##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936626_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2502387 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.188206300624163 31.0 31.0 34.0 28.0 34.0 2 31.24726231394265 31.0 31.0 34.0 28.0 34.0 3 31.29138618447107 31.0 31.0 34.0 28.0 34.0 4 34.926719967774766 37.0 35.0 37.0 32.0 37.0 5 34.832201813708274 37.0 35.0 37.0 32.0 37.0 6 34.94120853409165 37.0 35.0 37.0 32.0 37.0 7 34.761862573614714 37.0 35.0 37.0 32.0 37.0 8 34.74759659477131 37.0 35.0 37.0 32.0 37.0 9 36.23436063246812 39.0 35.0 39.0 32.0 39.0 10-11 36.09456930522737 39.0 35.0 39.0 31.0 39.0 12-13 35.99143138131711 39.0 35.0 39.0 30.0 39.0 14-15 37.0524029656484 39.0 36.0 41.0 31.0 41.0 16-17 36.98125449820512 39.0 36.0 41.0 31.0 41.0 18-19 36.918143956150665 39.0 36.0 41.0 31.0 41.0 20-21 36.80953085993494 39.0 36.0 41.0 30.0 41.0 22-23 36.70197675259662 39.0 36.0 41.0 30.0 41.0 24-25 36.545583876514705 39.0 36.0 41.0 30.0 41.0 26-27 36.34024932993977 39.0 35.5 40.0 29.5 41.0 28-29 36.454977787208776 39.0 36.0 41.0 30.0 41.0 30-31 36.41570588402193 39.0 35.5 41.0 30.0 41.0 32-33 36.28683592905494 39.0 35.0 40.0 30.0 41.0 34-35 36.12311544936894 39.0 35.0 40.0 29.0 41.0 36-37 35.89348490061689 38.0 35.0 40.0 28.0 41.0 38-39 35.72086272027468 38.0 35.0 40.0 27.0 41.0 40-41 35.579893717478555 38.0 35.0 40.0 27.0 41.0 42-43 35.33280443832229 38.0 34.0 40.0 26.5 41.0 44-45 35.08609719439879 38.0 34.0 40.0 25.5 41.0 46-47 34.789043421341304 38.0 33.5 40.0 24.5 41.0 48-49 34.572079578418524 38.0 33.0 40.0 24.0 41.0 50-51 33.88695033981554 37.5 32.5 39.5 23.5 40.5 52-53 34.083607371681516 37.5 33.0 39.5 24.0 40.5 54-55 34.4390723736976 38.0 34.0 40.0 24.0 41.0 56-57 34.35929214785723 38.0 33.5 40.0 23.5 41.0 58-59 34.12209222634229 38.0 33.0 40.0 23.0 41.0 60-61 33.799684860894814 37.0 33.0 40.0 22.5 41.0 62-63 33.46502619299093 37.0 32.5 40.0 22.0 41.0 64-65 33.14345602818429 36.0 32.0 39.0 21.5 41.0 66-67 32.77152055217678 36.0 32.0 39.0 20.0 41.0 68-69 32.38750421097936 35.0 31.0 39.0 19.5 40.0 70-71 31.987633607431626 35.0 31.0 38.0 18.0 40.0 72-73 31.571407819813643 35.0 31.0 37.5 17.5 39.5 74-75 31.134422453441452 35.0 30.5 37.0 14.0 39.0 76-77 30.715669079163213 35.0 30.0 36.0 11.0 39.0 78-79 30.265908510554123 34.0 30.0 36.0 7.5 38.5 80-81 29.84958721412795 34.0 29.0 35.5 7.0 37.0 82-83 29.441493861660888 34.0 29.0 35.0 3.5 37.0 84-85 29.049833019433045 34.0 29.0 35.0 2.0 36.0 86-87 28.708262550916388 34.0 29.0 35.0 2.0 36.0 88-89 28.33417133321105 34.0 27.5 35.0 2.0 36.0 90-91 27.976132788413622 34.0 27.0 35.0 2.0 35.0 92-93 27.600297036389655 33.5 26.5 35.0 2.0 35.0 94-95 27.170205088181802 33.0 26.0 35.0 2.0 35.0 96-97 26.565113429697327 33.0 25.0 35.0 2.0 35.0 98-99 25.87832577455046 33.0 23.5 35.0 2.0 35.0 100-101 23.748582653282647 31.0 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25205221115030696 1101 2 0.23212933698167149 1101 3 0.26994461544331827 1101 4 0.22349819914691693 1101 5 0.3065263980776507 1101 6 0.12806756181957013 1101 7 0.14676349145220513 1101 8 0.3201580610733501 1101 9 0.32481881248020983 1101 10-11 0.20580681204763351 1101 12-13 0.31908594002207025 1101 14-15 0.4554320749108314 1101 16-17 0.457814607151505 1101 18-19 0.5047054584606698 1101 20-21 0.43367746008580355 1101 22-23 0.4451349578021464 1101 24-25 0.3648839043656835 1101 26-27 0.4456469193673058 1101 28-29 0.43813338761156473 1101 30-31 0.33578564107042297 1101 32-33 0.1796108884425749 1101 34-35 0.10811261884545331 1101 36-37 