FastQCFastQC Report
Thu 26 May 2016
SRR936629_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936629_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425696
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67570.27855922588815746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52820.21775193593921086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31810.1311376198831181No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26560.10949434718942522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA123050.028.1577111
GGTATCA88200.026.2428991
GTACCTG21200.025.1092431
TACCTGG21200.024.6613732
GTACATG101050.023.9875161
TACATGG99700.023.7407592
TCAACGC146950.023.2561384
ATCAACG147150.023.2558733
TATCAAC147750.023.0970942
CAACGCA149100.022.9498255
AACGCAG150450.022.9008946
ACATGGG101950.022.2844053
GAGTACT72700.022.06410412-13
ACGCAGA165750.020.5862627
AGTACTT76400.020.46677812-13
CGCAGAG167350.020.389028
GTACTTT78250.020.3471214-15
GTATAGC8800.019.4433571
GCAGAGT174900.019.2088079
AGAGTAC133450.018.99726510-11
Produced by FastQC (version 0.11.5)