##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936632_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 354044 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.640671780908587 31.0 31.0 34.0 30.0 34.0 2 31.619671566246005 31.0 31.0 34.0 30.0 34.0 3 31.65137948955497 31.0 31.0 34.0 30.0 34.0 4 35.44209476788196 37.0 35.0 37.0 33.0 37.0 5 35.31318141247981 37.0 35.0 37.0 33.0 37.0 6 35.525236976195046 37.0 35.0 37.0 33.0 37.0 7 35.4801154658743 37.0 35.0 37.0 33.0 37.0 8 35.53698410367073 37.0 35.0 37.0 33.0 37.0 9 37.01432590299511 39.0 37.0 39.0 33.0 39.0 10-11 36.87708166216628 39.0 37.0 39.0 32.5 39.0 12-13 36.72897718927591 39.0 36.0 39.0 32.0 39.0 14-15 37.39530538577126 39.0 36.0 41.0 32.0 41.0 16-17 37.42117646394233 39.0 36.5 40.0 32.0 41.0 18-19 37.898372801120765 39.5 37.0 41.0 33.0 41.0 20-21 37.99257436928743 40.0 37.0 41.0 33.0 41.0 22-23 37.822024946051904 40.0 36.0 41.0 33.0 41.0 24-25 37.5944967292201 39.0 36.0 41.0 32.5 41.0 26-27 37.09118216944786 39.0 35.0 41.0 31.5 41.0 28-29 36.79542796940493 38.5 35.0 40.5 31.0 41.0 30-31 36.389112652664636 38.0 35.0 40.0 31.0 41.0 32-33 35.799714159821946 37.5 35.0 40.0 30.0 41.0 34-35 35.38275044909672 37.0 35.0 40.0 30.0 41.0 36-37 34.86844996667081 37.0 34.0 40.0 27.0 41.0 38-39 34.408202087876084 36.5 33.5 40.0 25.5 41.0 40-41 33.93515212798409 36.0 33.0 40.0 23.5 41.0 42-43 33.431857339765685 36.0 33.0 40.0 20.5 41.0 44-45 32.68402938617799 36.0 33.0 40.0 10.0 41.0 46-47 31.53986933827434 35.0 31.5 40.0 2.0 41.0 48-49 30.558117070194665 35.0 30.0 40.0 2.0 41.0 50-51 30.06588587859136 35.0 28.5 40.0 2.0 41.0 52-53 29.487058105772164 35.0 27.0 39.0 2.0 41.0 54-55 29.315426896092013 35.0 27.0 39.0 2.0 40.0 56-57 29.132863429404253 35.0 26.5 39.0 2.0 40.0 58-59 28.87452407045452 35.0 26.0 39.0 2.0 40.0 60-61 28.39304154285908 35.0 25.5 38.5 2.0 40.0 62-63 27.89020432488617 34.5 23.5 38.0 2.0 40.0 64-65 27.261934957236953 34.0 22.0 37.0 2.0 39.5 66-67 26.84040542983358 33.0 21.5 36.5 2.0 39.0 68-69 26.64423772186508 33.0 21.0 36.0 2.0 39.0 70-71 26.30437036074612 33.0 19.5 36.0 2.0 38.5 72-73 25.774160838765802 33.0 16.5 35.0 2.0 37.5 74-75 25.24182587475003 33.0 10.5 35.0 2.0 37.0 76-77 23.693769700941125 30.5 7.0 33.5 2.0 35.5 78-79 24.26754725401363 31.5 2.0 35.0 2.0 36.0 80-81 24.11013602829027 32.0 2.0 35.0 2.0 36.0 82-83 23.824689869055824 32.0 2.0 35.0 2.0 35.0 84-85 23.46002474268735 32.0 2.0 35.0 2.0 35.0 86-87 22.83884488933579 31.0 2.0 34.0 2.0 35.0 88-89 22.113638417823772 30.5 2.0 34.0 2.0 35.0 90-91 21.43154523166612 29.0 2.0 34.0 2.0 35.0 92-93 20.926986476257188 29.0 2.0 34.0 2.0 35.0 94-95 20.488108822632213 29.0 2.0 34.0 2.0 35.0 96-97 19.62650828710556 27.5 2.0 34.0 2.0 35.0 98-99 18.736538396357517 26.0 2.0 34.0 2.0 35.0 100-101 16.268283038266432 19.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27250034740385587 1101 2 0.2863094935819426 1101 3 0.4487397258604098 1101 4 0.44613276309068084 1101 5 0.3996835972247439 1101 6 0.13326585154744208 1101 7 0.3204684015650514 1101 8 0.20010654783179405 1101 9 0.22697814841188801 1101 10-11 0.4572570974631205 1101 12-13 0.5214792882198438 1101 14-15 0.809999646287217 1101 16-17 0.7554643734535063 1101 18-19 0.7993518684975811 1101 20-21 0.42023059624469283 1101 22-23 0.4944952978122146 1101 24-25 0.5129212568153037 1101 26-27 0.5573821753235748 1101 28-29 0.5193275096985914 1101 30-31 0.46273447818825986 1101 32-33 0.4772451978944261 1101 34-35 0.15402518013036826 1101 36-37 0.544137282229677 1101 38-39 0.24243942811371966 1101 40-41 0.5272265910927416 1101 42-43 0.4166261476980324 1101 44-45 0.12754092156944807 1101 46-47 -0.15301173444591143 1101 48-49 -0.1246782696902855 