##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936628_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2505616 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.876756055197603 31.0 31.0 34.0 30.0 34.0 2 32.03945297284181 31.0 31.0 34.0 30.0 34.0 3 32.124549811303886 33.0 31.0 34.0 30.0 34.0 4 35.82129224909164 37.0 35.0 37.0 35.0 37.0 5 35.64655996768858 37.0 35.0 37.0 33.0 37.0 6 35.73199644319002 37.0 35.0 37.0 33.0 37.0 7 35.67354654504122 37.0 35.0 37.0 33.0 37.0 8 35.641833385482855 37.0 35.0 37.0 33.0 37.0 9 37.27289935888021 39.0 37.0 39.0 34.0 39.0 10-11 37.16305830582181 39.0 37.0 39.0 33.0 39.0 12-13 37.05049077751738 39.0 37.0 39.0 33.0 39.0 14-15 38.19849689657154 40.0 37.5 41.0 33.0 41.0 16-17 38.09234894732472 40.0 37.0 41.0 33.0 41.0 18-19 38.21804777747269 40.0 37.5 41.0 33.5 41.0 20-21 38.22777073581905 40.0 38.0 41.0 33.0 41.0 22-23 38.14222031628151 40.0 37.0 41.0 33.0 41.0 24-25 38.006814092821884 40.0 37.0 41.0 33.0 41.0 26-27 37.81368054801693 40.0 37.0 41.0 33.0 41.0 28-29 37.73072170675794 40.0 37.0 41.0 32.5 41.0 30-31 37.72032785550539 40.0 37.0 41.0 33.0 41.0 32-33 37.58518843270477 40.0 36.5 41.0 32.0 41.0 34-35 37.42350324231646 40.0 36.0 41.0 32.0 41.0 36-37 37.22580994054955 39.0 36.0 41.0 31.0 41.0 38-39 37.064300754784455 39.0 36.0 41.0 31.0 41.0 40-41 36.88187874758144 39.0 35.5 41.0 31.0 41.0 42-43 36.61589684931769 39.0 35.0 40.5 30.0 41.0 44-45 36.360525515482024 39.0 35.0 40.0 30.0 41.0 46-47 36.01176995996194 39.0 35.0 40.0 29.5 41.0 48-49 35.65443767121538 38.0 35.0 40.0 28.0 41.0 50-51 35.36203173191742 38.0 34.5 40.0 27.0 41.0 52-53 34.99038240496549 38.0 34.0 40.0 26.0 41.0 54-55 34.78954656260177 38.0 34.0 40.0 26.0 41.0 56-57 34.48026593061347 38.0 33.5 40.0 26.0 41.0 58-59 34.100115700091315 37.0 33.0 40.0 25.0 41.0 60-61 33.676909390744626 37.0 33.0 39.5 23.5 41.0 62-63 33.33712727728431 36.0 32.0 39.0 23.0 40.0 64-65 32.90202529038767 35.5 31.5 39.0 22.0 40.0 66-67 32.471305658967694 35.0 31.0 38.5 22.0 40.0 68-69 32.10912546056539 35.0 31.0 38.0 21.0 40.0 70-71 31.738614975319443 35.0 31.0 37.5 20.0 39.5 72-73 31.279467204870976 34.5 30.0 37.0 18.5 39.0 74-75 30.774069330655614 34.0 30.0 36.0 17.5 39.0 76-77 29.288487342034855 32.5 28.0 35.0 14.0 37.0 78-79 30.06102690915128 34.0 29.0 35.0 14.5 37.0 80-81 29.84512930153703 34.0 29.0 35.0 10.0 37.0 82-83 29.564909986206985 34.0 29.0 35.0 7.5 36.5 84-85 29.20090748143371 34.0 29.0 35.0 7.0 36.0 86-87 28.806372564670724 33.5 29.0 35.0 2.0 35.5 88-89 28.35062755027107 33.0 28.0 35.0 2.0 35.0 90-91 27.88248239155561 33.0 27.0 35.0 2.0 35.0 92-93 27.26041999252878 33.0 26.0 34.5 2.0 35.0 94-95 26.71376639516989 32.0 25.0 34.0 2.0 35.0 96-97 25.98517749727013 32.0 24.5 34.0 2.0 35.0 98-99 25.083906512410522 32.0 21.0 34.0 2.0 35.0 100-101 21.83578529192023 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.251096660154591 1101 2 0.2466828761723292 1101 3 0.2289529720749215 1101 4 0.17067166742424433 1101 5 0.22094458535034533 1101 6 0.19976792807340615 1101 7 0.17527883189934101 1101 8 0.1948138432211266 1101 9 0.11295590413597978 1101 10-11 0.18611756540023805 1101 12-13 0.21580338810066735 1101 14-15 0.3843830235186587 1101 16-17 0.3794810705068983 1101 18-19 0.3437303730603034 1101 20-21 0.22416531184904187 1101 22-23 0.16322167191721348 1101 24-25 0.18781929988644208 1101 26-27 0.15067591887355292 1101 28-29 0.14570609283778424 