FastQCFastQC Report
Thu 26 May 2016
SRR936624_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936624_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647942
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69830.2637142354326492No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62040.23429516205415377No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52890.1997400245171533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37470.14150612060233947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113800.023.7980461
GTATCAA149200.022.48251
GTACATG91600.022.4595551
TACATGG93950.021.2355142
GAGTACT89100.021.13538412-13
ACATGGG90150.020.9167423
ACTGCGT3451.8189894E-1220.6486768
TAGACGC1656.3331085E-520.1488634
GTACTTT95750.019.5675514-15
AGAGTAC138000.019.53020710-11
TCAACGC175300.019.0191754
AGTACTT95150.019.01782612-13
ATCAACG175750.018.9719093
TATCAAC177700.018.7922232
CAACGCA177750.018.7570275
AACGCAG181300.018.4407466
GTACCTG22100.018.2740751
CATGGGG56900.017.444984
TACCTGG22200.017.1177752
ACTTTTT111000.016.9006116-17
Produced by FastQC (version 0.11.5)