##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936623_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1256142 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.533828977934025 31.0 31.0 34.0 30.0 34.0 2 31.620877257507512 33.0 31.0 34.0 30.0 34.0 3 31.63753938647064 33.0 31.0 34.0 30.0 34.0 4 35.262854040387154 37.0 35.0 37.0 33.0 37.0 5 35.2703675221432 37.0 35.0 37.0 33.0 37.0 6 35.259465092322365 37.0 35.0 37.0 33.0 37.0 7 35.17567281406083 37.0 35.0 37.0 32.0 37.0 8 35.13844533500193 37.0 35.0 37.0 32.0 37.0 9 36.66080825257017 39.0 37.0 39.0 32.0 39.0 10-11 36.549332400317795 39.0 37.0 39.0 32.0 39.0 12-13 36.453939920805134 39.0 37.0 39.0 32.0 39.0 14-15 37.59036279337845 40.0 37.0 41.0 32.0 41.0 16-17 37.489650453531524 40.0 37.0 41.0 32.0 41.0 18-19 37.45408560497141 40.0 37.0 41.0 32.0 41.0 20-21 37.320630947775015 40.0 37.0 41.0 31.5 41.0 22-23 37.42999477766049 40.0 37.0 41.0 32.0 41.0 24-25 37.465187056877326 40.0 37.0 41.0 32.0 41.0 26-27 37.37432312588864 40.0 37.0 41.0 32.0 41.0 28-29 37.23142208444587 40.0 37.0 41.0 31.0 41.0 30-31 37.030602829934836 39.0 36.0 41.0 31.0 41.0 32-33 36.83807284526749 39.0 36.0 41.0 30.0 41.0 34-35 36.63958055697525 39.0 35.5 41.0 30.0 41.0 36-37 36.433016330956214 39.0 35.0 41.0 30.0 41.0 38-39 36.24450977676091 39.0 35.0 40.0 30.0 41.0 40-41 36.03468477289988 39.0 35.0 40.0 29.5 41.0 42-43 35.80288613866904 38.5 35.0 40.0 28.5 41.0 44-45 35.53853943264376 38.0 35.0 40.0 27.5 41.0 46-47 35.36084057375679 38.0 35.0 40.0 26.5 41.0 48-49 35.191204497580685 38.5 35.0 40.0 26.0 41.0 50-51 34.21373738000958 37.5 33.5 39.5 24.0 40.5 52-53 34.33811981447957 38.0 33.5 39.5 24.0 40.5 54-55 34.77439931154281 38.0 34.0 40.0 25.0 41.0 56-57 34.656354138306014 38.0 34.0 40.0 24.5 41.0 58-59 34.41885750177926 38.0 34.0 40.0 24.0 41.0 60-61 34.125288781045455 38.0 34.0 40.0 23.0 41.0 62-63 33.77269329422947 37.0 33.0 40.0 22.5 41.0 64-65 33.413959170221204 36.5 33.0 39.5 22.0 41.0 66-67 32.9417072273676 36.0 32.0 39.0 20.0 41.0 68-69 32.56111968232891 35.5 32.0 39.0 19.5 40.5 70-71 32.15290349339485 35.0 31.5 38.5 18.0 40.0 72-73 31.762324641640834 35.0 31.0 37.5 17.5 40.0 74-75 31.33358091680718 35.0 31.0 37.0 14.0 39.0 76-77 30.874282923427447 35.0 30.5 36.5 9.5 39.0 78-79 30.381712019819417 34.0 30.0 36.0 7.0 38.5 80-81 29.91867479950515 34.0 29.5 35.5 4.5 37.0 82-83 29.509408172006033 34.0 29.0 35.0 2.0 37.0 84-85 29.151742000506314 34.0 29.0 35.0 2.0 36.5 86-87 28.80292713721856 34.0 29.0 35.0 2.0 36.0 88-89 28.402357774837558 34.0 28.5 35.0 2.0 36.0 90-91 28.003575630780595 34.0 27.0 35.0 2.0 35.0 92-93 27.55168563745182 33.5 26.5 35.0 2.0 35.0 94-95 27.06369900855158 33.0 25.5 35.0 2.0 35.0 96-97 26.48917877118988 33.0 25.0 35.0 2.0 35.0 98-99 25.745700326873873 33.0 23.5 35.0 2.0 35.0 100-101 22.89613037379532 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4942681619720055 1101 2 0.4653015552462634 1101 3 0.45233043839882825 1101 4 0.48041865763774183 1101 5 0.1990129713175861 1101 6 0.41420925975092615 1101 7 0.3923421269843459 1101 8 0.4400354105701254 1101 9 0.5087472942134283 1101 10-11 0.5596878660302096 1101 12-13 0.5639179929605902 1101 14-15 0.5853731013428387 1101 16-17 0.5975274619899551 1101 18-19 0.6587424905850341 1101 20-21 0.5515904851234765 1101 22-23 0.5002624863724563 1101 24-25 0.4312149038374926 1101 26-27 0.47993768560462513 1101 28-29 