1101 30-31 0.23955215244780703 1101 32-33 0.09408504974929599 1101 34-35 0.147055278397346 1101 36-37 0.07594435602153027 1101 38-39 0.07998336324889976 1101 40-41 0.041749111755855495 1101 42-43 -0.002222187416030863 1101 44-45 -0.025536074844964674 1101 46-47 -0.05499521971366761 1101 48-49 0.07260808051839263 1101 50-51 0.0358162228415253 1101 52-53 0.0016335003010254923 1101 54-55 0.012485618981521895 1101 56-57 0.07379998576997338 1101 58-59 -0.1009384331380403 1101 60-61 -0.1211784812114658 1101 62-63 -0.21654548323973444 1101 64-65 -0.09005892637195245 1101 66-67 -0.2795618930404231 1101 68-69 -0.38935853684053967 1101 70-71 -0.36849257558993287 1101 72-73 -0.20228999514938906 1101 74-75 -0.21672076047412148 1101 76-77 -0.39365126458972455 1101 78-79 -0.2550769445886161 1101 80-81 -0.1877088962132092 1101 82-83 -0.1937965746823913 1101 84-85 -0.20722607175956398 1101 86-87 -0.2943430186814133 1101 88-89 -0.2832362485488673 1101 90-91 -0.2626228735675795 1101 92-93 -0.4310759628869363 1101 94-95 -0.4751844668666152 1101 96-97 -0.3651865219584387 1101 98-99 -0.42275174426603 1101 100-101 0.1552257539739763 1102 1 0.23577836283508802 1102 2 0.25480366102549823 1102 3 0.18310459317501682 1102 4 0.15768805992725987 1102 5 0.16342132442678547 1102 6 0.10800191290365291 1102 7 0.1219582992385213 1102 8 0.15220288813811322 1102 9 0.19146796924745502 1102 10-11 0.2656458033016875 1102 12-13 0.28664722944874654 1102 14-15 0.2883961298788691 1102 16-17 0.33081128964676054 1102 18-19 0.28431184838557755 1102 20-21 0.1900964174832538 1102 22-23 0.11447236449090781 1102 24-25 0.1323209811688031 1102 26-27 0.28846011290881535 1102 28-29 0.2798342141957946 1102 30-31 0.2819653657987118 1102 32-33 0.33001434036278 1102 34-35 0.23885448934096587 1102 36-37 0.16168378591396504 1102 38-39 0.16210636433790881 1102 40-41 0.14294869716690783 1102 42-43 0.20252647997664752 1102 44-45 0.24060304171757707 1102 46-47 0.11916390028785884 1102 48-49 0.3237763807037197 1102 50-51 0.29510315851336344 1102 52-53 0.34413555414027286 1102 54-55 0.3175086888502676 1102 56-57 0.12082168004406668 1102 58-59 0.2727647823144679 1102 60-61 0.39495299315853316 1102 62-63 0.3531159676305222 1102 64-65 0.16880315115808742 1102 66-67 0.15906055398963304 1102 68-69 0.1400901744947447 1102 70-71 0.3608546378756152 1102 72-73 0.3341716219385553 1102 74-75 0.044821099635544925 1102 76-77 -0.02066354941424109 1102 78-79 0.20431960878254785 1102 80-81 0.25809195780180616 1102 82-83 0.13637312975580684 1102 84-85 0.028316571674839253 1102 86-87 0.22558423719830145 1102 88-89 0.029122370889048455 1102 90-91 -0.08929006563498021 1102 92-93 0.07907668990915084 1102 94-95 -0.012832295528660609 1102 96-97 0.3092584638079785 1102 98-99 0.3781612589245853 1102 100-101 1.0299580384163853 1103 1 0.2251367310546435 1103 2 0.2173721188583002 1103 3 0.1453862697856323 1103 4 0.14380942630557314 1103 5 0.22965307096681187 1103 6 0.17061286992414182 1103 7 0.10465230037811324 1103 8 0.09022871257526077 1103 9 0.2832659792023904 1103 10-11 0.241457231698206 1103 12-13 0.27876068697545975 1103 14-15 0.259567788924727 1103 16-17 0.24554496925233593 1103 18-19 0.32809860841703653 1103 20-21 0.16482541158280384 1103 22-23 0.07638726487093095 1103 24-25 0.15411912062265287 1103 26-27 0.15440125212250422 1103 28-29 0.07944501110854674 1103 30-31 0.16658701707463308 1103 32-33 0.12025353662190241 