.5 72-73 25.60788358542758 33.0 16.0 35.0 2.0 37.5 74-75 25.26952798540404 33.0 10.5 35.0 2.0 37.0 76-77 23.782259725737156 30.5 7.0 34.0 2.0 35.5 78-79 24.3920693308222 32.0 2.0 35.0 2.0 36.0 80-81 24.279593608380907 32.0 2.0 35.0 2.0 36.0 82-83 23.950089753398856 32.0 2.0 35.0 2.0 35.0 84-85 23.516317462185864 32.0 2.0 35.0 2.0 35.0 86-87 22.913309987640517 31.0 2.0 34.5 2.0 35.0 88-89 22.228357659937615 30.5 2.0 34.0 2.0 35.0 90-91 21.55730386675299 29.5 2.0 34.0 2.0 35.0 92-93 21.1850052969219 29.0 2.0 34.0 2.0 35.0 94-95 20.72743364133953 29.0 2.0 34.0 2.0 35.0 96-97 19.8875787181449 29.0 2.0 34.0 2.0 35.0 98-99 18.99812547819434 27.0 2.0 34.0 2.0 35.0 100-101 16.852483667824142 21.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.12821530776736978 1101 2 0.15993562332106848 1101 3 0.11661558511102754 1101 4 0.21073412902587307 1101 5 0.16528819099956138 1101 6 0.11236556194108971 1101 7 0.3314650899806537 1101 8 0.08113730416000209 1101 9 0.11512732341031295 1101 10-11 0.08786895658261074 1101 12-13 0.15378454655120777 1101 14-15 0.02766905908084283 1101 16-17 -0.18656792343690398 1101 18-19 -0.09480798534416834 1101 20-21 -0.28513863277117224 1101 22-23 -0.36172088921266976 1101 24-25 -0.07672744414452382 1101 26-27 0.05039346088978647 1101 28-29 0.26527961261370336 1101 30-31 0.3434717866801904 1101 32-33 0.038353152800112866 1101 34-35 0.39569432373812674 1101 36-37 -0.09186473092572811 1101 38-39 -0.30530893462285746 1101 40-41 0.10355131100751436 1101 42-43 0.12352209704909711 1101 44-45 0.20342282315438354 1101 46-47 0.2566141233680881 1101 48-49 0.14057629814802652 1101 50-51 -0.22270591143837137 1101 52-53 -0.37004504758213486 1101 54-55 -0.32744975110274055 1101 56-57 -0.18703099573576765 1101 58-59 -0.07144114044461602 1101 60-61 0.13242559270655008 1101 62-63 -0.7111973518999655 1101 64-65 -0.7246894770820198 1101 66-67 -0.8685070068470644 1101 68-69 -1.1885785967236124 1101 70-71 -1.163698713482031 1101 72-73 -0.9504520104178766 1101 74-75 -1.0464384389108865 1101 76-77 -0.39075210215534995 1101 78-79 -0.29286442419667225 1101 80-81 -0.8443134925268723 1101 82-83 -0.6842430480754942 1101 84-85 -0.5148507318774804 1101 86-87 -0.8134597324704451 1101 88-89 -0.8211467848522602 1101 90-91 -0.9404593802503562 1101 92-93 -0.9050073454382463 1101 94-95 -0.9493031232924487 1101 96-97 -0.9391541668821617 1101 98-99 -0.81677634754314 1101 100-101 -0.6140309888989783 1102 1 0.36938066411534365 1102 2 0.26525482335949846 1102 3 0.09525552234382673 1102 4 0.04026993995569228 1102 5 -0.2064553601509047 1102 6 -0.13795067586974596 1102 7 -0.21667511145082585 1102 8 0.0307474109603163 1102 9 0.020480148734954184 1102 10-11 -0.14728788135951731 1102 12-13 -0.37929633026816134 1102 14-15 0.04848871546244027 1102 16-17 0.18301800518141675 1102 18-19 0.02384924489297191 1102 20-21 -0.21219177673543754 1102 22-23 -0.24767512679932935 1102 24-25 -0.16669509982159525 1102 26-27 -0.5593690805413729 1102 28-29 -0.6196378453146494 1102 30-31 -0.3878061343163992 1102 32-33 -0.2541548131903113 1102 34-35 -0.17154423966155008 1102 36-37 -0.5686592804269566 1102 38-39 -0.3095160984100218 1102 40-41 -0.5192410288320346 1102 42-43 -0.5564132143050387 1102 44-45 -0.23800112765662362 1102 46-47 -0.3396542704640737 1102 48-49 -0.027610178082099424 1102 50-51 -0.07555925715434952 1102 52-53 0.12117234789926457 1102 54-55 0.4457516963857735 1102 56-57 0.2367156617506616 1102 58-59 0.12538287121214964 1102 60-61 