0.4527744013639392 1101 30-31 0.4624162593900536 1101 32-33 0.3307329505423482 1101 34-35 0.30893156787789877 1101 36-37 0.15857377029607278 1101 38-39 0.11060062678723881 1101 40-41 0.1831108522478715 1101 42-43 0.14466476455429955 1101 44-45 0.028377900975620207 1101 46-47 0.27057358433861367 1101 48-49 0.5394111715557699 1101 50-51 0.36475992954604664 1101 52-53 0.35820812957131665 1101 54-55 0.2537307424549411 1101 56-57 0.1903610980149324 1101 58-59 0.3685131603056817 1101 60-61 0.39782880973043433 1101 62-63 0.2632033102923188 1101 64-65 0.19349159537951977 1101 66-67 0.28482444264448503 1101 68-69 0.15617642886575567 1101 70-71 0.21267691591519622 1101 72-73 0.25822362326371007 1101 74-75 0.31982010322216325 1101 76-77 0.2525945255618183 1101 78-79 0.18003398832806639 1101 80-81 0.14510809563158844 1101 82-83 0.13851728455362533 1101 84-85 0.14421039207318032 1101 86-87 0.10803604695173874 1101 88-89 0.12877206245046224 1101 90-91 0.15612129344278358 1101 92-93 -0.013910863042681854 1101 94-95 0.07646602336481223 1101 96-97 -0.047297643212935725 1101 98-99 0.024316323101622572 1101 100-101 0.3937461030260998 1102 1 0.2304917843342409 1102 2 0.29013403849458896 1102 3 0.35295383407173375 1102 4 0.29487232522672 1102 5 0.4007942605576176 1102 6 0.310965602051823 1102 7 0.3241853113027773 1102 8 0.3501264196710352 1102 9 0.41130421657233285 1102 10-11 0.38535098789795086 1102 12-13 0.4124639586682761 1102 14-15 0.5284785229961102 1102 16-17 0.36724610052047524 1102 18-19 0.37367231690099345 1102 20-21 0.2328613899917329 1102 22-23 0.15119980232628194 1102 24-25 0.3291908125394727 1102 26-27 0.13497818965503683 1102 28-29 -0.07409773029366562 1102 30-31 0.3050171861493922 1102 32-33 0.08324431278968802 1102 34-35 0.2021764479214525 1102 36-37 0.13880512676504964 1102 38-39 0.11428155043515886 1102 40-41 -0.06606406524387154 1102 42-43 0.010123532653331324 1102 44-45 0.07254065058391035 1102 46-47 0.12323940647637954 1102 48-49 0.4939260674898094 1102 50-51 0.5503145961238118 1102 52-53 0.6262960494744121 1102 54-55 0.5219547870816257 1102 56-57 0.46068535266261534 1102 58-59 0.5081937950358153 1102 60-61 0.2997189987493343 1102 62-63 0.19063772039999805 1102 64-65 0.34652912135434377 1102 66-67 0.29773406694025795 1102 68-69 0.3733398118707285 1102 70-71 0.3987927497207977 1102 72-73 0.1620001120236978 1102 74-75 0.08675235200259479 1102 76-77 0.21681316964844655 1102 78-79 0.1636573506642982 1102 80-81 0.19834953088622598 1102 82-83 0.30300873369853676 1102 84-85 -0.09870001007815077 1102 86-87 -0.04013710369666157 1102 88-89 -0.04319513426920807 1102 90-91 -0.2908011765819971 1102 92-93 -0.21997313593664103 1102 94-95 -0.4992748840592647 1102 96-97 -0.2724507140755783 1102 98-99 -0.3144536650083012 1102 100-101 -0.2984203358400066 1103 1 0.18516070205034652 1103 2 0.11475891144237238 1103 3 0.0819454749451225 1103 4 0.14950193890503982 1103 5 0.2751275753874296 1103 6 0.2562149498025903 1103 7 0.373441373918709 1103 8 0.3144771469552481 1103 9 -0.07234313432913808 1103 10-11 0.31450545978503897 1103 12-13 0.02881954280858423 1103 14-15 0.2756993255513862 1103 16-17 0.3264008467701629 1103 18-19 0.3530731089794372 1103 20-21 0.3820332934747981 1103 22-23 0.11041420649046074 1103 24-25 0.23488951518719858 1103 26-27 0.1485175054967911 1103 28-29 0.3140152866804442 1103 30-31 0.22693486588401868 1103 32-33 0.2279447439217961 