##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936633_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 345691 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.626302102166385 31.0 31.0 34.0 30.0 34.0 2 31.589766583451695 31.0 31.0 34.0 30.0 34.0 3 31.65074011183398 31.0 31.0 34.0 30.0 34.0 4 35.47661350743872 37.0 35.0 37.0 33.0 37.0 5 35.29043857086242 37.0 35.0 37.0 33.0 37.0 6 35.52168265879065 37.0 35.0 37.0 33.0 37.0 7 35.477735897087285 37.0 35.0 37.0 33.0 37.0 8 35.540138447341704 37.0 35.0 37.0 33.0 37.0 9 37.01574527540491 39.0 37.0 39.0 33.0 39.0 10-11 36.87620157886668 39.0 37.0 39.0 32.5 39.0 12-13 36.72446346592766 39.0 36.0 39.0 32.0 39.0 14-15 37.33964147171896 39.0 36.0 41.0 32.0 41.0 16-17 37.40460121900773 39.0 36.0 40.0 32.0 41.0 18-19 37.88126101055567 39.5 37.0 41.0 33.0 41.0 20-21 38.003558090896206 40.0 37.0 41.0 33.0 41.0 22-23 37.839285662629344 40.0 36.5 41.0 33.0 41.0 24-25 37.62022297369616 39.0 36.0 41.0 33.0 41.0 26-27 37.09094827461519 39.0 35.0 41.0 31.5 41.0 28-29 36.79154794310526 38.5 35.0 40.5 31.0 41.0 30-31 36.397826382520805 38.0 35.0 40.0 31.0 41.0 32-33 35.821595008258825 38.0 35.0 40.0 30.0 41.0 34-35 35.425909265789386 37.0 35.0 40.0 30.0 41.0 36-37 34.849222282327275 37.0 34.0 40.0 26.5 41.0 38-39 34.41485170282131 36.5 33.5 40.0 25.0 41.0 40-41 33.90572216227787 36.0 33.0 40.0 23.5 41.0 42-43 33.43066495801163 36.0 33.0 40.0 20.5 41.0 44-45 32.72808085833881 36.0 33.0 40.0 11.0 41.0 46-47 31.575268086238864 35.5 31.5 40.0 2.0 41.0 48-49 30.63766340460122 35.0 30.5 40.0 2.0 41.0 50-51 30.109189999161103 35.0 28.5 40.0 2.0 41.0 52-53 29.575542898137353 35.0 27.5 39.0 2.0 41.0 54-55 29.346393166151273 35.0 27.0 39.0 2.0 40.0 56-57 29.150850036593376 35.0 26.5 39.0 2.0 40.0 58-59 28.913516695546022 35.0 26.0 39.0 2.0 40.0 60-61 28.487822940140184 35.0 25.5 38.5 2.0 40.0 62-63 27.903070950646676 34.5 23.5 38.0 2.0 40.0 64-65 27.26624644552505 34.0 22.0 37.0 2.0 39.5 66-67 26.816393831485343 33.0 21.5 36.0 2.0 39.0 68-69 26.713442062419908 33.0 21.5 36.0 2.0 39.0 70-71 26.280961899499843 33.0 19.5 36.0 2.0 38.5 72-73 25.75548539013165 33.0 16.5 35.0 2.0 37.5 74-75 25.21753242057213 33.0 10.5 35.0 2.0 37.0 76-77 23.71589078107327 30.5 7.0 33.5 2.0 35.5 78-79 24.288911195258194 32.0 2.0 35.0 2.0 36.0 80-81 24.135273987462792 32.0 2.0 35.0 2.0 36.0 82-83 23.80860219097402 32.0 2.0 35.0 2.0 35.0 84-85 23.397324489211464 31.5 2.0 35.0 2.0 35.0 86-87 22.781974364389008 31.0 2.0 34.0 2.0 35.0 88-89 22.09611907744199 30.5 2.0 34.0 2.0 35.0 90-91 21.397670173652195 29.0 2.0 34.0 2.0 35.0 92-93 20.976221538888776 29.0 2.0 34.0 2.0 35.0 94-95 20.482561015473358 29.0 2.0 34.0 2.0 35.0 96-97 19.579046026653863 28.0 2.0 34.0 2.0 35.0 98-99 18.60712167803038 25.5 2.0 33.5 2.0 35.0 100-101 16.15412897645585 17.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25145119971241314 1101 2 0.2634416367964185 1101 3 0.23249158584738794 1101 4 -0.07284017507200957 1101 5 0.2858066685255878 1101 6 0.08621855770357456 1101 7 0.22183482740983607 1101 8 0.12912431381613487 1101 9 0.13040968361779903 1101 10-11 0.057433980903546455 1101 12-13 0.3135803019905339 1101 14-15 0.5677695495199515 1101 16-17 0.3894264250782271 1101 18-19 0.1262806373748262 1101 20-21 0.07984253769077299 1101 22-23 0.16187462117011364 1101 24-25 0.09951482848047277 1101 26-27 0.1168747792392466 1101 28-29 -0.13351120663664773 1101 30-31 -0.07279353968848312 1101 32-33 0.5622234090640887 1101 34-35 0.5901674985615841 1101 36-37 0.5494330606260434 1101 38-39 1.2161601037113385 1101 40-41 1.3089784383850613 1101 42-43 0.8501594564971313 1101 44-45 0.9282926062481778 1101 46-47 0.9579145567787855 1101 48-49 0.9380329653650641 1101 50-51 1.3047364936204495 