##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936626_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2502387 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.529508824973917 31.0 31.0 34.0 30.0 34.0 2 31.766733123213957 31.0 31.0 34.0 30.0 34.0 3 32.0462034849126 33.0 31.0 34.0 30.0 34.0 4 35.83377031610218 37.0 35.0 37.0 35.0 37.0 5 35.64772994744618 37.0 35.0 37.0 33.0 37.0 6 35.74349571029581 37.0 35.0 37.0 33.0 37.0 7 35.67819805649566 37.0 35.0 37.0 33.0 37.0 8 35.65494465883974 37.0 35.0 37.0 33.0 37.0 9 37.30502915815979 39.0 37.0 39.0 34.0 39.0 10-11 37.19452207032725 39.0 37.0 39.0 33.0 39.0 12-13 37.06840009159255 39.0 37.0 39.0 33.0 39.0 14-15 38.23091132586606 40.0 38.0 41.0 33.0 41.0 16-17 38.14103753735933 40.0 37.0 41.0 33.0 41.0 18-19 38.177638790482845 40.0 37.5 41.0 33.5 41.0 20-21 38.24428355805877 40.0 38.0 41.0 33.0 41.0 22-23 38.16059566326072 40.0 37.5 41.0 33.0 41.0 24-25 38.06129547508039 40.0 37.0 41.0 33.0 41.0 26-27 37.87236986125647 40.0 37.0 41.0 33.0 41.0 28-29 37.7992806468384 40.0 37.0 41.0 32.5 41.0 30-31 37.81068755552199 40.0 37.0 41.0 33.0 41.0 32-33 37.669101541847844 40.0 37.0 41.0 33.0 41.0 34-35 37.52258143924182 40.0 36.5 41.0 32.0 41.0 36-37 37.3107165278592 39.5 36.0 41.0 31.5 41.0 38-39 37.175053458957386 39.0 36.0 41.0 31.0 41.0 40-41 36.96444674624668 39.0 36.0 41.0 31.0 41.0 42-43 36.75257524115974 39.0 35.5 41.0 30.5 41.0 44-45 36.51143128540869 39.0 35.0 40.0 30.0 41.0 46-47 36.176886508761434 39.0 35.0 40.0 30.0 41.0 48-49 35.773243906717866 38.5 35.0 40.0 28.5 41.0 50-51 35.592003954624126 38.0 35.0 40.0 28.0 41.0 52-53 35.31633935917985 38.0 34.0 40.0 27.5 41.0 54-55 35.04677893547241 38.0 34.0 40.0 27.0 41.0 56-57 34.76654210559758 38.0 34.0 40.0 26.0 41.0 58-59 34.39693420721895 37.5 33.5 40.0 26.0 41.0 60-61 34.02844963628728 37.0 33.0 40.0 25.0 41.0 62-63 33.72534803769361 36.5 33.0 39.0 25.0 40.5 64-65 33.29199420393408 36.0 32.0 39.0 23.5 40.0 66-67 32.88162682270968 35.5 32.0 39.0 23.0 40.0 68-69 32.422851261615406 35.0 31.0 38.0 22.0 40.0 70-71 31.956737107409843 35.0 31.0 37.5 21.0 39.5 72-73 31.539502682838425 35.0 31.0 37.0 20.0 39.0 74-75 31.158276078000725 34.0 30.0 36.5 19.5 39.0 76-77 29.731597670544165 32.5 28.5 35.0 18.0 37.0 78-79 30.536268370959405 34.0 30.0 35.0 18.0 37.5 80-81 30.385747488298172 34.0 30.0 35.0 18.0 37.0 82-83 30.0518005408436 34.0 30.0 35.0 15.0 37.0 84-85 29.60569987775672 34.0 29.0 35.0 9.5 36.0 86-87 29.226423610736468 34.0 29.0 35.0 7.0 36.0 88-89 28.82393930275373 34.0 29.0 35.0 2.0 35.0 90-91 28.367122871082692 33.0 28.5 35.0 2.0 35.0 92-93 27.90516874488239 33.0 27.0 35.0 2.0 35.0 94-95 27.369294997136734 33.0 26.5 34.5 2.0 35.0 96-97 26.68523633634606 32.5 25.0 34.0 2.0 35.0 98-99 25.81161247241134 32.0 24.5 34.0 2.0 35.0 100-101 22.97512535031552 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2980867072650355 1101 2 0.3178607494445167 1101 3 0.13263469233802994 1101 4 0.04908725961115579 1101 5 0.12818198007725812 1101 6 0.14648806974337703 1101 7 0.13156801601660817 1101 8 0.11631506363851685 1101 9 0.1453959343615523 1101 10-11 0.17237161940721535 1101 12-13 0.22166701493526375 1101 14-15 0.25588379418866225 1101 16-17 0.21729149110760915 1101 18-19 0.21725634378698544 1101 20-21 0.04923155807571078 1101 22-23 -0.038380434431289245 1101 24-25 -0.027577267346401868 1101 26-27 