##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936630_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2362576 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.505696324689662 31.0 31.0 34.0 30.0 34.0 2 31.734051306709286 31.0 31.0 34.0 30.0 34.0 3 32.015785735569985 33.0 31.0 34.0 30.0 34.0 4 35.81143252111255 37.0 35.0 37.0 35.0 37.0 5 35.62068775776949 37.0 35.0 37.0 33.0 37.0 6 35.71990192061546 37.0 35.0 37.0 33.0 37.0 7 35.65417070181023 37.0 35.0 37.0 33.0 37.0 8 35.631737561035074 37.0 35.0 37.0 33.0 37.0 9 37.27243525710919 39.0 37.0 39.0 34.0 39.0 10-11 37.16051293164749 39.0 37.0 39.0 33.0 39.0 12-13 37.02915292460433 39.0 37.0 39.0 33.0 39.0 14-15 38.183169134029974 40.0 37.5 41.0 33.0 41.0 16-17 38.09489557161336 40.0 37.0 41.0 33.0 41.0 18-19 38.131120226396945 40.0 37.5 41.0 33.0 41.0 20-21 38.19302786450044 40.0 37.5 41.0 33.0 41.0 22-23 38.106716355368036 40.0 37.0 41.0 33.0 41.0 24-25 38.005244487373105 40.0 37.0 41.0 33.0 41.0 26-27 37.80459591564462 40.0 37.0 41.0 33.0 41.0 28-29 37.72535021095618 40.0 37.0 41.0 32.5 41.0 30-31 37.73520216915773 40.0 37.0 41.0 33.0 41.0 32-33 37.58720312066151 40.0 36.5 41.0 32.5 41.0 34-35 37.435313403674634 40.0 36.0 41.0 32.0 41.0 36-37 37.217387504148014 39.0 36.0 41.0 31.5 41.0 38-39 37.0687789514496 39.0 36.0 41.0 31.0 41.0 40-41 36.84193164579679 39.0 35.5 41.0 31.0 41.0 42-43 36.61422976446049 39.0 35.0 40.5 30.0 41.0 44-45 36.36772552502015 39.0 35.0 40.0 30.0 41.0 46-47 36.00386908188351 39.0 35.0 40.0 29.5 41.0 48-49 35.582228677511324 38.0 35.0 40.0 28.0 41.0 50-51 35.38959360460785 38.0 34.5 40.0 27.5 41.0 52-53 35.10550284943214 38.0 34.0 40.0 27.0 41.0 54-55 34.821972711142415 38.0 34.0 40.0 26.0 41.0 56-57 34.539100329470884 38.0 34.0 40.0 26.0 41.0 58-59 34.17044319420836 37.0 33.0 40.0 25.5 41.0 60-61 33.78541833151611 37.0 33.0 39.5 24.0 41.0 62-63 33.474106229810175 36.0 32.5 39.0 23.5 40.5 64-65 33.03235874740114 36.0 32.0 39.0 22.5 40.0 66-67 32.624144366149494 35.0 31.0 38.5 22.0 40.0 68-69 32.16065197479361 35.0 31.0 38.0 21.5 40.0 70-71 31.694729185431495 35.0 31.0 37.0 20.0 39.5 72-73 31.27287058702027 34.0 30.0 37.0 19.0 39.0 74-75 30.898253643480675 34.0 30.0 36.0 18.0 39.0 76-77 29.47961377750388 32.5 28.5 35.0 16.0 37.0 78-79 30.28681193747841 34.0 30.0 35.0 17.5 37.0 80-81 30.145593199964786 34.0 30.0 35.0 14.5 37.0 82-83 29.815108170065216 34.0 29.5 35.0 10.0 36.5 84-85 29.378738080806713 34.0 29.0 35.0 7.0 36.0 86-87 28.988531374228806 34.0 29.0 35.0 4.5 36.0 88-89 28.572605918285802 33.0 29.0 35.0 2.0 35.0 90-91 28.126486513026457 33.0 27.5 35.0 2.0 35.0 92-93 27.66825638624959 33.0 27.0 35.0 2.0 35.0 94-95 27.129886192020912 33.0 26.0 34.5 2.0 35.0 96-97 26.430131348155573 32.0 25.0 34.0 2.0 35.0 98-99 25.54057181652569 32.0 23.5 34.0 2.0 35.0 100-101 22.693811966260554 28.5 8.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.38173808899018624 1101 2 0.3171507647974465 1101 3 0.16702287912019287 1101 4 0.1301749072948013 1101 5 0.15291075410983268 1101 6 0.12382259003457818 1101 7 0.15706019733098486 1101 8 0.22830950756998192 1101 9 0.2394830334414948 1101 10-11 0.1895356172491489 1101 12-13 0.20080633972442996 1101 14-15 0.28829015261362656 1101 16-17 0.19537165326717343 1101 18-19 0.22094264373152583 1101 20-21 0.13113218486055445 1101 22-23 0.012348124493762214 1101 24-25 -0.02952844550603828 1101 26-27 0.019854241014492402 1101 28-29 0.06829075712165888 1101 30-31 0.0163851074619501 1101 