1101 50-51 -0.025075012013935805 1101 52-53 0.2891785294611111 1101 54-55 -0.05246873155433818 1101 56-57 0.015337867733929755 1101 58-59 -0.1900322026646144 1101 60-61 -0.02466054253177674 1101 62-63 0.27998214637977625 1101 64-65 0.293414535069509 1101 66-67 0.03086491454912732 1101 68-69 0.3409436293232666 1101 70-71 0.2057167213947153 1101 72-73 0.27046068746924945 1101 74-75 -0.1854334212380273 1101 76-77 -0.5585801770820176 1101 78-79 -0.5768307743896024 1101 80-81 -0.22182525671751918 1101 82-83 0.12842403148554382 1101 84-85 0.286185924241007 1101 86-87 0.150454023329317 1101 88-89 0.3172645616937473 1101 90-91 0.12164909578566707 1101 92-93 0.23955157896958212 1101 94-95 0.5552383356752593 1101 96-97 1.1872963268136978 1101 98-99 1.2165726859359367 1101 100-101 1.714930364501317 1102 1 0.17483570014960748 1102 2 0.08273155077691641 1102 3 0.1676179748344211 1102 4 0.013286368718979702 1102 5 0.19696064757977183 1102 6 0.08090769898409178 1102 7 0.2155159554703303 1102 8 0.19272103548731678 1102 9 0.3615785001805065 1102 10-11 0.24744584738169095 1102 12-13 0.2213766890406319 1102 14-15 0.4849271754384077 1102 16-17 0.4974885086889884 1102 18-19 0.09644570603259695 1102 20-21 0.17830824590920713 1102 22-23 0.15937462163476823 1102 24-25 0.0023193416306241943 1102 26-27 0.24296047641144725 1102 28-29 -0.028890033132491055 1102 30-31 -0.03987528664790574 1102 32-33 0.3560153105906707 1102 34-35 0.9833644739874501 1102 36-37 1.1554981056288085 1102 38-39 1.0732769956988903 1102 40-41 0.9513316982518845 1102 42-43 1.2557001493917355 1102 44-45 1.3408047154591003 1102 46-47 1.3751623258758876 1102 48-49 1.2434329282772723 1102 50-51 1.3210465761562133 1102 52-53 1.2348864637976398 1102 54-55 1.412794117771444 1102 56-57 1.023838942582472 1102 58-59 1.1998691066963367 1102 60-61 1.257308058862268 1102 62-63 1.3660677434092854 1102 64-65 1.3992179029282816 1102 66-67 1.4898578140345045 1102 68-69 1.4754503389838725 1102 70-71 1.2091244798469312 1102 72-73 1.381919224372382 1102 74-75 1.73425422126083 1102 76-77 1.5087159274062891 1102 78-79 1.5685506339877016 1102 80-81 1.3137994582847625 1102 82-83 0.9855953884003981 1102 84-85 0.9324386113623682 1102 86-87 1.1852033881915425 1102 88-89 0.9316382181330667 1102 90-91 1.4203348491567844 1102 92-93 1.1571750236963076 1102 94-95 1.0496075950069006 1102 96-97 1.433489571553455 1102 98-99 1.1010712528045445 1102 100-101 1.6321914549840209 1103 1 0.2094364256857233 1103 2 0.3689279965683454 1103 3 -0.0220316555095188 1103 4 0.18518791622322794 1103 5 -0.04255811074322935 1103 6 0.11766666486553135 1103 7 0.15458010572059777 1103 8 0.18560343434850068 1103 9 -0.28665128353625846 1103 10-11 0.21832108272172945 1103 12-13 0.053424262168242365 1103 14-15 -0.15944532766118868 1103 16-17 0.008061590380684436 1103 18-19 0.10365514718610314 1103 20-21 -0.2726147462384745 1103 22-23 -0.09431045895484402 1103 24-25 0.03840098740369058 1103 26-27 -0.044544689589379516 1103 28-29 -0.4414124191360713 1103 30-31 0.06151608957471666 1103 32-33 0.02261309724632099 1103 34-35 0.1675037034497251 1103 36-37 0.174681188698095 1103 38-39 0.01049286565343266 1103 40-41 -0.20271844639613334 1103 42-43 0.14236957125883976 1103 44-45 0.5636430311132301 1103 46-47 0.4420448165770914 1103 48-49 0.22970284608046043 1103 50-51 -0.10631945222235117 1103 52-53 -0.16635108040036783 1103 54-55 -0.35781248552561706 1103 56-57 -0.41041508689817263 1103 58-59 -0.41790423214739647 1103 60-61 0.0458693850320806 1103 62-63 -0.2878790812222114 1103 64-65 -0.23412231840615405 1103 66-67 -0.04617791173121333 1103 68-69 0.19349001387022113 1103 70-71 -0.2054298664817651 1103 72-73 -0.11081393446492527 1103 74-75 -0.0077901374662658895 1103 76-77 -0.1574570991813964 1103 78-79 0.16703756974560946 1103 80-81 -0.1501992559550338 1103 82-83 -0.28998205569198987 1103 84-85 -0.11699427654689387 1103 86-87 -0.5917860961267749 1103 88-89 -0.646104354305745 1103 90-91 -0.48892721632514835 1103 92-93 -0.3348463750271158 1103 94-95 0.18812529716973003 1103 96-97 0.3809915231537637 1103 98-99 0.3713334560464965 1103 100-101 0.48934579212871654 1104 1 0.1853227287485133 1104 2 -0.05063841846418882 1104 3 0.1233801162032293 1104 4 0.22707978799405026 1104 5 0.16376698864480943 1104 6 0.17583286250999208 1104 7 0.1501132276217021 1104 8 0.3280372873286552 1104 9 0.583340229395354 1104 10-11 0.3297659688907828 1104 12-13 0.22570430564586275 1104 14-15 0.5647323945287752 1104 16-17 0.20856858104490783 1104 18-19 0.0600807513313697 1104 20-21 0.32351619284819577 1104 22-23 0.21160368764810045 1104 24-25 0.24242192217237601 1104 26-27 0.08060234514804421 1104 28-29 0.1270797117136695 1104 30-31 0.42533586593301465 1104 32-33 0.008073409034409451 1104 34-35 -0.23872163566077376 1104 36-37 -0.23832263792747455 1104 38-39 -0.18021677238054679 1104 40-41 0.06900547075816377 1104 42-43 0.007201839986230141 1104 44-45 -0.11284704497258957 1104 46-47 -0.28006325209371496 1104 48-49 -0.5981511167848943 1104 50-51 -0.4372518385775237 1104 52-53 -0.37699043955225875 1104 54-55 -0.41082688376268806 1104 56-57 -0.6535360946258741 1104 58-59 -0.5771670483651441 1104 60-61 -0.5316309499837786 1104 62-63 -0.3917116626310637 1104 64-65 -0.256103081051144 1104 66-67 -0.37674550304164356 1104 68-69 -0.1834884408877464 1104 70-71 -0.38693029083518127 1104 72-73 -0.09367973455723799 1104 74-75 -0.19925542237864136 1104 76-77 -0.010567535297592912 1104 78-79 0.21068269117024485 1104 80-81 0.426050353193137 1104 82-83 0.26439846276366197 1104 84-85 0.3711536068973693 1104 86-87 0.201769687735883 1104 88-89 0.12372486205494226 1104 90-91 0.5316278677266091 1104 92-93 0.3148200980748328 1104 94-95 0.9805718063044999 1104 96-97 1.198508385618588 1104 98-99 1.053738423214945 1104 100-101 1.472241371408355 1105 1 0.12187181189160157 1105 2 0.1907563823311591 1105 3 0.17111559009674338 1105 4 0.34058593719502284 1105 5 0.17875336466660485 1105 6 0.15655309227654612 1105 7 -0.09287226468259036 1105 8 0.15071936995624213 1105 9 0.37050423263564625 1105 10-11 0.24021592838706596 1105 12-13 0.4112812995334778 1105 14-15 0.597562499838439 1105 16-17 0.1657487857727915 1105 18-19 0.33077078741649757 1105 20-21 0.3506831418135121 1105 22-23 0.6275326956751925 1105 24-25 0.489759723188655 1105 26-27 0.5303630049904484 1105 28-29 0.4695239270461755 1105 30-31 0.7158064455044197 1105 32-33 0.7176286045569071 1105 34-35 0.5760474931410542 1105 36-37 0.8347092495458313 1105 38-39 0.7673530044807819 1105 40-41 1.025364554637683 1105 42-43 0.6966230050204771 1105 44-45 0.8944878103186653 1105 46-47 1.0378430738507554 1105 48-49 0.8991903663876499 1105 50-51 0.4928461067792753 1105 52-53 0.5546797865321373 1105 54-55 1.0588763344418943 1105 56-57 0.5074183202794948 1105 58-59 0.20426383756165833 1105 60-61 0.4018950828610599 1105 62-63 -0.49308510692003793 1105 64-65 0.009748601694273162 1105 66-67 0.33146202328577345 1105 68-69 0.40185657715482037 1105 70-71 0.4176981795971031 1105 72-73 0.5963563505742151 1105 74-75 0.21769354293544652 1105 76-77 0.35101284617379847 1105 78-79 0.06587821366822055 1105 80-81 -0.004430536415821251 1105 82-83 0.2636141634779996 1105 84-85 0.22381948803736051 1105 86-87 0.5039973922796648 1105 88-89 0.26500183384962384 1105 90-91 0.6696534955342521 1105 92-93 0.4724789831179166 1105 94-95 0.7186968523314512 1105 96-97 0.3596800477439288 1105 98-99 -0.36847602556626313 1105 100-101 -0.2393279635942207 1106 1 -0.1495251787666021 1106 2 -0.14228932828711294 1106 3 -0.2812412522337162 1106 4 -0.007777465785800075 1106 5 0.07717025121014132 1106 6 -0.20288497375782555 1106 7 -0.34492246792013503 1106 8 -0.12692450082747087 1106 9 -0.24778714581404415 1106 10-11 -0.40911017872268474 1106 12-13 0.02954058784553837 1106 14-15 -0.3123426559769982 1106 16-17 -0.1921525192261342 1106 18-19 -0.13484997846811808 1106 20-21 -0.26960495631099946 1106 22-23 -0.22883184828829428 1106 24-25 -0.0925122585556224 1106 26-27 -0.03774652934600198 1106 28-29 -0.3668056743230039 1106 30-31 -0.3981563299359365 1106 32-33 -0.49551052401757545 1106 34-35 -0.5332907369095707 1106 36-37 -0.7473274045224869 1106 38-39 -0.29392846168194353 1106 40-41 -0.6172440169946327 1106 42-43 -0.16603477148954227 1106 44-45 -0.7359193778721611 1106 46-47 -1.125706095371239 1106 48-49 -1.2681632669290437 1106 50-51 -0.5769749015641601 1106 52-53 -0.926141007877046 1106 54-55 -0.6914053456550882 1106 56-57 -1.3165637092434288 1106 58-59 -1.2459857523976972 1106 60-61 -0.9877825895342909 1106 62-63 -0.7268902841289098 1106 64-65 -0.8643867473751143 1106 66-67 -0.801159384401597 1106 68-69 -0.979502618452397 1106 70-71 -0.9506502479861538 1106 72-73 -0.6972327530412734 1106 74-75 -0.7452066923922267 1106 76-77 -0.7463486313987637 1106 78-79 -0.7109812315289545 1106 80-81 -0.7167246246993564 1106 82-83 -0.4972394341829869 1106 84-85 -0.2424466461707233 1106 86-87 -0.2763384844942074 1106 88-89 -0.182106488192737 1106 90-91 0.10492055679076273 1106 92-93 -0.06951987589941311 1106 94-95 -0.42331983454011635 1106 96-97 0.025775034551319465 1106 98-99 0.15432580000600993 1106 100-101 -0.038636242849729996 1107 1 0.14986509014394755 1107 2 0.0486754412864876 1107 3 0.10574659415779664 1107 4 0.19421988202067553 1107 5 -0.08031399284634944 1107 6 0.14341767706859088 1107 7 0.04632392632706939 1107 8 0.018412970292331465 1107 9 0.07018214889387764 1107 10-11 0.33232487671362776 1107 12-13 0.14548519254566372 1107 14-15 0.22647301657279684 1107 16-17 0.32728778591283003 1107 18-19 0.11314720528514499 1107 20-21 0.013916582508095132 1107 22-23 -0.2681191738108737 1107 24-25 0.12392860709847042 1107 26-27 0.2769041056766284 1107 28-29 0.041054525001641196 1107 30-31 -0.29356899210936405 1107 32-33 -0.3950586941407579 1107 34-35 -0.4586465273042606 1107 36-37 -0.34720504817158115 1107 38-39 -0.9242448109512225 1107 40-41 -1.1095745295241954 1107 42-43 -1.1610789975001836 1107 44-45 -1.4556242188089072 1107 46-47 -1.4589220850905669 1107 48-49 -1.1276756187684889 1107 50-51 -0.9121321875772566 1107 52-53 -0.3414622103870606 1107 54-55 -0.8503660685828933 1107 56-57 -0.45188709912955005 1107 58-59 -0.9917101156661836 1107 60-61 -0.7568571927305996 1107 62-63 -0.7610167691522172 1107 64-65 -0.7816321573030649 1107 66-67 -0.7479036117793463 1107 68-69 -0.7962039185740686 1107 70-71 -0.36012705496388264 1107 72-73 -0.8329252626452224 1107 74-75 -0.6667414101523548 1107 76-77 -0.5132358584894128 1107 78-79 -0.7815241109361786 1107 80-81 -0.5992351856246145 1107 82-83 -0.6056478446441105 1107 84-85 -0.5690526587207962 1107 86-87 -0.25189113873840085 1107 88-89 -0.27681303801706036 1107 90-91 -0.4283719910922663 1107 92-93 -0.2932611093061155 1107 94-95 0.2867195382297858 1107 96-97 -0.08643394189337883 1107 98-99 -0.19381561385922197 1107 100-101 0.01901820640998153 1108 1 -0.024486535556665245 1108 2 -0.08606076178843836 1108 3 -0.026483644014753338 1108 4 0.05269286701312126 1108 5 0.16921657992546102 1108 6 0.13223613226476516 1108 7 0.05747017585058245 1108 8 0.033759358173341525 1108 9 0.19914404410924647 1108 10-11 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-2.283389456279565 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 13.0 5 34.0 6 125.0 7 277.0 8 459.0 9 871.0 10 1438.0 11 2534.0 12 3747.0 13 4942.0 14 5769.0 15 7703.0 16 10297.0 17 13313.0 18 10490.0 19 5229.0 20 4015.0 21 3810.0 22 4242.0 23 4799.0 24 5354.0 25 5971.0 26 6863.0 27 7881.0 28 9207.0 29 10980.0 30 12460.0 31 14570.0 32 17104.0 33 20885.0 34 27461.0 35 35549.0 36 37648.0 37 38219.0 38 18615.0 39 1165.