0.20148350256162928 1101 38-39 0.24765995972246202 1101 40-41 0.15553262313734706 1101 42-43 0.06080055124579786 1101 44-45 0.15238775366726998 1101 46-47 0.3735430617756492 1101 48-49 0.3159165513426885 1101 50-51 0.4040302268395308 1101 52-53 0.4191694956132963 1101 54-55 0.5023557542033146 1101 56-57 0.400297340449832 1101 58-59 0.4623595922593893 1101 60-61 0.3661050651143398 1101 62-63 0.3893517017158885 1101 64-65 0.49278664218196866 1101 66-67 0.4087546837919831 1101 68-69 0.4330980162947853 1101 70-71 0.3008881278860507 1101 72-73 0.255234412629715 1101 74-75 0.19579665688484837 1101 76-77 0.21712003083793618 1101 78-79 0.21652333534643375 1101 80-81 0.04637607643958219 1101 82-83 0.23004763531597305 1101 84-85 0.11672555991019706 1101 86-87 0.1555619807011155 1101 88-89 0.1003481563190185 1101 90-91 0.27330352208632647 1101 92-93 0.13471693401872287 1101 94-95 -0.05760661863677541 1101 96-97 -0.022817913766925102 1101 98-99 0.032060862015537595 1101 100-101 0.1834301025398979 1102 1 0.12656798863842766 1102 2 0.18133061786278049 1102 3 0.23883990127578514 1102 4 0.3490053163402109 1102 5 0.2675642292492171 1102 6 0.2869337561435188 1102 7 0.031926822109404895 1102 8 0.060944478116759626 1102 9 0.3662230806593456 1102 10-11 0.36106556773696497 1102 12-13 0.4315285888451257 1102 14-15 0.32875122371710574 1102 16-17 0.23181662798871372 1102 18-19 0.12378756489346188 1102 20-21 0.0749941179507374 1102 22-23 -0.25630237029695024 1102 24-25 -0.046806730488569315 1102 26-27 0.0066459822116797795 1102 28-29 0.034133076243897165 1102 30-31 0.03250437533034045 1102 32-33 0.001529951164847887 1102 34-35 -0.050062156099834 1102 36-37 -0.2069919558662079 1102 38-39 -0.15438590900859595 1102 40-41 -0.48592903437653945 1102 42-43 -0.13039140692104212 1102 44-45 -0.04960057309784105 1102 46-47 -0.011762215906308882 1102 48-49 0.09377174706168745 1102 50-51 -0.12427370463515786 1102 52-53 -0.14646497706114303 1102 54-55 -0.2613955546093152 1102 56-57 -0.25622596480953064 1102 58-59 -0.11184207019974224 1102 60-61 -0.4713327758256227 1102 62-63 -0.34371844453533384 1102 64-65 -0.07259141006064596 1102 66-67 -0.1386457014491853 1102 68-69 0.028929047440712452 1102 70-71 -0.055391272700671124 1102 72-73 -0.2490659716956678 1102 74-75 -0.2485478989125447 1102 76-77 -0.26981244816763805 1102 78-79 -0.3208392584072861 1102 80-81 -0.2733840178254532 1102 82-83 -0.2699981540468528 1102 84-85 -0.25302965015613665 1102 86-87 -0.25861806917876606 1102 88-89 -0.5730218186799902 1102 90-91 -0.6309226834712796 1102 92-93 -0.5894166549210063 1102 94-95 -0.5799068014889599 1102 96-97 -0.6949340233805508 1102 98-99 -0.901919271506241 1102 100-101 -0.4757825646294336 1103 1 0.20632694815662944 1103 2 0.29519981742824086 1103 3 0.3221505104265603 1103 4 0.25792285790281255 1103 5 0.31367176457225554 1103 6 0.13940252047778756 1103 7 0.21013469072972413 1103 8 -0.034222609906130685 1103 9 0.40021205882469957 1103 10-11 0.3797260066858925 1103 12-13 0.34869503848442207 1103 14-15 0.4027394530873494 1103 16-17 0.4409430571529924 1103 18-19 0.2850581771777314 1103 20-21 0.28951395845737693 1103 22-23 0.21742344693003446 1103 24-25 0.44014867458990636 1103 26-27 0.30421918259652614 1103 28-29 0.3636444743242393 1103 30-31 0.18374262368873673 1103 32-33 0.21586950851962428 1103 34-35 0.2690786086751302 1103 36-37 0.4568198499855356 1103 38-39 0.3515854934572076 1103 40-41 0.27640900440269434 1103 42-43 0.40058671144986846 1103 44-45 0.5389185737002151 1103 46-47 0.6396761682129366 