1101 52-53 1.533266090140252 1101 54-55 1.7739655641574892 1101 56-57 1.5267879455747888 1101 58-59 1.3186208875596463 1101 60-61 1.0081232776667406 1101 62-63 1.2939338573811234 1101 64-65 1.078598643738129 1101 66-67 0.7393500603621312 1101 68-69 0.8454521010475098 1101 70-71 0.990184094598785 1101 72-73 0.6992231014371306 1101 74-75 0.633180518871562 1101 76-77 0.3776720580990194 1101 78-79 0.07196607941985178 1101 80-81 0.5005591428213165 1101 82-83 0.4260736865701453 1101 84-85 0.575847339637523 1101 86-87 0.37090598704758904 1101 88-89 0.37902095659485724 1101 90-91 0.38572393284799844 1101 92-93 0.28024501376754074 1101 94-95 0.9032739791934006 1101 96-97 1.1126377556596125 1101 98-99 1.164048054974213 1101 100-101 1.02185238040148 1102 1 0.13705344060877067 1102 2 0.4117329533230283 1102 3 0.18991455503506316 1102 4 0.18368643557224829 1102 5 0.2088879010185849 1102 6 0.14986001428621165 1102 7 0.39791325878238837 1102 8 0.22509070037075674 1102 9 0.5416141654105147 1102 10-11 0.37578131983912044 1102 12-13 0.5012833832230257 1102 14-15 0.601102882853283 1102 16-17 0.5356169012687033 1102 18-19 0.3322470239294546 1102 20-21 0.3099265713042172 1102 22-23 0.13223876682837954 1102 24-25 0.07805824584742282 1102 26-27 -0.196822699752353 1102 28-29 -0.27057003016605563 1102 30-31 0.10785071801460333 1102 32-33 0.8203186471593256 1102 34-35 0.489887386516763 1102 36-37 0.8462397833151201 1102 38-39 0.7047315322827714 1102 40-41 0.9559252170965422 1102 42-43 0.555593630166598 1102 44-45 1.1367239787971997 1102 46-47 1.4550294027171624 1102 48-49 1.3985931894546972 1102 50-51 1.2866972779341737 1102 52-53 1.617635838039412 1102 54-55 1.4665706061742938 1102 56-57 1.15351063465042 1102 58-59 1.317696517811747 1102 60-61 1.4039776194034346 1102 62-63 1.7005725128433085 1102 64-65 1.9798311367353243 1102 66-67 1.7510307326310368 1102 68-69 1.7167126052491923 1102 70-71 1.9269908172878623 1102 72-73 1.9143211406528202 1102 74-75 1.7056735160704442 1102 76-77 1.5920698172026597 1102 78-79 1.5861397488876392 1102 80-81 1.805713204445965 1102 82-83 1.7247851711639832 1102 84-85 2.1359593844554503 1102 86-87 1.7969564072156565 1102 88-89 2.078292665278333 1102 90-91 2.2582729524558403 1102 92-93 2.2866315683893923 1102 94-95 2.3868594133670733 1102 96-97 2.292497699637206 1102 98-99 2.7955886712207096 1102 100-101 2.860956021858062 1103 1 0.092868329944789 1103 2 0.2710086072465714 1103 3 0.28528678843546373 1103 4 0.39418945368090164 1103 5 0.01866657305077979 1103 6 0.1628982436624682 1103 7 0.20302392276629178 1103 8 0.24549311485768044 1103 9 0.19875702255337302 1103 10-11 0.30344731581497086 1103 12-13 0.22923106933973258 1103 14-15 0.26005660518728746 1103 16-17 0.45173360146991115 1103 18-19 0.4193093981749243 1103 20-21 0.3098460884068288 1103 22-23 0.36197719741187484 1103 24-25 0.4415592518836391 1103 26-27 0.24601432238045362 1103 28-29 0.3236553219466245 1103 30-31 0.35575816268261207 1103 32-33 0.24196854655570377 1103 34-35 0.14119523359925523 1103 36-37 0.2616120167155245 1103 38-39 -0.17317591238523988 1103 40-41 -0.023672368416534084 1103 42-43 0.10098598429134853 1103 44-45 0.16426925042697604 1103 46-47 0.063540469115555 1103 48-49 0.18326119750298986 1103 50-51 -0.1339465852449031 1103 52-53 -0.6423641619605895 1103 54-55 -0.19049176807117618 1103 56-57 -0.3105537516674879 1103 58-59 -0.45508014214801307 1103 60-61 -0.14153545906738785 1103 62-63 -0.27441742679451764 1103 64-65 -0.48322721336527863 1103 66-67 -0.02677610841524114 1103 68-69 0.22991180042021853 1103 70-71 0.42369101849510216 1103 72-73 0.2592104766689154 1103 74-75 0.5008847836760779 1103 76-77 0.36221066227308185 1103 78-79 0.5763610768554486 1103 80-81 