##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936625_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2572625 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.888471114134397 31.0 31.0 34.0 30.0 34.0 2 32.04639113745688 31.0 31.0 34.0 30.0 34.0 3 32.15848054030416 33.0 31.0 34.0 30.0 34.0 4 35.89077731888635 37.0 35.0 37.0 35.0 37.0 5 35.665238423788935 37.0 35.0 37.0 33.0 37.0 6 35.759908653612555 37.0 35.0 37.0 33.0 37.0 7 35.70826490452359 37.0 35.0 37.0 33.0 37.0 8 35.68066546815024 37.0 35.0 37.0 33.0 37.0 9 37.326031193819546 39.0 37.0 39.0 34.0 39.0 10-11 37.192397842670424 39.0 37.0 39.0 33.0 39.0 12-13 37.09690646712988 39.0 37.0 39.0 33.0 39.0 14-15 38.229251251153975 40.0 38.0 41.0 33.0 41.0 16-17 38.15480161313833 40.0 37.0 41.0 33.0 41.0 18-19 38.27772819590885 40.0 38.0 41.0 33.5 41.0 20-21 38.29939730819688 40.0 38.0 41.0 34.0 41.0 22-23 38.21042067926729 40.0 38.0 41.0 33.0 41.0 24-25 38.09927622564501 40.0 37.0 41.0 33.0 41.0 26-27 37.891690005344735 40.0 37.0 41.0 33.0 41.0 28-29 37.815249016082795 40.0 37.0 41.0 33.0 41.0 30-31 37.80887051163694 40.0 37.0 41.0 33.0 41.0 32-33 37.69415247072543 40.0 37.0 41.0 33.0 41.0 34-35 37.53940799766775 40.0 36.5 41.0 32.0 41.0 36-37 37.30180302220495 39.5 36.0 41.0 31.5 41.0 38-39 37.150416014770904 39.0 36.0 41.0 31.0 41.0 40-41 36.950800252660216 39.0 36.0 41.0 31.0 41.0 42-43 36.72620455760167 39.0 35.0 41.0 30.0 41.0 44-45 36.51690335746562 39.0 35.0 40.0 30.0 41.0 46-47 36.211920509207516 39.0 35.0 40.0 30.0 41.0 48-49 35.890826490452355 39.0 35.0 40.0 29.0 41.0 50-51 35.59084028958748 38.0 35.0 40.0 28.0 41.0 52-53 35.312705893785534 38.0 34.0 40.0 27.5 41.0 54-55 35.042910645741216 38.0 34.0 40.0 27.0 41.0 56-57 34.722173655313156 38.0 34.0 40.0 26.0 41.0 58-59 34.37793032408533 37.5 33.0 40.0 26.0 41.0 60-61 34.014384723774356 37.0 33.0 40.0 25.5 41.0 62-63 33.60833914775764 36.0 32.5 39.0 24.5 40.5 64-65 33.1873411398863 36.0 32.0 39.0 23.0 40.0 66-67 32.71736961274962 35.0 31.5 38.5 22.0 40.0 68-69 32.42685253389048 35.0 31.0 38.0 22.0 40.0 70-71 31.997625771342502 35.0 31.0 37.5 21.5 39.5 72-73 31.54258063262232 35.0 31.0 37.0 20.0 39.0 74-75 31.00759030173461 34.0 30.0 36.0 18.5 39.0 76-77 29.58756736796074 32.5 28.5 35.0 17.5 37.0 78-79 30.37568514649434 34.0 30.0 35.0 18.0 37.0 80-81 30.17780418832904 34.0 30.0 35.0 16.0 37.0 82-83 29.843069432972158 34.0 29.5 35.0 12.0 36.5 84-85 29.425104708226034 34.0 29.0 35.0 7.5 36.0 86-87 29.04450415431709 34.0 29.0 35.0 6.0 36.0 88-89 28.642636800932898 33.0 29.0 35.0 2.0 35.0 90-91 28.159958408240612 33.0 27.5 35.0 2.0 35.0 92-93 27.652916379184685 33.0 27.0 35.0 2.0 35.0 94-95 27.043656965162043 32.5 25.5 34.0 2.0 35.0 96-97 26.266086779068072 32.0 25.0 34.0 2.0 35.0 98-99 25.287271561148632 32.0 22.0 34.0 2.0 35.0 100-101 22.015920509207522 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22174619456095712 1101 2 0.34492869356790123 1101 3 0.25880846733103624 1101 4 0.14390294204517318 1101 5 0.09715255365092901 1101 6 0.12748273386255704 1101 7 0.12785372332439238 1101 8 0.05200259365908977 1101 9 0.26202120191471323 1101 10-11 0.244688214916998 1101 12-13 0.2328054391661354 1101 14-15 0.37639110591388203 1101 16-17 0.34919378785538413 1101 18-19 0.3051256843055228 1101 20-21 0.21792794376374047 1101 22-23 0.2132255352632697 1101 24-25 0.17244814980861634 1101 26-27 0.11206865833070623 1101 