1103 48-49 0.5266781559011875 1103 50-51 0.05251073788271299 1103 52-53 0.29163047452132673 1103 54-55 0.5568845727383192 1103 56-57 0.6091325679431563 1103 58-59 0.5711591005676269 1103 60-61 0.6317998585607398 1103 62-63 0.6075692743784273 1103 64-65 0.5012828903733535 1103 66-67 0.608036617578783 1103 68-69 0.44143943428996835 1103 70-71 0.7356561426029167 1103 72-73 0.47935623952249884 1103 74-75 0.3495278646050828 1103 76-77 0.40561855912750744 1103 78-79 0.49581253768149836 1103 80-81 0.6961336424712492 1103 82-83 0.6108875632469868 1103 84-85 0.47120705876061564 1103 86-87 0.4706030754117805 1103 88-89 0.46983436007791113 1103 90-91 0.4627189133843501 1103 92-93 0.46672658290478 1103 94-95 0.504048212618315 1103 96-97 0.659758888951874 1103 98-99 0.45316681787199187 1103 100-101 0.33980589472106715 1104 1 0.273711159937708 1104 2 0.2163968169460695 1104 3 0.3088979826821934 1104 4 0.33586487250251906 1104 5 0.2753517986216636 1104 6 0.20531084352008122 1104 7 0.2430803203975671 1104 8 0.25740320543433626 1104 9 0.4067612313080531 1104 10-11 0.43479223336894535 1104 12-13 0.4069540675010188 1104 14-15 0.4574548377739873 1104 16-17 0.22724292400732793 1104 18-19 0.37011575743687075 1104 20-21 0.32538619663552026 1104 22-23 0.4323902555829946 1104 24-25 0.40541501223580667 1104 26-27 0.39882945241272694 1104 28-29 0.31839012271340295 1104 30-31 0.34124508451899516 1104 32-33 0.2720290922041855 1104 34-35 0.21770210383903077 1104 36-37 0.3906911613606354 1104 38-39 0.4993130708935283 1104 40-41 0.1768570851501039 1104 42-43 0.27784442266077747 1104 44-45 0.1488838636832881 1104 46-47 0.23961933388154222 1104 48-49 0.13218201790552797 1104 50-51 -0.050193091749335395 1104 52-53 0.17099147039824913 1104 54-55 0.541579159204268 1104 56-57 0.45536200069192745 1104 58-59 0.42965477881827496 1104 60-61 0.395225217276419 1104 62-63 0.28640898620654553 1104 64-65 0.3183792130470735 1104 66-67 0.36024197977545214 1104 68-69 0.3272794586276859 1104 70-71 0.26662981720387435 1104 72-73 -0.01081939233437268 1104 74-75 -0.0740244749022203 1104 76-77 0.02302252824317108 1104 78-79 0.305226779636083 1104 80-81 0.20239285382573158 1104 82-83 0.005204593264974022 1104 84-85 0.10099940076410263 1104 86-87 0.06003006151753709 1104 88-89 -0.1271224131654165 1104 90-91 -0.0519367995202451 1104 92-93 -0.23850513735573742 1104 94-95 -0.09347891501870009 1104 96-97 -0.06100269352652532 1104 98-99 0.1478066079006446 1104 100-101 0.22263874547652662 1105 1 0.25828943515711345 1105 2 0.3961213263789993 1105 3 0.282894943260775 1105 4 0.43211911949699555 1105 5 0.4060858735306496 1105 6 0.4329360881206554 1105 7 0.46889749373564626 1105 8 0.31546738538750674 1105 9 0.5913332334206771 1105 10-11 0.5683704539516228 1105 12-13 0.48145935208758317 1105 14-15 0.6373548930178004 1105 16-17 0.49062260076020436 1105 18-19 0.4358513399150823 1105 20-21 0.1969306759980114 1105 22-23 8.865109429549989E-4 1105 24-25 0.035186367611359515 1105 26-27 0.06448904935550104 1105 28-29 0.2813944754400808 1105 30-31 0.20784392743433244 1105 32-33 0.13507022354887965 1105 34-35 0.029591108296983748 1105 36-37 0.018901069631624523 1105 38-39 -0.007652607118018295 1105 40-41 -0.04844230393022997 1105 42-43 0.040237855729763794 1105 44-45 0.2167512906774789 1105 46-47 0.02367825656650524 1105 48-49 0.15676222564655262 1105 50-51 0.14369320920194895 1105 52-53 0.21762670745086155 1105 54-55 0.2588740134354808 1105 56-57 0.29826519451271594 1105 58-59 0.3040704692748619 1105 60-61 0.3109341390507794 1105 