##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936631_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2209299 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.405329020653156 31.0 31.0 34.0 30.0 34.0 2 31.486982522510534 33.0 31.0 34.0 28.0 34.0 3 31.500855248655796 33.0 31.0 34.0 28.0 34.0 4 35.12504826191475 37.0 35.0 37.0 32.0 37.0 5 35.12852719346725 37.0 35.0 37.0 32.0 37.0 6 35.11846065199867 37.0 35.0 37.0 32.0 37.0 7 35.02628933430921 37.0 35.0 37.0 32.0 37.0 8 34.98837549829154 37.0 35.0 37.0 32.0 37.0 9 36.48951318947775 39.0 37.0 39.0 32.0 39.0 10-11 36.35529233480847 39.0 37.0 39.0 32.0 39.0 12-13 36.25532714222928 39.0 35.0 39.0 32.0 39.0 14-15 37.370073041267844 40.0 37.0 41.0 32.0 41.0 16-17 37.264113865981926 40.0 37.0 41.0 31.5 41.0 18-19 37.21154877633131 39.5 37.0 41.0 31.0 41.0 20-21 37.063941322564304 39.0 36.0 41.0 31.0 41.0 22-23 37.17037349856221 39.0 37.0 41.0 31.0 41.0 24-25 37.210081116227364 40.0 37.0 41.0 31.0 41.0 26-27 37.12311869058919 39.5 36.5 41.0 31.0 41.0 28-29 36.975748416126564 39.0 36.0 41.0 30.5 41.0 30-31 36.7731418427293 39.0 36.0 41.0 30.0 41.0 32-33 36.58559796568957 39.0 35.5 41.0 30.0 41.0 34-35 36.39886543197639 39.0 35.0 41.0 30.0 41.0 36-37 36.19709826510581 39.0 35.0 40.0 30.0 41.0 38-39 35.99857601890916 39.0 35.0 40.0 29.0 41.0 40-41 35.78515764502677 38.0 35.0 40.0 28.0 41.0 42-43 35.54228286891 38.0 35.0 40.0 27.0 41.0 44-45 35.28515243975578 38.0 34.5 40.0 26.5 41.0 46-47 35.13057535444501 38.0 34.0 40.0 26.0 41.0 48-49 34.986368979481725 38.0 34.0 40.0 25.0 41.0 50-51 34.01491762771811 37.5 33.0 39.5 23.5 40.5 52-53 34.124515287428274 37.5 33.5 39.5 24.0 40.5 54-55 34.55637693223054 38.0 34.0 40.0 24.0 41.0 56-57 34.44075722661351 38.0 34.0 40.0 23.5 41.0 58-59 34.205445256617594 38.0 34.0 40.0 23.0 41.0 60-61 33.908286519841816 37.0 33.0 40.0 23.0 41.0 62-63 33.53732201933735 37.0 33.0 40.0 22.0 41.0 64-65 33.16339504068938 36.0 32.5 39.0 21.5 41.0 66-67 32.69208604177162 36.0 32.0 39.0 20.0 41.0 68-69 32.30293477704919 35.0 31.5 39.0 18.5 40.0 70-71 31.90489268315425 35.0 31.0 38.0 18.0 40.0 72-73 31.516385514138193 35.0 31.0 37.0 16.0 39.5 74-75 31.074911770656662 35.0 30.5 37.0 12.0 39.0 76-77 30.611880057882615 34.5 30.0 36.0 8.0 39.0 78-79 30.12175604116962 34.0 30.0 36.0 7.0 38.0 80-81 29.675207384785853 34.0 29.0 35.0 3.5 37.0 82-83 29.271052718531987 34.0 29.0 35.0 2.0 37.0 84-85 28.908698415198664 34.0 29.0 35.0 2.0 36.0 86-87 28.545999885031407 34.0 29.0 35.0 2.0 36.0 88-89 28.14094697005702 34.0 27.5 35.0 2.0 35.5 90-91 27.73950968157773 33.5 27.0 35.0 2.0 35.0 92-93 27.29174661283964 33.0 26.0 35.0 2.0 35.0 94-95 26.795713934600975 33.0 25.0 35.0 2.0 35.0 96-97 26.19928312102617 33.0 24.5 35.0 2.0 35.0 98-99 25.428554713508674 32.5 21.0 35.0 2.0 35.0 100-101 22.57833706528632 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33939764169253905 1101 2 0.35806952291200744 1101 3 0.3734657794116636 1101 4 0.4904379232567564 1101 5 0.34246765271934265 1101 6 0.47427237944898337 1101 7 0.5007114005055726 1101 8 0.4552250622741738 1101 9 0.583206183462174 1101 10-11 0.5595209778988561 1101 12-13 0.6032356190767203 1101 14-15 0.6546281071484685 1101 16-17 0.6791843361218568 1101 18-19 0.6841495882147584 1101 20-21 0.6860242005880721 1101 22-23 0.565457892912157 1101 24-25 0.4479287891489321 1101 26-27 0.4877435101486469 