28-29 0.02259433234493713 1101 30-31 0.2836038529487084 1101 32-33 0.1947305994390902 1101 34-35 0.2025806455621293 1101 36-37 0.26987788964971315 1101 38-39 0.3094648373047306 1101 40-41 0.16799731258066686 1101 42-43 0.29080428685437454 1101 44-45 0.2725587093055566 1101 46-47 0.2484622926509772 1101 48-49 0.2921690219993991 1101 50-51 0.1516710886333854 1101 52-53 0.24675237343711132 1101 54-55 0.2521300886951252 1101 56-57 0.1359820509975691 1101 58-59 0.17893110436533277 1101 60-61 0.24690857370455177 1101 62-63 0.13271588092714381 1101 64-65 0.06586298989555672 1101 66-67 0.08399034635467473 1101 68-69 -0.16148492034074025 1101 70-71 0.011614312327594689 1101 72-73 0.036514047303743524 1101 74-75 0.13503159621640037 1101 76-77 0.1510799174866122 1101 78-79 0.048113594482341426 1101 80-81 0.0931143826375802 1101 82-83 0.2834956897544316 1101 84-85 0.045073553273368816 1101 86-87 0.08795650915445563 1101 88-89 -0.00367701321815872 1101 90-91 -0.08321110427297995 1101 92-93 -0.22495611652485437 1101 94-95 0.06222586411024267 1101 96-97 0.42186626609056077 1101 98-99 0.6195152283672556 1101 100-101 0.8753804644863337 1102 1 0.2410537142833391 1102 2 0.17916557490011087 1102 3 0.2553002207006827 1102 4 0.16544452213390315 1102 5 0.07115706224006146 1102 6 0.2101472870783354 1102 7 0.24551628210730314 1102 8 0.18663800917124718 1102 9 0.26206746775550727 1102 10-11 0.2853254808354322 1102 12-13 0.18984510513358543 1102 14-15 0.3798653636341669 1102 16-17 0.3592894035474359 1102 18-19 0.35010619865663273 1102 20-21 0.19693592683794492 1102 22-23 0.15020384385915264 1102 24-25 0.21637608786286222 1102 26-27 -0.06548849845491134 1102 28-29 0.038950898519239274 1102 30-31 0.1625476890598705 1102 32-33 0.17042688044985255 1102 34-35 0.20352403674822028 1102 36-37 0.3347568078520453 1102 38-39 0.4146206139359805 1102 40-41 0.44405597903089244 1102 42-43 0.4584159361611242 1102 44-45 0.387091162292009 1102 46-47 0.37716402882497135 1102 48-49 0.3392308171344851 1102 50-51 0.21006210364795663 1102 52-53 0.2893769675168798 1102 54-55 0.49041644742226254 1102 56-57 0.3036356464842953 1102 58-59 0.20341784056262924 1102 60-61 0.2927596133843835 1102 62-63 0.29581490562730295 1102 64-65 0.42911275939049887 1102 66-67 0.26487722273632386 1102 68-69 0.25583137215804896 1102 70-71 0.20016477852698955 1102 72-73 0.2076877684880536 1102 74-75 0.19790869265862554 1102 76-77 -0.09732717917045974 1102 78-79 0.047288709780335836 1102 80-81 0.3845849736478897 1102 82-83 0.21684912087806651 1102 84-85 0.10802425446230757 1102 86-87 0.12582436095416938 1102 88-89 0.10834725145337032 1102 90-91 0.25088629748514535 1102 92-93 0.27012935990019926 1102 94-95 0.30014880295282964 1102 96-97 0.4780565275971398 1102 98-99 0.6676933147961677 1102 100-101 1.030361937120091 1103 1 0.0362398604706442 1103 2 0.01746359626960725 1103 3 0.057662637834283714 1103 4 0.054791670549903415 1103 5 -0.11186405603908156 1103 6 0.06287109861647622 1103 7 0.04927396444944776 1103 8 0.10367483714549053 1103 9 6.616525061957645E-4 1103 10-11 -0.08320090233522848 1103 12-13 0.04137079233117191 1103 14-15 -0.0175609551810183 1103 16-17 0.14750351558319608 1103 18-19 -0.03700155539443273 1103 20-21 0.05735075013735269 1103 22-23 -0.08620754226489424 1103 24-25 -0.13010668442873907 1103 26-27 -0.029098754907423086 1103 28-29 0.012450816744411952 1103 30-31 -0.09463152898502614 1103 