##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936625_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2572625 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.185044458481123 31.0 31.0 34.0 28.0 34.0 2 31.23762149555415 31.0 31.0 34.0 28.0 34.0 3 31.28811972207376 31.0 31.0 34.0 27.0 34.0 4 34.908044507069626 37.0 35.0 37.0 32.0 37.0 5 34.86493445410816 37.0 35.0 37.0 32.0 37.0 6 34.984929012195714 37.0 35.0 37.0 32.0 37.0 7 34.83383742286575 37.0 35.0 37.0 32.0 37.0 8 34.77647422379865 37.0 35.0 37.0 32.0 37.0 9 36.227101890092804 39.0 35.0 39.0 32.0 39.0 10-11 36.12320023322482 39.0 35.0 39.0 31.0 39.0 12-13 36.00370516495797 39.0 35.0 39.0 30.0 39.0 14-15 37.04490627277586 39.0 36.0 41.0 31.0 41.0 16-17 36.90821903697585 39.0 36.0 41.0 31.0 41.0 18-19 36.8277263495457 39.0 36.0 41.0 30.0 41.0 20-21 36.722173266605125 39.0 36.0 41.0 30.0 41.0 22-23 36.58180885282542 39.0 36.0 40.5 30.0 41.0 24-25 36.726563918176964 39.0 36.0 41.0 30.0 41.0 26-27 36.711145814100384 39.0 36.0 41.0 30.0 41.0 28-29 36.60924911325981 39.0 36.0 41.0 30.0 41.0 30-31 36.43894562946407 39.0 36.0 41.0 30.0 41.0 32-33 36.279967542879355 39.0 35.0 40.5 30.0 41.0 34-35 36.06303561537341 39.0 35.0 40.0 29.0 41.0 36-37 35.87800476167339 38.0 35.0 40.0 28.0 41.0 38-39 35.67971332782663 38.0 35.0 40.0 27.0 41.0 40-41 35.50379709440746 38.0 34.5 40.0 27.0 41.0 42-43 35.28204440989262 38.0 34.0 40.0 26.0 41.0 44-45 35.04382372090763 38.0 34.0 40.0 25.5 41.0 46-47 34.808041008697344 38.0 34.0 40.0 24.5 41.0 48-49 34.738012535834024 38.0 34.0 40.0 24.0 41.0 50-51 33.833092075215006 37.5 32.5 39.5 23.5 40.5 52-53 33.98430707934503 37.5 33.0 39.5 23.5 40.5 54-55 34.400236917545314 38.0 33.5 40.0 24.0 41.0 56-57 34.300976240221566 38.0 33.5 40.0 23.5 41.0 58-59 34.04100383849182 38.0 33.0 40.0 23.0 41.0 60-61 33.70861610223021 37.0 33.0 40.0 22.0 41.0 62-63 33.36673184004665 37.0 32.5 40.0 22.0 41.0 64-65 33.01510849812934 36.0 32.0 39.0 20.5 41.0 66-67 32.61443914289879 36.0 31.5 39.0 19.5 41.0 68-69 32.20004198046742 35.0 31.0 39.0 18.5 40.0 70-71 31.763025703318593 35.0 31.0 38.0 17.0 40.0 72-73 31.35995316068218 35.0 31.0 37.0 14.0 39.5 74-75 30.91553763179632 35.0 30.0 37.0 11.0 39.0 76-77 30.48264282590739 34.5 30.0 36.0 7.5 39.0 78-79 30.025561051455227 34.0 29.0 36.0 7.0 38.5 80-81 29.545425586706184 34.0 29.0 35.0 3.5 37.0 82-83 29.0651791458141 34.0 29.0 35.0 2.0 37.0 84-85 28.734437976774696 34.0 28.5 35.0 2.0 36.0 86-87 28.404458869831398 34.0 28.0 35.0 2.0 36.0 88-89 27.993396239249794 34.0 27.0 35.0 2.0 35.5 90-91 27.576751955687286 33.0 26.0 35.0 2.0 35.0 92-93 27.17987289247364 33.0 25.5 35.0 2.0 35.0 94-95 26.692951945969583 33.0 25.0 35.0 2.0 35.0 96-97 26.081365531315292 33.0 24.5 35.0 2.0 35.0 98-99 25.327322287546764 32.0 21.0 35.0 2.0 35.0 100-101 22.78287507895632 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3609515817621052 1101 2 0.42548158217997667 1101 3 0.3736954854474206 1101 4 0.473792386423483 1101 5 0.3900904853563958 1101 6 0.275756949821897 1101 7 0.3439228022253005 1101 8 0.4110905198244552 1101 9 0.5617683853735116 1101 10-11 0.5059197686353016 1101 12-13 0.5588786177536846 1101 14-15 0.6312159914604081 1101 16-17 0.7319575766451152 1101 18-19 