##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936629_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2425696 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.2479564628049 31.0 31.0 34.0 28.0 34.0 2 31.28727136458979 31.0 31.0 34.0 28.0 34.0 3 31.33975815600966 31.0 31.0 34.0 28.0 34.0 4 34.96642077160534 37.0 35.0 37.0 32.0 37.0 5 34.92943880849043 37.0 35.0 37.0 32.0 37.0 6 35.04690901085709 37.0 35.0 37.0 32.0 37.0 7 34.8967306702901 37.0 35.0 37.0 32.0 37.0 8 34.83678828674327 37.0 35.0 37.0 32.0 37.0 9 36.30342301755867 39.0 37.0 39.0 32.0 39.0 10-11 36.20200635199134 39.0 35.0 39.0 32.0 39.0 12-13 36.0849263469124 39.0 35.0 39.0 31.0 39.0 14-15 37.139106672888936 39.5 36.5 41.0 31.0 41.0 16-17 37.00822794777251 39.0 36.0 41.0 31.0 41.0 18-19 36.93912180256718 39.0 36.0 41.0 31.0 41.0 20-21 36.836131155759006 39.0 36.0 41.0 30.0 41.0 22-23 36.697598751038875 39.0 36.0 41.0 30.0 41.0 24-25 36.82420468187275 39.0 36.0 41.0 30.0 41.0 26-27 36.809186518013796 39.0 36.0 41.0 30.0 41.0 28-29 36.7037209114415 39.0 36.0 41.0 30.0 41.0 30-31 36.520936052992624 39.0 36.0 41.0 30.0 41.0 32-33 36.35108459592628 39.0 35.0 40.5 30.0 41.0 34-35 36.130860379866235 39.0 35.0 40.0 29.0 41.0 36-37 35.939575280661714 38.0 35.0 40.0 29.0 41.0 38-39 35.737355175586714 38.0 35.0 40.0 27.5 41.0 40-41 35.55252306966743 38.0 35.0 40.0 27.0 41.0 42-43 35.32689524985818 38.0 34.0 40.0 26.5 41.0 44-45 35.072691507921846 38.0 34.0 40.0 25.5 41.0 46-47 34.82183031179505 38.0 34.0 40.0 24.5 41.0 48-49 34.7407867680039 38.0 34.0 40.0 24.0 41.0 50-51 33.82485150653668 37.5 33.0 39.5 23.5 40.5 52-53 33.973703423677165 37.5 33.0 39.5 23.5 40.5 54-55 34.384212407490466 38.0 34.0 40.0 24.0 41.0 56-57 34.29199124704827 38.0 33.5 40.0 23.0 41.0 58-59 34.02543455569041 38.0 33.0 40.0 23.0 41.0 60-61 33.69201148866139 37.0 33.0 40.0 22.0 41.0 62-63 33.35317306867802 37.0 32.5 40.0 21.5 41.0 64-65 32.99550129117581 36.0 32.0 39.0 20.5 41.0 66-67 32.605038306531405 36.0 32.0 39.0 19.5 41.0 68-69 32.18988199675475 35.0 31.0 39.0 18.0 40.0 70-71 31.75288102878514 35.0 31.0 38.0 16.5 40.0 72-73 31.354351081091778 35.0 31.0 37.0 13.5 39.5 74-75 30.926083482843687 35.0 30.0 37.0 11.0 39.0 76-77 30.492184511167103 34.5 30.0 36.0 7.5 39.0 78-79 30.046998675843966 34.0 29.5 36.0 7.0 38.0 80-81 29.573748730261336 34.0 29.0 35.0 3.5 37.0 82-83 29.105706980594434 34.0 29.0 35.0 2.0 37.0 84-85 28.79429759541179 34.0 29.0 35.0 2.0 36.0 86-87 28.463438328628154 34.0 28.5 35.0 2.0 36.0 88-89 28.059946506074958 34.0 27.0 35.0 2.0 35.5 90-91 27.65303257291928 33.0 26.5 35.0 2.0 35.0 92-93 27.280527733071253 33.0 26.0 35.0 2.0 35.0 94-95 26.818464679827976 33.0 25.0 35.0 2.0 35.0 96-97 26.21624123550519 33.0 24.5 35.0 2.0 35.0 98-99 25.485659373639564 32.5 21.5 35.0 2.0 35.0 100-101 23.041740597337835 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3597129326616795 1101 2 0.5244608717061183 1101 3 0.46066504410098474 1101 4 0.514997099580917 1101 5 0.4803831187693035 1101 6 0.2134250033712206 1101 7 0.3069467935673771 1101 8 0.456536486250144 1101 9 0.6404777931687491 1101 10-11 0.5311117570908763 1101 12-13 0.664698520114051 1101 14-15 0.8692383726355857 1101 16-17 0.8856434929400194 1101 18-19 0.8170149076861364 1101 20-21 0.8651818776826943 1101 22-23 0.8007833890375977 1101 24-25 0.7334830311088112 1101 26-27 0.6580853953478467 1101 28-29 0.6966255835010386 1101 30-31 0.6772867065150692 1101 32-33 0.6448950024639473 1101 