##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936624_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2647942 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8950996660803 31.0 31.0 34.0 30.0 34.0 2 32.07069452427584 33.0 31.0 34.0 30.0 34.0 3 32.158576358545616 33.0 31.0 34.0 30.0 34.0 4 35.84469183992701 37.0 35.0 37.0 35.0 37.0 5 35.671144987314676 37.0 35.0 37.0 33.0 37.0 6 35.75299685567131 37.0 35.0 37.0 33.0 37.0 7 35.69985558596072 37.0 35.0 37.0 33.0 37.0 8 35.667161516377625 37.0 35.0 37.0 33.0 37.0 9 37.30947165761184 39.0 37.0 39.0 34.0 39.0 10-11 37.19744295758744 39.0 37.0 39.0 33.0 39.0 12-13 37.0932493989672 39.0 37.0 39.0 33.0 39.0 14-15 38.252968531788085 40.0 38.0 41.0 33.0 41.0 16-17 38.149948526062886 40.0 37.0 41.0 33.0 41.0 18-19 38.273809245066545 40.0 38.0 41.0 33.5 41.0 20-21 38.28468542739984 40.0 38.0 41.0 34.0 41.0 22-23 38.20053913567593 40.0 38.0 41.0 33.0 41.0 24-25 38.07302388043243 40.0 37.0 41.0 33.0 41.0 26-27 37.88844374234783 40.0 37.0 41.0 33.0 41.0 28-29 37.81134650985558 40.0 37.0 41.0 33.0 41.0 30-31 37.80263823754448 40.0 37.0 41.0 33.0 41.0 32-33 37.676167000636724 40.0 37.0 41.0 33.0 41.0 34-35 37.51768675446819 40.0 36.5 41.0 32.0 41.0 36-37 37.33748435577516 39.5 36.0 41.0 31.5 41.0 38-39 37.18526142188915 39.0 36.0 41.0 31.0 41.0 40-41 37.012688910859836 39.0 36.0 41.0 31.0 41.0 42-43 36.7581652090567 39.0 35.0 41.0 30.5 41.0 44-45 36.51548485578611 39.0 35.0 40.0 30.0 41.0 46-47 36.19628243367869 39.0 35.0 40.0 30.0 41.0 48-49 35.85884811676389 39.0 35.0 40.0 29.0 41.0 50-51 35.58052083467085 38.0 35.0 40.0 28.0 41.0 52-53 35.22683144117205 38.0 34.0 40.0 27.0 41.0 54-55 35.03269614666787 38.0 34.0 40.0 27.0 41.0 56-57 34.72982187676316 38.0 34.0 40.0 26.0 41.0 58-59 34.36021087319888 37.5 33.0 40.0 26.0 41.0 60-61 33.93682659967628 37.0 33.0 40.0 25.0 41.0 62-63 33.602079463976175 36.0 32.5 39.0 24.0 40.5 64-65 33.18297700629394 36.0 32.0 39.0 23.0 40.0 66-67 32.75846770812956 35.0 31.5 38.5 22.5 40.0 68-69 32.40006559811356 35.0 31.0 38.0 22.0 40.0 70-71 32.03171311909399 35.0 31.0 37.5 21.5 39.5 72-73 31.575881382598258 35.0 31.0 37.0 20.0 39.0 74-75 31.07026022473302 34.0 30.0 36.5 18.5 39.0 76-77 29.570298178736543 32.5 28.5 35.0 17.5 37.0 78-79 30.343456729792422 34.0 30.0 35.0 18.0 37.0 80-81 30.12004889079897 34.0 30.0 35.0 14.0 37.0 82-83 29.82976402051102 34.0 29.0 35.0 12.0 36.5 84-85 29.456627637614417 34.0 29.0 35.0 7.5 36.0 86-87 29.083433851647808 34.0 29.0 35.0 6.0 36.0 88-89 28.629316843042634 33.0 29.0 35.0 2.0 35.0 90-91 28.16002370897852 33.0 27.5 35.0 2.0 35.0 92-93 27.53914908257054 33.0 26.5 35.0 2.0 35.0 94-95 26.997879485275735 32.5 25.5 34.0 2.0 35.0 96-97 26.282226914335737 32.0 25.0 34.0 2.0 35.0 98-99 25.401903251657323 32.0 22.0 34.0 2.0 35.0 100-101 22.164308734859 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30440347109920296 1101 2 0.29414818909534546 1101 3 0.2652730548046094 1101 4 0.28305783450237243 1101 5 0.236136702056001 1101 6 0.25991317407875414 1101 7 0.26735149415683424 1101 8 0.22492159568058412 1101 9 0.2937673065495403 1101 10-11 0.30716483147113394 1101 12-13 0.34189885434349065 1101 14-15 0.5358077836024862 1101 16-17 0.5043138002265053 1101 18-19 0.4246674574071463 1101 20-21 0.4211676274196776 1101 22-23 0.39836703083367553 1101 24-25 0.29985836169077373 1101 26-27 0.40308648687591386 