FastQCFastQC Report
Thu 26 May 2016
SRR936629_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936629_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425696
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75780.31240518185296096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63370.2612446077332032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38740.15970673983879266No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36340.14981267232167592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG97850.024.7712421
GTATCAA143950.024.4319671
GTACCTG22600.024.394281
TACCTGG22350.024.0173132
TACATGG100550.022.9603062
GGTATCA108200.022.928821
ACATGGG97900.022.7088763
GAGTACT84450.021.65430612-13
CGCGAAA550.002877538821.59239430-31
TCAACGC169150.020.668194
ATCAACG169600.020.615053
GTACTTT89550.020.42085314-15
CAACGCA172000.020.4085715
TATCAAC172050.020.3762932
AACGCAG176050.019.9116866
AGTACTT88100.019.6788712-13
CATGGGG51950.018.9269834
ACGCAGA192550.018.2297067
ATAGGAC6800.018.163393
AGAGTAC150050.018.12287910-11
Produced by FastQC (version 0.11.5)