34-35 0.7059629090922641 1101 36-37 0.5470047884512894 1101 38-39 0.568794976664158 1101 40-41 0.5859184517168785 1101 42-43 0.5041562114307752 1101 44-45 0.49258872878311877 1101 46-47 0.6986957193665617 1101 48-49 0.8456914592503821 1101 50-51 0.7576738673245913 1101 52-53 0.7296127221222619 1101 54-55 0.8954576272674828 1101 56-57 0.676681817930934 1101 58-59 0.5540290268173607 1101 60-61 0.5166617457790537 1101 62-63 0.6451111387742117 1101 64-65 0.7297999845093273 1101 66-67 0.7425739379624687 1101 68-69 0.7324453099539951 1101 70-71 0.864917682274875 1101 72-73 0.7387725435129369 1101 74-75 0.5874580258261801 1101 76-77 0.7320851854712167 1101 78-79 0.5151772017687897 1101 80-81 0.6239945160603249 1101 82-83 0.5773792042182286 1101 84-85 0.6933166009818947 1101 86-87 0.564442000175216 1101 88-89 0.6444450886178608 1101 90-91 0.5005667753006122 1101 92-93 0.6325315835155862 1101 94-95 0.5952372153579226 1101 96-97 0.46179159233875566 1101 98-99 0.5187622784443242 1101 100-101 0.8746987425253998 1102 1 0.25215499884302517 1102 2 0.2115901967305902 1102 3 0.22693941990604216 1102 4 0.18588211550215306 1102 5 0.2664565829255281 1102 6 0.1831321855343404 1102 7 0.22827704297622375 1102 8 0.01068688656079786 1102 9 0.3428770378787789 1102 10-11 0.2677479942128116 1102 12-13 0.22057644918450592 1102 14-15 0.31952763172836995 1102 16-17 0.21314658941983566 1102 18-19 0.4649752198800243 1102 20-21 0.30552161158907865 1102 22-23 0.1213994386370203 1102 24-25 0.11225162635765429 1102 26-27 0.10921111549190954 1102 28-29 0.18892676570813194 1102 30-31 0.13588212477022665 1102 32-33 0.02843209852636619 1102 34-35 -0.17949232840705065 1102 36-37 0.008225651534438327 1102 38-39 -0.05231886457956847 1102 40-41 -0.0040099420748518355 1102 42-43 0.09115951462290894 1102 44-45 -0.16378791706092954 1102 46-47 0.05274774359149603 1102 48-49 0.16086480505013157 1102 50-51 0.2856415469117053 1102 52-53 0.21664180878777017 1102 54-55 0.32017204766617624 1102 56-57 0.3756836755184594 1102 58-59 0.44583665855727617 1102 60-61 0.31204819811369333 1102 62-63 0.3101400863099002 1102 64-65 0.35704767321797704 1102 66-67 0.27729482105003456 1102 68-69 0.13903464542401878 1102 70-71 -0.011575815808367196 1102 72-73 -0.11240593302981239 1102 74-75 -0.2667647268034976 1102 76-77 0.0014689568532695318 1102 78-79 -0.15921904023590017 1102 80-81 -0.08502823383001257 1102 82-83 -6.980431160492628E-4 1102 84-85 -0.008735119512277123 1102 86-87 -0.07697987067409429 1102 88-89 -0.17506759412370698 1102 90-91 -0.06416019561824982 1102 92-93 -0.10707600067027911 1102 94-95 -0.004358058601095394 1102 96-97 -0.1904514677197966 1102 98-99 0.02839852121314479 1102 100-101 0.0903558107954936 1103 1 0.2895136538353391 1103 2 0.3821688720086627 1103 3 0.2796127220424687 1103 4 0.35999830210357686 1103 5 0.43712544663221564 1103 6 0.2896326463163206 1103 7 0.25534624865039746 1103 8 0.33546157546202693 1103 9 0.5703465014894391 1103 10-11 0.5122669946932135 1103 12-13 0.3904851101592044 1103 14-15 0.3037123603282126 1103 16-17 0.5090601360236491 1103 18-19 0.4221561807284715 1103 20-21 0.481239337020007 1103 22-23 0.5304465212224514 1103 24-25 0.47071483243676937 1103 26-27 0.3402480325013002 1103 28-29 0.04749917584840091 1103 30-31 0.1753431239493537 1103 32-33 0.4782861248722554 1103 34-35 0.3381605171344617 1103 36-37 0.29893966016378926 1103 38-39 0.4202915012461901 1103 40-41 0.443918796751646 1103 42-43 0.379356879744428 1103 44-45 