##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936622_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1436474 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.372977861068144 31.0 31.0 34.0 30.0 34.0 2 31.429881083820522 31.0 31.0 34.0 28.0 34.0 3 31.46917243194099 31.0 31.0 34.0 28.0 34.0 4 35.11072041679836 37.0 35.0 37.0 32.0 37.0 5 35.03985870959029 37.0 35.0 37.0 32.0 37.0 6 35.14685333671198 37.0 35.0 37.0 32.0 37.0 7 34.9751474791747 37.0 35.0 37.0 32.0 37.0 8 34.96088199299117 37.0 35.0 37.0 32.0 37.0 9 36.471888805505706 39.0 37.0 39.0 32.0 39.0 10-11 36.34560910952791 39.0 37.0 39.0 32.0 39.0 12-13 36.26022294869242 39.0 35.0 39.0 32.0 39.0 14-15 37.351396196520085 40.0 37.0 41.0 32.0 41.0 16-17 37.287041742488896 40.0 37.0 41.0 32.0 41.0 18-19 37.241795187382436 39.5 37.0 41.0 31.0 41.0 20-21 37.13593667549848 39.0 36.0 41.0 31.0 41.0 22-23 37.038617475847104 39.0 36.0 41.0 31.0 41.0 24-25 36.88873937154449 39.0 36.0 41.0 30.5 41.0 26-27 36.69290986122965 39.0 36.0 40.5 30.0 41.0 28-29 36.795464449756835 39.0 36.0 41.0 30.0 41.0 30-31 36.74392366308057 39.0 36.0 41.0 30.0 41.0 32-33 36.5917799417184 39.0 35.5 41.0 30.0 41.0 34-35 36.41249824222366 39.0 35.0 40.5 30.0 41.0 36-37 36.174653700658695 39.0 35.0 40.0 29.5 41.0 38-39 35.998742058679795 39.0 35.0 40.0 29.0 41.0 40-41 35.85736671878502 38.0 35.0 40.0 28.5 41.0 42-43 35.61008344042426 38.0 35.0 40.0 27.0 41.0 44-45 35.345702045425114 38.0 34.5 40.0 26.5 41.0 46-47 35.001956526884584 38.0 34.0 40.0 25.5 41.0 48-49 34.76105728332013 38.0 34.0 40.0 24.0 41.0 50-51 34.06352986549008 37.5 33.5 39.5 23.5 40.5 52-53 34.249778276529895 38.0 33.5 39.5 24.0 40.5 54-55 34.61233374220487 38.0 34.0 40.0 24.0 41.0 56-57 34.52747804693994 38.0 34.0 40.0 24.0 41.0 58-59 34.29350200560539 38.0 34.0 40.0 23.0 41.0 60-61 33.97660347489756 37.5 33.0 40.0 23.0 41.0 62-63 33.66225702658036 37.0 33.0 40.0 22.0 41.0 64-65 33.35445646771191 36.5 33.0 39.5 22.0 41.0 66-67 32.97931601964254 36.0 32.0 39.0 21.0 41.0 68-69 32.59098528758613 35.5 32.0 39.0 20.0 40.5 70-71 32.18960802632 35.0 31.5 38.5 18.5 40.0 72-73 31.776635706598242 35.0 31.0 37.5 18.0 40.0 74-75 31.351946502338365 35.0 31.0 37.0 15.5 39.0 76-77 30.942917170794598 35.0 31.0 36.5 12.0 39.0 78-79 30.495848515183706 34.5 30.0 36.0 7.5 38.5 80-81 30.07153175066169 34.0 30.0 35.5 7.0 37.0 82-83 29.674681894694928 34.0 29.0 35.0 4.0 37.0 84-85 29.29426498495622 34.0 29.0 35.0 2.0 36.5 86-87 28.965822214672873 34.0 29.0 35.0 2.0 36.0 88-89 28.587605483983697 34.0 29.0 35.0 2.0 36.0 90-91 28.244875646896496 34.0 28.5 35.0 2.0 35.0 92-93 27.878152336902723 34.0 27.0 35.0 2.0 35.0 94-95 27.45958123850484 34.0 27.0 35.0 2.0 35.0 96-97 26.88763353879012 33.0 25.5 35.0 2.0 35.0 98-99 26.23625871404564 33.0 25.0 35.0 2.0 35.0 100-101 24.184627079919302 31.0 12.