1103 34-35 0.40305038851493435 1103 36-37 0.47225522371713424 1103 38-39 0.25421923822200654 1103 40-41 0.02437557849309968 1103 42-43 0.1647614729175686 1103 44-45 0.3997170957251015 1103 46-47 0.5631021549898847 1103 48-49 0.4564929593252245 1103 50-51 0.4119355112315546 1103 52-53 0.41610446781230337 1103 54-55 0.5241817500343728 1103 56-57 0.3461132849448276 1103 58-59 0.35391747106080373 1103 60-61 0.4261739978592587 1103 62-63 0.4595601261899347 1103 64-65 0.317726631162806 1103 66-67 0.5968671449249499 1103 68-69 0.424346850228531 1103 70-71 0.29539984141979403 1103 72-73 0.09570629137133935 1103 74-75 0.14173158658197949 1103 76-77 0.41150773069699653 1103 78-79 0.21826109131124483 1103 80-81 0.3653120944282868 1103 82-83 0.209672012306104 1103 84-85 0.20049846829410356 1103 86-87 0.4151845417898521 1103 88-89 0.24652698745973467 1103 90-91 0.3495466194887449 1103 92-93 0.2559635092723198 1103 94-95 0.32281420849600195 1103 96-97 0.38747205604238033 1103 98-99 0.07549598626407317 1103 100-101 0.19529851523150654 1104 1 0.30223365002255775 1104 2 0.38926269408424474 1104 3 0.32283698847176723 1104 4 0.46799535477195064 1104 5 0.5008081100864104 1104 6 0.5435259748915868 1104 7 0.6174497366975871 1104 8 0.5316506256422784 1104 9 0.5789135657882056 1104 10-11 0.6307195617492596 1104 12-13 0.6276369667205941 1104 14-15 0.5730103109310818 1104 16-17 0.4660415834835945 1104 18-19 0.6062993242660681 1104 20-21 0.3771058655322932 1104 22-23 0.31197878605830454 1104 24-25 0.4445913578973304 1104 26-27 0.2317844360538217 1104 28-29 0.3654859868414633 1104 30-31 0.3170839147469735 1104 32-33 0.20305743561017664 1104 34-35 0.4764105101842304 1104 36-37 0.2529539882945855 1104 38-39 0.3435578864247262 1104 40-41 0.09206554282751966 1104 42-43 0.32282335456928735 1104 44-45 0.3981522183375077 1104 46-47 0.33701145629810725 1104 48-49 0.5580787833693392 1104 50-51 0.36178233260125126 1104 52-53 0.5769584097215628 1104 54-55 0.34290945077978563 1104 56-57 0.32937581438761043 1104 58-59 0.33428552333942463 1104 60-61 0.3801537302318039 1104 62-63 0.5840352188615228 1104 64-65 0.6092089529127165 1104 66-67 0.5444366378570251 1104 68-69 0.5267707059884614 1104 70-71 0.6590913769189228 1104 72-73 0.5517330382331416 1104 74-75 0.6094871374459565 1104 76-77 0.6410915242721664 1104 78-79 0.8621204449364761 1104 80-81 0.946324363940608 1104 82-83 0.6903277915647443 1104 84-85 0.6079499848916008 1104 86-87 0.607931670972814 1104 88-89 0.7638038426948448 1104 90-91 0.6213006984612797 1104 92-93 0.1995754739495723 1104 94-95 0.340807154800423 1104 96-97 0.48493820551745515 1104 98-99 0.5869729671129384 1104 100-101 0.39277502221604266 1105 1 0.17624006836145867 1105 2 0.22681239475751624 1105 3 0.21871930280455842 1105 4 0.18070560027859273 1105 5 0.18586388192793635 1105 6 0.19839379096369214 1105 7 0.22724858498919787 1105 8 0.3739791907048158 1105 9 0.33112457645204074 1105 10-11 0.392553244556197 1105 12-13 0.1896266172354828 1105 14-15 0.24214269521617382 1105 16-17 0.036588385886496155 1105 18-19 0.1527279573809679 1105 20-21 0.05864277788983685 1105 22-23 -0.1493410496488039 1105 24-25 -0.022969431509991978 1105 26-27 -0.19920788473876172 1105 28-29 -0.1989459487126055 1105 30-31 -0.2615455729949687 1105 32-33 -0.20734472962922723 1105 34-35 -0.13122016242866863 1105 36-37 -0.18750745503714938 1105 38-39 -0.17494337271656946 