0.6909576087826359 1102 62-63 0.33844397838040763 1102 64-65 0.0591879987798265 1102 66-67 0.2409112757014107 1102 68-69 0.3933630233747003 1102 70-71 -0.09169656786852798 1102 72-73 0.3516151440823059 1102 74-75 0.011509137666699587 1102 76-77 0.025460890014759485 1102 78-79 0.3894206541822669 1102 80-81 0.207573526511446 1102 82-83 0.4406068390396136 1102 84-85 1.0866616054001526 1102 86-87 0.9622756205150047 1102 88-89 0.6765995203692654 1102 90-91 1.1239730729544526 1102 92-93 1.1728792252629212 1102 94-95 0.849465550750427 1102 96-97 0.9545418925545306 1102 98-99 0.502996121353469 1102 100-101 0.3833950755014506 1103 1 0.388511550541935 1103 2 0.5412086133368348 1103 3 0.1794440139076663 1103 4 0.16580506964594832 1103 5 0.13267521149352035 1103 6 0.12729265074823815 1103 7 0.06228484825705749 1103 8 0.2780796320633172 1103 9 0.12970956198098804 1103 10-11 0.06384439010862764 1103 12-13 0.15527068685598522 1103 14-15 0.18194848630101745 1103 16-17 -0.03324915044688481 1103 18-19 0.29506162842356787 1103 20-21 -0.13333884296560683 1103 22-23 -0.005277116222650591 1103 24-25 -0.04971417562113345 1103 26-27 -0.13383867258368554 1103 28-29 -0.07046493546826582 1103 30-31 -0.02498426954682742 1103 32-33 0.08284704402449705 1103 34-35 0.04680441307076677 1103 36-37 -0.12751567680066245 1103 38-39 0.01029471750536004 1103 40-41 -0.13148054658575603 1103 42-43 -0.1150650022677695 1103 44-45 0.1959182251512246 1103 46-47 0.21087220271648377 1103 48-49 0.5232861641490771 1103 50-51 0.26294175014859533 1103 52-53 0.5462384521551193 1103 54-55 0.19570447906016142 1103 56-57 0.33793780352855407 1103 58-59 0.5695287097918502 1103 60-61 0.28598247657412657 1103 62-63 -0.10049221527358654 1103 64-65 0.11420798744767069 1103 66-67 0.36728844769838886 1103 68-69 0.5822519997030824 1103 70-71 0.42294080307078374 1103 72-73 0.6407937200077676 1103 74-75 0.7168218737533287 1103 76-77 0.5157852730417112 1103 78-79 0.5776525227292311 1103 80-81 0.6303810686922553 1103 82-83 0.8945305987978607 1103 84-85 0.994690124664821 1103 86-87 0.7615862475610875 1103 88-89 1.1071900517215738 1103 90-91 0.88369984203025 1103 92-93 0.37580748640620953 1103 94-95 0.3232599350365035 1103 96-97 0.11297569386402628 1103 98-99 0.29281213117467075 1103 100-101 0.4661631125198369 1104 1 0.38901830729869147 1104 2 0.427050136678357 1104 3 0.212459984423635 1104 4 0.06138246522333901 1104 5 -0.034493093174788214 1104 6 0.26376378096937003 1104 7 0.14496298093519044 1104 8 0.14950162223530583 1104 9 0.12197000424143312 1104 10-11 0.16671601173024442 1104 12-13 0.1418723453326436 1104 14-15 0.033683744286285844 1104 16-17 -0.11123594405868431 1104 18-19 0.03880395029089101 1104 20-21 0.019548134921372196 1104 22-23 0.2136110901655499 1104 24-25 0.08823115607419396 1104 26-27 0.2466128016177933 1104 28-29 0.21601387288554008 1104 30-31 0.27377955109199803 1104 32-33 -0.1720592827568339 1104 34-35 -0.33602728226093603 1104 36-37 -0.4572607627957481 1104 38-39 -0.4772820638214199 1104 40-41 -0.14084019032039663 1104 42-43 -0.22358772954049755 1104 44-45 -0.3859460254630207 1104 46-47 -0.18907405035476899 1104 48-49 0.07261816660608034 1104 50-51 -0.15434940464255575 1104 52-53 -0.171052702636036 1104 54-55 0.04714796800364951 1104 56-57 0.2755529755187247 1104 58-59 0.1285198031579462 1104 60-61 -0.17512010327845218 1104 62-63 -0.1399194265757977 1104 64-65 -0.12018236881768729 1104 66-67 -0.22796646630652262 1104 68-69 0.04069026751634652 1104 70-71 