0.801847061710447 1101 20-21 0.7721441382748182 1101 22-23 0.7892213947489779 1101 24-25 0.7592544262695569 1101 26-27 0.6686730172912974 1101 28-29 0.6388433754785652 1101 30-31 0.7055395089671634 1101 32-33 0.5902028905151013 1101 34-35 0.42710527156950207 1101 36-37 0.5735458724278857 1101 38-39 0.6271197869100433 1101 40-41 0.5850174130981429 1101 42-43 0.5375066947620653 1101 44-45 0.37023293183967354 1101 46-47 0.40025953157123695 1101 48-49 0.5492229391270413 1101 50-51 0.4601150041537707 1101 52-53 0.5625447085837365 1101 54-55 0.56611996015004 1101 56-57 0.4535873495584468 1101 58-59 0.4028880245186599 1101 60-61 0.368927949311761 1101 62-63 0.36345873353111813 1101 64-65 0.5111610830418272 1101 66-67 0.5177976424520807 1101 68-69 0.5484702321233854 1101 70-71 0.5211317150253159 1101 72-73 0.5111695362504243 1101 74-75 0.5668951933957445 1101 76-77 0.40097980688389967 1101 78-79 0.3699934512119931 1101 80-81 0.5503575208762364 1101 82-83 0.6071700408150704 1101 84-85 0.6870623136583625 1101 86-87 0.6069574040805001 1101 88-89 0.5403164974031291 1101 90-91 0.3102997726395458 1101 92-93 0.37420280758982827 1101 94-95 0.5355514379384907 1101 96-97 0.6215884493164658 1101 98-99 0.7444640881093143 1101 100-101 1.0152210587867216 1102 1 0.33905385700702695 1102 2 0.45872375930266784 1102 3 0.3289912020376562 1102 4 0.25158551036467003 1102 5 0.4149974222428554 1102 6 0.3136757281490645 1102 7 0.31102381078652996 1102 8 0.20227879826569506 1102 9 0.4334427438783095 1102 10-11 0.4174183305861092 1102 12-13 0.3824765155339094 1102 14-15 0.45857506337900844 1102 16-17 0.3880249324340923 1102 18-19 0.5993144786794602 1102 20-21 0.6166257204347048 1102 22-23 0.4510832913278904 1102 24-25 0.32395152085432954 1102 26-27 0.390961682717311 1102 28-29 0.46219033506825014 1102 30-31 0.34463650888315556 1102 32-33 0.35300639357814134 1102 34-35 0.0696802236525258 1102 36-37 0.1587253208003787 1102 38-39 0.09620539542577689 1102 40-41 0.12996837187527888 1102 42-43 0.170855362891281 1102 44-45 -0.13080498034549493 1102 46-47 0.09496721955576959 1102 48-49 0.15495584517771022 1102 50-51 0.3525286794269107 1102 52-53 0.2406343204019663 1102 54-55 0.2851654128715282 1102 56-57 0.449320263566392 1102 58-59 0.4705980256815536 1102 60-61 0.3258173933907429 1102 62-63 0.3774551166109106 1102 64-65 0.4067739786843845 1102 66-67 0.5309520326995596 1102 68-69 0.31684285788718825 1102 70-71 0.32870708145475547 1102 72-73 0.42646860398804876 1102 74-75 0.27617269943275247 1102 76-77 0.4424773673554583 1102 78-79 0.17995327040917886 1102 80-81 0.32174004082312635 1102 82-83 0.3661223957520505 1102 84-85 0.3707751007751341 1102 86-87 0.4505734057865389 1102 88-89 0.4248719139338135 1102 90-91 0.44643484934251276 1102 92-93 0.20726570742133177 1102 94-95 0.42278311169224025 1102 96-97 0.2597305768426317 1102 98-99 0.36636690551713613 1102 100-101 0.4441000601993643 1103 1 0.13394151646938113 1103 2 0.27965335546317505 1103 3 0.31963533707680725 1103 4 0.3356702966176144 1103 5 0.32921876960597984 1103 6 0.23608634722632615 1103 7 0.3245166010388303 1103 8 0.17587588490707873 1103 9 0.47988375320750265 1103 10-11 0.4273433003168279 1103 12-13 0.39654859664447883 1103 14-15 0.33153525626003244 