FastQCFastQC Report
Thu 26 May 2016
SRR936630_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936630_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2362576
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66100.27977935947880617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53180.22509328800428005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31990.13540305158437232No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26330.11144615030373627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG22300.026.4191972
GTACCTG23450.026.137741
GTACATG94050.025.7651421
GTATCAA124450.025.0073431
TACATGG94000.024.6152292
ACATGGG93000.023.6537953
GGTATCA92100.023.5764141
GTGTAGC13250.022.9501361
GAGTACT72950.021.42713712-13
ATCAACG145050.021.2256053
GTACACG4500.021.1173111
TCAACGC145500.021.0950974
TATCAAC146950.021.0481622
CAACGCA148150.020.7199555
AACGCAG150200.020.4687966
GTACTTT78550.019.68743714-15
AGTACAT67750.018.9346472
CATGGGG49200.018.5416374
ACGCAGA164850.018.5036757
AGTACTT78250.018.33648912-13
Produced by FastQC (version 0.11.5)