##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936633_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 345691 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.523403849102234 31.0 31.0 34.0 30.0 34.0 2 31.330532180473313 31.0 31.0 34.0 28.0 34.0 3 31.32384991220483 31.0 31.0 34.0 28.0 34.0 4 35.0160200873034 37.0 35.0 37.0 32.0 37.0 5 35.060290837771305 37.0 35.0 37.0 32.0 37.0 6 35.315724158280084 37.0 35.0 37.0 33.0 37.0 7 35.24138609336083 37.0 35.0 37.0 33.0 37.0 8 35.22745457648594 37.0 35.0 37.0 32.0 37.0 9 36.62409203595118 39.0 37.0 39.0 32.0 39.0 10-11 36.53182611060166 39.0 37.0 39.0 32.0 39.0 12-13 36.381554625373525 39.0 36.0 39.0 32.0 39.0 14-15 37.054210262922666 39.0 36.0 41.0 31.5 41.0 16-17 37.05336123879418 39.0 36.5 41.0 31.5 41.0 18-19 37.40918045306357 39.5 37.0 41.0 32.0 41.0 20-21 37.489079842981155 39.0 37.0 41.0 32.0 41.0 22-23 37.30873381140961 39.0 36.0 41.0 32.0 41.0 24-25 37.29228704247435 39.0 36.0 41.0 32.0 41.0 26-27 37.05109621019928 39.0 35.5 41.0 31.5 41.0 28-29 36.83382558411992 39.0 35.0 41.0 31.0 41.0 30-31 36.4105979039084 38.0 35.0 40.0 31.0 41.0 32-33 35.88940412102137 38.0 35.0 40.0 30.0 41.0 34-35 35.501761688907145 38.0 35.0 40.0 30.0 41.0 36-37 35.049156616747325 37.0 35.0 40.0 29.0 41.0 38-39 34.59433713923706 36.5 34.5 40.0 26.0 41.0 40-41 34.17194257299148 36.0 34.0 40.0 24.5 41.0 42-43 33.77719553011215 36.0 33.5 40.0 23.0 41.0 44-45 33.10080968263565 36.0 33.0 40.0 15.5 41.0 46-47 31.962026781142697 36.0 32.5 40.0 2.0 41.0 48-49 31.16281158606964 36.0 31.0 40.0 2.0 41.0 50-51 30.047527994654185 35.0 29.0 39.0 2.0 40.0 52-53 30.00294771920588 35.0 29.0 39.0 2.0 40.0 54-55 30.332895562800303 35.0 30.0 39.0 2.0 41.0 56-57 30.307074815369795 35.0 30.0 40.0 2.0 41.0 58-59 30.09731089325438 35.0 29.5 39.5 2.0 41.0 60-61 29.665163397369327 35.0 28.0 39.0 2.0 41.0 62-63 29.186111295926132 35.0 27.5 39.0 2.0 40.5 64-65 28.621938378494086 35.0 26.0 38.0 2.0 40.0 66-67 28.201322857696614 35.0 26.0 37.5 2.0 40.0 68-69 27.915287062723646 35.0 26.0 37.0 2.0 39.0 70-71 27.388293302400122 34.5 24.0 36.5 2.0 39.0 72-73 26.80360495355679 34.0 22.0 35.5 2.0 38.5 74-75 26.359125635321718 34.0 20.0 35.0 2.0 37.5 76-77 25.801409351125717 33.0 16.0 35.0 2.0 37.0 78-79 25.34006670697241 33.0 10.0 35.0 2.0 36.0 80-81 24.904526007330247 33.0 4.5 35.0 2.0 36.0 82-83 24.436958150486994 33.0 2.0 35.0 2.0 36.0 84-85 24.059618271809217 32.5 2.0 35.0 2.0 35.0 86-87 23.5020437326977 32.0 2.0 35.0 2.0 35.0 88-89 22.8506715534972 31.0 2.0 35.0 2.0 35.0 90-91 22.233465725170745 31.0 2.0 35.0 2.0 35.0 92-93 21.961430873236502 31.0 2.0 35.0 2.0 35.0 94-95 21.60116693810368 31.0 2.0 35.0 2.0 35.0 96-97 20.830733227072734 30.0 2.0 34.0 2.0 35.0 98-99 20.037223994839323 29.0 2.0 34.0 2.0 35.0 100-101 18.207358594814444 23.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.19083491927652574 1101 2 0.3639123895448826 1101 3 0.12447662304030516 1101 4 0.19881403415521248 1101 5 -0.13256682410941067 1101 6 -0.26420968603721207 1101 7 0.25635766310645636 1101 8 0.10551358918376508 1101 9 0.43547642775876483 1101 10-11 0.410351663300645 1101 12-13 0.6520266582700387 1101 14-15 0.5633243557557321 1101 16-17 0.6569183847514566 1101 18-19 0.6450950452872348 1101 20-21 0.43302072152907556 1101 22-23 0.4853708631458389 1101 24-25 0.40127715893562055 1101 26-27 0.08109643145650125 1101 28-29 0.206136795117267 1101 30-31 -0.00793220495449276 1101 32-33 -0.1291446866143815 1101 34-35 -0.054634466759900135 1101 36-37 -0.205165461467125 1101 38-39 -0.6094985352271038 1101 40-41 -0.5410823153131119 1101 42-43 -0.4355578232730082 1101 