##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936627_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2426816 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.970118871805692 31.0 31.0 34.0 30.0 34.0 2 32.1039576135974 33.0 31.0 34.0 30.0 34.0 3 32.22906310161133 34.0 31.0 34.0 30.0 34.0 4 35.93903616920277 37.0 35.0 37.0 35.0 37.0 5 35.80304604881458 37.0 35.0 37.0 35.0 37.0 6 35.853280182757985 37.0 35.0 37.0 35.0 37.0 7 35.77942126638361 37.0 35.0 37.0 33.0 37.0 8 35.74680569107835 37.0 35.0 37.0 33.0 37.0 9 37.37706937814816 39.0 37.0 39.0 34.0 39.0 10-11 37.28178135466389 39.0 37.0 39.0 34.0 39.0 12-13 37.155344080474165 39.0 37.0 39.0 33.0 39.0 14-15 38.292393613689704 40.0 38.0 41.0 33.0 41.0 16-17 38.26945903603735 40.0 38.0 41.0 33.0 41.0 18-19 38.38794350292729 40.0 38.0 41.0 33.5 41.0 20-21 38.390177706097205 40.0 38.0 41.0 34.0 41.0 22-23 38.295377358646064 40.0 38.0 41.0 34.0 41.0 24-25 38.18770644333975 40.0 38.0 41.0 33.0 41.0 26-27 38.00108578483082 40.0 37.0 41.0 33.0 41.0 28-29 38.021077411719716 40.0 37.0 41.0 33.0 41.0 30-31 37.917026259922466 40.0 37.0 41.0 33.0 41.0 32-33 37.757136717410795 40.0 37.0 41.0 33.0 41.0 34-35 37.59305031778264 40.0 37.0 41.0 32.5 41.0 36-37 37.43448844082123 40.0 36.0 41.0 32.0 41.0 38-39 37.2526219540336 39.0 36.0 41.0 31.5 41.0 40-41 37.044680148804034 39.0 36.0 41.0 31.0 41.0 42-43 36.80046983372452 39.0 35.5 41.0 31.0 41.0 44-45 36.55553325839289 39.0 35.0 40.0 30.0 41.0 46-47 36.22338611579947 39.0 35.0 40.0 30.0 41.0 48-49 35.87280968149213 39.0 35.0 40.0 29.0 41.0 50-51 35.58534454198423 38.0 35.0 40.0 28.0 41.0 52-53 35.31491612878768 38.0 34.0 40.0 27.5 41.0 54-55 34.984798394274634 38.0 34.0 40.0 27.0 41.0 56-57 34.638445395118545 38.0 34.0 40.0 26.0 41.0 58-59 34.267628654170736 37.0 33.0 40.0 26.0 41.0 60-61 33.883354980352856 37.0 33.0 39.5 25.0 40.5 62-63 33.55399132031435 36.0 32.0 39.0 24.0 40.0 64-65 33.236219185962184 36.0 32.0 39.0 23.5 40.0 66-67 32.778211450723916 35.5 31.5 39.0 22.0 40.0 68-69 32.31578207824573 35.0 31.0 38.0 21.5 40.0 70-71 31.814146189904797 35.0 31.0 37.5 20.5 39.5 72-73 31.247524534204487 34.0 30.0 37.0 18.5 39.0 74-75 30.70809303218703 34.0 29.5 36.0 18.0 39.0 76-77 29.325265491903796 32.5 28.0 35.0 16.0 37.0 78-79 30.106161118107018 34.0 29.0 35.0 16.5 37.0 80-81 29.9504651362114 34.0 29.0 35.0 12.5 37.0 82-83 29.549151645613016 34.0 29.0 35.0 8.5 36.5 84-85 29.099989657229884 34.0 29.0 35.0 7.0 36.0 86-87 28.67869628352541 33.0 29.0 35.0 2.0 35.5 88-89 28.187117605949524 33.0 27.5 35.0 2.0 35.0 90-91 27.614120724438937 33.0 26.5 34.5 2.0 35.0 92-93 27.018118596547907 32.0 25.5 34.0 2.0 35.0 94-95 26.264216776220366 32.0 25.0 34.0 2.0 35.0 96-97 25.42536022508505 32.0 23.0 34.0 2.0 35.0 98-99 24.27978058493104 31.0 10.0 34.0 2.0 35.0 100-101 20.445130162319682 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18219859833086716 1101 2 0.29507643899797387 1101 3 0.20579912485923302 1101 4 0.13435209342640775 1101 5 0.1367556046146703 1101 6 0.11657186513902218 1101 7 0.10603597176423563 1101 8 0.1328283374636854 1101 9 0.19227987963704152 1101 10-11 0.15309841126862267 1101 12-13 0.18613134528845876 1101 14-15 