32-33 0.09821838132457827 1101 34-35 0.16106328094090827 1101 36-37 0.16751468791325408 1101 38-39 0.23594526834828145 1101 40-41 0.3568451424260317 1101 42-43 0.4052332292257361 1101 44-45 0.3710168113950516 1101 46-47 0.4641674349474627 1101 48-49 0.3630089004240489 1101 50-51 0.4339942008266888 1101 52-53 0.32022916249615463 1101 54-55 0.24725426412072693 1101 56-57 0.23894288368543215 1101 58-59 0.3350937827577596 1101 60-61 0.23617369477609174 1101 62-63 0.06184162912884261 1101 64-65 0.2279893869852856 1101 66-67 0.0943415608747884 1101 68-69 -0.16549341092083125 1101 70-71 -0.1992933250843194 1101 72-73 -0.050301106909170556 1101 74-75 0.08610311808373439 1101 76-77 0.4562734794103598 1101 78-79 0.3758720614780664 1101 80-81 0.2813132144549577 1101 82-83 0.26758896585133485 1101 84-85 0.37777435573189777 1101 86-87 0.2108862469851438 1101 88-89 0.15064117434006263 1101 90-91 0.13444124432007598 1101 92-93 0.13323947563429073 1101 94-95 -0.2301996522175891 1101 96-97 -0.30225763606661715 1101 98-99 -0.21809895384694755 1101 100-101 -0.20802318604988557 1102 1 0.3538191243032358 1102 2 0.4398244218056533 1102 3 0.11534566286417558 1102 4 0.11709947740068571 1102 5 0.13288081956749664 1102 6 0.11539340539166432 1102 7 0.08705909745206952 1102 8 -0.02351099020945213 1102 9 0.06432062789730963 1102 10-11 0.09893872733018583 1102 12-13 0.15348188188546175 1102 14-15 0.05996259777326429 1102 16-17 0.16563008517674405 1102 18-19 0.004463745057130097 1102 20-21 0.08542589910210552 1102 22-23 0.05211531183583418 1102 24-25 0.0904017072285015 1102 26-27 -0.17983143885930275 1102 28-29 -0.04595117610875832 1102 30-31 0.0035072346475928384 1102 32-33 0.24930049868066817 1102 34-35 0.3543333101322901 1102 36-37 0.2867626803865093 1102 38-39 0.19174233622964465 1102 40-41 0.2773064326335444 1102 42-43 0.14944468292876678 1102 44-45 0.1010984749272339 1102 46-47 -0.08920394164513112 1102 48-49 -0.029201170007837618 1102 50-51 -0.009621646149440721 1102 52-53 -0.16783300196646422 1102 54-55 0.02980145239423848 1102 56-57 0.07571837560875139 1102 58-59 -0.031010389152328344 1102 60-61 0.04356415030432004 1102 62-63 -0.15500959342124787 1102 64-65 -0.17027261853987596 1102 66-67 -0.08957863052395965 1102 68-69 -0.22938225577052407 1102 70-71 -0.3600232260357643 1102 72-73 -0.3451439010177815 1102 74-75 -0.6621935559220624 1102 76-77 -0.33874278096169874 1102 78-79 -0.08023193207327495 1102 80-81 -0.14072630290628751 1102 82-83 -0.027481297584532882 1102 84-85 0.09654627899404034 1102 86-87 -0.014597538718810199 1102 88-89 -0.16560739383558243 1102 90-91 0.04186667797954513 1102 92-93 0.235657462816242 1102 94-95 0.004757274146012236 1102 96-97 -0.04278821121410914 1102 98-99 -0.5215522020569203 1102 100-101 -0.36494097852354557 1103 1 0.45298763437156 1103 2 0.4658388795262951 1103 3 0.3109658187686968 1103 4 0.11510268503946008 1103 5 0.0695508392502191 1103 6 0.07041036670603518 1103 7 0.14496759027451134 1103 8 0.1411144001607454 1103 9 0.07198637724845014 1103 10-11 0.17487646445952976 1103 12-13 0.2138969340376775 1103 14-15 0.21585064918635055 1103 16-17 0.1701959667682047 1103 18-19 0.14618899485808612 1103 20-21 0.10554746580989871 1103 22-23 0.1537291763868609 1103 24-25 0.07496899968633386 1103 26-27 0.09939542151250436 1103 28-29 0.2727253630890303 1103 30-31 0.34082841080535786 1103 32-33 0.1884766991064737 1103 34-35 0.14090355329472715 1103 36-37 0.15454172306446168 1103 38-39 0.3466474504183239 