##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936623_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1256142 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01220244208059 31.0 31.0 34.0 30.0 34.0 2 32.13843578194185 33.0 31.0 34.0 30.0 34.0 3 32.25024002063461 34.0 31.0 34.0 30.0 34.0 4 35.952869978075725 37.0 35.0 37.0 35.0 37.0 5 35.82425155754684 37.0 35.0 37.0 35.0 37.0 6 35.88389927253448 37.0 35.0 37.0 35.0 37.0 7 35.81672135793565 37.0 35.0 37.0 35.0 37.0 8 35.78821423055674 37.0 35.0 37.0 33.0 37.0 9 37.42083219890745 39.0 37.0 39.0 34.0 39.0 10-11 37.32147519945993 39.0 37.0 39.0 34.0 39.0 12-13 37.202882317445 39.0 37.0 39.0 33.0 39.0 14-15 38.32742516371557 40.0 38.0 41.0 33.0 41.0 16-17 38.313594720979 40.0 38.0 41.0 33.5 41.0 18-19 38.44074714482917 40.0 38.0 41.0 34.0 41.0 20-21 38.451558024490865 40.0 38.0 41.0 34.0 41.0 22-23 38.35143518805995 40.0 38.0 41.0 34.0 41.0 24-25 38.23582883145377 40.0 38.0 41.0 33.5 41.0 26-27 38.04308668924374 40.0 37.0 41.0 33.0 41.0 28-29 38.07080807743074 40.0 37.5 41.0 33.0 41.0 30-31 37.96434598954577 40.0 37.0 41.0 33.0 41.0 32-33 37.78395913837767 40.0 37.0 41.0 33.0 41.0 34-35 37.6033597316227 40.0 37.0 41.0 33.0 41.0 36-37 37.430056872551035 40.0 36.0 41.0 32.0 41.0 38-39 37.24057988666887 39.0 36.0 41.0 31.5 41.0 40-41 37.03095072053956 39.0 36.0 41.0 31.0 41.0 42-43 36.78118278029076 39.0 35.5 41.0 31.0 41.0 44-45 36.50178562614737 39.0 35.0 40.0 30.0 41.0 46-47 36.127257109466925 39.0 35.0 40.0 30.0 41.0 48-49 35.72360847738552 39.0 35.0 40.0 28.5 41.0 50-51 35.424910161430795 38.0 35.0 40.0 27.5 41.0 52-53 35.16307272585424 38.0 34.0 40.0 27.0 41.0 54-55 34.83388382842067 38.0 34.0 40.0 26.0 41.0 56-57 34.489088813207424 38.0 34.0 40.0 26.0 41.0 58-59 34.1229960466253 37.0 33.0 40.0 25.0 41.0 60-61 33.74242163704422 37.0 33.0 39.5 24.0 41.0 62-63 33.4198335060845 36.0 32.0 39.0 23.0 40.0 64-65 33.10401252406177 36.0 32.0 39.0 22.5 40.0 66-67 32.66123097547889 35.5 31.5 39.0 22.0 40.0 68-69 32.1965303285775 35.0 31.0 38.0 20.5 40.0 70-71 31.68223815460354 35.0 31.0 37.5 19.5 39.5 72-73 31.12219597784327 34.0 30.0 37.0 18.0 39.0 74-75 30.589780852801674 34.0 29.5 36.0 15.5 39.0 76-77 29.21226740288916 32.5 28.0 35.0 13.0 37.0 78-79 30.002072615994052 34.0 29.0 35.0 12.5 37.0 80-81 29.845125789918654 34.0 29.0 35.0 9.0 37.0 82-83 29.44534813739211 34.0 29.0 35.0 7.0 36.5 84-85 28.9983389616779 34.0 29.0 35.0 4.0 36.0 86-87 28.58460349228033 33.0 29.0 35.0 2.0 36.0 88-89 28.09934545616658 33.0 27.5 35.0 2.0 35.0 90-91 27.532491947566438 33.0 26.5 34.5 2.0 35.0 92-93 26.949029647921968 32.0 25.5 34.0 2.0 35.0 94-95 26.20412620547677 32.0 24.5 34.0 2.0 35.0 96-97 25.38411023594466 32.0 22.0 34.0 2.0 35.0 98-99 24.21319643798233 31.0 10.0 34.0 2.0 35.0 100-101 20.368740556402063 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22792388204446112 1101 2 0.34124559322565773 1101 3 0.2258789705848372 1101 4 0.1668866438234744 1101 5 0.18957167798921404 1101 6 0.22896694096948522 1101 7 0.13883089612215116 1101 8 0.17952298344929574 1101 9 0.2567613581524455 1101 10-11 0.31876647860269713 1101 12-13 0.32703787792056715 1101 14-15 0.36428283991257615 1101 16-17 0.4310984547691987 1101 18-19 0.37319192770324605 1101 20-21 0.32890626967277825 1101 22-23 