0.3322159873459647 1104 72-73 0.11839660698565169 1104 74-75 -0.3013829788265241 1104 76-77 0.20358472021615626 1104 78-79 0.16845411978082936 1104 80-81 0.6796593242205091 1104 82-83 0.3668739648912265 1104 84-85 0.41334798707268305 1104 86-87 0.20860713208196913 1104 88-89 0.04938906892058981 1104 90-91 -0.15284806951765972 1104 92-93 0.4487420687157915 1104 94-95 0.6906125148890823 1104 96-97 0.42949135725469034 1104 98-99 0.5475495390370781 1104 100-101 0.5614103483920729 1105 1 0.356926429898504 1105 2 0.5873790983478031 1105 3 0.18771540069781878 1105 4 -0.01491635370282296 1105 5 0.046899559531539126 1105 6 0.08862429832318242 1105 7 0.1454556988541782 1105 8 0.060913838741356585 1105 9 0.1368384920321759 1105 10-11 0.12056355900942606 1105 12-13 0.18355410752378987 1105 14-15 0.4690326320480338 1105 16-17 0.24066246909978162 1105 18-19 0.032010964275379195 1105 20-21 0.07598969712700665 1105 22-23 0.19218510654118148 1105 24-25 0.056084934785886276 1105 26-27 0.20904306029469666 1105 28-29 -0.02378034492476644 1105 30-31 -0.003403261747656927 1105 32-33 0.016177076927249345 1105 34-35 0.25523422052872036 1105 36-37 0.290920528415036 1105 38-39 0.452509255648188 1105 40-41 0.7354803661610205 1105 42-43 0.4159565063844397 1105 44-45 0.4041271576396568 1105 46-47 0.7781819455875478 1105 48-49 0.718893798806647 1105 50-51 0.9073635147111112 1105 52-53 0.8964242917846583 1105 54-55 1.043666336237834 1105 56-57 1.094605942695015 1105 58-59 1.04999650774457 1105 60-61 1.1424365955114908 1105 62-63 1.0632180272318976 1105 64-65 0.9269555197411137 1105 66-67 0.9786779797481095 1105 68-69 1.0118119253672297 1105 70-71 0.9877409493052447 1105 72-73 0.9658569359473219 1105 74-75 0.9006241221081908 1105 76-77 0.9777605045796065 1105 78-79 0.9930574093975224 1105 80-81 0.8002339202642759 1105 82-83 0.6469297579496995 1105 84-85 0.7207061495246805 1105 86-87 0.8725264132934747 1105 88-89 0.7371254648339303 1105 90-91 0.30476369774558165 1105 92-93 0.8867103318851299 1105 94-95 0.65991908687662 1105 96-97 0.6166734455091643 1105 98-99 0.6244787470654813 1105 100-101 0.21714181712621894 1106 1 0.214054267628768 1106 2 0.31926756418990365 1106 3 0.05745203529804144 1106 4 -0.3354681669214088 1106 5 -0.34044358147821896 1106 6 -0.44748612439818203 1106 7 -0.18593262054864823 1106 8 -0.3186943047841524 1106 9 -0.26187434430880074 1106 10-11 -0.432916814373165 1106 12-13 -0.3171518048679758 1106 14-15 -0.06762975408604177 1106 16-17 0.14846955976447163 1106 18-19 0.06675837530413986 1106 20-21 -0.03571342916372089 1106 22-23 -0.0974116973278214 1106 24-25 0.02415741847104158 1106 26-27 0.08104387134297752 1106 28-29 -0.12781230623475182 1106 30-31 -0.31953561281139997 1106 32-33 -0.071926797330228 1106 34-35 -0.3183120880751531 1106 36-37 0.21367193460753953 1106 38-39 0.062346219924513946 1106 40-41 0.1931669260396589 1106 42-43 0.07038986149591864 1106 44-45 0.08318108484055386 1106 46-47 0.4638444143284026 1106 48-49 0.5606755517222908 1106 50-51 0.13487006692985304 1106 52-53 0.7185869370036038 1106 54-55 0.4770101831599831 1106 56-57 0.27220298687461764 1106 58-59 -0.11213429631968452 1106 60-61 0.5276507347865014 1106 62-63 0.11263564950869309 1106 64-65 0.07676289577463535 1106 66-67 0.45222128446946286 1106 68-69 0.597208550505222 1106 70-71 0.23778037526329499 1106 72-73 0.06439554710224016 1106 74-75 -0.08152530602767527 1106 76-77 -0.08677791132294743 1106 78-79 -0.18682258549587516 1106 80-81 -0.10621015870779615 