##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936624_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2647942 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.113092733904292 31.0 31.0 34.0 28.0 34.0 2 31.17636753372997 31.0 31.0 34.0 27.0 34.0 3 31.126932916204357 31.0 31.0 34.0 27.0 34.0 4 34.77900422290217 37.0 35.0 37.0 32.0 37.0 5 34.803696984299506 37.0 35.0 37.0 32.0 37.0 6 34.84974784190892 37.0 35.0 37.0 32.0 37.0 7 34.70054895462212 37.0 35.0 37.0 31.0 37.0 8 34.67654049824354 37.0 35.0 37.0 31.0 37.0 9 36.137388960936455 39.0 35.0 39.0 31.0 39.0 10-11 35.999393680073055 39.0 35.0 39.0 30.0 39.0 12-13 35.888729247090765 38.5 35.0 39.0 30.0 39.0 14-15 36.84542335141782 39.0 36.0 41.0 30.0 41.0 16-17 36.74241524172358 39.0 36.0 41.0 30.0 41.0 18-19 36.59242158627342 39.0 36.0 41.0 30.0 41.0 20-21 36.56018447533972 39.0 36.0 41.0 30.0 41.0 22-23 36.5560342711434 39.0 36.0 40.5 30.0 41.0 24-25 36.68309653308116 39.0 36.0 41.0 30.0 41.0 26-27 36.609355680751314 39.0 36.0 41.0 30.0 41.0 28-29 36.488526561382386 39.0 36.0 41.0 30.0 41.0 30-31 36.3025644066222 39.0 35.5 40.5 29.5 41.0 32-33 36.15561594627073 39.0 35.0 40.0 29.0 41.0 34-35 35.96114605229269 38.5 35.0 40.0 28.5 41.0 36-37 35.767018877301695 38.0 35.0 40.0 27.5 41.0 38-39 35.58019397705841 38.0 35.0 40.0 27.0 41.0 40-41 35.35642680239975 38.0 34.0 40.0 26.5 41.0 42-43 35.09464803232095 38.0 34.0 40.0 25.5 41.0 44-45 34.96069362546461 38.0 34.0 40.0 25.0 41.0 46-47 34.856041408761975 38.0 34.0 40.0 24.0 41.0 48-49 34.65216760790078 38.0 33.5 40.0 24.0 41.0 50-51 33.67413995472711 37.0 32.0 39.5 23.5 40.5 52-53 33.78917891706087 37.5 33.0 39.5 23.0 40.5 54-55 34.232070037787835 38.0 33.0 40.0 23.0 41.0 56-57 34.07947398394678 38.0 33.0 40.0 23.0 41.0 58-59 33.841650987823755 37.5 33.0 40.0 22.5 41.0 60-61 33.53752102576265 37.0 33.0 40.0 22.0 41.0 62-63 33.18592061306479 36.5 32.0 40.0 20.5 41.0 64-65 32.81954589639803 36.0 32.0 39.0 19.5 41.0 66-67 32.45102460703444 36.0 31.0 39.0 18.5 40.5 68-69 32.005280515962966 35.0 31.0 38.5 17.5 40.0 70-71 31.602856293680148 35.0 31.0 38.0 14.0 40.0 72-73 31.182820847284418 35.0 30.5 37.0 12.0 39.5 74-75 30.75312582375294 35.0 30.0 37.0 8.0 39.0 76-77 30.287654148013814 34.0 29.5 36.0 7.0 39.0 78-79 29.817828147293255 34.0 29.0 36.0 4.5 38.0 80-81 29.363620691087647 34.0 29.0 35.0 2.0 37.0 82-83 28.96455114953424 34.0 29.0 35.0 2.0 37.0 84-85 28.579616358666467 34.0 28.0 35.0 2.0 36.0 86-87 28.225709437744484 34.0 27.0 35.0 2.0 36.0 88-89 27.855073487259162 33.5 26.5 35.0 2.0 35.5 90-91 27.490290006352105 33.0 26.0 35.0 2.0 35.0 92-93 27.048863041562086 33.0 25.5 35.0 2.0 35.0 94-95 26.539433076706363 33.0 25.0 35.0 2.0 35.0 96-97 25.907344458451128 32.5 23.5 35.0 2.0 35.0 98-99 25.126054498172543 32.0 19.0 35.0 2.0 35.0 100-101 22.480913101570955 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6079543758481911 1101 2 0.5492911757445533 1101 3 0.5543131679292763 1101 4 0.6634575740235675 1101 5 0.5214086569758862 1101 6 0.6540342170901425 1101 7 0.6639482158354539 1101 8 0.6250127545378916 1101 9 0.796217510139833 1101 10-11 0.8290133783894476 1101 12-13 0.9634704767486681 1101 14-15 1.07913660258653 1101 16-17 1.1638390689176887 1101 18-19 1.34991663743876 1101 20-21 1.09319903194055 1101 22-23 1.0233901730125012 1101 24-25 0.8915744627172657 1101 26-27 0.9417748000628734 1101 28-29 0.9798979562058179 1101 30-31 0.7939622465677303 1101 32-33 0.7114244778537895 1101 34-35 0.858715822288012 1101 36-37 0.7133400788287005 1101 38-39 0.8580973142856507 1101 40-41 0.7980250282576833 1101 42-43 0.9474630706324447 1101 44-45 0.9196458404478562 1101 46-47 0.721433534669643 1101 48-49 0.9103426366087106 1101 50-51 0.8987541152068701 1101 52-53 0.8870049599925593 1101 54-55 0.918071229249044 1101 56-57 0.9745410940917694 1101 58-59 0.9677201471062631 1101 60-61 0.8560045885839713 1101 62-63 0.9245708960878218 1101 64-65 0.7195370906140255 1101 66-67 0.7521710889248823 1101 68-69 0.9220047891944674 1101 70-71 0.7787759738495339 1101 72-73 0.8887870188357994 1101 74-75 0.6651073282216444 1101 76-77 0.7615175417123083 1101 78-79 0.9126442735110487 1101 80-81 1.040933643560166 1101 82-83 0.9774548359612147 1101 84-85 0.9018771599099118 1101 86-87 0.8728735298925798 1101 88-89 0.9408125694911327 1101 90-91 0.8450271203504904 1101 92-93 0.9250954620304164 1101 94-95 1.113322226106753 