32-33 -0.08259091991128997 1103 34-35 -0.05576873741235033 1103 36-37 -0.09380180227106649 1103 38-39 -0.13324518881304925 1103 40-41 -0.15934638050208605 1103 42-43 -0.12493310549391623 1103 44-45 -0.17370061494807487 1103 46-47 -0.3073488936015565 1103 48-49 -0.3700558167604413 1103 50-51 -0.27752895168459446 1103 52-53 -0.34418330957677057 1103 54-55 -0.49028514974494897 1103 56-57 -0.53620711235709 1103 58-59 -0.40146596535370094 1103 60-61 -0.41625863424903287 1103 62-63 -0.566665690732691 1103 64-65 -0.5316798418926965 1103 66-67 -0.20858538464822374 1103 68-69 -0.2764722620895057 1103 70-71 -0.344083932789772 1103 72-73 -0.43975769216176985 1103 74-75 -0.3019483587526217 1103 76-77 -0.47690541101887973 1103 78-79 -0.540557146547517 1103 80-81 -0.4574615282490697 1103 82-83 -0.3789816559187571 1103 84-85 -0.5694141434513966 1103 86-87 -0.6720315132199417 1103 88-89 -0.6166934270911888 1103 90-91 -0.5242471030763731 1103 92-93 -0.2842179996768479 1103 94-95 -0.2619427306108477 1103 96-97 -0.2545587093947965 1103 98-99 -0.021616854685461817 1103 100-101 0.2425577293042025 1104 1 0.1568056444612509 1104 2 0.22532410125123903 1104 3 0.09017769636366069 1104 4 0.1145014623823073 1104 5 0.01853558691276902 1104 6 0.1253484871454731 1104 7 0.19081147344667926 1104 8 0.07843475304503045 1104 9 0.14352412267326287 1104 10-11 0.16278698217816867 1104 12-13 0.0573908419580178 1104 14-15 0.05032892103597675 1104 16-17 0.22585693879416624 1104 18-19 0.11072427519238204 1104 20-21 0.08983727796248075 1104 22-23 0.11154468810219242 1104 24-25 0.06902431293838873 1104 26-27 0.15611008747997346 1104 28-29 0.12923811738463797 1104 30-31 0.031162891308945007 1104 32-33 0.06184807883857246 1104 34-35 0.005142658769038633 1104 36-37 0.1524630431659162 1104 38-39 0.08132766747743858 1104 40-41 0.2701721253887328 1104 42-43 -0.02637656971333513 1104 44-45 -0.018580833344103098 1104 46-47 -0.07845918785848482 1104 48-49 -0.16096115164698688 1104 50-51 -0.21777494317984036 1104 52-53 -0.1819394347993395 1104 54-55 -0.29784880517928514 1104 56-57 -0.16438686526461765 1104 58-59 -0.23274834355955676 1104 60-61 -0.14093982434639685 1104 62-63 -0.25037246849152694 1104 64-65 0.08215121608737519 1104 66-67 -0.16915370207065905 1104 68-69 0.08630458044941491 1104 70-71 -0.10353665232229758 1104 72-73 -0.06302702674524596 1104 74-75 -0.10321217424301565 1104 76-77 -0.10261471273877376 1104 78-79 -0.015162511266993306 1104 80-81 -0.02100467472404688 1104 82-83 0.05246509939434674 1104 84-85 -0.06443766648432003 1104 86-87 -0.09903612075110146 1104 88-89 0.07070091459536698 1104 90-91 0.2190236758000168 1104 92-93 0.12066015882601278 1104 94-95 0.359883613291867 1104 96-97 0.5520541987955276 1104 98-99 0.5981324302711606 1104 100-101 1.133522078946207 1105 1 0.09166898373635135 1105 2 0.15805954391314714 1105 3 0.19160372131909753 1105 4 0.10208873213869651 1105 5 0.06851144846951485 1105 6 0.0638307361056647 1105 7 0.11726163684537028 1105 8 0.09824313094163983 1105 9 0.023375949767739712 1105 10-11 -0.01640660813674799 1105 12-13 -0.13465832619409213 1105 14-15 -0.0152004535926622 1105 16-17 0.08326651247212169 1105 18-19 0.10562549325535997 1105 20-21 0.13867677588286398 1105 22-23 0.09955159541056702 1105 24-25 0.09786325381777772 1105 26-27 0.058833089559591656 1105 28-29 0.1152656723460126 1105 30-31 0.16028963670407848 1105 32-33 0.1727549415836691 1105 