1103 40-41 0.30303953735099753 1103 42-43 0.38513678797041706 1103 44-45 0.2946407578225916 1103 46-47 0.3192086835140415 1103 48-49 0.3899117029615695 1103 50-51 0.28715983702446124 1103 52-53 0.22694955614122847 1103 54-55 0.1694411312568178 1103 56-57 0.18156603813254435 1103 58-59 0.19819488546877295 1103 60-61 0.08333196813951815 1103 62-63 0.03115195279742977 1103 64-65 0.04038014188075323 1103 66-67 0.03313784502584127 1103 68-69 0.009217521191438038 1103 70-71 0.00865029077834123 1103 72-73 -0.11436749166260185 1103 74-75 0.02107476776803452 1103 76-77 -0.08267267983235982 1103 78-79 0.06540026919754283 1103 80-81 0.0756674721695596 1103 82-83 0.1514376329989524 1103 84-85 0.33524347892907613 1103 86-87 0.21201391234211187 1103 88-89 0.2077718923493208 1103 90-91 -0.17227671475530215 1103 92-93 0.11647963699160613 1103 94-95 0.07870749428785828 1103 96-97 -0.19378656322627918 1103 98-99 -0.4131702171799425 1103 100-101 -0.09599596457956494 1104 1 0.20902836365585387 1104 2 0.3421885325939371 1104 3 0.06238960695885609 1104 4 0.02493836429020746 1104 5 -0.052296892728314504 1104 6 -0.014399180438608994 1104 7 0.05593248641432069 1104 8 -0.027105988886432897 1104 9 -0.07346979990366975 1104 10-11 -0.08810743144849909 1104 12-13 -0.1978380118354508 1104 14-15 0.015719585133467717 1104 16-17 -0.0916557423264166 1104 18-19 -0.01237203805577991 1104 20-21 -0.17065620901954048 1104 22-23 -0.03750625403588259 1104 24-25 -0.02649510845507308 1104 26-27 0.05655826569283562 1104 28-29 -0.23346550878390815 1104 30-31 -0.13962212671867036 1104 32-33 -0.16514799671839597 1104 34-35 -0.05115067235883686 1104 36-37 -0.005210055823376081 1104 38-39 -0.052175555648716454 1104 40-41 -0.004114235647357134 1104 42-43 0.006339077911214019 1104 44-45 -0.028709562186755022 1104 46-47 0.018739112648745504 1104 48-49 0.08840691976926962 1104 50-51 0.18058597403740606 1104 52-53 0.00453366472167005 1104 54-55 -0.009139709761200265 1104 56-57 0.07818742636557374 1104 58-59 0.13917771091276165 1104 60-61 -0.12946717910482164 1104 62-63 -0.25722344047542833 1104 64-65 -0.18473386124799873 1104 66-67 0.07526641563352854 1104 68-69 -0.08160302371220496 1104 70-71 -0.003678381710663814 1104 72-73 -0.13726735162421377 1104 74-75 -0.04646286149630541 1104 76-77 0.027502022739398058 1104 78-79 -9.299566910314638E-4 1104 80-81 0.029060707585781387 1104 82-83 0.009734278082017767 1104 84-85 0.15147282423507846 1104 86-87 0.1425851514655676 1104 88-89 0.019892928388060227 1104 90-91 -0.10011394482307523 1104 92-93 0.09461537673842813 1104 94-95 0.3243552961241747 1104 96-97 0.10361798114535858 1104 98-99 0.040079041468832344 1104 100-101 -0.0758173946497962 1105 1 0.3011357724545647 1105 2 0.35174079347216747 1105 3 0.0570598351692162 1105 4 0.009655459897984997 1105 5 -0.06962191902066905 1105 6 0.024209678483195773 1105 7 0.08436057674777686 1105 8 0.06731012943146197 1105 9 0.02077704385869339 1105 10-11 0.03242674728932826 1105 12-13 0.09222641680680255 1105 14-15 0.0982988962705349 1105 16-17 0.11781399390500269 1105 18-19 0.04294489289882364 1105 20-21 -0.06515973744333792 1105 22-23 -0.07686067362683957 1105 24-25 -0.17954617668711137 1105 26-27 -0.011794456531184494 1105 28-29 -0.017441599083042547 1105 30-31 -0.034338898829822995 1105 32-33 0.06417413262875726 1105 34-35 -0.021101996021158698 1105 36-37 0.1596201843761591 1105 38-39 0.11842837292059016 1105 40-41 -0.02287076747314387 1105 42-43 0.0276792913793642 1105 44-45 -0.12769767797741594 1105 46-47 0.09910781956675407 1105 48-49 0.10197562219794065 1105 50-51 -0.027414823480782502 1105 52-53 -0.018762250836481087 1105 54-55 0.04384745138435164 1105 56-57 0.050147465950999504 1105 58-59 0.020813996405784962 1105 60-61 0.020288118812459288 1105 62-63 -0.013457781423703352 1105 64-65 0.21792052751175106 1105 66-67 0.1447302497022136 1105 68-69 -0.1313339603537358 1105 70-71 -0.165593983424273 1105 72-73 -0.11065526565861816 1105 74-75 -0.0772213818359937 1105 76-77 -0.14572196433658036 1105 78-79 -0.10260150991711825 1105 80-81 -0.19198116277922495 1105 82-83 -0.10787971651361161 1105 84-85 -0.07063051114706909 1105 86-87 -0.08983693371439117 1105 88-89 -0.1614767034661888 1105 90-91 -0.21418076601989355 1105 92-93 0.0147307263887555 1105 94-95 0.18742300792698074 1105 96-97 -0.002725721464862829 1105 98-99 -0.05323085046349263 1105 100-101 0.07118626746393275 1106 1 0.26149374946560755 1106 2 0.2840362957071356 1106 3 0.06414555995069904 1106 4 0.010942386031715046 1106 5 -0.17419710930909105 1106 6 -0.06013063219194237 1106 7 -0.03940476978332441 1106 8 -0.0638505632764037 1106 9 -0.09078805181670901 1106 10-11 -0.14417708954749742 1106 12-13 0.015187623621550017 1106 14-15 0.07732407062666624 1106 16-17 0.05486330598944278 1106 18-19 -0.09361201101463479 1106 20-21 -0.08618963933197676 1106 22-23 -0.1255273042838141 1106 24-25 -0.04475629123376024 1106 26-27 -0.12115199014527889 1106 28-29 -0.08018226734236578 1106 30-31 -0.1753797389933851 1106 32-33 -0.03504393068079281 1106 34-35 -0.3361164173708886 1106 36-37 -0.2536196007388938 1106 38-39 -0.07060783128301296 1106 40-41 -0.05281081134038601 1106 42-43 -0.1769699665047284 1106 44-45 -0.17832993336025282 1106 46-47 -0.18708175233687996 1106 48-49 -0.30365163053659927 1106 50-51 -0.26323736861643 1106 52-53 -0.13097445920941908 1106 54-55 -0.1452661913465363 1106 56-57 -0.3840615044696065 1106 58-59 -0.39319161393920155 1106 60-61 -0.13308615054806694 1106 62-63 -0.5512423414686864 1106 64-65 -0.16433030987941777 1106 66-67 -0.2405924486515616 1106 68-69 -0.22199855013724346 1106 70-71 -0.24936024037234006 1106 72-73 -0.2195248768296203 1106 74-75 -0.33557707855778673 1106 76-77 -0.28732823182743417 1106 78-79 -0.17805092212087104 1106 80-81 -0.2918086480549533 1106 82-83 -0.14662545656515036 1106 84-85 -0.11818964478579375 1106 86-87 -0.13565857772304568 1106 88-89 -0.19828727661878887 1106 90-91 -0.21929038168193316 1106 92-93 0.0560381753911372 1106 94-95 0.1319087411225226 1106 96-97 0.11907804811258416 1106 98-99 0.12805639152918147 1106 100-101 0.16877599827911993 1107 1 0.6019159064466102 1107 2 0.5524633503922018 1107 3 0.38312929310682975 1107 4 0.21731166252351386 1107 5 0.10561916603961663 1107 6 0.21582758747642572 1107 7 0.22801469838783817 1107 8 0.2110297025063943 1107 9 0.34660051208479814 1107 10-11 0.3845749003979009 1107 12-13 0.37339686344807177 1107 14-15 0.49993498023548 1107 16-17 0.44970694698962177 1107 18-19 0.5532910990206972 1107 20-21 0.45028209002801844 1107 22-23 0.4680917588277893 1107 24-25 0.37199623937605253 1107 26-27 0.4205215248247427 1107 28-29 0.40235692154627145 1107 30-31 0.403562495975585 1107 32-33 0.3576761175402723 1107 34-35 0.4312390958029937 1107 36-37 0.428429311503038 1107 38-39 0.3858593182916934 1107 40-41 0.37357681804192566 1107 42-43 0.4287025042607482 1107 44-45 0.42317373468664243 1107 46-47 0.43314014281674673 1107 48-49 0.5368817305161997 1107 50-51 