0.4841527985957157 1103 46-47 0.4268695081117926 1103 48-49 0.3312365419524923 1103 50-51 0.45397698122090446 1103 52-53 0.517670439810729 1103 54-55 0.5613067484911198 1103 56-57 0.595277407434736 1103 58-59 0.5189043171437291 1103 60-61 0.5760893109978511 1103 62-63 0.5576809424528335 1103 64-65 0.6208280606515331 1103 66-67 0.5624904327491294 1103 68-69 0.6341070008113405 1103 70-71 0.5729324320864215 1103 72-73 0.5764740510429327 1103 74-75 0.42723994005529775 1103 76-77 0.405775774265404 1103 78-79 0.329170316370476 1103 80-81 0.1457775979686886 1103 82-83 0.21450120630373348 1103 84-85 0.1760597633870411 1103 86-87 0.16594978512846836 1103 88-89 0.4388893489304486 1103 90-91 0.46929651194389876 1103 92-93 0.25141008453497093 1103 94-95 0.31347000844838746 1103 96-97 0.520845653168557 1103 98-99 0.6670707157342513 1103 100-101 1.0913521092064293 1104 1 0.16131511420816125 1104 2 0.2423411796259245 1104 3 0.20246625882573 1104 4 0.29418919580889025 1104 5 0.3271114491425351 1104 6 0.20662500649167725 1104 7 0.29067967547452866 1104 8 0.32358766261926775 1104 9 0.46593933916471997 1104 10-11 0.4373238077775099 1104 12-13 0.3001224732655885 1104 14-15 0.4118087801239838 1104 16-17 0.3443927789186816 1104 18-19 0.5014101066889793 1104 20-21 0.5510965661093579 1104 22-23 0.19430947996252712 1104 24-25 0.3702743513830242 1104 26-27 0.08226001268008076 1104 28-29 0.32033611946560114 1104 30-31 0.3068476668185127 1104 32-33 0.0570766616285141 1104 34-35 0.11882673492912943 1104 36-37 -0.03869250810571856 1104 38-39 0.047125133198441915 1104 40-41 0.1309041799579731 1104 42-43 0.30251658439477325 1104 44-45 0.2919259782905641 1104 46-47 0.26613703959743873 1104 48-49 0.3325378642666905 1104 50-51 0.22581746384250323 1104 52-53 0.24245427136003883 1104 54-55 0.40191654081087336 1104 56-57 0.31871810711758997 1104 58-59 0.35334850705316256 1104 60-61 0.4014873945524968 1104 62-63 0.7263141097964336 1104 64-65 0.500397387421927 1104 66-67 0.5062378577597784 1104 68-69 0.5070399321922849 1104 70-71 0.5044727743847055 1104 72-73 0.3736842215649361 1104 74-75 0.12236294288702254 1104 76-77 0.4096344475263649 1104 78-79 0.36708722511154335 1104 80-81 0.3706367738153098 1104 82-83 0.40598677808855754 1104 84-85 0.48418527566293434 1104 86-87 0.5227903940497569 1104 88-89 0.5434367474617154 1104 90-91 0.5689195291476601 1104 92-93 0.46909935768461253 1104 94-95 0.4261964006411496 1104 96-97 0.5577512933642588 1104 98-99 0.7727826845025163 1104 100-101 1.1651842943739688 1105 1 0.3500187926805687 1105 2 0.28806083088651846 1105 3 0.23809952716490201 1105 4 0.33485127889915134 1105 5 0.3940388574425384 1105 6 0.24045511889248417 1105 7 0.36081849777404784 1105 8 0.41665192901731984 1105 9 0.540718013676937 1105 10-11 0.5159414594488325 1105 12-13 0.4161967293664901 1105 14-15 0.18095436963304934 1105 16-17 0.2956282988352399 1105 18-19 0.3521718810492942 1105 20-21 0.3988985188885934 1105 22-23 0.2149024018467358 1105 24-25 0.3619343429917805 1105 26-27 0.4188503741910168 1105 28-29 0.24827292841904836 1105 30-31 0.1895480929890354 1105 32-33 0.24585723571295404 1105 34-35 0.11649309159524535 1105 36-37 0.18049922536538787 1105 38-39 0.13070011176197482 1105 40-41 0.010868942510782631 1105 42-43 0.35506101799001044 1105 44-45 0.01605189083242209 1105 46-47 0.29884471347615715 1105 48-49 0.2134214172713058 1105 50-51 0.31320312778335335 1105 52-53 0.10161323120905763 1105 54-55 0.1778763609410916 1105 56-57 