##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936630_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2362576 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.245441839754573 31.0 31.0 34.0 28.0 34.0 2 31.298384051983938 31.0 31.0 34.0 28.0 34.0 3 31.342966321506694 31.0 31.0 34.0 28.0 34.0 4 34.98118917655982 37.0 35.0 37.0 32.0 37.0 5 34.90247382518065 37.0 35.0 37.0 32.0 37.0 6 35.00860586072152 37.0 35.0 37.0 32.0 37.0 7 34.83513969497701 37.0 35.0 37.0 32.0 37.0 8 34.81735021434231 37.0 35.0 37.0 32.0 37.0 9 36.31356874868787 39.0 37.0 39.0 32.0 39.0 10-11 36.17628660411347 39.0 35.0 39.0 32.0 39.0 12-13 36.07421433215271 39.0 35.0 39.0 30.5 39.0 14-15 37.14714341464571 39.0 36.0 41.0 31.0 41.0 16-17 37.07865270789172 39.0 36.0 41.0 31.0 41.0 18-19 37.019615242007035 39.0 36.0 41.0 31.0 41.0 20-21 36.91195986922749 39.0 36.0 41.0 30.5 41.0 22-23 36.806222106717414 39.0 36.0 41.0 30.0 41.0 24-25 36.65468221974658 39.0 36.0 41.0 30.0 41.0 26-27 36.451991174040536 39.0 36.0 40.0 30.0 41.0 28-29 36.55165421133542 39.0 36.0 41.0 30.0 41.0 30-31 36.49549919240693 39.0 36.0 41.0 30.0 41.0 32-33 36.35828857992293 39.0 35.0 40.5 30.0 41.0 34-35 36.19443184049952 39.0 35.0 40.0 29.5 41.0 36-37 35.95942585550687 38.5 35.0 40.0 28.5 41.0 38-39 35.77761329159358 38.0 35.0 40.0 27.5 41.0 40-41 35.61971191614576 38.0 35.0 40.0 27.0 41.0 42-43 35.36190327845538 38.0 34.0 40.0 26.5 41.0 44-45 35.10845238417727 38.0 34.0 40.0 25.5 41.0 46-47 34.789540738583646 38.0 33.5 40.0 24.5 41.0 48-49 34.55950284773908 38.0 33.0 40.0 24.0 41.0 50-51 33.87427092292481 37.5 32.5 39.5 23.5 40.5 52-53 34.05990558610601 37.5 33.0 39.5 24.0 40.5 54-55 34.41183204265175 38.0 34.0 40.0 24.0 41.0 56-57 34.33420491023358 38.0 34.0 40.0 23.0 41.0 58-59 34.093119332457455 38.0 33.0 40.0 23.0 41.0 60-61 33.76804344071894 37.0 33.0 40.0 22.5 41.0 62-63 33.4399515613466 37.0 33.0 40.0 22.0 41.0 64-65 33.11685444193118 36.0 32.0 39.0 21.5 41.0 66-67 32.74609155430344 36.0 32.0 39.0 20.0 41.0 68-69 32.36200084145442 35.0 31.5 39.0 19.5 40.0 70-71 31.967675960477038 35.0 31.0 38.0 18.0 40.0 72-73 31.5562989296429 35.0 31.0 37.0 17.5 39.5 74-75 31.129883864053475 35.0 31.0 37.0 13.5 39.0 76-77 30.714776159581746 35.0 30.0 36.0 10.5 39.0 78-79 30.268702467137565 34.0 30.0 36.0 7.5 38.5 80-81 29.8629686410088 34.0 29.5 35.0 7.0 37.0 82-83 29.46944479246382 34.0 29.0 35.0 2.0 37.0 84-85 29.099749595356933 34.0 29.0 35.0 2.0 36.0 86-87 28.753721573401236 34.0 29.0 35.0 2.0 36.0 88-89 28.383840985432848 34.0 28.5 35.0 2.0 35.5 90-91 28.051239409864486 34.0 27.0 35.0 2.0 35.0 92-93 27.685444828018234 34.0 27.0 35.0 2.0 35.0 94-95 27.26217950237368 33.0 26.0 35.0 2.0 35.0 96-97 26.672952319840718 33.0 25.0 35.0 2.0 35.0 98-99 26.005401730991935 33.0 24.5 35.0 2.0 35.0 100-101 23.95364276112176 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22631712661042513 1101 2 0.23519020884478792 1101 3 0.2952469903985211 1101 4 0.2592261749159519 1101 5 0.3670793405912036 1101 6 0.1267856114060706 1101 7 0.223676679059011 1101 8 0.12269676744644187 1101 9 0.48254249190159015 1101 10-11 0.2622149662883899 1101 12-13 0.49251440874235186 1101 14-15 0.47723419054844385 1101 16-17 0.6126422144933912 1101 18-19 0.6285642230182731 1101 20-21 0.5436121396421214 1101 22-23 0.48468382419002154 1101 24-25 0.5725146907382523 1101 26-27 0.540782337946915 1101 28-29 