FastQCFastQC Report
Thu 26 May 2016
SRR936624_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936624_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647942
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57490.21711200622974372No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47140.17802504737641534No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41180.15551700150532No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28690.10834829463787349No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27730.10472283758481114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA127950.024.402061
GGTATCA94150.023.622561
GTACATG93000.023.5569691
TACATGG94550.022.6170522
ACATGGG93800.022.1397043
GAGTACT67850.021.57462912-13
TCAACGC146500.021.149254
ATCAACG147300.021.0025223
CAACGCA148200.020.8433065
TATCAAC149700.020.6654192
AACGCAG151250.020.3911886
GTACTTT72900.019.81575214-15
AGAGTAC118850.019.17647710-11
GTATAGG8000.019.0090941
GTACCTG22300.018.9664731
GTGTAGC13900.018.803961
AGTACTT73700.018.73359312-13
ACGCAGA167850.018.2329837
CGCAGAG169550.018.1940928
CATGGGG58200.017.6366374
Produced by FastQC (version 0.11.5)