FastQCFastQC Report
Thu 26 May 2016
SRR936625_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936625_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2572625
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69520.2702298236237306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61150.23769496137213933No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51510.20022350711821585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36290.1410621446965648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG87200.023.2680741
GTATCAA145200.022.7112961
TACATGG88300.022.2179162
GGTATCA110600.021.6103551
ACATGGG87100.021.3770663
GAGTACT86350.020.93015712-13
TACCTGG21700.020.7959042
GTACCTG23300.020.3935641
GTGTAGC13700.020.1166881
TAAGGTG12300.020.080295
GTACTTT90500.019.76005614-15
TCAACGC168500.019.5064354
ATCAACG168600.019.43893
AGTACTT90850.019.42290112-13
AGAGTAC129550.019.39614110-11
CAACGCA169350.019.3804845
TATCAAC171600.019.2112732
AACGCAG171700.019.17136
CATGGGG55950.018.1670934
GTAAGGT9700.018.1176434
Produced by FastQC (version 0.11.5)