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24156350795130166 1101 2 0.2669531684219777 1101 3 0.3620879360012772 1101 4 0.2792712185595576 1101 5 0.41736381395490696 1101 6 0.3493530848662232 1101 7 0.1798490237918955 1101 8 0.2926170128907799 1101 9 0.5189054765906604 1101 10-11 0.4975108843584124 1101 12-13 0.5173355976315506 1101 14-15 0.5975069440273586 1101 16-17 0.7674505467711938 1101 18-19 0.7752331011830549 1101 20-21 0.6371023027494758 1101 22-23 0.552383026180614 1101 24-25 0.5477312440959281 1101 26-27 0.5625505691064916 1101 28-29 0.526063664074961 1101 30-31 0.44074127193659507 1101 32-33 0.3695110592612849 1101 34-35 0.43226209840030805 1101 36-37 0.37050951768096496 1101 38-39 0.3771062359988875 1101 40-41 0.3625619222283021 1101 42-43 0.11378067271006387 1101 44-45 0.06494786769845717 1101 46-47 0.3036048568257783 1101 48-49 0.24548687721794948 1101 50-51 0.5314331979411762 1101 52-53 0.548117791184147 1101 54-55 0.5599847648901815 1101 56-57 0.5242261944206845 1101 58-59 0.3768942691166117 1101 60-61 0.3230744262590335 1101 62-63 0.35404999790919334 1101 64-65 0.4057551101406247 1101 66-67 0.27027224890755264 1101 68-69 0.31748505611941624 1101 70-71 0.43528126417606927 1101 72-73 0.39529360701632044 1101 74-75 0.47490588872959094 1101 76-77 0.34972623108879475 1101 78-79 0.32628259141635496 1101 80-81 0.3663653095815391 1101 82-83 0.540453067783897 1101 84-85 0.500984395095923 1101 86-87 0.2705829170432992 1101 88-89 0.30926772443938333 1101 90-91 0.3519900894116361 1101 92-93 0.15585656998455732 1101 94-95 0.05271096347813753 1101 96-97 -0.18904679521173406 1101 98-99 -0.35734495346034123 1101 100-101 -0.026948650490961512 1102 1 0.15097833538793992 1102 2 0.13071101732755608 1102 3 0.19955025580119923 1102 4 0.26216911294284984 1102 5 0.14892949530828758 1102 6 0.19600966712420842 1102 7 0.017112507913147113 1102 8 0.07027397911696198 1102 9 0.4004939359553319 1102 10-11 0.3589204587298056 1102 12-13 0.5008930615621736 1102 14-15 0.25393259444515337 1102 16-17 0.13121608622692804 1102 18-19 0.18977976282709363 1102 20-21 0.031021230927287036 1102 22-23 -0.06069043367485705 1102 24-25 0.046653588276292624 1102 26-27 0.0041201460730988515 1102 28-29 -0.088404162438529 1102 30-31 -0.06237521864444062 1102 32-33 -0.011665466271196578 1102 34-35 -0.0896449231279206 1102 36-37 -0.24997791218471122 1102 38-39 -0.28963677685663924 1102 40-41 -0.4080060346889596 1102 42-43 -0.15122226228353242 1102 44-45 -0.17938174984069377 1102 46-47 -0.047308794516006 1102 48-49 0.10197044446985615 1102 50-51 0.03937306175411237 1102 52-53 -0.2040940809558478 1102 54-55 -0.32811828960058165 1102 56-57 -0.11251696105958331 1102 58-59 -0.11955255688147304 1102 60-61 -0.5721341372664455 1102 62-63 -0.23409223643893284 1102 64-65 -0.15995882320663668 1102 66-67 -0.2866678853331024 1102 68-69 -0.4129614404434676 1102 70-71 -0.14406200199218944 1102 72-73 -0.2657700044604425 1102 74-75 -0.39375978963146707 1102 76-77 -0.2501842229417086 1102 78-79 -0.24860435050489116 1102 80-81 -0.149564336706284 1102 82-83 -0.30895155778838657 1102 84-85 -0.24795535769344212 1102 86-87 -0.18585640857095953 1102 88-89 -0.4153168949636772 1102 90-91 -0.7685876694613754 1102 92-93 -0.7434077100387242 1102 94-95 -0.5502579524367412 1102 96-97 -0.7410775618868755 1102 98-99 -0.821868445574168 1102 100-101 -0.3914155681212499 1103 1 0.24127301319601457 1103 2 0.32572823178258403 1103 3 0.2714672426928253 1103 4 0.0873376313565899 1103 5 0.31970659719655004 1103 6 0.1366271931541263 1103 7 0.20683513216182092 1103 8 0.07055114519861405 1103 9 0.2863322427204551 1103 10-11 0.39325559187148684 