62-63 0.23739502753215191 1105 64-65 0.33972548247094636 1105 66-67 0.02188552363559637 1105 68-69 0.22647645036943942 1105 70-71 0.23451302814417474 1105 72-73 0.10827655179855711 1105 74-75 0.2409836295470278 1105 76-77 0.24010692985961057 1105 78-79 0.20592274036238578 1105 80-81 0.3081511105495913 1105 82-83 0.3586582420883637 1105 84-85 0.0879892808263456 1105 86-87 0.12275935874745159 1105 88-89 0.16996028864144108 1105 90-91 0.12364872417287032 1105 92-93 0.26031941396535885 1105 94-95 0.23557538377949427 1105 96-97 0.3997008455174935 1105 98-99 0.3124405012942546 1105 100-101 0.4010833387667283 1106 1 0.016775261183223478 1106 2 0.04672765208099605 1106 3 -0.005389322710804834 1106 4 0.10695915266011014 1106 5 0.24815217032794834 1106 6 0.14760695678093327 1106 7 0.33242834074631133 1106 8 0.42541708343384244 1106 9 0.49607035997414783 1106 10-11 0.46033211711253585 1106 12-13 0.4947160566051423 1106 14-15 0.33242479685632276 1106 16-17 0.253953499082229 1106 18-19 0.3768411759553132 1106 20-21 0.38286041571378604 1106 22-23 0.3981555612044261 1106 24-25 0.24588520621873755 1106 26-27 0.11153639880319588 1106 28-29 0.1868141981379452 1106 30-31 0.1912611192625846 1106 32-33 0.16913332863254027 1106 34-35 0.07865529375810354 1106 36-37 0.15823854590144038 1106 38-39 0.150963757945469 1106 40-41 0.20647788219961427 1106 42-43 0.25279830823705396 1106 44-45 0.02457809959997803 1106 46-47 0.12020991635748857 1106 48-49 -0.02075732760817317 1106 50-51 0.19992054690480643 1106 52-53 0.07734863248896318 1106 54-55 0.3215010760758119 1106 56-57 0.27113326281359207 1106 58-59 0.28768191875236226 1106 60-61 0.36405631160309326 1106 62-63 0.3986055470964516 1106 64-65 0.14513911983107874 1106 66-67 0.12554333734067313 1106 68-69 0.03123351527568019 1106 70-71 0.24191111648067576 1106 72-73 0.3393258506572465 1106 74-75 0.06723573707166253 1106 76-77 -0.020837017739719244 1106 78-79 0.10676058490684781 1106 80-81 0.03365644330321871 1106 82-83 0.160770419440599 1106 84-85 0.027528296121442253 1106 86-87 0.1304286586260588 1106 88-89 0.18678624745671613 1106 90-91 0.3342583199224407 1106 92-93 0.2908695179812604 1106 94-95 0.3025846781799828 1106 96-97 0.37441501865021465 1106 98-99 0.47727271683560346 1106 100-101 0.8226515500139051 1107 1 0.5370079633626972 1107 2 0.5300530946399427 1107 3 0.5482972362738145 1107 4 0.47581269880654986 1107 5 0.49828950223013635 1107 6 0.5214303936779743 1107 7 0.7607525996890985 1107 8 0.8346577207057493 1107 9 0.7775757900159448 1107 10-11 0.8847891129325873 1107 12-13 0.8592322980887843 1107 14-15 0.9783558325691004 1107 16-17 1.0436723334959481 1107 18-19 1.112925335928331 1107 20-21 1.1392765070565218 1107 22-23 1.1656250871418408 1107 24-25 1.2636585810164718 1107 26-27 1.060469928216861 1107 28-29 1.0081129655557461 1107 30-31 0.858913619286902 1107 32-33 1.0323848843581587 1107 34-35 0.980559861867917 1107 36-37 1.0532534140660488 1107 38-39 1.0930990339225914 1107 40-41 0.9860976333483649 1107 42-43 1.1345411911368828 1107 44-45 1.0376328183371086 1107 46-47 1.228767704389675 1107 48-49 1.3334454825884379 1107 50-51 1.2700104855189096 1107 52-53 1.2686589442177905 1107 54-55 1.5151705942305398 1107 56-57 1.4649501655008876 1107 58-59 1.5241022743560535 1107 60-61 1.5016224238617895 1107 62-63 1.526758100626708 1107 64-65 1.3303972616509938 1107 66-67 1.5046872910077767 1107 68-69 1.6348168726596626 1107 70-71 1.5953911911944196 1107 72-73 1.4564256992192348 1107 74-75 1.2562459421128942 1107 76-77 1.5831002895173079 