0.22626274759372222 1101 16-17 0.19924474096592348 1101 18-19 0.1806705684717116 1101 20-21 0.11935824663283512 1101 22-23 0.15951658956658576 1101 24-25 0.17860789403435007 1101 26-27 0.09394322329659843 1101 28-29 0.11602135454675278 1101 30-31 0.235964175816342 1101 32-33 0.2345915034465733 1101 34-35 0.28824051595530875 1101 36-37 0.22476280536922388 1101 38-39 0.19355134827892329 1101 40-41 0.34045234811807035 1101 42-43 0.29692110820130324 1101 44-45 0.3142249603983913 1101 46-47 0.36687730476128877 1101 48-49 0.40540270424049396 1101 50-51 0.3870029882829442 1101 52-53 0.4292051935781984 1101 54-55 0.3790019655056369 1101 56-57 0.44972305589494965 1101 58-59 0.24597481140000355 1101 60-61 0.17738540338651632 1101 62-63 0.24271005566887283 1101 64-65 0.19306634690423152 1101 66-67 0.2362125393610981 1101 68-69 0.10782004639826681 1101 70-71 -0.09534790938761262 1101 72-73 0.20644787870874737 1101 74-75 0.19816729756055196 1101 76-77 0.15285678900348998 1101 78-79 0.2534497641702842 1101 80-81 0.3560697212395354 1101 82-83 0.2717478858708766 1101 84-85 0.3435025282314399 1101 86-87 0.25131381393606844 1101 88-89 -0.10462576063583384 1101 90-91 0.22206639841822806 1101 92-93 0.3660457072834653 1101 94-95 0.14689964717846848 1101 96-97 0.3535875581845751 1101 98-99 -0.2454881307057022 1101 100-101 0.22447134251726553 1102 1 0.23672421917836672 1102 2 0.328190115695449 1102 3 0.18980899229617165 1102 4 0.09192638302992862 1102 5 0.05613600797280327 1102 6 0.0943902114037698 1102 7 0.2093589071777089 1102 8 0.011974713747989085 1102 9 0.1335761059384808 1102 10-11 0.29869545144165244 1102 12-13 0.33436440400574696 1102 14-15 0.34960855076957387 1102 16-17 0.3965258539344134 1102 18-19 0.30791145574796985 1102 20-21 0.19407747311953472 1102 22-23 0.24617067147101324 1102 24-25 0.20312841673976578 1102 26-27 0.261511588851441 1102 28-29 0.3731610581624665 1102 30-31 0.47726636278921575 1102 32-33 0.43388358531337445 1102 34-35 0.3747911701074287 1102 36-37 0.28334768135253086 1102 38-39 0.3348770369669438 1102 40-41 0.36563814237312187 1102 42-43 0.337184812346905 1102 44-45 0.4233859441162906 1102 46-47 0.27858845820046696 1102 48-49 0.4378506042466981 1102 50-51 0.2838014762929646 1102 52-53 0.41180584210430027 1102 54-55 0.25441231416576215 1102 56-57 0.31052395039978364 1102 58-59 0.241829113899783 1102 60-61 0.15976915671777192 1102 62-63 0.07861100294837797 1102 64-65 0.13649812266275774 1102 66-67 0.2090229113335127 1102 68-69 0.14074056955831793 1102 70-71 -0.07641962043244632 1102 72-73 -0.17573679829926547 1102 74-75 0.1925157096667256 1102 76-77 0.10439886648186203 1102 78-79 0.03293567141289344 1102 80-81 0.17744797244167643 1102 82-83 0.30588611611468863 1102 84-85 0.16569803547637107 1102 86-87 0.4137646526038061 1102 88-89 0.22717204732724383 1102 90-91 0.04341434112152598 1102 92-93 0.0731528987199006 1102 94-95 -0.022163306225625234 1102 96-97 0.2703822045923694 1102 98-99 0.3293119184445814 1102 100-101 0.13355664338783768 1103 1 0.18061543220028042 1103 2 0.261123441787376 1103 3 0.22700829208423556 1103 4 0.10215279447172065 1103 5 0.14949611586130374 1103 6 0.15280713360777298 1103 7 0.1832913644278591 1103 8 