##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936631_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2209299 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.968977037512804 31.0 31.0 34.0 30.0 34.0 2 32.0909071157865 33.0 31.0 34.0 30.0 34.0 3 32.21069081188196 34.0 31.0 34.0 30.0 34.0 4 35.92428050707487 37.0 35.0 37.0 35.0 37.0 5 35.7912627489534 37.0 35.0 37.0 35.0 37.0 6 35.8418498356266 37.0 35.0 37.0 35.0 37.0 7 35.77024567521191 37.0 35.0 37.0 33.0 37.0 8 35.735618402036124 37.0 35.0 37.0 33.0 37.0 9 37.36220267152613 39.0 37.0 39.0 34.0 39.0 10-11 37.26177918878341 39.0 37.0 39.0 33.5 39.0 12-13 37.1348554450982 39.0 37.0 39.0 33.0 39.0 14-15 38.26295625897626 40.0 38.0 41.0 33.0 41.0 16-17 38.23727503610874 40.0 37.5 41.0 33.0 41.0 18-19 38.35700056895875 40.0 38.0 41.0 33.5 41.0 20-21 38.35926373026014 40.0 38.0 41.0 34.0 41.0 22-23 38.258871931775644 40.0 38.0 41.0 33.5 41.0 24-25 38.141612792111886 40.0 37.5 41.0 33.0 41.0 26-27 37.94930812895855 40.0 37.0 41.0 33.0 41.0 28-29 37.97587515315944 40.0 37.0 41.0 33.0 41.0 30-31 37.87177471225036 40.0 37.0 41.0 33.0 41.0 32-33 37.70068424418786 40.0 37.0 41.0 33.0 41.0 34-35 37.52546033832451 40.0 36.5 41.0 32.5 41.0 36-37 37.35449570203038 39.5 36.0 41.0 32.0 41.0 38-39 37.161924891108 39.0 36.0 41.0 31.0 41.0 40-41 36.944571784987005 39.0 36.0 41.0 31.0 41.0 42-43 36.696177158456145 39.0 35.0 40.5 30.5 41.0 44-45 36.42605459921903 39.0 35.0 40.0 30.0 41.0 46-47 36.07313021008021 39.0 35.0 40.0 29.5 41.0 48-49 35.692207347217376 38.0 35.0 40.0 28.5 41.0 50-51 35.39227374836996 38.0 34.5 40.0 27.5 41.0 52-53 35.11566315831402 38.0 34.0 40.0 27.0 41.0 54-55 34.77393711761061 38.0 34.0 40.0 26.0 41.0 56-57 34.42825326042333 38.0 33.5 40.0 26.0 41.0 58-59 34.049018942207454 37.0 33.0 40.0 25.0 41.0 60-61 33.66785460003376 36.5 33.0 39.0 24.0 40.5 62-63 33.32988608603905 36.0 32.0 39.0 23.0 40.0 64-65 33.00332911027434 36.0 32.0 39.0 22.5 40.0 66-67 32.54587405326305 35.0 31.0 38.5 22.0 40.0 68-69 32.07794055942632 35.0 31.0 38.0 20.5 40.0 70-71 31.57179471859626 35.0 30.5 37.0 19.5 39.5 72-73 31.010705205587836 34.0 30.0 36.5 18.0 39.0 74-75 30.463608366273647 34.0 29.0 36.0 15.0 39.0 76-77 29.090542067868586 32.5 28.0 34.5 13.0 36.5 78-79 29.87924767086755 34.0 29.0 35.0 12.5 37.0 80-81 29.73535564900903 34.0 29.0 35.0 9.0 37.0 82-83 29.33546025232438 34.0 29.0 35.0 7.0 36.0 84-85 28.893996240436447 33.5 29.0 35.0 4.0 36.0 86-87 28.46058523540725 33.0 28.0 35.0 2.0 35.5 88-89 27.960022613507725 33.0 27.0 35.0 2.0 35.0 90-91 27.39510971579673 32.5 26.0 34.0 2.0 35.0 92-93 26.801121305898388 32.0 25.0 34.0 2.0 35.0 94-95 26.047719434988203 32.0 24.5 34.0 2.0 35.0 96-97 25.20324116382617 31.5 21.5 34.0 2.0 35.0 98-99 24.026383255503216 31.0 9.5 34.0 2.0 35.0 100-101 20.20419327578567 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21381182611897032 1101 2 0.345894524782409 1101 3 0.23076506518995643 1101 4 0.16973182217767402 1101 5 0.13746688471250224 1101 6 0.17754538527103136 1101 7 0.16279399941706174 1101 8 0.10211567536865118 1101 9 0.13628330000130262 1101 10-11 0.1623233638640187 1101 12-13 0.23254373863129985 1101 14-15 0.22881233624882924 1101 16-17 0.19510497578486508 1101 18-19 0.22037168296183296 1101 20-21 0.14247453560300727 1101 22-23 0.24925206893644258 1101 24-25 0.30068079141182835 1101 26-27 0.07013852990115765 