0.31736030066660703 1101 24-25 0.26286765337529516 1101 26-27 0.2055495074428464 1101 28-29 0.28560813680294217 1101 30-31 0.39066662887777426 1101 32-33 0.32049542811648024 1101 34-35 0.2603483801037001 1101 36-37 0.42044766211250817 1101 38-39 0.3692218390601454 1101 40-41 0.5152406317877549 1101 42-43 0.5663610693674812 1101 44-45 0.8426432806183897 1101 46-47 0.6174751495529378 1101 48-49 0.6809652043281815 1101 50-51 0.5698982683693075 1101 52-53 0.5287776697868765 1101 54-55 0.6906471435676309 1101 56-57 0.5403586264446645 1101 58-59 0.5808956670114469 1101 60-61 0.6621004742576204 1101 62-63 0.5672795809380844 1101 64-65 0.5262826088886499 1101 66-67 0.5004645264114416 1101 68-69 0.433830836124919 1101 70-71 0.21987217825258298 1101 72-73 0.4317268243734169 1101 74-75 0.5381888702566684 1101 76-77 0.4960341578695697 1101 78-79 0.4358201330716156 1101 80-81 0.5805753791792689 1101 82-83 0.6376808906733906 1101 84-85 0.5082021810404491 1101 86-87 0.5961326330932764 1101 88-89 0.3256144844925153 1101 90-91 0.4688331978002722 1101 92-93 0.5811000549742147 1101 94-95 0.6258763015190993 1101 96-97 0.6860367254715207 1101 98-99 0.1605261053302307 1101 100-101 0.2661934232386045 1102 1 0.16277847861522687 1102 2 0.2990302605812829 1102 3 0.19565928194930393 1102 4 0.16593654881397413 1102 5 0.01966579851237782 1102 6 0.13364074168288909 1102 7 0.15521031184150047 1102 8 0.15337592429893476 1102 9 0.02063218968004321 1102 10-11 0.20255207938499353 1102 12-13 0.29511801926803116 1102 14-15 0.2491407701500492 1102 16-17 0.21277384453040327 1102 18-19 0.12009161767224441 1102 20-21 0.16578995804160712 1102 22-23 0.15233546484969196 1102 24-25 0.15727653870827396 1102 26-27 0.06300747546188035 1102 28-29 0.3366243495353345 1102 30-31 0.3703007033963388 1102 32-33 0.32735444735173047 1102 34-35 0.264382116810701 1102 36-37 0.2793613312571992 1102 38-39 0.21250338943740843 1102 40-41 0.29225166622453713 1102 42-43 0.20060504932103385 1102 44-45 0.46813082937326556 1102 46-47 0.22778895871162774 1102 48-49 0.31577035150956334 1102 50-51 0.4063874728453172 1102 52-53 0.4740555309063197 1102 54-55 0.5208384960362125 1102 56-57 0.44164597740198275 1102 58-59 0.24471760476077264 1102 60-61 0.4193623115524545 1102 62-63 0.24113863350999765 1102 64-65 0.25955593622138196 1102 66-67 0.31162897618974483 1102 68-69 0.23017158131055027 1102 70-71 -0.04550158134560078 1102 72-73 -0.11765589167596602 1102 74-75 -0.08500400458637003 1102 76-77 -0.023373338435614954 1102 78-79 0.10423697475578564 1102 80-81 -0.0011689063603981253 1102 82-83 0.20973809639396634 1102 84-85 -0.07003064224187838 1102 86-87 0.13995645992040195 1102 88-89 -0.21940390623544914 1102 90-91 -0.33205244631969677 1102 92-93 -0.09061989479853949 1102 94-95 0.10358796157399652 1102 96-97 -0.15119699790081498 1102 98-99 -0.2975169211946458 1102 100-101 -0.2805834766736055 1103 1 0.15306668057673178 1103 2 0.2748499826583455 1103 3 0.19451270149630062 1103 4 0.04546520662574238 1103 5 0.12006305003716733 1103 6 0.1596818999014218 1103 7 0.16179373817099219 1103 8 0.152660348351219 1103 9 0.08246490633557357 1103 10-11 0.22955781356336757 1103 12-13 0.10677399536402987 1103 14-15 0.2526141687816761 1103 16-17 0.35637618720418374 1103 18-19 0.24955854389868648 1103 20-21 