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.56623868243005 16.993940421204286 13.550009181179993 12.889811715185672 2 14.626670885074745 25.77936972463588 40.647280821685634 18.946678568603744 3 23.81140699017827 36.762699464140944 28.750236574569715 10.675656971111067 4 10.390798883754561 17.990419269921254 36.945125464631516 34.673656381692666 5 9.293175231757402 45.34423247484705 30.103605868362926 15.258986425032624 6 21.092858514760877 36.858977980138064 21.616522240173538 20.43164126492752 7 22.568946232671646 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14.825433186407503 30-31 15.397836402666364 29.72630211275562 41.750084736188 13.125776748390011 32-33 15.10277121417343 28.734416245662253 40.08903590107185 16.073776639092465 34-35 16.222361567750173 26.11386977595499 40.2206272808415 17.443141375453344 36-37 18.606487110937874 26.2969914458939 41.085694998171114 14.010826444997113 38-39 20.68484367453119 23.872222394834438 41.61387086660434 13.82906306403003 40-41 16.191841374976168 27.05197822295346 42.82932133853986 13.92685906353051 42-43 15.039114635045415 30.339511360121573 39.54003053469614 15.081343470136868 44-45 15.922042156551214 30.94107262648731 36.22370511598348 16.913180100978003 46-47 16.129159817196832 29.748447924257576 33.273312205896474 20.84908005264912 48-49 18.525255549498503 29.55688747913377 33.229343494115085 18.688513477252634 50-51 20.940758775745387 31.02820553377546 32.54200042932517 15.48903526115398 52-53 17.209866570256803 31.590423789133553 34.35773519675521 16.841974443854436 54-55 14.636867563464323 31.39286092574939 35.278042580235144 18.692228930551142 56-57 17.71071982462732 33.784524377876146 30.386819929432836 18.117935868063697 58-59 16.84223165537749 31.340363466849364 31.725302123872552 20.092102753900594 60-61 17.141396177962477 29.617330110869883 33.32029092573314 19.9209827854345 62-63 22.072094340448793 29.104265744241793 31.719278124377638 17.10436179093178 64-65 20.079410817499195 30.371742065980754 33.625438581622795 15.923408534897254 66-67 16.787724290849006 31.14586937639906 34.38243664070389 17.683969692048045 68-69 16.508703174098788 34.39663877414116 32.405183066765524 16.689474984994526 70-71 16.55742867754951 33.85177245169026 29.83574052341026 19.755058347349973 72-73 16.52548835737931 31.963682480143714 29.28802634700772 22.222802815469265 74-75 17.412820092758405 32.65553415170123 30.92690615131709 19.004739604223275 76-77 18.70432170512529 30.39909501367789 32.534738453586264 18.361844827610557 78-79 16.204367396920137 30.333931716521732 34.716158747152264 18.745542139405863 80-81 15.196744513216586 34.980521883571996 32.09807139810219 17.724662205109226 82-83 15.225970665340807 33.89672979569698 30.051075754836376 20.826223784125837 84-85 16.285554857074242 30.917168749161235 29.951898966640062 22.845377427124465 86-87 17.934543112507203 30.777053366628625 31.16419588479226 20.124207636071908 88-89 19.709807079633514 27.61692839129421 32.63087997400414 20.042384555068132 90-91 16.139323670951537 29.422885094141815 34.30616506678798 20.13162616811866 92-93 14.385449408440756 32.39992936606039 32.550662193183825 20.663959032315027 94-95 14.392230266643121 32.978280063570544 