0.03487785404899313 1101 28-29 0.07002389387412933 1101 30-31 -0.06599072026707375 1101 32-33 0.027926088160867835 1101 34-35 1.0728744849330951E-4 1101 36-37 -0.029317166622810475 1101 38-39 0.01648839648760969 1101 40-41 -0.008401408550547274 1101 42-43 -0.022167732005272 1101 44-45 -0.0012837451473544093 1101 46-47 0.005179777613093961 1101 48-49 -0.020296138329520375 1101 50-51 -0.09980602530617944 1101 52-53 0.04807413937161442 1101 54-55 -0.04669571858227073 1101 56-57 -0.09446683273984036 1101 58-59 -0.05609132826467089 1101 60-61 -0.03521131071135386 1101 62-63 -0.08988119651615989 1101 64-65 -0.04116931589334172 1101 66-67 -0.301085840566067 1101 68-69 -0.48866563706713606 1101 70-71 -0.4787292005496191 1101 72-73 -0.3015182466191213 1101 74-75 -0.26264990192059656 1101 76-77 0.04675613534390166 1101 78-79 -0.044573319638608666 1101 80-81 -0.09175172179921987 1101 82-83 -0.038490856853567124 1101 84-85 -0.09532562202224426 1101 86-87 -0.1255122844836407 1101 88-89 -0.19797776211290596 1101 90-91 -0.4396357292512434 1101 92-93 -0.23140305379781978 1101 94-95 -0.25959785524684165 1101 96-97 -0.41159675614276026 1101 98-99 -0.3778371557591633 1101 100-101 -0.39538367315061507 1102 1 0.38674101894407187 1102 2 0.3793177059171491 1102 3 0.15060396462295245 1102 4 0.10290655036445173 1102 5 0.1956312378316838 1102 6 0.10348727911370759 1102 7 0.08198118870051019 1102 8 -0.13289201722854926 1102 9 -0.004355124135884125 1102 10-11 0.04899065496113053 1102 12-13 0.05622317864139603 1102 14-15 0.12772852551194802 1102 16-17 0.21028953437549802 1102 18-19 0.17399608010130407 1102 20-21 0.17145939373463648 1102 22-23 -2.0355317531084438E-4 1102 24-25 0.08903450296193682 1102 26-27 -0.13340110695984464 1102 28-29 0.08025705330963717 1102 30-31 0.1364036055150848 1102 32-33 0.19036919487756165 1102 34-35 0.26225520540617 1102 36-37 0.2575539546365775 1102 38-39 0.06486409515697034 1102 40-41 0.25031383350728476 1102 42-43 0.13931608756022484 1102 44-45 0.09538217871409671 1102 46-47 0.17006422052443781 1102 48-49 0.16072294972953216 1102 50-51 0.11308322243564106 1102 52-53 -0.07687205812706566 1102 54-55 0.17074880760466016 1102 56-57 0.24194380016073325 1102 58-59 0.24101982083465145 1102 60-61 0.34955975773763726 1102 62-63 0.15382581871948986 1102 64-65 0.07066967649646472 1102 66-67 0.05255685587793124 1102 68-69 -0.03191674288025581 1102 70-71 -0.20983956207190246 1102 72-73 -0.07608805555537046 1102 74-75 -0.4841101552273557 1102 76-77 -0.1671809256505803 1102 78-79 0.04988174115440103 1102 80-81 -0.09951836089051369 1102 82-83 0.08381405756611215 1102 84-85 0.34359213112425024 1102 86-87 0.0837115247639808 1102 88-89 -0.07469066586061501 1102 90-91 0.03668659123156459 1102 92-93 0.13901075993716105 1102 94-95 -0.15111217052797699 1102 96-97 -0.1701799722044477 1102 98-99 -0.5953595238629035 1102 100-101 -0.5495907998070457 1103 1 0.41000493809709226 1103 2 0.423804958336337 1103 3 0.22182594457312632 1103 4 0.09756852362793467 1103 5 0.0217455146953327 1103 6 0.06305353597404206 1103 7 -0.003221081961022776 1103 8 0.1267176292078105 1103 9 -0.02312555726973642 1103 10-11 0.10218306775900032 1103 12-13 0.16223110275363695 1103 14-15 0.14503713890915293 1103 16-17 0.04229401474567851 1103 18-19 0.14201710407384383 1103 20-21 0.04888795504527366 1103 22-23 0.013751280887277062 1103 24-25 0.010322246915038136 