34-35 0.16665669620218893 1105 36-37 0.15270257079514948 1105 38-39 0.27286342950952047 1105 40-41 0.26221015272087556 1105 42-43 0.09617059309118048 1105 44-45 0.04122123142118994 1105 46-47 0.19778951237918818 1105 48-49 0.16822593985628487 1105 50-51 0.20620102978510602 1105 52-53 0.18911021612164092 1105 54-55 0.22670092001382613 1105 56-57 0.12299522772409688 1105 58-59 0.20329685103342854 1105 60-61 0.04013006573084965 1105 62-63 0.06832461261364386 1105 64-65 0.10483299713312277 1105 66-67 0.28059562948014616 1105 68-69 0.3120593710020003 1105 70-71 0.34931632599024454 1105 72-73 0.20328870409254307 1105 74-75 -0.00257993271003798 1105 76-77 0.009967915009298167 1105 78-79 0.1555547718583803 1105 80-81 0.29033018118801124 1105 82-83 0.16815137390415202 1105 84-85 0.16633420665887755 1105 86-87 0.18284704918353967 1105 88-89 0.3379162666452764 1105 90-91 0.519094234326456 1105 92-93 0.5497562669662379 1105 94-95 0.41754849742734024 1105 96-97 0.5051949816238093 1105 98-99 0.6448774540382445 1105 100-101 0.614485202832121 1106 1 0.15985125764199637 1106 2 0.19485833142736197 1106 3 0.15779396127722123 1106 4 0.043042418879345234 1106 5 0.05250629748821467 1106 6 0.07600767674047404 1106 7 0.11786462457077818 1106 8 0.08979036541415297 1106 9 0.16848531697567637 1106 10-11 0.1252992833306692 1106 12-13 0.03470954579699281 1106 14-15 0.2611299670143836 1106 16-17 0.3206624284053987 1106 18-19 0.11267267629618516 1106 20-21 0.14681444093626794 1106 22-23 0.09135735549931923 1106 24-25 0.07160692088417875 1106 26-27 0.14001853139686204 1106 28-29 0.18240166945021485 1106 30-31 0.2807123140424963 1106 32-33 0.145195108300463 1106 34-35 0.2309530130422317 1106 36-37 0.14378958930664254 1106 38-39 0.3816301236498276 1106 40-41 0.4254152063074912 1106 42-43 0.46539484697361644 1106 44-45 0.3720583700484781 1106 46-47 0.3600536915750041 1106 48-49 0.2785519256194675 1106 50-51 0.24500747606372641 1106 52-53 0.18464340648790056 1106 54-55 0.17674043777989823 1106 56-57 0.11202979632940924 1106 58-59 0.09816064820247306 1106 60-61 0.10209620154436294 1106 62-63 0.14793644007920648 1106 64-65 0.15780926433832576 1106 66-67 0.187591641245632 1106 68-69 0.25318011307663824 1106 70-71 0.21780432547983253 1106 72-73 0.047889548613898825 1106 74-75 0.12736405232138637 1106 76-77 -0.2093457258464717 1106 78-79 -0.04899790916489266 1106 80-81 0.11008997566958811 1106 82-83 0.35763919240627473 1106 84-85 0.12614789373806445 1106 86-87 0.13667551994364757 1106 88-89 0.28800330989607303 1106 90-91 0.4659562502663732 1106 92-93 0.34479831725471044 1106 94-95 0.5170908204990745 1106 96-97 0.6023317990308996 1106 98-99 0.8715753833593851 1106 100-101 1.2237213576167676 1107 1 0.012507713591734415 1107 2 0.1069030092765928 1107 3 0.05095989944941692 1107 4 0.03636225953575689 1107 5 0.02328120645589138 1107 6 0.028882379622451992 1107 7 -0.006641358152407406 1107 8 -0.04638135746807137 1107 9 0.03377910114473792 1107 10-11 0.00591897147370446 1107 12-13 0.03335588800513989 1107 14-15 0.16699764337780465 1107 16-17 -0.039388135060633545 1107 18-19 0.05370433140575415 1107 20-21 0.11808038584431557 1107 22-23 0.10274153955536747 1107 24-25 0.20469980779546404 1107 26-27 0.24505560087549583 1107 28-29 0.056866765273646536 1107 30-31 0.06619290416626455 1107 32-33 0.1365004828572225 1107 34-35 0.11127043953688087 1107 36-37 0.16834145184908067 1107 38-39 