44-45 -0.2716295344986932 1101 46-47 -0.4138985799960686 1101 48-49 -0.5161064201401757 1101 50-51 -0.46490975057107065 1101 52-53 -0.9687673724209631 1101 54-55 -0.8354216515746877 1101 56-57 -0.795120292833353 1101 58-59 -0.5600742993310384 1101 60-61 -0.8054033449504132 1101 62-63 -0.6115825909730823 1101 64-65 -0.5417651501344167 1101 66-67 -0.5071503721684856 1101 68-69 -0.7691750229919592 1101 70-71 -0.8438211732243808 1101 72-73 -1.188489246878028 1101 74-75 -0.9929063865670393 1101 76-77 -0.42184686480867484 1101 78-79 -0.6235815525917587 1101 80-81 -0.7030603765056114 1101 82-83 -0.7873917473578977 1101 84-85 -0.49111408892384034 1101 86-87 -0.5810538271514858 1101 88-89 -0.3317758728678619 1101 90-91 -0.7355256146460789 1101 92-93 -0.40193821399912366 1101 94-95 -0.42271996314761395 1101 96-97 -0.11487881117973942 1101 98-99 -0.46772517737406005 1101 100-101 0.15005233679561059 1102 1 0.40999458314206905 1102 2 0.5255370394048242 1102 3 0.4869416090347052 1102 4 0.5360689361159956 1102 5 0.4994219714088004 1102 6 0.3611684652232938 1102 7 0.493500520249313 1102 8 0.5251214323210149 1102 9 0.5297061196355202 1102 10-11 0.7136569854294947 1102 12-13 0.7146597114913291 1102 14-15 0.9218957843271625 1102 16-17 0.6117643231267991 1102 18-19 0.8883443450071269 1102 20-21 0.7194632985598872 1102 22-23 0.8190403309329568 1102 24-25 0.4900446659384201 1102 26-27 0.6616286443416612 1102 28-29 0.8363888959576045 1102 30-31 0.7379221367821955 1102 32-33 0.9496228203884272 1102 34-35 0.6481946648927561 1102 36-37 1.0824535861519209 1102 38-39 0.6983726132322801 1102 40-41 0.6970969563955762 1102 42-43 1.2992601038978506 1102 44-45 1.4273340509354782 1102 46-47 1.9131042211243816 1102 48-49 2.2174229916245274 1102 50-51 2.367079044947136 1102 52-53 2.196666801248501 1102 54-55 2.33334585542811 1102 56-57 2.318745253385135 1102 58-59 2.644211414954672 1102 60-61 2.577929988382923 1102 62-63 2.3137955602874207 1102 64-65 2.042576586560262 1102 66-67 2.351813213265686 1102 68-69 1.718920215103278 1102 70-71 1.8241900312574124 1102 72-73 1.9927432461191685 1102 74-75 1.9084941736570507 1102 76-77 2.096752574967237 1102 78-79 2.3128890356435363 1102 80-81 2.61884438539915 1102 82-83 2.703196487936218 1102 84-85 2.2934797486111727 1102 86-87 2.0181898703275074 1102 88-89 2.403378188756786 1102 90-91 2.51256962344916 1102 92-93 2.627277472275388 1102 94-95 2.9748710732669537 1102 96-97 3.0038606846185765 1102 98-99 3.333983506099333 1102 100-101 2.7389318886163387 1103 1 0.30448150467124435 1103 2 0.44364569131629494 1103 3 0.5098792347892314 1103 4 0.6588053546270629 1103 5 0.6667861565194642 1103 6 0.4737237972152428 1103 7 0.32919468523925133 1103 8 0.14926630153630782 1103 9 0.43264374539004535 1103 10-11 0.4533350538399574 1103 12-13 0.36463959076697705 1103 14-15 0.2411915589750535 1103 16-17 0.688866938820972 1103 18-19 0.47900832891054534 1103 20-21 0.6338294957429866 1103 22-23 0.706525240389702 1103 24-25 0.21622172831266795 1103 26-27 0.2689727087279721 1103 28-29 0.15832048549483346 1103 30-31 -0.0016553280065352283 1103 32-33 0.24817412823550455 1103 34-35 0.1787580451744475 1103 36-37 0.16136706703723291 1103 38-39 0.5448313657473705 1103 40-41 0.12540681555049815 1103 42-43 0.2770669449441314 1103 44-45 0.0015191615994538665 1103 46-47 0.1494422492933971 1103 48-49 0.27180930953197446 1103 50-51 0.5577832702992502 1103 52-53 0.7607311875664102 1103 54-55 0.8754987729331454 1103 56-57 0.8198518932241683 1103 58-59 0.8592415960411905 1103 60-61 0.7438253606163201 1103 62-63 0.7376184979131786 1103 64-65 0.8441661237836016 1103 66-67 0.6820949034065329 