1101 28-29 0.15230709292553257 1101 30-31 0.320061686008998 1101 32-33 0.280738212790439 1101 34-35 0.39655514086226873 1101 36-37 0.37673901446058977 1101 38-39 0.304003901924915 1101 40-41 0.350490364673 1101 42-43 0.3365361568247849 1101 44-45 0.3837006296677643 1101 46-47 0.3556157949031302 1101 48-49 0.35111878556519116 1101 50-51 0.27484055399387586 1101 52-53 0.32533366642203276 1101 54-55 0.39279121496932845 1101 56-57 0.38823835603356116 1101 58-59 0.3248713971122612 1101 60-61 0.4643551424440133 1101 62-63 0.3455486766511271 1101 64-65 0.3747512157447588 1101 66-67 0.22227247354946655 1101 68-69 0.2850921584458277 1101 70-71 0.14517292310554097 1101 72-73 0.3198563043514646 1101 74-75 0.19643284583508347 1101 76-77 0.21433815054848893 1101 78-79 0.31021398079805707 1101 80-81 0.41812491211970126 1101 82-83 0.24723614828923957 1101 84-85 0.13684611633467725 1101 86-87 0.32561959904638016 1101 88-89 0.03638053813374498 1101 90-91 -0.02259291846474909 1101 92-93 0.14573813346201447 1101 94-95 0.03581621958607073 1101 96-97 0.12005378917672616 1101 98-99 -0.4767180655316672 1101 100-101 -0.09122750620360165 1102 1 0.1910786673457423 1102 2 0.31275224209018404 1102 3 0.22462260385022148 1102 4 0.16637821746222414 1102 5 0.09998367916322337 1102 6 0.13093873641273746 1102 7 0.21090435283255715 1102 8 0.1309590543645669 1102 9 0.243998493601282 1102 10-11 0.21914595421049654 1102 12-13 0.26400300858669823 1102 14-15 0.26710632661032463 1102 16-17 0.37847262917065905 1102 18-19 0.3195999310429656 1102 20-21 0.2328964661165287 1102 22-23 0.2208213498115441 1102 24-25 0.11400379877865419 1102 26-27 0.1335047456502565 1102 28-29 0.24426219426306517 1102 30-31 0.3769258991090396 1102 32-33 0.17877607654687466 1102 34-35 0.2552872510063864 1102 36-37 0.41173238653258437 1102 38-39 0.32677458724421626 1102 40-41 0.3848157906521621 1102 42-43 0.39140855599730173 1102 44-45 0.5422681251249415 1102 46-47 0.417728703295019 1102 48-49 0.5436756858513476 1102 50-51 0.42669925439144407 1102 52-53 0.6016046271121738 1102 54-55 0.6662364531677625 1102 56-57 0.7455922905597774 1102 58-59 0.6747800120444438 1102 60-61 0.5328578702539417 1102 62-63 0.46866864954745324 1102 64-65 0.3389130999790595 1102 66-67 0.5456743976570451 1102 68-69 0.46194023513105975 1102 70-71 0.22789781546777732 1102 72-73 0.28026223058590105 1102 74-75 0.44297802515383466 1102 76-77 0.38110136438531583 1102 78-79 0.5205037597297029 1102 80-81 0.5916283693188262 1102 82-83 0.6451096352030419 1102 84-85 0.40495095704770634 1102 86-87 0.5605727754306571 1102 88-89 0.24392017620870732 1102 90-91 0.369479942231429 1102 92-93 0.6165576762301193 1102 94-95 0.7302952982723667 1102 96-97 0.5855811639992687 1102 98-99 0.41787660548737193 1102 100-101 0.48048334488789024 1103 1 0.15854310760108348 1103 2 0.23132417559774154 1103 3 0.1755731843737678 1103 4 0.10496991233391384 1103 5 0.08476689325001985 1103 6 0.14227527940575868 1103 7 0.19911742210979355 1103 8 0.13687086191351483 1103 9 0.18857349038805182 1103 10-11 0.18200181942621185 1103 12-13 0.2248943853486196 1103 14-15 0.164017877100882 1103 16-17 0.1684502972225843 1103 18-19 0.193066818708445 1103 20-21 0.13522271070758762 1103 22-23 0.1552245994580872 1103 24-25 0.10979326259769095 1103 26-27 0.1991874499045707 1103 28-29 0.1688995009337333 1103 30-31 0.18566347461994326 1103 32-33 