1103 26-27 0.09398310493391193 1103 28-29 -0.008175767428937775 1103 30-31 0.0792170027948842 1103 32-33 -0.055209903341818745 1103 34-35 0.01253788151877444 1103 36-37 -0.0200616504441129 1103 38-39 -0.009140648245377747 1103 40-41 0.041619392345111805 1103 42-43 0.03312817838335036 1103 44-45 0.13843062373018 1103 46-47 0.036608425011316115 1103 48-49 0.06996457171987913 1103 50-51 -0.14233603306886522 1103 52-53 -0.03442700126573328 1103 54-55 -0.03556606288180575 1103 56-57 -0.09130757905902698 1103 58-59 -0.022658418094657407 1103 60-61 -0.10095967117017324 1103 62-63 -0.19990540461735407 1103 64-65 -0.18304177769581287 1103 66-67 -0.1448292888133622 1103 68-69 -0.3081975133808754 1103 70-71 -0.38735038353232554 1103 72-73 -0.23520880317720483 1103 74-75 -0.17872581766003037 1103 76-77 -0.2989736925522166 1103 78-79 -0.21614453082252538 1103 80-81 -0.1314425484865822 1103 82-83 -0.05525734350464262 1103 84-85 -0.059952745966640464 1103 86-87 -0.11669531660008303 1103 88-89 -0.04812960394515642 1103 90-91 -0.10323018520075777 1103 92-93 -0.06284114866166846 1103 94-95 -0.03942449248792812 1103 96-97 -0.24024126012963976 1103 98-99 -0.17775101087339706 1103 100-101 0.04539654162098472 1104 1 0.2790810895194511 1104 2 0.30892525990566 1104 3 0.10264009789394635 1104 4 -9.89983190478938E-4 1104 5 -0.03781011225693476 1104 6 0.04663410616510788 1104 7 -0.03496055133825138 1104 8 -0.048959568366022665 1104 9 -0.08234527296887961 1104 10-11 -0.15092142989279012 1104 12-13 -0.05056465394131493 1104 14-15 0.037733848100195644 1104 16-17 -0.026704789332249845 1104 18-19 0.09142930813432315 1104 20-21 -0.004595369081783929 1104 22-23 0.00654856844047913 1104 24-25 0.02089175056106285 1104 26-27 -0.007912209713531126 1104 28-29 -0.04625196614213678 1104 30-31 -0.028486675701486774 1104 32-33 0.05317473893219926 1104 34-35 0.04750263521392384 1104 36-37 -0.05124027234992212 1104 38-39 -0.049294962657334906 1104 40-41 0.04265985185610788 1104 42-43 -0.027597531308401813 1104 44-45 0.11835858563618018 1104 46-47 -0.013440706275375192 1104 48-49 0.07330339062860958 1104 50-51 -0.14052855994542313 1104 52-53 -0.06097563014815677 1104 54-55 0.14919603492054279 1104 56-57 0.09119961085563233 1104 58-59 0.12346974155532564 1104 60-61 -0.05116430157760021 1104 62-63 -0.3307886050081592 1104 64-65 -0.1759332812862695 1104 66-67 -0.15518299751249032 1104 68-69 -0.09774637091696547 1104 70-71 -0.17057914884638947 1104 72-73 -0.13357444135739271 1104 74-75 -0.16843408678100857 1104 76-77 0.016827190080196175 1104 78-79 -0.024676457261371354 1104 80-81 0.10508492383730683 1104 82-83 0.0714718803356007 1104 84-85 0.13404313290444492 1104 86-87 0.13399064440593378 1104 88-89 0.14413235856636675 1104 90-91 -0.03918857072059012 1104 92-93 -0.016685467690578548 1104 94-95 -0.04262107321364539 1104 96-97 -0.10140941954561455 1104 98-99 -0.3049910489595895 1104 100-101 -0.3187611182525387 1105 1 0.3100181297867266 1105 2 0.3667567278328825 1105 3 0.12238874867009031 1105 4 0.05656728926269494 1105 5 -0.12124496199579227 1105 6 0.05433586243265154 1105 7 0.096841975307278 1105 8 0.05772422455669357 1105 9 0.10877150192574447 1105 10-11 0.06912499109742498 1105 12-13 4.8325842945473596E-4 1105 14-15 0.1169199327304824 1105 16-17 0.06315746538421507 1105 18-19 0.0752583518093104 1105 20-21 0.0683085026392547 1105 22-23 