0.6203189544585896 1101 30-31 0.3855296996934996 1101 32-33 0.28626708766707054 1101 34-35 0.34347947695608383 1101 36-37 0.373989247189634 1101 38-39 0.43270486198697 1101 40-41 0.3350387433252493 1101 42-43 0.1736443909378096 1101 44-45 0.18099458769836474 1101 46-47 0.3076003650650918 1101 48-49 0.21940908829785144 1101 50-51 0.3634132764348621 1101 52-53 0.3521024162068471 1101 54-55 0.47297112590128165 1101 56-57 0.2693352282603598 1101 58-59 0.2695169150154655 1101 60-61 0.387560677123453 1101 62-63 0.458881973181434 1101 64-65 0.4860770390246998 1101 66-67 0.3869542542038431 1101 68-69 0.3350105753133832 1101 70-71 0.3012594494419609 1101 72-73 0.25926945318509453 1101 74-75 0.13941632351519218 1101 76-77 0.1516856190164475 1101 78-79 0.0910100417368902 1101 80-81 0.11999214113381029 1101 82-83 0.36273213083405054 1101 84-85 0.3681945992725417 1101 86-87 0.2553919065020729 1101 88-89 0.19591491677798345 1101 90-91 0.3092103827318411 1101 92-93 0.12189584147209231 1101 94-95 -0.027583001694079456 1101 96-97 0.1267823731970772 1101 98-99 -0.03039541790127842 1101 100-101 0.06962826178855863 1102 1 0.07232900034829015 1102 2 0.039393802377102105 1102 3 0.10810036365779041 1102 4 0.1721239396845462 1102 5 0.133733216623952 1102 6 0.10795551099009515 1102 7 0.012289612842330655 1102 8 -0.054961298034996275 1102 9 0.14492933318807388 1102 10-11 0.19916242576627496 1102 12-13 0.3118537852992773 1102 14-15 0.16627625051265937 1102 16-17 0.08673888939098617 1102 18-19 0.033270862521568745 1102 20-21 -0.14741162220138904 1102 22-23 -0.31310238461654194 1102 24-25 -0.31999567884087554 1102 26-27 -0.17479324801110607 1102 28-29 -0.07285619779041497 1102 30-31 -0.09732308894544417 1102 32-33 -0.16520403753878554 1102 34-35 -0.2065808677519172 1102 36-37 -0.3246238649524287 1102 38-39 -0.39700056368599235 1102 40-41 -0.7210656094207764 1102 42-43 -0.3455465762818335 1102 44-45 -0.25717122301148976 1102 46-47 -0.24786245357775982 1102 48-49 -0.04316452184164632 1102 50-51 -0.07979723791211057 1102 52-53 -0.39360756058669466 1102 54-55 -0.2377910034841122 1102 56-57 -0.08873666913976308 1102 58-59 -0.13543992216876433 1102 60-61 -0.529966302113607 1102 62-63 -0.37364170641887995 1102 64-65 -0.21373435899075588 1102 66-67 -0.3609218523635249 1102 68-69 -0.3066068858093658 1102 70-71 -0.0376213568782795 1102 72-73 -0.2520674744072018 1102 74-75 -0.38308282680357664 1102 76-77 -0.34211282272266885 1102 78-79 -0.4127154265043558 1102 80-81 -0.4095076517718752 1102 82-83 -0.33348224200706866 1102 84-85 -0.4477144679905045 1102 86-87 -0.5381110914111318 1102 88-89 -0.6088379349801336 1102 90-91 -0.6496113521636531 1102 92-93 -0.6990471535873759 1102 94-95 -0.7272244634628855 1102 96-97 -0.8105159204051979 1102 98-99 -0.9701470731694961 1102 100-101 -0.7368001093403116 1103 1 0.2389270797275671 1103 2 0.3021228646721532 1103 3 0.29180008687306724 1103 4 0.3023212880886632 1103 5 0.40265747139815034 1103 6 0.210311847124089 1103 7 0.23979030092363018 1103 8 0.10188678610816737 1103 9 0.30479816047417785 1103 10-11 0.31892478942932456 1103 12-13 0.30163364850076846 1103 14-15 0.36286767055218405 1103 16-17 0.5324363716254865 1103 18-19 0.39489099394414495 1103 20-21 0.3050431275408201 1103 22-23 0.3765701114016693 1103 24-25 0.4086718764949282 1103 26-27 0.3831515891329005 1103 28-29 0.44582415251331753 1103 30-31 0.2534249794106458 1103 