1105 40-41 -0.3037989811687041 1105 42-43 -0.3404173193217517 1105 44-45 -0.13836564281496777 1105 46-47 -0.04544329924885204 1105 48-49 0.034206051637248436 1105 50-51 0.0028586652356352715 1105 52-53 0.05725932549209034 1105 54-55 -0.11037727072026371 1105 56-57 -0.11061202600370024 1105 58-59 -0.03918303958136704 1105 60-61 -0.05496733041770341 1105 62-63 0.08611824273511814 1105 64-65 -0.01307266638790594 1105 66-67 0.07660087548065775 1105 68-69 0.05343202979677386 1105 70-71 0.02207469545575691 1105 72-73 -0.22751738407144373 1105 74-75 -0.05900375839248895 1105 76-77 -0.07822308142143442 1105 78-79 0.18168480622884786 1105 80-81 0.3911028137977688 1105 82-83 0.2029083230640012 1105 84-85 0.15916715481176524 1105 86-87 0.02781142211536647 1105 88-89 -0.11030240879488318 1105 90-91 -0.0856905583655454 1105 92-93 -0.25295628093758893 1105 94-95 0.004655118238691358 1105 96-97 0.0619320186256509 1105 98-99 0.022519095901156305 1105 100-101 -0.07724135180246705 1106 1 0.23938198931565324 1106 2 0.3361117307263548 1106 3 0.3039008723151859 1106 4 0.33349269468313736 1106 5 0.1629021289629904 1106 6 0.3569454877711209 1106 7 0.4945699393421279 1106 8 0.4763791752397566 1106 9 0.43482169318427566 1106 10-11 0.45817440849553037 1106 12-13 0.4028579455877761 1106 14-15 0.4479449937112321 1106 16-17 0.36282293290512513 1106 18-19 0.3030222768698465 1106 20-21 0.010050581947488979 1106 22-23 0.45105137633637327 1106 24-25 0.20445302569527968 1106 26-27 0.20658184839223992 1106 28-29 0.11493007947247236 1106 30-31 0.3976624224428349 1106 32-33 0.29315595180013077 1106 34-35 0.3018876387292764 1106 36-37 0.3221379117265002 1106 38-39 0.33336233292528306 1106 40-41 0.4234581931708874 1106 42-43 0.2703985594321523 1106 44-45 0.21743037299805934 1106 46-47 0.30638592778795015 1106 48-49 0.28669834326968413 1106 50-51 0.16412351411296555 1106 52-53 0.16968743711118606 1106 54-55 0.35294346503012264 1106 56-57 0.2686029287126601 1106 58-59 0.33452441941204825 1106 60-61 0.16085149636392515 1106 62-63 0.2628094924088984 1106 64-65 0.4669397056628597 1106 66-67 0.5733677106520716 1106 68-69 0.5535035557152526 1106 70-71 0.2664197983467602 1106 72-73 0.07916835617348639 1106 74-75 0.37920364743977686 1106 76-77 0.3084441007473515 1106 78-79 0.2955183054894235 1106 80-81 0.541352671229216 1106 82-83 0.22598606400486076 1106 84-85 0.43053552295760156 1106 86-87 0.5051386725237421 1106 88-89 0.5034537651484179 1106 90-91 0.3247299947037874 1106 92-93 0.2959345840427403 1106 94-95 0.14907561331403585 1106 96-97 0.02037256028949841 1106 98-99 0.05864065129991047 1106 100-101 -0.06512447458540649 1107 1 0.11817139736596616 1107 2 0.21885789679591383 1107 3 0.2522519798487508 1107 4 0.1892543299373628 1107 5 0.19430052824228738 1107 6 0.2564533433142131 1107 7 0.2980791795628477 1107 8 0.3711820463604667 1107 9 0.3811908765998382 1107 10-11 0.4894593643067964 1107 12-13 0.35645393669580727 1107 14-15 0.059180693369647486 1107 16-17 0.21172374077276146 1107 18-19 0.09574041572965797 1107 20-21 0.15264213958469242 1107 22-23 0.11820949234066092 1107 24-25 0.15218207097674252 1107 26-27 0.24310167806020644 1107 28-29 0.2989322565860846 1107 30-31 0.1398917534183255 1107 32-33 0.14072838670568188 1107 34-35 0.02139365915744662 1107 36-37 0.1022304599086894 1107 38-39 -0.09289190556458493 1107 40-41 0.24774301968997037 1107 42-43 0.13334320986361803 