30.462299134734238 22.167190535052093 96-97 14.534610993681401 30.747210887429492 28.86392408640315 25.854254032485958 98-99 16.561893740612472 29.58482226753198 29.693904801468374 24.159379190387174 100-101 18.49108914248894 30.1820324555305 29.95777728791142 21.36910111406915 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3134.0 1 2241.5 2 3465.0 3 4593.0 4 2807.0 5 2072.0 6 2734.5 7 4201.5 8 5085.0 9 4970.0 10 4122.0 11 3130.0 12 2852.5 13 2837.5 14 2746.5 15 2556.0 16 2400.5 17 2308.5 18 2310.5 19 2398.0 20 2509.0 21 2676.5 22 2870.0 23 3036.5 24 3213.0 25 3459.0 26 3790.0 27 4136.0 28 4458.0 29 4838.0 30 5155.0 31 5422.0 32 5734.5 33 6019.0 34 6421.5 35 6727.5 36 6973.5 37 7303.5 38 7204.5 39 6857.5 40 6543.5 41 6278.0 42 6026.5 43 6008.0 44 8067.0 45 8842.0 46 8951.5 47 14179.0 48 36772.0 49 35607.5 50 14528.0 51 9977.0 52 5181.5 53 3266.5 54 2653.0 55 2360.5 56 2230.0 57 2094.0 58 2084.5 59 2243.0 60 2375.5 61 2283.5 62 1843.5 63 1035.0 64 447.5 65 297.5 66 245.5 67 195.5 68 148.5 69 139.0 70 130.5 71 108.5 72 105.0 73 138.5 74 128.5 75 74.5 76 50.0 77 39.0 78 41.5 79 41.5 80 28.5 81 18.5 82 13.5 83 9.5 84 6.0 85 2.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016664595361028572 2 0.01129803075323971 3 0.00932087537142276 4 0.0 5 0.0028245076883099277 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.473523064929782E-4 14-15 5.649015376619855E-4 16-17 0.0 18-19 0.001129803075323971 20-21 0.0 22-23 1.4122538441549637E-4 24-25 0.0 26-27 0.0016947046129859565 28-29 0.0029657330727254237 30-31 0.001129803075323971 32-33 0.008332297680514286 34-35 0.002259606150647942 36-37 9.885776909084748E-4 38-39 0.0019771553818169496 40-41 4.236761532464891E-4 42-43 0.005790240761035351 44-45 0.001835929997401453 46-47 5.649015376619855E-4 48-49 8.473523064929782E-4 50-51 0.0 52-53 0.0 54-55 0.001835929997401453 56-57 0.015534792285704601 58-59 0.025844245348035835 60-61 0.01991277920258499 62-63 0.013981313057134142 64-65 0.017511947667521552 66-67 0.0032481838415564165 68-69 0.001835929997401453 70-71 1.4122538441549637E-4 72-73 0.0 74-75 0.0016947046129859565 76-77 0.0015534792285704603 78-79 0.009744551524669251 80-81 0.016099693823366585 82-83 0.016947046129859568 84-85 0.02866875303634577 86-87 0.01129803075323971 88-89 0.03996678378958547 90-91 0.046039475319451816 92-93 0.030081006880500732 94-95 0.030081006880500732 96-97 0.04745172916360678 98-99 0.05182971608048717 100-101 0.05818485837918451 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 354044.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.27337922839921 #Duplication Level Percentage of deduplicated Percentage of total 1 84.53622102277997 34.04559287786191 2 8.880652318176937 7.153077572110046 3 2.566782109838004 3.101189679185297 4 1.177426231489064 1.8967573253689611 5 0.6111005802378052 1.2305542707305965 6 0.41933519725680807 1.013282725376341 7 0.2677982651879047 0.7549598763433935 8 0.2023620489485398 0.6519842830992341 9 0.14964655154048953 0.5424095088371089 >10 0.958949988688426 7.428638942451252 >50 0.10116360478065602 2.943892589118549 >100 0.09343582941546702 7.96138333159124 >500 0.014050500663980003 3.6809267260386664 >1k 0.016158075763577 12.850706525493946 >5k 0.004215150199194001 11.897513432486699 >10k+ 7.02525033199E-4 2.847130333906772 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 10063 2.84230208674628 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 8614 2.433030922710172 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8470 2.3923580119985086 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7329 2.070081684762346 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7116 2.0099196710013443 