0.2626290605664181 1103 34-35 0.14257798940662525 1103 36-37 0.17362521995874403 1103 38-39 0.1252545955986193 1103 40-41 0.2148019202095881 1103 42-43 0.449090049935009 1103 44-45 0.24952225948079132 1103 46-47 0.2549258563732977 1103 48-49 0.10348661404636772 1103 50-51 0.11929436599763932 1103 52-53 0.0697837432597268 1103 54-55 0.20380968803345212 1103 56-57 0.19598028807488532 1103 58-59 0.06478191320549342 1103 60-61 -0.18381009063034526 1103 62-63 -0.06319588067833593 1103 64-65 -0.07013160676015673 1103 66-67 -0.13665323933366125 1103 68-69 -0.08419116990728526 1103 70-71 -0.2010457548591127 1103 72-73 0.060955136845276314 1103 74-75 0.020861899023849162 1103 76-77 -0.06358301610395145 1103 78-79 -0.15227107853772281 1103 80-81 0.04962703582840078 1103 82-83 -0.013418946477258942 1103 84-85 -0.053309319932719035 1103 86-87 -0.17027998901816233 1103 88-89 -0.17183495960381023 1103 90-91 -0.13396223034044397 1103 92-93 -0.2563818400716684 1103 94-95 -0.20380055237670192 1103 96-97 -0.03416774885793572 1103 98-99 0.1260411549925351 1103 100-101 0.09215660760157007 1104 1 0.13139323719735785 1104 2 0.19956594952912354 1104 3 0.17558801106620336 1104 4 0.13656147539677477 1104 5 0.03916767522076725 1104 6 0.07528306616497105 1104 7 0.056022542260919295 1104 8 0.06028989719006006 1104 9 0.11922959898533492 1104 10-11 0.0893105550937392 1104 12-13 0.07250632687653535 1104 14-15 0.12530648141489564 1104 16-17 0.0828626659789009 1104 18-19 0.0485083521179277 1104 20-21 0.2076086824599983 1104 22-23 0.08885181247921992 1104 24-25 -0.01683122121376357 1104 26-27 -0.002853142284507726 1104 28-29 0.14105538438494847 1104 30-31 0.05843480309158622 1104 32-33 0.04462671026109177 1104 34-35 0.08505824794021066 1104 36-37 0.06748436088920329 1104 38-39 0.19956803736815232 1104 40-41 0.10086730043187941 1104 42-43 0.058493193193804416 1104 44-45 0.1472650328410694 1104 46-47 0.30135289806375454 1104 48-49 0.27047566975080173 1104 50-51 0.19781707829390172 1104 52-53 0.22630172199715304 1104 54-55 0.16062873099790664 1104 56-57 0.34391141534280933 1104 58-59 0.3506826896848665 1104 60-61 0.27191534648250837 1104 62-63 0.16701323059874085 1104 64-65 0.10159608730477032 1104 66-67 0.2611003794775755 1104 68-69 0.3710793074023826 1104 70-71 0.04038125937451298 1104 72-73 0.2808504988362266 1104 74-75 0.266373804308742 1104 76-77 0.19039040018563824 1104 78-79 0.2841701857947463 1104 80-81 0.29106585650230343 1104 82-83 0.28565012943540324 1104 84-85 0.22989464593294073 1104 86-87 0.24754457826207243 1104 88-89 0.256787558298047 1104 90-91 0.2523623424311623 1104 92-93 0.21933395969110236 1104 94-95 0.174766365428507 1104 96-97 0.13239050906061678 1104 98-99 0.0572043913849285 1104 100-101 -0.09807575961715997 1105 1 0.08715914305636119 1105 2 0.22644571736513797 1105 3 0.09336013933840093 1105 4 0.04344750656795071 1105 5 0.110872632349313 1105 6 0.10381335353952181 1105 7 0.15998623310451165 1105 8 0.06292623840201372 1105 9 0.04619950883196111 1105 10-11 0.07733320540526023 1105 12-13 0.05649944175475241 1105 14-15 0.16453829074578863 1105 16-17 0.1173093042957376 1105 18-19 0.15212733218323393 1105 20-21 -0.0019614038057937933 1105 22-23 0.042666962247949414 1105 24-25 0.0037354798955675506 1105 26-27 0.014029870891235419 1105 28-29 0.0369766004950165 1105 30-31 0.15324618121856304 1105 32-33 0.25001266697701396 