##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936627_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2426816 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.320609803132992 31.0 31.0 34.0 28.0 34.0 2 31.409193362826024 31.0 31.0 34.0 28.0 34.0 3 31.423303620876077 31.0 31.0 34.0 28.0 34.0 4 35.04751410902186 37.0 35.0 37.0 32.0 37.0 5 35.051704373137476 37.0 35.0 37.0 32.0 37.0 6 35.029231305546034 37.0 35.0 37.0 32.0 37.0 7 34.93071580210449 37.0 35.0 37.0 32.0 37.0 8 34.884805852606874 37.0 35.0 37.0 32.0 37.0 9 36.378912946016506 39.0 37.0 39.0 32.0 39.0 10-11 36.23458453380891 39.0 35.0 39.0 32.0 39.0 12-13 36.13018106852765 39.0 35.0 39.0 31.0 39.0 14-15 37.23012622300166 39.5 36.5 41.0 31.5 41.0 16-17 37.11172849527941 39.0 36.0 41.0 31.0 41.0 18-19 37.05177710217833 39.0 36.0 41.0 31.0 41.0 20-21 36.901347279727844 39.0 36.0 41.0 30.5 41.0 22-23 37.025357505472186 39.0 36.0 41.0 31.0 41.0 24-25 37.068673315158634 39.0 36.0 41.0 31.0 41.0 26-27 36.98036089262639 39.0 36.0 41.0 30.5 41.0 28-29 36.83632339658219 39.0 36.0 41.0 30.0 41.0 30-31 36.62832555084522 39.0 36.0 41.0 30.0 41.0 32-33 36.455556581133465 39.0 35.5 41.0 30.0 41.0 34-35 36.27668043230307 39.0 35.0 40.5 30.0 41.0 36-37 36.07896560761096 39.0 35.0 40.0 29.0 41.0 38-39 35.88027769719666 38.0 35.0 40.0 28.5 41.0 40-41 35.67441680786677 38.0 35.0 40.0 27.0 41.0 42-43 35.443303901078615 38.0 34.5 40.0 27.0 41.0 44-45 35.187660498364934 38.0 34.0 40.0 26.0 41.0 46-47 35.04399138624436 38.0 34.0 40.0 25.5 41.0 48-49 34.90863481203355 38.0 34.0 40.0 24.5 41.0 50-51 33.95399342183338 37.5 33.0 39.5 23.5 40.5 52-53 34.06267389039795 37.5 33.0 39.5 24.0 40.5 54-55 34.49987184854558 38.0 34.0 40.0 24.0 41.0 56-57 34.381050108454865 38.0 34.0 40.0 23.5 41.0 58-59 34.14440876440571 38.0 33.0 40.0 23.0 41.0 60-61 33.83952285628577 37.0 33.0 40.0 22.5 41.0 62-63 33.455431932210765 37.0 33.0 40.0 22.0 41.0 64-65 33.08410361560168 36.0 32.0 39.0 20.5 41.0 66-67 32.61678553297819 36.0 31.5 39.0 19.5 41.0 68-69 32.2176992404863 35.0 31.0 39.0 18.0 40.0 70-71 31.822966182850287 35.0 31.0 38.0 17.5 40.0 72-73 31.423193600174056 35.0 31.0 37.0 14.0 39.5 74-75 30.97492372722118 35.0 30.5 37.0 11.5 39.0 76-77 30.504628904704767 34.5 30.0 36.0 7.5 39.0 78-79 30.008509503810753 34.0 29.5 36.0 7.0 38.5 80-81 29.538976172894856 34.0 29.0 35.0 2.0 37.0 82-83 29.12201357663704 34.0 29.0 35.0 2.0 37.0 84-85 28.737378523958967 34.0 29.0 35.0 2.0 36.0 86-87 28.364942995266226 34.0 27.5 35.0 2.0 36.0 88-89 27.947043780822277 34.0 27.0 35.0 2.0 35.5 90-91 27.527513210725495 33.0 26.0 35.0 2.0 35.0 92-93 27.0549269083441 33.0 25.5 35.0 2.0 35.0 94-95 26.530766444592423 33.0 25.0 35.0 2.0 35.0 96-97 25.90346157269443 32.5 23.5 35.0 2.0 35.0 98-99 25.096855715472454 32.0 18.5 35.0 2.0 35.0 100-101 22.111585509573036 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3382748114751486 1101 2 0.3877682368477551 1101 3 0.2660684715286017 1101 4 0.3198578592386241 1101 5 0.3053957885576253 1101 6 0.4390626553825001 1101 7 0.536030634512052 1101 8 0.4721330757346536 1101 9 0.573240039891239 1101 10-11 0.6323029134428353 1101 12-13 0.6129001168260402 1101 14-15 0.6247488155582346 1101 16-17 0.8038107126466869 1101 18-19 0.5846533632848008 1101 20-21 0.6938165276427739 1101 22-23 0.563647106825762 1101 24-25 0.5448785935297238 1101 26-27 0.6307332304452089 