0.4514555632076309 1105 58-59 0.4968301135040605 1105 60-61 0.30208082291895977 1105 62-63 0.4995294292489021 1105 64-65 0.5802615921452201 1105 66-67 0.6350498126401192 1105 68-69 0.5945732541737314 1105 70-71 0.684855701342979 1105 72-73 0.5388660862822086 1105 74-75 0.6896167288747925 1105 76-77 0.40827927777369766 1105 78-79 0.5251037509414154 1105 80-81 0.433780139814548 1105 82-83 0.4889498232181033 1105 84-85 0.6605109368893558 1105 86-87 0.6904946458758943 1105 88-89 0.5272115095304706 1105 90-91 0.6298916163042847 1105 92-93 0.7895223837920575 1105 94-95 0.7259000821471737 1105 96-97 0.9330359093484937 1105 98-99 1.0557285303390778 1105 100-101 1.2775662421332257 1106 1 0.16054894058840574 1106 2 0.15230282467841505 1106 3 0.2511710275162464 1106 4 0.2690229833252573 1106 5 0.18687391681380205 1106 6 0.2647690711907771 1106 7 0.3026719582185038 1106 8 0.33064145670547873 1106 9 0.5802861252607059 1106 10-11 0.5521198418111624 1106 12-13 0.598854770537244 1106 14-15 0.5733895221464849 1106 16-17 0.7410958264945435 1106 18-19 0.621004904481147 1106 20-21 0.4299472131466828 1106 22-23 0.47326283868076047 1106 24-25 0.4034386576859177 1106 26-27 0.16102090266291924 1106 28-29 0.42692771098773363 1106 30-31 0.42725397942290044 1106 32-33 0.36010249222826474 1106 34-35 0.40216430936040126 1106 36-37 0.3843940241721171 1106 38-39 0.3683679445374395 1106 40-41 0.22800188097444618 1106 42-43 0.09268818692833491 1106 44-45 0.2848050977461014 1106 46-47 0.22407614359995875 1106 48-49 0.2853955160555799 1106 50-51 0.18687817014724573 1106 52-53 0.4803437776499706 1106 54-55 0.623746474325884 1106 56-57 0.46247374585007606 1106 58-59 0.3598521921817124 1106 60-61 0.4167858830251916 1106 62-63 0.5671651362192449 1106 64-65 0.4383988586973544 1106 66-67 0.5446043719011442 1106 68-69 0.5726851882543542 1106 70-71 0.624509880136106 1106 72-73 0.7046027508446997 1106 74-75 0.39723281829127544 1106 76-77 0.6310054171338884 1106 78-79 0.7263125384515732 1106 80-81 0.5858663251688405 1106 82-83 0.7671913732796156 1106 84-85 0.6859762527656308 1106 86-87 0.7351352276984713 1106 88-89 0.8510947823374195 1106 90-91 0.8421816428800248 1106 92-93 0.9102825777420769 1106 94-95 0.9126421600829921 1106 96-97 0.9519337407707873 1106 98-99 1.389779617032044 1106 100-101 1.7348552005963533 1107 1 0.18711865904452196 1107 2 0.3212547157033647 1107 3 0.11347896079813324 1107 4 0.25097397344868 1107 5 0.3384238911481816 1107 6 0.34589521893724395 1107 7 0.3677355249133498 1107 8 0.3904478427567142 1107 9 0.504095139693888 1107 10-11 0.3985710746987223 1107 12-13 0.3120473863399127 1107 14-15 0.21804298068452255 1107 16-17 0.19404647001051956 1107 18-19 0.42144368589548264 1107 20-21 0.4576335077046636 1107 22-23 0.5188735311385386 1107 24-25 0.42512065542289434 1107 26-27 0.2819158444863561 1107 28-29 0.42531268697873514 1107 30-31 0.3224896203931493 1107 32-33 0.23498296822656783 1107 34-35 0.47307102472344553 1107 36-37 0.5120164151522673 1107 38-39 0.3182980326340825 1107 40-41 0.14765903827935034 1107 42-43 0.3629330140378997 1107 44-45 0.2970138762917216 1107 46-47 0.38045965953313043 1107 48-49 0.44230609758508166 1107 50-51 0.22607141526122376 1107 52-53 0.3460513118123387 1107 54-55 0.4952106094847295 1107 56-57 0.5006768328096456 1107 58-59 0.5764716373458469 1107 60-61 0.6254243964612627 1107 62-63 0.6363288120363961 1107 64-65 0.6346980080157039 1107 66-67 0.5080365913904785 1107 68-69 0.5726931762015894 1107 70-71 