1101 28-29 0.4070001886412413 1101 30-31 0.4534501105005191 1101 32-33 0.4230905444182369 1101 34-35 0.43105903783917654 1101 36-37 0.486947790191401 1101 38-39 0.48684224032705714 1101 40-41 0.5382921244215808 1101 42-43 0.5639196236733355 1101 44-45 0.5237713455991084 1101 46-47 0.48822851505026676 1101 48-49 0.46810948548095865 1101 50-51 0.40111351657395033 1101 52-53 0.5047923861584067 1101 54-55 0.43953321649314603 1101 56-57 0.41705668437966636 1101 58-59 0.3781747727751892 1101 60-61 0.43791132641537445 1101 62-63 0.47180948257650357 1101 64-65 0.43268325577573563 1101 66-67 0.3219020106424608 1101 68-69 0.26706386092780576 1101 70-71 0.3461075350923153 1101 72-73 0.5558653503113753 1101 74-75 0.45947682060294426 1101 76-77 0.2556103748313241 1101 78-79 0.35458906741702023 1101 80-81 0.5206092521518464 1101 82-83 0.5527810618211824 1101 84-85 0.572975235021481 1101 86-87 0.44165454448413755 1101 88-89 0.5147945853929699 1101 90-91 0.6321997621393471 1101 92-93 0.6609218548854976 1101 94-95 0.5890975581237932 1101 96-97 0.7491757751105901 1101 98-99 0.5803400429675563 1101 100-101 0.9730503518455187 1102 1 0.1464177043584307 1102 2 0.16615779551314347 1102 3 0.15778594129844947 1102 4 0.15315286650432114 1102 5 0.14621195217918626 1102 6 0.15703281944100667 1102 7 0.1898955433925309 1102 8 0.1758761790359813 1102 9 0.24576786571970644 1102 10-11 0.27732344327510106 1102 12-13 0.29138006245621995 1102 14-15 0.28691770945633976 1102 16-17 0.29869866143472024 1102 18-19 0.24841778388892521 1102 20-21 0.2821234134845838 1102 22-23 0.2012399134142271 1102 24-25 0.15360692225393535 1102 26-27 0.22575678695194057 1102 28-29 0.2121794237718433 1102 30-31 0.335233021883262 1102 32-33 0.3169490182696322 1102 34-35 0.25975618032704517 1102 36-37 0.14593942373119972 1102 38-39 -0.04684523785139305 1102 40-41 0.18125843913117023 1102 42-43 0.20563975406858503 1102 44-45 0.11930008227842848 1102 46-47 0.06697317129523839 1102 48-49 0.17535007719607876 1102 50-51 0.0832127113868566 1102 52-53 0.12279933719107561 1102 54-55 0.10268109770494505 1102 56-57 -0.09035832265259813 1102 58-59 0.15168426565362125 1102 60-61 0.17167979675735978 1102 62-63 0.1788156469115023 1102 64-65 -0.01928291622429157 1102 66-67 -0.07197660186159283 1102 68-69 0.07031107562311689 1102 70-71 0.06596244030713905 1102 72-73 0.24364196227869073 1102 74-75 -0.06900590974509768 1102 76-77 7.239747832130661E-4 1102 78-79 0.1476625601482553 1102 80-81 0.24173895090901354 1102 82-83 0.17244381399750353 1102 84-85 0.13364200056762243 1102 86-87 0.18763191873592433 1102 88-89 0.03854748109561967 1102 90-91 -0.042799071052922244 1102 92-93 0.053706192466311364 1102 94-95 0.023501254301418584 1102 96-97 0.2103396456272435 1102 98-99 0.1277384095264864 1102 100-101 0.7529797554900846 1103 1 0.1667193846210786 1103 2 0.19060726577163933 1103 3 0.17199183081974923 1103 4 0.130114666335146 1103 5 0.1407353281881285 1103 6 0.17735690081618571 1103 7 0.06488299876951942 1103 8 0.04296500213529697 1103 9 0.1631023842212329 1103 10-11 0.16197025837453083 1103 12-13 0.19581378025237228 1103 14-15 0.23221374463863498 1103 16-17 0.22582425820036178 1103 18-19 0.21395501633003988 1103 20-21 0.09363194024406596 1103 22-23 0.07752663331788057 1103 24-25 0.006472830986425038 1103 26-27 0.1181888846996415 1103 28-29 0.05820627284927582 1103 30-31 