0.14909679587496782 1103 9 0.13526011488713152 1103 10-11 0.2167857140669227 1103 12-13 0.0942568436577389 1103 14-15 0.22754505727265695 1103 16-17 0.1699256606803985 1103 18-19 0.17443746611835564 1103 20-21 0.21535430294979818 1103 22-23 0.23317893672429335 1103 24-25 0.1381823985115176 1103 26-27 0.1230753692276707 1103 28-29 0.27441015017765125 1103 30-31 0.3152177684470061 1103 32-33 0.21537000557459152 1103 34-35 0.23446762670839405 1103 36-37 0.2542351858662144 1103 38-39 0.18286858283501317 1103 40-41 0.29904054890331366 1103 42-43 0.3141291526713843 1103 44-45 0.30581706287185995 1103 46-47 0.252776378590589 1103 48-49 0.24264140619999353 1103 50-51 0.1895666758410286 1103 52-53 0.1830945960915784 1103 54-55 0.2529483456613093 1103 56-57 0.3037225384269604 1103 58-59 0.07741025152414238 1103 60-61 -0.01880663723076026 1103 62-63 0.2443918266398839 1103 64-65 0.2262078486157435 1103 66-67 0.10544707604790204 1103 68-69 0.11835934020380279 1103 70-71 -0.16050305213742533 1103 72-73 0.1420008399748518 1103 74-75 0.20941278072490732 1103 76-77 0.12836979265244253 1103 78-79 0.024786607112623926 1103 80-81 0.15046535398328587 1103 82-83 -0.0616946892867567 1103 84-85 0.045891101251548605 1103 86-87 0.2570872709426375 1103 88-89 0.16028064262847508 1103 90-91 0.21067891151927043 1103 92-93 0.3062858129419048 1103 94-95 0.20325566413518104 1103 96-97 0.4814512310253285 1103 98-99 0.3700648714064201 1103 100-101 0.22216839296248025 1104 1 0.22202660954436126 1104 2 0.23118169420943957 1104 3 0.1873759041750489 1104 4 0.16348222313889238 1104 5 0.08881236970624684 1104 6 0.1077018616627683 1104 7 0.16588429098911206 1104 8 0.060590194957228505 1104 9 0.14423734192847348 1104 10-11 0.16733770014020877 1104 12-13 0.17898429118648806 1104 14-15 0.17357476209428313 1104 16-17 0.18763429420668132 1104 18-19 0.20592226312830064 1104 20-21 0.20804814178137576 1104 22-23 0.1797866591662043 1104 24-25 0.09342395031587358 1104 26-27 0.14410788408594755 1104 28-29 0.1817633407519068 1104 30-31 0.283802612908012 1104 32-33 0.255290315175408 1104 34-35 0.24245610298859077 1104 36-37 0.2568735236738675 1104 38-39 0.3483126035448123 1104 40-41 0.3752965101012222 1104 42-43 0.3606367416151386 1104 44-45 0.4313957228803531 1104 46-47 0.28488504985636354 1104 48-49 0.29763293392303325 1104 50-51 0.231707342651859 1104 52-53 0.30903951621077397 1104 54-55 0.17221049131174482 1104 56-57 0.31816849868796737 1104 58-59 0.4007866993189353 1104 60-61 0.23247902865700354 1104 62-63 0.25165456895366134 1104 64-65 0.3519782367987574 1104 66-67 0.48259201029304677 1104 68-69 0.3083103065467441 1104 70-71 0.23226772833065468 1104 72-73 0.36268886344712215 1104 74-75 0.41433784926937633 1104 76-77 0.20195836032965886 1104 78-79 0.3909129034026222 1104 80-81 0.4013859457078226 1104 82-83 0.3122940434216339 1104 84-85 0.44616023226435075 1104 86-87 0.4105382001917768 1104 88-89 0.22482128761786768 1104 90-91 0.29099595217447316 1104 92-93 0.3716871922515246 1104 94-95 0.45949740450518917 1104 96-97 0.5484026436798395 1104 98-99 0.4466545450455328 1104 100-101 0.5017367318019019 1105 1 0.11107167390001749 1105 2 0.26762450709247787 1105 3 0.1922217236753525 1105 