0.6317896631984787 1103 82-83 0.9241010665362168 1103 84-85 0.7440881570912623 1103 86-87 0.42351576335248 1103 88-89 0.7220753614553956 1103 90-91 0.8614922683512098 1103 92-93 0.8643378058139355 1103 94-95 1.0370807413348793 1103 96-97 1.0214514219712072 1103 98-99 1.8029126148826826 1103 100-101 1.95580511642547 1104 1 0.1276733867004154 1104 2 0.2747518212475555 1104 3 0.28317601056066977 1104 4 0.27333603125958206 1104 5 0.013146661126405945 1104 6 0.1251699873320291 1104 7 0.2885332048740281 1104 8 0.05048153595027571 1104 9 0.1362233298309974 1104 10-11 -0.00818094431182459 1104 12-13 0.079369636592304 1104 14-15 0.17334007961878228 1104 16-17 0.42222067485360526 1104 18-19 0.07728745519630564 1104 20-21 0.15785109960610555 1104 22-23 0.01428728434859039 1104 24-25 -0.02981237409867532 1104 26-27 -0.03113536821596341 1104 28-29 -0.15388539404745671 1104 30-31 -0.6670010340069652 1104 32-33 -0.38431747143905426 1104 34-35 -0.19367056496571422 1104 36-37 -0.09683299296520431 1104 38-39 -0.5331660418412199 1104 40-41 -0.45749796349644356 1104 42-43 -0.6776139170032138 1104 44-45 -0.38815472740226653 1104 46-47 -0.1875851525389045 1104 48-49 -0.3096547889819554 1104 50-51 -0.622143692416234 1104 52-53 -0.6110973155994017 1104 54-55 -0.5341841108068337 1104 56-57 -0.44007427100995855 1104 58-59 -0.5138668245063123 1104 60-61 -0.5730035126720345 1104 62-63 -0.5878641448386297 1104 64-65 -0.34286428105442823 1104 66-67 -0.5845757363716579 1104 68-69 -0.5895407640230488 1104 70-71 -1.2895051395854544 1104 72-73 -1.4882934146032447 1104 74-75 -0.9914693410724134 1104 76-77 -1.3408681881881748 1104 78-79 -1.0747227848050827 1104 80-81 -1.1385455556618531 1104 82-83 -1.1159695458171477 1104 84-85 -1.129215817700885 1104 86-87 -1.0973832154258538 1104 88-89 -1.1721655557459272 1104 90-91 -1.0180073709942938 1104 92-93 -1.245282178241336 1104 94-95 -1.2935179451234937 1104 96-97 -0.8749686076404224 1104 98-99 -0.5167563422563823 1104 100-101 -0.5788822530745499 1105 1 -0.0972322736769442 1105 2 -0.1939813323912567 1105 3 0.03562884074934658 1105 4 0.026543578429389925 1105 5 -0.3625406704099845 1105 6 -0.31585427142807276 1105 7 -0.2163724555033255 1105 8 -0.30919501391495174 1105 9 -0.22410012030377402 1105 10-11 -0.4142186801608787 1105 12-13 -0.5844309024912562 1105 14-15 -0.4546114028610049 1105 16-17 -0.26438309873130095 1105 18-19 -0.2291815474991168 1105 20-21 -0.5257877144100718 1105 22-23 -0.4849040903144797 1105 24-25 -0.3990846112954358 1105 26-27 -0.6196798426094929 1105 28-29 -0.35485574445176127 1105 30-31 -0.4135778534139689 1105 32-33 -0.6196813528406793 1105 34-35 -0.5658268991975248 1105 36-37 -0.8709030738277406 1105 38-39 -0.23812561057437165 1105 40-41 -0.19550335433202548 1105 42-43 -0.07726351269054277 1105 44-45 -0.07184744977423208 1105 46-47 0.3921722598600219 1105 48-49 0.17859318945469838 1105 50-51 0.6281258493627426 1105 52-53 0.4704929808965552 1105 54-55 0.6437134633171553 1105 56-57 0.7406534917932746 1105 58-59 0.42055366066889377 1105 60-61 0.5454061908320078 1105 62-63 0.6548582271290222 1105 64-65 0.9612597081638974 1105 66-67 1.1096021612024636 1105 68-69 0.882426890963476 1105 70-71 0.6784193887164314 1105 72-73 0.585749712081391 1105 74-75 0.7642449446418738 1105 76-77 0.8477841029169468 1105 78-79 1.141854034601927 1105 80-81 1.305713204445965 1105 82-83 1.2933565997354144 1105 84-85 0.9716736701697357 1105 86-87 1.2940992643585183 1105 88-89 1.6568640938497623 1105 90-91 1.1611300953129806 1105 92-93 1.1852029969608218 1105 94-95 1.0154308419385032 1105 96-97 1.3853548424943476 1105 98-99 1.5155886712207085 1105 100-101 1.5409560218580651 1106 1 0.17547876528409745 1106 2 0.23564529098536724 1106 3 0.20793403555454404 1106 