1107 44-45 0.3083375870850702 1107 46-47 0.32323624497087877 1107 48-49 0.3793610116101931 1107 50-51 0.3438919976824337 1107 52-53 0.6359425931585108 1107 54-55 0.678465117300874 1107 56-57 0.44864396855550837 1107 58-59 0.5494632975729274 1107 60-61 0.6621460865285442 1107 62-63 0.7968168829171844 1107 64-65 0.6941050370053787 1107 66-67 0.5431696433617432 1107 68-69 0.770634353531463 1107 70-71 0.4678795488545404 1107 72-73 0.3836256000811069 1107 74-75 0.5114966652445609 1107 76-77 0.5136706331725804 1107 78-79 0.5270447457418364 1107 80-81 0.5250397507689364 1107 82-83 0.5060248099540523 1107 84-85 0.33037161382915414 1107 86-87 0.2623004170507208 1107 88-89 0.22974554007991443 1107 90-91 0.3602674122800167 1107 92-93 0.5471582720398374 1107 94-95 0.3424285351089438 1107 96-97 0.42447946407525805 1107 98-99 0.2556408640064163 1107 100-101 0.16621032127891056 1108 1 0.24433471038273424 1108 2 0.07833726995263746 1108 3 0.16221705875251757 1108 4 0.25709868110047296 1108 5 0.2374273052563325 1108 6 0.280777929465863 1108 7 0.2597400016754605 1108 8 0.2216035031395549 1108 9 0.2662743317137526 1108 10-11 0.23317132786675643 1108 12-13 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-2.666579730732984 2316 62-63 -2.507809972676604 2316 64-65 -2.5255833882907197 2316 66-67 -2.388164633606884 2316 68-69 -2.5109457521023373 2316 70-71 -2.1218016491288125 2316 72-73 -2.0679595321612076 2316 74-75 -2.0762689197426347 2316 76-77 -2.3516081310319663 2316 78-79 -3.324480867454387 2316 80-81 -3.2497288001673645 2316 82-83 -3.0740676946474217 2316 84-85 -2.7328748750472833 2316 86-87 -2.5006981174803293 2316 88-89 -2.820331734647997 2316 90-91 -2.95081713477504 2316 92-93 -3.6028366013196376 2316 94-95 -3.2582349271989948 2316 96-97 -3.6216759306510546 2316 98-99 -4.81974200851279 2316 100-101 -5.871250416157068 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2935.0 3 1110.0 4 1100.0 5 1178.0 6 1398.0 7 1576.0 8 2000.0 9 2586.0 10 3210.0 11 4042.0 12 4970.0 13 6037.0 14 6887.0 15 8259.0 16 9648.0 17 12741.0 18 10437.0 19 7752.0 20 8055.0 21 8554.0 22 9354.0 23 10560.0 24 11904.0 25 13959.0 26 16153.0 27 18844.0 28 22305.0 29 26806.0 30 33193.0 31 40505.0 32 51060.0 33 66703.0 34 89362.0 35 123121.0 36 175133.0 37 232835.0 38 182959.0 39 26905.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.88581173825394 18.05037595661133 14.262704658295089 22.80110764683964 2 15.513920769171364 19.472994050163972 40.56063649630852 24.452448684356142 3 19.95896438006277 23.76793616469026 29.566506526367416 26.70659292887955 4 12.6870645114287 15.85510825954263 37.21957740837969 34.238249820648974 5 13.713434319142126 36.795901108019564 34.39129001380844 15.099374559029874 6 32.396574944786074 35.1135061258981 17.62855306470124 14.861365864614587 7 28.984053436512298 32.19478213793817 20.505147006138195 18.316017419411338 8 26.699346092961186 33.79477893610312 19.66482884707876 19.841046123856938 9 26.209293361747942 16.992940944116867 20.743223656756594 36.054542037378596 10-11 25.30083924393185 25.96320323910886 27.159631554552448 21.57632596240684 12-13 26.39832261409095 23.352725254397026 27.730062229074186 22.518889902437838 14-15 22.959464818386397 24.82779873833212 26.460704667470402 25.75203177581108 16-17 22.29772558238055 28.23783200110846 27.038829616959468 22.42561279955152 18-19 22.076789733977456 26.713969826518806 