1103 16-17 0.5614771934996483 1103 18-19 0.44774508286214854 1103 20-21 0.5408344702388561 1103 22-23 0.5564493341367012 1103 24-25 0.5538510207860625 1103 26-27 0.49075124270918735 1103 28-29 0.17785552040297858 1103 30-31 0.17226563271817952 1103 32-33 0.2807949239739429 1103 34-35 0.10897975496656187 1103 36-37 0.22331387664996072 1103 38-39 0.2789580129929803 1103 40-41 0.3068409840816173 1103 42-43 0.26657231873413423 1103 44-45 0.3460759752973033 1103 46-47 0.4235530345464227 1103 48-49 0.45133725444478756 1103 50-51 0.336822932859306 1103 52-53 0.40566177076567556 1103 54-55 0.4828666874029679 1103 56-57 0.44703371562373917 1103 58-59 0.20145129828290465 1103 60-61 0.15689153243450704 1103 62-63 0.17903343383935066 1103 64-65 0.27078477731149064 1103 66-67 0.1532098146707952 1103 68-69 0.19290568751158332 1103 70-71 0.3264722651070677 1103 72-73 0.2763152461325653 1103 74-75 0.2042217190103024 1103 76-77 0.36367979045420995 1103 78-79 0.37333203827475003 1103 80-81 0.18522900270849973 1103 82-83 0.0666447017405396 1103 84-85 0.18987371893496174 1103 86-87 0.2579626575405207 1103 88-89 0.3256267374186308 1103 90-91 0.38791880447931604 1103 92-93 0.3350346210131967 1103 94-95 0.23455661611981427 1103 96-97 0.3607042409972223 1103 98-99 0.5953129189053215 1103 100-101 1.1255752392770937 1104 1 0.16794987916211213 1104 2 0.29035769686560897 1104 3 0.2911995773313798 1104 4 0.39120241200951256 1104 5 0.43845537793268363 1104 6 0.3069924871285892 1104 7 0.3790783173323504 1104 8 0.3258028415688159 1104 9 0.4500108036299011 1104 10-11 0.49165884753810474 1104 12-13 0.3390553800857745 1104 14-15 0.39224303081774536 1104 16-17 0.31728076492822055 1104 18-19 0.5668397598140729 1104 20-21 0.6942277890819071 1104 22-23 0.5302788057622649 1104 24-25 0.5139555070913175 1104 26-27 0.2543040375343608 1104 28-29 0.5350734334233991 1104 30-31 0.49991040532491837 1104 32-33 0.28172146155397115 1104 34-35 0.30920533694557406 1104 36-37 0.4281236088164917 1104 38-39 0.46299532073628313 1104 40-41 0.5511185061485264 1104 42-43 0.46169960592585113 1104 44-45 0.5956631087783748 1104 46-47 0.4785381535940445 1104 48-49 0.6354085977792607 1104 50-51 0.6733625372651062 1104 52-53 0.726371984047482 1104 54-55 0.7903165751743089 1104 56-57 0.6629416549729044 1104 58-59 0.6442783714351634 1104 60-61 0.8128532065830925 1104 62-63 0.8536997666612649 1104 64-65 0.7159921517290329 1104 66-67 0.8524845310211404 1104 68-69 0.6987815327780922 1104 70-71 0.7677664344255497 1104 72-73 0.6930025709232552 1104 74-75 0.5375088231319829 1104 76-77 0.7378674940758323 1104 78-79 0.6208410473477493 1104 80-81 0.6830866546197001 1104 82-83 0.6434401827611111 1104 84-85 0.7094919520675127 1104 86-87 0.7648147619463224 1104 88-89 0.784639243574631 1104 90-91 0.8330243088926998 1104 92-93 0.7404395912749742 1104 94-95 0.7535253909805952 1104 96-97 0.6974450993333967 1104 98-99 1.073733051874978 1104 100-101 1.4135513232070842 1105 1 0.4141879956208179 1105 2 0.29140493394225686 1105 3 0.3762151744793307 1105 4 0.375742667505655 1105 5 0.5458937320622184 1105 6 0.4580675762551465 1105 7 0.42192776771856444 1105 8 0.5505038962331099 1105 9 0.6033938992924064 1105 10-11 0.5838379919231329 1105 12-13 