0.6904116950671728 1107 72-73 0.627335406753808 1107 74-75 0.6960288609927581 1107 76-77 0.7215457427226148 1107 78-79 0.6607414939962162 1107 80-81 0.8487413332436127 1107 82-83 0.9637925364636537 1107 84-85 0.9436053327833314 1107 86-87 0.8606675549209122 1107 88-89 0.8728974328077719 1107 90-91 0.835473086955016 1107 92-93 0.8203137029521095 1107 94-95 0.912477955672113 1107 96-97 0.8248922472052556 1107 98-99 1.13223111109075 1107 100-101 1.498819973054463 1108 1 0.011322649029512633 1108 2 0.29132232361869725 1108 3 0.22806369541143212 1108 4 0.26197553203437707 1108 5 0.30449817555030023 1108 6 0.10075455976953407 1108 7 0.013462585992485288 1108 8 0.18338459217622471 1108 9 0.2554897098792708 1108 10-11 -0.0590539386448512 1108 12-13 0.20253552483085713 1108 14-15 0.2533040357226142 1108 16-17 0.3151811641617499 1108 18-19 0.21617587385325265 1108 20-21 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-3.699546706158081 2316 90-91 -3.6036032034058287 2316 92-93 -3.8875540647748252 2316 94-95 -4.203155346981649 2316 96-97 -5.0336456231538556 2316 98-99 -6.521763289994823 2316 100-101 -6.962558331525969 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6666.0 3 2777.0 4 2777.0 5 3012.0 6 3422.0 7 3877.0 8 4694.0 9 6091.0 10 7297.0 11 8798.0 12 10683.0 13 12634.0 14 14026.0 15 16105.0 16 18680.0 17 22530.0 18 20074.0 19 15960.0 20 17082.0 21 18410.0 22 20370.0 23 23089.0 24 26532.0 25 30014.0 26 35331.0 27 41476.0 28 48918.0 29 58859.0 30 70703.0 31 86576.0 32 107084.0 33 136257.0 34 178044.0 35 240727.0 36 332649.0 37 421015.0 38 310051.0 39 42398.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.11319838110108 17.63426971806184 13.875121759707165 23.377410141129914 2 15.89451434688342 19.14020137510381 40.12125555538145 24.84402872263132 3 19.914068056944345 23.371962848041893 28.807941694929234 27.906027400084533 4 13.000607146510394 15.555737039566814 36.266609911666784 35.17704590225601 5 14.289744837770279 36.55308248842984 33.65456165389343 15.502611019906448 6 33.350628931466126 34.53717845551408 17.31843437166041 14.793758241359384 7 30.183188015091268 31.53487658432906 19.76477954372313 18.517155856856547 8 27.1518311521677 33.95400765727922 19.199248047426792 19.694913143126286 9 26.71834433494388 16.631548123539737 19.888189817478434 36.76191772403795 10-11 25.71296817989459 25.821817968835713 26.73394537794676 21.731268473322935 12-13 26.678338803358525 22.950637791532753 27.299395461683527 23.071627943425195 14-15 23.194413999726564 24.59287306968746 25.653971442705696 26.558741487880276 16-17 22.940474940849445 27.913431594160276 26.216569084320756 22.92952438066952 18-19 22.771225843573703 26.418326763067235 28.46078412164496 22.3496632717141 20-21 23.49320681163887 25.531585929374756 28.450229497517427 22.524977761468946 22-23 23.072734657356037 25.54091749701569 28.391566004242975 22.994781841385294 24-25 23.035769943056355 25.630185409036116 28.562069892428788 22.771974755478745 26-27 22.8970838432933 25.852223239908874 28.49679985305732 22.753893063740513 28-29 22.50641041630034 26.10828207851886 28.653133347996707 22.732174157184087 30-31 22.698835982500775 25.687915665318066 28.593073780478 23.020174571703155 32-33 22.557881340314946 25.83058522622566 28.532133579649482 23.07939985380991 34-35 23.243361915157436 25.790679624859692 28.161311568681413 22.80464689130146 36-37 23.19940349803791 25.593801459013783 28.262290175033826 22.94450486791448 38-39 