4 0.27087799401380863 1106 5 0.21169634257702796 1106 6 0.07784702727322212 1106 7 0.3761113107304368 1106 8 0.1867303107603675 1106 9 0.16921156800791692 1106 10-11 0.35739657957937965 1106 12-13 0.1847168247814679 1106 14-15 0.4339762594766654 1106 16-17 0.3014935246453234 1106 18-19 0.37361877717620473 1106 20-21 0.2868827401353826 1106 22-23 0.3084400655296733 1106 24-25 0.19060694714611515 1106 26-27 0.38352632622167704 1106 28-29 0.46658906074303985 1106 30-31 0.6291656530795322 1106 32-33 0.3960978679385434 1106 34-35 0.5228013475557205 1106 36-37 0.28965699110732857 1106 38-39 0.22671205176329323 1106 40-41 0.01784891839524505 1106 42-43 0.06566668211464588 1106 44-45 -0.4675049173066981 1106 46-47 -0.17846897390621308 1106 48-49 -0.5928191482076386 1106 50-51 -0.5520283714164762 1106 52-53 -0.3917959801424047 1106 54-55 -0.3359943288906386 1106 56-57 -0.3905153393755576 1106 58-59 -0.49717361205838273 1106 60-61 -0.5084168611160429 1106 62-63 -0.4685995650787689 1106 64-65 -0.4089594476802567 1106 66-67 -0.4366072543819506 1106 68-69 -0.1532468103352258 1106 70-71 -0.4601358060887648 1106 72-73 -0.5948428203861447 1106 74-75 -0.4425651202931924 1106 76-77 -0.5142938191609758 1106 78-79 -0.7379755108526211 1106 80-81 -0.5868923150345573 1106 82-83 -0.6172521664983535 1106 84-85 -0.6675795765835133 1106 86-87 -0.5340906707064192 1106 88-89 -0.3284768152411459 1106 90-91 -0.18673516442727944 1106 92-93 -0.36213466537684624 1106 94-95 0.29746006271772174 1106 96-97 -0.014288014648510483 1106 98-99 0.06388412576616176 1106 100-101 0.8503553725074156 1107 1 -0.03229280636459464 1107 2 0.013524720877509111 1107 3 0.02800979313030183 1107 4 0.2610391393656286 1107 5 -0.27987723215332494 1107 6 -0.13459519152493016 1107 7 -0.14544428762600603 1107 8 0.10360563176704574 1107 9 -0.29785314693815224 1107 10-11 -0.12688211841754082 1107 12-13 -0.1212105635082068 1107 14-15 -0.08719413086745931 1107 16-17 0.09543933871825772 1107 18-19 -0.12926225774931765 1107 20-21 -0.1525673754270187 1107 22-23 -0.4156546956413578 1107 24-25 0.06035848797817067 1107 26-27 -0.19946192493394932 1107 28-29 -0.10684121660673185 1107 30-31 -0.2805512190314019 1107 32-33 0.09813947040387916 1107 34-35 -0.016690498874673665 1107 36-37 -0.4993372949738273 1107 38-39 -0.2642676606147276 1107 40-41 0.05082432928379177 1107 42-43 -0.21674696708925723 1107 44-45 -0.29371992757891263 1107 46-47 -0.04774702988977708 1107 48-49 -0.07585394533142065 1107 50-51 -0.012632826989150914 1107 52-53 0.08334205272867834 1107 54-55 -0.07723084661020607 1107 56-57 -0.22359186736733605 1107 58-59 -0.18842939017856608 1107 60-61 -0.3285808955279599 1107 62-63 -0.39857195850455085 1107 64-65 -0.32160550571826363 1107 66-67 0.10527609179164799 1107 68-69 -0.20970385964184857 1107 70-71 -0.3626621286362699 1107 72-73 -0.2762196180235357 1107 74-75 -0.2317841110481993 1107 76-77 -0.14487933533971287 1107 78-79 -0.4595583947766073 1107 80-81 0.22691578717396865 1107 82-83 -0.21248682237919425 1107 84-85 -0.2558243120740116 1107 86-87 -0.06619936356723244 1107 88-89 -0.603531386376229 1107 90-91 -0.7750200257523936 1107 92-93 -0.43294873830955893 1107 94-95 -0.18075963425197372 1107 96-97 0.5524492734870812 1107 98-99 0.9576790667009369 1107 100-101 0.6520213971607269 1108 1 0.02069710031984684 1108 2 0.1478324717820989 1108 3 0.33325323882318614 1108 4 0.012546788702266554 1108 5 -0.08896121615637043 1108 6 8.551988768985552E-4 1108 7 0.1843659072575079 1108 8 0.19838123006578456 1108 9 0.2993188203061763 1108 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-1.5658611730358416 2316 86-87 -1.3575732095090807 2316 88-89 -1.0710627354185327 2316 90-91 -1.7918141555580966 2316 92-93 -2.065432891540926 2316 94-95 -1.8230360570162034 2316 96-97 -1.8709953317216836 2316 98-99 -2.1475210848768533 2316 100-101 -2.20445338580744 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 18.0 5 42.0 6 124.0 7 262.0 8 459.0 9 810.0 10 1462.0 11 2494.0 12 3676.0 13 4783.0 14 5579.0 15 7396.0 16 9967.0 17 13081.0 18 10240.0 19 4996.0 20 3881.0 21 3813.0 22 4187.0 23 4720.0 24 5303.0 25 5784.0 26 6766.0 27 7735.0 28 9149.0 29 10585.0 30 12209.0 31 14416.0 32 16961.0 33 20334.0 34 26516.0 35 34271.0 36 36787.0 37 37372.0 38 18389.0 39 1120.