1103 68-69 0.8789805772763195 1103 70-71 0.7813731298489621 1103 72-73 0.8442523004451274 1103 74-75 0.5678503104779757 1103 76-77 0.4797706836191473 1103 78-79 0.8671747499292515 1103 80-81 0.6031099789605179 1103 82-83 0.6198162062460746 1103 84-85 0.8480069115890423 1103 86-87 0.9460973149955123 1103 88-89 1.3254103819157415 1103 90-91 1.0691692210346737 1103 92-93 1.1058891422955064 1103 94-95 1.216842904252868 1103 96-97 1.7531765799908108 1103 98-99 1.843218918574177 1103 100-101 2.1020506008899815 1104 1 0.10347167209085839 1104 2 0.26920280759889437 1104 3 0.22494699987028355 1104 4 0.3091147582184206 1104 5 0.33537884472416124 1104 6 0.19734097196183598 1104 7 0.10002343130052793 1104 8 -0.07703490191078544 1104 9 0.3035066587190798 1104 10-11 0.23026075901439924 1104 12-13 0.5500235928929484 1104 14-15 0.714267751982149 1104 16-17 0.32432497002707095 1104 18-19 -0.1575316657745418 1104 20-21 0.13245521230651747 1104 22-23 0.3126791449491293 1104 24-25 0.5599368492268368 1104 26-27 0.27416233706402693 1104 28-29 0.37142932722445465 1104 30-31 0.5052806273482346 1104 32-33 0.34353117618356777 1104 34-35 0.40903024440758884 1104 36-37 0.39231881526243484 1104 38-39 0.8217418854694785 1104 40-41 0.8639702717594488 1104 42-43 0.753464955649882 1104 44-45 0.7694095226335875 1104 46-47 0.9273360270542987 1104 48-49 0.7130833689830212 1104 50-51 0.3857852444080514 1104 52-53 0.2945643753724916 1104 54-55 0.6237771222744719 1104 56-57 0.6908476792611431 1104 58-59 0.23507394864739695 1104 60-61 0.4538059991645902 1104 62-63 0.5763831613655874 1104 64-65 0.42713184262494863 1104 66-67 1.024454722699648 1104 68-69 1.0336102420574598 1104 70-71 0.9725997347614559 1104 72-73 1.0126893377633763 1104 74-75 0.7335615591017977 1104 76-77 0.6427903108162916 1104 78-79 0.39655480383760633 1104 80-81 0.7084400727306885 1104 82-83 0.960824520281232 1104 84-85 1.0042614197702022 1104 86-87 0.8543354228881554 1104 88-89 0.6450223936085386 1104 90-91 0.7424618067375697 1104 92-93 0.9782478226797018 1104 94-95 0.3244128522426948 1104 96-97 0.7109496334056402 1104 98-99 1.1961667945090362 1104 100-101 1.7559399748697118 1105 1 0.09570886885635588 1105 2 -0.16589153202374618 1105 3 -0.07877267667958066 1105 4 -0.06678820674115116 1105 5 0.03370768569451599 1105 6 -0.07311724906242034 1105 7 -0.140785194036404 1105 8 0.08226428946387188 1105 9 -0.02172245179305321 1105 10-11 0.07508555685806328 1105 12-13 -0.3139117170801029 1105 14-15 -0.38096135852998003 1105 16-17 -0.08109281973033688 1105 18-19 -0.4687985121357343 1105 20-21 -0.40196527286868644 1105 22-23 -0.46095966906704433 1105 24-25 -0.4113839054901476 1105 26-27 -0.4397999270869093 1105 28-29 -0.7093253897566782 1105 30-31 -0.5863635775035192 1105 32-33 -0.6660914653258629 1105 34-35 -0.9403767636786711 1105 36-37 -0.8618321281337913 1105 38-39 -0.8616273867677222 1105 40-41 -0.6071887578901496 1105 42-43 -0.67931132467357 1105 44-45 -0.34838023477880853 1105 46-47 -0.275467207447047 1105 48-49 0.18313727733881535 1105 50-51 0.3899361878042775 1105 52-53 0.42523822981993575 1105 54-55 0.32620299828525745 1105 56-57 0.2444595390994202 1105 58-59 0.23278284352610257 1105 60-61 0.28935855981149317 1105 62-63 0.43093841743027994 1105 64-65 1.0054337294174012 1105 66-67 1.0260989275513985 1105 68-69 1.2360630722461359 1105 70-71 1.3941900312574127 1105 72-73 1.1313146746906 1105 74-75 1.219922745085622 1105 76-77 0.9896097178243757 1105 78-79 1.1286033213578186 1105 80-81 1.4488443853991484 1105 82-83 1.5103393450790783 1105 84-85 1.4377654628968877 1105 86-87 1.5267612988989328 1105 88-89 1.5876639030425004 