1103 34-35 0.20796175917431015 1103 36-37 0.2792637926949979 1103 38-39 0.13708576223861968 1103 40-41 0.25482533650538386 1103 42-43 0.1881320875148944 1103 44-45 0.23520099469298827 1103 46-47 0.3481655931917871 1103 48-49 0.2503844039852652 1103 50-51 0.3851520502381476 1103 52-53 0.29533306587757835 1103 54-55 0.2911722012374298 1103 56-57 0.20169839943640255 1103 58-59 0.28247543465933944 1103 60-61 0.2531423276283178 1103 62-63 0.3246289151669757 1103 64-65 0.271567246525791 1103 66-67 0.10442466960626007 1103 68-69 0.2859601010806401 1103 70-71 0.256242651927419 1103 72-73 0.1878069506538793 1103 74-75 0.18251299347469185 1103 76-77 0.25743547722486326 1103 78-79 0.1314156075016868 1103 80-81 0.2706185639414649 1103 82-83 0.28897517489677327 1103 84-85 0.5960744628674988 1103 86-87 0.6181479977524624 1103 88-89 0.5630998828147185 1103 90-91 0.6126475323721472 1103 92-93 0.5308027926512295 1103 94-95 0.5387332285651993 1103 96-97 0.7663254547713088 1103 98-99 0.6280276984580127 1103 100-101 0.731897284591458 1104 1 0.18575372244198363 1104 2 0.1182599344457742 1104 3 0.18130120208555667 1104 4 0.12659390743774424 1104 5 0.09977328446887412 1104 6 0.06097744868340982 1104 7 0.15005715601950698 1104 8 0.09140206345867341 1104 9 0.07874757462025173 1104 10-11 0.13464836733099617 1104 12-13 0.06207605561375118 1104 14-15 0.15304204623961226 1104 16-17 0.21139370759544818 1104 18-19 0.07854682964935478 1104 20-21 0.03248039337137243 1104 22-23 0.08617739636868293 1104 24-25 0.09218017382097088 1104 26-27 0.12109762296733351 1104 28-29 0.10404869750937706 1104 30-31 0.06106507487542956 1104 32-33 0.03416166372568341 1104 34-35 -0.08252759864070924 1104 36-37 0.022752143363931054 1104 38-39 0.11280462784488776 1104 40-41 0.12689071963133358 1104 42-43 0.20538452750522396 1104 44-45 0.1104571872554061 1104 46-47 0.036806481267852575 1104 48-49 0.2523673311324828 1104 50-51 0.11615873152749145 1104 52-53 0.27333715778943457 1104 54-55 0.26727162922289693 1104 56-57 0.07494454156743302 1104 58-59 0.06948094403162486 1104 60-61 0.30383311705090676 1104 62-63 0.24010810175044384 1104 64-65 0.21285005599141016 1104 66-67 0.2095159251191845 1104 68-69 0.30019797323867436 1104 70-71 0.21406032921123952 1104 72-73 0.44777424977101177 1104 74-75 0.2748892237755136 1104 76-77 0.2715623470437336 1104 78-79 0.22377349672708746 1104 80-81 0.35721400988231267 1104 82-83 0.2566383056191732 1104 84-85 0.5106729193734019 1104 86-87 0.7118111502212834 1104 88-89 0.5152235821964908 1104 90-91 0.4699657349754318 1104 92-93 0.5679401845120715 1104 94-95 0.63639754339221 1104 96-97 0.9248820204999291 1104 98-99 0.9986005840396928 1104 100-101 1.4070703867053638 1105 1 -0.03748014699302615 1105 2 0.07643081921045791 1105 3 0.05053588312132007 1105 4 0.03958653967815451 1105 5 0.08969334008236274 1105 6 0.05056961074329536 1105 7 0.05840619913536926 1105 8 0.10076458017439904 1105 9 0.04711226759032172 1105 10-11 0.08245422010251957 1105 12-13 0.08774849423430453 1105 14-15 0.16733195550813207 1105 16-17 0.09229172499520644 1105 18-19 0.055817299881240956 1105 20-21 0.0689895855204199 1105 22-23 0.10117874047541164 1105 24-25 0.060893735258630954 1105 26-27 -0.0768627592726503 1105 28-29 -0.051453322075204255 1105 30-31 -0.014819167570607306 1105 32-33 0.08442397856855166 1105 34-35 0.10728509965783672 1105 36-37 0.05054690083400004 1105 38-39 -0.06635709473490436 1105 40-41 0.14159028181210687 1105 42-43 0.09913845495086093 1105 44-45 0.2075522044719591 1105 46-47 0.14318482419828626 1105 48-49 0.09611064270327319 1105 50-51 0.1458412312578119 1105 52-53 0.23419754020063976 1105 54-55 0.06426827837336901 1105 56-57 -0.007798069698061738 1105 58-59 0.029829795376848267 1105 60-61 0.2631276579228796 1105 62-63 0.17386418661580194 1105 64-65 0.1538929972051477 1105 66-67 0.07093466830234263 1105 68-69 0.11864810687884386 1105 70-71 0.25031568209490374 1105 72-73 0.4526241635598325 1105 74-75 0.25738159147517337 1105 76-77 0.13262879728353028 1105 78-79 0.21634495269432108 1105 80-81 0.2453068508445213 1105 82-83 0.5213519937624405 1105 84-85 0.5414923450545714 1105 86-87 0.6743080039841836 1105 88-89 0.6037997879115977 1105 90-91 0.5688106593030788 1105 92-93 0.4361573681889652 1105 94-95 0.6823510116156442 1105 96-97 0.8361893826091311 1105 98-99 0.606863672814093 1105 100-101 1.0360810899043393 1106 1 0.1370118714927422 1106 2 0.16816541569460242 1106 3 0.13853057293332682 1106 4 0.13268514325653769 1106 5 0.1624906634119796 1106 6 -0.014528639222113782 1106 7 0.07060010290769014 1106 8 0.05633046331529812 1106 9 0.08704581032779402 1106 10-11 0.05680379449334794 1106 12-13 0.13653821888068052 1106 14-15 0.31169157890033006 1106 16-17 0.19325234859267937 1106 18-19 0.22091485061196892 1106 20-21 0.10975696562289983 1106 22-23 0.04780565731492459 1106 24-25 0.12223647063476051 1106 26-27 -0.008564761431280488 1106 28-29 -0.16405195786833104 1106 30-31 0.024599313901418896 1106 32-33 0.0070642737654651455 1106 34-35 0.10263831056849426 1106 36-37 -0.10678177792709675 1106 38-39 0.16158816917374708 1106 40-41 0.2121567967614979 1106 42-43 0.2151216363588091 1106 44-45 0.1612145460391119 1106 46-47 0.14038863462776874 1106 48-49 0.14701592686975573 1106 50-51 0.2587178128392722 1106 52-53 0.030177340309414546 1106 54-55 -0.07542020757875179 1106 56-57 -0.15680660485633524 1106 58-59 8.545088279774404E-4 1106 60-61 -0.08805484782457995 1106 62-63 -0.004362619145290125 1106 64-65 -0.06164859252488952 1106 66-67 0.15972348893815536 1106 68-69 -0.0033954414574992597 1106 70-71 0.18301222073336376 1106 72-73 0.20224270606506067 1106 74-75 0.11552447908468366 1106 76-77 0.017161051731040544 1106 78-79 0.0027443635391506405 1106 80-81 0.07701155005123894 1106 82-83 -0.027467323494821727 1106 84-85 0.413889381498187 1106 86-87 0.6399572259107877 1106 88-89 0.487820976720446 1106 90-91 0.41611416062088935 1106 92-93 0.7082871471458958 1106 94-95 0.6892910407537514 1106 96-97 0.9468803563383865 1106 98-99 0.9014327669405695 1106 100-101 1.4653412646430866 1107 1 0.05948326770437973 1107 2 0.08740606417585184 1107 3 0.05890456144074108 1107 4 0.1329751108992383 1107 5 0.042659293048316727 1107 6 -0.04820586487947054 1107 7 0.04083370373036388 1107 8 0.025233800210124002 1107 9 -0.009467951551478393 1107 10-11 0.027288376759329935 1107 12-13 0.0030268651777021205 1107 14-15 0.04643956737436383 1107 16-17 0.10837852063865228 1107 18-19 -0.002561579926208424 1107 20-21 -0.02701160407682579 1107 22-23 5.708874045069479E-4 1107 24-25 0.08216513460885011 1107 26-27 0.06841853379189189 1107 28-29 0.07465263161695646 1107 30-31 0.1224794611073321 1107 32-33 0.20480975043216176 1107 34-35 0.22640822124401438 1107 36-37 0.21892418990094598 1107 38-39 0.1783567189444355 1107 40-41 0.12493246219862186 1107 