1101 96-97 1.1062971984602399 1101 98-99 1.0618290111083724 1101 100-101 1.4647594132765818 1102 1 0.3090532394672145 1102 2 0.42901102520840695 1102 3 0.6179667929765102 1102 4 0.4574244600201709 1102 5 0.44293937857466403 1102 6 0.44170247300877463 1102 7 0.5603785470921707 1102 8 0.35650146208292455 1102 9 0.5196228205510778 1102 10-11 0.543934313702529 1102 12-13 0.5053371088299627 1102 14-15 0.671669896996363 1102 16-17 0.42709310719437354 1102 18-19 0.5801782895846515 1102 20-21 0.6629938241186366 1102 22-23 0.2990654405480484 1102 24-25 0.5370501433027428 1102 26-27 0.35801584369161077 1102 28-29 0.1934776235938216 1102 30-31 0.24761367038730953 1102 32-33 0.4579133963217501 1102 34-35 0.14587696561598307 1102 36-37 0.2342405223053987 1102 38-39 0.1370046392353217 1102 40-41 0.45870634524254683 1102 42-43 0.1566192465008882 1102 44-45 0.46324966973131865 1102 46-47 0.4534036625966422 1102 48-49 0.6845251756002853 1102 50-51 0.5137748798967507 1102 52-53 0.28582180573429383 1102 54-55 0.5314949819540189 1102 56-57 0.3025269887326161 1102 58-59 0.18820271634508146 1102 60-61 0.4829094583009095 1102 62-63 0.35800389798792054 1102 64-65 0.4124484980444336 1102 66-67 0.44390087914182885 1102 68-69 0.45830092409897816 1102 70-71 0.20393888937893223 1102 72-73 0.3541848307122848 1102 74-75 0.2395981288402318 1102 76-77 0.28151460674208906 1102 78-79 0.22621044407500435 1102 80-81 0.4600596753996591 1102 82-83 0.316696161867668 1102 84-85 0.3876124866295392 1102 86-87 0.2524828131046384 1102 88-89 0.21674094454980875 1102 90-91 0.45844083177739847 1102 92-93 0.4691013912087776 1102 94-95 0.5105731373387137 1102 96-97 0.30837494644951846 1102 98-99 0.5065437535445412 1102 100-101 1.1355930233527722 1103 1 0.3771839831124666 1103 2 0.38068376108316215 1103 3 0.4680038892188598 1103 4 0.41111956833402985 1103 5 0.33611026501356633 1103 6 0.32303929972093215 1103 7 0.4795965511351241 1103 8 0.29303731819967993 1103 9 0.5036364906059845 1103 10-11 0.3887147648788556 1103 12-13 0.27695642253155484 1103 14-15 0.5726641479748409 1103 16-17 0.7688691211583247 1103 18-19 0.8391096131499083 1103 20-21 0.844553945120488 1103 22-23 0.5619290554066225 1103 24-25 0.5611380596050211 1103 26-27 0.528136612569476 1103 28-29 0.5227780099682064 1103 30-31 0.5249694609852895 1103 32-33 0.6152664271026751 1103 34-35 0.5895041179148777 1103 36-37 0.6311949566504254 1103 38-39 0.474875691263243 1103 40-41 0.7523069746242044 1103 42-43 0.7712587319562942 1103 44-45 0.663343575195043 1103 46-47 0.7961890706911561 1103 48-49 1.00283333605676 1103 50-51 0.8083898316537201 1103 52-53 0.9792679570439518 1103 54-55 0.9617674870077053 1103 56-57 0.9202797808072845 1103 58-59 0.785389495029257 1103 60-61 0.9180120912380687 1103 62-63 0.8904442931077057 1103 64-65 0.8848960256784508 1103 66-67 0.8298662237247001 1103 68-69 0.8163298412471178 1103 70-71 0.8237369030274806 1103 72-73 0.8699328128200676 1103 74-75 0.8495544377103528 1103 76-77 0.8635277678437205 1103 78-79 0.7800788473953872 1103 80-81 0.9886430469642207 1103 82-83 0.7494601186568168 1103 84-85 0.8430902754784491 1103 86-87 0.8139084557477538 1103 88-89 0.7705416965103566 1103 90-91 0.9897316018022018 1103 92-93 0.8984612749380467 1103 94-95 1.0272055153691362 1103 96-97 0.8957357011340363 1103 98-99 0.8601641317470055 1103 100-101 1.0711723519645489 1104 1 0.30073303395064244 1104 2 0.3306924094824879 1104 3 0.45198903559301584 1104 4 0.4945162632040834 1104 5 0.33959214661697956 1104 6 0.3659209463761641 1104 7 0.3024268839543538 1104 8 0.42650464166423063 1104 9 0.5457893294430676 1104 10-11 0.47295512912060644 1104 12-13 0.40514506934533756 1104 14-15 0.6278526470451382 1104 16-17 0.5348465884745863 1104 18-19 0.5635189094584803 1104 20-21 0.626789051856143 1104 22-23 0.3472541631512627 1104 24-25 0.515101999383333 1104 26-27 0.18533973824512628 1104 28-29 0.05734773408426719 1104 30-31 0.21048528395255772 1104 32-33 0.16475283830522613 1104 34-35 0.3698780170457141 1104 36-37 0.39701837435369924 1104 38-39 0.28160693865071096 1104 40-41 0.37905467198788756 1104 42-43 0.6394350548010337 1104 44-45 0.620978129874544 1104 46-47 0.6883336214788827 1104 48-49 0.7506451108524459 1104 50-51 0.4463975647641192 1104 52-53 0.4898036893471627 1104 54-55 0.6720776402285153 1104 56-57 0.6801776936602479 1104 58-59 0.7117654913969247 1104 60-61 0.5514222091445831 1104 