1105 90-91 1.5611410520205915 1105 92-93 1.8072774722753877 1105 94-95 2.1391567875526647 1105 96-97 1.682432113190007 1105 98-99 1.786840648956474 1105 100-101 1.5960747457591964 1106 1 0.1378841935316828 1106 2 0.08056950693729448 1106 3 0.5649448557879495 1106 4 0.35717283221988794 1106 5 0.4794382051750361 1106 6 0.17873340028823037 1106 7 0.563581689080479 1106 8 0.5553162375158252 1106 9 0.8956639118433145 1106 10-11 0.5648907516632562 1106 12-13 0.6873788673354824 1106 14-15 0.8200207843271627 1106 16-17 0.8851977646852447 1106 18-19 0.966396293059077 1106 20-21 0.7894632985598875 1106 22-23 0.6395598114524361 1106 24-25 0.3807183672371224 1106 26-27 0.4816367612247774 1106 28-29 0.6267703894641059 1106 30-31 0.6435471367821961 1106 32-33 0.16665204116763732 1106 34-35 0.3264333012564009 1106 36-37 0.3361630017363382 1106 38-39 0.5949554054400679 1106 40-41 0.18976741094102323 1106 42-43 0.6545117272744747 1106 44-45 0.6535678171692396 1106 46-47 0.5220814938516547 1106 48-49 0.30782883578037357 1106 50-51 0.4161699540380468 1106 52-53 0.9387041389108433 1106 54-55 0.6594416346488927 1106 56-57 0.176578045592926 1106 58-59 0.38919518118843754 1106 60-61 0.25337641695434954 1106 62-63 0.31147413171599325 1106 64-65 0.1855473657810407 1106 66-67 -0.05131178673431336 1106 68-69 0.24002411120717326 1106 70-71 0.39867866762104853 1106 72-73 0.7498861032620283 1106 74-75 0.8790298879427674 1106 76-77 0.9984246528893124 1106 78-79 0.9944474772019767 1106 80-81 1.1164742555290204 1106 82-83 1.259901033390765 1106 84-85 0.9938855927670183 1106 86-87 1.2970616235742618 1106 88-89 1.4153911757697735 1106 90-91 1.8075615065660422 1106 92-93 1.9658083164312323 1106 94-95 1.7878256187214987 1106 96-97 2.109266528774423 1106 98-99 2.27861824635907 1106 100-101 2.525474096408548 1107 1 0.08071290436886613 1107 2 0.21125132511911104 1107 3 -0.11217057821307463 1107 4 -0.05698998236665176 1107 5 0.4201322216105723 1107 6 0.09722980501344836 1107 7 0.025526347529378768 1107 8 -0.11539511328027174 1107 9 0.3196334804103387 1107 10-11 0.18192978607517318 1107 12-13 0.5347000666164305 1107 14-15 0.2856810950616264 1107 16-17 0.1400048396724074 1107 18-19 0.1763346597770976 1107 20-21 0.2442655584468909 1107 22-23 0.10476268767226316 1107 24-25 0.0664127046793439 1107 26-27 0.1468990236798362 1107 28-29 -0.1680501678034929 1107 30-31 0.4245323062737256 1107 32-33 0.5182346040849524 1107 34-35 0.19616076658767412 1107 36-37 0.15056496629720328 1107 38-39 -0.1767847717556137 1107 40-41 0.10059978125432423 1107 42-43 0.4760881910649246 1107 44-45 0.9464543092082778 1107 46-47 1.1326522437232462 1107 48-49 1.1147272692677852 1107 50-51 0.6271355421222786 1107 52-53 0.7740517891419678 1107 54-55 0.8504967835959896 1107 56-57 0.8156782638774658 1107 58-59 0.9294333681427283 1107 60-61 0.26017373333852944 1107 62-63 0.4518343012075192 1107 64-65 0.7478066107733348 1107 66-67 0.3622651906668146 1107 68-69 0.4628669463381456 1107 70-71 0.5443595227828375 1107 72-73 0.7103380806631598 1107 74-75 0.45441830602186073 1107 76-77 0.38002134010040933 1107 78-79 0.5581352019066514 1107 80-81 0.1466167824935809 1107 82-83 -0.0018317606513527096 1107 84-85 -0.03537416583596098 1107 86-87 0.06430770729280155 1107 88-89 -0.08968072972585972 1107 90-91 -0.2849606428946636 1107 92-93 -0.5695425438666639 1107 94-95 0.20458858739124608 1107 96-97 0.6654587475725755 1107 98-99 0.9145565488757619 1107 100-101 0.9500214769940634 1108 1 0.14497050609552176 1108 2 0.4865322239955141 1108 3 0.23517595895444643 1108 4 0.33687150433429025 1108 5 0.5131779906704352 1108 6 0.4997880478878187 1108 7 