0.23518630927423345 1103 22-23 0.06082437134882923 1103 24-25 0.08195459333705912 1103 26-27 0.19728117066033946 1103 28-29 0.14170918181364556 1103 30-31 0.4428153770101204 1103 32-33 0.3687179592951253 1103 34-35 0.11670772268946905 1103 36-37 0.3234301860613158 1103 38-39 0.04838669983275423 1103 40-41 0.10301755899725151 1103 42-43 0.348381212825835 1103 44-45 0.2503087771282395 1103 46-47 0.444344476622085 1103 48-49 0.590046177803579 1103 50-51 0.3430459213949817 1103 52-53 0.38705610706778515 1103 54-55 0.4220626955833211 1103 56-57 0.4741601788568701 1103 58-59 0.5359507944684765 1103 60-61 0.20867831282600946 1103 62-63 0.4288280154859123 1103 64-65 0.40733704463352893 1103 66-67 0.24895179696907377 1103 68-69 0.35297165583509127 1103 70-71 0.21050185520550002 1103 72-73 0.4074864099965616 1103 74-75 0.5766931897051002 1103 76-77 0.37479315372367594 1103 78-79 0.38932922758539235 1103 80-81 0.6136918801414701 1103 82-83 0.5156726551710484 1103 84-85 0.4582251964175974 1103 86-87 0.36034603567287604 1103 88-89 0.3167924965518232 1103 90-91 0.530034087644804 1103 92-93 0.7583347198847079 1103 94-95 0.8349462989111096 1103 96-97 0.9354985534053064 1103 98-99 0.6036562341475467 1103 100-101 0.7120112852976952 1104 1 0.24841102971149454 1104 2 0.3016845358393567 1104 3 0.2579390450439689 1104 4 0.11984701553002708 1104 5 0.18133814217929256 1104 6 0.13740167268479553 1104 7 0.16164185009298393 1104 8 0.23178973515356915 1104 9 0.2856997583952676 1104 10-11 0.17788227958907754 1104 12-13 0.28283080878524913 1104 14-15 0.3590390675725956 1104 16-17 0.2311009521085765 1104 18-19 0.2566727961626185 1104 20-21 0.2512751945745819 1104 22-23 0.18627399645993847 1104 24-25 0.1586066160957813 1104 26-27 0.17406926395866407 1104 28-29 0.22030431305619658 1104 30-31 0.3152040768018409 1104 32-33 0.45308301749241053 1104 34-35 0.27174425193768315 1104 36-37 0.30193802302287054 1104 38-39 0.33440700716951 1104 40-41 0.3408997692963922 1104 42-43 0.2562827681729871 1104 44-45 0.295267151853011 1104 46-47 0.3709016272414516 1104 48-49 0.5702466237390382 1104 50-51 0.4311448121156545 1104 52-53 0.4619447059316997 1104 54-55 0.19569336995976983 1104 56-57 0.4145244190425501 1104 58-59 0.5136189451599762 1104 60-61 0.45511010118072903 1104 62-63 0.3497411507271906 1104 64-65 0.3305334647076421 1104 66-67 0.4346500287800623 1104 68-69 0.5834221049141988 1104 70-71 0.43042702302693314 1104 72-73 0.32795629016050754 1104 74-75 0.5669857546016814 1104 76-77 0.453115779885092 1104 78-79 0.41159754201158094 1104 80-81 0.45664523296325044 1104 82-83 0.5704934967302755 1104 84-85 0.3953595812973525 1104 86-87 0.3177308406439501 1104 88-89 0.5795874952976696 1104 90-91 0.6122521962775345 1104 92-93 0.3146646803540989 1104 94-95 0.34887814186611266 1104 96-97 0.5775279700558151 1104 98-99 0.4200127932306863 1104 100-101 0.3066855399706512 1105 1 0.13031670292891917 1105 2 0.2211122742557805 1105 3 0.15701567475970535 1105 4 0.08096217600576949 1105 5 0.12793776979127358 1105 6 0.131969229328881 1105 7 0.10952030927711576 1105 8 0.10499265207787545 1105 9 0.16956996839032712 1105 10-11 0.12356354584651541 1105 12-13 0.03133728412230852 1105 14-15 0.1707803442624254 1105 16-17 0.2645184584906417 1105 18-19 0.2592487678086286 1105 20-21 0.08603543956305515 