1107 78-79 1.6721223823171627 1107 80-81 1.716219507344963 1107 82-83 1.760136130891194 1107 84-85 1.834731005791749 1107 86-87 1.7377607553777636 1107 88-89 1.7260629282727287 1107 90-91 1.6177044751155982 1107 92-93 1.8089170701657622 1107 94-95 1.896716049845189 1107 96-97 1.9902351835782106 1107 98-99 2.177670120014181 1107 100-101 2.482170742430977 1108 1 0.26036543185291094 1108 2 0.11820354079135953 1108 3 0.0775433891591959 1108 4 0.25077917775532654 1108 5 0.3890986728550416 1108 6 0.27961026488366514 1108 7 0.3939116235560931 1108 8 0.19433253895736868 1108 9 0.36288530411682984 1108 10-11 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-3.474272033639057 2316 68-69 -3.1022412053409596 2316 70-71 -2.5972960651462813 2316 72-73 -2.3843389648088724 2316 74-75 -2.4733415232374547 2316 76-77 -3.2705318090285154 2316 78-79 -3.1607936728851023 2316 80-81 -3.267817565927004 2316 82-83 -3.123912430499832 2316 84-85 -3.219229801193027 2316 86-87 -3.1576695673272077 2316 88-89 -3.608492592694944 2316 90-91 -3.7474409422366506 2316 92-93 -3.994194727761929 2316 94-95 -3.831154126997731 2316 96-97 -4.278637943117214 2316 98-99 -4.721263708420029 2316 100-101 -5.075644513791133 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6883.0 3 2773.0 4 2865.0 5 3077.0 6 3426.0 7 3949.0 8 4844.0 9 6144.0 10 7634.0 11 9163.0 12 10644.0 13 12332.0 14 13688.0 15 15788.0 16 18071.0 17 21244.0 18 19411.0 19 16005.0 20 17015.0 21 18509.0 22 20614.0 23 23558.0 24 27051.0 25 31514.0 26 36748.0 27 43167.0 28 51404.0 29 61255.0 30 73881.0 31 89989.0 32 110955.0 33 140467.0 34 182886.0 35 244901.0 36 337423.0 37 433830.0 38 330940.0 39 48326.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.284106477980686 18.05756499802564 14.241493863495752 23.416834660497923 2 15.54767818498988 19.243878996661 40.22907429128642 24.979368527062697 3 19.703874576719606 23.17967950385723 29.180439640106258 27.9360062793169 4 13.014285937004683 15.995156604842355 36.76023395085689 34.23032350729607 5 14.493326094239883 36.462522099091885 33.91993711903804 15.124214687630186 6 32.974347593999845 35.05756846939245 17.340474662476236 14.627609274131476 7 29.626242341293697 31.658917356323308 20.19052430959721 18.524315992785787 8 26.655914365314437 34.463124402927505 19.366853333013562 19.5141078987445 9 26.185546820356432 17.01995595541185 20.2528367192777 36.54166050495402 10-11 25.498270782246895 25.897986847110605 27.086060691835023 21.517681678807474 12-13 26.22905499746636 23.132038095542693 27.567876050560848 23.0710308564301 14-15 23.05113212030518 24.946296255216744 25.712627559508782 26.28994406496929 16-17 22.793627267878065 28.106868791883333 26.363374917135175 22.73612902310342 18-19 22.59633603287417 26.799405194950555 28.3743993987992 22.22985937337608 20-21 23.248122819215663 25.917495778579102 28.40356244155513 22.4308189606501 22-23 22.914640828664165 25.73366809869183 28.4061156227431 22.9455754499009 24-25 22.987327527106416 26.01017018850755 28.333640049498193 22.668862234887836 26-27 22.786426097577984 26.147032160000546 28.447380657083148 22.619161085338327 28-29 22.48879354751308 26.409563319004153 28.504991102374444 22.59665203110832 30-31 22.70739583250074 26.00872425286346 28.361015418308543 22.92286449632726 32-33 22.53781642230989 26.161607159983884 28.414907255254256 22.885669162451965 34-35 23.195246054446983 26.19385635659387 28.034771419180228 22.576126169778917 36-37 23.00751305778195 25.92847178941618 28.215739195645828 22.848275957156048 38-39 