FastQCFastQC Report
Thu 26 May 2016
SRR936623_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936623_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256142
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42790.34064620082761343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36080.2872286731914067No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35490.2825317519834541No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27890.22202903811830188No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20480.1630388920997785No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17540.13963389489404862No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC17260.13740484754112195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG53050.026.3285941
GTACCTG10700.026.1959381
TACATGG54900.024.74912
ACATGGG53800.024.3711053
TACCTGG11000.023.3220562
CATGGGA30000.022.6445244
GTATCAA87250.022.1612721
ATGGGAG14200.022.0802525
GAGTACT43650.021.66182712-13
TAGGCGG1100.002875405821.5936345
GGTATCA66800.020.9091591
GTACGTG1604.9833798E-520.78471
GTGTAGG6250.020.5234071
GTACTTT48050.019.8767914-15
GTATTAC2902.4338078E-919.6584361
AGTACTT46450.019.48657612-13
TCAACGC98950.019.396084
CAACGCA98950.019.300065
TATCAAC99850.019.2695982
ATCAACG100550.019.0874423
Produced by FastQC (version 0.11.5)