1105 22-23 0.08166336302031851 1105 24-25 0.08266277828838042 1105 26-27 0.18051955637653094 1105 28-29 0.22941210176917792 1105 30-31 0.05221713110900339 1105 32-33 0.13792254174772012 1105 34-35 -0.049245754383932194 1105 36-37 0.11368361775554803 1105 38-39 0.03167299515498456 1105 40-41 -0.09149729719801769 1105 42-43 0.03942616519456976 1105 44-45 0.1307046386829782 1105 46-47 -0.09797927422771835 1105 48-49 -0.11510916572009933 1105 50-51 0.017391845620210233 1105 52-53 -0.11105871741268203 1105 54-55 -0.11781260448432107 1105 56-57 0.019059745814764995 1105 58-59 -0.025619628435009645 1105 60-61 -0.13735387770577034 1105 62-63 -0.0823389714216134 1105 64-65 -0.3038331474941103 1105 66-67 -0.1661717811362351 1105 68-69 -0.16617683526536098 1105 70-71 -0.12085768108349626 1105 72-73 -0.22951616936215657 1105 74-75 -0.22396000041674924 1105 76-77 -0.3473996217101636 1105 78-79 -0.32941557447539793 1105 80-81 -0.0066548571974855975 1105 82-83 -0.13584383568648306 1105 84-85 -0.08167827584797038 1105 86-87 0.07244337989495975 1105 88-89 -0.008710090398210468 1105 90-91 0.005369581408960045 1105 92-93 -0.007000149705007175 1105 94-95 0.09160379414966613 1105 96-97 0.2876948883204449 1105 98-99 0.19888011877311484 1105 100-101 -0.05423859028915956 1106 1 0.09458660694886944 1106 2 0.17650716498543773 1106 3 0.08521718453652483 1106 4 0.12779618754657207 1106 5 0.08007919779657158 1106 6 0.1290289341394839 1106 7 0.11596150820616202 1106 8 0.10760624780178318 1106 9 0.11321527715998059 1106 10-11 0.10233291342207451 1106 12-13 0.07627102599106905 1106 14-15 0.08997345457665062 1106 16-17 0.1875604364722534 1106 18-19 0.19174956522595465 1106 20-21 0.08523028969693058 1106 22-23 0.016898576969808232 1106 24-25 0.05141755071031184 1106 26-27 0.10356250092460328 1106 28-29 0.17992196557995754 1106 30-31 0.04554975562692931 1106 32-33 0.17589797162815302 1106 34-35 0.2274174648623415 1106 36-37 0.3199286036920057 1106 38-39 0.3627152437752059 1106 40-41 0.2977293529725813 1106 42-43 0.30596556727655155 1106 44-45 0.5335521434916473 1106 46-47 0.5251554941346299 1106 48-49 0.5339247510971887 1106 50-51 0.37671196276806285 1106 52-53 0.3518376956591638 1106 54-55 0.22908326731029405 1106 56-57 0.3553987786683521 1106 58-59 0.39744264744238933 1106 60-61 0.3060778198749148 1106 62-63 0.3405995839481548 1106 64-65 0.30954606552747066 1106 66-67 0.21161362177699772 1106 68-69 0.10026977643589419 1106 70-71 -0.07444702565477712 1106 72-73 0.030188641239142555 1106 74-75 0.09029878808884106 1106 76-77 -0.10252387382936234 1106 78-79 -0.2411139284127053 1106 80-81 0.011291382375677728 1106 82-83 -0.01087355760531139 1106 84-85 0.15853185570172457 1106 86-87 0.17651842411537544 1106 88-89 -0.20555715058630497 1106 90-91 -0.15419314392184802 1106 92-93 -0.19515584423134058 1106 94-95 -0.2600021560334973 1106 96-97 -0.2246037033922157 1106 98-99 -0.1817711537116331 1106 100-101 -0.3937933897749417 1107 1 0.03787529972143844 1107 2 0.08967536578037993 1107 3 0.031079208918527 1107 4 0.004604922693616231 1107 5 -0.0911528733391549 1107 6 -0.008389893305491114 1107 7 -0.08203696531187887 1107 8 -0.039713024658560414 1107 9 -0.021091779273973543 1107 10-11 -0.022001753150057368 1107 12-13 -0.060136402887799534 1107 14-15 -0.053644516203320336 