4 0.15387394159600376 1105 5 0.17284736341337492 1105 6 0.20415958033751025 1105 7 0.20409362497124306 1105 8 0.16735754757775112 1105 9 0.1982484576249277 1105 10-11 0.23647573064575766 1105 12-13 0.2113918639856749 1105 14-15 0.3265880520653184 1105 16-17 0.34994132477176976 1105 18-19 0.31579847935671523 1105 20-21 0.23512904984385585 1105 22-23 0.2274326322413387 1105 24-25 0.2631033936687359 1105 26-27 0.3169403335427958 1105 28-29 0.25491423212235276 1105 30-31 0.25914379333995186 1105 32-33 0.3266681154008353 1105 34-35 0.06629264024588366 1105 36-37 0.26972761321948724 1105 38-39 0.20951997267258093 1105 40-41 0.31435009498518696 1105 42-43 0.31671926960455465 1105 44-45 0.3066317825774334 1105 46-47 0.24966672928329103 1105 48-49 0.18579904932177982 1105 50-51 0.4640498309335612 1105 52-53 0.4682758715599107 1105 54-55 0.46630402002180915 1105 56-57 0.6247652354282849 1105 58-59 0.6147328536862702 1105 60-61 0.4589446108554611 1105 62-63 0.47815916079981946 1105 64-65 0.41119529263798427 1105 66-67 0.537750625573743 1105 68-69 0.42135574868003545 1105 70-71 0.3645590934424803 1105 72-73 0.33814050277623764 1105 74-75 0.49297349710670346 1105 76-77 0.4369818379781556 1105 78-79 0.3327057868109975 1105 80-81 0.6247588840992719 1105 82-83 0.48023119271977066 1105 84-85 0.4933210485104347 1105 86-87 0.6109165937744869 1105 88-89 0.6242414810646579 1105 90-91 0.629697628973382 1105 92-93 0.7681849471436415 1105 94-95 0.7120754289334421 1105 96-97 0.8623373004667947 1105 98-99 0.6889697471052436 1105 100-101 0.4700124437793036 1106 1 0.11378982869109322 1106 2 0.2350850579108652 1106 3 0.22157256819820503 1106 4 0.17746830926564172 1106 5 0.13913614557984033 1106 6 0.1339340475421409 1106 7 0.1169819911380543 1106 8 0.16399688023590642 1106 9 0.24719484594209007 1106 10-11 0.18043230115333841 1106 12-13 0.16679278092731664 1106 14-15 0.19422828859705277 1106 16-17 0.2193207012078915 1106 18-19 0.1607928709844515 1106 20-21 0.12432612695624812 1106 22-23 0.055041069846836876 1106 24-25 0.15868203611594822 1106 26-27 0.09930173051753854 1106 28-29 0.21423011960642668 1106 30-31 0.21022027411358835 1106 32-33 0.20713464485296385 1106 34-35 0.12829292338700782 1106 36-37 0.13486858482914243 1106 38-39 0.18950080619649157 1106 40-41 0.04939207610185292 1106 42-43 0.14920862458729545 1106 44-45 0.167992820180757 1106 46-47 0.18770565029780784 1106 48-49 0.237455969399754 1106 50-51 0.21116342497452933 1106 52-53 0.18709604427600368 1106 54-55 0.14840732297192005 1106 56-57 0.19351081134821868 1106 58-59 0.11823835860312215 1106 60-61 0.08768125693994477 1106 62-63 0.268372278945904 1106 64-65 0.33780946929626054 1106 66-67 0.06054021904842699 1106 68-69 0.15819349792590032 1106 70-71 0.032349612570840236 1106 72-73 0.024435383366917307 1106 74-75 -0.023377902808356055 1106 76-77 -0.15821129693859248 1106 78-79 -0.12146128823228963 1106 80-81 -0.11494512452564365 1106 82-83 0.014808168990366966 1106 84-85 -0.011065460904010394 1106 86-87 -0.10749846933331852 1106 88-89 0.057538877255318255 1106 90-91 0.13626977006478214 1106 92-93 0.10843236892590369 1106 94-95 0.12372559718460607 1106 96-97 0.25599058327032864 1106 98-99 0.6463962120883124 