0.23571578642253854 1107 40-41 0.19111459638502737 1107 42-43 -0.03385287338515042 1107 44-45 0.038851366751572414 1107 46-47 0.13570235830824373 1107 48-49 0.023839186231256804 1107 50-51 0.11799586025276909 1107 52-53 0.07291700432278247 1107 54-55 0.11026344963473633 1107 56-57 -0.0284082517614479 1107 58-59 0.20796654550873228 1107 60-61 0.15897308977871205 1107 62-63 0.16595052666455246 1107 64-65 0.3366656656149445 1107 66-67 0.2961416423857415 1107 68-69 0.29761434453266133 1107 70-71 0.13880035132559243 1107 72-73 0.14137174433476574 1107 74-75 0.16375555539458375 1107 76-77 0.10330021221039587 1107 78-79 0.24293180310927553 1107 80-81 0.5049676596259118 1107 82-83 0.3158666063911184 1107 84-85 0.3394484939629976 1107 86-87 0.44959180952039546 1107 88-89 0.5422288792527006 1107 90-91 0.6741673488056357 1107 92-93 0.6444140459208576 1107 94-95 0.7359690097755411 1107 96-97 0.833573492241289 1107 98-99 0.8444505291827724 1107 100-101 1.1148230970660507 1108 1 0.013181072752974643 1108 2 0.002927503111244789 1108 3 -0.02274447691093684 1108 4 -0.014948182747403393 1108 5 -0.0745704458821379 1108 6 0.04022397662173205 1108 7 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-1.5196368727236695 2316 100-101 -1.5303840338720534 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 8.0 4 43.0 5 87.0 6 167.0 7 405.0 8 944.0 9 1725.0 10 2946.0 11 4350.0 12 6043.0 13 8413.0 14 10812.0 15 14052.0 16 17813.0 17 22465.0 18 20421.0 19 15455.0 20 14641.0 21 15296.0 22 16783.0 23 19122.0 24 22308.0 25 27193.0 26 33402.0 27 41563.0 28 51616.0 29 63798.0 30 78178.0 31 97411.0 32 121695.0 33 154228.0 34 202189.0 35 274669.0 36 386981.0 37 506745.0 38 302327.0 39 16327.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7470084532849 19.157525694463075 14.887253889798158 23.208211962453866 2 15.983723017632489 20.223505296666584 39.183670772064325 24.6091009136366 3 19.935409838104775 23.536303589309814 29.364889322876174 27.163397249709238 4 13.568194504262593 16.6977375523398 36.131449281897154 33.60261866150045 5 14.341472174864203 36.78089343007585 33.83271100621083 15.04492338884911 6 33.0444709889253 35.725627676987784 16.752059475530988 14.477841858555923 7 29.637238229434914 31.9374957485059 19.87705164957971 18.54821437247947 8 26.787036587143483 34.92226811136485 18.937855303435207 19.35283999805646 9 26.146453413024872 16.95523492703451 20.010915086998065 36.887396572942556 10-11 25.607737652155492 26.239585013692295 26.736977744435137 21.41569958971708 12-13 26.349517318049216 23.363516746643967 27.41631536905199 22.870650566254827 14-15 22.972452671401875 25.215577014836317 25.574595079438968 26.23737523432284 16-17 22.726917579540633 28.39337032123342 26.275377028189506 22.604335071036438 18-19 22.529886788785774 26.93031436762062 28.520693843836547 22.019104999757058 20-21 23.169447548709975 25.918740585977357 28.738817355813612 22.172994509499052 22-23 22.88687685186715 25.799739365945957 28.663332131553144 22.650051650633753 24-25 22.993520570410592 26.11833228834764 28.60707617926279 22.281070961978976 26-27 22.766639164317304 26.29232373472186 28.64390094100795 22.29713615995289 28-29 22.46222304806124 26.594616531673086 28.633825571396805 22.30933484886887 30-31 22.704467667860662 26.127995273255216 28.52503017381282 22.6425068850713 32-33 22.63262631561251 26.280939597028468 28.570887154249945 22.515546933109075 34-35 