1105 34-35 -0.09708900181909286 1105 36-37 0.1492942033055158 1105 38-39 -0.002136789037436415 1105 40-41 0.06331286568366323 1105 42-43 0.16560730752296848 1105 44-45 0.2002749007199398 1105 46-47 0.15907629508758703 1105 48-49 0.1448189289357913 1105 50-51 0.30214309909818127 1105 52-53 0.1719823510133338 1105 54-55 0.1301093231843069 1105 56-57 0.31090272965425214 1105 58-59 0.20781200766336383 1105 60-61 0.27473896255257557 1105 62-63 0.16911005539472512 1105 64-65 -0.018729287841715347 1105 66-67 0.14456682289215195 1105 68-69 0.24319629763680695 1105 70-71 0.2820377239662051 1105 72-73 0.09379687513876789 1105 74-75 0.015826757113202916 1105 76-77 -0.04630612246978316 1105 78-79 0.05014296927318185 1105 80-81 0.24629778746238884 1105 82-83 0.1862299142085675 1105 84-85 0.31712139230651815 1105 86-87 0.12330689208130963 1105 88-89 0.18526532914182425 1105 90-91 0.05274595750090327 1105 92-93 0.06790021293218373 1105 94-95 0.22600124277057887 1105 96-97 0.19624427325684834 1105 98-99 0.038742045851165585 1105 100-101 -0.0698232585457994 1106 1 0.12874822848439038 1106 2 0.1967653520683328 1106 3 0.10834255425763928 1106 4 0.09427245373882442 1106 5 0.18464315443410584 1106 6 0.12435575722795278 1106 7 0.16997574116557246 1106 8 0.13932898108351566 1106 9 0.15526395298085305 1106 10-11 0.17793390585301694 1106 12-13 0.12501752926638687 1106 14-15 0.2058392172874406 1106 16-17 0.26180528484870536 1106 18-19 0.1854420090758495 1106 20-21 0.198211122091557 1106 22-23 0.07368190956659504 1106 24-25 0.12775740213700004 1106 26-27 0.1199374974964229 1106 28-29 0.1675365892642091 1106 30-31 0.10255977197383004 1106 32-33 0.013602313945433764 1106 34-35 0.06335361761907166 1106 36-37 0.14652344478265888 1106 38-39 0.1282772731162254 1106 40-41 0.17323162704378348 1106 42-43 0.2817630463180265 1106 44-45 0.29110683020228834 1106 46-47 0.19111827023203887 1106 48-49 0.2895975268580955 1106 50-51 0.14466796203799248 1106 52-53 0.08466928485500347 1106 54-55 0.22135501927274248 1106 56-57 0.22070068594276648 1106 58-59 0.14126023511677488 1106 60-61 0.011273076131509185 1106 62-63 0.1273380999041933 1106 64-65 0.170226283968951 1106 66-67 -0.2145188637387534 1106 68-69 -0.042600055306103 1106 70-71 0.0025420759042589225 1106 72-73 -0.022780942537934834 1106 74-75 0.01721398354697712 1106 76-77 0.09187160506490599 1106 78-79 0.015328388433530904 1106 80-81 0.13491698918839035 1106 82-83 0.18429961910431203 1106 84-85 0.2639219050815633 1106 86-87 0.21301943512042243 1106 88-89 0.12103637582356797 1106 90-91 0.3037221880871215 1106 92-93 0.2184004272041733 1106 94-95 0.16183694048451613 1106 96-97 0.4197318085378363 1106 98-99 0.5505140752285911 1106 100-101 0.41628508180678736 1107 1 0.1295856328914553 1107 2 0.11515707181355594 1107 3 0.13993717837901443 1107 4 0.1089437789506249 1107 5 0.0225525807734428 1107 6 0.05948912354213576 1107 7 -0.024184844656787163 1107 8 -0.05824434977625259 1107 9 0.08842362152331162 1107 10-11 0.11433368529358034 1107 12-13 0.10620878212644413 1107 14-15 0.1522157040023373 1107 16-17 0.14266582738368783 1107 18-19 0.11039189364287694 1107 20-21 0.05512834015681989 1107 22-23 0.11261734955175484 1107 24-25 0.1794600385437377 1107 26-27 0.1384460673642991 1107 28-29 0.1762372210536327 1107 30-31 0.1917729725592494 1107 32-33 0.2300185322786632 1107 34-35 0.209856230320824 1107 