32-33 0.22089733863642635 1103 34-35 0.30524977679569787 1103 36-37 0.5157957277160605 1103 38-39 0.3809415627773589 1103 40-41 0.23054937196096148 1103 42-43 0.2928090627478781 1103 44-45 0.39871039242798645 1103 46-47 0.36904126514041025 1103 48-49 0.3411859540228832 1103 50-51 -0.1969903230515868 1103 52-53 0.07100600525942724 1103 54-55 0.16407087320288838 1103 56-57 0.19254604311580437 1103 58-59 0.17580660098578704 1103 60-61 0.33543250299110383 1103 62-63 0.21344250602427906 1103 64-65 0.12353526628034928 1103 66-67 0.16855490676789486 1103 68-69 0.17946181833510622 1103 70-71 0.31697813690084686 1103 72-73 0.15854360361964126 1103 74-75 0.08222014539213873 1103 76-77 -0.01181051747050077 1103 78-79 0.07408754651162752 1103 80-81 0.2390681871541318 1103 82-83 0.19927269921167223 1103 84-85 0.030655360468085036 1103 86-87 0.019193618749842045 1103 88-89 0.006838595005380199 1103 90-91 0.15977618418224893 1103 92-93 0.05764326182191226 1103 94-95 0.1932294266351704 1103 96-97 0.17914866315134503 1103 98-99 0.19131314323822224 1103 100-101 0.260801480925668 1104 1 0.09536261525980905 1104 2 0.11679893954265452 1104 3 0.21737055446183007 1104 4 0.25229743184849696 1104 5 0.18221548209116634 1104 6 0.15034726670015175 1104 7 0.23988869580864502 1104 8 0.43149957899274227 1104 9 0.37401470088677513 1104 10-11 0.36657296444308685 1104 12-13 0.2398645162158175 1104 14-15 0.209814491474134 1104 16-17 0.03278062459590103 1104 18-19 0.15980934362175248 1104 20-21 0.25306112269984027 1104 22-23 0.25671797489619763 1104 24-25 0.25179233727224215 1104 26-27 0.15916809416466293 1104 28-29 0.16927945289211266 1104 30-31 0.07967239606069398 1104 32-33 0.05916901052951573 1104 34-35 0.0778284007006178 1104 36-37 0.16062324935938221 1104 38-39 0.017831574211562895 1104 40-41 0.03135460884147534 1104 42-43 0.005575605387925009 1104 44-45 -0.16498764115927855 1104 46-47 -0.04716160910072631 1104 48-49 -0.15703983899534535 1104 50-51 -0.02750616662954286 1104 52-53 -2.3918717553073066E-4 1104 54-55 0.19399479794500252 1104 56-57 0.10055031205924791 1104 58-59 0.1897186305352463 1104 60-61 0.08767011501697652 1104 62-63 -0.08168171751805176 1104 64-65 -0.024435145525480095 1104 66-67 0.009086626528414854 1104 68-69 0.07205482700405952 1104 70-71 0.15992253281500624 1104 72-73 -0.14813937180552728 1104 74-75 -0.31838836628192624 1104 76-77 -0.1470717190889772 1104 78-79 -0.10519264712743848 1104 80-81 0.07766964977731305 1104 82-83 -0.15159726265062545 1104 84-85 -0.09832038364323381 1104 86-87 -0.025527308434714513 1104 88-89 -0.03122130836877446 1104 90-91 -0.19244005730100966 1104 92-93 -0.3624032110378188 1104 94-95 -0.24803496054999385 1104 96-97 -0.09651332330192375 1104 98-99 -0.08778143012891704 1104 100-101 0.13128140133169452 1105 1 0.15169895667153455 1105 2 0.20678183383386184 1105 3 0.114857117798838 1105 4 0.28584403087575794 1105 5 0.2874357271695658 1105 6 0.24594460980264188 1105 7 0.345470332820355 1105 8 0.10386907286578406 1105 9 0.4169095598653598 1105 10-11 0.3716633487372647 1105 12-13 0.17898654640135447 1105 14-15 0.30033835133011877 1105 16-17 0.25679589099536315 1105 18-19 0.17718652549088887 1105 20-21 -0.1262438745306298 1105 22-23 -0.08006959808034964 1105 24-25 -0.053720903312189705 1105 26-27 -0.01401634710057209 1105 28-29 0.05781552507543353 1105 30-31 -0.019535829467947963 1105 32-33 -0.044197863020933426 