1107 16-17 0.04494927647108682 1107 18-19 0.06421595968390648 1107 20-21 0.07680148172919132 1107 22-23 -0.040212076289400045 1107 24-25 -0.02095333435727298 1107 26-27 0.02770531457286296 1107 28-29 -0.10582044267471957 1107 30-31 0.042772684553767704 1107 32-33 -0.05098291690111978 1107 34-35 -0.1288577940348361 1107 36-37 0.0800284089195813 1107 38-39 0.04197573416796985 1107 40-41 0.04110166859566533 1107 42-43 -0.027268363998395273 1107 44-45 0.008108066533594638 1107 46-47 0.11922285953519918 1107 48-49 0.02907712819662578 1107 50-51 0.09966216025145513 1107 52-53 0.04112291839624049 1107 54-55 0.00599522767181071 1107 56-57 0.15520567452976053 1107 58-59 0.12631922345159552 1107 60-61 0.03745244607451781 1107 62-63 -0.035595847449833684 1107 64-65 0.11881756660977061 1107 66-67 0.18563902977727054 1107 68-69 0.15641365778737537 1107 70-71 0.03262317166178619 1107 72-73 -0.05906133894995591 1107 74-75 -0.2534174030467149 1107 76-77 -0.2607510276912386 1107 78-79 0.02773249332172867 1107 80-81 0.09531628989005725 1107 82-83 0.14418823154832694 1107 84-85 -0.08386618072530538 1107 86-87 0.15435711751355186 1107 88-89 0.09526468894958029 1107 90-91 -0.0889208170378275 1107 92-93 -0.2568795176309351 1107 94-95 0.111689319440611 1107 96-97 0.45244505644545896 1107 98-99 0.4202080594411335 1107 100-101 0.5102315594465914 1108 1 0.01811028826538319 1108 2 0.16787814941489643 1108 3 -0.03574108863735859 1108 4 0.06146571629174957 1108 5 0.04471404447426153 1108 6 -0.022823976880331998 1108 7 0.07418353202813677 1108 8 0.05814692721411774 1108 9 0.03146259701294696 1108 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sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 11.0 5 40.0 6 93.0 7 189.0 8 437.0 9 832.0 10 1360.0 11 2171.0 12 3178.0 13 4514.0 14 5876.0 15 7629.0 16 9828.0 17 12739.0 18 11610.0 19 8131.0 20 7689.0 21 8140.0 22 8629.0 23 9448.0 24 11321.0 25 13572.0 26 16260.0 27 20123.0 28 24795.0 29 30648.0 30 37756.0 31 46985.0 32 59202.0 33 76303.0 34 102138.0 35 140563.0 36 199774.0 37 244914.0 38 124538.0 39 4698.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.559055920560255 19.26033203927401 14.57409100028981 22.606521039875922 2 15.934414587133395 20.555393225059333 39.189147228806114 24.321044959001156 3 20.418477196479838 24.01467106342122 29.52539571587115 26.041456024227795 4 13.291432483640358 16.72647953253618 36.50301717961374 33.47907080420972 5 13.788705550416132 37.48015661348029 33.76018839474367 14.970949441359908 6 32.46344760385371 36.03454068091028 16.699306288620235 14.80270542661578 7 29.165917012038523 32.621042022586764 19.98457170970613 18.228469255668582 8 26.99282472440907 34.51719144444117 18.87074393429285 19.619239896856904 9 26.372416494313537 17.11661579662172 20.068670580236947 36.442297128827796 10-11 25.55606770572117 26.527653720678074 26.44298972568388 21.473288847916873 12-13 26.635364473124856 23.76486893997653 27.19557183821574 22.40419474868287 14-15 22.95000139316236 25.42318301774889 25.973004493943723 25.65381109514503 16-17 22.296349754218138 28.741172532140325 26.609563698880695 22.352914014760845 18-19 22.10421817264133 27.226282108182748 29.061171882245752 21.608327836930176 20-21 22.915237020136324 25.890547057727737 29.344228102326337 21.84998781980961 