0.08287381283869877 1105 24-25 -0.016406422345994542 1105 26-27 0.0620675848118708 1105 28-29 0.18105734658842465 1105 30-31 0.14423876660313795 1105 32-33 0.12230386982360386 1105 34-35 0.09618776167773291 1105 36-37 0.16703171944242712 1105 38-39 0.05732432856254377 1105 40-41 0.08767723443265396 1105 42-43 0.1823648705225338 1105 44-45 -0.04666434155732446 1105 46-47 0.13289746881714848 1105 48-49 0.09600495465578263 1105 50-51 -0.017715294145794758 1105 52-53 -0.07366880642447882 1105 54-55 -0.030676954902865816 1105 56-57 0.006011073642959275 1105 58-59 0.0939820924931638 1105 60-61 -0.035761294938225774 1105 62-63 -0.10088075897043325 1105 64-65 -0.06787547561901164 1105 66-67 -0.049471536141673766 1105 68-69 -0.18128366453680655 1105 70-71 -0.2578911832958646 1105 72-73 -0.2834847460482628 1105 74-75 -0.43028216427613586 1105 76-77 -0.31616398912223076 1105 78-79 -0.3474852205891992 1105 80-81 -0.4038937131987126 1105 82-83 -0.15387278641065905 1105 84-85 0.10025511737142168 1105 86-87 -0.11486249002269844 1105 88-89 -0.031178716704843623 1105 90-91 -0.17992597442334102 1105 92-93 -0.030184029934975598 1105 94-95 0.09353645663926002 1105 96-97 -0.05700391782860237 1105 98-99 -0.06644402190631027 1105 100-101 0.22373057999888246 1106 1 0.31647279362636027 1106 2 0.34116426828920865 1106 3 0.12407910565729452 1106 4 0.05690099709183016 1106 5 -0.06885743296886204 1106 6 -0.04038222213961262 1106 7 -0.023999557240998115 1106 8 0.028132791240167876 1106 9 -0.009453861131028418 1106 10-11 -0.0749521483785145 1106 12-13 -0.06807405125719157 1106 14-15 0.022473191133109083 1106 16-17 0.0700747726104396 1106 18-19 -0.07747455627765021 1106 20-21 -0.027139559594694163 1106 22-23 -0.0034326395119279596 1106 24-25 -0.05121678850195366 1106 26-27 -0.08650790728560054 1106 28-29 -0.08837282133535496 1106 30-31 -0.13182303939892392 1106 32-33 -0.14261335644003026 1106 34-35 -0.29731955097929585 1106 36-37 -0.15738013200908796 1106 38-39 -0.06639722478364973 1106 40-41 -0.06103422487601762 1106 42-43 -0.1521383897990063 1106 44-45 -0.07289339577230436 1106 46-47 -0.1396887287431383 1106 48-49 -0.2520581598910425 1106 50-51 -0.2154708297002017 1106 52-53 0.01277637322573355 1106 54-55 -0.1090066861018002 1106 56-57 -0.18100482656907246 1106 58-59 -0.14456676080840225 1106 60-61 -0.03250695358205746 1106 62-63 -0.4575429945999758 1106 64-65 -0.21239068055163557 1106 66-67 -0.2227258985270879 1106 68-69 -0.23422807425379233 1106 70-71 -0.2796818182371119 1106 72-73 -0.14407235309368716 1106 74-75 -0.013909162251547258 1106 76-77 -0.11832302226063973 1106 78-79 -0.13958820027546892 1106 80-81 -0.1731494041066668 1106 82-83 -0.16265630768410944 1106 84-85 -0.055418862039896766 1106 86-87 -0.059630887063249816 1106 88-89 0.011970862210816335 1106 90-91 -0.06063048566952034 1106 92-93 0.19276749818138939 1106 94-95 0.11199500773480509 1106 96-97 0.20273794556226932 1106 98-99 0.2620389599144346 1106 100-101 0.25514380308289475 1107 1 0.6251118944672136 1107 2 0.5818498576814157 1107 3 0.3967599476137309 1107 4 0.24176935627555451 1107 5 0.10803221833457144 1107 6 0.25401019363717836 1107 7 0.19405950682668305 1107 8 0.2546744918209498 1107 9 0.37739048848000323 1107 10-11 0.3786684199518078 1107 12-13 0.41574461874738233 1107 14-15 0.6143929841137847 1107 16-17 0.5653003406651251 1107 18-19 0.4989445670681292 1107 20-21 