1103 12-13 0.30319738877094693 1103 14-15 0.3310952007484005 1103 16-17 0.387465606930661 1103 18-19 0.30401785624614064 1103 20-21 0.18508320756089347 1103 22-23 0.15102194433257665 1103 24-25 0.24092819868561577 1103 26-27 0.40698592846986514 1103 28-29 0.4503083278714328 1103 30-31 0.2585678813131338 1103 32-33 0.15484198547533623 1103 34-35 0.04416224453056117 1103 36-37 0.24145693784546296 1103 38-39 0.19579692487301514 1103 40-41 0.17269668616286538 1103 42-43 0.3560053100259353 1103 44-45 0.3364583635302836 1103 46-47 0.3956640687704649 1103 48-49 0.7020487977414263 1103 50-51 0.11521288216197689 1103 52-53 0.4760265336807592 1103 54-55 0.582060799898791 1103 56-57 0.6243016376174282 1103 58-59 0.5304073617437126 1103 60-61 0.6900333819132953 1103 62-63 0.49684369166676845 1103 64-65 0.5305196171496647 1103 66-67 0.48836602801932827 1103 68-69 0.6111053356152354 1103 70-71 0.6718899543551586 1103 72-73 0.6597561874470586 1103 74-75 0.4294801985695891 1103 76-77 0.2124368433463637 1103 78-79 0.4182713156630662 1103 80-81 0.5763850432889868 1103 82-83 0.574037251882384 1103 84-85 0.5990212486276114 1103 86-87 0.49013529310527915 1103 88-89 0.47531552335777505 1103 90-91 0.564253610304899 1103 92-93 0.5699070038612568 1103 94-95 0.5080069534258804 1103 96-97 0.5651549123847914 1103 98-99 0.5005212315546324 1103 100-101 0.6509664287785064 1104 1 0.1144018791412087 1104 2 0.1452239005975109 1104 3 0.11678632504955289 1104 4 0.1349239912739364 1104 5 0.08063404419402076 1104 6 0.05147495502848187 1104 7 0.16774443080949197 1104 8 0.12363175220966127 1104 9 0.3063310006739215 1104 10-11 0.26536881345680996 1104 12-13 0.15012419786297215 1104 14-15 0.10458600869966261 1104 16-17 -0.11058374693381978 1104 18-19 -0.07213684004634757 1104 20-21 -0.10001862518126359 1104 22-23 -0.061549495953023836 1104 24-25 0.16476828700356805 1104 26-27 -0.019496221934737434 1104 28-29 -0.059638897209715935 1104 30-31 -0.11946556109116813 1104 32-33 -0.11278467463139918 1104 34-35 -0.17869270794910364 1104 36-37 -0.0992219551413811 1104 38-39 -6.841342188508293E-4 1104 40-41 -0.21473892789348525 1104 42-43 -0.17680793139595607 1104 44-45 -0.19207660038102858 1104 46-47 -0.017043574578885057 1104 48-49 -0.12651185120979846 1104 50-51 -0.3091698478442382 1104 52-53 -0.1656353547243654 1104 54-55 0.07903105047263637 1104 56-57 0.2402701957521387 1104 58-59 0.08000240031046246 1104 60-61 -0.12906329452694365 1104 62-63 -0.16133259211795092 1104 64-65 -0.06169299227553893 1104 66-67 -0.21995275950208537 1104 68-69 0.010098313228859013 1104 70-71 0.06960561372811469 1104 72-73 -0.30992838096890907 1104 74-75 -0.4665564369795909 1104 76-77 -0.4043601477657681 1104 78-79 -0.11561650703539428 1104 80-81 0.12901058495090467 1104 82-83 -0.08766620287606841 1104 84-85 -0.10248979744688924 1104 86-87 -0.07676941238197443 1104 88-89 -0.19669827421155262 1104 90-91 -0.10745917186124387 1104 92-93 -0.23031041457517532 1104 94-95 -0.1651157927269402 1104 96-97 -0.2427592754645005 1104 98-99 -0.11160436829053566 1104 100-101 0.21205718077738922 1105 1 0.25252748794426694 1105 2 0.20204490231234473 1105 3 0.2763862465301621 1105 4 0.30457217255002433 1105 5 0.42899347040986413 1105 6 0.2879831384527449 1105 7 