0.1231581053946087 1103 32-33 0.06515065695581512 1103 34-35 0.10294391240256573 1103 36-37 0.22038638506509045 1103 38-39 0.21435083819053347 1103 40-41 0.4237580986342593 1103 42-43 0.41218562796287017 1103 44-45 0.30064425655550053 1103 46-47 0.49097246879635037 1103 48-49 0.31202329693486774 1103 50-51 0.3635778674419683 1103 52-53 0.38328350587683246 1103 54-55 0.22187876153771668 1103 56-57 0.1345958894733954 1103 58-59 0.3654450898711943 1103 60-61 0.1458741631463738 1103 62-63 0.14833221298223975 1103 64-65 0.0610071513016166 1103 66-67 0.008139633031099436 1103 68-69 0.1405098899770323 1103 70-71 0.10271012134397495 1103 72-73 0.06153301401988642 1103 74-75 -0.0769578566705853 1103 76-77 0.08414258168490463 1103 78-79 0.08457385891099634 1103 80-81 0.2747586387987937 1103 82-83 0.14944235165287623 1103 84-85 0.4531392120771365 1103 86-87 0.4124118502064391 1103 88-89 0.19155559209044526 1103 90-91 0.30755432889833756 1103 92-93 0.33624022423646593 1103 94-95 0.15663285814939698 1103 96-97 0.19430000103433542 1103 98-99 -0.003682197346531524 1103 100-101 0.248780442935427 1104 1 0.16310057002834455 1104 2 0.20008447003241514 1104 3 0.15475088641453993 1104 4 0.14805198706103795 1104 5 0.13838584637139206 1104 6 0.0695600084743404 1104 7 0.12792622635214457 1104 8 0.11568092383845396 1104 9 0.1459263028109632 1104 10-11 0.20261773520402215 1104 12-13 0.1553856664682698 1104 14-15 0.21948122881926935 1104 16-17 0.282047862564923 1104 18-19 0.13730767643352948 1104 20-21 0.16973560212047545 1104 22-23 0.14086264525267467 1104 24-25 0.18896756987683005 1104 26-27 0.0902063580697714 1104 28-29 0.12487326983674762 1104 30-31 0.1455619640221144 1104 32-33 0.0879265769292914 1104 34-35 0.10202862347396291 1104 36-37 0.012856331877429739 1104 38-39 0.10359367082958926 1104 40-41 0.048806295443000636 1104 42-43 -0.0894985577182652 1104 44-45 0.09650239397856808 1104 46-47 0.061510975794355716 1104 48-49 0.11289975815400766 1104 50-51 0.09628377141591216 1104 52-53 0.11311697013731958 1104 54-55 0.11421114474829608 1104 56-57 -0.015434470011733481 1104 58-59 -0.11858014543733475 1104 60-61 -0.06267973439892671 1104 62-63 -0.11804659411521357 1104 64-65 -0.12607394185605614 1104 66-67 -0.21564760913849312 1104 68-69 -0.22974610264299855 1104 70-71 -0.06742641526037829 1104 72-73 0.07930232157137951 1104 74-75 0.05737025278932251 1104 76-77 -0.1010876639296363 1104 78-79 -0.048589869702230004 1104 80-81 0.07762513273770466 1104 82-83 0.04951280007238168 1104 84-85 0.3056867062034314 1104 86-87 0.24707157172797878 1104 88-89 0.13049473979069148 1104 90-91 0.18243180990789298 1104 92-93 0.2401335354202878 1104 94-95 0.34150680772922826 1104 96-97 0.6129613549412483 1104 98-99 0.5242751047852963 1104 100-101 1.2371875339021763 1105 1 0.027527105859732615 1105 2 0.04280490947110138 1105 3 0.1363314696517861 1105 4 0.17142557988086082 1105 5 0.10486820192175372 1105 6 0.1459142281513479 1105 7 0.09647903362655086 1105 8 0.12120151493464704 1105 9 0.13952134964718255 1105 10-11 0.17541238605585363 1105 12-13 0.18389403477883803 1105 14-15 0.20846023465274044 1105 16-17 0.16566314574023266 1105 18-19 0.0996551101730887 1105 20-21 0.16406506748172944 1105 22-23 0.1919047039849957 1105 24-25 0.19469970387655167 1105 26-27 0.04678674575349362 1105 28-29 -0.009469529974417412 1105 30-31 0.12375078636656411 1105 32-33 