0.4103427018326613 1105 14-15 0.28496621857770066 1105 16-17 0.5079503282080751 1105 18-19 0.43999316890498363 1105 20-21 0.49838610013360096 1105 22-23 0.39534212275572855 1105 24-25 0.4257369242036191 1105 26-27 0.43104386225212465 1105 28-29 0.25906704601995756 1105 30-31 0.24415524341166162 1105 32-33 0.3420202212093457 1105 34-35 0.11260978584634529 1105 36-37 0.3700502279489939 1105 38-39 0.23753947551941224 1105 40-41 0.0937172880855428 1105 42-43 0.4540792850702431 1105 44-45 0.021674138190142855 1105 46-47 0.21924949494876245 1105 48-49 0.24154718957961308 1105 50-51 0.3953535874730676 1105 52-53 0.22535445582407476 1105 54-55 0.39170082205487944 1105 56-57 0.5535369461196709 1105 58-59 0.4348940874185274 1105 60-61 0.1908955788350184 1105 62-63 0.2986446196024417 1105 64-65 0.49220371590016043 1105 66-67 0.5940945651863245 1105 68-69 0.6487397547032216 1105 70-71 0.6696780134510938 1105 72-73 0.5169005581484569 1105 74-75 0.7154379303809435 1105 76-77 0.49304147654760655 1105 78-79 0.5869850238777552 1105 80-81 0.5135224463606392 1105 82-83 0.6058259893564788 1105 84-85 0.7104203537312124 1105 86-87 0.6886858255232688 1105 88-89 0.5607551823743968 1105 90-91 0.6369161686669109 1105 92-93 0.7416130909778822 1105 94-95 0.689721915044764 1105 96-97 0.8140365654653046 1105 98-99 0.879114067917758 1105 100-101 1.3020122189290113 1106 1 0.21647729170979702 1106 2 0.3002341273501479 1106 3 0.2779128108351543 1106 4 0.2820630361471643 1106 5 0.34406419621041806 1106 6 0.2403098416452636 1106 7 0.39619720987353446 1106 8 0.39483462673236147 1106 9 0.58348059098482 1106 10-11 0.38881918354979206 1106 12-13 0.5666032404461419 1106 14-15 0.7627961007796031 1106 16-17 0.7678582850158762 1106 18-19 0.7228671977249519 1106 20-21 0.591168606588603 1106 22-23 0.5260293282442063 1106 24-25 0.4867222772398492 1106 26-27 0.2725520997858055 1106 28-29 0.5877269315160945 1106 30-31 0.5223338845473862 1106 32-33 0.4469666476184102 1106 34-35 0.4258112587457532 1106 36-37 0.46466995245742737 1106 38-39 0.5072499216209536 1106 40-41 0.3385936502115072 1106 42-43 0.30038356015035106 1106 44-45 0.5189134283547077 1106 46-47 0.3473242961555201 1106 48-49 0.4088152814899928 1106 50-51 0.3061273544471561 1106 52-53 0.5239746542852899 1106 54-55 0.6445696035405106 1106 56-57 0.5369933248860619 1106 58-59 0.607736593170408 1106 60-61 0.6707156720891376 1106 62-63 0.8900717382950631 1106 64-65 0.6273937983080486 1106 66-67 0.7615017830704858 1106 68-69 0.5945855716142248 1106 70-71 0.7153651733671929 1106 72-73 0.9122297795508061 1106 74-75 0.6604839519770458 1106 76-77 0.7323128262323131 1106 78-79 0.8591332669321972 1106 80-81 0.9296358643023552 1106 82-83 1.0621540401996334 1106 84-85 0.8927390505173172 1106 86-87 1.0340603588946244 1106 88-89 1.1651833856897191 1106 90-91 1.2488712369017883 1106 92-93 1.5305804077671397 1106 94-95 1.5673113052732646 1106 96-97 1.7746695788805127 1106 98-99 2.0045721680181465 1106 100-101 2.2177191312178017 1107 1 0.23267374592446188 1107 2 0.3938964482111409 1107 3 0.21053264155814588 1107 4 0.12652638641448988 1107 5 0.2774406966744607 1107 6 0.34664421838083115 1107 7 0.3513061459413649 1107 8 0.43830919547385605 1107 9 0.4527295274667651 1107 10-11 