29.345291037057148 21.86394940244659 20-21 22.806831699295 25.617040459821933 29.43523031517778 22.14089752570529 22-23 22.45308399041556 25.621768248188985 29.46637107089243 22.458776690503022 24-25 22.601417151264037 25.678094659590332 29.553268161305745 22.167220027839885 26-27 22.24040422696449 25.82698710161242 29.730815220766072 22.20179345065702 28-29 21.86365076559816 26.428739744391955 29.576831281813682 22.130778208196208 30-31 22.03247880462841 25.796276843257942 29.61639949956672 22.554844852546925 32-33 21.819990422862848 26.07059138224838 29.612261283089587 22.497156911799184 34-35 22.75805601595998 25.950648891606203 29.143082549584364 22.148212542849453 36-37 22.504188428116464 25.641459407971805 29.434788094793614 22.41956406911812 38-39 22.2113423482377 26.04832893096481 29.4587713809426 22.28155733985489 40-41 22.349969847686033 26.054584644411673 29.431285563310905 22.16415994459139 42-43 22.21971446609076 26.10379085154641 28.882384645208237 22.794110037154596 44-45 22.321858607795576 26.110938180417044 28.862937785551257 22.704265426236127 46-47 22.749718978578684 26.343887425843782 28.495094464594484 22.41129913098305 48-49 22.72251246380273 26.40992725716731 28.3092814281876 22.558278850842363 50-51 22.322757792734052 26.73361165379355 28.47522422470715 22.468406328765248 52-53 22.7147538843243 26.880961128694103 27.88895686790889 22.515328119072706 54-55 22.313715015649883 26.440006384946173 28.20689295542201 23.03938564398193 56-57 22.559009405441856 26.776591201021866 27.957873937862548 22.706525455673734 58-59 23.016264130304116 26.386360071874577 27.892159477484263 22.705216320337048 60-61 22.632559209654264 26.426618401197032 28.647485468014906 22.293336921133804 62-63 22.375514126052384 26.58843206928546 28.55207216731621 22.48398163734594 64-65 22.183620537299365 26.855813153692203 28.286631840233607 22.673934468774828 66-67 22.290540623926375 26.85949574251841 27.858209481072528 22.99175415248269 68-69 22.182101841613072 26.675854357709117 28.255693892999368 22.886349907678436 70-71 22.45640625976919 26.59107643992652 28.008353367849647 22.944163932454643 72-73 22.37844530212764 26.52170537852293 28.288152360630352 22.811696958719075 74-75 22.161445911693765 27.247133077292446 27.82844092439096 22.762980086622832 76-77 22.411390550448782 26.740338002688226 27.80161293404934 23.046658512813654 78-79 22.42769755235008 26.83271073445428 27.850759464432212 22.88883224876343 80-81 22.57027293375706 26.985380170536793 27.71975752322514 22.724589372481006 82-83 22.500453977801282 26.84537993959706 27.95495743759 22.69920864501166 84-85 22.414907123091016 26.87560238913353 27.69455302875583 23.014937459019624 86-87 22.521799817105677 26.930651838716983 27.75295969118966 22.79458865298768 88-89 22.314471067618705 26.877564142120907 27.482268727070775 23.325696063189618 90-91 22.318343715967757 26.703737549300094 27.81877076775749 23.15914796697466 92-93 22.543319231341936 26.670162304286098 27.99842497238924 22.78809349198273 94-95 22.27144866945279 26.621055791182552 27.999573243990582 23.107922295374074 96-97 22.083302313563888 26.614404824513038 28.205828478656475 23.0964643832666 98-99 22.124657862112606 26.829026434768892 27.605634139357694 23.440681563760805 100-101 22.089183645018025 26.560076477265937 27.83848160761586 23.51225827010018 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 