1101 28-29 0.5812470761556412 1101 30-31 0.5998681755045041 1101 32-33 0.6176176717202111 1101 34-35 0.5037405364307759 1101 36-37 0.3943230338405428 1101 38-39 0.30361778629163183 1101 40-41 0.283118655019436 1101 42-43 0.37375539179538464 1101 44-45 0.2820493052326256 1101 46-47 0.45002303805731003 1101 48-49 0.6269484282648818 1101 50-51 0.49228653124966826 1101 52-53 0.5352431634215336 1101 54-55 0.41535024802278286 1101 56-57 0.37496225623281987 1101 58-59 0.40329523281184265 1101 60-61 0.4254331636737234 1101 62-63 0.3989668292593791 1101 64-65 0.3800555990024108 1101 66-67 0.4455484655373141 1101 68-69 0.4266425008869561 1101 70-71 0.41245970307234003 1101 72-73 0.3186674048489486 1101 74-75 0.2820397012132254 1101 76-77 0.318933633925532 1101 78-79 0.27919744590426276 1101 80-81 0.2848945176921589 1101 82-83 0.21288886404362017 1101 84-85 0.35374685126033256 1101 86-87 0.45077638221811256 1101 88-89 0.47676443180983696 1101 90-91 0.42949582919796114 1101 92-93 0.29668777381429123 1101 94-95 0.34892568627314446 1101 96-97 0.19752277102449867 1101 98-99 0.22911022464165143 1101 100-101 0.4572028100176162 1102 1 0.22465885728341206 1102 2 0.2985430471007682 1102 3 0.3144884452839669 1102 4 0.3172682505832185 1102 5 0.3159831786309084 1102 6 0.2513097135954041 1102 7 0.25785796926727045 1102 8 0.43132167175784986 1102 9 0.42714461434033524 1102 10-11 0.45018930986460504 1102 12-13 0.49128676007266137 1102 14-15 0.45222507358509745 1102 16-17 0.29426145180151764 1102 18-19 0.23039853624592865 1102 20-21 0.3925227714839963 1102 22-23 0.14677643660003525 1102 24-25 0.2713608717084526 1102 26-27 0.06826305748091954 1102 28-29 0.008669713396635359 1102 30-31 0.18869013593145922 1102 32-33 0.11096918678524048 1102 34-35 0.02932918114962746 1102 36-37 -0.07582985106760276 1102 38-39 -0.09665620253792184 1102 40-41 -0.22351328652658253 1102 42-43 -0.1619444838513715 1102 44-45 0.025343202578497426 1102 46-47 0.05503813484686759 1102 48-49 0.2877460014633755 1102 50-51 0.34376352122677645 1102 52-53 0.4422310530293885 1102 54-55 0.3002563756561685 1102 56-57 0.30830578500840744 1102 58-59 0.2643247039121235 1102 60-61 0.25999794815314203 1102 62-63 0.4180092170841547 1102 64-65 0.4150161367054892 1102 66-67 0.5772078268033738 1102 68-69 0.6300196001135774 1102 70-71 0.662509680524078 1102 72-73 0.2586063786865225 1102 74-75 0.27261937777355527 1102 76-77 0.4689278058537738 1102 78-79 0.3874679280404969 1102 80-81 0.5244916621769384 1102 82-83 0.5336510318640535 1102 84-85 0.08672045217898372 1102 86-87 0.3049632677054035 1102 88-89 0.2902891195344246 1102 90-91 0.30604034714191997 1102 92-93 0.29282697620320874 1102 94-95 0.07537607738538199 1102 96-97 0.17026867429416725 1102 98-99 0.2866301357468224 1102 100-101 0.3374050586304058 1103 1 0.260672355301967 1103 2 0.13572507434991365 1103 3 0.24455832020814583 1103 4 0.395727423096325 1103 5 0.4227526529415684 1103 6 0.3844018758183765 1103 7 0.35544170896528016 1103 8 0.34846677800113923 1103 9 0.04103710308806541 1103 10-11 0.38642858464182694 1103 12-13 0.10775709257920596 1103 14-15 0.4475128375175714 1103 16-17 0.4651010434624112 1103 18-19 0.5164528307803664 1103 20-21 0.6924519515306216 1103 22-23 0.3683501684007453 1103 24-25 0.38517723663777303 1103 26-27 0.31850907668854234 1103 28-29 0.476080769678525 1103 30-31 0.42784300057186186 1103 32-33 