0.3305184616320602 1105 8 -0.11306161845741514 1105 9 0.43826765182189575 1105 10-11 0.4361938596610244 1105 12-13 0.3695331172213301 1105 14-15 0.45197135001827604 1105 16-17 0.39553946925006045 1105 18-19 0.1800641103345768 1105 20-21 -0.016113063267617633 1105 22-23 -0.053213292217421326 1105 24-25 -6.566991999790162E-4 1105 26-27 0.11581824452229483 1105 28-29 0.08858126819953327 1105 30-31 0.11897505121192609 1105 32-33 -0.03153932746087662 1105 34-35 0.034427252643055795 1105 36-37 -0.10999429705628927 1105 38-39 -0.015694486185729772 1105 40-41 0.07660227219392368 1105 42-43 0.23251513515663902 1105 44-45 0.30080202419628677 1105 46-47 0.0591887539284599 1105 48-49 0.0833552954622121 1105 50-51 -0.004768317456857574 1105 52-53 0.20805027881970517 1105 54-55 0.10532949082567455 1105 56-57 0.3282535807527154 1105 58-59 0.23610413477418035 1105 60-61 0.18194664262050253 1105 62-63 0.05685038769293982 1105 64-65 0.13801015257421767 1105 66-67 -0.23937612346539083 1105 68-69 0.17483347695289808 1105 70-71 0.172701203679118 1105 72-73 0.05270990760466532 1105 74-75 0.14623185326066945 1105 76-77 0.36406320508629975 1105 78-79 0.34814975703420714 1105 80-81 0.37899391809905225 1105 82-83 0.26960628533676 1105 84-85 -0.01801756257020415 1105 86-87 0.11835775569766938 1105 88-89 0.12133396318367673 1105 90-91 0.1690077888827659 1105 92-93 0.021624792112305613 1105 94-95 0.18650755864362978 1105 96-97 0.16753972785776128 1105 98-99 -0.16181233356422098 1105 100-101 -0.3966912497607282 1106 1 -0.02798928276702739 1106 2 -0.06570824308035483 1106 3 -0.0026929537882907084 1106 4 0.2065834504972841 1106 5 0.17454256758924913 1106 6 0.19351476594466988 1106 7 0.3027999537622108 1106 8 0.2441812271773287 1106 9 0.4289809848156523 1106 10-11 0.33931680211481563 1106 12-13 0.30496837844598446 1106 14-15 0.3369600588649888 1106 16-17 0.18780583104615545 1106 18-19 0.11565076922855866 1106 20-21 0.28635731278344423 1106 22-23 0.16160396466433014 1106 24-25 -0.08298401624462315 1106 26-27 0.07928895827033955 1106 28-29 0.007859426751096521 1106 30-31 0.08718389887285838 1106 32-33 0.0513237769742787 1106 34-35 -0.006253914241511893 1106 36-37 0.046860291377875285 1106 38-39 0.16082859323343257 1106 40-41 -0.03320631587424572 1106 42-43 0.09411596977755465 1106 44-45 -0.017605668503236416 1106 46-47 0.0423784245512806 1106 48-49 0.11351927658890304 1106 50-51 0.36186354189518255 1106 52-53 0.2718094499339756 1106 54-55 0.5086953298517471 1106 56-57 0.24972818461905888 1106 58-59 0.2998892462990739 1106 60-61 0.22243820868553854 1106 62-63 0.22510649406559935 1106 64-65 0.061418496142223944 1106 66-67 0.08986617128936558 1106 68-69 0.0074536848157720215 1106 70-71 0.16825737551594244 1106 72-73 0.2785622151692735 1106 74-75 0.3062073089157735 1106 76-77 0.09429142410260383 1106 78-79 0.07586835923282464 1106 80-81 0.0594948415776706 1106 82-83 0.035619536641803506 1106 84-85 0.1518223237825005 1106 86-87 0.3884639321566077 1106 88-89 0.3716537377001963 1106 90-91 0.5031608391920699 1106 92-93 0.3927099125041096 1106 94-95 0.26619461352976614 1106 96-97 0.337024938050547 1106 98-99 0.3918640357593084 1106 100-101 0.21630140580155555 1107 1 0.45611855364442633 1107 2 0.361664192701717 1107 3 