0.6704828637485107 1108 8 0.440530742112351 1108 9 0.4972663122518881 1108 10-11 0.32574366279706624 1108 12-13 0.4005906906245542 1108 14-15 0.22644634612491643 1108 16-17 0.45093767786195826 1108 18-19 0.4569478763072823 1108 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-1.8476812229421746 2316 78-79 -2.2755081769000185 2316 80-81 -2.3638472522315084 2316 82-83 -2.5027791218198807 2316 84-85 -2.1180185092285484 2316 86-87 -2.17934497287807 2316 88-89 -2.2247141457379875 2316 90-91 -2.20605406993063 2316 92-93 -2.2150744441009174 2316 94-95 -3.147872829172073 2316 96-97 -4.321235134196751 2316 98-99 -5.5581680618449205 2316 100-101 -5.479029783857527 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 769.0 3 234.0 4 212.0 5 250.0 6 287.0 7 354.0 8 455.0 9 662.0 10 1050.0 11 1845.0 12 3193.0 13 4412.0 14 4730.0 15 6411.0 16 8926.0 17 12239.0 18 9138.0 19 4447.0 20 4027.0 21 4000.0 22 4209.0 23 4539.0 24 5082.0 25 5478.0 26 6237.0 27 7036.0 28 8300.0 29 9283.0 30 10904.0 31 13009.0 32 15669.0 33 19390.0 34 25672.0 35 37420.0 36 38614.0 37 38475.0 38 24840.0 39 3874.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 58.10248085031267 15.77349689061745 12.703469949387527 13.420552309682348 2 14.469203102982323 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34.93104914646741 17.219343845915745 46-47 15.916717290057566 30.436228991292776 32.11533454829472 21.531719170354943 48-49 17.993352348663237 30.46333956249313 32.167631897155225 19.37567619168841 50-51 20.755565712434336 31.46175163731457 31.910566753835827 15.872115896415265 52-53 16.820326489757782 31.946730618467527 33.73698568316665 17.495957208608033 54-55 14.705775993126263 31.699470728034633 34.96507441485722 18.629678863981876 56-57 17.47452872904759 34.3643357361606 30.00467256596202 18.15646296882979 58-59 16.321328966706396 31.80424241722889 31.45229884392395 20.422129772140764 60-61 16.85155959560307 29.6815714138993 33.04522921274524 20.421639777752382 62-63 21.993982613223785 29.347200324013134 30.90723677549072 17.751580287272358 64-65 20.019612891281522 30.682748466115694 33.457191538107736 15.840447104495055 66-67 16.988634802067345 31.369219219436413 34.091832882264534 17.550313096231708 68-69 16.13060957597168 34.98107055179667 32.22664415675348 16.661675715478168 70-71 16.146963358964122 34.06436047419853 29.547150816655964 20.241525350181384 72-73 16.09688010761242 32.438347050060024 28.81076702778541 22.654005814542142 74-75 16.92379123009008 32.680180049872426 30.973987653392427 19.422041066645065 76-77 18.856721318589127 30.273741764242168 32.19868517165814 18.670851745510564 78-79 16.104699349534464 30.484659745917774 34.74819592363358 18.66244498091418 80-81 15.165725956934683 35.02043910073743 32.241271071946215 17.572563870381675 82-83 14.808321797781412 34.16071085036183 29.910999058351813 21.119968293504943 84-85 15.944928592921787 31.173761081406383 30.13158808416082 22.749722241511005 86-87 18.0561341387211 30.885781897289917 31.58630146497258 19.471782499016406 88-89 19.739145025182907 27.927856256382515 32.44862889857697 19.88436981985761 90-91 16.30575809603432 29.547975937178624 34.6926407051639 19.453625261623156 92-93 14.843099018609388 32.67391329506527 32.71441880381446 19.768568882510877 94-95 14.393316939100245 33.566613626500796 31.186315637205265 20.853753797193693 96-97 14.571547186296222 31.144358633498864 29.205584156525337 25.078510023679577 98-99 16.42186186672762 30.506444300096497 29.938906096514767 23.13278773666112 100-101 18.838065361898174 30.570853096341228 30.884842556713444 19.706238985047158 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1272.0 