22-23 22.483954788407008 26.10632748949558 29.152887660432214 22.2568300616652 24-25 22.785474207451728 26.241623133763962 29.17215305961606 21.800749599168245 26-27 22.48149492652901 26.24683355862633 29.34628409845384 21.92538741639082 28-29 21.94043348602308 26.999773910911344 29.2422751567896 21.81751744627598 30-31 22.212841603138514 26.305909551195093 29.163243470452244 22.31800537521415 32-33 22.10900154167478 26.556611866158907 29.170134738315333 22.164251853850985 34-35 22.774406978887164 26.36969138979478 29.021324598579053 21.83457703273901 36-37 22.78284004731979 26.06274489823538 29.08069521245345 22.07371984199138 38-39 22.346652697263746 26.469285160123352 29.215888975091925 21.96817316752098 40-41 22.46180962169056 26.53675025335532 29.006894926604577 21.99454519834955 42-43 22.349048747983893 26.748101715262862 28.41595721475575 22.48689232199749 44-45 22.57639748585895 26.505479283016015 28.422810376603678 22.495312854521355 46-47 23.00894325641528 27.07552171649383 27.824003974072998 22.091531053017892 48-49 23.094109625192704 27.007412873952486 27.613642108212915 22.284835392641895 50-51 22.643443357059777 27.25971103148338 27.862796875783662 22.234048735673177 52-53 23.138926382602527 27.47193477778888 27.27092159172192 22.118217247886673 54-55 22.580661853505067 27.204473390285628 27.506032373805766 22.70883238240354 56-57 22.89491880866025 27.471219480348356 27.343633373659298 22.290228337332092 58-59 23.3403867939474 27.00774845735403 27.153196228707266 22.49866851999131 60-61 22.748228460701323 27.12674277989764 28.074031673952383 22.05099708544866 62-63 22.626627058539277 27.28486764407934 27.925162770536232 22.16334252684515 64-65 22.36493387904056 27.593628568266876 27.624995322850683 22.416442229841884 66-67 22.55585585944186 27.41409163823952 27.29109530424676 22.738957198071862 68-69 22.517050561372866 27.29526787711654 27.507588863139343 22.68009269837125 70-71 22.726999512374736 27.389373749838285 27.392797078229027 22.490829659557953 72-73 22.765241544461116 27.140798072150268 27.561062307616062 22.53289807577255 74-75 22.519642474716957 27.931125351192083 27.021469191030185 22.52776298306078 76-77 22.7165001102592 27.437424918260916 27.13132627090646 22.714748700573427 78-79 22.75727221630435 27.541155752570294 27.091861837045673 22.609710194079685 80-81 22.933109574449453 27.703496657150545 26.981587658445015 22.381806109954987 82-83 22.787903877462472 27.681921495416383 27.104382197348155 22.42579242977299 84-85 22.69818599844919 27.767415163225113 27.016251657885643 22.518147180440053 86-87 22.686653902645816 27.710437174348296 26.88847138245491 22.714437540550975 88-89 22.419407676725537 27.84133541221928 26.6029997894312 23.13625712162398 90-91 22.544226298525523 27.694500175153657 26.80698863093532 22.954284895385495 92-93 22.72490794068558 27.559838056809085 27.01709510918348 22.698158893321853 94-95 22.41848594058113 27.57020952399149 27.020546391186283 22.9907581442411 96-97 22.332370196439324 27.45736238480072 27.276530414440764 22.93373700431919 98-99 22.22310677045602 27.783580414191476 26.527822668600642 23.46549014675186 100-101 21.890334969063368 27.846671804196426 26.692240207114633 23.570753019625577 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 206.0 1 154.5 