0.3894630080416732 1107 22-23 0.5036890005585093 1107 24-25 0.46056092189797937 1107 26-27 0.3729479697739748 1107 28-29 0.4501464262090451 1107 30-31 0.414523234804399 1107 32-33 0.5006456252609439 1107 34-35 0.41932103829940814 1107 36-37 0.5396923636699 1107 38-39 0.5350214121445234 1107 40-41 0.4946959365821968 1107 42-43 0.39519101544198065 1107 44-45 0.3632669696245969 1107 46-47 0.4938114998731322 1107 48-49 0.48857043757244867 1107 50-51 0.3691354314671358 1107 52-53 0.29271334971878815 1107 54-55 0.3676337572516317 1107 56-57 0.33984222103824635 1107 58-59 0.2840720481818906 1107 60-61 0.28371312768210544 1107 62-63 0.26005072996444056 1107 64-65 0.35224457110461316 1107 66-67 0.3851633085149402 1107 68-69 0.2529953568062453 1107 70-71 0.25746889339183454 1107 72-73 0.30164717203302516 1107 74-75 0.4796237536273935 1107 76-77 0.3796356452145595 1107 78-79 0.5700624870325939 1107 80-81 0.6416383394669474 1107 82-83 0.6143341942015716 1107 84-85 0.6234626693324188 1107 86-87 1.0095966331473996 1107 88-89 0.942491945170957 1107 90-91 0.7776209079169014 1107 92-93 0.9600367011375113 1107 94-95 1.048656058980523 1107 96-97 1.0223208748987638 1107 98-99 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-1.6771825737109616 2316 96-97 -1.7738031819637214 2316 98-99 -2.099667888729666 2316 100-101 -2.733323941773058 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 12.0 4 27.0 5 72.0 6 155.0 7 406.0 8 923.0 9 1689.0 10 2832.0 11 4322.0 12 6177.0 13 8337.0 14 10509.0 15 13628.0 16 17339.0 17 21488.0 18 19555.0 19 14484.0 20 13995.0 21 14342.0 22 15622.0 23 17606.0 24 21053.0 25 25485.0 26 31489.0 27 39438.0 28 48412.0 29 61213.0 30 75297.0 31 93113.0 32 116494.0 33 148704.0 34 194906.0 35 263973.0 36 374678.0 37 494561.0 38 310969.0 39 19076.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.70184002700839 19.158305966882423 14.935095878152122 23.20475812795707 2 15.794269600008631 20.21758270656709 39.38310135088958 24.6050463425347 3 19.90487064381761 23.576686354068716 29.408095106976567 27.11034789513711 4 13.558406865311584 16.750063746809662 36.171298668391096 33.52023071948766 5 14.356092802592086 36.81896525197742 33.79733030902094 15.027611636409558 6 33.02119136648328 35.72664819630217 16.789289586302996 14.46287085091155 7 29.606451759859688 31.97910634925773 19.854163244933737 18.56027864594885 8 26.728719418699026 34.963137196604684 18.92872685160209 19.3794165330942 9 26.107313673333678 16.9626643306563 19.9654154304568 36.964606565553225 10-11 25.589608456905644 26.266503781798217 26.719352488689772 21.42453527260637 12-13 26.360137700661106 23.409272236141167 27.379245675474735 22.851344387722992 14-15 22.987731314141257 25.227213056973202 25.605152200678795 26.179903428206746 16-17 22.68510026626577 28.413271008840756 26.278189584584638 22.623439140308832 18-19 22.50098056395365 26.94118194771271 28.544891047006526 22.01294644132711 20-21 23.21173743309888 25.941371178798484 28.773367189007935 22.073524199094706 22-23 22.889168759200103 25.840754858583903 28.660045289436987 22.610031092779007 24-25 22.98305577834284 26.14134024833089 28.624149661902816 22.25145431142345 26-27 22.751137214187896 26.282245711794378 28.689667105847338 22.27694996817039 28-29 22.435938166238873 26.575605611761887 28.669666202709653 22.318790019289583 30-31 22.693741093500364 26.106863609371217 