36-37 0.25314821546268007 1107 38-39 0.19751129286350988 1107 40-41 0.19415514239017995 1107 42-43 0.282548273988958 1107 44-45 0.295286122350781 1107 46-47 0.4024366281979397 1107 48-49 0.2999143491531484 1107 50-51 0.24904333564919057 1107 52-53 0.17426575899596486 1107 54-55 0.2495536464448449 1107 56-57 0.41895940010185484 1107 58-59 0.3283415329132424 1107 60-61 0.18013825144533513 1107 62-63 -0.03333919278024666 1107 64-65 0.09392328271155748 1107 66-67 0.2459608015056105 1107 68-69 0.13678227520324526 1107 70-71 0.1854859517725842 1107 72-73 0.2843873680179989 1107 74-75 0.20197373866416513 1107 76-77 -0.051173171592896693 1107 78-79 0.12737953967134175 1107 80-81 0.3099035907178731 1107 82-83 0.46307154621663216 1107 84-85 0.109882652716216 1107 86-87 0.1950171728402097 1107 88-89 0.21677617752620293 1107 90-91 0.14345908850556555 1107 92-93 0.25825379125092596 1107 94-95 0.18305547984510184 1107 96-97 0.3027573635437122 1107 98-99 0.4823145720026538 1107 100-101 0.08846959935878473 1108 1 0.005784973761311818 1108 2 0.16936518214480145 1108 3 0.01587067550276089 1108 4 -0.028314797046867568 1108 5 0.01582666635212604 1108 6 -0.052391183186074386 1108 7 -0.024859600729925546 1108 8 0.02344003078752621 1108 9 0.040443898704310755 1108 10-11 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-1.7817894152858855 2316 96-97 -1.5984390693108033 2316 98-99 -1.486389622757894 2316 100-101 -1.1947949983546202 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 28.0 5 82.0 6 150.0 7 341.0 8 787.0 9 1527.0 10 2616.0 11 3985.0 12 5759.0 13 7914.0 14 10131.0 15 13118.0 16 16481.0 17 21175.0 18 19415.0 19 14524.0 20 13924.0 21 14693.0 22 15650.0 23 17850.0 24 20833.0 25 24757.0 26 30489.0 27 37148.0 28 45887.0 29 56446.0 30 70326.0 31 86604.0 32 108096.0 33 138457.0 34 183148.0 35 248593.0 36 348525.0 37 419800.0 38 202970.0 39 7054.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.693419633811466 18.794934962286547 14.422464923454953 23.089180480447038 2 16.215276614660617 20.147191030563548 38.99670544863931 24.64082690613652 3 20.099720709587764 23.69604874228783 29.114871195969528 27.08935935215488 4 13.5713037739658 16.392883880551885 35.6371311378205 34.39868120766182 5 14.273259840466704 37.016939209503555 33.45849210164431 15.251308848385428 6 33.40308396464218 35.30250998167292 16.617397645135405 14.677008408549499 7 30.05267300686057 31.92811630518569 19.488605167902282 18.530605520051456 8 27.280660778754267 34.52897606089246 18.690002860670845 19.500360299682427 9 26.61777632533049 16.67769581106759 19.5605572448805 37.143970618721426 10-11 25.835593099892773 26.247013192872494 26.320158566133422 21.59723514110132 12-13 26.689280174390156 23.28976295195897 27.156713509579284 22.86424336407159 14-15 23.087293615915694 25.00641608552246 25.520381878168152 26.385908420393694 16-17 22.883170948483087 28.31733927996319 26.079341157107766 22.720148614445957 18-19 22.714730466065983 26.690092329641896 28.51000200067172 22.085175203620402 20-21 23.38777317046344 25.772612817969943 28.665081843072592 22.174532168494025 22-23 22.9771225248281 25.758965206484795 28.565702752602533 22.69820951608457 24-25 23.09433207304968 25.949207234357086 28.694907099155344 22.261553593437895 26-27 22.91116906818365 26.1156485001118 28.615266025678743 22.357916406025804 28-29 22.47980295990149 26.364750045998807 