1106 100-101 0.48518880802946995 1107 1 0.05793863530193377 1107 2 0.10177451751708588 1107 3 0.01293046874501158 1107 4 0.05467425241290158 1107 5 0.01788649659855679 1107 6 0.08102642077131605 1107 7 0.022607154543315744 1107 8 -9.973358694637113E-4 1107 9 -0.10335251979743987 1107 10-11 0.05126016902281805 1107 12-13 0.10363816360661815 1107 14-15 0.13395593531542715 1107 16-17 0.13401708212111174 1107 18-19 0.05931497976338562 1107 20-21 0.09354120813561195 1107 22-23 0.13546811717048968 1107 24-25 0.1447262022779654 1107 26-27 0.1260394128331157 1107 28-29 0.1666766735150773 1107 30-31 0.2800480086518107 1107 32-33 0.1423698478777169 1107 34-35 0.1919329707307753 1107 36-37 0.21288099580819164 1107 38-39 0.2649213768989256 1107 40-41 0.241187511703977 1107 42-43 0.16433993352026732 1107 44-45 0.12312201369057618 1107 46-47 0.1173229151301669 1107 48-49 0.13947374044393257 1107 50-51 -0.015954139666916944 1107 52-53 -0.02997080235060423 1107 54-55 0.19053806059167755 1107 56-57 0.035020627581999975 1107 58-59 0.07478071436516132 1107 60-61 0.0589581308212459 1107 62-63 -0.12762681529545716 1107 64-65 -0.06327915427088726 1107 66-67 -0.05296087329947596 1107 68-69 0.03941472277080749 1107 70-71 -0.12648656205883313 1107 72-73 -0.00965101081239439 1107 74-75 -0.017770392758233555 1107 76-77 -0.2090937784789162 1107 78-79 -0.050684294799786755 1107 80-81 -0.03343489116616638 1107 82-83 0.03830314597768947 1107 84-85 0.001749608655419621 1107 86-87 0.18986209336924276 1107 88-89 -0.1588443301581961 1107 90-91 -0.024281759482985166 1107 92-93 -0.10500332407256607 1107 94-95 0.15966179483067933 1107 96-97 0.06049360407968862 1107 98-99 0.26523494906151157 1107 100-101 6.042838395323713E-4 1108 1 0.05735358536032109 1108 2 0.1322123382631446 1108 3 -0.03648059924896785 1108 4 0.05661604580486568 1108 5 0.09654549453878047 1108 6 0.05161148593671072 1108 7 0.03356827757126979 1108 8 0.09128344547591638 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-0.9648205849243041 2316 100-101 -0.8073051443883905 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 11.0 4 22.0 5 61.0 6 152.0 7 372.0 8 817.0 9 1564.0 10 2535.0 11 3840.0 12 5614.0 13 7861.0 14 10121.0 15 13377.0 16 16893.0 17 21436.0 18 20019.0 19 14897.0 20 14668.0 21 15477.0 22 16992.0 23 19036.0 24 22285.0 25 26731.0 26 32712.0 27 40434.0 28 49554.0 29 61510.0 30 75383.0 31 94516.0 32 117513.0 33 151212.0 34 199620.0 35 269676.0 36 380706.0 37 470756.0 38 239176.0 39 9261.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.76069743779103 19.168865614770667 14.805614086565269 23.26482286087304 2 15.86588587276796 20.213468282651785 39.33964946694745 24.58099637763281 3 19.943931580539324 23.509917673461413 29.362445285283005 27.183705460716258 4 13.560957720129965 16.69260612204112 36.20274393698711 33.543692220841805 5 14.311605027607666 36.85939767114013 33.823728788415124 15.00526851283708 6 33.02203380890846 35.78091622933094 16.70377976739893 14.493270194361665 7 29.58461553676181 32.041089297399225 19.86701071199582 18.50728445384315 8 26.774343355841978 34.9645269372347 18.869559591737335 19.39157011518598 9 26.17294684596889 17.01798447759718 19.941528299437742 