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.336907294063174 17.16456135781569 13.675681150612782 12.822850197508357 2 14.484083361297861 25.856871752739558 40.66900797278376 18.990036913178816 3 23.826550833311636 36.66021540838201 28.839438885176282 10.673794873130076 4 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19.21070330862412 24-25 17.72826942213788 26.60491541505635 40.10306982018467 15.563745342621091 26-27 19.896232310024416 23.599989585623533 41.80735717839827 14.696420925953783 28-29 16.159421652450497 24.59958718389699 44.374548889200675 14.866442274451833 30-31 15.474837063586424 29.93141194201691 41.4437048543914 13.150046140005264 32-33 15.142135606654497 28.92554333205088 39.77338143023683 16.158939631057795 34-35 16.31905017451944 26.15911139012363 39.98484074830001 17.53699768705692 36-37 18.78416515866231 26.42124890244767 40.7070045175037 14.087581421386322 38-39 20.82247262522963 23.957299697684174 41.31152994951759 13.908697727568601 40-41 16.248295054319605 27.132465781758274 42.6516298774464 13.967609286475714 42-43 15.093168869751613 30.410672004790424 39.3312495751245 15.164909550333464 44-45 15.894482275822567 31.16874851744068 35.86867851171294 17.06809069502381 46-47 16.2159269405336 29.78859733114255 33.09487374562832 20.90060198269553 48-49 18.58476839008473 29.601462586313133 33.035907998229625 18.777861025372516 50-51 20.983866908503938 31.151880169054664 32.341540179410856 15.522712743030546 52-53 17.100708883389334 31.73891221619714 34.272725563338824 16.887653337074703 54-55 14.659988023571 31.487738624562095 35.0230994651107 18.82917388675621 56-57 17.74563547738548 33.824580181379 30.23316024704572 18.196624094189797 58-59 16.857468913577065 31.418078940783566 31.543480493332886 20.18097165230648 60-61 17.17250905829856 29.658424398463882 33.20913278995596 19.9599337532816 62-63 22.173380371940574 29.036359454803453 31.653569047092127 17.13669112616385 64-65 20.04093618006133 30.453769600185154 33.56723369785338 15.938060521900132 66-67 16.68101035493758 31.315622373839425 34.26516682312135 17.738200448101644 68-69 16.38287901273139 34.491626673146634 32.45376517656452 16.67172913755746 70-71 16.65229930776329 33.89038187282863 29.662183857838357 19.795134961569723 72-73 16.58822406331721 32.01976927171322 29.007515447455507 22.384491217514057 74-75 17.364268201838446 32.75253310575762 30.75989556588968 19.12330312651426 76-77 18.757168872232434 30.498280917094934 32.40424267057396 18.340307540098674 78-79 16.17835095088493 30.377547891390254 34.66752295488865 18.776578202836166 80-81 15.148534614683651 35.176769064762766 31.91794652233154 17.756749798222042 82-83 15.193050109279104 33.983704322997504 29.889679452779998 20.933566114943396 84-85 16.292155146851428 30.825130723444875 29.816227787862786 23.066486341840907 86-87 17.99533381451262 30.70931515844925 31.191265872769065 20.104085154269068 88-89 19.768615890227075 27.625926129509143 32.50399960654865 20.101458373715136 90-91 16.071823787832543 29.469566890350542 34.27310455762524 20.185504764191677 92-93 14.390887723738926 32.47036702223829 32.37721930934732 20.761525944675466 94-95 14.311687748150398 