FastQCFastQC Report
Thu 26 May 2016
SRR936631_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936631_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2209299
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69050.3125425757219824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59560.26958777422159697No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35870.16235919176173078No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35420.16032234659047961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG90150.024.401511
GTATCAA138050.024.3323731
TACCTGG19500.023.6313972
TACATGG92300.023.2642482
ACATGGG91650.022.7004763
GGTATCA102350.021.5392991
GAGTACT82300.021.09697512-13
AGTACTT85100.020.6261212-13
ATCAACG162800.020.5697023
GTACCTG22000.020.5164571
TCAACGC163300.020.4762444
TATCAAC166000.020.1759192
CAACGCA165650.020.1284085
GTACTTT85800.020.01581814-15
AACGCAG167650.019.9719226
CATGGGG45900.019.1435664
TACTTTT96000.018.30976514-15
AGAGTAC145800.018.13136310-11
ACGCAGA185000.018.0218647
CGCAGAG186900.017.8386558
Produced by FastQC (version 0.11.5)