FastQCFastQC Report
Thu 26 May 2016
SRR936626_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936626_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502387
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58400.23337717147667408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47450.18961895182479768No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39850.15924795005728531No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27530.1100149577183705No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27370.1093755682074755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG86250.027.5490931
TACATGG90250.025.2212942
ACATGGG89350.024.6775743
GGTATCA95750.023.9720271
GTATCAA126950.023.9201871
GTATAGG7400.022.476711
GAGTACT67550.021.45237412-13
TCAACGC145350.020.5647474
ATCAACG146150.020.5167983
TATCAAC147900.020.4989472
CAACGCA147550.020.1933025
CATGGGG55300.020.1084444
GTACTTT72000.019.95993214-15
AACGCAG149500.019.864756
CATGGGA36450.018.5130754
GTACCTG20500.018.3134291
AGAGTAC113650.018.22839210-11
ACGCAGA163800.018.1015177
AGTACTT73100.018.06875812-13
CGCAGAG166000.017.7184988
Produced by FastQC (version 0.11.5)