0.4642773030649394 1107 52-53 0.1453369472909074 1107 54-55 0.2402737693894892 1107 56-57 0.414832681565521 1107 58-59 0.21199317887866442 1107 60-61 0.188729489638078 1107 62-63 0.28435036948409476 1107 64-65 0.2394004601691222 1107 66-67 0.20084114226797567 1107 68-69 0.2100841114436207 1107 70-71 0.2735455203360715 1107 72-73 0.2731842534802915 1107 74-75 0.43652224169677467 1107 76-77 0.36189185469912033 1107 78-79 0.3803070004183198 1107 80-81 0.3487144025747888 1107 82-83 0.29116531508311283 1107 84-85 0.514962887713974 1107 86-87 0.7707081000696547 1107 88-89 0.6630493457001236 1107 90-91 0.5708445009260537 1107 92-93 0.6730518583856231 1107 94-95 0.881853898446753 1107 96-97 0.9590426484309766 1107 98-99 1.2979595916752515 1107 100-101 1.2466115924244185 1108 1 0.36282802170363126 1108 2 0.3300846298246185 1108 3 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-1.2065238529557263 2316 56-57 -1.0277563977378321 2316 58-59 -1.40570208305882 2316 60-61 -1.2077483217627503 2316 62-63 -1.081176380003292 2316 64-65 -1.205453166329324 2316 66-67 -1.2104926562132405 2316 68-69 -1.3283252134647832 2316 70-71 -1.2087065619410993 2316 72-73 -1.0564975818852353 2316 74-75 -1.3696591729451022 2316 76-77 -1.1282276726270126 2316 78-79 -1.0881402505925486 2316 80-81 -0.908080545605646 2316 82-83 -0.9615047121463327 2316 84-85 -1.1884648340544217 2316 86-87 -1.04262082568264 2316 88-89 -1.2472295949473597 2316 90-91 -1.342265178156751 2316 92-93 -1.3854091292957982 2316 94-95 -1.4959796709447737 2316 96-97 -1.5388260770812536 2316 98-99 -1.5985047182421575 2316 100-101 -2.4218747257820255 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 15.0 4 29.0 5 69.0 6 164.0 7 438.0 8 1002.0 9 1801.0 10 2953.0 11 4492.0 12 6431.0 13 8807.0 14 11141.0 15 14188.0 16 17283.0 17 21832.0 18 19812.0 19 14235.0 20 13473.0 21 14526.0 22 15251.0 23 17470.0 24 20564.0 25 24668.0 26 30555.0 27 37937.0 28 47628.0 29 58610.0 30 72537.0 31 89618.0 32 111663.0 33 143061.0 34 185660.0 35 251595.0 36 356495.0 37 458669.0 38 272662.0 39 15240.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.51497502023883 18.85094810131565 14.42420751943013 23.209869359015393 2 16.12220319646784 20.15345246889148 39.05325118158401 24.671093153056667 3 20.14172521089255 23.689173018452383 29.087319610626043 27.08178216002902 4 13.558535676792305 16.38313183649971 35.642673304209424 34.41565918249856 5 14.275985195820157 36.96384793547382 33.524720474600606 15.235446394105418 6 33.462076987153004 35.24009386364714 16.63684046566121 14.660988683538644 7 30.07674673745945 31.9164335877449 19.516959454426015 18.489860220369632 8 27.35763844210726 34.506530160299604 18.71376836131409 19.422063036279045 9 26.64108337047652 16.674476259894796 19.547348357382223 37.137092012246455 10-11 25.79202910727339 26.277305528595758 26.341155461571315 21.58950990255954 12-13 26.7047000915528 23.234582156472758 27.168961271428454 22.891756480545983 14-15 23.107492740437493 24.961128193472078 25.49014877209111 26.44123029399932 16-17 22.914352808121304 28.25346147594829 26.087541734107177 22.74464398182323 18-19 22.770044809150015 26.62187938544186 28.455492745099576 22.152583060308547 20-21 23.42350045035588 25.742494632976886 28.590551161105505 22.243453755561728 22-23 22.989133114741332 25.73286979356368 28.548393479995827 22.729603611699165 24-25 23.09352164755758 25.86086119557635 28.696283209513684 22.34933394735238 26-27 22.927859871568128 26.072394300203317 