22.7436844024598 26.264932885067864 28.271776759799977 22.71960595267236 40-41 22.948109046273714 26.126051059916783 28.19393233133337 22.731907562476135 42-43 22.77731443868945 26.188524704809485 27.822437715970523 23.211723140530545 44-45 22.909279458749857 26.219741003974256 27.853781737603178 23.017197799672708 46-47 23.07733192664437 26.4536052399164 27.50100905955649 22.96805377388274 48-49 23.15450322193916 26.43482691443129 27.451600979069884 22.95906888455967 50-51 22.8154215621414 26.623336790745196 27.53417932358478 23.02706232352863 52-53 23.176073717330713 26.71052376557499 27.131644627222318 22.98175788987198 54-55 22.857962664019535 26.425215843704166 27.343059862372353 23.373761629903953 56-57 22.94529861143997 26.81155901844693 27.195579498473528 23.04756287163957 58-59 23.201051979344854 26.466947511529753 27.26766108633532 23.064339422790077 60-61 23.156211068118495 26.473089983785353 27.68136574663793 22.68933320145822 62-63 22.912605917492705 26.64556126105731 27.519935676734026 22.92189714471596 64-65 22.728346282050925 26.705096536915736 27.34840289380959 23.21815428722375 66-67 22.82812309865048 26.80552899702258 27.061529943338908 23.304817960988032 68-69 22.69493441673637 26.610667486199358 27.47499339591462 23.219404701149653 70-71 22.92770344490358 26.533688788281896 27.27974851982029 23.25885924699423 72-73 22.896410253950975 26.478083710387352 27.434426170545905 23.191079865115768 74-75 22.65205778572509 26.975337023118804 27.110551895276114 23.26205329587999 76-77 22.87036321032102 26.694928547036827 27.11547987350091 23.31922836914124 78-79 22.83947372365995 26.709513649105798 27.165872988112312 23.28513963912194 80-81 22.926131344615136 26.941686258866454 27.03725569039821 23.0949267061202 82-83 22.942927221592242 26.807631017618878 27.18412652423033 23.065315236558547 84-85 22.899844776348456 26.811568718038075 26.965173791341684 23.323412714271782 86-87 22.87079987802826 26.775254284297255 27.12202648928811 23.231919348386374 88-89 22.81452304859173 26.761639591776543 26.90311187060322 23.520725489028504 90-91 22.84883783668205 26.695425164691596 27.10923739689165 23.346499601734703 92-93 23.04529520357882 26.47975482533743 27.24522622022795 23.229723750855797 94-95 22.75142667996949 26.479308119911654 27.31150729620492 23.457757903913937 96-97 22.686153276216537 26.478185365101183 27.411107263538998 23.42455409514329 98-99 22.562238704761036 26.591034316949592 27.093124878714242 23.753602099575133 100-101 22.450499166057668 26.474091018848856 27.29516041596372 23.780249399129758 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 157.0 1 118.0 2 117.5 3 164.0 4 164.0 5 186.0 6 293.5 7 594.5 8 1004.5 9 1274.0 10 1276.0 11 1239.5 12 1355.0 13 1519.5 14 1683.5 15 1864.0 16 2031.5 17 2204.5 18 2390.5 19 2662.0 20 2976.5 21 3362.5 22 3998.5 23 4989.0 24 6236.5 25 7857.5 26 10063.5 27 12811.0 28 15733.0 29 19469.5 30 24047.5 31 29097.5 32 34698.0 33 39557.0 34 44803.0 35 50146.0 36 56068.5 37 61752.5 38 65152.5 39 67534.0 40 69592.5 41 73565.5 42 78394.5 43 82656.0 44 86424.0 45 89999.0 46 92230.0 47 95786.5 48 104692.5 49 105532.0 50 98266.5 51 94035.0 52 91365.5 53 90630.0 54 89559.0 55 84294.0 56 77218.0 57 70141.5 58 63532.0 59 55783.5 60 46520.5 61 38400.5 62 31294.5 63 24845.5 64 19605.5 65 15391.0 66 11800.0 67 9720.5 68 7600.5 69 5268.5 70 3892.0 71 2968.0 72 2309.0 73 1830.5 74 1430.0 75 1131.5 76 778.5 77 466.5 78 282.5 79 181.0 80 113.5 81 72.0 82 47.0 83 41.0 84 32.0 85 22.5 86 20.0 87 10.0 88 6.5 89 8.0 90 6.0 91 3.5 92 3.5 93 4.5 94 3.0 95 0.5 96 1.0 97 2.0 98 1.