28.58953414860857 22.60986114851985 32-33 22.591996623914774 26.26892429044963 28.651466157323252 22.487612928312338 34-35 23.14946042184384 26.296395239475835 28.293740557706958 22.260403780973366 36-37 23.082753054816962 26.03707321829735 28.404717465022937 22.475456261862746 38-39 22.777219643348104 26.37748642894136 28.521741667146213 22.323552260564323 40-41 22.992070507819992 26.219516093967798 28.381183900916916 22.40722949729529 42-43 22.844964880553917 26.370058049496937 27.97614075754094 22.808836312408204 44-45 22.970481529651046 26.353643387401878 27.963251321708388 22.712623761238692 46-47 23.196658337820786 26.715414226041744 27.47937686512365 22.60855057101382 48-49 23.327750175048198 26.679723343916155 27.402816277723957 22.58971020331169 50-51 23.069176599218824 26.825563523975994 27.392064668087713 22.71319520871747 52-53 23.385203387385342 27.00876738320846 26.954937832263603 22.651091397142597 54-55 23.061465749384336 26.783049460189417 27.150370038214767 23.00511475221148 56-57 23.14910282540063 27.015046157535068 27.085920952723498 22.749930064340806 58-59 23.407565805774063 26.76001572912654 27.056375491335395 22.776042973764007 60-61 23.26279599147653 26.81267554074943 27.493917518027644 22.43061094974639 62-63 23.058655355527208 26.85966018625975 27.438345018526867 22.643339439686176 64-65 22.880883769449362 26.96094340150519 27.230786447696055 22.927386381349397 66-67 23.03816842702601 27.026051719797152 26.966627103539505 22.969152749637338 68-69 22.922691752068527 26.772439093105177 27.260593393902216 23.04427576092408 70-71 23.086930528947406 26.869910316876112 27.10998311198282 22.93317604219367 72-73 23.161185699893743 26.74440444263817 27.18106751673502 22.913342340733067 74-75 22.960876954683666 27.255136795387763 26.83104172136444 22.952944528564124 76-77 23.08558412300376 27.00040461763633 26.86561199666315 23.04839926269675 78-79 23.091113895816793 26.99378277044289 26.894108868759165 23.020994464981147 80-81 23.1382003992718 27.251978873366095 26.750251293436794 22.859569433925316 82-83 23.173067027691324 27.18363798951896 26.817071403949623 22.82622357884009 84-85 23.095664955647244 27.202611129672466 26.62102571644609 23.080698198234202 86-87 23.01801402147423 27.11810825997677 26.79159063242108 23.072287086127915 88-89 22.98944646135182 27.19049631910239 26.504861063228297 23.315196156317484 90-91 23.06212329209261 27.145052941706666 26.679858209237107 23.112965556963623 92-93 23.222802793402135 26.90030271847182 26.833644710419307 23.043249777706734 94-95 22.94580180280992 26.92277411323213 26.872778209619113 23.258645874338832 96-97 22.924437870567406 26.87834248775907 26.995840992550633 23.201378649122887 98-99 22.781885323539168 26.966772841173864 26.5756922990844 23.675649536202574 100-101 22.492696767634015 27.24935460387895 26.619155440800707 23.63879318768633 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 265.0 1 188.0 2 149.0 3 199.5 4 198.0 5 205.0 6 317.0 7 636.5 8 1028.0 9 1343.5 10 1444.0 11 1415.5 12 1522.5 13 1742.5 14 2041.0 15 2296.5 16 2527.0 17 2753.5 18 2916.0 19 3173.5 20 3520.0 21 4005.0 22 4727.5 23 5639.0 24 6868.0 25 8563.0 26 10807.5 27 13554.0 28 16423.0 29 20114.0 30 24863.0 31 29779.0 32 35196.0 33 40093.0 34 45217.0 35 50216.0 36 55883.0 37 61766.0 38 65368.0 39 67556.0 40 69252.0 41 73363.0 42 78134.5 43 82129.0 44 85991.0 45 