23.135316899184126 26.28558100208464 28.28143116074075 22.297670937990485 36-37 23.128416800868482 26.063660740405258 28.34915732275399 22.458765135972275 38-39 22.809170293963636 26.33520616795334 28.49914457083346 22.35647896724956 40-41 23.016470280921087 26.278399096935246 28.296786913335403 22.40834370880826 42-43 22.847890439648925 26.36503907802564 27.921221623413896 22.865848858911544 44-45 23.03977846411713 26.36293332136094 27.88144456228957 22.715843652232355 46-47 23.228691167327373 26.7376952991002 27.43412417941289 22.599489354159534 48-49 23.321815192634247 26.687970594826222 27.391633822246952 22.59858039029258 50-51 23.050949642754272 26.82005629326607 27.35230014471746 22.776693919262193 52-53 23.430294120390958 26.9768307890375 26.968220661817526 22.624654428754017 54-55 23.10288215093212 26.7219894108567 27.157725991097127 23.017402447114048 56-57 23.14533086706874 27.04610135772181 27.043224869649418 22.765342905560036 58-59 23.464782496560375 26.717378176830643 27.068308357545302 22.749530969063684 60-61 23.293187131343114 26.79625844601817 27.465778611894827 22.444775810743884 62-63 23.06236370209408 26.907840263713368 27.360475648107474 22.669320386085076 64-65 22.93484130072489 26.948535228646147 27.18061593519778 22.936007535431184 66-67 23.021533094774266 27.06885463076502 26.922481130918474 22.98713114354224 68-69 22.890823946670462 26.824456673636448 27.260272268616188 23.024447111076903 70-71 23.11538578151007 26.777855433095105 27.154358471629337 22.95240031376549 72-73 23.166545679293087 26.732845302337243 27.16600925334756 22.934599765022114 74-75 22.983749802232182 27.234251559110128 26.803010813380652 22.978987825277038 76-77 23.055962144975524 27.036776523229953 26.849977541751834 23.05728379004269 78-79 23.04822634411204 27.023872066574267 26.878139789090525 23.049761800223166 80-81 23.175647926303466 27.22018366990341 26.77777278910692 22.826395614686206 82-83 23.195177278663333 27.207302498222052 26.718651329772193 22.87886889334242 84-85 23.059165521690346 27.22852496404719 26.606826630986298 23.105482883276167 86-87 23.03732846546219 27.112427765213326 26.757988489081107 23.092255280243375 88-89 23.008055556203463 27.210457528535763 26.514819315608158 23.266667599652617 90-91 23.079254123204453 27.1156783137591 26.63620497052931 23.16886259250714 92-93 23.201194749492682 26.844741456030974 26.81218670915045 23.141877085325895 94-95 22.977577181933313 26.880598999128498 26.865128016550067 23.27669580238813 96-97 22.950646662976485 26.85575481848657 26.946655357709265 23.24694316082768 98-99 22.775166859084248 27.01321267390468 26.50202330001982 23.70959716699125 100-101 22.560864561615197 27.21700668359058 26.530513476316468 23.691615278477755 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 246.0 1 175.5 2 163.0 3 208.5 4 175.0 5 185.0 6 323.5 7 634.5 8 1051.0 9 1364.5 10 1427.0 11 1394.0 12 1533.5 13 1704.0 14 1931.0 15 2246.0 16 2503.0 17 2728.0 18 2975.5 19 3264.0 20 3562.5 21 4019.0 22 4723.0 23 5703.0 24 7007.5 25 8660.0 26 10815.5 27 13750.5 28 16833.5 29 20525.5 30 25391.0 31 30437.5 32 35994.0 33 40888.0 34 45903.0 35 50990.5 36 57322.0 37 63673.5 38 67230.0 39 69704.5 40 71739.5 41 75788.0 42 80397.0 43 84589.0 44 88444.5 45 91716.5 46 93651.0 47 97862.0 48 108184.0 49 108606.0 50 100514.0 51 96232.5 52 93361.5 53 92240.5 54 91008.5 55 86036.5 56 78447.5 57 71419.0 58 65248.5 