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5465 1.5435934516613754 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5057 1.4283535379783303 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4673 1.3198924427472292 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3193 0.9018653048773599 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3109 0.8781394402955565 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3068 0.8665589587734859 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2600 0.7343719989605811 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2461 0.6951113420930732 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2445 0.6905921297917773 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2158 0.6095287591372823 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1968 0.5558631130593937 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1764 0.49824315621787124 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1742 0.4920292393035893 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1684 0.4756470947113918 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1667 0.47084543164126497 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1593 0.4499440747477715 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1404 0.3965608794387138 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1377 0.388934708680277 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1362 0.38469794714781214 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1277 0.3606896317971777 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1214 0.3428952333608252 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1193 0.33696376721537435 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1190 0.3361164149088814 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1168 0.32990249799459953 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1110 0.313520353402402 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 932 0.26324411655048524 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 881 0.2488391273401046 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 832 0.23499903966738597 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 822 0.23217453197907603 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 766 0.21635728892454045 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 725 0.20477680740246976 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 672 0.18980691665442712 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 663 0.1872648597349482 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 632 0.1785088859011874 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 631 0.17822643513235645 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 619 0.17483702590638453 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 606 0.17116516591158162 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 548 0.154783021319384 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 542 0.15308831670639808 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 534 0.15082871055575015 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 532 0.15026380901808817 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 523 0.14772175209860922 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 519 0.14659194902328523 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 517 0.14602704748562326 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 514 0.1451796951791303 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 491 0.13868332749601744 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 490 0.13840087672718646 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 