1101 28-29 0.4985223887709864 1101 30-31 0.4848019772246843 1101 32-33 0.3733698870857296 1101 34-35 0.37311794629817285 1101 36-37 0.29636454414271896 1101 38-39 0.28810387347386524 1101 40-41 0.21847058883010106 1101 42-43 0.31885885031818617 1101 44-45 0.2231813432269334 1101 46-47 0.40515262044592504 1101 48-49 0.5273097245285427 1101 50-51 0.3450657536724435 1101 52-53 0.4725936086722271 1101 54-55 0.3399706892613068 1101 56-57 0.18849594716170515 1101 58-59 0.19928136371994043 1101 60-61 0.3648267799097056 1101 62-63 0.40093919761446273 1101 64-65 0.2903905726760385 1101 66-67 0.4141671889572933 1101 68-69 0.2827766586617102 1101 70-71 0.19512100964238144 1101 72-73 0.1633204223416449 1101 74-75 0.2276006128628616 1101 76-77 0.337681914116299 1101 78-79 0.0235025814767873 1101 80-81 0.2871489119526558 1101 82-83 0.136554832710587 1101 84-85 0.03787839693790218 1101 86-87 0.034095978364639734 1101 88-89 -0.0023421051069441035 1101 90-91 0.011096814148981338 1101 92-93 -0.06139455654084358 1101 94-95 0.09702445363576473 1101 96-97 0.010355548142513271 1101 98-99 -0.06169504518442892 1101 100-101 0.29457176260421036 1102 1 0.2883824164856925 1102 2 0.3423762913267048 1102 3 0.41107028555703096 1102 4 0.38688844054169635 1102 5 0.37967152334472587 1102 6 0.3899602727509759 1102 7 0.27112689865197126 1102 8 0.4161609849039536 1102 9 0.48119343923571023 1102 10-11 0.48205693731522103 1102 12-13 0.5444941537232353 1102 14-15 0.4847001105480615 1102 16-17 0.3884603697667828 1102 18-19 0.3080310592976545 1102 20-21 0.25235739191954565 1102 22-23 0.11230753936981586 1102 24-25 0.2472480523178433 1102 26-27 0.015035747861873006 1102 28-29 -0.043495054201876826 1102 30-31 0.26937436487496313 1102 32-33 0.35722276928303387 1102 34-35 0.30562499970320545 1102 36-37 0.013630957249738174 1102 38-39 -0.007802852445983888 1102 40-41 0.027851194688267356 1102 42-43 0.07582583858559389 1102 44-45 0.12157214150642659 1102 46-47 0.23280825783166392 1102 48-49 0.31810464776825853 1102 50-51 0.46811979084169764 1102 52-53 0.48601648422046395 1102 54-55 0.4147326863923695 1102 56-57 0.29219395043232765 1102 58-59 0.39284738454242074 1102 60-61 0.4081754903178805 1102 62-63 0.5113705731896516 1102 64-65 0.4464649312628026 1102 66-67 0.30899085118878133 1102 68-69 0.331174671551814 1102 70-71 0.5050285727741901 1102 72-73 0.24792045278571706 1102 74-75 0.37074364080057975 1102 76-77 0.5684592051647925 1102 78-79 0.5351689768247638 1102 80-81 0.742640446280987 1102 82-83 0.62865866602084 1102 84-85 0.38515843588094256 1102 86-87 0.529147627577057 1102 88-89 0.4737685485209191 1102 90-91 0.4213765243383385 1102 92-93 0.28055909130370793 1102 94-95 0.19739435438598107 1102 96-97 0.26844199027711113 1102 98-99 0.2524737629618947 1102 100-101 0.2423518559871738 1103 1 0.1253843658451892 1103 2 0.1872838574121367 1103 3 0.18807721542293265 1103 4 0.26961117093028264 1103 5 0.3523877201657726 1103 6 0.3565239375468252 1103 7 0.394983256562945 1103 8 0.3314854140201007 1103 9 0.1201704539308821 1103 10-11 0.34530093393062344 1103 12-13 0.03051700514347999 1103 14-15 0.3152015618569095 1103 16-17 0.453529614871961 1103 18-19 0.5479152759121959 1103 20-21 0.4630254492007282 1103 22-23 0.34650085799368924 1103 24-25 0.4322488288484152 1103 26-27 0.2756479752728609 1103 28-29 0.35324017029578414 1103 30-31 