1105 34-35 -0.15806704783403802 1105 36-37 -0.1703694429180942 1105 38-39 -0.29443271063974663 1105 40-41 -0.424172198794885 1105 42-43 -0.035331994075733064 1105 44-45 0.08712786165678921 1105 46-47 -0.04032586153635975 1105 48-49 -0.026902102058826927 1105 50-51 0.0912157048445934 1105 52-53 0.2602670916297001 1105 54-55 0.12358410908866801 1105 56-57 0.29513015927641106 1105 58-59 0.19608874103244744 1105 60-61 0.357198252290452 1105 62-63 0.21605274985797251 1105 64-65 0.19798554663844214 1105 66-67 -0.13938029660285167 1105 68-69 0.10425792478124407 1105 70-71 0.23582091262511895 1105 72-73 0.2557509060648613 1105 74-75 0.0538790611079385 1105 76-77 0.23157691011061843 1105 78-79 0.18510291654560618 1105 80-81 0.39929863348958605 1105 82-83 0.3358152199614324 1105 84-85 0.12448592969030514 1105 86-87 0.15899912222906565 1105 88-89 0.29220202524404115 1105 90-91 0.33034433748633774 1105 92-93 0.16472026365913095 1105 94-95 0.2329297723771404 1105 96-97 0.35455774758410286 1105 98-99 0.24472773505835832 1105 100-101 0.38047275882252407 1106 1 0.0903863557177722 1106 2 0.03708847314909036 1106 3 0.11720442753662041 1106 4 0.25990258873547845 1106 5 0.35389895236279045 1106 6 0.21523595371477455 1106 7 0.4546165859966962 1106 8 0.41841508070731237 1106 9 0.3405020864303552 1106 10-11 0.3322733493358001 1106 12-13 0.3689567242737297 1106 14-15 0.29292900384294285 1106 16-17 0.28478424509022915 1106 18-19 0.24273395881180448 1106 20-21 0.27343494469838703 1106 22-23 0.3177901788621895 1106 24-25 0.05989263052698135 1106 26-27 0.17885820686479548 1106 28-29 -0.03282444629022763 1106 30-31 0.04160066021641029 1106 32-33 0.03599149043570549 1106 34-35 0.003240523265084505 1106 36-37 -0.06159399221855466 1106 38-39 0.10874709976260277 1106 40-41 -0.025826755544592572 1106 42-43 -0.01256679233543423 1106 44-45 -0.18213969993470158 1106 46-47 -0.01578129894857483 1106 48-49 -0.01721976458583896 1106 50-51 0.03555658088070146 1106 52-53 -0.11158269647274466 1106 54-55 0.17539904388171124 1106 56-57 0.0708531212529735 1106 58-59 0.16254850239437957 1106 60-61 0.13856759106629113 1106 62-63 0.20073398912391127 1106 64-65 0.10964504167022682 1106 66-67 -0.004162674434120106 1106 68-69 0.12363991888370407 1106 70-71 0.16612617171638888 1106 72-73 0.1684799419558587 1106 74-75 0.017929535367489535 1106 76-77 -0.08452330954406406 1106 78-79 0.28203312591894303 1106 80-81 0.1547226150667811 1106 82-83 0.17143306726592655 1106 84-85 0.17511533172552518 1106 86-87 0.28109340240842684 1106 88-89 0.24637022862641444 1106 90-91 0.32926006539057795 1106 92-93 0.24643018596977484 1106 94-95 0.2878648759715752 1106 96-97 0.37909905868273697 1106 98-99 0.5497564350904334 1106 100-101 0.5855790168243402 1107 1 0.4546041591015495 1107 2 0.4810638955305073 1107 3 0.6037414787421298 1107 4 0.4458854082970021 1107 5 0.4142937318800506 1107 6 0.41065238849535746 1107 7 0.6515942651964011 1107 8 0.6057855043391385 1107 9 0.6941931335069711 1107 10-11 0.8281151776589937 1107 12-13 0.7973229930244443 1107 14-15 1.0076177455022233 1107 16-17 0.9175751534233143 1107 18-19 1.0220152227650416 1107 20-21 1.1374894012817123 1107 22-23 1.0314158163450173 1107 24-25 1.0905567736430797 1107 26-27 1.0375731805352473 1107 28-29 0.8653014866680593 1107 30-31 0.8443977041793076 1107 32-33 1.0810058524668946 1107 34-35 0.8680984672351073 1107 36-37 0.9198312153651855 