42-43 0.1199296062838826 1107 44-45 0.24992901285861535 1107 46-47 0.22745646388863605 1107 48-49 0.32756381873772966 1107 50-51 0.3027807502663009 1107 52-53 0.4323445868698528 1107 54-55 0.3088130758738359 1107 56-57 0.23383411651540342 1107 58-59 0.2740611997067788 1107 60-61 0.34565553478573463 1107 62-63 0.3297844213537715 1107 64-65 0.3614478907206333 1107 66-67 0.48684890318209284 1107 68-69 0.4449604245704748 1107 70-71 0.37310917804110844 1107 72-73 0.530268726426069 1107 74-75 0.5322408022949752 1107 76-77 0.24384057844605422 1107 78-79 0.4051390503554124 1107 80-81 0.5926186722942752 1107 82-83 0.5910549575540749 1107 84-85 0.657521303448064 1107 86-87 0.5129809805881962 1107 88-89 0.42199327728734914 1107 90-91 0.5100267458393652 1107 92-93 0.6113865126299309 1107 94-95 0.7407428594163576 1107 96-97 0.9091390996475184 1107 98-99 0.8151140830349455 1107 100-101 1.0595164150638006 1108 1 0.12494802644800984 1108 2 0.1371701718679148 1108 3 0.10424590830455571 1108 4 0.1373775389927161 1108 5 0.15260777432987993 1108 6 0.09296991721721781 1108 7 0.08840682891209894 1108 8 0.12988195461690566 1108 9 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-1.3447866801942965 2316 54-55 -1.275606042102332 2316 56-57 -1.094466641315556 2316 58-59 -1.7573462645865945 2316 60-61 -1.4157637551570517 2316 62-63 -1.1717297093549917 2316 64-65 -1.0678137600175255 2316 66-67 -0.9520676129156307 2316 68-69 -1.4049327441146424 2316 70-71 -1.5864898570612809 2316 72-73 -1.1502573500731579 2316 74-75 -1.0026760765816363 2316 76-77 -1.0593536425334023 2316 78-79 -1.2196250844758367 2316 80-81 -0.8347837366517616 2316 82-83 -1.1107402090061917 2316 84-85 -1.0921041391303987 2316 86-87 -1.0049659736003669 2316 88-89 -1.114364705379355 2316 90-91 -1.2735479689434044 2316 92-93 -1.25900366312937 2316 94-95 -1.361131458900914 2316 96-97 -1.324426310092388 2316 98-99 -1.9964370087174892 2316 100-101 -2.6937185596476816 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 41.0 5 88.0 6 189.0 7 427.0 8 999.0 9 1779.0 10 3007.0 11 4750.0 12 6603.0 13 9273.0 14 11864.0 15 15027.0 16 18873.0 17 23389.0 18 21391.0 19 15769.0 20 15323.0 21 16079.0 22 16947.0 23 19368.0 24 22741.0 25 27304.0 26 33564.0 27 41974.0 28 51937.0 29 63875.0 30 78603.0 31 97198.0 32 120250.0 33 152881.0 34 200159.0 35 269291.0 36 378718.0 37 478491.0 38 273295.0 39 14140.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58955935256106 18.767953145782336 14.43381027426898 23.208677227387618 2 16.337593817195824 20.074794303690098 38.8321263448146 24.75548553429948 3 20.086669383483482 23.62437200220485 29.037556333702273 27.251402280609394 4 13.478282386447086 16.341490475795172 35.66300662192451 34.51722051583323 5 14.307580125474 36.990962822769866 33.419316163109016 15.282140888647113 6 33.532512563776734 35.157502187086926 16.61359921073301 14.69638603840333 7 30.116039375562487 31.922701612179043 19.448199344272762 18.513059667985704 8 27.40216377928621 34.496985970715386 18.652938039986974 19.44791221001143 9 26.656359154794668 16.63072074890965 19.502669203900357 37.21025089239532 10-11 25.87321840218134 26.161430961488115 26.331069884611207 21.634280751719338 12-13 26.729107174572007 23.198434696887155 27.138791938808705 22.933666189732136 14-15 23.1124111269489 24.910755282993904 25.496680349958623 26.48015324009857 16-17 22.922032530529922 28.19381106159345 26.05206185865309 