62-63 0.5825243412504619 1104 64-65 0.5252766633537576 1104 66-67 0.5639761412766333 1104 68-69 0.4930229455900559 1104 70-71 0.33403615566163936 1104 72-73 0.6666053411796113 1104 74-75 0.6513304225468275 1104 76-77 0.6608047832811152 1104 78-79 0.5460054152781169 1104 80-81 0.6236233898732557 1104 82-83 0.641440259769869 1104 84-85 0.6082731711066813 1104 86-87 0.634197420390219 1104 88-89 0.7096844419447201 1104 90-91 0.8064044544767199 1104 92-93 0.7053582833125773 1104 94-95 0.7694922242207731 1104 96-97 0.7872271175977055 1104 98-99 0.5420678966835126 1104 100-101 1.2284142152621875 1105 1 0.41751196367928856 1105 2 0.3715142082252392 1105 3 0.4909992034561661 1105 4 0.46617031935233655 1105 5 0.4546242081928753 1105 6 0.43044486786395453 1105 7 0.44248358527464404 1105 8 0.4808467811946642 1105 9 0.4777019625690073 1105 10-11 0.578734115444739 1105 12-13 0.5238940283103801 1105 14-15 0.8838961712785931 1105 16-17 0.743320451940491 1105 18-19 0.643799038735466 1105 20-21 0.5848940668608549 1105 22-23 0.5565835157816679 1105 24-25 0.3894994644574723 1105 26-27 0.42648743269204914 1105 28-29 0.5104726909474593 1105 30-31 0.4376243864676894 1105 32-33 0.516913279161102 1105 34-35 0.30039710122942864 1105 36-37 0.4060431121049177 1105 38-39 0.16890288429695488 1105 40-41 0.3701129869698647 1105 42-43 0.13365604547060883 1105 44-45 0.21344371705507115 1105 46-47 0.5581848696625329 1105 48-49 0.5930352948936175 1105 50-51 0.7320730848674799 1105 52-53 0.8200912381550225 1105 54-55 0.6815827494670188 1105 56-57 0.5556466770303743 1105 58-59 0.7054416929468132 1105 60-61 0.5473303666254452 1105 62-63 0.828817681663601 1105 64-65 0.8125225357939598 1105 66-67 0.8221674759087492 1105 68-69 0.7700012225840354 1105 70-71 0.9293161031888815 1105 72-73 0.7840675579375826 1105 74-75 0.7949322792470426 1105 76-77 0.8095922018733326 1105 78-79 0.8524444637744679 1105 80-81 0.7697353486386085 1105 82-83 0.5956037784081865 1105 84-85 0.7177624980252482 1105 86-87 0.8368245784959321 1105 88-89 0.7111523587429076 1105 90-91 0.7622045260723169 1105 92-93 0.8846031637212413 1105 94-95 1.06848436434937 1105 96-97 1.1936990420928808 1105 98-99 1.3398157987392594 1105 100-101 1.6400768780662496 1106 1 0.10912612007316724 1106 2 -0.03546897538653582 1106 3 -0.08449069643534912 1106 4 0.17909677245486222 1106 5 0.26693689840302426 1106 6 0.053873999002234996 1106 7 0.1495764142189273 1106 8 0.3029504847019382 1106 9 0.21115844466563516 1106 10-11 0.4341774225809516 1106 12-13 0.3060263139479602 1106 14-15 0.20966053964713183 1106 16-17 0.1486004157061558 1106 18-19 0.2638532197717254 1106 20-21 0.12117741445526775 1106 22-23 -0.0967733929160417 1106 24-25 -0.26344849390429204 1106 26-27 -0.35220147809486235 1106 28-29 -0.17340325379943522 1106 30-31 -0.17492674860181268 1106 32-33 -0.12348513044886289 1106 34-35 -0.06464851842651598 1106 36-37 -0.25651739881249824 1106 38-39 -0.009710806619224854 1106 40-41 -0.12231429930460536 1106 42-43 0.04912383872274262 1106 44-45 -0.24618585882040378 1106 46-47 0.089092198402966 1106 48-49 0.11891310865424742 1106 50-51 -0.019546616535443206 1106 52-53 0.0787704676686829 1106 54-55 0.09739592196509506 1106 56-57 0.17693599580873354 1106 58-59 0.06414393590534218 1106 60-61 0.21736213796461357 1106 62-63 0.21115203339711996 1106 64-65 -0.004606818707323157 1106 66-67 -0.05440143965132194 1106 68-69 0.14145574332904687 1106 70-71 -0.14451662742265015 1106 72-73 -0.018812579423325815 1106 74-75 0.1867666615230945 1106 76-77 0.10353533309400831 1106 78-79 0.2598691527496584 1106 80-81 0.2804417501171521 1106 82-83 0.23369616744526311 1106 84-85 0.40941577964304443 1106 86-87 0.5015920243208178 1106 88-89 0.4946888906925295 1106 90-91 0.6755785575306525 1106 92-93 0.6746112207945849 1106 94-95 0.8786206621869077 1106 96-97 0.9128159224546906 1106 98-99 1.0415984883669687 1106 100-101 1.7171925760294648 1107 1 0.27836934494672505 1107 2 0.40025597194791374 1107 3 0.4167927436887062 1107 4 0.4394121279485006 1107 5 0.4210746541510204 1107 6 0.42140455834700674 1107 7 0.42958673314416274 1107 8 0.5215636744587897 1107 9 0.553690122528586 1107 10-11 0.6195255244793785 1107 12-13 0.4662607077634604 1107 14-15 0.3583876421519463 1107 16-17 0.5526692754934786 1107 18-19 0.7453474553573542 1107 20-21 0.4542192984397033 1107 22-23 0.5178302185174033 1107 24-25 0.4716772370592466 1107 26-27 