1107 38-39 1.139338681799991 1107 40-41 0.9958771068310099 1107 42-43 0.9179785024836065 1107 44-45 0.9990080205843199 1107 46-47 1.1441613744243142 1107 48-49 1.244320231249489 1107 50-51 1.017112229659432 1107 52-53 1.084877520987753 1107 54-55 1.329326488202895 1107 56-57 1.2444911076189058 1107 58-59 1.2652875649929882 1107 60-61 1.2551215236911375 1107 62-63 1.4309937476608923 1107 64-65 1.2602278421296305 1107 66-67 1.3119779300367966 1107 68-69 1.3899013628626662 1107 70-71 1.4412419235116012 1107 72-73 1.340417805372958 1107 74-75 1.1418993789831795 1107 76-77 1.2733887673531292 1107 78-79 1.3756387736683209 1107 80-81 1.5023720242339813 1107 82-83 1.5250809995620536 1107 84-85 1.7381559750419235 1107 86-87 1.715380233315127 1107 88-89 1.7694887789168057 1107 90-91 1.586440659115695 1107 92-93 1.5785482337325156 1107 94-95 1.6134379144346873 1107 96-97 1.9159106438858124 1107 98-99 2.046942837076788 1107 100-101 2.3801663653446496 1108 1 0.08972102199744114 1108 2 0.08583154867519127 1108 3 -0.03372548587987367 1108 4 0.06393694742649103 1108 5 0.2571682549650234 1108 6 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-2.1908057141794046 2316 70-71 -1.8705410823889856 2316 72-73 -1.8141979162769744 2316 74-75 -1.9375305012804382 2316 76-77 -2.547447854244716 2316 78-79 -2.398020616134314 2316 80-81 -2.3676971033611345 2316 82-83 -2.4774013134946635 2316 84-85 -2.6082415678623043 2316 86-87 -2.674377541361661 2316 88-89 -2.950300127567562 2316 90-91 -3.0111983669656865 2316 92-93 -3.4342492126578854 2316 94-95 -3.681841923408143 2316 96-97 -4.020246067396723 2316 98-99 -4.442048880851964 2316 100-101 -5.126050414473159 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6395.0 3 2575.0 4 2744.0 5 2864.0 6 3167.0 7 3651.0 8 4540.0 9 5696.0 10 6862.0 11 8624.0 12 10188.0 13 11890.0 14 13133.0 15 15187.0 16 17739.0 17 21204.0 18 19119.0 19 15098.0 20 15691.0 21 17072.0 22 18615.0 23 21289.0 24 24530.0 25 28437.0 26 33473.0 27 39320.0 28 46817.0 29 55996.0 30 68125.0 31 82654.0 32 103310.0 33 131557.0 34 172515.0 35 232905.0 36 324278.0 37 415586.0 38 313976.0 39 45740.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.02440449228919 17.687817226650637 13.904422441126544 23.38335583993362 2 15.866997790859264 19.157766681044354 40.15476260520297 24.820472922893412 3 19.88730114519036 23.285748996148836 28.946893881735626 27.880055976925178 4 12.9549371033233 15.623017939631046 36.22044110237487 35.20160385467078 5 14.3620618326206 36.48485392550461 33.73092211152839 15.422162130346392 6 33.36110121753772 34.53810940941649 17.27832948860366 14.822459884442122 7 30.10009542978544 31.545048023959904 19.840970115320165 18.51388643093449 8 27.237037466675755 33.94693268767101 19.191166540150608 19.624863305502625 9 26.614879223781017 16.652597979866396 19.884473080407414 36.848049715945166 10-11 25.713291211334237 25.80602679677314 26.77574883985242 21.704933152040205 12-13 26.626904741388348 23.024661469288603 27.324936765327646 23.023497023995407 14-15 23.191693467917453 24.625054135210387 25.680283268441638 26.50296912843052 16-17 22.953813379304535 27.939401162164273 26.215248267559843 22.891537190971352 18-19 22.780350659410146 26.414997280815637 28.427214875684903 22.37743718408931 20-21 23.449243892686294 25.593066746032235 28.419512498700822 22.538176862580652 22-23 23.032167964789608 25.520741392659644 