1106 82-83 0.1745391931446889 1106 84-85 0.7416582300888095 1106 86-87 0.4757923845613412 1106 88-89 -0.14933528568612076 1106 90-91 0.2225399235173917 1106 92-93 0.17030312557096394 1106 94-95 0.5060489597372921 1106 96-97 -0.024426426074857233 1106 98-99 -0.2657368566022562 1106 100-101 -0.0675736776507776 1107 1 0.4814778514823743 1107 2 0.44069920894811787 1107 3 0.39084683522428065 1107 4 0.2927643172948464 1107 5 0.03725985099195128 1107 6 0.24984359118710842 1107 7 0.20413437803762235 1107 8 0.06716662265892381 1107 9 0.21199796323490716 1107 10-11 0.4853765217700641 1107 12-13 0.36348973074314017 1107 14-15 0.7216232511913034 1107 16-17 0.42830061444339407 1107 18-19 0.5813566911194883 1107 20-21 0.5433852949654252 1107 22-23 0.36051441982750276 1107 24-25 0.48114397484908267 1107 26-27 0.4871448697360634 1107 28-29 0.7678481523060228 1107 30-31 0.9653664357823288 1107 32-33 0.8793301944947132 1107 34-35 0.6586774538231097 1107 36-37 0.6313989000018481 1107 38-39 0.7600008303580239 1107 40-41 0.9022674941666011 1107 42-43 0.8866003582337996 1107 44-45 0.9076149336151786 1107 46-47 1.294091246603628 1107 48-49 1.1399103804500186 1107 50-51 0.698874352029474 1107 52-53 0.7164970728447777 1107 54-55 0.8263862972419815 1107 56-57 0.46014587562886433 1107 58-59 0.7066270640238308 1107 60-61 0.7037920377026516 1107 62-63 0.6685614681119958 1107 64-65 0.4929795391718059 1107 66-67 0.675380140488361 1107 68-69 0.5771089746247107 1107 70-71 0.46486870275730396 1107 72-73 0.05336229367548029 1107 74-75 0.5405385665432973 1107 76-77 0.4683224987470389 1107 78-79 0.28190409012735174 1107 80-81 -0.03840811626072238 1107 82-83 0.057050191274349515 1107 84-85 0.3523507829720316 1107 86-87 0.29979157671468926 1107 88-89 0.504133625389283 1107 90-91 0.6740603435976489 1107 92-93 0.8698807622682772 1107 94-95 0.5765906560396381 1107 96-97 0.6476053960583812 1107 98-99 0.7785586045289037 1107 100-101 1.0645663256859805 1108 1 0.6562381587290709 1108 2 0.4723954820237033 1108 3 0.287930191472789 1108 4 0.1860070898479691 1108 5 0.2929290658263213 1108 6 0.11674307605392187 1108 7 0.12435289716721343 1108 8 0.1497070908618241 1108 9 0.3478116353462184 1108 10-11 0.24129322051798852 1108 12-13 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4 21.0 5 48.0 6 120.0 7 252.0 8 450.0 9 877.0 10 1425.0 11 2495.0 12 3643.0 13 4783.0 14 5503.0 15 7287.0 16 9941.0 17 12957.0 18 9999.0 19 4919.0 20 3726.0 21 3845.0 22 3886.0 23 4590.0 24 5039.0 25 5544.0 26 6404.0 27 7456.0 28 8678.0 29 10278.0 30 11809.0 31 13846.0 32 16353.0 33 19907.0 34 26031.0 35 33821.0 36 36439.0 37 36881.0 38 19283.0 39 1278.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.375433965853425 17.157702002187662 13.718373995339325 12.748490036619584 2 14.347475484713998 25.872308615960538 40.73245670830047 19.047759191024994 3 23.853777971348862 36.78563440961472 28.60169740915565 10.758890209880757 4 10.570105862928552 17.908110860294485 36.84865750973427 34.6731257670427 5 9.250485551174151 45.517038432111114 29.927608734035665 15.304867282679066 6 21.14501795067977 37.09934671296568 21.3889706315108 20.36666470484374 7 22.779706903654876 41.52168795244541 19.809605085045025 15.889000058854688 8 26.14354658348537 28.536872461891587 20.91254193396504 24.407039020657994 9 30.310467614255028 19.624496041905772 21.87281127689003 28.192225066949177 10-11 23.286151491966336 28.810105349891117 24.605967865340475 23.297775292802072 12-13 32.875883926443194 