FastQCFastQC Report
Thu 26 May 2016
SRR936627_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936627_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2426816
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66030.2720849046652074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58930.24282846330335714No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51430.21192377172393784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33380.13754648065613545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG88350.023.4974521
GTATCAA143950.022.309041
ACATGGG89750.022.2808063
TACATGG91050.022.2770672
GGTATCA111500.021.643861
GAGTACT81150.021.3354212-13
GTACTTT84450.020.53025614-15
CATGGGG55700.019.356914
AGTACTT85800.019.18262912-13
CAACGCA167400.019.0952325
ATCAACG167250.019.0847453
TCAACGC167500.019.0554754
TACCTGG22550.018.9585912
AGAGTAC130200.018.87915810-11
AACGCAG170800.018.769966
GTACCTG23050.018.7549691
TATCAAC170550.018.5495172
TATACCG1559.632933E-418.3858995
ACTTTTT99450.017.43314716-17
GTAAGGT7650.017.384494