28.76768690847969 22.387760085620016 30-31 22.671227404803755 25.992722101180398 28.660545699831825 22.675504794184018 32-33 22.656132948099852 26.100896106911797 28.61295577675952 22.63001516822883 34-35 23.20704030272306 26.026397740440327 28.31924880504056 22.44731315179606 36-37 23.243323250067558 25.845810676915598 28.376917091656022 22.533948981360822 38-39 22.888998129629528 26.08845421937834 28.495650890734513 22.526896760257614 40-41 23.007816513578756 26.061118084168854 28.435810124215983 22.495255278036407 42-43 23.002969293163382 26.14553157589803 28.05848511732329 22.793014013615295 44-45 23.011356370777573 26.27217402856825 27.918629386513356 22.797840214140816 46-47 23.30617087139405 26.567929465409616 27.41627547923572 22.709624183960614 48-49 23.535920479980806 26.555784642334178 27.24986477341909 22.658430104265918 50-51 23.248649673807904 26.78407590523795 27.2045068056078 22.76276761534635 52-53 23.537311568251855 26.88680697129573 26.888526982448646 22.687354478003773 54-55 23.1653936741373 26.69036351180727 27.086417366984183 23.057825447071252 56-57 23.25341684411803 27.09840764561876 26.869056284675448 22.779119225587763 58-59 23.482123882509264 26.65963259418992 26.888172053231724 22.970071470069094 60-61 23.258207712812546 26.70714225253742 27.43907048283273 22.595579551817305 62-63 23.098212485286606 26.739303665216507 27.40471910402248 22.757764745474407 64-65 22.9689107608519 26.80734521353907 27.211741152197995 23.012002873411035 66-67 23.051180336090486 26.987022051468212 26.97568354663 22.986114065811307 68-69 23.070544321132473 26.703983896844935 27.15594590306739 23.069525878955204 70-71 23.129395964465864 26.822634171336723 27.049797901909717 22.998171962287696 72-73 23.247195809777892 26.72859901726319 27.028725056167545 22.995480116791374 74-75 23.07809129089001 27.12635311734154 26.71730586947606 23.078249722292384 76-77 23.164708595514046 27.026926712819073 26.720061268982192 23.088303422684685 78-79 23.289584496575287 27.03057062096128 26.657307166264516 23.022537716198915 80-81 23.21162844646626 27.25698346148841 26.631994070352327 22.899394021693002 82-83 23.173252714393765 27.233153829754205 26.564507901129847 23.029085554722187 84-85 23.16708188790475 27.15236839055903 26.484951549475298 23.195598172060922 86-87 23.19048201005753 27.04706036781047 26.560297652186904 23.202159969945097 88-89 23.124173507005402 27.080204928393425 26.348240213519507 23.447381351081663 90-91 23.112958900271217 27.078847680293546 26.510478236533764 23.297715182901477 92-93 23.060067488508622 26.985649073334343 26.707819116312436 23.246464321844595 94-95 22.92625354044338 26.953425134969677 26.674014418643072 23.44630690594387 96-97 22.96508798952789 26.851844727078344 26.62874208402039 23.554325199373373 98-99 22.882970840818782 27.014801607939575 26.235500412576545 23.866727138665098 100-101 22.5956962001252 27.140934712439524 26.35666651729518 23.906702570140098 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 268.0 1 214.0 2 218.0 3 307.0 4 271.0 5 227.0 6 374.0 7 770.5 8 1248.0 9 1531.5 10 1547.5 11 1473.5 12 1571.5 13 1770.5 14 1978.0 15 2212.0 16 2408.5 17 2597.0 18 2830.0 19 3127.0 20 3399.0 21 3803.5 22 4462.0 23 5318.5 24 6358.5 25 7779.0 26 9849.5 27 12463.0 28 14968.5 29 17882.5 30 21603.0 31 25704.5 32 30373.0 33 34037.0 34 37728.5 35 41374.0 36 45700.5 37 50076.5 