FastQCFastQC Report
Thu 26 May 2016
SRR936628_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936628_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2505616
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67270.26847689350642717No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54070.21579523757830407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29970.1196113051640794No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27700.11055165675825825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG101550.027.6153131
GTATCAA127950.027.043881
TACCTGG26900.026.8571872
TACATGG103550.026.5305942
GTACCTG28550.026.1379451
ACATGGG101900.026.0304283
GGTATCA91300.024.3642441
TCAACGC148250.023.1529964
ATCAACG149000.023.1298623
TATCAAC150600.022.8813822
CAACGCA150450.022.8148885
GAGTACT75000.022.27802512-13
AACGCAG154300.022.2456266
CATGGGG54400.022.1972664
CGTACAC3059.094947E-1221.8197363
GTACTTT80800.020.67307714-15
CATGGGA53000.020.3617294
ACGCAGA169700.020.1988567
CGCAGAG171900.019.9707988
TAACGCA2702.2795575E-819.3683934
Produced by FastQC (version 0.11.5)