0.10096697551718137 1103 32-33 0.1856772592363285 1103 34-35 0.4644016695116946 1103 36-37 0.5522869167201989 1103 38-39 0.5549363992998693 1103 40-41 0.3355341613563638 1103 42-43 0.34818710738299075 1103 44-45 0.5390387239150627 1103 46-47 0.5154734178505151 1103 48-49 0.45847968525593785 1103 50-51 0.41839773523140167 1103 52-53 0.6528484537162029 1103 54-55 0.5996645123638444 1103 56-57 0.5100830725779133 1103 58-59 0.37491233998563445 1103 60-61 0.5583297253549375 1103 62-63 0.5956011117269853 1103 64-65 0.5389893518103364 1103 66-67 0.7386042460733151 1103 68-69 0.5404764366861272 1103 70-71 0.7062688795305441 1103 72-73 0.3341741212232314 1103 74-75 0.30085602351768514 1103 76-77 0.3838848510240105 1103 78-79 0.3009765953929495 1103 80-81 0.37038682068654083 1103 82-83 0.21789367630317358 1103 84-85 0.31558852670823967 1103 86-87 0.4741631046345951 1103 88-89 0.373665055739977 1103 90-91 0.3319281020342366 1103 92-93 0.30008227475771676 1103 94-95 0.4087952958623511 1103 96-97 0.3848553026887487 1103 98-99 0.47931494371679406 1103 100-101 0.7617316490551644 1104 1 0.2714047717670809 1104 2 0.3164973837487359 1104 3 0.2130637615723856 1104 4 0.2962983936162189 1104 5 0.36653869982574605 1104 6 0.39911773010492624 1104 7 0.5102616139850795 1104 8 0.411944382521618 1104 9 0.4779072417005068 1104 10-11 0.5207282996117684 1104 12-13 0.49626336436678287 1104 14-15 0.3148768847493244 1104 16-17 0.27501761074245223 1104 18-19 0.2970295183231215 1104 20-21 0.11801723960621047 1104 22-23 0.33426416906868184 1104 24-25 0.35803313342162824 1104 26-27 0.1131979467177544 1104 28-29 0.24726652336875787 1104 30-31 0.29813246886163824 1104 32-33 0.22379860747689406 1104 34-35 0.31538160405459337 1104 36-37 0.3131628447613153 1104 38-39 0.19868379090027588 1104 40-41 0.16602896103127307 1104 42-43 0.35617196768259873 1104 44-45 0.4692428929613186 1104 46-47 0.16336418356680582 1104 48-49 0.29925830626371663 1104 50-51 0.16365044796697958 1104 52-53 0.373565187011458 1104 54-55 0.18209625466494117 1104 56-57 0.13636256122176604 1104 58-59 0.23216108246246137 1104 60-61 0.27889599517877883 1104 62-63 0.31961266556434964 1104 64-65 0.3261730490382959 1104 66-67 0.38498461113943705 1104 68-69 0.31960539596214943 1104 70-71 0.3622039496003886 1104 72-73 0.30978462401284546 1104 74-75 0.3032361195067921 1104 76-77 0.1830413769652317 1104 78-79 0.16318898501645762 1104 80-81 0.27254177060526885 1104 82-83 0.28658231027057823 1104 84-85 0.18601943432147294 1104 86-87 0.19436941531420615 1104 88-89 0.2050958590172236 1104 90-91 0.2476909353602501 1104 92-93 -0.056115694534558 1104 94-95 -7.340115663687641E-4 1104 96-97 0.07133459474164283 1104 98-99 0.13117981214362473 1104 100-101 0.08759351919530545 1105 1 0.21743266918479165 1105 2 0.13972924195864778 1105 3 0.26111922903202966 1105 4 0.24932679005464564 1105 5 0.1606835613332862 1105 6 0.19464171890594173 1105 7 0.3429413324873778 1105 8 0.39805023629604364 1105 9 0.4516896141063711 1105 10-11 0.4233078615875314 1105 12-13 0.18524017865946263 1105 14-15 0.2179975261330327 1105 16-17 0.25186373162368625 1105 18-19 0.3408029560984218 1105 20-21 0.28253564483149063 1105 22-23 -0.011174963476449307 1105 24-25 0.21197480028722282 1105 26-27 -0.10046447884120369 1105 28-29 -0.040580617151611875 1105 30-31 -0.036591501069807464 1105 32-33 -0.16744130554118186 1105 34-35 -0.007512221615527892 1105 36-37 -0.015829633552876032 1105 38-39 -0.03676915849402462 1105 40-41 -0.07382125318388688 1105 42-43 -0.17635375348189797 1105 44-45 -0.10716169689252553 1105 46-47 0.10576238731957943 1105 48-49 0.2437833158405951 1105 50-51 0.39898574669169307 1105 52-53 0.34934321600467655 1105 54-55 0.14445869716104198 1105 56-57 0.09685225513677409 1105 58-59 0.2625284537827497 1105 60-61 0.2419406126322201 1105 62-63 0.08736626527699087 1105 64-65 0.15767611875455856 1105 66-67 0.27595483699462164 1105 68-69 0.32810849864333136 1105 70-71 0.22155896816873977 1105 72-73 0.14897093606788658 1105 74-75 0.28616441057113917 1105 76-77 0.24100948169819603 1105 78-79 0.3318206167914646 1105 80-81 0.14132731446396107 1105 82-83 0.07637949643031305 1105 84-85 0.011956263300596959 1105 86-87 -0.11477334894226843 1105 88-89 -0.06169593226252701 1105 90-91 -0.13759440445694437 1105 92-93 -0.474385280529237 1105 94-95 -0.1119627733470665 1105 96-97 0.044346696278147135 1105 98-99 0.2090646974995849 1105 100-101 0.03819160518373721 1106 1 0.3880652148838344 1106 2 0.29720437901883656 1106 3 0.3462166119582477 1106 4 0.36363720367347696 1106 5 0.39554044241972264 1106 6 0.4563121538042054 1106 7 0.5171075188892331 1106 8 0.5251818731862912 1106 9 0.6719575279838068 1106 10-11 0.6008876224895374 1106 12-13 0.6388604517454226 1106 14-15 0.6897524137510658 1106 16-17 0.7428107399433443 1106 18-19 0.6359173699536882 1106 20-21 0.49483762795572517 1106 22-23 0.6232391503980281 1106 24-25 0.4628688317039291 1106 26-27 0.4675668787565854 1106 28-29 0.5784216142325818 1106 30-31 0.5254476294292232 1106 32-33 0.43592830625707535 1106 34-35 0.4790920845126436 1106 36-37 0.3250965017192442 1106 38-39 0.27710051980244543 1106 40-41 0.23241867972681973 1106 42-43 0.4250199885937249 1106 44-45 0.3857072942558162 1106 46-47 0.4905828791107467 1106 48-49 0.5232438676279187 1106 50-51 0.2380841049249085 1106 52-53 0.3662836336134063 1106 54-55 0.4545010343512743 1106 56-57 0.5088575823818289 1106 58-59 0.49208764457348764 1106 60-61 0.4998631348352305 1106 62-63 0.6682714905581264 1106 64-65 0.6026386950427778 1106 66-67 0.7064309641391802 1106 68-69 0.5877470439581671 1106 70-71 0.6240753710591953 1106 72-73 0.49796921450325726 1106 74-75 0.7021189922974322 1106 76-77 0.6284141676050119 1106 78-79 0.5875884124498683 1106 80-81 0.8508807051335232 1106 82-83 0.6862742445498924 1106 84-85 0.6401518714636225 1106 86-87 0.6939648845698727 1106 88-89 0.6267258067394081 1106 90-91 0.6562981048897498 1106 92-93 0.7090075317537199 1106 94-95 0.5918412933875778 1106 96-97 0.6610159489861012 1106 98-99 0.7652039595172866 1106 100-101 0.5885270516822345 1107 1 0.2616338975049892 1107 2 0.24577486528025005 1107 3 0.2156601116387442 1107 4 0.23523734178078826 1107 5 0.20454911019948696 1107 6 0.22454566853221536 1107 7 0.3380073292493435 1107 8 0.44607421132246117 1107 9 0.48603313658356484 1107 10-11 0.4590525731213262 1107 12-13 0.3563330879460338 1107 14-15 0.2007333621566545 1107 16-17 0.11672275837140234 1107 18-19 0.18317660974508243 1107 20-21 0.23012572083803917 1107 22-23 0.1915481603541238 1107 24-25 0.34251835863484814 1107 26-27 0.3352138239053062 1107 28-29 0.2129055412212253 1107 30-31 0.38676572973224665 1107 32-33 0.19821238475150693 1107 34-35 0.3380106991107965 1107 36-37 0.3151030463567608 1107 38-39 