22.832094549223534 18-19 22.786949659648915 26.58274579342981 28.468441070101886 22.161863476819388 20-21 23.456487346824094 25.67642048901348 28.566907299442533 22.300184864719892 22-23 23.033833650221982 25.65926009294336 28.515747849228053 22.791158407606606 24-25 23.156181952861093 25.805290994310383 28.6377880728731 22.400738979955428 26-27 22.969137478974424 26.03044525228595 28.52294249693534 22.47747477180429 28-29 22.563864323385 26.29776104689357 28.663832154747908 22.474542474973518 30-31 22.757518872388154 25.905274969433208 28.62706157480199 22.710144583376653 32-33 22.701323416655725 26.02397452870968 28.577549613655894 22.697152440978698 34-35 23.30962615293127 25.938030069006253 28.249639802519987 22.50270397554249 36-37 23.321416633823404 25.736518880302523 28.329683158141556 22.612381327732518 38-39 22.92465880264433 26.021716259816056 28.41576508188896 22.63785985565065 40-41 23.06633453204319 25.94587227344394 28.389707817715482 22.598085376797393 42-43 23.095749400267852 26.041915494891526 28.01170707487367 22.850628029966952 44-45 23.09620774128764 26.13692329674031 27.851757159425887 22.91511180254616 46-47 23.328087106914662 26.442497746557176 27.42984194869873 22.79957319782943 48-49 23.581611339566173 26.405986433979745 27.2179281716524 22.79447405480168 50-51 23.350588588383644 26.61048880842955 27.145188194428233 22.893734408758572 52-53 23.638645424209905 26.735175571946325 26.865742527471344 22.76043647637243 54-55 23.180328405363746 26.583516991397538 27.03738722091092 23.198767382327798 56-57 23.324230320397714 26.903704253667698 26.862051133745236 22.910014292189352 58-59 23.57902897552119 26.47210993308428 26.902678784578644 23.046182306815886 60-61 23.346021659773612 26.544370121496165 27.377983388411884 22.731624830318335 62-63 23.264821176704924 26.579114965545003 27.2885124666472 22.86755139110287 64-65 23.135701945430856 26.64067888894566 27.138792376670157 23.084826788953325 66-67 23.12748350450124 26.875549783439208 26.874332477094686 23.12263423496487 68-69 23.143319310006 26.55876646869576 27.1537368289347 23.144177392363545 70-71 23.21966256966607 26.712559618632326 26.956471937910614 23.11130587379099 72-73 23.315943065497667 26.586057081372406 26.98725582850684 23.110744024623088 74-75 23.16345938365009 27.024990486210044 26.651343494812664 23.160206635327203 76-77 23.2821386158688 26.890726620687673 26.654412608416187 23.17272215502734 78-79 23.365896035777528 26.83212848872912 26.65664553141121 23.145329944082146 80-81 23.292754120707773 27.08050211910709 26.61357006924645 23.013173690938686 82-83 23.279898225939533 27.051977181470065 26.526421298146186 23.14170329444422 84-85 23.294697835890492 26.974717988681224 26.438161912183656 23.29242226324463 86-87 23.28315595593502 26.85368145830585 26.577851238481877 23.28531134727725 88-89 23.258045177684533 26.937583183726566 26.31495417571078 23.489417462878123 90-91 23.20862979297353 26.943067970220643 26.52481856061113 23.3234836761947 92-93 23.18965956032572 26.80779545075746 26.660585307529473 23.341959681387348 94-95 23.042069259428928 26.74457529021112 26.65808334406249 23.555272106297465 96-97 23.073639598151956 26.702562451098167 26.62903126303342 23.594766687716458 98-99 22.96334393435023 26.758945893285578 26.308983338587588 23.968726833776604 100-101 22.71099447043879 26.848661256772182 26.47380756232487 23.966536710464155 