486 0.13727107365186247 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 483 0.1364237213453695 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 480 0.13557636903887654 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 474 0.13388166442589056 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 471 0.1330343121193976 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 456 0.12879755058693268 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 447 0.12625549366745376 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 409 0.11552236445187604 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 396 0.11185050445707313 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 388 0.1095908983064252 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 383 0.10817864446227023 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 366 0.10337698139214334 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 358 0.10111737524149542 No Hit CTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG 356 0.10055247370383343 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 355 0.10027002293500242 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 355 0.10027002293500242 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 355 0.10027002293500242 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.4122538441549637E-4 0.0 16-17 0.0 0.0 0.0 2.8245076883099275E-4 0.0 18-19 0.0 0.0 0.0 2.8245076883099275E-4 0.0 20-21 0.0 0.0 0.0 8.473523064929782E-4 0.0 22-23 0.0 0.0 0.0 0.0015534792285704603 0.0 24-25 0.0 0.0 0.0 0.002259606150647942 0.0 26-27 0.0 0.0 0.0 0.0031069584571409205 0.0 28-29 0.0 0.0 0.0 0.007343719989605812 0.0 30-31 0.0 0.0 0.0 0.01595846843895109 0.0 32-33 0.0 0.0 0.0 0.0234434138129724 0.0 34-35 0.0 0.0 0.0 0.033752866875303635 0.0 36-37 0.0 0.0 0.0 0.043921094553219374 0.0 38-39 0.0 0.0 0.0 0.04914643377659274 0.0 40-41 0.0 0.0 0.0 0.0622803945272339 0.0 42-43 0.0 0.0 0.0 0.07993356757917094 0.0 44-45 0.0 0.0 0.0 0.1053541367739603 0.0 46-47 0.0 0.0 0.0 0.16777575668560968 0.0 48-49 0.0 0.0 0.0 0.28287444498423925 0.0 50-51 0.0 0.0 0.0 0.370716634090678 0.0 52-53 0.0 0.0 0.0 0.44570731321530654 0.0 54-55 0.0 0.0 0.0 0.5407519969269357 0.0 56-57 0.0 0.0 0.0 0.6091050829840359 0.0 58-59 0.0 0.0 0.0 0.70273751285151 0.0 60-61 0.0 0.0 0.0 0.8892962456643807 0.0 62-63 0.0 0.0 0.0 1.0130096824123556 0.0 64-65 0.0 0.0 0.0 1.1240128345629357 0.0 66-67 0.0 0.0 0.0 1.3287896419654055 0.0 68-69 0.0 0.0 0.0 1.5421811978172204 0.0 70-71 0.0 0.0 0.0 1.7189953791054218 0.0 72-73 0.0 0.0 0.0 1.9291387511156803 0.0 74-75 0.0 0.0 0.0 2.095502253957135 0.0 76-77 0.0 0.0 0.0 2.2000090384246027 0.0 78-79 0.0 0.0 0.0 2.341799324377761 0.0 80-81 0.0 0.0 0.0 2.5820237032685203 0.0 82-83 0.0 0.0 0.0 2.830721605224209 0.0 84-85 0.0 0.0 0.0 3.204686423156444 0.0 86-87 0.0 0.0 0.0 3.8646326445300585 0.0 88-89 0.0 0.0 0.0 4.444927749093333 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 50 0.0 85.495804 2 ATAACGC 60 3.6379788E-12 71.246506 3 GATAGTC 35 2.787921E-4 54.275383 7 CTTAACG 20 5.391827E-4 47.49096 20-21 AGATAGT 40 5.3881516E-4 47.49096 6 CATTCCG 40 5.3881516E-4 47.49096 9 TCGACCG 20 5.391827E-4 47.49096 18-19 TACCTGG 270 0.0 42.22015 2 TAACGCA 105 1.024091E-9 40.70654 4 GTCACTA 50 0.0016146944 37.998135 2 GACCGTC 25 0.0016169079 37.992767 20-21 CGACCGT 25 0.0016169079 37.992767 18-19 CGCTCCG 25 0.0016169079 37.992767 34-35 GTATAAC 120 3.774403E-9 35.628284 1 ATAGTCA 85 1.6311506E-5 33.52303 8 GACCCCG 30 0.003948154 31.669584 56-57 ACCTGGG 360 0.0 31.665115 3 CCGCCAG 30 0.0039508855 31.665113 38-39 AAGTTCG 30 0.003953619 31.660639 14-15 ACCGTCT 30 0.003953619 31.660639 20-21 >>END_MODULE