59 57580.5 60 47955.0 61 39256.5 62 32171.5 63 25535.0 64 19680.0 65 15364.5 66 11908.0 67 9761.5 68 7532.0 69 5182.0 70 3815.0 71 2957.5 72 2429.0 73 1918.5 74 1488.5 75 1194.5 76 847.0 77 500.0 78 303.0 79 192.0 80 126.5 81 79.0 82 46.0 83 26.5 84 17.0 85 12.0 86 4.0 87 3.0 88 5.5 89 2.5 90 0.0 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03739371264758758 2 0.0061415869005393324 3 0.0037704679072931347 4 5.05320441183616E-4 5 8.940284728633206E-4 6 1.5548321267188183E-4 7 0.0 8 0.0 9 0.001515961323550848 10-11 3.887080316797046E-4 12-13 7.774160633594091E-4 14-15 0.002040717166318449 16-17 0.0027986978280938727 18-19 0.0 20-21 0.0 22-23 0.002040717166318449 24-25 8.5515766969535E-4 26-27 0.004567319372236528 28-29 0.006102716097371362 30-31 0.0011661240950391137 32-33 4.470142364316603E-4 34-35 0.009231815752392984 36-37 0.012341480005830619 38-39 0.009018026334969146 40-41 0.002021281764734464 42-43 6.024974491035421E-4 44-45 0.0015353967251348331 46-47 1.943540158398523E-5 48-49 6.413682522715125E-4 50-51 0.001010640882367232 52-53 0.0028375686312618435 54-55 0.0032845828676935037 56-57 0.0016908799378067148 58-59 0.0013021719061270104 60-61 0.004314659151644721 62-63 0.005247558427676012 64-65 0.009484475972984793 66-67 0.003751032505709149 68-69 0.0035761138914532824 70-71 1.1661240950391136E-4 72-73 0.0016520091346387447 74-75 0.006374811719547155 76-77 0.0028570040328458287 78-79 0.003867644915213061 80-81 0.005033769010252174 82-83 0.005266993829259997 84-85 0.006044409892619407 86-87 0.004314659151644721 88-89 0.00929012195714494 90-91 0.012574704824838443 92-93 0.0013799135124629514 94-95 0.002604343812254021 96-97 0.001282736504543025 98-99 0.0036927263009571935 100-101 0.005072639813420145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2572625.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.901720609518094 #Duplication Level Percentage of deduplicated Percentage of total 1 77.85754310573932 44.302281651462906 2 12.879967812637663 14.657846598685886 3 3.925965711713206 6.701826121513582 4 1.797431912455904 4.091078739887905 5 0.9260774297878324 2.6347699586283926 6 0.5773468581308054 1.97112177696853 7 0.3724096172292089 1.4833523594311817 8 0.2604646807342682 1.185671079343093 9 0.1942909089855571 0.994993831805893 >10 1.0701784688057363 11.209496462957217 >50 0.08934291251065628 3.5465609071611426 >100 0.045488560636233404 4.782693420403137 >500 0.0023964847485476023 0.9257564323758704 >1k 8.901229065941393E-4 0.8027560091125272 >5k 2.0541297844480134E-4 0.7097946502626684 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6952 0.2702298236237306 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6115 0.23769496137213933 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5151 0.20022350711821585 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3629 0.1410621446965648 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.887080316797046E-5 0.0 0.0 0.0 6 0.0 3.887080316797046E-5 0.0 0.0 0.0 7 0.0 3.887080316797046E-5 0.0 0.0 0.0 8 0.0 3.887080316797046E-5 0.0 0.0 0.0 9 0.0 3.887080316797046E-5 0.0 0.0 0.0 10-11 0.0 3.887080316797046E-5 0.0 0.0 0.0 12-13 0.0 3.887080316797046E-5 0.0 0.0 0.0 14-15 0.0 3.887080316797046E-5 0.0 0.0 0.0 16-17 0.0 3.887080316797046E-5 0.0 3.887080316797046E-5 0.0 18-19 0.0 3.887080316797046E-5 0.0 7.774160633594091E-5 3.887080316797046E-5 20-21 0.0 5.8306204751955686E-5 