0.48572727462730825 1107 4 0.4554979103387211 1107 5 0.3740970304255953 1107 6 0.37014242217473026 1107 7 0.5497343486629376 1107 8 0.47778198203761946 1107 9 0.6358576285740014 1107 10-11 0.5414895512793763 1107 12-13 0.5181681498589867 1107 14-15 0.7142503828424012 1107 16-17 0.7875620891113115 1107 18-19 0.8817895388549886 1107 20-21 0.8479630417112034 1107 22-23 0.6991717503157631 1107 24-25 0.7997812549498491 1107 26-27 0.7083174770690093 1107 28-29 0.5389971883034619 1107 30-31 0.7213337496463197 1107 32-33 0.5635413189063314 1107 34-35 0.5323122481174991 1107 36-37 0.6591407984729543 1107 38-39 0.7821231317272037 1107 40-41 0.5733017832228242 1107 42-43 0.835368390170153 1107 44-45 0.5900417572544825 1107 46-47 0.49954114033437946 1107 48-49 0.39031792202602844 1107 50-51 0.5723629377272985 1107 52-53 0.42532334353999346 1107 54-55 0.6581403929725198 1107 56-57 0.7740320158868599 1107 58-59 0.5525297463398999 1107 60-61 0.6784286718989563 1107 62-63 0.6488291153829167 1107 64-65 0.45732503888117293 1107 66-67 0.4261650916352764 1107 68-69 0.6632117271945717 1107 70-71 0.5237365399555998 1107 72-73 0.5928224263731892 1107 74-75 0.5349544239521329 1107 76-77 0.6164018485807183 1107 78-79 0.6650558515369553 1107 80-81 0.7239340479459919 1107 82-83 0.5808371836661337 1107 84-85 0.5413798040754401 1107 86-87 0.6498442313674211 1107 88-89 0.8075095564976422 1107 90-91 0.5760434664450713 1107 92-93 0.7602365768524351 1107 94-95 0.7194156920652759 1107 96-97 1.1219481643025127 1107 98-99 1.3555943427956656 1107 100-101 1.4828621232075 1108 1 0.15018135810950284 1108 2 0.2671340448934174 1108 3 0.05465760514278273 1108 4 0.15706025916091448 1108 5 0.316093128772458 1108 6 0.3364664753938129 1108 7 0.36943814703702316 1108 8 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60-61 -1.8889004231584607 2316 62-63 -1.7869787255330074 2316 64-65 -1.6115821757867579 2316 66-67 -2.7539592086219926 2316 68-69 -2.227405628640227 2316 70-71 -1.8023567042241524 2316 72-73 -1.6948513424118765 2316 74-75 -1.6216664317829874 2316 76-77 -2.110563206212028 2316 78-79 -2.22689469417535 2316 80-81 -2.262475199891888 2316 82-83 -2.133163366951095 2316 84-85 -2.2927053010956193 2316 86-87 -2.2989420373780867 2316 88-89 -2.258709760040812 2316 90-91 -2.7011404112292965 2316 92-93 -2.9501507855328164 2316 94-95 -3.0728598443909654 2316 96-97 -3.251258611453874 2316 98-99 -3.862391853800002 2316 100-101 -4.389873779883867 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3521.0 3 1337.0 4 1342.0 5 1507.0 6 1632.0 7 1897.0 8 2516.0 9 2916.0 10 3734.0 11 4716.0 12 5807.0 13 7033.0 14 7814.0 15 9047.0 16 10927.0 17 13293.0 18 12015.0 19 8955.0 20 9163.0 21 9798.0 22 11010.0 23 12243.0 24 14115.0 25 16340.0 26 19045.0 27 22410.0 28 26926.0 29 32609.0 30 39554.0 31 47780.0 32 60894.0 33 77764.0 34 102973.0 35 139913.0 36 196864.0 37 258670.0 38 205618.0 39 32767.