33.136476390907845 30.212116609894917 22.33971925104684 96-97 14.596356840975558 30.705778893745535 28.718298817723447 25.979565447555466 98-99 16.59019902800278 29.61684216616524 29.423599861143252 24.36935894468873 100-101 18.492370000723223 30.35510233600926 29.589354162146524 21.563173501120993 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2912.0 1 2110.5 2 3172.5 3 4165.0 4 2634.0 5 2048.5 6 2677.5 7 4072.5 8 4960.0 9 4819.5 10 3931.5 11 3022.5 12 2776.5 13 2742.5 14 2680.0 15 2498.5 16 2292.5 17 2254.0 18 2346.5 19 2419.5 20 2472.0 21 2596.5 22 2763.5 23 2906.0 24 3140.0 25 3456.5 26 3768.0 27 4078.0 28 4369.5 29 4740.5 30 5070.0 31 5321.0 32 5607.5 33 5880.5 34 6277.0 35 6589.0 36 6848.5 37 7188.0 38 7102.0 39 6770.0 40 6487.0 41 6100.0 42 5813.0 43 5894.0 44 7847.5 45 8542.5 46 8740.5 47 13696.5 48 36089.5 49 35080.0 50 14254.5 51 9808.0 52 4983.5 53 3160.0 54 2601.0 55 2284.0 56 2160.0 57 2102.5 58 2039.0 59 2146.0 60 2304.5 61 2279.0 62 1850.5 63 990.5 64 412.5 65 308.0 66 246.0 67 192.5 68 157.5 69 136.5 70 136.0 71 126.0 72 109.0 73 130.0 74 119.5 75 77.0 76 62.5 77 50.0 78 40.5 79 30.5 80 27.5 81 20.5 82 14.0 83 10.0 84 4.0 85 2.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03934149283608772 2 0.004339135239274381 3 0.006364065017602425 4 0.0 5 0.0011571027304731682 6 5.785513652365841E-4 7 0.0 8 0.0 9 0.0011571027304731682 10-11 4.3391352392743813E-4 12-13 0.0013017405717823142 14-15 0.0026034811435646284 16-17 0.0023142054609463364 18-19 0.0 20-21 0.0 22-23 0.0010124648891640222 24-25 8.678270478548763E-4 26-27 0.004339135239274381 28-29 0.004773048763201819 30-31 0.0011571027304731682 32-33 0.0 34-35 0.007955081272003032 36-37 0.010269286732949369 38-39 0.007521167748075594 40-41 0.0015910162544006063 42-43 4.3391352392743813E-4 44-45 0.0014463784130914602 46-47 0.0 48-49 5.785513652365841E-4 50-51 0.0011571027304731682 52-53 0.0018802919370188985 54-55 0.003471308191419505 56-57 0.0017356540957097525 58-59 4.3391352392743813E-4 60-61 0.0039052217153469427 62-63 0.005062324445820111 64-65 0.008967546161167055 66-67 0.0030373946674920665 68-69 0.0028927568261829205 70-71 0.0 72-73 8.678270478548763E-4 74-75 0.005351600128438403 76-77 0.0036159460327286506 78-79 0.003760583874037797 80-81 0.004049859556656089 82-83 0.004773048763201819 84-85 0.005351600128438403 86-87 0.0036159460327286506 88-89 0.009256821843785346 90-91 0.011426389463422535 92-93 0.0010124648891640222 94-95 0.0024588433022554824 96-97 5.785513652365841E-4 98-99 0.0031820325088012125 100-101 0.004628410921892673 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 345691.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.69292463596879 #Duplication Level Percentage of deduplicated Percentage of total 1 84.65318585209899 34.44785712074123 2 8.75714083232667 7.127073438328684 3 2.6453158890790145 3.229369203378693 4 1.1604952387850032 1.8889578116911494 5 0.602443655488346 1.2257597135102403 6 0.3744864416342589 0.9143369127969014 7 0.27031185908747946 0.7699846077038792 8 0.21405569046458367 0.6968441663980453 9 0.14861639241736654 0.5442872090678513 >10 0.9407233382037165 7.325711932012702 >50 0.1023913801819861 2.9189380823812705 >100 0.09670297017187575 8.199820213779027 >500 0.012798922522748262 3.3966508871008863 >1k 0.017065230030331016 14.111480003740539 >5k 0.003555256256318962 10.309163851820212 >10k+ 7.110512512637923E-4 2.8937648455486804 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 10001 2.8930461018655387 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 8539 2.470125053877596 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8156 2.3593324674347897 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7043 2.037368632680631 