FastQCFastQC Report
Thu 26 May 2016
SRR936633_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936633_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345691
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA91672.6517901825618835No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91002.6324087118264576No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA74652.15944297074555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69932.0229048485497163No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT60221.7420181607273548No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50441.4591065431266652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46881.3561244001145532No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35961.0402353546953782No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA32750.9473778605749064No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT32170.9305998709830456No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG32030.9265500114263895No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC25380.7341816824852253No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG22430.648845356112829No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18430.5331350830655123No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17160.49639707137298916No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG16980.4911901090858599No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16020.4634196435545039No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA14440.41771408570081375No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT14370.4156891559224857No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13880.4015146474741894No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT13760.39804333928276986No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG13240.38300100378661867No Hit
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT12930.37403345762545165No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA12730.3682479439730858No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG12700.3673801169252309No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT11930.34510588936362246No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10530.30460729379706153No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA9800.2834901689659262No Hit
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA9640.2788617580440335No Hit
GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT8020.2319990974598702No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA7890.22823851358583241No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7720.22332082698132147No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7570.2189816917420471No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC7540.2181138646941922No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7540.2181138646941922No Hit
CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA7410.2143532808201544No Hit
GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC7150.2068321130720788No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG6970.20162515078494958No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC6000.17356540957097522No Hit
TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT5710.16517641477504477No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5640.16315148499671672No Hit
GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5590.16170510658362527No Hit
GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT5450.15765524702696918No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5350.15476249020078625No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5110.14781987381794726No Hit
AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC5040.1457949440396192No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4860.14058798175248993No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4740.13711667356107043No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA4660.1348024681001241No Hit
ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT4630.1339346410522692No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4600.13306681400441436No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA4300.12438854352586559No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG4290.12409926784324729No Hit
GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA4170.12062795965182779No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4150.12004940828659122No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4090.11831375419088146No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG4070.11773520282564486No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.11513172168208025No Hit
GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC3820.11050331076018756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3690.10674272688614976No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3650.10558562415567661No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC3650.10558562415567661No Hit
CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT3550.10269286732949368No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC3510.1015357645990205No Hit
CTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG3470.10037866186854735No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGGG255.2572686E-576.037413
GTACGTG255.2572686E-576.037411
TACGTGG255.2572686E-576.037412
TCACGCA452.404704E-973.925264
TATCACG505.5351848E-966.532732
ATCACGC505.5351848E-966.532733
ATAGCGT301.295852E-463.373696
ATAACGC551.17543095E-860.4843033
TATAACG551.17543095E-860.4843032
TACCTGG2450.048.4932442
AATAGCG405.366037E-447.5302625
TAACGCA751.3538556E-744.3551564
TAGCGTA459.5856143E-442.2491237
TGGGACG459.5856143E-442.2491236
TTCAACG459.592449E-442.2430042
AGCGTAT459.599288E-442.236898
GTACCAG608.25473E-539.602821
ACCTGGG3000.039.6028183
CACGCAG853.6127494E-739.142575
GCTTATG500.00161035338.0187031
Produced by FastQC (version 0.11.5)