FastQCFastQC Report
Thu 26 May 2016
SRR936627_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936627_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2426816
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56130.23129071178037397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45370.1869527809277671No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38820.15996268361507424No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27100.1116689522403017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26240.10812521427252827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA116450.026.149311
GTACATG85000.025.8763941
TACATGG88250.024.6547972
ACATGGG89700.023.7805793
GGTATCA84950.023.2073941
ATCAACG137700.021.8737813
TCAACGC138400.021.7979244
TATCAAC140800.021.4925232
CAACGCA139200.021.4678675
GAGTACT66350.021.23142612-13
AACGCAG141350.021.1081566
AGTACTT70650.020.74619112-13
AGAGTAC112600.020.44413410-11
CATGGGG57300.019.9820444
GTACTTT70550.019.53134514-15
ACGCAGA156450.019.1020267
CGCAGAG158100.018.9627728
GTACCTG21700.017.9512961
TACCTGG21500.017.6767392
GCAGAGT167700.017.567419
Produced by FastQC (version 0.11.5)