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6561 1.8979377536586142 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5330 1.5418393883554966 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4975 1.439146521026003 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4699 1.3593064326233544 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3096 0.8955975133862322 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3056 0.8840264860815005 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2745 0.7940617487872117 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2426 0.7017828060319765 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2415 0.6986007735231753 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2399 0.6939723626012827 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2109 0.610082414641978 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1894 0.5478881428790452 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1778 0.5143321636953233 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1696 0.4906115577206233 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1625 0.4700729842547246 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1556 0.4501129621540624 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1531 0.44288107008860517 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1342 0.38820796607374797 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1313 0.37981897127781744 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1255 0.36304098168595655 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1205 0.34857719755504196 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1178 0.3407667541243481 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1152 0.33324558637627244 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1120 0.3239887645324871 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1119 0.3236994888498688 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1086 0.31415339132346515 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 910 0.2632408711826458 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 901 0.26063739003908115 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 856 0.247619984321258 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 765 0.22129589720299342 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 743 0.21493183218539103 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 691 0.1998894966892398 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 671 0.19410398303687398 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 650 0.18802919370188984 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 604 0.17472251230144842 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 587 0.16980482569693744 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 566 0.1637300363619533 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 564 0.16315148499671672 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 561 0.16228365794886185 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 556 0.16083727953577037 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 550 0.15910162544006062 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 536 0.15505176588340455 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 519 0.15013407927889358 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 509 0.14724132245271065 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 500 0.14463784130914603 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 489 0.1414558088003448 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 482 0.13943087902201676 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 464 0.1342239167348875 