0.4340670690785302 1107 28-29 0.5137558956317392 1107 30-31 0.31138354071838137 1107 32-33 0.3689026393401065 1107 34-35 0.38432622876457856 1107 36-37 0.33353973604842935 1107 38-39 0.41219710934817044 1107 40-41 0.5059903269818093 1107 42-43 0.4220579118328942 1107 44-45 0.4244856185850594 1107 46-47 0.5389829405945221 1107 48-49 0.4691656557819357 1107 50-51 0.28221364551195194 1107 52-53 0.36928260144916436 1107 54-55 0.5464540820550354 1107 56-57 0.5934868046163686 1107 58-59 0.665175995016412 1107 60-61 0.6440326805744476 1107 62-63 0.8174532650998216 1107 64-65 0.6330897906118835 1107 66-67 0.580286355744569 1107 68-69 0.5561228009217913 1107 70-71 0.6591338113696565 1107 72-73 0.6255790751934924 1107 74-75 0.5644415858684262 1107 76-77 0.7185068656162343 1107 78-79 0.8524459283139834 1107 80-81 0.7960882726679088 1107 82-83 0.7828902631923249 1107 84-85 0.8246291262780154 1107 86-87 0.8842610133802786 1107 88-89 0.6349363164348212 1107 90-91 0.6712265698524398 1107 92-93 0.9017483520762575 1107 94-95 0.9574037286782939 1107 96-97 1.1092141511026554 1107 98-99 1.3328884682526798 1107 100-101 1.7251720133814992 1108 1 0.12039159185388826 1108 2 0.11910958953909656 1108 3 0.2883726415873795 1108 4 0.3703362721952459 1108 5 0.2914636483139219 1108 6 0.26326836199724113 1108 7 0.40696717726675047 1108 8 0.3186568179680904 1108 9 -0.03881003345818357 1108 10-11 0.157580032890138 1108 12-13 0.2189579278580993 1108 14-15 0.6081314308078021 1108 16-17 0.8249175337230596 1108 18-19 0.6218623080002175 1108 20-21 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0.5283210375256289 2311 10-11 0.390795656388633 2311 12-13 0.483725844185642 2311 14-15 0.811327982126187 2311 16-17 0.8493531516322861 2311 18-19 0.8592775495648155 2311 20-21 0.6516674349747049 2311 22-23 0.6550060277895966 2311 24-25 0.550425703367381 2311 26-27 0.48939059579414135 2311 28-29 0.6388512372043067 2311 30-31 0.7817615458142342 2311 32-33 0.7041369768792478 2311 34-35 0.707551788415202 2311 36-37 0.6855671180305407 2311 38-39 0.7704698785022757 2311 40-41 0.9205215285030448 2311 42-43 0.869329615041778 2311 44-45 0.8934045597382934 2311 46-47 0.8370812214493384 2311 48-49 0.6388833507077081 2311 50-51 0.8806315630287074 2311 52-53 0.8018484914370347 2311 54-55 0.7986601597056762 2311 56-57 0.6627047863013829 2311 58-59 0.6599877387476667 2311 60-61 0.7320561631009355 2311 62-63 0.6664394125128794 2311 64-65 0.48453616193220483 2311 66-67 0.5564979723145527 2311 68-69 0.6532820333942126 2311 70-71 0.5959388021557501 2311 72-73 0.5363102760095764 2311 74-75 0.3283689476363669 2311 76-77 0.6213889135473352 2311 78-79 0.8216234228843646 2311 80-81 0.6810625583553289 2311 82-83 0.7776505900508575 2311 84-85 0.8339082558461861 2311 86-87 0.8859899424520279 2311 88-89 0.8726827942321336 2311 90-91 0.933021012834363 2311 92-93 0.9488845497897032 2311 94-95 0.8470927946893312 2311 96-97 0.6529581573643526 2311 98-99 0.7879624615396921 2311 100-101 0.5741540474447326 2312 1 0.34754521944690353 2312 2 0.35077465192289736 2312 3 0.4042810606521243 2312 4 0.5412721025093461 2312 5 0.4474686541650712 2312 6 0.3449521138692475 2312 7 0.3598574516479829 2312 8 0.49889799787334965 2312 9 0.5432951480497294 2312 10-11 0.497582439314435 2312 12-13 0.24006452878987972 2312 14-15 0.40738495066140956 2312 16-17 0.39466703961208793 2312 18-19 0.4364915965048368 2312 20-21 0.42910706515844765 2312 22-23 0.5545966467993679 2312 24-25 0.5079615570772305 2312 26-27 0.37482162926929874 2312 28-29 0.2701241753968304 2312 30-31 0.4466827670026987 2312 32-33 0.49821994592875996 2312 34-35 0.3553073520226704 2312 36-37 0.4791153495981675 2312 38-39 0.3730050601376931 2312 40-41 0.4575345667470003 2312 42-43 0.19475609009905526 2312 44-45 0.2693600742551041 2312 46-47 0.31064970291177474 2312 48-49 0.21172599178489548 2312 50-51 0.3405518199364721 2312 52-53 0.21574041602443828 2312 54-55 -0.15580314503040427 2312 56-57 0.15051832363366202 2312 58-59 0.16468422409059968 2312 60-61 0.1652190914753362 2312 62-63 0.13405638268445585 2312 64-65 0.21170608572480631 2312 66-67 0.1355967497234971 2312 68-69 0.17105621054134446 2312 70-71 0.1282765400116581 2312 72-73 0.0660291151324941 2312 74-75 0.016414642853533223 2312 76-77 0.13148464032278184 2312 78-79 0.14389772275812973 2312 80-81 -0.07672181679077283 2312 82-83 -0.1318450911404092 2312 84-85 -0.41962451800953815 2312 86-87 -0.553123766697535 2312 88-89 -0.2761122931959825 2312 90-91 -0.2519937728031145 2312 92-93 0.07785490254111593 2312 94-95 0.04891528522184885 2312 96-97 0.09776477525204896 2312 98-99 0.20083823880838025 2312 100-101 -0.1919530929077844 2313 1 0.032858807459621886 2313 2 0.050992511984290445 2313 3 0.1599594998882239 2313 4 0.17546136779388632 2313 5 0.13100382422982904 2313 6 0.08285272573154145 2313 7 0.20948147565550812 2313 8 0.12745088516295766 2313 9 0.17167103476936063 2313 10-11 0.24949407980584937 2313 12-13 0.21985882356879216 2313 14-15 0.3001847528877448 2313 16-17 0.26130723917121657 2313 18-19 -0.08702681773486631 2313 20-21 0.008003223679544647 2313 22-23 0.019866250771322314 2313 24-25 0.037304079790303035 2313 26-27 -0.12961840685186843 2313 28-29 -0.11158330498031432 2313 30-31 0.13552040031107992 2313 32-33 0.09117125446255159 2313 34-35 -0.041189804980788836 2313 36-37 0.02892633153071955 2313 38-39 0.10045113590973642 2313 40-41 0.1294765428356186 2313 42-43 0.20236024746646564 2313 44-45 0.2562173956579201 2313 46-47 0.17888874467086424 2313 48-49 0.25214941972455307 2313 50-51 0.08734228971761127 2313 52-53 0.3022125766331456 2313 54-55 0.1443826969841453 2313 56-57 0.5033639811967916 2313 58-59 0.3423456705852672 2313 60-61 0.22205876432472138 2313 62-63 0.23963018385325086 2313 64-65 0.18058540258157763 2313 66-67 0.3346781837199657 2313 68-69 0.07464712462654433 2313 70-71 -0.09245913684813445 2313 72-73 0.1798707828864572 2313 74-75 0.10888138872122965 2313 76-77 0.21188152676086247 2313 78-79 0.17321964404695223 2313 80-81 0.2184498960803083 2313 82-83 0.05668360664356342 2313 84-85 -0.09249227060016452 2313 86-87 0.10245887591582559 2313 88-89 0.12697972306540706 2313 90-91 0.08568580294488726 2313 92-93 0.40611138683939885 2313 94-95 0.3014044523062651 2313 96-97 0.25458693689082423 2313 98-99 0.5555853219900655 2313 100-101 0.5758635338150135 2314 1 -0.4568133430907402 2314 2 -0.3932288948883169 2314 3 -0.3515905849344385 2314 4 -0.21717365804743594 2314 5 -0.2823255638634379 2314 6 -0.06822629742701736 2314 7 -0.19914676437289813 2314 8 -0.16751972279853788 2314 9 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-1.1678835864669175 2314 74-75 -1.1448796086354562 2314 76-77 -0.9445827490903227 2314 78-79 -0.6848988709646839 2314 80-81 -0.6219162221979886 2314 82-83 -0.5877212538922016 2314 84-85 -0.8473709544680794 2314 86-87 -0.7571209560169514 2314 88-89 -0.8882974300493593 2314 90-91 -0.6377327475739101 2314 92-93 -0.6261354273792463 2314 94-95 -0.9767606165381721 2314 96-97 -1.1654675074221146 2314 98-99 -0.9626700928519298 2314 100-101 -1.2955812130978366 2315 1 -0.7381098616981845 2315 2 -1.1657939208987216 2315 3 -1.0217306409568465 2315 4 -0.6551968673311492 2315 5 -0.6582919504180609 2315 6 -0.3920998468467829 2315 7 -0.42800630819042595 2315 8 -0.19072100331074182 2315 9 -0.5552849870759928 2315 10-11 -0.736901436147015 2315 12-13 -0.7349069137120381 2315 14-15 -0.6927557472559727 2315 16-17 -0.876585833550827 2315 18-19 -0.9037413908880723 2315 20-21 -0.9472454109855946 2315 22-23 -0.840044643163715 2315 24-25 -1.1515691596463142 2315 26-27 -1.1215614939458547 2315 28-29 -1.1098615458541232 2315 30-31 -0.8254022786196487 2315 32-33 -1.0752673773281956 2315 34-35 -0.8236905235780867 2315 36-37 -1.0023010326543869 2315 38-39 -0.9551912900747439 2315 40-41 -1.1476174496909692 2315 42-43 -1.125662172769232 2315 44-45 -1.1857113194901032 2315 46-47 -1.5773026896493434 2315 48-49 -2.183426895308507 2315 50-51 -2.0258454078966466 2315 52-53 -1.691389033024798 2315 54-55 -1.791435066741066 2315 56-57 -1.9646124488118346 2315 58-59 -1.895064504752476 2315 60-61 -2.11727725177186 2315 62-63 -2.2712781231315162 2315 64-65 -1.8370231056104345 2315 66-67 -1.7550602468635326 2315 68-69 -1.855516715557421 2315 70-71 -1.5521142101450565 2315 72-73 -1.4972433303644763 2315 74-75 -1.6105178639375772 2315 76-77 -1.5496447739002477 2315 78-79 -1.558180183489693 2315 80-81 -1.9887389512896227 2315 82-83 -1.690094203071876 2315 84-85 -1.6339495859206572 2315 86-87 -1.6989696955127478 2315 88-89 -1.6635635364919388 2315 90-91 -1.4727907707831953 2315 92-93 -1.2419857675153025 2315 94-95 -1.5709582956098025 2315 96-97 -1.5757436178662907 2315 98-99 -1.8034143905710138 2315 100-101 -2.358554402373546 2316 1 -5.303692705004998 2316 2 -5.2508942322096885 