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012428133618021513 2 0.0056745819091931025 3 0.008152216263911218 4 0.033408101944263614 5 0.04607600662887076 6 0.058024598113721015 7 0.02633485547998771 8 0.023857221125269595 9 0.015065615350463376 10-11 0.04441759008498686 12-13 0.04174014650811405 14-15 0.01864220042703227 16-17 0.02283819409228069 18-19 0.029052260901291448 20-21 0.02251849933683319 22-23 0.013427179728794945 24-25 0.0029371955656738945 26-27 0.0028173100323810826 28-29 0.0013587027106518696 30-31 0.006673628019966535 32-33 0.001398664555082807 34-35 0.0017783020771767117 36-37 0.005734524675839508 38-39 0.005974295742425132 40-41 0.0036565087654307667 42-43 7.992368886187468E-4 44-45 0.001278779021789995 46-47 0.002277825132563429 48-49 4.7954213317124813E-4 50-51 0.001338721788436401 52-53 6.993322775414035E-4 54-55 0.0022178823659170224 56-57 0.0018582257660385865 58-59 0.0022578442103479597 60-61 7.792559664032782E-4 62-63 0.0010589888774198395 64-65 0.003196947554474987 66-67 0.0020979968326242104 68-69 0.005914352975778726 70-71 0.009650785430071368 72-73 0.003716451532077173 74-75 0.0029371955656738945 76-77 0.0030570810989667065 78-79 0.03394758684408127 80-81 0.0216193578371371 82-83 0.003976203520878265 84-85 0.007632712286309033 86-87 0.006713589864397473 88-89 0.005195039776021855 90-91 0.01142908750724808 92-93 0.01334725603993307 94-95 0.006453837875596381 96-97 0.0034966613877070174 98-99 0.021879109825938194 100-101 0.0405612720974014 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2502387.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.06568392249495 #Duplication Level Percentage of deduplicated Percentage of total 1 80.05652800455917 48.08650107054216 2 11.721151908982975 14.080780115450395 3 3.567319584169576 6.428204717797676 4 1.5410222480874207 3.702502210846065 5 0.7978674617216094 2.3962227383906765 6 0.5105915711922339 1.840141915723368 7 0.3386040982611182 1.4236940718709625 8 0.22991954606809664 1.1048219825383847 9 0.18151197335918257 0.9812376737946887 >10 0.9334612214137928 10.345000465926404 >50 0.0789720133436955 3.268596491147264 >100 0.040448129992965036 4.405393874423035 >500 0.0017348258988029153 0.706259288158876 >1k 8.006888763654863E-4 0.9965862818791315 >5k 6.672407303045719E-5 0.2340571015110644 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5840 0.23337717147667408 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4745 0.18961895182479768 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3985 0.15924795005728531 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2753 0.1100149577183705 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2737 0.1093755682074755 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 3.9961844430937344E-5 3 0.0 0.0 0.0 0.0 3.9961844430937344E-5 4 0.0 0.0 0.0 0.0 3.9961844430937344E-5 5 0.0 0.0 0.0 0.0 3.9961844430937344E-5 6 0.0 0.0 0.0 0.0 3.9961844430937344E-5 7 0.0 0.0 0.0 0.0 3.9961844430937344E-5 8 0.0 0.0 0.0 0.0 3.9961844430937344E-5 9 0.0 0.0 0.0 0.0 3.9961844430937344E-5 10-11 0.0 0.0 0.0 0.0 3.9961844430937344E-5 12-13 0.0 0.0 0.0 0.0 3.9961844430937344E-5 14-15 0.0 0.0 0.0 0.0 3.9961844430937344E-5 16-17 0.0 0.0 0.0 0.0 3.9961844430937344E-5 18-19 0.0 0.0 0.0 0.0 3.9961844430937344E-5 20-21 0.0 0.0 0.0 1.9980922215468672E-5 3.9961844430937344E-5 22-23 0.0 0.0 0.0 1.1988553329281203E-4 3.9961844430937344E-5 24-25 0.0 0.0 0.0 2.3977106658562404E-4 5.9942766646406016E-5 