0.3772914480072558 1107 40-41 0.3330180036193795 1107 42-43 0.42053278928632665 1107 44-45 0.4912305560619359 1107 46-47 0.5071486707542263 1107 48-49 0.5306368602278368 1107 50-51 0.5160115419005677 1107 52-53 0.4810589258031257 1107 54-55 0.5734234108276866 1107 56-57 0.3401341216599647 1107 58-59 0.5124533197533339 1107 60-61 0.628826634309597 1107 62-63 0.5117541653051063 1107 64-65 0.4283510041145675 1107 66-67 0.6176834892355529 1107 68-69 0.672021491013588 1107 70-71 0.6234054475335462 1107 72-73 0.4196918713168962 1107 74-75 0.5766159054959559 1107 76-77 0.37165180493933647 1107 78-79 0.41433717051471675 1107 80-81 0.4224697637363697 1107 82-83 0.5390778745047484 1107 84-85 0.3745806585928442 1107 86-87 0.4698239326600735 1107 88-89 0.4088666761970785 1107 90-91 0.28953043436844794 1107 92-93 0.44872291732047387 1107 94-95 0.4902267776907543 1107 96-97 0.5874300029510167 1107 98-99 0.5329562887898547 1107 100-101 0.22360875658607782 1108 1 0.2616024427106005 1108 2 0.24967390688987123 1108 3 0.2898342372631042 1108 4 0.32784465336318647 1108 5 0.2835490100853022 1108 6 0.3138025371769899 1108 7 0.2582944540240888 1108 8 0.24449768349883527 1108 9 0.31000940140452116 1108 10-11 0.39682196821519256 1108 12-13 0.3096403059081396 1108 14-15 0.5575526601126981 1108 16-17 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-2.194219920659215 2316 60-61 -2.687055458007112 2316 62-63 -2.596947497674936 2316 64-65 -2.3820111404367736 2316 66-67 -2.3568163175496224 2316 68-69 -2.428865798115744 2316 70-71 -2.3429435250965795 2316 72-73 -2.204249611240467 2316 74-75 -2.0080313441909006 2316 76-77 -1.9679149274647934 2316 78-79 -2.7906021333788935 2316 80-81 -2.811851519210091 2316 82-83 -2.8393000536992865 2316 84-85 -2.523494355398576 2316 86-87 -2.2272751798842307 2316 88-89 -2.7549844950845355 2316 90-91 -2.6606551184924676 2316 92-93 -3.3842511918925418 2316 94-95 -3.120721840490095 2316 96-97 -3.3113320896321987 2316 98-99 -4.557397460910327 2316 100-101 -5.373320959854507 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5430.0 3 2222.0 4 2426.0 5 2492.0 6 2793.0 7 3346.0 8 4201.0 9 5189.0 10 6325.0 11 7868.0 12 9422.0 13 11034.0 14 12161.0 15 14639.0 16 17072.0 17 21214.0 18 17482.0 19 14586.0 20 15069.0 21 15863.0 22 17538.0 23 19891.0 24 22606.0 25 26219.0 26 30302.0 27 35379.0 28 42516.0 29 51283.0 30 62098.0 31 75729.0 32 94184.0 33 120822.0 34 160691.0 35 219429.0 36 308433.0 37 398707.0 38 293671.0 39 38960.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.04316618085568 17.668550910109047 13.891764722924771 23.396518186110498 2 15.853119175891308 19.137095981990278 40.198612150406355 24.81117269171206 3 19.891632457568345 23.299519593800532 29.016905662563993 27.791942286067133 4 12.92147471955257 15.65824505711263 36.33034101882356 35.089939204511246 5 14.308107258848473 36.52488611569043 33.752859842044806 15.414146783416289 6 33.38026242858747 34.54695385038962 17.32870050029525 14.744083220727655 7 30.074987506608824 31.539882888037486 19.882507803842895 18.502621801510795 8 27.160625177133824 33.92639020057208 19.242188681363757 19.67079594093034 9 26.599609702205502 16.643665358127656 19.98963129989088 36.767093639775965 10-11 25.68104423575407 25.760738914572055 26.86088773080043 21.697329118873444 12-13 26.601988782677104 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28.41440678081178 