86.0 1 81.0 2 138.0 3 207.5 4 177.0 5 161.0 6 287.0 7 573.5 8 958.0 9 1218.0 10 1172.0 11 1073.0 12 1113.5 13 1200.5 14 1336.5 15 1450.5 16 1505.5 17 1614.5 18 1740.0 19 1840.0 20 2067.0 21 2369.5 22 2778.0 23 3407.0 24 4127.5 25 5091.5 26 6407.5 27 8041.5 28 9652.0 29 11459.0 30 13861.5 31 16620.5 32 19648.0 33 21776.0 34 23821.0 35 26101.5 36 28564.0 37 31282.0 38 32852.5 39 33818.5 40 35044.0 41 37212.5 42 39298.0 43 40973.5 44 42699.5 45 44950.5 46 46052.5 47 48883.5 48 56223.0 49 55863.5 50 49028.5 51 45442.0 52 43301.5 53 42530.0 54 41628.0 55 39074.0 56 35997.5 57 32440.0 58 29107.0 59 25633.0 60 21855.5 61 18285.5 62 15095.0 63 11881.0 64 8913.5 65 6888.0 66 5202.5 67 4181.5 68 3083.0 69 1993.5 70 1468.5 71 1088.5 72 805.5 73 629.0 74 499.5 75 405.5 76 314.5 77 199.0 78 104.5 79 62.5 80 55.0 81 33.5 82 17.0 83 13.0 84 11.0 85 7.5 86 5.0 87 3.5 88 3.0 89 4.0 90 4.0 91 3.0 92 2.5 93 1.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011622889768831867 2 0.011224845598666393 3 0.012737413445295198 4 0.017911987657446372 5 0.030888227604840855 6 0.04545664423289724 7 0.005731836050382839 8 0.024360303214127067 9 0.035823975314892745 10-11 0.038849111008150354 12-13 0.030131943681526448 14-15 0.027385438907384672 16-17 0.027703874243517054 18-19 0.02157399402296874 20-21 0.04876041084527068 22-23 0.01174230301988151 24-25 0.002428069438009397 26-27 0.0012737413445295196 28-29 0.0 30-31 0.002428069438009397 32-33 0.002030025267843922 34-35 0.0 36-37 1.9902208508273747E-4 38-39 0.0 40-41 0.001552372263645352 42-43 0.003104744527290704 44-45 0.0015125678466288046 46-47 7.960883403309499E-4 48-49 3.5823975314892744E-4 50-51 0.0028659180251914195 52-53 0.002826113608174872 54-55 0.004975552127068437 56-57 5.970662552482124E-4 58-59 0.005174574212151174 60-61 0.002666895940108682 62-63 4.378485871820224E-4 64-65 6.368706722647598E-4 66-67 0.014050959206841265 68-69 0.03793360941676976 70-71 0.047805104836873544 72-73 0.013891741538775074 74-75 0.02810191841368253 76-77 0.023404997205729923 78-79 0.051904959789577924 80-81 0.07395660681674524 82-83 0.04569547073499652 84-85 0.01635961539380102 86-87 0.01795179207446292 88-89 0.01906631575092625 90-91 0.03498808255754524 92-93 0.02316617070363064 94-95 0.012339369275129724 96-97 0.017712965572363636 98-99 0.04915845501543615 100-101 0.06902085910669335 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1256142.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.07254412178331 #Duplication Level Percentage of deduplicated Percentage of total 1 83.92171261471046 54.609993468963644 2 9.965764105003808 12.969952488602893 3 2.611813115156129 5.098719725215483 4 1.0742261265313158 2.7961050806192573 5 0.612403891550841 1.9925339626646947 6 0.37767469039334883 1.4745751772581226 7 0.25113454653735146 1.1439374702039091 8 0.17752812441712765 0.9241765367192779 9 0.13604289956875237 0.7967391826178677 >10 0.7800498725676035 9.474332888881133 >50 0.059180682400545345 2.605844592160244 >100 0.029896302201666787 3.7025248229970615 >500 0.0013477770748801263 0.5818714187139564 >1k 0.0012252518862546605 1.8286931843824752 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4279 0.34064620082761343 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3608 0.2872286731914067 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3549 0.2825317519834541 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2789 