FastQCFastQC Report
Thu 26 May 2016
SRR936630_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936630_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2362576
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70870.2999691861764447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64250.2719489235478562No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38330.16223816715314132No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34740.14704288877902763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA133650.026.8777561
CGAATCG908.9551747E-426.3864563
GTACATG92800.025.7716221
TACCTGG20950.024.7128932
GGTATCA96150.024.4741251
TACATGG95250.024.0360532
GTACCTG22200.023.5794661
ACATGGG95550.023.3128683
TCAACGC160450.022.3190634
ATCAACG160850.022.2640323
CAACGCA162550.022.0576065
TATCAAC162000.022.0194282
GAGTACT86950.021.73623312-13
AACGCAG166400.021.6328776
GCGTATC1404.50746E-420.5818121
GTACTTT91000.020.53402914-15
AGTACTT91000.019.8295612-13
ACGCAGA184100.019.4756327
CGCAGAG186550.019.1943978
CATGGGG49750.019.0933114
Produced by FastQC (version 0.11.5)