36.86754037699619 10-11 25.566050330968643 26.321072549381576 26.717538536090085 21.395338583559692 12-13 26.35968281072813 23.413847609377886 27.395875913130617 22.830593666763363 14-15 23.035959169411015 25.26299979231979 25.551011864144176 26.150029174125024 16-17 22.711667097632915 28.431018443638372 26.263316599480575 22.59399785924814 18-19 22.5523313156337 27.009731233673023 28.489020503973695 21.948916946719585 20-21 23.204831043285306 25.978972352537593 28.719447998500087 22.09674860567701 22-23 22.867008253844503 25.889887891854674 28.633491270530826 22.60961258377 24-25 22.98169815869013 26.20902791942492 28.57145506128428 22.237818860600665 26-27 22.7866753639444 26.323433243517318 28.60939429614066 22.28049709639762 28-29 22.45672721518831 26.644069137560606 28.631118824928485 22.2680848223226 30-31 22.686553203167463 26.19355686766241 28.523311140880526 22.596578788289605 32-33 22.588930591200317 26.341273255631876 28.562168586126813 22.507627567041 34-35 23.128757921213225 26.384869233664794 28.23818125215176 22.24819159297022 36-37 23.051935877196993 26.05399910416309 28.411298753369174 22.482766265270744 38-39 22.759663549037192 26.404267387980347 28.49341498386584 22.342654079116624 40-41 22.939119792814864 26.318301971926168 28.33607011659315 22.406508118665826 42-43 22.83992434450447 26.467226253610736 27.942178762892546 22.750670638992247 44-45 22.99089463807258 26.42065239159979 27.894483953942427 22.6939690163852 46-47 23.199822318626545 26.79341985548142 27.426492160921967 22.580265664970067 48-49 23.28430579716087 26.777886038020565 27.362862213098047 22.574945951720508 50-51 23.07311621835228 26.88837618685393 27.34443055323196 22.69407704156183 52-53 23.364532771772286 27.083638518357354 26.911581125235983 22.640247584634373 54-55 23.063279043371466 26.811258132832155 27.13198722028404 22.99347560351234 56-57 23.109894932919026 27.176355682668156 27.014026115320345 22.699723269092477 58-59 23.406046076173865 26.834727233468108 26.983011120982507 22.776215569375513 60-61 23.21504547543947 26.89208342270185 27.459529712481658 22.433341389377023 62-63 23.051333971206695 26.971370694251807 27.35695854729699 22.62033678724451 64-65 22.873285020262767 27.007099849736775 27.192883955707686 22.926731174292772 66-67 22.99805999350584 27.128011583997996 26.925996251848115 22.94793217064805 68-69 22.889433186485615 26.89118118393243 27.238679120496034 22.98070650908592 70-71 23.11774763084295 26.91556912309562 27.077249128139492 22.88943411792194 72-73 23.14492310196446 26.785488013301727 27.148380397259302 22.921208487474516 74-75 22.92731875017385 27.327960373476685 26.788926743885366 22.9557941324641 76-77 23.055741271657958 27.091026529874306 26.805526224429983 23.047705974037747 78-79 23.054218555545482 27.141111651405637 26.815787955461417 22.988881837587467 80-81 23.144792818749576 27.318354813241008 26.701216105068315 22.835636262941104 82-83 23.133098135802367 27.255476398622108 26.753921750222364 22.85750371535316 84-85 23.026519112996738 27.318341438730698 26.5531498966943 23.101989551578264 86-87 22.99082064879118 27.170389221550717 26.752988651130057 23.085801478528047 88-89 22.932132227168456 