0.17583593948000953 1105 34-35 0.25497753615034213 1105 36-37 0.2564937103006528 1105 38-39 0.1571097692560457 1105 40-41 0.19915089526686103 1105 42-43 0.20671994320981213 1105 44-45 0.37649121751934445 1105 46-47 0.15779664360734813 1105 48-49 0.31720971187453273 1105 50-51 0.2388592596785557 1105 52-53 0.45383062777969485 1105 54-55 0.3360087026758478 1105 56-57 0.1271021951242446 1105 58-59 0.1267618845831251 1105 60-61 0.45979327691373584 1105 62-63 0.3779174686680449 1105 64-65 0.4003981390819362 1105 66-67 0.3463859956998192 1105 68-69 0.40398225648346653 1105 70-71 0.5860333522471848 1105 72-73 0.5733768771587329 1105 74-75 0.46106290154113694 1105 76-77 0.3021337583380692 1105 78-79 0.3420495811282471 1105 80-81 0.2617449325092984 1105 82-83 0.6324182354481529 1105 84-85 0.5895586476312396 1105 86-87 0.6319693409453713 1105 88-89 0.5804553530097394 1105 90-91 0.7687767442433184 1105 92-93 0.5704439754939408 1105 94-95 0.8001043300430339 1105 96-97 0.7949881568174604 1105 98-99 0.45069740009597226 1105 100-101 0.8796671434208925 1106 1 0.044204513712429616 1106 2 0.08505162780594588 1106 3 -0.0016454970151826842 1106 4 0.07795476883686803 1106 5 0.09379369186899567 1106 6 -0.06977738979326631 1106 7 -0.0046366449220300865 1106 8 0.0504871416177437 1106 9 -0.05062984300328566 1106 10-11 0.02436197145870267 1106 12-13 0.028612709621121724 1106 14-15 0.025190346135367747 1106 16-17 -0.05939680286306981 1106 18-19 0.03462010937519722 1106 20-21 -0.01509187805642398 1106 22-23 -0.22598731418521112 1106 24-25 -0.10018468197463193 1106 26-27 -0.1464555763504407 1106 28-29 -0.27591147619208556 1106 30-31 -0.15823026637068693 1106 32-33 -0.1182074612252606 1106 34-35 -0.04909304977859108 1106 36-37 -0.22214877109799858 1106 38-39 0.07434642512786382 1106 40-41 0.13670668274574638 1106 42-43 -0.05634557313187116 1106 44-45 -0.08147041632159358 1106 46-47 -0.038201491167150436 1106 48-49 -0.27717364618484197 1106 50-51 -0.33275706547205885 1106 52-53 -0.559970395418901 1106 54-55 -0.3306554582402157 1106 56-57 -0.4644178002920114 1106 58-59 -0.32539785145360867 1106 60-61 -0.332522458079346 1106 62-63 -0.29601776884632613 1106 64-65 -0.3213281747087464 1106 66-67 -0.18045343438121364 1106 68-69 -0.3130750744588724 1106 70-71 -0.0738133123896283 1106 72-73 0.12024552152951173 1106 74-75 -0.025350287341002087 1106 76-77 -0.22339388170892605 1106 78-79 -0.3055214113241931 1106 80-81 -0.12691095445774891 1106 82-83 -0.17386177140852865 1106 84-85 0.18931269735827172 1106 86-87 0.14653641353574187 1106 88-89 -1.1526204114886696E-4 1106 90-91 -0.025363966398600724 1106 92-93 0.09175307589080361 1106 94-95 0.0642876676460098 1106 96-97 0.32160272044513505 1106 98-99 0.2748017580184907 1106 100-101 0.8016289543611812 1107 1 0.10669636280861994 1107 2 0.10204072915512086 1107 3 0.06679068128899246 1107 4 0.14436428737904805 1107 5 0.06586810045845226 1107 6 -8.547750689942291E-4 1107 7 0.10086960592875727 1107 8 0.06824564069008687 1107 9 0.10092651555842025 1107 10-11 0.23041931860010578 1107 12-13 0.06992339399242553 1107 14-15 0.1548054235701457 1107 16-17 0.27432559234605236 1107 18-19 0.12006680746191023 1107 20-21 0.19172594246090569 1107 22-23 -0.004469189906892268 1107 24-25 0.13201993165110792 1107 26-27 0.1282542752721625 1107 28-29 0.2105123308249759 1107 30-31 0.09140883302702463 1107 32-33 0.12544459108902828 