28.577118487853614 22.42262734037494 28-29 22.5298149138906 26.30910074427235 28.714801132323366 22.446283209513684 30-31 22.709110733369002 25.951821232417497 28.676961079770557 22.662106954442947 32-33 22.643210964806922 26.062686576640736 28.642468097444727 22.651634361107615 34-35 23.219186938944127 26.00926831446578 28.32262216645422 22.448922580135875 36-37 23.31120356012171 25.810713084657866 28.34764054317803 22.530442812042395 38-39 22.905408637108778 26.06605171565069 28.477088327006193 22.551451320234335 40-41 23.093400280172695 25.948757477074324 28.407771268351812 22.550070974401173 42-43 23.004085001904922 26.092431105278752 28.095246158404947 22.808237734411378 44-45 23.043896017591422 26.186331092077726 27.931801491207466 22.83797139912339 46-47 23.366035480517546 26.48325782125648 27.429888899047466 22.720817799178512 48-49 23.542460947975293 26.488493666950358 27.225287581601126 22.743757803473226 50-51 23.298610427707384 26.66245936878257 27.207672689155395 22.831257514354654 52-53 23.583988425898493 26.764030381169906 26.935711758142588 22.716269434789012 54-55 23.198057665541217 26.613355898797447 27.110474822970037 23.078111612691295 56-57 23.282291771825996 26.980937245567326 26.86980433538985 22.86696664721682 58-59 23.5688221229038 26.543048667391588 26.919252823330076 22.968876386374536 60-61 23.287316940813632 26.60567409930245 27.425850367779802 22.68115859210411 62-63 23.218653094499768 26.643398764519688 27.370896370850655 22.76705177012989 64-65 23.029108178382952 26.74453062755434 27.162517769798246 23.063843424264462 66-67 23.096623532962344 26.887164819963942 26.936857962691203 23.07935368438251 68-69 23.041679558684535 26.637272160479274 27.197635797920316 23.123412482915874 70-71 23.151553912887422 26.76474422573556 27.007849985947402 23.07585187542962 72-73 23.278065975443756 26.698188756679148 27.019744550016593 23.004000717860503 74-75 23.099680179600572 27.073880374641917 26.696749649535086 23.129689796222426 76-77 23.212775165842874 26.941179084909223 26.723892908199925 23.122152841047978 78-79 23.300228843026215 26.91009423794764 26.710883124117924 23.078793794908222 80-81 23.207181624044242 27.179439635711777 26.645974052528675 22.967404687715305 82-83 23.235185361177496 27.084475862482073 26.60895796473429 23.071380811606144 84-85 23.205970443670882 27.0602981200451 26.51154846371473 23.222182972569293 86-87 23.24825685537408 26.972172124459565 26.573095028606346 23.206475991560012 88-89 23.191610657768752 27.040945610983353 26.362043463709217 23.405400267538674 90-91 23.170120142210443 27.03116119124002 26.568945498345148 23.22977316820439 92-93 23.119076628671166 26.903600461626404 26.72446943756795 23.25285347213448 94-95 22.982020212709667 26.832784803428755 26.72598550187841 23.459209481983173 96-97 23.070244523777724 26.770431606299976 26.667482246844372 23.491841623077928 98-99 22.952126038382477 26.866590922175078 26.35605639860341 23.825226640839038 100-101 22.618756333939245 26.941857718452646 26.593337606052476 23.846048341555637 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 305.0 1 228.5 2 220.5 3 302.0 4 274.5 5 257.5 6 403.0 7 824.0 8 1309.5 9 1628.5 10 1653.5 11 1561.0 12 1685.0 13 1872.0 14 2072.5 15 2256.5 16 2499.0 17 2841.5 18 3057.5 19 3306.0 20 3650.5 21 4053.0 22 4668.5 23 5634.5 24 6872.0 25 8285.0 26 10415.5 27 13089.5 28 15688.0 29 18892.0 30 22769.0 31 27122.0 32 32451.5 33 36441.0 34 39899.0 35 43939.0 36 48846.5 37 