1 1013.5 2 2361.0 3 3253.0 4 2023.0 5 1678.5 6 2428.0 7 4125.0 8 5604.5 9 5684.0 10 4433.0 11 3181.5 12 2959.5 13 3009.0 14 2920.0 15 2700.0 16 2448.0 17 2363.5 18 2395.0 19 2361.0 20 2395.5 21 2513.0 22 2653.0 23 2848.5 24 3095.0 25 3402.5 26 3718.0 27 4077.0 28 4322.5 29 4643.0 30 5080.0 31 5363.5 32 5694.0 33 6144.0 34 6485.0 35 6653.0 36 6974.0 37 7352.5 38 7163.5 39 6784.5 40 6473.0 41 6038.0 42 5800.0 43 5935.5 44 7883.0 45 8651.5 46 8980.0 47 14225.5 48 35487.0 49 34362.0 50 14312.0 51 9684.0 52 5271.0 53 3469.5 54 2775.5 55 2419.5 56 2255.0 57 2095.5 58 2027.0 59 2162.0 60 2314.0 61 2395.0 62 2064.5 63 1140.5 64 478.0 65 339.5 66 275.0 67 197.0 68 149.5 69 136.5 70 141.5 71 129.5 72 109.0 73 146.5 74 147.5 75 82.5 76 54.5 77 41.0 78 31.5 79 25.0 80 17.5 81 11.5 82 5.0 83 4.5 84 4.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03587018464466822 2 0.0254562600704097 3 0.02285277892684507 4 0.056408758110566955 5 0.06392992585864254 6 0.05264817423652915 7 0.05496237969747549 8 0.0439699037579804 9 0.04888759036249136 10-11 0.056987309475803535 12-13 0.048742952521182215 14-15 0.04136642261441577 16-17 0.05149107150605599 18-19 0.053516001284384024 20-21 0.011860302987349974 22-23 0.02155103835506276 24-25 0.006364065017602425 26-27 0.010847838098185951 28-29 0.007376529906766447 30-31 0.009690735367712785 32-33 0.0036159460327286506 34-35 0.0091121840024762 36-37 0.010269286732949369 38-39 0.013017405717823144 40-41 0.0026034811435646284 42-43 0.0020249297783280443 44-45 0.0028927568261829205 46-47 2.8927568261829205E-4 48-49 0.0014463784130914602 50-51 8.678270478548763E-4 52-53 0.004049859556656089 54-55 0.007955081272003032 56-57 0.0163440760679335 58-59 0.01128175162211339 60-61 0.011715665146040829 62-63 0.007521167748075594 64-65 0.0 66-67 0.008822908319857907 68-69 0.01894755721149813 70-71 0.003760583874037797 72-73 0.0017356540957097525 74-75 0.0026034811435646284 76-77 0.005351600128438403 78-79 0.004483773080583528 80-81 0.008678270478548761 82-83 0.005930151493674987 84-85 0.01938147073542557 86-87 0.00549623796974755 88-89 0.005785513652365841 90-91 0.0036159460327286506 92-93 0.017067265274479233 94-95 0.011860302987349974 96-97 0.009980011050331077 98-99 0.021840314037681052 100-101 0.039920044201324306 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 345691.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.24391400094595 #Duplication Level Percentage of deduplicated Percentage of total 1 85.23238830252646 36.85782069849686 2 8.653768798797703 7.484456674385544 3 2.461835570579873 3.193782170958772 4 1.0960701001092574 1.8959344459253176 5 0.5620987398423245 1.2153674782890787 6 0.3807058412133106 0.9877926394251711 7 0.24242234831812928 0.7338303827803584 8 0.17382145326254045 0.6013375981123796 9 0.14023387926924974 0.5457835633624625 >10 0.8453254373519962 6.922537989460517 >50 0.09191157281237052 2.781941187305701 >100 0.08855713584841539 7.890918589376135 >500 0.012746860463029488 3.8046070216213335 >1k 0.01408863524861154 12.379333659644828 >5k 0.004025324356746154 12.704555900855558 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9167 2.6517901825618835 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9100 2.6324087118264576 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7465 2.15944297074555 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6993 2.0229048485497163 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6022 1.7420181607273548 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5044 1.4591065431266652 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4688 