27.27841573322158 26.452150457213992 23.337301582395973 90-91 23.038593606572068 27.186007770026983 26.612682390102332 23.162716233298614 92-93 23.12078168196617 26.997729901552148 26.736167913936526 23.145320502545154 94-95 22.95437355816113 26.98073661032992 26.771181291619783 23.293708539889167 96-97 22.85348197266045 26.980127030748957 26.88036660411552 23.28602439247507 98-99 22.743687888963382 27.126758949943607 26.422705098024025 23.706848063068982 100-101 22.534145688683786 27.376775018486505 26.396810081546647 23.69226921128306 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 226.0 1 172.5 2 142.5 3 175.5 4 175.0 5 186.0 6 289.5 7 578.0 8 980.5 9 1286.5 10 1340.5 11 1311.0 12 1442.5 13 1646.0 14 1883.5 15 2101.5 16 2386.0 17 2661.5 18 2847.0 19 3125.5 20 3423.0 21 3890.5 22 4554.0 23 5435.0 24 6643.5 25 8167.5 26 10302.5 27 13039.5 28 15846.0 29 19479.5 30 24246.5 31 28968.5 32 34123.0 33 38676.0 34 43411.0 35 48565.0 36 54377.5 37 60095.5 38 63626.0 39 65927.5 40 67769.5 41 71540.5 42 76218.0 43 80164.5 44 83502.5 45 86673.5 46 88665.5 47 92734.0 48 102259.5 49 102652.0 50 94983.0 51 90912.0 52 88034.0 53 86645.5 54 85375.0 55 80745.0 56 73459.5 57 66762.0 58 61116.0 59 53877.0 60 44965.5 61 36775.5 62 30190.0 63 24013.5 64 18201.0 65 14087.0 66 11118.5 67 9128.5 68 7049.0 69 4876.0 70 3489.0 71 2717.5 72 2241.0 73 1788.5 74 1429.0 75 1126.0 76 761.0 77 434.5 78 258.0 79 164.0 80 100.5 81 58.5 82 43.5 83 26.0 84 14.5 85 10.0 86 8.0 87 5.0 88 2.0 89 1.0 90 1.5 91 1.5 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011414132756665524 2 0.009312613729265012 3 0.005686463250613149 4 4.532688098314829E-4 5 0.005562844484295472 6 0.0 7 1.6482502175690287E-4 8 0.0018542814947651574 9 4.120625543922572E-5 10-11 0.0 12-13 0.0 14-15 3.2965004351380575E-4 16-17 0.004058816160763733 18-19 0.001751265856167093 20-21 4.532688098314829E-4 22-23 3.0904691579419286E-4 24-25 4.532688098314829E-4 26-27 1.2361876631767716E-4 28-29 8.241251087845144E-5 30-31 6.180938315883857E-4 32-33 0.0037909755004087663 34-35 0.0010507595137002558 36-37 0.0014422189403729 38-39 0.005459828845697408 40-41 4.120625543922572E-5 42-43 0.0010713626414198688 44-45 0.004697513120071732 46-47 0.0 48-49 0.002080915899680899 50-51 9.065376196629658E-4 52-53 3.9145942667264435E-4 54-55 6.180938315883858E-5 56-57 0.011166895224030169 58-59 0.0021427252828397376 60-61 0.0027402159867085103 62-63 8.241251087845144E-5 64-65 0.003069866030222316 66-67 8.241251087845145E-4 68-69 0.0016070439621298032 70-71 0.004491481842875603 72-73 0.002616597220390833 74-75 0.006531191487117277 76-77 9.683470028218042E-4 78-79 0.006428175848519213 80-81 0.0021427252828397376 82-83 0.0025341847095123817 84-85 0.0015658377066905774 86-87 4.3266568211187E-4 88-89 0.0021839315382789627 90-91 0.00756134787309792 92-93 0.00201910651652206 94-95 4.3266568211187E-4 96-97 8.241251087845144E-5 98-99 5.356813207099344E-4 100-101 0.0018748846224847704 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2426816.