53701.5 38 56223.5 39 57887.0 40 60585.0 41 66042.0 42 70961.0 43 74905.0 44 79033.5 45 82749.0 46 85392.5 47 89271.0 48 99360.0 49 100572.5 50 93408.0 51 89789.0 52 87545.0 53 87147.0 54 86354.0 55 82317.0 56 74831.0 57 67182.5 58 62207.5 59 56361.5 60 47727.0 61 39332.5 62 32458.5 63 25640.0 64 19289.5 65 15258.5 66 11670.0 67 9291.5 68 7038.5 69 4747.5 70 3604.0 71 2757.5 72 2259.5 73 1854.5 74 1427.0 75 1129.0 76 790.5 77 448.5 78 291.5 79 216.0 80 143.0 81 96.0 82 64.0 83 39.0 84 24.5 85 13.0 86 8.0 87 3.5 88 2.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0786531311585321 2 0.01189379727890235 3 0.004952221642816993 4 0.010835630261206411 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004105688028660242 10-11 2.1163340353918777E-4 12-13 2.1163340353918777E-4 14-15 1.0581670176959388E-4 16-17 0.0 18-19 2.1163340353918772E-5 20-21 0.0 22-23 0.0013332904422968827 24-25 0.0 26-27 2.1163340353918772E-5 28-29 0.0 30-31 0.0 32-33 0.004592444856800374 34-35 4.4443014743229424E-4 36-37 0.003068684351318222 38-39 1.2698004212351264E-4 40-41 0.0044231381339690234 42-43 0.011682163875363163 44-45 0.00258192752317809 46-47 0.0016930672283135021 48-49 0.010433526794481956 50-51 6.137368702636445E-4 52-53 0.0025396008424702528 54-55 0.011174243706869112 56-57 0.0037882379233514607 58-59 0.001227473740527289 60-61 0.006645288871130495 62-63 0.00397870798653673 64-65 0.017861859258707445 66-67 0.003259154414503491 68-69 1.2698004212351264E-4 70-71 0.0010158403369881011 72-73 0.0 74-75 0.0 76-77 9.946769966341824E-4 78-79 0.010920283622622087 80-81 0.016105302009332186 82-83 0.01310010767907572 84-85 0.008698132885460615 86-87 0.010602833517313306 88-89 0.028549346137436427 90-91 0.02469761819302321 92-93 0.002518437502116334 94-95 0.008507662822275346 96-97 0.010560506836605468 98-99 0.010497016815543712 100-101 0.01278265757376694 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2362576.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.03274932797704 #Duplication Level Percentage of deduplicated Percentage of total 1 78.86528910578444 44.97904264248642 2 12.358018256421797 14.096235148181362 3 3.680035433082544 6.296476151192104 4 1.6401421045163824 3.7416725403657427 5 0.8711298691483014 2.4841465729624255 6 0.5373057592334384 1.8386414807303444 7 0.3530615753615425 1.4095250627454867 8 0.2571997345756196 1.1735046391418833 9 0.19133618670591687 0.9821185896392612 >10 1.1009906484466472 11.71787250764305 >50 0.09350926975698295 3.6934655750135414 >100 0.04796502130741603 4.977396351009576 >500 0.0029011924203993533 1.1522163109233172 >1k 9.670641401326018E-4 0.884420841648795 >5k 1.4877909848193876E-4 0.5732655863167282 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7087 0.2999691861764447 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6425 0.2719489235478562 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3833 0.16223816715314132 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3474 0.14704288877902763 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 4.2326680707837544E-5 0.0 0.0 0.0 8 0.0 4.2326680707837544E-5 0.0 0.0 0.0 9 0.0 4.2326680707837544E-5 0.0 0.0 4.2326680707837544E-5 10-11 0.0 4.2326680707837544E-5 0.0 0.0 4.2326680707837544E-5 12-13 0.0 4.2326680707837544E-5 0.0 0.0 4.2326680707837544E-5 14-15 0.0 4.2326680707837544E-5 0.0 4.2326680707837544E-5 4.2326680707837544E-5 16-17 0.0 4.2326680707837544E-5 0.0 8.465336141567509E-5 4.2326680707837544E-5 18-19 