0.32880900900205035 1107 12-13 0.3738065686397789 1107 14-15 0.22416617099236902 1107 16-17 0.1964149145514682 1107 18-19 0.35594711090843845 1107 20-21 0.47227710286016844 1107 22-23 0.6142196845447074 1107 24-25 0.5264342028214983 1107 26-27 0.4009622572449203 1107 28-29 0.48983288205395326 1107 30-31 0.5605750611675191 1107 32-33 0.3550587941098797 1107 34-35 0.528229863019007 1107 36-37 0.6692176765117424 1107 38-39 0.5189334187508763 1107 40-41 0.34007691423367703 1107 42-43 0.5216116977809051 1107 44-45 0.6139090946054253 1107 46-47 0.655266602643195 1107 48-49 0.6115949092252606 1107 50-51 0.5114928820205193 1107 52-53 0.6709076257171418 1107 54-55 0.6792131261251555 1107 56-57 0.5236121040818631 1107 58-59 0.7848738444647054 1107 60-61 0.7127050110921189 1107 62-63 0.7494899308162672 1107 64-65 0.7803764583311121 1107 66-67 0.791016293071138 1107 68-69 0.8186253388419615 1107 70-71 0.7549601388269345 1107 72-73 0.7386652832184382 1107 74-75 0.7037854721572181 1107 76-77 0.5651606931548905 1107 78-79 0.5804973010853409 1107 80-81 0.8670413624895694 1107 82-83 0.9096656267869498 1107 84-85 0.8129828622457218 1107 86-87 0.7033538122991523 1107 88-89 0.7072034553914008 1107 90-91 0.6689470454074673 1107 92-93 0.7298338024937259 1107 94-95 0.7288460763053131 1107 96-97 0.6179145781636812 1107 98-99 0.9156620974436898 1107 100-101 1.3686660298809308 1108 1 -0.10764055157699559 1108 2 0.11185182315048436 1108 3 0.04566910743417196 1108 4 0.18776690050682276 1108 5 0.2557675414177538 1108 6 0.15908958698174303 1108 7 -0.06346175853647651 1108 8 0.19584536481894332 1108 9 0.2254360361311214 1108 10-11 0.08954798116512563 1108 12-13 0.3503324542414248 1108 14-15 0.2766057939112372 1108 16-17 0.3509674389370261 1108 18-19 0.2439345652472582 1108 20-21 0.2551974109614932 1108 22-23 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96-97 -5.211752936224109 2316 98-99 -6.660897106158384 2316 100-101 -6.931081669915535 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7221.0 3 2963.0 4 2998.0 5 3313.0 6 3669.0 7 4254.0 8 5243.0 9 6427.0 10 8116.0 11 9618.0 12 11446.0 13 13049.0 14 14439.0 15 16627.0 16 18980.0 17 22794.0 18 20484.0 19 17455.0 20 18360.0 21 19971.0 22 22406.0 23 25519.0 24 29240.0 25 33448.0 26 38723.0 27 45903.0 28 53580.0 29 64554.0 30 76484.0 31 93893.0 32 115510.0 33 146261.0 34 189195.0 35 252633.0 36 346344.0 37 441099.0 38 326281.0 39 44113.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.268542014516164 18.0588812149218 14.23281542480346 23.439761345758576 2 15.609041090031821 19.24246754029397 40.17184836131701 24.976643008357197 3 19.740520281329825 23.207261077653406 29.126423464369168 27.925795176647604 4 13.056263149911912 15.95929701202889 36.688069505427194 34.296370332632 5 14.395873092298878 36.47820511873144 34.02890375513909 15.097018033830595 6 33.06629094061275 35.042780580210525 17.277124280103617 14.613804199073105 7 29.66498636129236 31.648763027163078 20.112640869143853 18.573609742400706 8 26.70147356370024 34.34951532575468 19.386736487860688 19.562274622684395 9 26.234952456060938 16.957701215936435 20.229545528785355 36.57780079921727 10-11 25.510109632833068 25.89491348767749 27.096655012425575 21.498321867063872 12-13 