0.36268190910399767 1103 34-35 0.5656735665760309 1103 36-37 0.6326600596964553 1103 38-39 0.6626155449940327 1103 40-41 0.3544312711673214 1103 42-43 0.48195757856355215 1103 44-45 0.5412135242229397 1103 46-47 0.7189096943141919 1103 48-49 0.648971835330812 1103 50-51 0.6279526232213755 1103 52-53 0.8028667842142454 1103 54-55 0.7894625651267617 1103 56-57 0.7628465103609159 1103 58-59 0.633311669504792 1103 60-61 0.6479945638547946 1103 62-63 0.760615813642282 1103 64-65 0.7113185923441279 1103 66-67 0.8000499073379288 1103 68-69 0.7720565971796702 1103 70-71 0.7540582068827 1103 72-73 0.6240184502070427 1103 74-75 0.5224885300238604 1103 76-77 0.5730866847210052 1103 78-79 0.47678476096912803 1103 80-81 0.5463327749151077 1103 82-83 0.21397989947869078 1103 84-85 0.39246855725554397 1103 86-87 0.41370013253099813 1103 88-89 0.4544796740503365 1103 90-91 0.42467044656331154 1103 92-93 0.3285153740047875 1103 94-95 0.4752335725797856 1103 96-97 0.5738793853542283 1103 98-99 0.5595880809303004 1103 100-101 0.5663529435138912 1104 1 0.45414901170491717 1104 2 0.4800972067454019 1104 3 0.4421934355329711 1104 4 0.5142867235665278 1104 5 0.5416164783679918 1104 6 0.485966285482057 1104 7 0.5068814030751625 1104 8 0.522047320767129 1104 9 0.5944890074495746 1104 10-11 0.704272258053507 1104 12-13 0.6893745246366052 1104 14-15 0.5264507530356823 1104 16-17 0.41905893890111656 1104 18-19 0.5079059708991451 1104 20-21 0.29545359775749347 1104 22-23 0.4485769452761943 1104 24-25 0.477369894068012 1104 26-27 0.29971892495447605 1104 28-29 0.374205971207239 1104 30-31 0.3090471035811504 1104 32-33 0.34295037862165145 1104 34-35 0.3572199089888457 1104 36-37 0.378474686355581 1104 38-39 0.25642076155529026 1104 40-41 0.0721373614951375 1104 42-43 0.35950105704499435 1104 44-45 0.18688562635040995 1104 46-47 -0.010921654731362196 1104 48-49 0.2531749781179613 1104 50-51 0.3127349010548741 1104 52-53 0.23710645813667952 1104 54-55 0.18578637833265788 1104 56-57 0.02187898278862832 1104 58-59 0.28784859720772005 1104 60-61 0.3702639165080086 1104 62-63 0.4850791691172631 1104 64-65 0.3573532649679336 1104 66-67 0.37818348692666603 1104 68-69 0.512818033435849 1104 70-71 0.27892798655605944 1104 72-73 0.17090771657862192 1104 74-75 0.20085331666071227 1104 76-77 0.15776662846492684 1104 78-79 0.2603119242394385 1104 80-81 0.3018609702232631 1104 82-83 0.186203362553357 1104 84-85 0.24006705974649734 1104 86-87 0.17933760762774753 1104 88-89 0.08328055333814532 1104 90-91 0.16741748831617542 1104 92-93 -0.05359683533760773 1104 94-95 -0.02185716650135916 1104 96-97 0.10156460432427394 1104 98-99 0.009959934759901756 1104 100-101 -0.04998323253552783 1105 1 0.30509561160349463 1105 2 0.25529897901529 1105 3 0.360165590857072 1105 4 0.35856226308022343 1105 5 0.24919892731154647 1105 6 0.46327745312293445 1105 7 0.5865678476332121 1105 8 0.6888135646924027 1105 9 0.6617514951564019 1105 10-11 0.6813495731221124 1105 12-13 0.4692009932985144 1105 14-15 0.4674940352663697 1105 16-17 0.5091738941734718 1105 18-19 0.5632343808043032 1105 20-21 0.5404846925529867 1105 22-23 0.25301932289369233 1105 24-25 0.35708569851880867 1105 26-27 0.30111772879548226 1105 28-29 0.2401242685798266 1105 30-31 0.11810842880047545 1105 32-33 0.055420941736180396 1105 34-35 0.13868786256325905 1105 36-37 0.2394165845650349 1105 38-39 