27.34897106989309 25.775927773314617 13.999217230349098 14-15 27.186745924312874 26.095580012948034 33.29998234359366 13.41769171914543 16-17 15.772173503619562 30.24954387616974 34.82476016714731 19.15352245306339 18-19 15.329733388264374 28.70534400565005 41.44414690130069 14.520775704784885 20-21 18.73447707609911 25.721411335412864 39.167500441410155 16.376611147077867 22-23 16.996231789804202 25.84747944846704 38.101004512729666 19.05528424899909 24-25 17.649343770231297 26.53787299158378 40.30486728267906 15.507915955505855 26-27 19.871991054087456 23.554676004943794 41.93867341533753 14.634659525631216 28-29 16.00670943440645 24.768848213760226 44.42528397386852 14.799158377964805 30-31 15.35680654464128 29.94497086692955 41.5753928550409 13.122829733388263 32-33 15.100139346059391 28.89245633112273 39.936698337117406 16.070705985700474 34-35 16.296322454012284 26.185851879971395 40.14990333089086 17.367922335125463 36-37 18.743240717988407 26.4316435189613 40.87542909945396 13.949686663596328 38-39 20.67888882349479 24.04346418692249 41.4353186981343 13.842328291448414 40-41 16.223736504988942 27.203381974476276 42.6490171467754 13.923864373759386 42-43 15.030402203791887 30.381602104903788 39.445167138543404 15.14282855276092 44-45 15.888491283415535 31.111761797699906 36.10113827645875 16.89860864242581 46-47 16.215953751101708 29.850932938214637 33.14994842759337 20.783164883090283 48-49 18.54961652334116 29.647189701753458 33.172643958256074 18.630549816649303 50-51 20.905455273116786 31.27232812858648 32.361440535816236 15.460776062480505 52-53 17.087048644830936 31.883552573496953 34.25664341838086 16.77275536329125 54-55 14.53700801537445 31.524686970712317 35.160520859544796 18.77778415436843 56-57 17.699449692475206 33.83022865719078 30.33033165592537 18.13998999440864 58-59 16.84438513138709 31.504208889209323 31.517598468585252 20.13380751081834 60-61 17.145148474057507 29.716736808028486 33.26937225933665 19.86874245857736 62-63 22.12647991030044 29.16324554665819 31.71679894997366 16.993475593067707 64-65 19.90640452083824 30.586296209088772 33.60931245585119 15.897986814221804 66-67 16.688933684504804 31.30953607109751 34.38150169945411 17.620028544943573 68-69 16.468031510892565 34.579585014375304 32.32029403888541 16.63208943584673 70-71 16.727827036316604 34.01521995662371 29.53295450198195 19.723998505077734 72-73 16.583632511329526 32.12465422870932 29.068183155788358 22.2235301041728 74-75 17.323435936672357 32.810164204578896 30.820581484315223 19.045818374433523 76-77 18.77875624416047 30.435160049144027 32.48743811016207 18.298645596533433 78-79 16.15940290864249 30.3737265675929 34.76008776254081 18.70678276122379 80-81 15.136193068942683 35.24891472297844 31.956000294312414 17.658891913766464 82-83 15.223798893697548 33.866864048996334 30.092074147868992 20.817262909437122 84-85 16.265388226222743 30.972897886629642 29.89781894057783 22.863894946569783 86-87 17.906801871202525 30.78448944395148 31.223889470144783 20.084819214701213 88-89 19.67279465333041 27.77462981142128 32.608090944012154 19.944484591236158 90-91 16.033884436176756 29.600757042823545 34.2738481734723 20.091510347527397 92-93 14.34303572847419 32.49537245485017 32.50758505413272 20.654006762542927 94-95 14.340812071144885 33.17210416571631 30.365627726904183 22.121456036234623 96-97 14.5292517146948 30.839784745286337 28.868529519532288 25.762434020486573 98-99 16.618964723125167 29.66742057810341 