FastQCFastQC Report
Thu 26 May 2016
SRR936623_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936623_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256142
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51770.41213493378933275No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46370.36914616341146145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42820.3408850273297127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24630.19607655822351297No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17040.13565345319239386No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16810.13382245000963267No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12790.1018196987283285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG953.49109E-530.0000135
GTACACG1754.944013E-927.1525961
GGACCGT1701.12342605E-725.1460676
GAGTACT53450.023.10663612-13
GTACCTG11100.022.688321
GTAAGGT6100.022.5819784
TAAGGTG7200.022.4305635
GTACATG52850.022.3874051
GTACTTT56650.021.80140514-15
TACATGG54850.021.3935722
ACATGGG52550.020.8809553
TACCTGG11850.020.4463022
AGTACTT55900.019.33217212-13
GGTATCA91500.019.0068171
ACTTTTT65900.018.84826716-17
GTATCAA111300.018.7421231
AGAGTAC87900.018.53601310-11
GGTAAGG7250.018.3455663
GGGCGTA3408.913048E-1018.1603269
TATAGGG2905.2044925E-818.0201172
Produced by FastQC (version 0.11.5)