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 290.0 1 235.5 2 238.5 3 319.0 4 286.5 5 266.5 6 436.5 7 864.5 8 1375.5 9 1675.5 10 1672.0 11 1568.5 12 1671.0 13 1882.5 14 2123.0 15 2373.5 16 2618.0 17 2893.5 18 3100.5 19 3300.0 20 3692.5 21 4259.0 22 4864.5 23 5872.5 24 7179.5 25 8717.0 26 10973.0 27 13738.0 28 16409.5 29 19704.0 30 23867.5 31 28311.5 32 33658.0 33 38051.5 34 41981.0 35 46169.0 36 51102.5 37 56145.5 38 58896.5 39 60854.0 40 63982.5 41 69524.0 42 74618.5 43 79076.5 44 83104.5 45 87306.0 46 90571.5 47 94187.5 48 104949.0 49 106441.5 50 99018.5 51 96151.0 52 94056.5 53 93024.0 54 92147.5 55 87967.0 56 80172.0 57 72432.5 58 66733.0 59 60108.0 60 51369.0 61 42730.0 62 35401.5 63 28078.0 64 21042.5 65 16371.0 66 12564.0 67 10145.0 68 7657.0 69 5111.5 70 3810.5 71 2913.0 72 2384.5 73 1956.5 74 1515.0 75 1210.5 76 865.0 77 482.0 78 298.0 79 224.0 80 147.0 81 100.5 82 72.0 83 44.5 84 23.0 85 14.0 86 7.5 87 3.0 88 5.0 89 3.5 90 0.5 91 1.0 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019316607173645126 2 0.013769069163032164 3 0.009299110478221722 4 0.0 5 0.0030730965958071788 6 0.0 7 3.9910345400093234E-5 8 0.0 9 0.0 10-11 0.0 12-13 0.0012372207074028902 14-15 2.1950689970051275E-4 16-17 1.9955172700046617E-5 18-19 0.0016562793341038692 20-21 0.0 22-23 1.5964138160037294E-4 24-25 0.0 26-27 0.0014567276071034028 28-29 0.0027937241780065262 30-31 0.001496637952503496 32-33 0.008261441497819298 34-35 0.0018358758884042886 36-37 0.001137444843902657 38-39 0.0011174896712026106 40-41 2.1950689970051275E-4 42-43 0.004889017311511421 44-45 0.0015165931252035427 46-47 2.1950689970051275E-4 48-49 6.984310445016316E-4 50-51 9.977586350023308E-5 52-53 1.9955172700046616E-4 54-55 0.0021551586516050346 56-57 0.016024003678137434 58-59 0.026899572799662836 60-61 0.022250017560551977 62-63 0.015624900224136498 64-65 0.018518400265643258 66-67 0.0033524690136078317 68-69 0.0011574000166027036 70-71 9.977586350023308E-5 72-73 0.0 74-75 0.0016961896795039623 76-77 0.0020553827881048016 78-79 0.009758079450322794 80-81 0.017301134730940415 82-83 0.018318848538642792 84-85 0.030012579740870106 86-87 0.010795748430725218 88-89 0.03969083850039272 90-91 0.04507873512940531 92-93 0.027677824534964656 94-95 0.030271996985970714 96-97 0.049349142087215275 98-99 0.051743762811220874 100-101 0.057430987030734154 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2505616.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.865135137349554 #Duplication Level Percentage of deduplicated Percentage of total 1 78.29651590757656 43.74045441960404 2 12.69019906410985 14.178793712727272 3 3.7337329939741997 6.257564948254482 4 1.6492288222131908 3.6853756410140694 5 0.8850602582968596 2.4722005467225783 6 0.5577233378603376 1.869437378329285 7 0.37700241988549427 1.4742903793807656 8 0.27296421495721673 1.2199346204996357 9 0.1993152113270264 1.0021294094142343 >10 1.181740597083359 12.162968579088188 >50 0.09948950308179483 3.8527745983390504 >100 0.05258741841845926 5.283972270389454 >500 0.0030079121138833547 1.1285712978403788 >1k 0.0012891051916607173 1.1018053378885448 >5k 1.4323391018452415E-4 0.5697268605081114 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7591 0.3029594319321077 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6649 0.2653638865652199 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3945 0.1574463126033678 