26.289417920077028 23.122042768849184 27.55362633781472 23.03491297325907 14-15 23.05183530636918 24.905010019104935 25.69940274235326 26.343751932172623 16-17 22.780600488837816 28.085015779311313 26.37427787532497 22.760105856525907 18-19 22.607445393750115 26.80686712471092 28.365595617304823 22.220091864234142 20-21 23.310199246012115 25.85371779782667 28.4027566527914 22.433326303369814 22-23 22.94204998280591 25.706984300210195 28.38084533501652 22.970120381967373 24-25 23.011612113729676 25.996759554765315 28.333643020797783 22.65798531070723 26-27 22.76889052766494 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27.496849996880606 23.037246321012088 52-53 23.23636572838627 26.676094432629505 27.085902937288125 23.001636901696106 54-55 22.949355224811242 26.43766810356054 27.3144208607602 23.29855581086802 56-57 22.97080951462712 26.746340109353522 27.190219453481212 23.092630922538145 58-59 23.222458083814296 26.4436760639186 27.23586519878299 23.098000653484117 60-61 23.165536033855037 26.467576032230085 27.664347348437506 22.702540585477376 62-63 22.904842473121935 26.610229941914426 27.530405763270906 22.954521821692733 64-65 22.75876692435428 26.650163412920037 27.34097559534545 23.250094067380232 66-67 22.80539918778166 26.822233650966915 27.04569670832716 23.32667045292426 68-69 22.676308000695126 26.54676619797379 27.465205706112577 23.311720095218508 70-71 22.900503411145017 26.49091333164882 27.27092849229334 23.337654764912827 72-73 22.908713709881965 26.440307562595006 27.440910272086118 23.21006845543691 74-75 22.710477188201832 26.90776641947042 27.101662087818646 23.280094304509106 76-77 22.880800829098042 26.6489635885702 27.122600618713637 23.347634963618113 78-79 22.82433216726538 26.715466850252074 27.196950937681123 23.263250044801424 80-81 22.947105502291205 26.892291544169456 27.03311676636522 23.127486187174117 82-83 22.981839456009734 26.79768199660055 27.125945952775005 23.094532594614712 84-85 22.903682865474263 26.80350307742938 26.94703804590281 23.34577601119355 86-87 22.880476200564466 26.737594262767445 27.130029023962603 23.25190051270549 88-89 22.832456844668016 26.73205880472175 26.91688041950172 23.518603931108515 90-91 22.844185106800232 26.690058625018537 27.09977098478486 23.365985283396366 92-93 23.08260809812879 26.465494769773013 27.22558001159702 23.22631712050117 94-95 22.75330044117324 26.49020125795699 27.28577867941923 23.470719621450538 96-97 22.698227428357065 26.458559492767918 27.389638245329976 23.453574833545034 98-99 22.594896820981283 26.57097379827945 27.045589119791234 23.788540260948032 100-101 22.54828440812727 26.436156985546518 27.19247677621897 23.823081830107242 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 139.0 1 114.5 2 124.5 3 174.0 4 168.5 5 158.5 6 272.5 7 568.0 8 966.0 9 1256.0 10 1296.0 11 1252.5 12 1325.0 13 1528.5 14 1695.0 15 1877.5 16 2105.0 17 2236.5 18 2401.5 19 2668.0 20 3003.5 21 3474.5 22 4221.0 23 5147.0 24 6236.0 25 7899.0 26 10343.5 27 13214.0 28 16162.0 29 19887.0 30 24547.5 31 29712.5 32 35471.5 33 40759.5 34 46038.5 35 51115.5 36 57040.0 37 62945.0 38 67042.0 39 69586.5 40 71411.0 41 75751.0 42 80251.0 43 84302.0 44 88508.5 45 92507.0 46 94903.0 47 98420.5 48 108120.5 49 109377.0 50 101366.0 51 96987.5 52 94329.5 53 93249.0 54 91954.5 55 86921.5 56 79630.5 57 72076.0 58 65413.5 59 57761.0 60 48192.5 61 39302.5 62 32411.0 63 25869.5 64 19946.5 65 15623.0 66 12201.0 67 10074.0 68 7834.5 69 5510.0 70 4071.0 71 3103.5 72 2418.5 73 1928.0 74 1488.0 75 1133.5 76 792.5 77 493.5 78 303.0 79 178.5 80 112.5 81 77.5 82 53.5 83 41.0 84 29.0 85 22.5 86 19.0 87 11.5 88 8.5 89 6.5 90 6.5 91 3.5 92 1.0 93 2.5 94 3.0 95 1.5 96 3.0 97 2.