89227.0 46 90956.0 47 95077.5 48 105276.0 49 105914.0 50 98071.0 51 93536.5 52 90304.5 53 89502.5 54 88202.5 55 83249.0 56 76264.5 57 69158.5 58 63115.5 59 56073.0 60 46738.5 61 37945.5 62 31037.0 63 24724.0 64 18998.0 65 14750.5 66 11556.5 67 9556.0 68 7405.5 69 5142.0 70 3733.0 71 2817.5 72 2252.5 73 1865.0 74 1522.0 75 1172.5 76 748.0 77 432.0 78 283.0 79 192.0 80 122.5 81 73.0 82 45.5 83 30.0 84 18.0 85 14.0 86 13.5 87 7.5 88 3.0 89 2.0 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0437634146916523 2 0.010310155863181833 3 0.0043958028874031075 4 0.01202851517371214 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004595612109557794 10-11 2.3977106658562406E-4 12-13 9.990461107734335E-5 14-15 0.0 16-17 0.0 18-19 1.9980922215468672E-5 20-21 0.0 22-23 0.0014985691661601503 24-25 0.0 26-27 0.0 28-29 0.0 30-31 3.9961844430937344E-5 32-33 0.0032169284766904557 34-35 6.194085886795288E-4 36-37 0.002737386343519208 38-39 1.1988553329281203E-4 40-41 0.003316833087767799 42-43 0.008471911019358716 44-45 0.0015385310105910876 46-47 0.001578492855022025 48-49 0.008751643930375278 50-51 3.5965659987843607E-4 52-53 0.001758321154961243 54-55 0.008511872863789655 56-57 0.0034766804654915483 58-59 9.191224219115588E-4 60-61 0.004755459487281543 62-63 0.0029971383323203005 64-65 0.015065615350463376 66-67 0.002277825132563429 68-69 7.992368886187469E-5 70-71 8.391987330496841E-4 72-73 0.0 74-75 0.0 76-77 9.79065188557965E-4 78-79 0.009091319608038245 80-81 0.013267332351071196 82-83 0.009970480185518868 84-85 0.006993322775414035 86-87 0.008991414996960902 88-89 0.02475636262496568 90-91 0.021239720315043197 92-93 0.0024776343547181154 94-95 0.007273055686430596 96-97 0.009091319608038245 98-99 0.008671720241513402 100-101 0.009650785430071368 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2502387.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.64219213422216 #Duplication Level Percentage of deduplicated Percentage of total 1 78.2547951748782 45.1077793889453 2 12.790515579187728 14.745467130226015 3 3.8432168000573648 6.645943236071313 4 1.669981937539572 3.850456788173463 5 0.8875525078989067 2.5580236094759754 6 0.5637715867150899 1.9498218072747895 7 0.3772995878366822 1.5223862733971405 8 0.26181147212066963 1.207310974313855 9 0.18423890423870187 0.9557940885053228 >10 1.0326697038080723 10.918285314905123 >50 0.08611732957521707 3.4307095348627055 >100 0.0447587802176841 4.7251255448352865 >500 0.0022964039481348695 0.9259063983608201 >1k 7.654679827097513E-4 0.7299189330217372 >5k 2.0876399528447758E-4 0.7270709776310386 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6815 0.272339969796838 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6101 0.24380721287314872 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5210 0.20820120948518353 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3624 0.14482172421771694 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.9961844430937344E-5 0.0 0.0 0.0 6 0.0 3.9961844430937344E-5 0.0 0.0 0.0 7 0.0 3.9961844430937344E-5 0.0 0.0 0.0 8 0.0 3.9961844430937344E-5 0.0 0.0 0.0 9 0.0 3.9961844430937344E-5 0.0 0.0 0.0 10-11 0.0 3.9961844430937344E-5 0.0 0.0 0.0 12-13 0.0 3.9961844430937344E-5 0.0 0.0 0.0 14-15 0.0 3.9961844430937344E-5 0.0 0.0 0.0 16-17 0.0 3.9961844430937344E-5 0.0 0.0 0.0 18-19 0.0 3.9961844430937344E-5 0.0 0.0 0.0 20-21 