FastQCFastQC Report
Thu 26 May 2016
SRR936628_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936628_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2505616
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75910.3029594319321077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66490.2653638865652199No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39450.1574463126033678No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36210.14451536069373758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG98400.025.8806461
GGTATCA106350.025.822351
TACCTGG22250.025.620482
GTATCAA145000.025.3617021
TACATGG99200.024.7579632
GTACCTG24400.023.7561151
ACATGGG99850.023.4031893
GAGTACT93150.021.84523212-13
TCAACGC174400.021.0221354
ATCAACG175400.020.9047853
CAACGCA176700.020.8022565
TATCAAC176800.020.7698442
GTACTTT96650.020.7593414-15
AACGCAG179300.020.5519396
AGTACTT97900.020.15473412-13
CATGGGG52050.019.16044
AGAGTAC156100.018.9220210-11
ACGCAGA199400.018.4802537
GTGTAGC12700.018.331491
CGCAGAG201600.018.2314748
Produced by FastQC (version 0.11.5)