0.11349392928240576 1105 40-41 -0.071621197529538 1105 42-43 -0.09301906885351485 1105 44-45 0.04052820353305009 1105 46-47 0.19726113215892838 1105 48-49 0.18334483433660864 1105 50-51 0.12327756631719211 1105 52-53 0.27546443940794063 1105 54-55 0.09105801893471721 1105 56-57 0.11578793427089806 1105 58-59 0.19398358290760598 1105 60-61 0.1882216998566406 1105 62-63 0.15891539840365 1105 64-65 0.21504011604758233 1105 66-67 0.34896300234471767 1105 68-69 0.28993168907099687 1105 70-71 0.2511759072391797 1105 72-73 0.04919597323746672 1105 74-75 0.19544493697775778 1105 76-77 0.13851043978963418 1105 78-79 0.1953390975387741 1105 80-81 0.2837541289576251 1105 82-83 -0.0037346121067969307 1105 84-85 0.16624889808641186 1105 86-87 0.058443013748977535 1105 88-89 0.0533217598449518 1105 90-91 0.0475071694431044 1105 92-93 -0.15647554202406155 1105 94-95 -0.10027702939271066 1105 96-97 0.1066785638240475 1105 98-99 0.19564914284352142 1105 100-101 0.18267851891552667 1106 1 0.4190991181974155 1106 2 0.35175644795144123 1106 3 0.35484035971590444 1106 4 0.3075859707043449 1106 5 0.2632753971951374 1106 6 0.48402298548270295 1106 7 0.5409886606838441 1106 8 0.5494645714768964 1106 9 0.6546315848903603 1106 10-11 0.6218659789722452 1106 12-13 0.6141104425890305 1106 14-15 0.5911831463121473 1106 16-17 0.6445251525179714 1106 18-19 0.6229466658620737 1106 20-21 0.4107635865601935 1106 22-23 0.5312359694137783 1106 24-25 0.5356519154266906 1106 26-27 0.5415415692781309 1106 28-29 0.5045345182885299 1106 30-31 0.6674337906221623 1106 32-33 0.5986729264465538 1106 34-35 0.6168870850141701 1106 36-37 0.405983062833954 1106 38-39 0.5229105496663848 1106 40-41 0.6546074715982826 1106 42-43 0.6380306224137655 1106 44-45 0.6845326902308599 1106 46-47 0.7517320394084379 1106 48-49 0.7497414279310846 1106 50-51 0.505911214372766 1106 52-53 0.35996355186941287 1106 54-55 0.4247735927855487 1106 56-57 0.47397670228030364 1106 58-59 0.5033315960192226 1106 60-61 0.6121503918535467 1106 62-63 0.7168210252889864 1106 64-65 0.7103824445566218 1106 66-67 0.7809851744727325 1106 68-69 0.7991925818367349 1106 70-71 0.6612949354117283 1106 72-73 0.5476005819037617 1106 74-75 0.785612316942256 1106 76-77 0.8788014217448321 1106 78-79 0.6982097129351139 1106 80-81 0.8575439729033043 1106 82-83 0.6833073254496966 1106 84-85 0.7679913050037648 1106 86-87 0.906000921553872 1106 88-89 0.7674635699879424 1106 90-91 0.7811966071901715 1106 92-93 0.8907850676005538 1106 94-95 0.707415697147411 1106 96-97 0.572195241925261 1106 98-99 0.777403964410162 1106 100-101 0.6675032436696817 1107 1 0.12147725604404869 1107 2 0.16900459528023504 1107 3 0.24424141936413335 1107 4 0.311589632472014 1107 5 0.26128406894030576 1107 6 0.4123490228407789 1107 7 0.3942804900109067 1107 8 0.38138377423052816 1107 9 0.508198270253196 1107 10-11 0.37600271128406604 1107 12-13 0.31756141281594097 1107 14-15 0.1929998776705375 1107 16-17 0.26164481023654673 1107 18-19 0.3060410830071305 1107 20-21 0.33488045326097904 1107 22-23 0.36534433717860537 1107 24-25 0.19428325154731851 1107 26-27 0.308832434746904 1107 28-29 0.2448065885205679 1107 30-31 0.35681473885313864 1107 32-33 0.07831614990451641 1107 34-35 0.18196740778007126 1107 36-37 0.2736623021158735 1107 38-39 0.28502764131709313 1107 40-41 0.3026266832158271 1107 42-43 0.19211558644784077 1107 44-45 0.13236129030489963 