2316 3 -5.979176161537961 2316 4 -7.947893166189672 2316 5 -8.58699655090232 2316 6 -9.267561624778295 2316 7 -11.362681161182469 2316 8 -11.531206994317316 2316 9 -11.380451019590698 2316 10-11 -11.232117624317194 2316 12-13 -9.903531092196985 2316 14-15 -9.818768372645117 2316 16-17 -8.725372585539759 2316 18-19 -9.2807778834512 2316 20-21 -10.4551310906812 2316 22-23 -10.052018873533829 2316 24-25 -10.11508610879886 2316 26-27 -7.949231850223963 2316 28-29 -6.287796516452531 2316 30-31 -8.550557934102176 2316 32-33 -6.078070040766448 2316 34-35 -4.654940955954427 2316 36-37 -5.151834930959474 2316 38-39 -4.3947727497772675 2316 40-41 -3.8761836897463198 2316 42-43 -3.9169420067367184 2316 44-45 -4.325994093616707 2316 46-47 -4.384048625311742 2316 48-49 -4.8150448475741605 2316 50-51 -4.805953501981737 2316 52-53 -4.808749807843206 2316 54-55 -4.279850839829962 2316 56-57 -4.683988097376346 2316 58-59 -5.081314937128816 2316 60-61 -5.469002433369923 2316 62-63 -4.934712056026708 2316 64-65 -5.709549048190855 2316 66-67 -5.03789922991438 2316 68-69 -5.375324740462297 2316 70-71 -4.80304468402953 2316 72-73 -3.791451216908925 2316 74-75 -4.04754743847926 2316 76-77 -4.702978395484472 2316 78-79 -4.886116883593463 2316 80-81 -5.155055969622381 2316 82-83 -4.727889083736006 2316 84-85 -4.606279229642553 2316 86-87 -5.179623448539385 2316 88-89 -6.778938839155376 2316 90-91 -5.939234907760021 2316 92-93 -5.384835992350997 2316 94-95 -4.7803581572493705 2316 96-97 -6.098999411709251 2316 98-99 -7.638375476700382 2316 100-101 -8.122780448724292 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8334.0 3 3231.0 4 3386.0 5 3730.0 6 3862.0 7 4599.0 8 5837.0 9 7237.0 10 8945.0 11 10596.0 12 12466.0 13 14297.0 14 15415.0 15 17911.0 16 20682.0 17 23775.0 18 21140.0 19 18814.0 20 19685.0 21 21439.0 22 23915.0 23 26829.0 24 31139.0 25 35546.0 26 41128.0 27 48167.0 28 57025.0 29 67038.0 30 80815.0 31 97664.0 32 118872.0 33 150162.0 34 193865.0 35 258278.0 36 351996.0 37 447243.0 38 329018.0 39 43846.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.20887972782884 18.00889046108401 14.25346052996581 23.52876928112134 2 15.570610609884582 19.232668208689045 40.17021176931013 25.026509412116248 3 19.731890199775492 23.20869838478425 29.102815711905095 27.956595703535164 4 13.024504778647142 15.973735760568285 36.66840170098837 34.33335775979621 5 14.462508971404828 36.51573301099233 33.856004230725645 15.165753786877195 6 33.03108054709983 35.034312736624905 17.312613472291794 14.621993243983466 7 29.67780704704962 31.571054411870307 20.14490179256443 18.606236748515645 8 26.610085721605426 34.415112334373966 19.377594489875964 19.597207454144648 9 26.208218759778752 17.001153419973363 20.22018530304107 36.57044251720682 10-11 25.498171539883668 25.89488739689818 27.108425653800843 21.4985154094173 12-13 26.276137096272993 23.131288879435427 27.529025161468134 23.063548862823442 14-15 23.023586250387527 24.81939601500866 25.67105297710596 26.48596475749786 16-17 22.807365267277472 27.99973774560606 26.28505125391799 22.907845733198478 18-19 22.57656965236732 26.746023109007133 28.328730878842084 22.348676359783465 20-21 23.2978781067706 25.822408088467903 28.392907292694197 22.486806512067297 22-23 22.924939478286685 25.739711403161387 28.372998449352405 22.962350669199523 24-25 23.01250757359418 25.99901562162399 28.293588017629666 22.694888787152163 26-27 22.788000855964675 26.14328040341396 28.42002934682076 22.648689393800602 28-29 22.55105923541681 26.38657064921307 28.414833922348233 22.647536193021896 30-31 22.73624807182871 26.00267267341551 28.280336514395028 22.98074274036075 32-33 22.57028368792987 26.11472570047632 28.370964699101727 22.944025912492087 34-35 23.26986373364561 26.18352796852902 27.940371926679763 22.60623637114561 36-37 23.062317527776 25.946013619808944 28.117391778073742 22.874277074341315 38-39 22.739408511261882 26.266083819417062 28.27171740519668 22.722790264124377 40-41 23.04399919556845 26.09811987729586 28.116393314154127 22.74148761298156 42-43 22.783058076742527 26.20109649445805 27.783826612329122 23.232018816470305 44-45 22.94568420671003 26.20184849947969 27.800794695784237 23.051672598026045 46-47 23.102869358345103 26.4474485979879 27.44113176857439 23.008550275092606 48-49 23.179805359735532 26.44983657869101 27.389105485249598 22.98125257632386 50-51 