0.0 1.1661240950391137E-4 3.887080316797046E-5 22-23 0.0 7.774160633594091E-5 0.0 1.7491861425586707E-4 3.887080316797046E-5 24-25 0.0 7.774160633594091E-5 0.0 3.304018269277489E-4 7.774160633594091E-5 26-27 0.0 7.774160633594091E-5 0.0 5.247558427676012E-4 7.774160633594091E-5 28-29 0.0 7.774160633594091E-5 0.0 0.001010640882367232 7.774160633594091E-5 30-31 0.0 7.774160633594091E-5 0.0 0.0020018463631504787 7.774160633594091E-5 32-33 0.0 7.774160633594091E-5 0.0 0.0037121617025411787 7.774160633594091E-5 34-35 0.0 7.774160633594091E-5 0.0 0.00550021864826782 7.774160633594091E-5 36-37 0.0 7.774160633594091E-5 0.0 0.007735289830426121 7.774160633594091E-5 38-39 0.0 7.774160633594091E-5 0.0 0.011777853359895049 7.774160633594091E-5 40-41 0.0 7.774160633594091E-5 0.0 0.016461785141635488 7.774160633594091E-5 42-43 0.0 7.774160633594091E-5 0.0 0.02097079830912006 7.774160633594091E-5 44-45 0.0 7.774160633594091E-5 0.0 0.0232836110976143 7.774160633594091E-5 46-47 0.0 7.774160633594091E-5 0.0 0.026743112579563674 7.774160633594091E-5 48-49 0.0 7.774160633594091E-5 0.0 0.031154948739128323 7.774160633594091E-5 50-51 0.0 7.774160633594091E-5 0.0 0.036927263009571935 7.774160633594091E-5 52-53 0.0 7.774160633594091E-5 0.0 0.04602303095087702 7.774160633594091E-5 54-55 0.0 7.774160633594091E-5 0.0 0.052397842670424175 7.774160633594091E-5 56-57 0.0 7.774160633594091E-5 0.0 0.07084203877362616 7.774160633594091E-5 58-59 0.0 7.774160633594091E-5 0.0 0.09877071084981293 7.774160633594091E-5 60-61 0.0 7.774160633594091E-5 0.0 0.12370633108206598 9.717700791992614E-5 62-63 0.0 7.774160633594091E-5 0.0 0.1386715903017346 1.1661240950391137E-4 64-65 0.0 1.1661240950391137E-4 0.0 0.16823283611097614 1.1661240950391137E-4 66-67 0.0 1.1661240950391137E-4 0.0 0.224984208736213 1.360478110878966E-4 68-69 0.0 1.1661240950391137E-4 0.0 0.30367814974976926 1.5548321267188183E-4 70-71 0.0 1.1661240950391137E-4 0.0 0.36905884067829553 1.5548321267188183E-4 72-73 0.0 1.1661240950391137E-4 0.0 0.44433215101307033 1.5548321267188183E-4 74-75 0.0 1.1661240950391137E-4 0.0 0.5363393421116563 1.5548321267188183E-4 76-77 0.0 1.1661240950391137E-4 0.0 0.5976774695107138 1.5548321267188183E-4 78-79 0.0 1.1661240950391137E-4 0.0 0.6779262426509888 1.5548321267188183E-4 80-81 0.0 1.5548321267188183E-4 0.0 0.7840629707011322 1.5548321267188183E-4 82-83 1.943540158398523E-5 1.5548321267188183E-4 0.0 0.8773140275010932 1.5548321267188183E-4 84-85 3.887080316797046E-5 1.5548321267188183E-4 0.0 1.0168990816772752 1.5548321267188183E-4 86-87 3.887080316797046E-5 1.5548321267188183E-4 0.0 1.2300860016520092 1.5548321267188183E-4 88-89 3.887080316797046E-5 1.5548321267188183E-4 0.0 1.4990719595743647 1.5548321267188183E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8720 0.0 23.268074 1 GTATCAA 14520 0.0 22.711296 1 TACATGG 8830 0.0 22.217916 2 GGTATCA 11060 0.0 21.610355 1 ACATGGG 8710 0.0 21.377066 3 GAGTACT 8635 0.0 20.930157 12-13 TACCTGG 2170 0.0 20.795904 2 GTACCTG 2330 0.0 20.393564 1 GTGTAGC 1370 0.0 20.116688 1 TAAGGTG 1230 0.0 20.08029 5 GTACTTT 9050 0.0 19.760056 14-15 TCAACGC 16850 0.0 19.506435 4 ATCAACG 16860 0.0 19.4389 3 AGTACTT 9085 0.0 19.422901 12-13 AGAGTAC 12955 0.0 19.396141 10-11 CAACGCA 16935 0.0 19.380484 5 TATCAAC 17160 0.0 19.211273 2 AACGCAG 17170 0.0 19.1713 6 CATGGGG 5595 0.0 18.167093 4 GTAAGGT 970 0.0 18.117643 4 >>END_MODULE