22.901489299970624 38-39 22.768426267432854 25.81606786045435 28.557367870389626 22.858138001723173 40-41 22.948110511992123 25.72740903355758 28.59001589897081 22.734464555479487 42-43 22.930557909976933 25.80902005046622 28.167294005229493 23.09312803432735 44-45 22.88782432184396 25.961295818547924 28.069052565254776 23.081827294353342 46-47 23.06407688855464 26.139926867427803 27.74289580452958 23.053100439487967 48-49 23.234031131367054 26.11419632523312 27.583473914387607 23.06829862901222 50-51 23.02912889431058 26.35483137243364 27.534041799584248 23.081997933671534 52-53 23.247457375478277 26.462102458183452 27.296736016527 22.99370414981127 54-55 22.927758650502586 26.1761840829485 27.497059992449678 23.398997274099234 56-57 23.00942476438089 26.59676022027026 27.282592656632055 23.111222358716798 58-59 23.251542142851484 26.17128734271614 27.32764269135755 23.249527823074825 60-61 23.108575003157185 26.170002720383913 27.80811742643534 22.91330485002356 62-63 22.938368139049352 26.25047668083036 27.739282460974408 23.071872719145876 64-65 22.827830832937465 26.37850687790861 27.560835899964854 23.232826389189075 66-67 22.824337890189536 26.54921981898578 27.27683042904014 23.349611861784542 68-69 22.81311815536383 26.30393778763292 27.59639120092029 23.28655285608296 70-71 22.879907801651285 26.3344476986074 27.41118946511599 23.374455034625324 72-73 22.9497168870065 26.26171790827755 27.49186061460961 23.296704590106344 74-75 22.757248216540194 26.677769736340203 27.20276216966689 23.362219877452716 76-77 22.962600938440005 26.516317664294913 27.16457379937812 23.35650759788697 78-79 22.984774047112758 26.52265755746669 27.203039801500832 23.289528593919716 80-81 22.965802581203924 26.74209975122891 27.12105440531388 23.171043262253292 82-83 22.963272403697893 26.689444523691463 27.139454372963634 23.207828699647013 84-85 22.971215669142605 26.517868965821652 27.00195004136623 23.508965323669518 86-87 23.015501138591226 26.520370144010286 27.138106814796707 23.32602190260178 88-89 22.959398833913085 26.486883050179493 26.92487302727381 23.628845088633607 90-91 22.829965485740935 26.50473226828526 27.219536270535645 23.445765975438164 92-93 22.917102428768178 26.361230257147177 27.38958354272551 23.332083771359134 94-95 22.752486197099064 26.373550760475162 27.2749577702416 23.599005272184176 96-97 22.747220709421363 26.331472248743392 27.284672603485767 23.63663443834948 98-99 22.735117358858485 26.402743015097634 27.004175748236026 23.857963877807855 100-101 22.748085102913688 26.141498789983427 27.232461327254338 23.877954779848547 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 166.0 1 135.5 2 197.0 3 301.5 4 258.0 5 220.5 6 362.5 7 747.0 8 1252.5 9 1521.0 10 1496.5 11 1434.5 12 1484.0 13 1638.5 14 1810.5 15 1956.0 16 2089.5 17 2280.5 18 2486.5 19 2748.5 20 3009.0 21 3442.5 22 4091.0 23 4931.0 24 6013.5 25 7390.5 26 9445.0 27 11898.0 28 14396.5 29 17419.5 30 21152.5 31 25450.0 32 30317.0 33 33987.0 34 37653.5 35 41591.0 36 45653.5 37 50250.0 38 52920.0 39 54543.0 40 57018.0 41 61793.5 42 66428.5 43 70092.5 44 73698.0 45 77997.5 46 81294.0 47 83999.0 48 92989.0 49 94774.5 50 87533.5 51 83816.5 52 82213.5 53 82435.5 54 81681.5 55 77178.5 56 70333.0 57 63333.0 58 57784.0 59 51674.0 60 44046.0 61 36767.5 62 30453.0 63 24046.0 64 17970.0 65 13863.5 66 10819.5 67 8751.0 68 6518.0 69 4405.0 70 3323.0 71 2556.0 72 2020.0 73 1581.0 74 1200.5 75 964.5 76 684.5 77 414.0 78 250.5 79 163.5 80 116.5 81 81.0 82 48.5 83 32.5 84 21.0 85 17.0 86 12.5 87 13.5 88 11.0 89 5.5 90 5.5 91 4.0 92 2.5 93 1.0 94 1.5 95 2.