FastQCFastQC Report
Thu 26 May 2016
SRR936622_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936622_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436474
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50110.34884028530972366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40950.28507303299607234No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39750.2767192444833669No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30640.21330006669107832No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23560.16401271446611634No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC18300.1273952748187576No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18270.12718643010593997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG12750.028.3299562
GTACCTG13700.026.7142451
GTACATG60800.024.9378991
TACATGG62700.023.6499122
ACATGGG59550.023.2248923
GTATCAA105350.022.197461
GAGTACT47900.021.2308712-13
CATGGGG29650.020.3573634
AGAGTAC81150.020.1749710-11
AGTACTT50950.020.00656912-13
TATAACG1200.004748319.803012
GTACTTT52400.019.49490214-15
ATCAACG119600.019.2334253
TATCAAC119650.019.225392
TCAACGC119450.019.217794
CAACGCA120400.019.066825
ATAACGC1250.0060119319.010893
AACGCAG122450.018.747616
AGTACAT46500.018.7042642
GAGTACA50150.018.6710171
Produced by FastQC (version 0.11.5)