FastQCFastQC Report
Thu 26 May 2016
SRR936631_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936631_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2209299
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62400.28244253041349315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50200.227221394659573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30120.1363328367957438No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26300.1190422844531229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA110550.026.8143771
GTACATG91700.026.0579641
TACATGG91150.025.2261052
ACATGGG91850.023.9993973
GTACCTG23100.023.8554631
GGTATCA80000.023.5151121
TACCTGG22450.023.0659682
ATCAACG130400.022.3328653
TCAACGC130600.022.2627894
GAGTACT62750.022.14489712-13
CAACGCA132200.021.9938455
TATCAAC133100.021.8084432
AACGCAG135000.021.6080596
GTACTTT69050.019.98796514-15
AGTACTT67100.019.68266112-13
ACGCAGA148650.019.5288737
CATGGGG48150.019.4375384
CGCAGAG149650.019.4301228
CATGGGA45000.019.214554
AGAGTAC118850.018.52813510-11
Produced by FastQC (version 0.11.5)