FastQCFastQC Report
Thu 26 May 2016
SRR936626_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936626_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502387
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68150.272339969796838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61010.24380721287314872No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52100.20820120948518353No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36240.14482172421771694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG82350.024.7750341
GTATCAA146850.023.0791441
TACATGG88200.022.2984282
ACATGGG86700.021.585773
GAGTACT86200.021.01713812-13
GTGTAGC12700.020.7896841
GGTATCA112700.020.4884971
GTACTTT89550.020.230914-15
AGTACTT91150.019.87577812-13
AGAGTAC131550.019.85485610-11
TCAACGC171750.019.4408054
ATCAACG172150.019.3971863
TATCAAC173950.019.3080082
CAACGCA173850.019.2317565
AACGCAG177000.019.0236556
GGACCGT3557.8216544E-1118.7292616
CATGGGG54150.017.8931754
TACCTGG19150.017.861022
GTACCTG20900.017.6861521
ACTTTTT106000.017.58444816-17