1107 46-47 0.04753121126496751 1107 48-49 0.05146639163743316 1107 50-51 0.09021477461502769 1107 52-53 0.2600661945841267 1107 54-55 0.42747589688358545 1107 56-57 0.23838150488674614 1107 58-59 0.35184398427981023 1107 60-61 0.3995439596264134 1107 62-63 0.43761205947020443 1107 64-65 0.32131195701862936 1107 66-67 0.39918369799676157 1107 68-69 0.34164256958366934 1107 70-71 0.2698899592511985 1107 72-73 0.21407857368191685 1107 74-75 0.280692021468294 1107 76-77 0.19424713916750136 1107 78-79 0.15954968452587437 1107 80-81 0.3374174348454311 1107 82-83 0.24544974258879648 1107 84-85 0.10584866440447271 1107 86-87 0.11829860628282418 1107 88-89 -0.004879060034234328 1107 90-91 -0.12774403507911458 1107 92-93 0.03049170906091092 1107 94-95 0.12910064658209564 1107 96-97 0.2274511373637651 1107 98-99 0.14452723473516826 1107 100-101 0.20708027821886432 1108 1 0.23902099016374123 1108 2 0.3543107531478107 1108 3 0.429204889899264 1108 4 0.30817387250843353 1108 5 0.38878583109307385 1108 6 0.26290278791042 1108 7 0.3960487322882642 1108 8 0.328354469972588 1108 9 0.38376029422455815 1108 10-11 0.35423902091752524 1108 12-13 0.17810281617460788 1108 14-15 0.4158039612978115 1108 16-17 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2316 94-95 -3.63673047819724 2316 96-97 -3.988017539023094 2316 98-99 -4.9118724394508995 2316 100-101 -5.872551712999098 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6226.0 3 2548.0 4 2658.0 5 2960.0 6 3288.0 7 3886.0 8 4954.0 9 6033.0 10 7308.0 11 8983.0 12 10553.0 13 12256.0 14 13730.0 15 15846.0 16 18341.0 17 21808.0 18 18965.0 19 16428.0 20 16951.0 21 18384.0 22 20812.0 23 23223.0 24 26484.0 25 30804.0 26 35443.0 27 41410.0 28 49529.0 29 58550.0 30 70612.0 31 86130.0 32 107224.0 33 135395.0 34 178102.0 35 240223.0 36 332283.0 37 425598.0 38 312740.0 39 40138.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.256222120383896 18.00446238703913 14.28610873915724 23.453206753419735 2 15.549717290605475 19.195803043041064 40.31868684371033 24.935792822643126 3 19.727073070514503 23.197841114358216 29.16238913270775 27.91269668241953 4 12.974954562127275 15.919782063064883 36.84815713879467 34.25710623601317 5 14.413150745460893 36.4248049221729 33.98259663828484 15.179447694081366 6 32.96191965410212 35.07002346139465 17.36369943855081 14.604357445952425 7 29.626238279638663 31.591290555345157 20.201487398486723 18.580983766529453 8 26.606097126690713 34.401523045167075 19.440835679946186 19.551544148196022 9 26.24160133224511 16.963000519377736 20.24588001549889 36.54951813287826 10-11 25.43019590101171 25.899002099270668 27.168684694080508 21.502117305637114 12-13 26.245475728139333 23.108028956988658 27.58906236024271 23.0574329546293 14-15 23.045624875961675 24.899558583287085 25.789522602641906 26.265293938109334 16-17 22.81045673507931 28.059247907453532 26.397473157658492 22.732822199808663 18-19 22.550314077225426 26.74174734552658 28.491681245155814 22.21625733209218 20-21 23.26554911729074 25.844279288258516 28.484109872726027 22.406061721724722 22-23 22.879262343877276 25.68316088648799 28.523634796093873 22.913941973540865 24-25 22.96800997057576 25.92741168658124 28.464312521260517 22.64026582158249 26-27 22.720866002618695 26.075513565801632 28.56302532262368 22.640595108955992 28-29 22.42625516417822 26.373199594200464 28.571019451840975 