38 52764.0 39 54733.5 40 56966.5 41 61865.0 42 66573.5 43 70502.5 44 73919.0 45 77213.5 46 79758.0 47 83301.0 48 93029.5 49 94461.0 50 87470.0 51 83938.5 52 81856.0 53 81453.5 54 80172.0 55 76214.0 56 69768.0 57 62632.5 58 57886.5 59 52264.0 60 44200.5 61 36340.5 62 30268.0 63 24207.0 64 18016.0 65 13973.0 66 10712.5 67 8656.5 68 6541.0 69 4362.0 70 3211.5 71 2434.0 72 2026.5 73 1669.5 74 1319.0 75 1023.5 76 693.0 77 414.0 78 262.0 79 188.5 80 122.5 81 78.0 82 53.5 83 33.0 84 25.0 85 14.0 86 8.0 87 5.0 88 2.0 89 3.0 90 2.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012356860705590325 2 0.008916855527477268 3 0.0058389561575866375 4 2.2631613013901695E-4 5 0.004752638732919355 6 0.0 7 3.621058082224271E-4 8 0.0012221071027506915 9 4.526322602780339E-5 10-11 0.0 12-13 0.0 14-15 3.621058082224271E-4 16-17 0.004186848407571814 18-19 0.0018557922671399388 20-21 4.526322602780339E-4 22-23 3.621058082224271E-4 24-25 3.621058082224271E-4 26-27 4.526322602780339E-5 28-29 1.1315806506950847E-4 30-31 3.3947419520852543E-4 32-33 0.0034400051781130576 34-35 8.600012945282644E-4 36-37 0.001267370328778495 38-39 0.005024218089086176 40-41 9.052645205560678E-5 42-43 8.600012945282644E-4 44-45 0.004458427763738634 46-47 0.0 48-49 0.0019915819452233493 50-51 8.14738068500461E-4 52-53 4.978954863058373E-4 54-55 1.5842129109731187E-4 56-57 0.010998963924756223 58-59 0.0023763193664596777 60-61 0.002987372917835024 62-63 1.1315806506950847E-4 64-65 0.0035305316301686646 66-67 5.88421938361444E-4 68-69 0.001923687106181644 70-71 0.004503690989766437 72-73 0.0027384251746821048 74-75 0.0063821148699202785 76-77 0.001108949037681183 78-79 0.00613316712676736 80-81 0.0027836884007099087 82-83 0.0026252671096125965 84-85 0.0015842129109731185 86-87 4.073690342502305E-4 88-89 0.0017878974280982338 90-91 0.007807906489796085 92-93 0.0015389496849453153 94-95 2.94210969180722E-4 96-97 9.052645205560678E-5 98-99 5.431587123336406E-4 100-101 0.0019010554931677421 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2209299.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.53417157106887 #Duplication Level Percentage of deduplicated Percentage of total 1 79.38116137294978 45.671293579419974 2 12.209439295544321 14.049199504327945 3 3.5097471590768237 6.057911856641926 4 1.5145371965704735 3.4855057167300516 5 0.8368471102337802 2.407365260947175 6 0.510133275570771 1.7610057240480061 7 0.35266152181340804 1.4203061951768807 8 0.2585123955553495 1.18986372153036 9 0.19308194112711782 0.9997928575274354 >10 1.0901819051373143 11.686223137115215 >50 0.09258360335549193 3.7014993766830075 >100 0.04725671305854466 4.971805290342977 >500 0.0027546500050510446 1.1430952450535499 >1k 9.44451430303174E-4 0.872762547661283 >5k 1.574085717171957E-4 0.5823699867942181 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6905 0.3125425757219824 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5956 0.26958777422159697 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3587 0.16235919176173078 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3542 0.16032234659047961 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.8105290411121356E-4 0.0 12-13 0.0 0.0 0.0 1.8105290411121356E-4 0.0 14-15 0.0 2.2631613013901695E-5 0.0 2.0368451712511525E-4 0.0 16-17 0.0 4.526322602780339E-5 0.0 2.2631613013901695E-4 0.0 18-19 0.0 