2 174.0 3 244.5 4 203.0 5 172.5 6 278.5 7 583.5 8 945.5 9 1189.5 10 1207.0 11 1124.0 12 1162.5 13 1283.0 14 1405.0 15 1500.5 16 1635.5 17 1830.0 18 1992.0 19 2079.5 20 2253.0 21 2583.5 22 2955.0 23 3504.0 24 4204.5 25 5082.0 26 6424.5 27 8036.5 28 9608.5 29 11660.0 30 14125.5 31 16576.0 32 19392.0 33 21623.0 34 23911.0 35 26103.5 36 28659.0 37 31411.0 38 32820.0 39 33567.5 40 34604.0 41 36929.5 42 39011.0 43 40927.0 44 42985.5 45 44557.0 46 45303.5 47 48418.0 48 56348.5 49 56109.0 50 48920.5 51 45352.0 52 42927.0 53 41910.0 54 41195.5 55 38836.0 56 35429.5 57 32141.0 58 29531.0 59 26352.5 60 22412.5 61 18576.0 62 15339.0 63 11959.5 64 8684.0 65 6719.0 66 5251.0 67 4362.0 68 3260.5 69 2063.5 70 1473.5 71 1076.5 72 853.5 73 711.0 74 569.5 75 433.5 76 310.5 77 199.5 78 119.0 79 76.0 80 44.0 81 30.5 82 22.5 83 14.0 84 12.5 85 6.5 86 2.0 87 1.5 88 1.0 89 0.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011622889768831867 2 0.010428757258335444 3 0.005094965378118078 4 1.5921766806618995E-4 5 0.004458094705853319 6 0.0 7 6.368706722647598E-4 8 0.0018310031827611848 9 0.0 10-11 0.0 12-13 0.0 14-15 5.572618382316648E-4 16-17 0.003861028450605107 18-19 0.0013931545955791621 20-21 3.184353361323799E-4 22-23 3.184353361323799E-4 24-25 3.184353361323799E-4 26-27 0.0 28-29 0.0 30-31 4.776530041985699E-4 32-33 0.003224157778340347 34-35 8.358927573474973E-4 36-37 6.368706722647598E-4 38-39 0.005055160961101532 40-41 7.960883403309498E-5 42-43 6.368706722647598E-4 44-45 0.003741615199555464 46-47 0.0 48-49 0.0013135457615460673 50-51 0.0015125678466288046 52-53 4.378485871820224E-4 54-55 2.3882650209928495E-4 56-57 0.01015012633921961 58-59 0.0016717855146949946 60-61 0.0024678738550259443 62-63 1.9902208508273747E-4 64-65 0.0027863091911583242 66-67 5.572618382316648E-4 68-69 0.0019902208508273747 70-71 0.004338681454803677 72-73 0.002189242935910112 74-75 0.005015356544084984 76-77 8.756971743640448E-4 78-79 0.004776530041985699 80-81 0.0022290473529266593 82-83 0.0029455268592245144 84-85 0.0019106120167942796 86-87 5.572618382316648E-4 88-89 0.0016319810976784474 90-91 0.008120101071375688 92-93 0.0017911987657446373 94-95 7.164795062978549E-4 96-97 2.786309191158324E-4 98-99 6.368706722647598E-4 100-101 0.0020698296848604696 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1256142.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.66226273936844 #Duplication Level Percentage of deduplicated Percentage of total 1 82.78797327953112 51.876817333017925 2 10.439496706159733 13.08324970936305 3 2.890434103340101 5.433634236429846 4 1.2235151810576415 3.066729189641595 5 0.6560622992457752 2.055517408436646 6 0.41323820507099435 1.55366645880622 7 0.2795196010880184 1.2260731478926603 8 0.1883017732302138 0.9439532150752504 9 0.14672420375569784 0.8274663545367749 >10 0.872684839377481 10.096064993607548 >50 0.06477321997648879 2.8117806513585206 >100 0.033853241263401 4.026609011003831 >500 0.001648278138574956 0.688512618662793 >1k 0.001648278138574956 1.8986136681496382 >5k 1.267906260442274E-4 0.41131200401768747 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5177 0.41213493378933275 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4637 0.36914616341146145 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4282 