0.22202903811830188 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2048 0.1630388920997785 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1754 0.13963389489404862 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1726 0.13740484754112195 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 7.960883403309499E-5 10-11 0.0 0.0 0.0 0.0 7.960883403309499E-5 12-13 0.0 0.0 0.0 3.9804417016547494E-5 7.960883403309499E-5 14-15 0.0 0.0 0.0 7.960883403309499E-5 7.960883403309499E-5 16-17 0.0 0.0 0.0 7.960883403309499E-5 7.960883403309499E-5 18-19 0.0 0.0 0.0 7.960883403309499E-5 1.5921766806618998E-4 20-21 0.0 0.0 0.0 1.5921766806618998E-4 1.5921766806618998E-4 22-23 0.0 0.0 0.0 3.1843533613237996E-4 1.5921766806618998E-4 24-25 0.0 0.0 0.0 7.164795062978549E-4 1.9902208508273747E-4 26-27 0.0 0.0 0.0 9.553060083971398E-4 2.3882650209928495E-4 28-29 0.0 0.0 0.0 0.0014329590125957098 2.3882650209928495E-4 30-31 0.0 0.0 0.0 0.002348460603976302 2.3882650209928495E-4 32-33 0.0 0.0 0.0 0.004418290288836772 2.3882650209928495E-4 34-35 0.0 0.0 0.0 0.006448315556680694 2.3882650209928495E-4 36-37 0.0 0.0 0.0 0.009752082169054136 2.3882650209928495E-4 38-39 0.0 0.0 0.0 0.01556352705347007 2.3882650209928495E-4 40-41 0.0 0.0 0.0 0.021812820525068027 2.3882650209928495E-4 42-43 0.0 0.0 0.0 0.03156490269412216 2.3882650209928495E-4 44-45 0.0 0.0 0.0 0.041157767195110107 2.7863091911583247E-4 46-47 0.0 0.0 0.0 0.05604461915929887 3.1843533613237996E-4 48-49 0.0 0.0 0.0 0.07463328190602656 3.9804417016547493E-4 50-51 0.0 0.0 0.0 0.08175827255198856 3.9804417016547493E-4 52-53 0.0 0.0 0.0 0.09413744624413482 5.174574212151174E-4 54-55 0.0 0.0 0.0 0.1165075286074345 5.572618382316649E-4 56-57 0.0 0.0 0.0 0.15467996452630356 5.572618382316649E-4 58-59 0.0 0.0 0.0 0.2020870251930116 5.572618382316649E-4 60-61 0.0 0.0 0.0 0.25208137296579525 5.572618382316649E-4 62-63 0.0 0.0 0.0 0.30569792268708473 5.572618382316649E-4 64-65 0.0 0.0 0.0 0.3573242515575468 5.572618382316649E-4 66-67 0.0 0.0 0.0 0.435420517744013 5.572618382316649E-4 68-69 0.0 0.0 0.0 0.5312297495028429 5.572618382316649E-4 70-71 0.0 0.0 0.0 0.584607472722033 5.572618382316649E-4 72-73 0.0 0.0 0.0 0.6583650574536954 6.766750892813075E-4 74-75 0.0 0.0 0.0 0.7353467999636983 7.164795062978549E-4 76-77 0.0 0.0 0.0 0.8193341198686136 7.164795062978549E-4 78-79 0.0 0.0 0.0 0.9207557744267766 7.164795062978549E-4 80-81 0.0 0.0 0.0 1.0683903571411513 7.562839233144023E-4 82-83 0.0 0.0 0.0 1.2294788328071191 7.960883403309499E-4 84-85 0.0 0.0 0.0 1.4665141361406593 7.960883403309499E-4 86-87 0.0 0.0 0.0 1.796492753207838 8.358927573474974E-4 88-89 0.0 0.0 0.0 2.1643253708577532 8.756971743640448E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5305 0.0 26.328594 1 GTACCTG 1070 0.0 26.195938 1 TACATGG 5490 0.0 24.7491 2 ACATGGG 5380 0.0 24.371105 3 TACCTGG 1100 0.0 23.322056 2 CATGGGA 3000 0.0 22.644524 4 GTATCAA 8725 0.0 22.161272 1 ATGGGAG 1420 0.0 22.080252 5 GAGTACT 4365 0.0 21.661827 12-13 TAGGCGG 110 0.0028754058 21.593634 5 GGTATCA 6680 0.0 20.909159 1 GTACGTG 160 4.9833798E-5 20.7847 1 GTGTAGG 625 0.0 20.523407 1 GTACTTT 4805 0.0 19.87679 14-15 GTATTAC 290 2.4338078E-9 19.658436 1 AGTACTT 4645 0.0 19.486576 12-13 TCAACGC 9895 0.0 19.39608 4 CAACGCA 9895 0.0 19.30006 5 TATCAAC 9985 0.0 19.269598 2 ATCAACG 10055 0.0 19.087442 3 >>END_MODULE