0.0 4.2326680707837544E-5 0.0 1.0581670176959386E-4 4.2326680707837544E-5 20-21 0.0 4.2326680707837544E-5 0.0 1.2698004212351264E-4 4.2326680707837544E-5 22-23 0.0 4.2326680707837544E-5 0.0 1.9047006318526896E-4 4.2326680707837544E-5 24-25 0.0 4.2326680707837544E-5 0.0 4.232668070783755E-4 4.2326680707837544E-5 26-27 0.0 4.2326680707837544E-5 0.0 5.714101895558069E-4 4.2326680707837544E-5 28-29 0.0 4.2326680707837544E-5 0.0 0.0011004936984037763 4.2326680707837544E-5 30-31 0.0 4.2326680707837544E-5 0.0 0.002327967438931065 4.2326680707837544E-5 32-33 0.0 4.2326680707837544E-5 0.0 0.003915217965474973 4.2326680707837544E-5 34-35 0.0 6.349002106175632E-5 0.0 0.005311998428833612 4.2326680707837544E-5 36-37 0.0 8.465336141567509E-5 0.0 0.008126722695904809 4.2326680707837544E-5 38-39 0.0 8.465336141567509E-5 0.0 0.012168920703503294 4.2326680707837544E-5 40-41 0.0 8.465336141567509E-5 0.0 0.019216313041358245 4.2326680707837544E-5 42-43 0.0 8.465336141567509E-5 0.0 0.025861601912488742 4.2326680707837544E-5 44-45 0.0 1.0581670176959386E-4 0.0 0.034602061478657194 4.2326680707837544E-5 46-47 0.0 1.2698004212351264E-4 0.0 0.04931058302463075 4.2326680707837544E-5 48-49 0.0 1.2698004212351264E-4 0.0 0.059617129776989186 4.2326680707837544E-5 50-51 0.0 1.2698004212351264E-4 0.0 0.07252676739287964 4.2326680707837544E-5 52-53 0.0 1.2698004212351264E-4 0.0 0.08607130521938766 4.2326680707837544E-5 54-55 0.0 1.2698004212351264E-4 0.0 0.1038273477763255 4.2326680707837544E-5 56-57 0.0 1.2698004212351264E-4 0.0 0.14805872911601575 4.2326680707837544E-5 58-59 0.0 1.2698004212351264E-4 0.0 0.1900891230588984 4.2326680707837544E-5 60-61 0.0 1.481433824774314E-4 0.0 0.2210510899966816 4.2326680707837544E-5 62-63 0.0 1.6930672283135018E-4 0.0 0.2576636688089611 4.2326680707837544E-5 64-65 0.0 1.6930672283135018E-4 0.0 0.2959481515092001 4.2326680707837544E-5 66-67 0.0 2.1163340353918774E-4 0.0 0.3627184903258139 4.2326680707837544E-5 68-69 0.0 2.3279674389310652E-4 0.0 0.442927550267166 8.465336141567509E-5 70-71 0.0 2.962867649548628E-4 0.0 0.48218554662368535 8.465336141567509E-5 72-73 0.0 3.3861344566270035E-4 0.0 0.5292951422515085 8.465336141567509E-5 74-75 0.0 3.3861344566270035E-4 0.0 0.614096647049661 8.465336141567509E-5 76-77 0.0 3.3861344566270035E-4 0.0 0.7221566628967703 8.465336141567509E-5 78-79 0.0 3.809401263705379E-4 0.0 0.8252856204414165 8.465336141567509E-5 80-81 0.0 3.809401263705379E-4 0.0 0.9073782176742674 8.465336141567509E-5 82-83 0.0 3.809401263705379E-4 0.0 1.0429294126411173 8.465336141567509E-5 84-85 0.0 4.021034667244567E-4 0.0 1.2647847095712477 8.465336141567509E-5 86-87 0.0 4.4443014743229424E-4 0.0 1.4726510385274378 8.465336141567509E-5 88-89 4.2326680707837544E-5 4.65593487786213E-4 0.0 1.7416794211064532 8.465336141567509E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13365 0.0 26.877756 1 CGAATCG 90 8.9551747E-4 26.386456 3 GTACATG 9280 0.0 25.771622 1 TACCTGG 2095 0.0 24.712893 2 GGTATCA 9615 0.0 24.474125 1 TACATGG 9525 0.0 24.036053 2 GTACCTG 2220 0.0 23.579466 1 ACATGGG 9555 0.0 23.312868 3 TCAACGC 16045 0.0 22.319063 4 ATCAACG 16085 0.0 22.264032 3 CAACGCA 16255 0.0 22.057606 5 TATCAAC 16200 0.0 22.019428 2 GAGTACT 8695 0.0 21.736233 12-13 AACGCAG 16640 0.0 21.632877 6 GCGTATC 140 4.50746E-4 20.581812 1 GTACTTT 9100 0.0 20.534029 14-15 AGTACTT 9100 0.0 19.82956 12-13 ACGCAGA 18410 0.0 19.475632 7 CGCAGAG 18655 0.0 19.194397 8 CATGGGG 4975 0.0 19.093311 4 >>END_MODULE