0.3408850273297127 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2463 0.19607655822351297 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1704 0.13565345319239386 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1681 0.13382245000963267 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1279 0.1018196987283285 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 3.9804417016547494E-5 16-17 0.0 0.0 0.0 0.0 7.960883403309499E-5 18-19 0.0 0.0 0.0 0.0 7.960883403309499E-5 20-21 0.0 0.0 0.0 1.1941325104964248E-4 1.1941325104964249E-4 22-23 0.0 0.0 0.0 3.5823975314892744E-4 1.9902208508273747E-4 24-25 0.0 0.0 0.0 8.358927573474974E-4 2.3882650209928495E-4 26-27 0.0 0.0 0.0 0.0010747192594467822 2.3882650209928495E-4 28-29 0.0 0.0 0.0 0.0013931545955791621 2.3882650209928495E-4 30-31 0.0 0.0 0.0 0.0022290473529266597 2.3882650209928495E-4 32-33 0.0 0.0 0.0 0.004179463786737487 2.3882650209928495E-4 34-35 0.0 0.0 0.0 0.006249293471597956 2.3882650209928495E-4 36-37 0.0 0.0 0.0 0.009592864500987945 2.3882650209928495E-4 38-39 0.0 0.0 0.0 0.015364504968387331 2.3882650209928495E-4 40-41 0.0 0.0 0.0 0.021773016108051477 2.3882650209928495E-4 42-43 0.0 0.0 0.0 0.03148529386008907 2.3882650209928495E-4 44-45 0.0 0.0 0.0 0.04087913627599428 2.3882650209928495E-4 46-47 0.0 0.0 0.0 0.05588540149123268 2.3882650209928495E-4 48-49 0.0 0.0 0.0 0.07411582448481144 2.3882650209928495E-4 50-51 0.0 0.0 0.0 0.08100198862867415 2.3882650209928495E-4 52-53 0.0 0.0 0.0 0.09326174906977078 2.3882650209928495E-4 54-55 0.0 0.0 0.0 0.11547261376500428 2.3882650209928495E-4 56-57 0.0 0.0 0.0 0.1536848541008899 2.3882650209928495E-4 58-59 0.0 0.0 0.0 0.20113171918461448 2.3882650209928495E-4 60-61 0.0 0.0 0.0 0.2516037199615967 2.3882650209928495E-4 62-63 0.0 0.0 0.0 0.3056183138530516 2.3882650209928495E-4 64-65 0.0 0.0 0.0 0.3570058162214145 3.1843533613237996E-4 66-67 0.0 0.0 0.0 0.435420517744013 3.1843533613237996E-4 68-69 0.0 0.0 0.0 0.5310307274177601 3.1843533613237996E-4 70-71 0.0 0.0 0.0 0.5849259080581655 3.1843533613237996E-4 72-73 0.0 0.0 0.0 0.6596387987982251 3.1843533613237996E-4 74-75 0.0 0.0 0.0 0.7366603457252445 3.1843533613237996E-4 76-77 0.0 0.0 0.0 0.8203690347110437 3.1843533613237996E-4 78-79 0.0 0.0 0.0 0.9221887334393724 3.1843533613237996E-4 80-81 0.0 0.0 0.0 1.0695844896516475 3.1843533613237996E-4 82-83 0.0 0.0 0.0 1.2292798107220362 3.9804417016547493E-4 84-85 0.0 0.0 0.0 1.465917069885411 3.9804417016547493E-4 86-87 0.0 0.0 0.0 1.7939850749357955 4.378485871820224E-4 88-89 0.0 0.0 0.0 2.155886834450245 4.776530041985699E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 95 3.49109E-5 30.000013 5 GTACACG 175 4.944013E-9 27.152596 1 GGACCGT 170 1.12342605E-7 25.146067 6 GAGTACT 5345 0.0 23.106636 12-13 GTACCTG 1110 0.0 22.68832 1 GTAAGGT 610 0.0 22.581978 4 TAAGGTG 720 0.0 22.430563 5 GTACATG 5285 0.0 22.387405 1 GTACTTT 5665 0.0 21.801405 14-15 TACATGG 5485 0.0 21.393572 2 ACATGGG 5255 0.0 20.880955 3 TACCTGG 1185 0.0 20.446302 2 AGTACTT 5590 0.0 19.332172 12-13 GGTATCA 9150 0.0 19.006817 1 ACTTTTT 6590 0.0 18.848267 16-17 GTATCAA 11130 0.0 18.742123 1 AGAGTAC 8790 0.0 18.536013 10-11 GGTAAGG 725 0.0 18.345566 3 GGGCGTA 340 8.913048E-10 18.160326 9 TATAGGG 290 5.2044925E-8 18.020117 2 >>END_MODULE