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012583176835729341 2 0.012221071027506915 3 0.015163180719314134 4 0.018920028479621816 5 0.03159373176740677 6 0.0453990157058868 7 0.0059294826096422445 8 0.023401087856374354 9 0.03299689177426867 10-11 0.03940163825720285 12-13 0.029941624017391942 14-15 0.027293725294765443 16-17 0.028221621428335415 18-19 0.023038982048151923 20-21 0.050649549925111996 22-23 0.01242475554463203 24-25 0.0025573722705708912 26-27 0.001041054198639478 28-29 6.789483904170508E-5 30-31 0.003168425821946237 32-33 0.0026705303356404 34-35 4.526322602780339E-5 36-37 2.715793561668203E-4 38-39 2.2631613013901695E-5 40-41 0.0016521077500148236 42-43 0.004458427763738634 44-45 0.0024668458185152847 46-47 0.0010636858116533797 48-49 7.242116164448543E-4 50-51 0.0030100045308489253 52-53 0.002987372917835024 54-55 0.0054768503493642096 56-57 5.88421938361444E-4 58-59 0.005273165832239094 60-61 0.002353687753445776 62-63 5.20527099319739E-4 64-65 7.015800034309525E-4 66-67 0.016136340078911907 68-69 0.03908479567500823 70-71 0.04601006925726214 72-73 0.012990545869979572 74-75 0.02718056722969593 76-77 0.023853720116652384 78-79 0.05107955057237613 80-81 0.07454853326779218 82-83 0.045896911192192635 84-85 0.01681528846932896 86-87 0.019417923965927654 88-89 0.020753189133747855 90-91 0.035825843401006384 92-93 0.02376319366459678 94-95 0.011428964572020356 96-97 0.01683792008234286 98-99 0.04852217830180523 100-101 0.0668990480690934 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2209299.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.760507170986465 #Duplication Level Percentage of deduplicated Percentage of total 1 80.86658917069377 48.32628382028461 2 11.305481865722172 13.512426602158948 3 3.2926075566867197 5.903036925078629 4 1.42978053002854 3.417776384708296 5 0.7445153444218499 2.224630728961571 6 0.4882539289753182 1.7506981454275095 7 0.3215764467113742 1.3452300084800797 8 0.23086519040760167 1.103729669350771 9 0.18053220046183976 0.9709826274264368 >10 1.009648369609746 11.247958048271775 >50 0.08464891758298558 3.5068129186963946 >100 0.042383471479565595 4.585902148317914 >500 0.002280736989074207 0.9133328925768809 >1k 6.842210967221926E-4 0.6796272566311742 >5k 1.520491326049317E-4 0.5115718236290542 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6240 0.28244253041349315 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5020 0.227221394659573 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3012 0.1363328367957438 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2630 0.1190422844531229 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.526322602780339E-5 0.0 0.0 0.0 6 0.0 4.526322602780339E-5 0.0 0.0 0.0 7 0.0 4.526322602780339E-5 0.0 0.0 0.0 8 0.0 4.526322602780339E-5 0.0 0.0 0.0 9 0.0 4.526322602780339E-5 0.0 0.0 0.0 10-11 0.0 4.526322602780339E-5 0.0 9.052645205560678E-5 0.0 12-13 0.0 4.526322602780339E-5 0.0 9.052645205560678E-5 0.0 14-15 0.0 4.526322602780339E-5 0.0 1.5842129109731187E-4 0.0 16-17 0.0 4.526322602780339E-5 0.0 1.8105290411121356E-4 0.0 18-19 0.0 