22.629525789780345 30-31 22.59969560104988 25.95309074958087 28.520661073588354 22.926552575780896 32-33 22.494177945234465 26.091005571824365 28.54952697285912 22.86528951008205 34-35 23.183896100899286 26.115947809805114 28.107837594609563 22.592318494686044 36-37 22.97821314628375 25.903764513666616 28.263448996263605 22.854573343786022 38-39 22.70993986354125 26.187462244737336 28.406091278524027 22.696506613197386 40-41 22.937548688518543 26.050646979112436 28.3458502702245 22.665954062144525 42-43 22.7584662307824 26.15006833313107 27.96235403876311 23.129111397323417 44-45 22.886442201227723 26.1452760517774 27.925932915851625 23.04234883114325 46-47 23.062182167464908 26.395558081069264 27.57128864520154 22.970971106264283 48-49 23.101046358636506 26.444898357794244 27.533493736806168 22.92056154676308 50-51 22.816411847666966 26.585219364801706 27.5898459753845 23.008522812146836 52-53 23.142820526873393 26.68132561870269 27.223042026495726 22.952811827928187 54-55 22.85829728612643 26.421623956032093 27.364249659720137 23.35582909812134 56-57 22.93851306580869 26.69252247411397 27.32593069368945 23.04303376638789 58-59 23.18189394936828 26.388846821820998 27.35508444560378 23.074174783206935 60-61 23.10709649132386 26.43202829802845 27.783264101265008 22.677611109382685 62-63 22.88617639944635 26.5305861754517 27.682927181031474 22.900310244070482 64-65 22.70036673809131 26.682586121641666 27.4246744684358 23.19237267183122 66-67 22.770707658931364 26.775102631089815 27.150863683740045 23.303326026238775 68-69 22.640033160310768 26.524126249102615 27.562158577932195 23.273682012654426 70-71 22.838905068321917 26.487296213353034 27.374941044931184 23.298857673393865 72-73 22.834233546620553 26.396642519025644 27.53467806213326 23.23444587222054 74-75 22.608463213848207 26.839286980292453 27.297578476519284 23.254671329340056 76-77 22.838867180053548 26.627607936853703 27.195064825277477 23.338460057815276 78-79 22.790523458410775 26.64332149789485 27.2922435274774 23.27391151621697 80-81 22.88981487457233 26.87350755601156 27.087741118431158 23.14893645098495 82-83 22.947945709222036 26.768937687138322 27.25412142332106 23.02899518031858 84-85 22.807912307837505 26.776595091405074 27.03865031155248 23.37684228920494 86-87 22.821338539564078 26.675564903031272 27.252178038089315 23.250918519315334 88-89 22.756540902251114 26.626719844834273 27.042268729992756 23.574470522921853 90-91 22.809295614984286 26.623857371051685 27.22885556757871 23.337991446385324 92-93 22.989107888841236 26.38267863130308 27.366148498138347 23.262064981717337 94-95 22.71489897101038 26.422636823762886 27.372397312762196 23.490066892464537 96-97 22.62608041253089 26.40715334892853 27.500403892534912 23.466362346005667 98-99 22.552911061758465 26.492840820126094 27.1816524139944 23.772595704121038 100-101 22.53248168327681 26.41302718629113 27.21670131365805 23.83778981677401 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 121.0 1 103.0 2 124.5 3 168.5 4 152.5 5 152.5 6 263.0 7 548.0 8 967.0 9 1266.0 10 1245.0 11 1178.5 12 1291.0 13 1483.0 14 1667.5 15 1807.0 16 1976.0 17 2166.5 18 2347.0 19 2560.5 20 2880.5 21 3447.5 22 4073.5 23 4967.5 24 6159.5 25 7632.5 26 9809.0 27 12691.5 28 15581.0 29 18958.5 30 23368.0 31 28389.5 32 33761.0 33 38405.0 34 43329.5 35 48423.5 36 54287.5 37 60006.0 38 63461.5 39 65882.5 40 