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3621 0.14451536069373758 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.9910345400093234E-5 0.0 12-13 0.0 0.0 0.0 3.9910345400093234E-5 0.0 14-15 0.0 0.0 0.0 7.982069080018647E-5 0.0 16-17 0.0 0.0 0.0 1.3968620890032632E-4 0.0 18-19 0.0 1.9955172700046617E-5 0.0 2.793724178006526E-4 0.0 20-21 0.0 3.9910345400093234E-5 0.0 2.9932759050069923E-4 0.0 22-23 0.0 3.9910345400093234E-5 0.0 4.3901379940102555E-4 0.0 24-25 0.0 7.982069080018647E-5 0.0 5.387896629012586E-4 0.0 26-27 0.0 1.197310362002797E-4 0.0 7.582965626017714E-4 0.0 28-29 0.0 1.197310362002797E-4 0.0 0.0013769069163032165 0.0 30-31 0.0 1.197310362002797E-4 0.0 0.002394620724005594 0.0 32-33 0.0 1.197310362002797E-4 0.0 0.004270406957809976 0.0 34-35 0.0 1.5964138160037294E-4 0.0 0.005886775946513751 0.0 36-37 0.0 1.9955172700046616E-4 0.0 0.008500903570219858 0.0 38-39 0.0 2.1950689970051278E-4 0.0 0.012292386383228715 0.0 40-41 0.0 2.394620724005594E-4 0.0 0.020154724427047084 0.0 42-43 0.0 2.394620724005594E-4 0.0 0.028076927988965588 0.0 44-45 0.0 2.394620724005594E-4 0.0 0.03691706949508623 0.0 46-47 0.0 2.394620724005594E-4 0.0 0.05268165592812306 0.0 48-49 0.0 2.394620724005594E-4 0.0 0.06355722504964847 0.0 50-51 0.0 2.394620724005594E-4 0.0 0.07503144935217526 0.0 52-53 0.0 2.394620724005594E-4 0.0 0.08890029437870767 0.0 54-55 0.0 2.394620724005594E-4 0.0 0.10769806706215158 1.9955172700046617E-5 56-57 0.0 2.394620724005594E-4 0.0 0.15088106078505248 3.9910345400093234E-5 58-59 0.0 2.394620724005594E-4 0.0 0.19278692345515036 3.9910345400093234E-5 60-61 0.0 2.9932759050069923E-4 0.0 0.22369748596752256 3.9910345400093234E-5 62-63 0.0 3.591931086008391E-4 0.0 0.2605946002899088 3.9910345400093234E-5 64-65 0.0 3.591931086008391E-4 0.0 0.29960696291849986 3.9910345400093234E-5 66-67 0.0 3.591931086008391E-4 0.0 0.36368302245834955 3.9910345400093234E-5 68-69 0.0 3.591931086008391E-4 0.0 0.4425059546235337 3.9910345400093234E-5 70-71 0.0 3.591931086008391E-4 0.0 0.48473110005683234 3.9910345400093234E-5 72-73 0.0 3.991034540009323E-4 0.0 0.532503783500744 3.9910345400093234E-5 74-75 0.0 4.3901379940102555E-4 0.0 0.6182312054201442 3.9910345400093234E-5 76-77 0.0 4.3901379940102555E-4 0.0 0.723015817268089 5.9865518100139854E-5 78-79 0.0 4.3901379940102555E-4 0.0 0.8259445980549294 7.982069080018647E-5 80-81 0.0 4.3901379940102555E-4 0.0 0.9070424199079188 7.982069080018647E-5 82-83 0.0 4.3901379940102555E-4 0.0 1.0339772734529153 7.982069080018647E-5 84-85 0.0 4.789241448011188E-4 0.0 1.2446440316473075 7.982069080018647E-5 86-87 0.0 4.789241448011188E-4 0.0 1.4587031692007075 7.982069080018647E-5 88-89 0.0 4.789241448011188E-4 0.0 1.7251845454371302 7.982069080018647E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9840 0.0 25.880646 1 GGTATCA 10635 0.0 25.82235 1 TACCTGG 2225 0.0 25.62048 2 GTATCAA 14500 0.0 25.361702 1 TACATGG 9920 0.0 24.757963 2 GTACCTG 2440 0.0 23.756115 1 ACATGGG 9985 0.0 23.403189 3 GAGTACT 9315 0.0 21.845232 12-13 TCAACGC 17440 0.0 21.022135 4 ATCAACG 17540 0.0 20.904785 3 CAACGCA 17670 0.0 20.802256 5 TATCAAC 17680 0.0 20.769844 2 GTACTTT 9665 0.0 20.75934 14-15 AACGCAG 17930 0.0 20.551939 6 AGTACTT 9790 0.0 20.154734 12-13 CATGGGG 5205 0.0 19.1604 4 AGAGTAC 15610 0.0 18.92202 10-11 ACGCAGA 19940 0.0 18.480253 7 GTGTAGC 1270 0.0 18.33149 1 CGCAGAG 20160 0.0 18.231474 8 >>END_MODULE