26-27 0.0 0.0 0.0 5.394848998176541E-4 1.1988553329281202E-4 28-29 0.0 0.0 0.0 0.0011988553329281202 1.1988553329281202E-4 30-31 0.0 0.0 0.0 0.002417691588071709 1.1988553329281202E-4 32-33 3.9961844430937344E-5 0.0 0.0 0.004036146287524672 1.1988553329281202E-4 34-35 3.9961844430937344E-5 0.0 0.0 0.005634620064762165 1.1988553329281202E-4 36-37 3.9961844430937344E-5 0.0 0.0 0.007193131997568721 1.1988553329281202E-4 38-39 3.9961844430937344E-5 0.0 0.0 0.01023023217431996 1.1988553329281202E-4 40-41 3.9961844430937344E-5 0.0 0.0 0.014206435695198225 1.1988553329281202E-4 42-43 3.9961844430937344E-5 0.0 0.0 0.017862944460628992 1.1988553329281202E-4 44-45 3.9961844430937344E-5 3.9961844430937344E-5 0.0 0.020000903137684142 1.1988553329281202E-4 46-47 3.9961844430937344E-5 3.9961844430937344E-5 0.0 0.023477583603175686 1.1988553329281202E-4 48-49 3.9961844430937344E-5 3.9961844430937344E-5 0.0 0.028053214790518012 1.1988553329281202E-4 50-51 3.9961844430937344E-5 3.9961844430937344E-5 0.0 0.03274873151115315 1.1988553329281202E-4 52-53 3.9961844430937344E-5 3.9961844430937344E-5 0.0 0.0415203563637439 1.1988553329281202E-4 54-55 3.9961844430937344E-5 1.1988553329281202E-4 0.0 0.04739474749509169 1.1988553329281202E-4 56-57 3.9961844430937344E-5 1.1988553329281202E-4 0.0 0.06489803535584224 1.1988553329281202E-4 58-59 3.9961844430937344E-5 1.1988553329281202E-4 0.0 0.09446980023473588 1.1988553329281202E-4 60-61 3.9961844430937344E-5 1.1988553329281202E-4 0.0 0.11628896729402766 1.1988553329281202E-4 62-63 3.9961844430937344E-5 1.1988553329281202E-4 0.0 0.12973612794503808 1.1988553329281202E-4 64-65 3.9961844430937344E-5 1.1988553329281202E-4 0.0 0.15750960982453954 1.1988553329281202E-4 66-67 3.9961844430937344E-5 1.1988553329281202E-4 0.0 0.2132164209612662 1.1988553329281202E-4 68-69 3.9961844430937344E-5 1.1988553329281202E-4 0.0 0.28700596670299194 1.1988553329281202E-4 70-71 3.9961844430937344E-5 1.1988553329281202E-4 0.0 0.35150438361452485 1.5984737772374938E-4 72-73 3.9961844430937344E-5 1.1988553329281202E-4 0.0 0.42237671471279226 2.3977106658562404E-4 74-75 3.9961844430937344E-5 1.1988553329281202E-4 0.0 0.512111036382462 2.3977106658562404E-4 76-77 3.9961844430937344E-5 1.1988553329281202E-4 0.0 0.5703754055627687 2.3977106658562404E-4 78-79 3.9961844430937344E-5 1.1988553329281202E-4 0.0 0.6485607541918976 2.3977106658562404E-4 80-81 3.9961844430937344E-5 1.1988553329281202E-4 0.0 0.7553587834335775 2.3977106658562404E-4 82-83 3.9961844430937344E-5 1.1988553329281202E-4 0.0 0.851726771278783 2.797329110165614E-4 84-85 3.9961844430937344E-5 1.1988553329281202E-4 0.0 0.9851993316781138 2.797329110165614E-4 86-87 3.9961844430937344E-5 1.1988553329281202E-4 0.0 1.1928810371856953 2.797329110165614E-4 88-89 3.9961844430937344E-5 1.1988553329281202E-4 0.0 1.4614845745282405 2.797329110165614E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8625 0.0 27.549093 1 TACATGG 9025 0.0 25.221294 2 ACATGGG 8935 0.0 24.677574 3 GGTATCA 9575 0.0 23.972027 1 GTATCAA 12695 0.0 23.920187 1 GTATAGG 740 0.0 22.47671 1 GAGTACT 6755 0.0 21.452374 12-13 TCAACGC 14535 0.0 20.564747 4 ATCAACG 14615 0.0 20.516798 3 TATCAAC 14790 0.0 20.498947 2 CAACGCA 14755 0.0 20.193302 5 CATGGGG 5530 0.0 20.108444 4 GTACTTT 7200 0.0 19.959932 14-15 AACGCAG 14950 0.0 19.86475 6 CATGGGA 3645 0.0 18.513075 4 GTACCTG 2050 0.0 18.313429 1 AGAGTAC 11365 0.0 18.228392 10-11 ACGCAGA 16380 0.0 18.101517 7 AGTACTT 7310 0.0 18.068758 12-13 CGCAGAG 16600 0.0 17.718498 8 >>END_MODULE