22.86905771301383 25.823881891141898 28.40242126787342 22.904639127970846 40-41 23.049599705151135 25.750154752366328 28.39521950718766 22.805026035294876 42-43 22.962455576276263 25.800379404006314 28.0512638589341 23.18590116078332 44-45 22.96307839643937 25.961359880559332 27.93824061654669 23.137321106454607 46-47 23.177005767233318 26.167705513470104 27.551313823942213 23.10397489535437 48-49 23.29598977604172 26.19054489987323 27.414327970564894 23.099137353520156 50-51 23.07807421552283 26.39708586250851 27.413228884788342 23.111611037180317 52-53 23.34003472520686 26.44541148421916 27.167496249920898 23.047057540653086 54-55 22.992556281397714 26.17748249355297 27.347966531095203 23.481994693954118 56-57 23.08437495490258 26.61718835362975 27.138566077256264 23.159870614211407 58-59 23.323791437773867 26.18316481435976 27.180507981219108 23.31253576664726 60-61 23.1677264730608 26.183732208920183 27.68737504859937 22.961166269419646 62-63 23.05049932860782 26.306411269658884 27.569007860874844 23.07408154085845 64-65 22.877402146601284 26.427518711132276 27.392438043529793 23.30264109873665 66-67 22.86090758663965 26.59293129041787 27.116695158832922 23.429465964109557 68-69 22.883663223450593 26.356931156020075 27.43958153626922 23.31982408426011 70-71 22.96675395319757 26.381771620422946 27.259701101179058 23.391773325200425 72-73 23.034594922175337 26.299108072285925 27.330771815331595 23.33552519020714 74-75 22.800374997217205 26.707853421272436 27.087632924887593 23.404138656622766 76-77 23.055373522517595 26.541303336395888 26.98341171118225 23.419911429904268 78-79 23.097405241052332 26.5345461104383 27.03669767322013 23.331350975289237 80-81 23.04810987708403 26.788033086814234 26.965341304403843 23.19851573169789 82-83 23.093417349999516 26.688827958848933 26.953778121708865 23.26397656944269 84-85 23.038239145103663 26.615336436896992 26.849845780470805 23.496578637528547 86-87 23.09986122941013 26.556428013684112 27.017574309878107 23.326136447027647 88-89 23.04015574241402 26.536815357052966 26.804157501195093 23.61887139933792 90-91 22.955013333833556 26.535410980350605 27.089954986330167 23.419620699485673 92-93 23.031753276820574 26.382626758911194 27.232585918484904 23.353034045783332 94-95 22.850038364378726 26.394754408192867 27.141942429106297 23.61326479832211 96-97 22.850491988742764 26.350876657725415 27.117885169918143 23.680746183613678 98-99 22.7967310245268 26.401683845233524 26.898165835004527 23.903419295235146 100-101 22.73047918985166 26.210821099802228 27.122569970477862 23.936129739868246 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 187.0 1 146.0 2 191.0 3 285.0 4 259.5 5 237.0 6 398.5 7 785.0 8 1332.0 9 1671.0 10 1579.5 11 1485.0 12 1606.5 13 1746.0 14 1916.0 15 2095.0 16 2236.5 17 2435.0 18 2608.5 19 2838.0 20 3227.5 21 3752.5 22 4327.0 23 5221.0 24 6515.5 25 8114.5 26 10265.0 27 12787.0 28 15480.0 29 18838.0 30 22808.5 31 27361.0 32 32573.5 33 36818.0 34 40911.0 35 45045.5 36 49535.0 37 54208.5 38 57330.5 39 59312.5 40 62089.5 41 67210.5 42 72298.5 43 76929.0 44 81202.5 45 85672.0 46 88726.5 47 91974.5 48 101847.0 49 103810.5 50 96402.0 51 92505.5 52 90893.5 53 90922.5 54 90013.0 55 85379.0 56 77951.0 57 70086.5 58 64050.0 59 57651.5 60 49131.0 61 41022.5 62 33960.0 63 27035.0 64 20517.5 65 15833.5 66 12371.5 67 9954.0 68 7452.0 69 5057.0 70 3809.0 71 2903.5 72 2241.0 73 1821.0 74 1378.0 75 1069.5 76 784.0 77 474.5 78 292.5 79 175.5 80 117.0 81 90.5 82 