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 464 0.1342239167348875 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 462 0.13364536536965094 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 450 0.1301740571782314 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 434 0.12554564625633877 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 427 0.12352071647801072 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 418 0.12091723533444608 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 416 0.1203386839692095 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 408 0.11802447850826316 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 401 0.1159995487299351 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 383 0.11079258644280585 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 361 0.10442852142520344 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 355 0.10269286732949368 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 350 0.10124648891640221 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 347 0.10037866186854735 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.8927568261829205E-4 0.0 12-13 0.0 0.0 0.0 5.785513652365841E-4 0.0 14-15 0.0 0.0 0.0 5.785513652365841E-4 0.0 16-17 0.0 0.0 0.0 5.785513652365841E-4 0.0 18-19 0.0 0.0 0.0 8.678270478548761E-4 0.0 20-21 0.0 0.0 0.0 8.678270478548761E-4 0.0 22-23 0.0 0.0 0.0 0.0014463784130914602 0.0 24-25 0.0 0.0 0.0 0.0027481189848737745 0.0 26-27 0.0 0.0 0.0 0.004483773080583527 0.0 28-29 0.0 0.0 0.0 0.009112184002476199 0.0 30-31 0.0 0.0 0.0 0.01706726527447923 0.0 32-33 0.0 0.0 0.0 0.026324087118264575 0.0 34-35 0.0 0.0 0.0 0.03630409816859566 0.0 36-37 0.0 0.0 0.0 0.05033396877558282 0.0 38-39 0.0 0.0 0.0 0.05973542846067731 0.0 40-41 0.0 0.0 0.0 0.07318674770242789 0.0 42-43 0.0 0.0 0.0 0.0964734401532004 0.0 44-45 0.0 0.0 0.0 0.13205434911525032 0.0 46-47 0.0 0.0 0.0 0.20451790761113248 0.0 48-49 0.0 0.0 0.0 0.3333902242175816 0.0 50-51 0.0 0.0 0.0 0.4172801721768863 0.0 52-53 0.0 0.0 0.0 0.49596315784906175 0.0 54-55 0.0 0.0 0.0 0.5908455817478615 0.0 56-57 0.0 0.0 0.0 0.6640323294502894 0.0 58-59 0.0 0.0 0.0 0.7633985264296728 0.0 60-61 0.0 0.0 0.0 0.9504152552423986 0.0 62-63 0.0 0.0 0.0 1.0711878527355356 0.0 64-65 0.0 0.0 0.0 1.1782198553043037 0.0 66-67 0.0 0.0 0.0 1.370588184245468 2.8927568261829205E-4 68-69 0.0 0.0 0.0 1.5928965463376252 2.8927568261829205E-4 70-71 0.0 0.0 0.0 1.7705118154652566 2.8927568261829205E-4 72-73 0.0 0.0 0.0 1.9942665559705055 2.8927568261829205E-4 74-75 0.0 0.0 0.0 2.1636374681435155 2.8927568261829205E-4 76-77 0.0 0.0 0.0 2.276021070840722 2.8927568261829205E-4 78-79 0.0 0.0 0.0 2.429047906945798 2.8927568261829205E-4 80-81 0.0 0.0 0.0 2.678982096728003 2.8927568261829205E-4 82-83 0.0 0.0 0.0 2.92342004854046 2.8927568261829205E-4 84-85 0.0 0.0 0.0 3.3100369983598066 2.8927568261829205E-4 86-87 0.0 0.0 0.0 3.998223847308724 2.8927568261829205E-4 88-89 0.0 0.0 0.0 4.582416088356364 2.8927568261829205E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 25 5.268999E-5 76.003235 2 ATAACGC 30 1.2987394E-4 63.345192 3 TCACGCA 50 4.231988E-7 57.00243 4 TTCAACG 55 8.171537E-7 51.82039 2 ATCACGC 60 1.4873112E-6 47.508896 3 TACCTGG 285 0.0 45.00192 2 GTATACT 45 9.613701E-4 42.224026 4 GTTCAAC 70 4.283096E-6 40.739597 1 ACCTGGG 320 0.0 40.085636 3 TATCACG 75 6.90623E-6 38.00162 2 TGTAGTG 50 0.0016139144 38.001617 5 AAGTACT 245 0.0 34.89945 4 AAGTGTA 55 0.002576602 34.546925 7 AGTACTC 235 0.0 34.36317 5 GGAGTTC 70 2.0498595E-4 33.930016 8 ATCAAGC 60 0.00393517 31.686356 1 GTATATG 105 1.887407E-6 31.686354 1 TGGGAGA 555 0.0 31.668018 6 TCAAGCA 60 0.0039463397 31.668016 2 ACACGAC 30 0.003951846 31.663437 94-95 >>END_MODULE