29.461701348064285 24.25191335070714 100-101 18.434052471956242 30.417783193022572 29.882733462534013 21.265430872487173 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3009.0 1 2153.5 2 3095.0 3 4072.5 4 2631.0 5 2036.0 6 2647.0 7 4056.5 8 4904.5 9 4780.0 10 3946.0 11 3063.5 12 2835.0 13 2697.5 14 2630.0 15 2525.5 16 2303.5 17 2249.5 18 2270.0 19 2326.5 20 2496.5 21 2638.5 22 2745.5 23 2887.0 24 3100.5 25 3353.0 26 3717.0 27 4137.5 28 4332.5 29 4590.5 30 5019.5 31 5233.0 32 5501.0 33 5902.5 34 6366.5 35 6592.5 36 6722.0 37 7033.0 38 6965.5 39 6615.5 40 6220.5 41 5994.0 42 5843.0 43 5789.5 44 7626.5 45 8260.5 46 8522.5 47 13835.0 48 35569.5 49 34039.0 50 13669.5 51 9378.0 52 4795.0 53 3115.5 54 2524.5 55 2200.5 56 2125.0 57 2090.0 58 2000.5 59 2111.0 60 2227.5 61 2150.0 62 1729.5 63 933.5 64 423.0 65 312.0 66 246.5 67 187.5 68 150.5 69 132.0 70 121.0 71 109.0 72 104.5 73 119.5 74 120.5 75 89.0 76 65.5 77 49.0 78 46.0 79 40.0 80 26.0 81 21.0 82 17.0 83 9.5 84 2.5 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.99699841092343 2 0.009416750044141017 3 0.0023541875110352543 4 0.012065210994055676 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0029427343887940675 10-11 0.0 12-13 2.942734388794068E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010299570360779236 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.005444058619269025 34-35 2.942734388794068E-4 36-37 0.002795597669354364 38-39 0.0 40-41 0.005444058619269025 42-43 0.012359484432935082 44-45 0.0023541875110352543 46-47 0.0019127773527161437 48-49 0.01000529692189983 50-51 5.885468777588136E-4 52-53 0.0029427343887940675 54-55 0.010446707080218939 56-57 0.0029427343887940675 58-59 0.0010299570360779236 60-61 0.008828203166382203 62-63 0.005591195338708728 64-65 0.017656406332764406 66-67 0.0014713671943970337 68-69 2.942734388794068E-4 70-71 8.828203166382202E-4 72-73 0.0 74-75 0.0 76-77 7.356835971985169E-4 78-79 0.011623800835736567 80-81 0.013242304749573304 82-83 0.011623800835736567 84-85 0.009122476605261609 86-87 0.01132952739685716 88-89 0.027808839974103938 90-91 0.02265905479371432 92-93 0.002059914072155847 94-95 0.010152433641339532 96-97 0.010740980519098345 98-99 0.010740980519098345 100-101 0.01132952739685716 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 339820.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.36675799639861 #Duplication Level Percentage of deduplicated Percentage of total 1 84.98290679197427 35.154673390941 2 8.68108823378521 7.182169522247526 3 2.469645766799331 3.064837163160546 4 1.0761109601675392 1.7806088666609095 5 0.6537653461570122 1.3522076430454446 6 0.36591558418767905 0.9082044850921527 7 0.26673880279684176 0.7723883652482233 8 0.19271047527763735 0.6377446075344785 9 0.14505317026247114 0.5400341451772244 >10 0.9406734841202342 7.542803266414346 >50 0.09527585337294371 2.790348419246106 >100 0.09669788103522645 8.250748799061007 >500 0.012087235129403306 3.182414872588054 >1k 0.017064331947392902 13.923212129258161 >5k 0.004266082986848226 12.917604324324838 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9549 2.810017067859455 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 8212 2.4165734800776884 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8028 2.3624271673238773 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6770 1.9922311812135836 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6236 1.8350891648519805 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5124 