59.5 83 39.5 84 29.0 85 22.5 86 17.0 87 12.0 88 7.5 89 6.0 90 7.5 91 4.5 92 1.5 93 1.0 94 2.5 95 2.0 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03454678574726594 2 0.02349841035315225 3 0.02147837156840758 4 0.05111934883843647 5 0.055901481471709556 6 0.048934408928406524 7 0.05140792580768572 8 0.04431717742041871 9 0.04332777066870704 10-11 0.05169650277693495 12-13 0.046110477157896124 14-15 0.04087486643008852 16-17 0.047924389536034194 18-19 0.052088142949487486 20-21 0.011893493661200745 22-23 0.021066118755194384 24-25 0.005771539384984763 26-27 0.01073918578420379 28-29 0.006245630120179939 30-31 0.008945886046726383 32-33 0.003586599474954817 34-35 0.008389344748888566 36-37 0.009152012453332982 38-39 0.011233889160059629 40-41 0.001916975581441368 42-43 0.002040651425405327 44-45 0.002329228394654565 46-47 3.9164017255253754E-4 48-49 8.657309077477146E-4 50-51 1.6490112528527896E-4 52-53 0.003318635146366239 54-55 0.006637270292732478 56-57 0.016119084996636016 58-59 0.010388770892972573 60-61 0.010429996174293894 62-63 0.0057303141036634435 64-65 4.122528132131974E-5 66-67 0.007235036871891614 68-69 0.018304024906665963 70-71 0.006575432370750498 72-73 0.0021024893473873068 74-75 0.0032155719430629393 76-77 0.006575432370750498 78-79 0.005503575056396185 80-81 0.010656735221561152 82-83 0.005998278432252022 84-85 0.01729400551429363 86-87 0.0045347809453451715 88-89 0.00628685540150126 90-91 0.0041019154914713135 92-93 0.016613788372491854 94-95 0.01108960067543501 96-97 0.009646715829188818 98-99 0.02195246230360276 100-101 0.03732949223645502 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2425696.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.92937545968474 #Duplication Level Percentage of deduplicated Percentage of total 1 80.26953014548643 47.30233279915853 2 11.543021561481357 13.604461030715425 3 3.484251469813709 6.159742891818313 4 1.4796866544925424 3.4878804170110334 5 0.7964487346976407 2.3467113260694052 6 0.505416013009212 1.7870309996374054 7 0.33301768987924196 1.3737167137127493 8 0.23533795713791641 1.1094655068876376 9 0.18905232732694732 1.0026662020719208 >10 1.0302303795485468 11.342688493604458 >50 0.08433678651686867 3.4047808620256417 >100 0.04609271439341685 4.80146098329657 >500 0.0027357859299700117 1.084378121693539 >1k 7.01483571786026E-4 0.6950156053678821 >5k 1.402967143572052E-4 0.49766804692939587 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6757 0.27855922588815746 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5282 0.21775193593921086 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3181 0.1311376198831181 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2656 0.10949434718942522 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.1225281321319734E-5 0.0 3 0.0 0.0 0.0 4.1225281321319734E-5 0.0 4 0.0 0.0 0.0 4.1225281321319734E-5 0.0 5 0.0 0.0 0.0 4.1225281321319734E-5 0.0 6 0.0 0.0 0.0 4.1225281321319734E-5 0.0 7 0.0 4.1225281321319734E-5 0.0 4.1225281321319734E-5 0.0 8 0.0 4.1225281321319734E-5 0.0 4.1225281321319734E-5 0.0 9 0.0 4.1225281321319734E-5 0.0 4.1225281321319734E-5 4.1225281321319734E-5 10-11 0.0 4.1225281321319734E-5 0.0 1.2367584396395922E-4 4.1225281321319734E-5 12-13 0.0 4.1225281321319734E-5 0.0 1.2367584396395922E-4 4.1225281321319734E-5 14-15 0.0 8.245056264263947E-5 0.0 1.2367584396395922E-4 4.1225281321319734E-5 16-17 0.0 8.245056264263947E-5 0.0 1.2367584396395922E-4 4.1225281321319734E-5 