0.0 3.9961844430937344E-5 0.0 1.9980922215468672E-5 0.0 22-23 0.0 3.9961844430937344E-5 0.0 1.1988553329281203E-4 0.0 24-25 0.0 3.9961844430937344E-5 0.0 2.597519888010927E-4 0.0 26-27 0.0 3.9961844430937344E-5 0.0 5.994276664640602E-4 0.0 28-29 0.0 3.9961844430937344E-5 0.0 0.0012388171773590575 0.0 30-31 0.0 3.9961844430937344E-5 0.0 0.0024976152769335836 0.0 32-33 0.0 3.9961844430937344E-5 0.0 0.0041360508986020145 0.0 34-35 0.0 3.9961844430937344E-5 0.0 0.005734524675839509 0.0 36-37 0.0 3.9961844430937344E-5 0.0 0.007392941219723408 0.0 38-39 0.0 3.9961844430937344E-5 0.0 0.010350117707612771 0.0 40-41 0.0 3.9961844430937344E-5 0.0 0.014426225839568381 0.0 42-43 0.0 3.9961844430937344E-5 0.0 0.018222601060507427 0.0 44-45 0.0 3.9961844430937344E-5 0.0 0.02056036895971726 0.0 46-47 3.9961844430937344E-5 3.9961844430937344E-5 0.0 0.024017068502993344 0.0 48-49 3.9961844430937344E-5 3.9961844430937344E-5 0.0 0.02837290954596551 0.0 50-51 3.9961844430937344E-5 3.9961844430937344E-5 0.0 0.03304844534438518 0.0 52-53 3.9961844430937344E-5 3.9961844430937344E-5 0.0 0.041840051119191396 0.0 54-55 3.9961844430937344E-5 3.9961844430937344E-5 0.0 0.04779436593940106 0.0 56-57 3.9961844430937344E-5 3.9961844430937344E-5 0.0 0.06507786365578147 0.0 58-59 3.9961844430937344E-5 3.9961844430937344E-5 0.0 0.09430995285701213 0.0 60-61 3.9961844430937344E-5 3.9961844430937344E-5 0.0 0.116388871905105 0.0 62-63 3.9961844430937344E-5 3.9961844430937344E-5 0.0 0.12983603255611542 0.0 64-65 3.9961844430937344E-5 3.9961844430937344E-5 0.0 0.15756955259118594 0.0 66-67 3.9961844430937344E-5 3.9961844430937344E-5 0.0 0.21311651635018886 1.9980922215468672E-5 68-69 3.9961844430937344E-5 3.9961844430937344E-5 0.0 0.2872057759251467 3.9961844430937344E-5 70-71 3.9961844430937344E-5 5.9942766646406016E-5 0.0 0.3516842119144641 7.992368886187469E-5 72-73 3.9961844430937344E-5 7.992368886187469E-5 0.0 0.4230161042236872 7.992368886187469E-5 74-75 3.9961844430937344E-5 7.992368886187469E-5 0.0 0.5129702160377272 7.992368886187469E-5 76-77 3.9961844430937344E-5 7.992368886187469E-5 0.0 0.5711746424513874 7.992368886187469E-5 78-79 3.9961844430937344E-5 7.992368886187469E-5 0.0 0.648920410791776 7.992368886187469E-5 80-81 3.9961844430937344E-5 7.992368886187469E-5 0.0 0.7551589742114229 7.992368886187469E-5 82-83 3.9961844430937344E-5 7.992368886187469E-5 0.0 0.8510074580790261 7.992368886187469E-5 84-85 3.9961844430937344E-5 7.992368886187469E-5 0.0 0.9839605145007546 7.992368886187469E-5 86-87 3.9961844430937344E-5 7.992368886187469E-5 0.0 1.1908629640419328 7.992368886187469E-5 88-89 3.9961844430937344E-5 7.992368886187469E-5 0.0 1.4588271118735832 7.992368886187469E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8235 0.0 24.775034 1 GTATCAA 14685 0.0 23.079144 1 TACATGG 8820 0.0 22.298428 2 ACATGGG 8670 0.0 21.58577 3 GAGTACT 8620 0.0 21.017138 12-13 GTGTAGC 1270 0.0 20.789684 1 GGTATCA 11270 0.0 20.488497 1 GTACTTT 8955 0.0 20.2309 14-15 AGTACTT 9115 0.0 19.875778 12-13 AGAGTAC 13155 0.0 19.854856 10-11 TCAACGC 17175 0.0 19.440805 4 ATCAACG 17215 0.0 19.397186 3 TATCAAC 17395 0.0 19.308008 2 CAACGCA 17385 0.0 19.231756 5 AACGCAG 17700 0.0 19.023655 6 GGACCGT 355 7.8216544E-11 18.729261 6 CATGGGG 5415 0.0 17.893175 4 TACCTGG 1915 0.0 17.86102 2 GTACCTG 2090 0.0 17.686152 1 ACTTTTT 10600 0.0 17.584448 16-17 >>END_MODULE