28.43727418312233 23.00981645942842 24-25 23.068569747532788 25.682977881341046 28.56845651930065 22.679995851825513 26-27 22.86455446441402 25.865916786401584 28.5148785582117 22.754650190972697 28-29 22.50318779358124 26.07533668920256 28.717126044035467 22.704349473180734 30-31 22.63657726494348 25.72723426527767 28.619112594614055 23.017075875164792 32-33 22.505039063855797 25.84153001301141 28.606729531157544 23.046701391975255 34-35 23.23149036243411 25.82276343202149 28.188155389612646 22.75759081593176 36-37 23.207022173290166 25.60547598805398 28.295024740106655 22.8924770985492 38-39 22.82894277351315 25.85865127589732 28.414657633690375 22.897748316899158 40-41 22.999772063443825 25.734989573542766 28.461635653256224 22.803602709757186 42-43 22.954550697327534 25.847371627883454 28.03893919105816 23.15913848373086 44-45 22.98106464720046 25.98656064229218 27.956962394475266 23.0754123160321 46-47 23.15592982871994 26.193023930108083 27.58197337148493 23.069072869687048 48-49 23.291138276094213 26.175891019887544 27.44756269630458 23.085408007713667 50-51 23.053568042368173 26.41177162768018 27.433558459412964 23.101101870538677 52-53 23.290186505868864 26.471829415043814 27.17761252340159 23.060371555685734 54-55 22.9814073413605 26.246736268943504 27.356314881767062 23.415541507928932 56-57 23.060646457882587 26.6410598341898 27.142092523641594 23.156201184286022 58-59 23.283638189895512 26.247442068364315 27.189154560340523 23.279765181399654 60-61 23.161936970779518 26.254965492866834 27.67965190228376 22.903445634069893 62-63 23.008662081062383 26.302492001717642 27.580756885020076 23.1080890321999 64-65 22.88798189204433 26.444239623533996 27.41334117813881 23.254437306282863 66-67 22.880641923230037 26.556369444477955 27.171833700842598 23.391154931449417 68-69 22.83126003588208 26.392695242946917 27.481284872276174 23.294759848894827 70-71 22.93838429225824 26.398734494692107 27.26550996422458 23.39737124882507 72-73 23.02676399644696 26.34509405039231 27.338265007988415 23.289876945172317 74-75 22.82878926566526 26.720959148364294 27.108110539097673 23.34214104687277 76-77 23.007787243904012 26.644416390566416 27.00151427449744 23.34628209103213 78-79 23.090861169417195 26.53606809162859 27.056853461370324 23.316217277583895 80-81 22.99171689918943 26.818779995838447 27.021523446206835 23.16797965876529 82-83 23.03316081147004 26.72464896862543 27.03707191844241 23.205118301462115 84-85 22.999399553718355 26.58328287284656 26.897030341268096 23.52028723216699 86-87 23.07250942252534 26.52559405085624 27.070839104242083 23.33105742237634 88-89 23.01595028245909 26.533545879602077 26.840513260296657 23.609990577642176 90-91 22.924986935559556 26.54330250852072 27.12232866851498 23.409381887404738 92-93 22.993887489938245 26.43703462725626 27.2861272330854 23.282950649720092 94-95 22.84570618516663 26.430987654556148 27.170304732310797 23.55300142796643 96-97 22.788300325122655 26.387612577053137 27.19021812974684 23.633868968077365 98-99 22.721878811509644 26.456475602739776 26.951863371786583 23.869782213963994 100-101 22.687744491640522 26.275156822921836 27.21474996088557 23.822348724552068 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 155.0 1 134.0 2 209.5 3 303.5 4 256.0 5 233.0 6 393.5 7 816.0 8 1323.5 9 1628.0 10 1613.0 11 1491.5 12 1561.0 13 1741.0 14 1929.0 15 2093.0 16 2216.0 17 