1107 34-35 0.15186159359497253 1107 36-37 0.06884730028536268 1107 38-39 0.09411623408495728 1107 40-41 -0.018429647962477702 1107 42-43 0.030881353741790463 1107 44-45 0.14590767233548974 1107 46-47 -0.06649512265777702 1107 48-49 -0.10342648174742664 1107 50-51 0.006870986773684251 1107 52-53 0.19590313396766135 1107 54-55 0.015526039660301194 1107 56-57 -0.08908902733977442 1107 58-59 -0.09510138225855513 1107 60-61 0.126970286783326 1107 62-63 -0.04156654242683544 1107 64-65 -0.02181173153946503 1107 66-67 0.02696196042714405 1107 68-69 0.26311136396027734 1107 70-71 0.16747949948894103 1107 72-73 0.2562491226821102 1107 74-75 0.2176042379931964 1107 76-77 0.1373406087275093 1107 78-79 0.06149653351524975 1107 80-81 0.3595047611511468 1107 82-83 0.31294768640048787 1107 84-85 0.5205459136359032 1107 86-87 0.4872669201435684 1107 88-89 0.354263903249187 1107 90-91 0.3291974935579738 1107 92-93 0.42853771211537506 1107 94-95 0.47425266516622955 1107 96-97 0.712370495127022 1107 98-99 0.57946850147421 1107 100-101 0.9818105731568529 1108 1 0.03228477684811892 1108 2 0.06732839087164422 1108 3 0.022596066787578195 1108 4 0.07453601039173918 1108 5 0.07844849505831775 1108 6 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2316 48-49 -1.291492859142359 2316 50-51 -1.1449646867137417 2316 52-53 -1.2355707268794447 2316 54-55 -1.0519265271417026 2316 56-57 -0.9890507841421226 2316 58-59 -1.5676573074822002 2316 60-61 -1.1556864957859219 2316 62-63 -0.9347005070395227 2316 64-65 -1.0060518988207363 2316 66-67 -1.054267463860043 2316 68-69 -1.473849305164812 2316 70-71 -1.3524811231045035 2316 72-73 -0.8379860424470742 2316 74-75 -0.907228975133382 2316 76-77 -0.9299543321191344 2316 78-79 -1.1025964483490398 2316 80-81 -0.8915248969575735 2316 82-83 -1.3587047978078317 2316 84-85 -1.1214347931798194 2316 86-87 -1.1883177790874981 2316 88-89 -1.4165037526027504 2316 90-91 -1.5127461333512784 2316 92-93 -1.6463625356656486 2316 94-95 -1.694177211641236 2316 96-97 -1.6982331948326106 2316 98-99 -2.230560086406623 2316 100-101 -3.026461445037018 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 10.0 4 36.0 5 68.0 6 177.0 7 397.0 8 945.0 9 1771.0 10 2904.0 11 4528.0 12 6289.0 13 8692.0 14 11209.0 15 14638.0 16 18339.0 17 22644.0 18 20849.0 19 15803.0 20 15273.0 21 16030.0 22 17303.0 23 19679.0 24 23194.0 25 27796.0 26 34698.0 27 42769.0 28 53040.0 29 66022.0 30 81112.0 31 100283.0 32 124603.0 33 159399.0 34 208147.0 35 281585.0 36 397415.0 37 519311.0 38 313121.0 39 17856.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.68193505520726 19.185559194783387 14.87919975432379 23.253305995685558 2 15.941466812107002 20.1793554293864 39.24505739555037 24.634120362956228 3 19.896325699707106 23.437718938783064 29.390474427950135 27.27548093355969 4 13.49126982388587 16.73952828271918 36.12628222219369 33.64291967120126 5 14.338619902547055 36.79849477423502 33.836157897638905 15.026727425579017 6 33.064243854283816 35.70988337357842 16.75550295285924 14.47036981927852 7 29.649403196897815 31.941447357985936 19.8358574319226 18.573292013193644 8 26.78876652132109 34.92954150808439 18.885874388487363 19.39581758210716 9 26.13085936172318 16.97612712060914 19.941750990014132 36.95126252765355 10-11 25.583698585543036 26.27572658313513 26.72364047248769 21.416934358834144 12-13 26.38427545039759 23.343670414842194 