67794.5 41 71740.5 42 76320.5 43 79843.0 44 83589.5 45 87372.5 46 89568.5 47 93071.5 48 101774.0 49 102812.5 50 95677.0 51 91290.0 52 88585.5 53 87635.0 54 86353.0 55 81648.5 56 74641.5 57 67483.5 58 61089.5 59 53711.5 60 44937.0 61 36820.5 62 30232.0 63 24144.0 64 18791.5 65 14696.0 66 11414.5 67 9357.5 68 7225.0 69 5067.5 70 3724.0 71 2821.5 72 2244.5 73 1800.0 74 1387.5 75 1085.0 76 755.0 77 436.5 78 267.0 79 175.5 80 116.0 81 67.5 82 48.0 83 41.0 84 29.5 85 19.0 86 12.0 87 11.0 88 12.5 89 9.5 90 6.0 91 5.5 92 5.0 93 2.5 94 1.0 95 1.5 96 1.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012856351697038425 2 0.012197051610010813 3 0.015617170811466546 4 0.017553864817110156 5 0.030204185236952452 6 0.046521862390885835 7 0.006716619636593792 8 0.02571270339407685 9 0.034448429547192704 10-11 0.04011428967008624 12-13 0.030286597747830907 14-15 0.02680466916321633 16-17 0.029050410084654133 18-19 0.023961437537909754 20-21 0.05358873519871305 22-23 0.013165398612832618 24-25 0.0031110722856615416 26-27 0.0013804095572140615 28-29 8.241251087845144E-5 30-31 0.0027402159867085103 32-33 0.0021633284105593504 34-35 0.0 36-37 3.502531712334186E-4 38-39 8.241251087845144E-5 40-41 0.0015452345789709644 42-43 0.00418243492708141 44-45 0.0017718689838867058 46-47 0.0011125688968590943 48-49 7.829188533452887E-4 50-51 0.0038527848835676042 52-53 0.003502531712334186 54-55 0.005645256995173924 56-57 9.889501305414172E-4 58-59 0.0055834476120150845 60-61 0.003069866030222316 62-63 7.005063424668372E-4 64-65 6.593000870276115E-4 66-67 0.01677094596376487 68-69 0.04221580869748675 70-71 0.05109575674463989 72-73 0.014587014425485904 74-75 0.030492629025027033 76-77 0.026392606608824073 78-79 0.054886732245048656 80-81 0.07798283841873467 82-83 0.046851512434399646 84-85 0.017265421029035576 86-87 0.020294080803818667 88-89 0.02089157150768744 90-91 0.038775086368311396 92-93 0.0251564189456473 94-95 0.01275333605844036 96-97 0.017636277327988607 98-99 0.048994237717239375 100-101 0.06764006830348901 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2426816.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.39335844310916 #Duplication Level Percentage of deduplicated Percentage of total 1 80.15258812737109 48.40683984919217 2 11.827588797723637 14.286156195572522 3 3.518132521695959 6.374155152994307 4 1.4755428339304089 3.5645194907088102 5 0.7729980527987846 2.3341974239251204 6 0.4813955596317075 1.7443856751455302 7 0.30830552352971086 1.3033724194764176 8 0.2334413487980897 1.1278645642724718 9 0.16530245527858287 0.8984853389848928 >10 0.9453157053563394 10.488511196824037 >50 0.07737068528500796 3.23866579113075 >100 0.0393535095351041 4.294910967933617 >500 0.00184491627615744 0.7452393481665703 >1k 7.516325569510865E-4 0.961065351316035 >5k 6.833023245009878E-5 0.23163123435685967 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5613 0.23129071178037397 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4537 0.1869527809277671 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3882 0.15996268361507424 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2710 0.1116689522403017 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2624 0.10812521427252827 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.060312771961286E-5 0.0 16-17 0.0 0.0 0.0 4.120625543922572E-5 0.0 18-19 0.0 0.0 0.0 