FastQCFastQC Report
Thu 26 May 2016
SRR936625_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936625_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2572625
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57670.22416792186968565No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47140.18323696613381274No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40270.15653272435741705No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28680.11148146348573928No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26960.10479568534084835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA123800.025.4106671
GTACATG87900.024.327771
GGTATCA90200.022.6537761
TACATGG91400.022.0452612
GTATAGG8850.022.015031
GTACCGT2606.220944E-1021.9324236
ACATGGG90450.021.9090273
GAGTACT66400.021.9006712-13
TCAACGC142350.021.734244
ATCAACG142700.021.6796673
TATCAAC144300.021.5710142
CAACGCA144250.021.4133535
AACGCAG147100.021.1272896
GTACTTT71850.020.13984514-15
AGAGTAC115250.019.29653710-11
ACGCAGA162900.018.9333577
CGCAGAG164700.018.6968548
AGTACTT73150.018.35304512-13
CATGGGG54150.018.3429324
GCAGAGT172200.017.495849
Produced by FastQC (version 0.11.5)