22.853071200737787 26.631222520905073 27.46850171568498 23.047204562672164 52-53 23.240227029227615 26.692871933324795 27.088953265952675 22.977947771494907 54-55 22.93612403469645 26.422839067326574 27.24661259814954 23.39442429982743 56-57 23.005460095417128 26.771821262365 27.160627641886474 23.0620910003314 58-59 23.26277050693785 26.427230303159625 27.1928385116942 23.11716067820832 60-61 23.156508681540387 26.461381931126272 27.62657479675595 22.75553459057739 62-63 22.920540073259076 26.593992967158798 27.49689088899237 22.988576070589755 64-65 22.803791577658174 26.65245174375449 27.269822764760963 23.273933913826372 66-67 22.81452953268042 26.799206625852413 27.013770373925905 23.37249346754126 68-69 22.720069641275675 26.576209800007934 27.402312437803428 23.301408120912964 70-71 22.92170174533868 26.54289853983269 27.21375560046777 23.321644114360858 72-73 22.913442203486472 26.440084996455965 27.37887240125187 23.267600398805698 74-75 22.704726918092614 26.908045547204402 27.065361454543257 23.321866080159726 76-77 22.922650769163354 26.645001760289382 27.038403772609897 23.393943697937363 78-79 22.902178531969746 26.69656364202952 27.12205256050691 23.27920526549383 80-81 22.96589681153903 26.931823460960153 26.977486751413803 23.124792976087015 82-83 22.963049966783984 26.833178551857205 27.108763854326902 23.095007627031908 84-85 22.940225530615564 26.726097397335835 26.907188092049967 23.426488979998634 86-87 22.86091296138694 26.772692845782096 27.081749137382293 23.284645055448667 88-89 22.86072953435267 26.71911588622583 26.898390400372822 23.521764179048674 90-91 22.86974591080573 26.720777589904866 27.058571152867827 23.350905346421573 92-93 23.04121818990764 26.44861549757409 27.251265831868523 23.25890048064975 94-95 22.803575165111926 26.461324566644933 27.240294787954973 23.494805480288168 96-97 22.705936296917958 26.462526632339074 27.38593842712739 23.445598643615583 98-99 22.61083808969792 26.552167655713493 27.041849712729427 23.795144541859155 100-101 22.568316124852924 26.419647696732873 27.170600977927695 23.841435200486508 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 117.0 1 104.0 2 122.5 3 170.0 4 171.5 5 176.5 6 288.5 7 575.5 8 988.0 9 1246.5 10 1223.5 11 1228.5 12 1351.0 13 1518.0 14 1743.0 15 1926.0 16 2114.0 17 2263.0 18 2429.0 19 2682.5 20 3025.0 21 3570.0 22 4284.0 23 5211.0 24 6285.5 25 8036.0 26 10664.0 27 13632.0 28 16600.5 29 20295.5 30 24980.0 31 30346.5 32 36466.5 33 41832.0 34 47122.5 35 52422.5 36 58697.5 37 64965.0 38 68754.0 39 71436.5 40 73371.0 41 77441.5 42 82521.5 43 86946.5 44 91071.5 45 95148.0 46 97850.0 47 101314.0 48 110512.5 49 111793.0 50 104432.0 51 99961.0 52 97284.5 53 96450.5 54 94931.5 55 89723.5 56 82350.0 57 74722.5 58 67729.0 59 59721.5 60 50032.5 61 41024.5 62 33685.0 63 26738.5 64 20662.5 65 16179.5 66 12626.0 67 10284.5 68 8013.5 69 5651.0 70 4100.5 71 3239.0 72 2509.5 73 1961.5 74 1553.5 75 1177.0 76 826.0 77 506.0 78 303.5 79 187.5 80 117.5 81 76.0 82 59.0 83 40.5 84 24.0 85 18.0 86 17.5 87 16.5 88 9.0 89 5.5 90 4.5 91 2.5 92 1.5 93 1.0 94 1.5 95 1.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07401974816668945 2 0.02904142160213479 3 0.013973115725344439 4 0.020393195923475663 5 0.024245244042354404 6 0.03096744566157416 7 0.07598353740376489 8 0.05543928076974495 9 0.07167830715325336 10-11 0.060027749852526985 12-13 0.039936675350139846 14-15 0.05047315991060227 16-17 0.06255801675414341 18-19 0.05770519142790891 20-21 0.019581244604300246 22-23 0.012783512629808357 24-25 0.022130394094734704 26-27 0.02403753556535604 28-29 0.006023545832952534 30-31 0.0011896030955360806 32-33 0.009875593951831272 34-35 0.011197375169093584 36-37 0.004059756595877101 38-39 0.009441294409016511 40-41 0.0041352869511492325 42-43 0.0018504937041672362 44-45 3.965343651786935E-4 46-47 5.853602533590237E-4 48-49 0.0011896030955360806 50-51 7.553035527213209E-4 52-53 3.776517763606605E-5 54-55 2.0770847699836325E-4 56-57 0.003568809286608241 58-59 0.00424858248405743 60-61 0.0052871248690492464 62-63 0.0029834490332492176 64-65 7.741861415393539E-4 66-67 4.154169539967265E-4 68-69 0.003285570454337746 70-71 0.002284793246981996 72-73 0.0202987829793855 74-75 0.015219366587334617 76-77 0.02454736546344293 78-79 0.019524596837846145 80-81 0.011367318468455881 82-83 0.004645116849236124 84-85 0.00368210481951644 86-87 0.001605020049532807 88-89 0.0017938459377131372 90-91 0.006457845375767294 92-93 0.010423189027554229 94-95 0.01299122110680672 96-97 0.011896030955360805 98-99 0.023112288713272422 100-101 0.019146945061485485 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2647942.