1.50785710081808 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4934 1.451945147430993 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4376 1.287740568536284 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3003 0.8837031369548585 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2892 0.8510387852392443 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2770 0.8151374256959567 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2362 0.6950738626331587 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2300 0.6768289094226355 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2236 0.6579954093343535 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2048 0.6026720028250251 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1820 0.5355776587605203 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1767 0.5199811664999117 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1570 0.46200929904066856 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1535 0.4517097286798894 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1513 0.44523571302454246 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1498 0.4408216114413513 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1321 0.3887352127596963 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1298 0.38196692366546997 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1288 0.37902418927667586 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1247 0.36695897828262025 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1162 0.34194573597787065 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1150 0.33841445471131776 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1145 0.3369430875169207 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1097 0.3228179624507092 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1006 0.29603907951268316 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 887 0.2610205402860338 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 846 0.2489553292919781 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 825 0.24277558707551058 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 750 0.22070507915955506 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 726 0.2136425166264493 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 703 0.20687422753222295 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 640 0.18833500088282032 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 628 0.18480371961626743 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 585 0.17214996174445293 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 579 0.1703843211111765 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 551 0.1621446648225531 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 525 0.15449355541168855 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 521 0.1533164616561709 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 520 0.1530221882172915 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 515 0.15155082102289447 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 513 0.15096227414513566 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 506 0.1489023600729798 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 488 0.1436054381731505 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 483 0.14213407097875347 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 481 0.14154552410099466 