27.40509922824053 22.866954906519688 14-15 23.003964973486905 25.194292764518188 25.571643131992943 26.23009913000196 16-17 22.757428221615125 28.36331007250159 26.226518556675334 22.65274314920795 18-19 22.540107795039493 26.960391752620144 28.4720261807915 22.027474271548858 20-21 23.25511661509202 25.92194239904046 28.65723645004309 22.165704535824425 22-23 22.877816082208856 25.855453213886953 28.596359016646346 22.67037168725784 24-25 23.05161895540008 26.08933654891232 28.574606241375378 22.284438254312217 26-27 22.778431544346017 26.241491383373415 28.623224255864262 22.356852816416307 28-29 22.51283156187503 26.556050524469615 28.580398060248907 22.35071985340645 30-31 22.743560901248173 26.096588416769407 28.49459695597143 22.66525372601099 32-33 22.626090457453937 26.2645491894603 28.546455810271542 22.562904542814216 34-35 23.16475480244594 26.266444427281964 28.266636655411816 22.302164114860272 36-37 23.12403108094074 25.991465015058964 28.387057790534097 22.497446113466204 38-39 22.80396018215886 26.33980743669957 28.459971332090966 22.3962610490506 40-41 23.027048236815393 26.201143078986807 28.294301674964956 22.477507009232852 42-43 22.889503189134754 26.397107656737045 27.86352236756409 22.84986678656411 44-45 23.026672825003654 26.36333458086235 27.868092500437136 22.741900093696856 46-47 23.228892120759852 26.71461098147121 27.392120571026535 22.664376326742406 48-49 23.332822871027574 26.702874872093314 27.301966274366002 22.66233598251311 50-51 23.06562401987507 26.81166740717928 27.35592057298871 22.766787999956946 52-53 23.486557076908205 26.95861984579688 26.9045211062503 22.650301971044616 54-55 23.13804812732742 26.724343525572113 27.11487743085263 23.02273091624783 56-57 23.16545428415547 27.02193166253118 27.029504528706166 22.78310952460719 58-59 23.47222426830968 26.717117517316506 27.02085762668712 22.789800587686685 60-61 23.332434360843777 26.817576951856246 27.40317443050282 22.446814256797158 62-63 23.100302458951997 26.873428784664938 27.341469660436196 22.684799095946868 64-65 22.983619362451446 26.905918604457913 27.152054982859404 22.958407050231237 66-67 23.037745219297665 27.02334127048569 26.87580842823612 23.063105081980524 68-69 22.940412497200636 26.73006483199461 27.22532129923816 23.1042013715666 70-71 23.111748420186416 26.789287819696757 27.134197577814295 22.964766182302537 72-73 23.196117285090565 26.68090036749308 27.114331474908237 23.00865087250811 74-75 22.972320748788473 27.219189215318984 26.797195064473637 23.011294971418902 76-77 23.114526326443443 26.98710180071748 26.826388870530447 23.07198300230863 78-79 23.137554308487505 26.97506138768749 26.847215615388553 23.040168688436456 80-81 23.245751534252122 27.204307086114525 26.668149802231806 22.881791577401547 82-83 23.19368361970728 27.163611805574178 26.77247350265096 22.870231072067575 84-85 23.12517966914058 27.14425321289032 26.609656066517907 23.1209110514512 86-87 23.06382446741812 27.076412148350926 26.737207988312733 23.12255539591822 88-89 23.011633890231746 27.167120904960555 26.478131958554123 23.343113246253573 90-91 23.099964258268393 27.094348348135043 26.625417585083078 23.180269808513483 92-93 23.219858975840943 26.890087133377605 26.742913094031973 23.14714079674948 94-95 23.008748598029516 26.86060229671947 26.83499001007492 23.295659095176088 96-97 22.96296450227965 