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.804112398161735 #Duplication Level Percentage of deduplicated Percentage of total 1 79.7745071818085 47.708435940088364 2 11.978228467739182 14.326946432310692 3 3.5666592192474735 6.39902666501447 4 1.522550616586025 3.6421915282480435 5 0.8350761839503056 2.497049498299602 6 0.4950158486275724 1.7762390070116854 7 0.3268513469906958 1.3682938285045494 8 0.24890459008580287 1.1908414465527783 9 0.1735215632188581 0.9339572763220785 >10 0.9573790556298436 10.516136508132448 >50 0.077441901557066 3.2263084331478447 >100 0.04113339117086874 4.458050316169725 >500 0.0018415899591940066 0.7311421854589368 >1k 8.255403265161093E-4 1.0070484797146042 >5k 6.350310203970071E-5 0.21833245502429605 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5749 0.21711200622974372 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4714 0.17802504737641534 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4118 0.15551700150532 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2869 0.10834829463787349 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2773 0.10472283758481114 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.776517763606605E-5 0.0 12-13 0.0 0.0 0.0 3.776517763606605E-5 0.0 14-15 0.0 0.0 0.0 3.776517763606605E-5 0.0 16-17 0.0 0.0 0.0 1.1329553290819814E-4 0.0 18-19 0.0 0.0 0.0 1.1329553290819814E-4 0.0 20-21 0.0 0.0 0.0 1.5106071054426417E-4 0.0 22-23 0.0 0.0 0.0 2.2659106581639628E-4 0.0 24-25 0.0 0.0 0.0 4.154169539967265E-4 0.0 26-27 0.0 0.0 0.0 5.098298980868916E-4 3.776517763606605E-5 28-29 0.0 0.0 0.0 0.0011896030955360804 3.776517763606605E-5 30-31 0.0 0.0 0.0 0.0023603236022541278 3.776517763606605E-5 32-33 0.0 0.0 0.0 0.004021991418241035 3.776517763606605E-5 34-35 0.0 0.0 0.0 0.005551481112501709 7.55303552721321E-5 36-37 0.0 0.0 0.0 0.007741861415393539 7.55303552721321E-5 38-39 0.0 0.0 0.0 0.011631674711908343 7.55303552721321E-5 40-41 0.0 0.0 0.0 0.01608796567296414 7.55303552721321E-5 42-43 0.0 0.0 0.0 0.019524596837846145 7.55303552721321E-5 44-45 0.0 0.0 0.0 0.021809390084828142 7.55303552721321E-5 46-47 0.0 0.0 0.0 0.025000547595075723 7.55303552721321E-5 48-49 0.0 0.0 0.0 0.02947572114494955 7.55303552721321E-5 50-51 0.0 0.0 0.0 0.0341963683494578 7.55303552721321E-5 52-53 0.0 0.0 0.0 0.042806828850480864 7.55303552721321E-5 54-55 0.0 0.0 0.0 0.050095508134241615 7.55303552721321E-5 56-57 0.0 0.0 0.0 0.06622123898484182 7.55303552721321E-5 58-59 0.0 0.0 0.0 0.0955458994192471 9.441294409016511E-5 60-61 0.0 0.0 0.0 0.11846936224433918 1.1329553290819814E-4 62-63 0.0 0.0 0.0 0.13293342527895247 1.1329553290819814E-4 64-65 0.0 0.0 0.0 0.16116289556191185 1.1329553290819814E-4 66-67 0.0 0.0 0.0 0.2168287673974732 1.1329553290819814E-4 68-69 0.0 0.0 0.0 0.2898288557679889 1.1329553290819814E-4 70-71 0.0 0.0 0.0 0.35316105866367165 1.1329553290819814E-4 72-73 0.0 0.0 0.0 0.42774728449490207 1.1329553290819814E-4 74-75 0.0 0.0 0.0 0.5150226100118507 1.1329553290819814E-4 76-77 0.0 0.0 0.0 0.5764287888480941 1.1329553290819814E-4 78-79 0.0 0.0 0.0 0.654678236910023 1.1329553290819814E-4 80-81 0.0 0.0 0.0 0.7586080057644766 1.1329553290819814E-4 82-83 0.0 0.0 0.0 0.854890326147627 1.1329553290819814E-4 84-85 0.0 0.0 1.8882588818033024E-5 0.993167524062083 1.1329553290819814E-4 86-87 0.0 0.0 3.776517763606605E-5 1.200800470705174 1.1329553290819814E-4 88-89 0.0 0.0 3.776517763606605E-5 1.46502453603591 1.1329553290819814E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12795 0.0 24.40206 1 GGTATCA 9415 0.0 23.62256 1 GTACATG 9300 0.0 23.556969 1 TACATGG 9455 0.0 22.617052 2 ACATGGG 9380 0.0 22.139704 3 GAGTACT 6785 0.0 21.574629 12-13 TCAACGC 14650 0.0 21.14925 4 ATCAACG 14730 0.0 21.002522 3 CAACGCA 14820 0.0 20.843306 5 TATCAAC 14970 0.0 20.665419 2 AACGCAG 15125 0.0 20.391188 6 GTACTTT 7290 0.0 19.815752 14-15 AGAGTAC 11885 0.0 19.176477 10-11 GTATAGG 800 0.0 19.009094 1 GTACCTG 2230 0.0 18.966473 1 GTGTAGC 1390 0.0 18.80396 1 AGTACTT 7370 0.0 18.733593 12-13 ACGCAGA 16785 0.0 18.232983 7 CGCAGAG 16955 0.0 18.194092 8 CATGGGG 5820 0.0 17.636637 4 >>END_MODULE