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.47377545612068 #Duplication Level Percentage of deduplicated Percentage of total 1 78.21928635080809 44.9555770006435 2 12.690942674518393 14.587927792035396 3 3.9139873152221933 6.7485488427955485 4 1.7316031678141177 3.9808708658422343 5 0.9136560450154738 2.6255631187673316 6 0.5475766843101875 1.888277963943048 7 0.352956681550325 1.4200027142812612 8 0.2628200463519535 1.2084208263519525 9 0.19039210168434514 0.9848297610742454 >10 1.040985637028764 10.986983459964641 >50 0.0889680947252136 3.5492564629554324 >100 0.04338229731353083 4.629610783246154 >500 0.0023669962645361297 0.9342252363984347 >1k 8.607259143755945E-4 0.77274962740581 >5k 2.1518147859389861E-4 0.7271555442950699 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6603 0.2720849046652074 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5893 0.24282846330335714 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5143 0.21192377172393784 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3338 0.13754648065613545 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.060312771961286E-5 0.0 16-17 0.0 0.0 0.0 4.120625543922572E-5 0.0 18-19 0.0 0.0 0.0 8.241251087845144E-5 0.0 20-21 0.0 0.0 0.0 2.472375326353543E-4 0.0 22-23 0.0 0.0 0.0 4.3266568211187007E-4 0.0 24-25 0.0 0.0 0.0 5.974907038687729E-4 0.0 26-27 0.0 0.0 0.0 9.065376196629658E-4 0.0 28-29 0.0 0.0 0.0 0.0016688533452886415 0.0 30-31 0.0 0.0 0.0 0.0030286597747830905 0.0 32-33 0.0 0.0 0.0 0.004676909992352119 0.0 34-35 0.0 0.0 0.0 0.006036716421846567 0.0 36-37 0.0 0.0 0.0 0.008921154302592368 0.0 38-39 0.0 0.0 0.0 0.01225886099316965 0.0 40-41 0.0 0.0 0.0 0.01621466151533532 0.0 42-43 0.0 0.0 0.0 0.020561921464173634 0.0 44-45 0.0 0.0 0.0 0.022581027980695695 0.0 46-47 0.0 0.0 0.0 0.026021750309871042 0.0 48-49 0.0 0.0 0.0 0.030945897834858516 0.0 50-51 0.0 0.0 0.0 0.036446932935995144 0.0 52-53 0.0 0.0 0.0 0.046274624858250485 0.0 54-55 0.0 0.0 0.0 0.053135466388881565 0.0 56-57 0.0 0.0 0.0 0.07204913763548618 0.0 58-59 0.0 0.0 0.0 0.10544680766897861 0.0 60-61 0.0 0.0 0.0 0.13113890793533584 0.0 62-63 0.0 0.0 0.0 0.14578773174398058 0.0 64-65 0.0 0.0 0.0 0.17506477623355046 0.0 66-67 0.0 0.0 0.0 0.2373068250745009 0.0 68-69 0.0 0.0 0.0 0.3191836546322424 0.0 70-71 0.0 0.0 0.0 0.3917684735884385 0.0 72-73 0.0 0.0 0.0 0.4744282219995253 0.0 74-75 0.0 0.0 0.0 0.5727257443497982 0.0 76-77 0.0 0.0 0.0 0.6385115311585221 0.0 78-79 0.0 0.0 0.0 0.7249210488145785 0.0 80-81 0.0 0.0 0.0 0.843883508267623 0.0 82-83 0.0 0.0 0.0 0.9522147538173475 0.0 84-85 0.0 0.0 0.0 1.0947265882539097 0.0 86-87 0.0 0.0 0.0 1.3075568975975105 0.0 88-89 0.0 0.0 0.0 1.5828146839315382 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8835 0.0 23.497452 1 GTATCAA 14395 0.0 22.30904 1 ACATGGG 8975 0.0 22.280806 3 TACATGG 9105 0.0 22.277067 2 GGTATCA 11150 0.0 21.64386 1 GAGTACT 8115 0.0 21.33542 12-13 GTACTTT 8445 0.0 20.530256 14-15 CATGGGG 5570 0.0 19.35691 4 AGTACTT 8580 0.0 19.182629 12-13 CAACGCA 16740 0.0 19.095232 5 ATCAACG 16725 0.0 19.084745 3 TCAACGC 16750 0.0 19.055475 4 TACCTGG 2255 0.0 18.958591 2 AGAGTAC 13020 0.0 18.879158 10-11 AACGCAG 17080 0.0 18.76996 6 GTACCTG 2305 0.0 18.754969 1 TATCAAC 17055 0.0 18.549517 2 TATACCG 155 9.632933E-4 18.385899 5 ACTTTTT 9945 0.0 17.433147 16-17 GTAAGGT 765 0.0 17.38449 4 >>END_MODULE