2390.0 18 2572.0 19 2792.5 20 3123.0 21 3659.0 22 4335.5 23 5182.0 24 6426.0 25 7938.5 26 10015.0 27 12553.5 28 15176.5 29 18409.5 30 22274.5 31 26930.5 32 32067.5 33 35920.5 34 39924.5 35 43873.5 36 48218.0 37 53326.0 38 56225.5 39 57833.0 40 60627.0 41 65761.5 42 70823.5 43 75078.0 44 78968.5 45 83440.0 46 86567.0 47 89872.5 48 99445.0 49 100931.0 50 93814.5 51 90115.5 52 88347.0 53 88270.0 54 87266.5 55 82627.5 56 75532.0 57 68103.5 58 62182.5 59 55842.5 60 47642.5 61 39817.5 62 33065.5 63 26216.5 64 19827.5 65 15364.0 66 11766.5 67 9373.0 68 7022.0 69 4823.5 70 3691.0 71 2834.0 72 2146.0 73 1728.5 74 1347.0 75 1047.0 76 764.0 77 452.0 78 272.0 79 170.0 80 106.5 81 77.5 82 59.0 83 41.5 84 25.5 85 20.5 86 17.5 87 13.0 88 13.0 89 11.0 90 5.5 91 3.5 92 2.5 93 1.5 94 2.0 95 1.5 96 0.5 97 0.5 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01295196429659829 2 0.005036875004232668 3 0.007364842443163733 4 0.03242223742220356 5 0.042622967472792414 6 0.05650611874496313 7 0.026665808845937656 8 0.023575961154265513 9 0.015068298331990167 10-11 0.043300194364117806 12-13 0.03963893648288986 14-15 0.018412106107909332 16-17 0.02190405726630593 18-19 0.027702812523279677 20-21 0.021607770501351067 22-23 0.01350221114580018 24-25 0.0038517279444132166 26-27 0.002772397586363359 28-29 0.002052844014330121 30-31 0.006327838765821713 32-33 0.0014391071440664766 34-35 0.002116334035391877 36-37 0.004931058302463074 38-39 0.005121528365648343 40-41 0.0032379910741495723 42-43 8.676969545106697E-4 44-45 0.001587250526543908 46-47 0.003005194330256466 48-49 6.983902316793195E-4 50-51 0.0020316806739762022 52-53 9.735136562802635E-4 54-55 0.002433784140700659 56-57 0.002349130779284984 58-59 0.003026357670610385 60-61 8.888602948645885E-4 62-63 0.001587250526543908 64-65 0.0033226444355652475 66-67 0.002137497375745796 68-69 0.004888731621755237 70-71 0.00844417280121359 72-73 0.003915217965474973 74-75 0.0026877442249476843 76-77 0.0024761108214084965 78-79 0.03210478731689478 80-81 0.020210990037992427 82-83 0.0031110110320260597 84-85 0.007047392337854951 86-87 0.006433655467591308 88-89 0.00465593487786213 90-91 0.010475853475189792 92-93 0.01225357406491897 94-95 0.005311998428833612 96-97 0.003513114498750516 98-99 0.020613093504716885 100-101 0.03875007618802527 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2362576.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.278559351327395 #Duplication Level Percentage of deduplicated Percentage of total 1 80.49996318292301 47.719218453185725 2 11.476728771938758 13.606478953329171 3 3.416290898695901 6.075384083992337 4 1.4310127406045612 3.3931349470573267 5 0.7845608065975327 2.3253817169308566 6 0.4989501826228465 1.7746228808378433 7 0.3295588488615973 1.3675041647398134 8 0.22998579425116297 1.0906581243583788 9 0.18065881916102122 0.963827508057959 >10 1.018066905025357 11.23073410164115 >50 0.08590524250967674 3.4933502432587846 >100 0.044881166456923825 4.742010330913356 >500 0.002649076937678114 1.054416051743372 >1k 6.443700659211176E-4 0.6570365036639736 >5k 1.431933479824706E-4 0.5062419362898958 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6610 0.27977935947880617 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5318 0.22509328800428005 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3199 0.13540305158437232 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2633 