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03385646955930227 2 0.02293377386910257 3 0.021573295758223605 4 0.051115106165881156 5 0.05449686604149458 6 0.04851076235362713 7 0.04924930761381857 8 0.042913366697439385 9 0.043107720713279236 10-11 0.051115106165881156 12-13 0.04563432291919732 14-15 0.040347893688353335 16-17 0.04742237986492396 18-19 0.051717603614984696 20-21 0.011758417958311063 22-23 0.020232253048928624 24-25 0.0054030416403478936 26-27 0.009503911374568776 28-29 0.005480783246683835 30-31 0.008337787279529663 32-33 0.0033623244740294445 34-35 0.007132792381322579 36-37 0.008532141295369515 38-39 0.01107817890287158 40-41 0.0016325737330547595 42-43 0.0016714445362227296 44-45 0.0020990233710704046 46-47 4.8588503959963073E-4 48-49 0.001010640882367232 50-51 3.304018269277489E-4 52-53 0.0031874058597735773 54-55 0.006919002963898741 56-57 0.01620912492104368 58-59 0.010086973422088334 60-61 0.009542782177736747 62-63 0.004956027403916234 64-65 3.887080316797046E-5 66-67 0.006374811719547155 68-69 0.016947670181235122 70-71 0.0068023905543948306 72-73 0.0029347456391817695 74-75 0.0041786113405568245 76-77 0.0074243234050823574 78-79 0.006297070113211214 80-81 0.011175355910791506 82-83 0.005927797483115495 84-85 0.01669500996064331 86-87 0.0037121617025411787 88-89 0.005616831057771731 90-91 0.0034400660803653857 92-93 0.016073077109955782 94-95 0.010864389485447743 96-97 0.009037461736553131 98-99 0.021067975317039987 100-101 0.0373742772460036 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2572625.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.78381724256986 #Duplication Level Percentage of deduplicated Percentage of total 1 79.8577991523283 47.74204069916645 2 11.833235560393627 14.148719842617025 3 3.616084220575185 6.485499545298226 4 1.5277118717525577 3.6532978936063687 5 0.8249242914643983 2.4658561539932005 6 0.5083679076985248 1.8235304451501737 7 0.3433341289150568 1.4368077371336274 8 0.2404673989230109 1.1500847224007538 9 0.1758064056577634 0.9459340223325143 >10 0.9492388571786515 10.445652575738558 >50 0.07925506800405778 3.2839220838637884 >100 0.041163327137109525 4.489846852967873 >500 0.001762971730966533 0.7272795763892814 >1k 7.835429915326636E-4 0.9764072805585232 >5k 6.529524929438863E-5 0.22512056878347275 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5767 0.22416792186968565 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4714 0.18323696613381274 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4027 0.15653272435741705 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2868 0.11148146348573928 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2696 0.10479568534084835 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.887080316797046E-5 