1.3561244001145532 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3596 1.0402353546953782 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3275 0.9473778605749064 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3217 0.9305998709830456 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3203 0.9265500114263895 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2538 0.7341816824852253 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2243 0.648845356112829 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1843 0.5331350830655123 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1716 0.49639707137298916 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1698 0.4911901090858599 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1602 0.4634196435545039 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1444 0.41771408570081375 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1437 0.4156891559224857 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1388 0.4015146474741894 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1376 0.39804333928276986 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1324 0.38300100378661867 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1293 0.37403345762545165 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1273 0.3682479439730858 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1270 0.3673801169252309 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1193 0.34510588936362246 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1053 0.30460729379706153 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 980 0.2834901689659262 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 964 0.2788617580440335 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 802 0.2319990974598702 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 789 0.22823851358583241 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 772 0.22332082698132147 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 757 0.2189816917420471 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 754 0.2181138646941922 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 754 0.2181138646941922 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 741 0.2143532808201544 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 715 0.2068321130720788 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 697 0.20162515078494958 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 600 0.17356540957097522 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 571 0.16517641477504477 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 564 0.16315148499671672 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 559 0.16170510658362527 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 545 0.15765524702696918 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 535 0.15476249020078625 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 511 0.14781987381794726 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 504 0.1457949440396192 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 486 0.14058798175248993 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 474 0.13711667356107043 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 466 0.1348024681001241 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 463 0.1339346410522692 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 460 0.13306681400441436 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 430 0.12438854352586559 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 429 0.12409926784324729 