18-19 0.0 8.245056264263947E-5 0.0 1.2367584396395922E-4 4.1225281321319734E-5 20-21 0.0 8.245056264263947E-5 0.0 2.4735168792791844E-4 4.1225281321319734E-5 22-23 0.0 8.245056264263947E-5 0.0 4.1225281321319735E-4 4.1225281321319734E-5 24-25 0.0 1.0306320330329935E-4 0.0 5.359286571771566E-4 4.1225281321319734E-5 26-27 0.0 1.2367584396395922E-4 0.0 8.657309077477146E-4 8.245056264263947E-5 28-29 0.0 1.2367584396395922E-4 0.0 0.0012985963616215716 8.245056264263947E-5 30-31 0.0 1.2367584396395922E-4 0.0 0.0024735168792791844 8.245056264263947E-5 32-33 0.0 1.2367584396395922E-4 0.0 0.004328654538738572 8.245056264263947E-5 34-35 0.0 1.2367584396395922E-4 0.0 0.005998278432252022 8.245056264263947E-5 36-37 0.0 1.442884846246191E-4 0.0 0.008739759640119783 8.245056264263947E-5 38-39 0.0 1.6490112528527894E-4 0.0 0.012882900412912419 8.245056264263947E-5 40-41 0.0 1.6490112528527894E-4 0.0 0.020447739535374588 8.245056264263947E-5 42-43 0.0 2.0612640660659868E-4 0.0 0.027270523594053006 8.245056264263947E-5 44-45 0.0 2.2673904726725857E-4 0.0 0.03592783267153015 8.245056264263947E-5 46-47 0.0 2.8857696924923813E-4 0.0 0.052521008403361345 8.245056264263947E-5 48-49 0.0 2.8857696924923813E-4 0.0 0.0635899964381357 8.245056264263947E-5 50-51 0.0 2.8857696924923813E-4 0.0 0.07721495191483187 8.245056264263947E-5 52-53 0.0 2.8857696924923813E-4 0.0 0.09083990739152804 1.0306320330329935E-4 54-55 0.0 2.8857696924923813E-4 0.0 0.10928822078281862 1.2367584396395922E-4 56-57 0.0 2.8857696924923813E-4 0.0 0.15422377742305715 1.2367584396395922E-4 58-59 0.0 2.8857696924923813E-4 0.0 0.1969331688719444 1.2367584396395922E-4 60-61 0.0 3.2980225057055787E-4 0.0 0.23022258353891006 1.6490112528527894E-4 62-63 0.0 3.2980225057055787E-4 0.0 0.2682735181984882 1.6490112528527894E-4 64-65 0.0 3.2980225057055787E-4 0.0 0.30943696159782597 1.6490112528527894E-4 66-67 0.0 3.2980225057055787E-4 0.0 0.37768541482527074 1.6490112528527894E-4 68-69 0.0 3.2980225057055787E-4 0.0 0.45813655132382625 1.6490112528527894E-4 70-71 0.0 3.710275318918776E-4 0.0 0.4997328601770379 1.6490112528527894E-4 72-73 0.0 4.1225281321319735E-4 0.0 0.5464204912734325 1.6490112528527894E-4 74-75 0.0 4.1225281321319735E-4 0.0 0.6331172578921679 1.6490112528527894E-4 76-77 0.0 4.1225281321319735E-4 0.0 0.739952574436368 1.6490112528527894E-4 78-79 0.0 4.1225281321319735E-4 0.0 0.8454686819782857 1.6490112528527894E-4 80-81 0.0 4.1225281321319735E-4 0.0 0.9317119705024867 1.6490112528527894E-4 82-83 0.0 4.1225281321319735E-4 0.0 1.0636740960120312 1.6490112528527894E-4 84-85 0.0 4.1225281321319735E-4 0.0 1.283198719048059 1.6490112528527894E-4 86-87 0.0 4.328654538738572E-4 0.0 1.4913451644394022 1.6490112528527894E-4 88-89 0.0 4.534780945345171E-4 0.0 1.7566092371014341 1.6490112528527894E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12305 0.0 28.157711 1 GGTATCA 8820 0.0 26.242899 1 GTACCTG 2120 0.0 25.109243 1 TACCTGG 2120 0.0 24.661373 2 GTACATG 10105 0.0 23.987516 1 TACATGG 9970 0.0 23.740759 2 TCAACGC 14695 0.0 23.256138 4 ATCAACG 14715 0.0 23.255873 3 TATCAAC 14775 0.0 23.097094 2 CAACGCA 14910 0.0 22.949825 5 AACGCAG 15045 0.0 22.900894 6 ACATGGG 10195 0.0 22.284405 3 GAGTACT 7270 0.0 22.064104 12-13 ACGCAGA 16575 0.0 20.586262 7 AGTACTT 7640 0.0 20.466778 12-13 CGCAGAG 16735 0.0 20.38902 8 GTACTTT 7825 0.0 20.34712 14-15 GTATAGC 880 0.0 19.443357 1 GCAGAGT 17490 0.0 19.208807 9 AGAGTAC 13345 0.0 18.997265 10-11 >>END_MODULE