0.0 18-19 0.0 0.0 0.0 7.774160633594091E-5 0.0 20-21 0.0 0.0 0.0 1.1661240950391137E-4 0.0 22-23 0.0 0.0 0.0 1.7491861425586707E-4 0.0 24-25 0.0 0.0 0.0 3.304018269277489E-4 0.0 26-27 0.0 1.943540158398523E-5 0.0 4.664496380156455E-4 0.0 28-29 0.0 3.887080316797046E-5 0.0 9.523346776152762E-4 0.0 30-31 0.0 3.887080316797046E-5 0.0 0.0019629755599825083 0.0 32-33 0.0 3.887080316797046E-5 0.0 0.003614984694621253 0.0 34-35 3.887080316797046E-5 3.887080316797046E-5 0.0 0.005325300034011953 0.0 36-37 3.887080316797046E-5 3.887080316797046E-5 0.0 0.007424323405082358 0.0 38-39 3.887080316797046E-5 3.887080316797046E-5 0.0 0.011350274525047374 0.0 40-41 3.887080316797046E-5 3.887080316797046E-5 0.0 0.015937029298867887 0.0 42-43 3.887080316797046E-5 3.887080316797046E-5 0.0 0.020504348671104417 0.0 44-45 3.887080316797046E-5 3.887080316797046E-5 0.0 0.022856032262766626 0.0 46-47 3.887080316797046E-5 3.887080316797046E-5 0.0 0.02643214615421991 0.0 48-49 3.887080316797046E-5 3.887080316797046E-5 0.0 0.0309217239201205 0.0 50-51 3.887080316797046E-5 3.887080316797046E-5 0.0 0.036849521403235996 0.0 52-53 3.887080316797046E-5 3.887080316797046E-5 0.0 0.046003595549293036 0.0 54-55 3.887080316797046E-5 3.887080316797046E-5 0.0 0.0523589718672562 0.0 56-57 3.887080316797046E-5 3.887080316797046E-5 0.0 0.07088090957679413 0.0 58-59 3.887080316797046E-5 3.887080316797046E-5 0.0 0.09913998347990866 0.0 60-61 3.887080316797046E-5 7.774160633594091E-5 0.0 0.12419221612166562 0.0 62-63 3.887080316797046E-5 7.774160633594091E-5 0.0 0.13900199212866235 0.0 64-65 3.887080316797046E-5 7.774160633594091E-5 0.0 0.16885476896166368 0.0 66-67 3.887080316797046E-5 7.774160633594091E-5 0.0 0.2258004956027404 0.0 68-69 3.887080316797046E-5 7.774160633594091E-5 0.0 0.3046304844273845 0.0 70-71 3.887080316797046E-5 7.774160633594091E-5 0.0 0.37006948156066277 0.0 72-73 3.887080316797046E-5 7.774160633594091E-5 0.0 0.4445653758320781 0.0 74-75 3.887080316797046E-5 7.774160633594091E-5 0.0 0.5364365191195763 0.0 76-77 3.887080316797046E-5 7.774160633594091E-5 0.0 0.5975802925027939 0.0 78-79 3.887080316797046E-5 7.774160633594091E-5 0.0 0.6779651134541567 0.0 80-81 3.887080316797046E-5 7.774160633594091E-5 0.0 0.7835965210631164 0.0 82-83 3.887080316797046E-5 7.774160633594091E-5 0.0 0.8770224964773334 0.0 84-85 3.887080316797046E-5 7.774160633594091E-5 0.0 1.0174044021184587 0.0 86-87 3.887080316797046E-5 7.774160633594091E-5 0.0 1.2310189009280403 0.0 88-89 3.887080316797046E-5 7.774160633594091E-5 0.0 1.5012875953549392 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12380 0.0 25.410667 1 GTACATG 8790 0.0 24.32777 1 GGTATCA 9020 0.0 22.653776 1 TACATGG 9140 0.0 22.045261 2 GTATAGG 885 0.0 22.01503 1 GTACCGT 260 6.220944E-10 21.932423 6 ACATGGG 9045 0.0 21.909027 3 GAGTACT 6640 0.0 21.90067 12-13 TCAACGC 14235 0.0 21.73424 4 ATCAACG 14270 0.0 21.679667 3 TATCAAC 14430 0.0 21.571014 2 CAACGCA 14425 0.0 21.413353 5 AACGCAG 14710 0.0 21.127289 6 GTACTTT 7185 0.0 20.139845 14-15 AGAGTAC 11525 0.0 19.296537 10-11 ACGCAGA 16290 0.0 18.933357 7 CGCAGAG 16470 0.0 18.696854 8 AGTACTT 7315 0.0 18.353045 12-13 CATGGGG 5415 0.0 18.342932 4 GCAGAGT 17220 0.0 17.49584 9 >>END_MODULE