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 475 0.1397798834677182 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 459 0.1350715084456477 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 456 0.13418868812900947 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 443 0.1303631334235772 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 442 0.13006885998469778 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 440 0.12948031310693897 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 438 0.12889176622918017 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 411 0.12094638337943617 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 398 0.11712082867400388 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 390 0.11476664116296864 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 388 0.11417809428520982 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 383 0.11270672709081278 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 375 0.11035253957977753 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 360 0.10593843799658643 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 355 0.1044670708021894 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.414101583191101E-4 0.0 18-19 0.0 0.0 0.0 5.885468777588135E-4 0.0 20-21 0.0 0.0 0.0 0.0010299570360779236 0.0 22-23 0.0 0.0 0.0 0.0022070507915955507 0.0 24-25 0.0 0.0 0.0 0.004708375022070508 0.0 26-27 0.0 0.0 0.0 0.008092519569183686 0.0 28-29 0.0 0.0 0.0 0.011623800835736567 0.0 30-31 0.0 0.0 0.0 0.019422046966040846 0.0 32-33 0.0 0.0 0.0 0.025454652463068682 0.0 34-35 0.0 0.0 0.0 0.032811488435053854 0.0 36-37 0.0 0.0 0.0 0.04414101583191101 0.0 38-39 0.0 0.0 0.0 0.05385203931493143 0.0 40-41 0.0 0.0 0.0 0.06738861750338415 0.0 42-43 0.0 0.0 0.0 0.08828203166382202 0.0 44-45 0.0 0.0 0.0 0.11609087163792597 0.0 46-47 0.0 0.0 0.0 0.17715261020540285 0.0 48-49 0.0 0.0 0.0 0.29662762639044205 0.0 50-51 0.0 0.0 0.0 0.3821140603849097 0.0 52-53 0.0 0.0 0.0 0.4515625919604497 0.0 54-55 0.0 0.0 0.0 0.5473485963156965 0.0 56-57 0.0 0.0 0.0 0.6063504208110175 0.0 58-59 0.0 0.0 0.0 0.7061091165911364 0.0 60-61 0.0 0.0 0.0 0.8798775822494262 0.0 62-63 0.0 0.0 0.0 0.9930257194985581 0.0 64-65 0.0 0.0 0.0 1.0926372785592373 0.0 66-67 0.0 0.0 0.0 1.2758224942616678 0.0 68-69 0.0 0.0 0.0 1.4707786475192748 0.0 70-71 0.0 0.0 0.0 1.6483726678829969 0.0 72-73 0.0 0.0 0.0 1.8523041610264257 0.0 74-75 0.0 0.0 0.0 2.0210699782237658 0.0 76-77 0.0 0.0 0.0 2.124507091989877 0.0 78-79 0.0 0.0 0.0 2.2714966747101406 0.0 80-81 0.0 0.0 0.0 2.5026484609499144 0.0 82-83 0.0 0.0 0.0 2.7310046495203344 0.0 84-85 0.0 0.0 0.0 3.0989935848390324 0.0 86-87 0.0 0.0 0.0 3.755959037137308 0.0 88-89 0.0 0.0 0.0 4.339356129715732 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCG 25 5.029638E-5 76.718925 1 CGCCGGT 45 2.421075E-9 73.86348 7 TACCTGG 175 0.0 56.980404 2 GCCGGTC 60 2.3510438E-8 55.39761 8 TCTTGCG 75 1.3626777E-7 44.31809 2 CCGGTCC 75 1.3626777E-7 44.31809 9 ATAACGC 55 4.9659702E-5 43.16697 3 TATAACG 55 4.9659702E-5 43.16697 2 AGGCTTA 55 4.9659702E-5 43.16697 6 TACCCGG 45 9.63178E-4 42.207703 2 CTAGGAC 90 1.1959855E-8 42.207703 3 CTTGCGC 85 3.6404163E-7 39.104195 3 CGGACTT 25 0.0016139926 38.006508 82-83 GCGAACA 25 0.0016180829 37.98693 14-15 GCGCCGG 90 5.697566E-7 36.93174 6 TCTAGCG 40 1.0773259E-5 35.623234 28-29 TACCAGC 80 1.0779151E-5 35.612747 2 ACCTGGG 285 0.0 34.987965 3 GGACAAT 95 8.699335E-7 34.987965 6 TGGGAGT 235 0.0 34.349884 6 >>END_MODULE