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 417 0.12062795965182779 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 415 0.12004940828659122 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 409 0.11831375419088146 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 407 0.11773520282564486 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 398 0.11513172168208025 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 382 0.11050331076018756 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 369 0.10674272688614976 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 365 0.10558562415567661 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 365 0.10558562415567661 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 355 0.10269286732949368 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 351 0.1015357645990205 No Hit CTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG 347 0.10037866186854735 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.8927568261829205E-4 0.0 5 0.0 0.0 0.0 2.8927568261829205E-4 0.0 6 0.0 0.0 0.0 2.8927568261829205E-4 0.0 7 0.0 0.0 0.0 2.8927568261829205E-4 0.0 8 0.0 0.0 0.0 2.8927568261829205E-4 0.0 9 0.0 0.0 0.0 2.8927568261829205E-4 0.0 10-11 0.0 0.0 0.0 5.785513652365841E-4 0.0 12-13 0.0 0.0 0.0 8.678270478548761E-4 0.0 14-15 0.0 0.0 0.0 8.678270478548761E-4 0.0 16-17 0.0 0.0 0.0 8.678270478548761E-4 0.0 18-19 0.0 0.0 0.0 8.678270478548761E-4 0.0 20-21 0.0 0.0 0.0 8.678270478548761E-4 0.0 22-23 0.0 0.0 0.0 0.0013017405717823142 0.0 24-25 0.0 0.0 0.0 0.0021695676196371904 0.0 26-27 0.0 0.0 0.0 0.0036159460327286506 0.0 28-29 0.0 0.0 0.0 0.008099719113312177 0.0 30-31 0.0 0.0 0.0 0.01591016254400606 0.0 32-33 0.0 0.0 0.0 0.025022346546482263 0.0 34-35 0.0 0.0 0.0 0.034134530548958465 0.0 36-37 0.0 0.0 0.0 0.04801976331463648 0.0 38-39 0.0 0.0 0.0 0.057131947317112686 0.0 40-41 0.0 0.0 0.0 0.06986007735231753 0.0 42-43 0.0 0.0 0.0 0.09242358059654432 0.0 44-45 0.0 0.0 0.0 0.12684738682812108 0.0 46-47 0.0 0.0 0.0 0.1991663074826941 0.0 48-49 0.0 0.0 0.0 0.32789398624783406 0.0 50-51 0.0 0.0 0.0 0.410048280111429 0.0 52-53 0.0 0.0 0.0 0.48656169816396727 0.0 54-55 0.0 0.0 0.0 0.5807209328562213 0.0 56-57 0.0 0.0 0.0 0.652750577828176 0.0 58-59 0.0 0.0 0.0 0.7500918450292313 0.0 60-61 0.0 0.0 0.0 0.9356621954288656 0.0 62-63 0.0 0.0 0.0 1.0557116037154568 0.0 64-65 0.0 0.0 0.0 1.159706211616733 0.0 66-67 0.0 0.0 0.0 1.3497603350969507 0.0 68-69 0.0 0.0 0.0 1.5712008701412534 0.0 70-71 0.0 0.0 0.0 1.7459233824427018 0.0 72-73 0.0 0.0 0.0 1.963314057930348 0.0 74-75 0.0 0.0 0.0 2.128635110546702 0.0 76-77 0.0 0.0 0.0 2.2398616105134357 0.0 78-79 0.0 0.0 0.0 2.390863516840184 0.0 80-81 0.0 0.0 0.0 2.635590744335259 0.0 82-83 0.0 0.0 0.0 2.8749663717018956 0.0 84-85 0.0 0.0 0.0 3.2582566511711324 0.0 86-87 0.0 0.0 0.0 3.9455756730721947 0.0 88-89 0.0 0.0 0.0 4.526730519452343 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTGGG 25 5.2572686E-5 76.03741 3 GTACGTG 25 5.2572686E-5 76.03741 1 TACGTGG 25 5.2572686E-5 76.03741 2 TCACGCA 45 2.404704E-9 73.92526 4 TATCACG 50 5.5351848E-9 66.53273 2 ATCACGC 50 5.5351848E-9 66.53273 3 ATAGCGT 30 1.295852E-4 63.37369 6 ATAACGC 55 1.17543095E-8 60.484303 3 TATAACG 55 1.17543095E-8 60.484303 2 TACCTGG 245 0.0 48.493244 2 AATAGCG 40 5.366037E-4 47.530262 5 TAACGCA 75 1.3538556E-7 44.355156 4 TAGCGTA 45 9.5856143E-4 42.249123 7 TGGGACG 45 9.5856143E-4 42.249123 6 TTCAACG 45 9.592449E-4 42.243004 2 AGCGTAT 45 9.599288E-4 42.23689 8 GTACCAG 60 8.25473E-5 39.60282 1 ACCTGGG 300 0.0 39.602818 3 CACGCAG 85 3.6127494E-7 39.14257 5 GCTTATG 50 0.001610353 38.018703 1 >>END_MODULE