26.857338466310775 26.92209891875737 23.257598112652207 98-99 22.754131164630518 26.988720143292174 26.497036503722388 23.760112188354924 100-101 22.637242801114013 27.184396866994636 26.49963526866086 23.67872506323049 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 243.0 1 189.5 2 164.0 3 200.5 4 183.0 5 163.5 6 272.0 7 607.5 8 1052.5 9 1351.0 10 1389.0 11 1397.0 12 1600.5 13 1815.0 14 2010.0 15 2275.0 16 2524.0 17 2736.0 18 2977.5 19 3305.5 20 3665.5 21 4119.5 22 4829.5 23 5893.5 24 7142.0 25 8807.0 26 11241.0 27 14229.0 28 17413.0 29 21279.5 30 25991.0 31 30854.5 32 36753.0 33 42104.5 34 47128.5 35 52452.5 36 58846.0 37 65124.5 38 68877.5 39 71538.5 40 73258.0 41 76879.5 42 81986.5 43 86607.5 44 90736.0 45 94217.0 46 96087.0 47 100772.0 48 111562.0 49 112025.0 50 103758.5 51 99473.0 52 96386.0 53 95072.5 54 94169.5 55 89274.0 56 81419.5 57 73807.5 58 67455.0 59 59724.0 60 49784.5 61 40784.5 62 33345.0 63 26460.5 64 20375.0 65 15732.0 66 12173.0 67 10197.5 68 7927.0 69 5351.5 70 3890.0 71 3031.5 72 2493.5 73 2026.0 74 1590.5 75 1237.5 76 855.0 77 510.5 78 308.5 79 192.5 80 135.5 81 87.0 82 55.0 83 38.0 84 25.0 85 17.0 86 7.5 87 3.0 88 3.0 89 3.5 90 1.5 91 1.0 92 1.0 93 1.5 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020959673588016654 2 0.013217812172623116 3 0.008572695323386992 4 0.0 5 0.0037765177636066047 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0011896030955360806 14-15 1.8882588818033022E-4 16-17 0.0 18-19 0.001756080760077071 20-21 0.0 22-23 1.3217812172623117E-4 24-25 0.0 26-27 0.0014350767501705097 28-29 0.0028890360891590527 30-31 0.0013784289837164107 32-33 0.007553035527213209 34-35 0.001756080760077071 36-37 6.420080198131227E-4 38-39 0.0012651334508082125 40-41 2.2659106581639628E-4 42-43 0.0046639994380541575 44-45 0.0015483722830787077 46-47 3.3988659872459444E-4 48-49 5.853602533590237E-4 50-51 5.664776645409907E-5 52-53 2.2659106581639628E-4 54-55 0.0010763075626278823 56-57 0.012519156386355895 58-59 0.023150053890908486 60-61 0.01793845937713137 62-63 0.011707205067180475 64-65 0.016087965672964135 66-67 0.0021526151252557646 68-69 7.93068730357387E-4 70-71 1.1329553290819814E-4 72-73 3.776517763606605E-5 74-75 0.0014350767501705097 76-77 0.0020015544147115004 78-79 0.008327221668752563 80-81 0.015030540699154287 82-83 0.015597018363695278 84-85 0.02700210200978722 86-87 0.010347658672282097 88-89 0.03852048118878736 90-91 0.04441184890001367 92-93 0.02779517074014461 94-95 0.029268012667951183 96-97 0.04777294970962355 98-99 0.049906682246061276 100-101 0.055646989246743314 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2647942.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.6870127579616 #Duplication Level Percentage of deduplicated Percentage of total 1 77.62392214911713 44.002682651900216 2 13.01329993700092 14.753701991039039 3 4.072450528808693 6.925651652482374 4 1.768736147479811 4.010574742306235 5 0.9183736294211104 2.6029928823784996 6 0.5824495634132627 1.9810395499246074 7 0.3660450212198951 1.4524999151516362 8 0.2587727505280114 1.1735243368475357 9 0.20279412274078046 1.0346213720741626 >10 1.0513516276594905 11.056558893576963 >50 0.09121958602727556 3.601224749410699 >100 0.04704650952031851 4.946235548241151 >500 0.002403459892036517 0.9182531951091364 >1k 9.346788468868741E-4 0.8411991001697215 >5k 2.0028832433290162E-4 