4.526322602780339E-5 0.0 3.621058082224271E-4 0.0 20-21 0.0 4.526322602780339E-5 0.0 3.621058082224271E-4 0.0 22-23 0.0 4.526322602780339E-5 0.0 4.752638732919356E-4 2.2631613013901695E-5 24-25 0.0 4.526322602780339E-5 0.0 5.431587123336407E-4 4.526322602780339E-5 26-27 0.0 4.526322602780339E-5 0.0 6.336851643892475E-4 4.526322602780339E-5 28-29 0.0 4.526322602780339E-5 0.0 0.0011315806506950847 4.526322602780339E-5 30-31 0.0 4.526322602780339E-5 0.0 0.002444214205501383 4.526322602780339E-5 32-33 0.0 4.526322602780339E-5 0.0 0.0040057955034606 4.526322602780339E-5 34-35 0.0 6.789483904170509E-5 0.0 0.005590008414433719 4.526322602780339E-5 36-37 0.0 9.052645205560678E-5 0.0 0.008215275524046314 4.526322602780339E-5 38-39 0.0 9.052645205560678E-5 0.0 0.01228896586654862 4.526322602780339E-5 40-41 0.0 9.052645205560678E-5 0.0 0.020526873003608836 4.526322602780339E-5 42-43 0.0 9.052645205560678E-5 0.0 0.028198989815321512 4.526322602780339E-5 44-45 0.0 9.052645205560678E-5 0.0 0.03731952985992389 4.526322602780339E-5 46-47 0.0 1.3578967808341018E-4 0.0 0.05384060736007213 4.526322602780339E-5 48-49 0.0 1.8105290411121356E-4 0.0 0.06522430870606469 4.526322602780339E-5 50-51 0.0 1.8105290411121356E-4 0.0 0.07898432941851691 4.526322602780339E-5 52-53 0.0 1.8105290411121356E-4 0.0 0.09435119465495617 4.526322602780339E-5 54-55 0.0 1.8105290411121356E-4 0.0 0.11544385798391255 4.526322602780339E-5 56-57 0.0 1.8105290411121356E-4 0.0 0.16765498920698374 4.526322602780339E-5 58-59 0.0 2.2631613013901695E-4 0.0 0.21522663976220513 4.526322602780339E-5 60-61 0.0 2.2631613013901695E-4 0.0 0.25066774574197515 4.526322602780339E-5 62-63 0.0 2.2631613013901695E-4 0.0 0.2910199117457619 4.526322602780339E-5 64-65 0.0 2.2631613013901695E-4 0.0 0.33732419197220476 4.526322602780339E-5 66-67 0.0 2.2631613013901695E-4 0.0 0.4122121994352055 4.526322602780339E-5 68-69 0.0 2.2631613013901695E-4 0.0 0.5042097063367159 4.526322602780339E-5 70-71 0.0 2.4894774315291867E-4 0.0 0.5523245156042709 4.526322602780339E-5 72-73 0.0 2.7157935616682036E-4 0.0 0.6044677519883004 4.526322602780339E-5 74-75 0.0 2.7157935616682036E-4 0.0 0.6988189466432565 4.526322602780339E-5 76-77 0.0 2.7157935616682036E-4 0.0 0.816118596894309 4.526322602780339E-5 78-79 0.0 2.7157935616682036E-4 0.0 0.9324903510117916 4.526322602780339E-5 80-81 0.0 2.7157935616682036E-4 0.0 1.0229036450023288 4.526322602780339E-5 82-83 0.0 2.7157935616682036E-4 0.0 1.1661617553803265 4.526322602780339E-5 84-85 0.0 2.9421096918072205E-4 0.0 1.4021868475023074 4.526322602780339E-5 86-87 0.0 3.621058082224271E-4 0.0 1.619133489853569 4.526322602780339E-5 88-89 0.0 3.621058082224271E-4 0.0 1.8927723228046545 4.526322602780339E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9015 0.0 24.40151 1 GTATCAA 13805 0.0 24.332373 1 TACCTGG 1950 0.0 23.631397 2 TACATGG 9230 0.0 23.264248 2 ACATGGG 9165 0.0 22.700476 3 GGTATCA 10235 0.0 21.539299 1 GAGTACT 8230 0.0 21.096975 12-13 AGTACTT 8510 0.0 20.62612 12-13 ATCAACG 16280 0.0 20.569702 3 GTACCTG 2200 0.0 20.516457 1 TCAACGC 16330 0.0 20.476244 4 TATCAAC 16600 0.0 20.175919 2 CAACGCA 16565 0.0 20.128408 5 GTACTTT 8580 0.0 20.015818 14-15 AACGCAG 16765 0.0 19.971922 6 CATGGGG 4590 0.0 19.143566 4 TACTTTT 9600 0.0 18.309765 14-15 AGAGTAC 14580 0.0 18.131363 10-11 ACGCAGA 18500 0.0 18.021864 7 CGCAGAG 18690 0.0 17.838655 8 >>END_MODULE