4.526322602780339E-5 0.0 1.8105290411121356E-4 0.0 20-21 0.0 4.526322602780339E-5 0.0 1.8105290411121356E-4 0.0 22-23 0.0 4.526322602780339E-5 0.0 2.7157935616682036E-4 0.0 24-25 0.0 9.052645205560678E-5 0.0 3.3947419520852543E-4 0.0 26-27 0.0 1.3578967808341018E-4 0.0 4.526322602780339E-4 4.526322602780339E-5 28-29 0.0 1.3578967808341018E-4 0.0 9.278961335699695E-4 4.526322602780339E-5 30-31 0.0 1.3578967808341018E-4 0.0 0.002059476784265054 4.526322602780339E-5 32-33 0.0 1.3578967808341018E-4 0.0 0.003507900017154763 4.526322602780339E-5 34-35 0.0 1.3578967808341018E-4 0.0 0.0050468497021000785 4.526322602780339E-5 36-37 0.0 1.3578967808341018E-4 0.0 0.0075363271336292646 4.526322602780339E-5 38-39 0.0 1.3578967808341018E-4 0.0 0.011428964572020356 4.526322602780339E-5 40-41 0.0 1.5842129109731187E-4 0.0 0.01962160848305277 4.526322602780339E-5 42-43 0.0 1.8105290411121356E-4 0.0 0.026818461421473507 6.789483904170509E-5 44-45 0.0 2.0368451712511525E-4 0.0 0.035825843401006384 9.052645205560678E-5 46-47 0.0 2.2631613013901695E-4 0.0 0.051803762188820976 9.052645205560678E-5 48-49 0.0 2.2631613013901695E-4 0.0 0.06311956869577183 9.052645205560678E-5 50-51 0.0 2.2631613013901695E-4 0.0 0.07683432618219627 9.052645205560678E-5 52-53 0.0 2.2631613013901695E-4 0.0 0.09245013916178843 1.1315806506950849E-4 54-55 0.0 2.2631613013901695E-4 0.0 0.11361069732978651 1.3578967808341018E-4 56-57 0.0 2.2631613013901695E-4 0.0 0.1657086704877882 1.3578967808341018E-4 58-59 0.0 2.2631613013901695E-4 0.0 0.21332558426903736 1.3578967808341018E-4 60-61 0.0 2.4894774315291867E-4 0.0 0.2485630057316823 1.3578967808341018E-4 62-63 0.0 2.7157935616682036E-4 0.0 0.28848517108820493 1.3578967808341018E-4 64-65 0.0 2.7157935616682036E-4 0.0 0.3340652396982029 1.3578967808341018E-4 66-67 0.0 2.7157935616682036E-4 0.0 0.4094511426475095 1.3578967808341018E-4 68-69 0.0 2.7157935616682036E-4 0.0 0.501652334066145 1.3578967808341018E-4 70-71 0.0 2.9421096918072205E-4 0.0 0.5494276691384914 1.3578967808341018E-4 72-73 0.0 3.621058082224271E-4 0.0 0.601095641649229 1.3578967808341018E-4 74-75 0.0 3.621058082224271E-4 0.0 0.6956052575952825 1.3578967808341018E-4 76-77 0.0 3.847374212363288E-4 0.0 0.8125880652641404 1.3578967808341018E-4 78-79 0.0 4.073690342502305E-4 0.0 0.9293671884158732 1.3578967808341018E-4 80-81 0.0 4.073690342502305E-4 0.0 1.0200520617625772 1.3578967808341018E-4 82-83 0.0 4.073690342502305E-4 0.0 1.1642833315001728 1.5842129109731187E-4 84-85 0.0 4.526322602780339E-4 0.0 1.4023226371803905 1.8105290411121356E-4 86-87 0.0 4.526322602780339E-4 0.0 1.6215098092200286 2.0368451712511525E-4 88-89 0.0 4.526322602780339E-4 0.0 1.898407594445116 2.2631613013901695E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11055 0.0 26.814377 1 GTACATG 9170 0.0 26.057964 1 TACATGG 9115 0.0 25.226105 2 ACATGGG 9185 0.0 23.999397 3 GTACCTG 2310 0.0 23.855463 1 GGTATCA 8000 0.0 23.515112 1 TACCTGG 2245 0.0 23.065968 2 ATCAACG 13040 0.0 22.332865 3 TCAACGC 13060 0.0 22.262789 4 GAGTACT 6275 0.0 22.144897 12-13 CAACGCA 13220 0.0 21.993845 5 TATCAAC 13310 0.0 21.808443 2 AACGCAG 13500 0.0 21.608059 6 GTACTTT 6905 0.0 19.987965 14-15 AGTACTT 6710 0.0 19.682661 12-13 ACGCAGA 14865 0.0 19.528873 7 CATGGGG 4815 0.0 19.437538 4 CGCAGAG 14965 0.0 19.430122 8 CATGGGA 4500 0.0 19.21455 4 AGAGTAC 11885 0.0 18.528135 10-11 >>END_MODULE