0.6992394193879415 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6983 0.2637142354326492 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6204 0.23429516205415377 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5289 0.1997400245171533 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3747 0.14150612060233947 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.776517763606605E-5 0.0 18-19 0.0 0.0 0.0 3.776517763606605E-5 0.0 20-21 0.0 0.0 0.0 1.1329553290819814E-4 0.0 22-23 0.0 0.0 0.0 2.2659106581639628E-4 0.0 24-25 0.0 0.0 0.0 4.154169539967265E-4 0.0 26-27 0.0 0.0 0.0 5.098298980868916E-4 0.0 28-29 0.0 0.0 0.0 0.0011896030955360804 0.0 30-31 0.0 0.0 0.0 0.002473619135162326 0.0 32-33 0.0 0.0 0.0 0.004191934717603331 0.0 34-35 0.0 0.0 0.0 0.005721424411864006 7.55303552721321E-5 36-37 0.0 0.0 0.0 0.007874039537119772 7.55303552721321E-5 38-39 0.0 0.0 0.0 0.01193379613299687 7.55303552721321E-5 40-41 0.0 0.0 0.0 0.016408969682870696 7.55303552721321E-5 42-43 0.0 0.0 0.0 0.019845600847752706 7.55303552721321E-5 44-45 0.0 0.0 0.0 0.021998215973008474 7.55303552721321E-5 46-47 0.0 0.0 0.0 0.025076077950347855 7.55303552721321E-5 48-49 0.0 0.0 0.0 0.029362425612041353 7.55303552721321E-5 50-51 0.0 0.0 0.0 0.033818716573097145 7.55303552721321E-5 52-53 0.0 0.0 0.0 0.042259233774757904 7.55303552721321E-5 54-55 1.8882588818033024E-5 0.0 0.0 0.049510147880882587 7.55303552721321E-5 56-57 3.776517763606605E-5 0.0 0.0 0.06525822695512212 7.55303552721321E-5 58-59 3.776517763606605E-5 0.0 0.0 0.09462065256716348 7.55303552721321E-5 60-61 3.776517763606605E-5 0.0 0.0 0.11765741092516377 7.55303552721321E-5 62-63 3.776517763606605E-5 0.0 0.0 0.13191376548277872 7.55303552721321E-5 64-65 3.776517763606605E-5 0.0 0.0 0.1595956406900151 7.55303552721321E-5 66-67 3.776517763606605E-5 0.0 0.0 0.2151104518150322 7.55303552721321E-5 68-69 3.776517763606605E-5 0.0 0.0 0.28845042678427246 7.55303552721321E-5 70-71 3.776517763606605E-5 0.0 0.0 0.3514616256700487 1.1329553290819814E-4 72-73 3.776517763606605E-5 0.0 0.0 0.4257646126690086 1.1329553290819814E-4 74-75 3.776517763606605E-5 0.0 0.0 0.5122657520444178 1.1329553290819814E-4 76-77 3.776517763606605E-5 0.0 0.0 0.5726522710844875 1.1329553290819814E-4 78-79 7.55303552721321E-5 0.0 0.0 0.6504863021924197 1.1329553290819814E-4 80-81 7.55303552721321E-5 0.0 0.0 0.7539440063264226 1.3217812172623117E-4 82-83 7.55303552721321E-5 0.0 0.0 0.8490933713804909 1.510607105442642E-4 84-85 7.55303552721321E-5 0.0 0.0 0.9860865532553205 1.510607105442642E-4 86-87 7.55303552721321E-5 0.0 0.0 1.1911703504079774 1.510607105442642E-4 88-89 7.55303552721321E-5 0.0 0.0 1.453392861324002 1.510607105442642E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11380 0.0 23.798046 1 GTATCAA 14920 0.0 22.4825 1 GTACATG 9160 0.0 22.459555 1 TACATGG 9395 0.0 21.235514 2 GAGTACT 8910 0.0 21.135384 12-13 ACATGGG 9015 0.0 20.916742 3 ACTGCGT 345 1.8189894E-12 20.648676 8 TAGACGC 165 6.3331085E-5 20.148863 4 GTACTTT 9575 0.0 19.56755 14-15 AGAGTAC 13800 0.0 19.530207 10-11 TCAACGC 17530 0.0 19.019175 4 AGTACTT 9515 0.0 19.017826 12-13 ATCAACG 17575 0.0 18.971909 3 TATCAAC 17770 0.0 18.792223 2 CAACGCA 17775 0.0 18.757027 5 AACGCAG 18130 0.0 18.440746 6 GTACCTG 2210 0.0 18.274075 1 CATGGGG 5690 0.0 17.44498 4 TACCTGG 2220 0.0 17.117775 2 ACTTTTT 11100 0.0 16.90061 16-17 >>END_MODULE