0.11144615030373627 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.2326680707837544E-5 0.0 8 0.0 0.0 0.0 4.2326680707837544E-5 0.0 9 0.0 0.0 0.0 4.2326680707837544E-5 0.0 10-11 0.0 0.0 0.0 1.2698004212351264E-4 0.0 12-13 0.0 0.0 0.0 1.2698004212351264E-4 0.0 14-15 0.0 0.0 0.0 1.6930672283135018E-4 0.0 16-17 0.0 0.0 0.0 2.1163340353918774E-4 0.0 18-19 0.0 0.0 0.0 2.3279674389310652E-4 0.0 20-21 0.0 0.0 0.0 2.539600842470253E-4 0.0 22-23 0.0 0.0 0.0 3.174501053087816E-4 0.0 24-25 0.0 1.2698004212351264E-4 0.0 5.502468492018881E-4 0.0 26-27 0.0 2.1163340353918774E-4 0.0 6.983902316793195E-4 0.0 28-29 0.0 2.1163340353918774E-4 0.0 0.0011428203791116138 0.0 30-31 0.0 2.1163340353918774E-4 0.0 0.002222150737161471 0.0 32-33 0.0 2.1163340353918774E-4 0.0 0.0036612578812279476 0.0 34-35 0.0 2.539600842470253E-4 0.0 0.00499454832352483 0.0 36-37 0.0 2.751234246009441E-4 0.0 0.007703455888826433 0.0 38-39 0.0 3.386134456627004E-4 0.0 0.011703327215717083 0.0 40-41 0.0 4.021034667244567E-4 0.0 0.018539086150032844 0.0 42-43 0.0 4.232668070783755E-4 0.0 0.024972741617624153 0.0 44-45 0.0 4.232668070783755E-4 0.0 0.03350156778025342 0.0 46-47 0.0 4.4443014743229424E-4 0.0 0.04812543596481129 0.0 48-49 0.0 4.65593487786213E-4 0.0 0.05832616601540014 0.0 50-51 0.0 4.65593487786213E-4 0.0 0.07123580363129059 0.0 52-53 0.0 4.65593487786213E-4 0.0 0.08480150479815252 0.0 54-55 0.0 4.65593487786213E-4 0.0 0.10226126059013552 0.0 56-57 0.0 5.079201684940506E-4 0.0 0.14623868184557873 0.0 58-59 0.0 5.079201684940506E-4 0.0 0.18809976906563006 2.1163340353918772E-5 60-61 0.0 5.290835088479693E-4 0.0 0.21897708264199756 4.2326680707837544E-5 62-63 0.0 5.502468492018881E-4 0.0 0.2558012948578162 4.2326680707837544E-5 64-65 0.0 5.502468492018881E-4 0.0 0.2941069408984092 4.2326680707837544E-5 66-67 0.0 5.925735299097257E-4 0.0 0.3609830964167925 4.2326680707837544E-5 68-69 0.0 5.925735299097257E-4 0.0 0.4410863396563751 4.2326680707837544E-5 70-71 0.0 6.56063550971482E-4 0.0 0.4804924793953718 4.2326680707837544E-5 72-73 0.0 7.407169123871571E-4 0.0 0.5273269515985941 4.2326680707837544E-5 74-75 0.0 7.618802527410758E-4 0.0 0.6122342730985162 4.2326680707837544E-5 76-77 0.0 8.465336141567509E-4 0.0 0.7202307989245638 4.2326680707837544E-5 78-79 0.0 8.888602948645885E-4 0.0 0.8225343861954071 4.2326680707837544E-5 80-81 0.0 9.100236352185072E-4 0.0 0.9042672066422414 4.2326680707837544E-5 82-83 0.0 9.31186975572426E-4 0.0 1.039585604865198 4.2326680707837544E-5 84-85 0.0 9.31186975572426E-4 0.0 1.26004412133197 4.2326680707837544E-5 86-87 0.0 9.31186975572426E-4 0.0 1.468164410372407 4.2326680707837544E-5 88-89 0.0 9.31186975572426E-4 0.0 1.739626577092123 4.2326680707837544E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2230 0.0 26.419197 2 GTACCTG 2345 0.0 26.13774 1 GTACATG 9405 0.0 25.765142 1 GTATCAA 12445 0.0 25.007343 1 TACATGG 9400 0.0 24.615229 2 ACATGGG 9300 0.0 23.653795 3 GGTATCA 9210 0.0 23.576414 1 GTGTAGC 1325 0.0 22.950136 1 GAGTACT 7295 0.0 21.427137 12-13 ATCAACG 14505 0.0 21.225605 3 GTACACG 450 0.0 21.117311 1 TCAACGC 14550 0.0 21.095097 4 TATCAAC 14695 0.0 21.048162 2 CAACGCA 14815 0.0 20.719955 5 AACGCAG 15020 0.0 20.468796 6 GTACTTT 7855 0.0 19.687437 14-15 AGTACAT 6775 0.0 18.934647 2 CATGGGG 4920 0.0 18.541637 4 ACGCAGA 16485 0.0 18.503675 7 AGTACTT 7825 0.0 18.336489 12-13 >>END_MODULE