8.241251087845144E-5 0.0 20-21 0.0 4.120625543922572E-5 0.0 2.472375326353543E-4 0.0 22-23 0.0 4.120625543922572E-5 0.0 4.532688098314829E-4 2.060312771961286E-5 24-25 0.0 6.180938315883858E-5 0.0 6.386969593079986E-4 4.120625543922572E-5 26-27 0.0 8.241251087845144E-5 0.0 9.065376196629658E-4 4.120625543922572E-5 28-29 0.0 8.241251087845144E-5 0.0 0.0016688533452886415 4.120625543922572E-5 30-31 0.0 8.241251087845144E-5 0.0 0.0030080566470634773 4.120625543922572E-5 32-33 0.0 8.241251087845144E-5 0.0 0.004676909992352119 1.2361876631767716E-4 34-35 0.0 8.241251087845144E-5 0.0 0.005995510166407342 1.2361876631767716E-4 36-37 0.0 8.241251087845144E-5 0.0 0.008921154302592368 1.2361876631767716E-4 38-39 0.0 8.241251087845144E-5 0.0 0.012197051610010813 1.2361876631767716E-4 40-41 0.0 8.241251087845144E-5 0.0 0.016173455259896094 1.2361876631767716E-4 42-43 0.0 8.241251087845144E-5 0.0 0.020438302697855956 1.2361876631767716E-4 44-45 0.0 8.241251087845144E-5 0.0 0.022519218597536854 1.2361876631767716E-4 46-47 0.0 8.241251087845144E-5 0.0 0.026021750309871042 1.2361876631767716E-4 48-49 0.0 8.241251087845144E-5 0.0 0.03098710409029774 1.2361876631767716E-4 50-51 0.0 8.241251087845144E-5 0.0 0.03654994857459321 1.2361876631767716E-4 52-53 0.0 8.241251087845144E-5 0.0 0.046521862390885835 1.2361876631767716E-4 54-55 0.0 8.241251087845144E-5 0.0 0.053712353965030726 1.2361876631767716E-4 56-57 0.0 8.241251087845144E-5 0.0 0.07270843772251379 1.2361876631767716E-4 58-59 0.0 8.241251087845144E-5 0.0 0.10604429837284737 1.2361876631767716E-4 60-61 0.0 8.241251087845144E-5 0.0 0.1320454455549988 1.2361876631767716E-4 62-63 0.0 8.241251087845144E-5 0.0 0.14683849125768084 1.2361876631767716E-4 64-65 0.0 8.241251087845144E-5 0.0 0.17617734513040956 1.2361876631767716E-4 66-67 0.0 8.241251087845144E-5 0.0 0.2380897439278462 1.2361876631767716E-4 68-69 0.0 8.241251087845144E-5 0.0 0.31990476410242885 1.2361876631767716E-4 70-71 0.0 8.241251087845144E-5 0.0 0.39273682059126036 1.2361876631767716E-4 72-73 0.0 8.241251087845144E-5 0.0 0.47595285345077665 1.2361876631767716E-4 74-75 0.0 8.241251087845144E-5 0.0 0.5737971069912182 1.2361876631767716E-4 76-77 0.0 8.241251087845144E-5 0.0 0.6394180687781851 1.2361876631767716E-4 78-79 0.0 1.2361876631767716E-4 0.0 0.7250652707086157 1.2361876631767716E-4 80-81 0.0 1.2361876631767716E-4 0.0 0.8439247145230623 1.4422189403729001E-4 82-83 0.0 1.2361876631767716E-4 0.0 0.9524413882222633 1.6482502175690287E-4 84-85 0.0 1.2361876631767716E-4 0.0 1.0957361415121707 1.6482502175690287E-4 86-87 0.0 1.2361876631767716E-4 0.0 1.3096790197526307 1.6482502175690287E-4 88-89 0.0 1.2361876631767716E-4 0.0 1.588089084627759 1.6482502175690287E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11645 0.0 26.14931 1 GTACATG 8500 0.0 25.876394 1 TACATGG 8825 0.0 24.654797 2 ACATGGG 8970 0.0 23.780579 3 GGTATCA 8495 0.0 23.207394 1 ATCAACG 13770 0.0 21.873781 3 TCAACGC 13840 0.0 21.797924 4 TATCAAC 14080 0.0 21.492523 2 CAACGCA 13920 0.0 21.467867 5 GAGTACT 6635 0.0 21.231426 12-13 AACGCAG 14135 0.0 21.108156 6 AGTACTT 7065 0.0 20.746191 12-13 AGAGTAC 11260 0.0 20.444134 10-11 CATGGGG 5730 0.0 19.982044 4 GTACTTT 7055 0.0 19.531345 14-15 ACGCAGA 15645 0.0 19.102026 7 CGCAGAG 15810 0.0 18.962772 8 GTACCTG 2170 0.0 17.951296 1 TACCTGG 2150 0.0 17.676739 2 GCAGAGT 16770 0.0 17.56741 9 >>END_MODULE