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.84883549302521 18.084739012863466 14.223819827948725 22.842605666162598 2 15.561692816550277 19.39816165285551 40.45477614506833 24.585369385525887 3 19.897226035885254 23.753930084185257 29.443466661468324 26.90537721846116 4 12.703835761441823 15.905178417226107 37.16719128666537 34.2237945346667 5 13.839315563083643 36.82107914150081 34.21529898147942 15.124306313936126 6 32.450784124423414 35.072449836794576 17.528249236911872 14.94851680187014 7 29.160614083589014 32.13737077183839 20.430967127336135 18.271048017236456 8 26.716788629180343 33.85523401274426 19.60543055239305 19.822546805682347 9 26.25416278789643 16.950475443816025 20.599803514322495 36.195558253965046 10-11 25.381537768277855 25.965358024768744 27.049003640127133 21.604100566826265 12-13 26.47404894535808 23.360772049490674 27.59152748531984 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22.27491170653361 26.08846542370073 29.213528515326132 22.423094354439527 40-41 22.44153484413643 26.086815774496884 29.197195134473773 22.274454246892912 42-43 22.218118784655385 26.166318009836647 28.72346350241739 22.892099703090572 44-45 22.51891723684948 26.095501110546838 28.592795664603372 22.792785988000315 46-47 22.79958689552404 26.424380264967468 28.27088904831074 22.505143791197757 48-49 22.861905537618014 26.48472013956474 28.045947485008348 22.6074268378089 50-51 22.398946845933054 26.70742463870926 28.31768244843005 22.575946066927635 52-53 22.822207780861362 26.861894218462673 27.75238163527643 22.563516365399536 54-55 22.431559777115027 26.457531000336942 28.04085946049807 23.070049762049962 56-57 22.67060325199913 26.794306338693698 27.815929595364093 22.719160813943088 58-59 23.0400985215502 26.493295563954423 27.731706994459206 22.734898920036173 60-61 22.68983701280054 26.48812621283187 28.47390725480129 22.348129519566296 62-63 22.410826043431708 26.661095107284428 28.393128394625126 22.534950454658738 64-65 22.281505037840365 26.919047164879018 28.05975791354811 22.739689883732506 66-67 22.29540049002869 26.904900391347674 27.634965868899396 23.164733249724232 68-69 22.28521163898687 26.699208749116277 28.095515409568318 22.920064202328536 70-71 22.47901967910239 26.6624476275215 27.91729847822182 22.94123421515429 72-73 22.504128284527216 26.555834178018866 28.09224522984729 22.847792307606625 74-75 22.234935057792544 27.288223381422426 27.6892172335462 22.78762432723883 76-77 22.478082121687518 26.824935091335078 27.658244348969312 23.038738438008092 78-79 22.53155080772505 26.834751937269214 27.72905564328132 22.90464161172442 80-81 22.663683259345806 27.064359922307002 27.51695400007868 22.75500281826851 82-83 22.62542918962872 26.92839460105205 27.813785306329315 22.632390902989915 84-85 22.48639187839594 27.01455373680742 27.52030905761169 22.97874532718495 86-87 22.495251071010976 27.002251846921748 27.654239678802877 22.848257403264395 88-89 22.395486511295033 26.950676532497265 27.33674439425357 23.317092561954126 90-91 22.45878989381607 26.778832279663046 27.607124693271768 23.155253133249115 92-93 22.635985550387574 26.85494654151653 27.762764294265512 22.74630361383038 94-95 22.364201349136263 26.689283385796646 27.817520252882115 23.128995012184973 96-97 22.145867112405384 26.72420334763512 28.013940241587225 23.11598929837227 98-99 22.180018027496633 26.887360176361696 27.40997653779455 23.522645258347115 100-101 22.098641016872897 26.56184163699209 27.85988537823858 23.479631967896434 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 113.0 1 96.0 2 156.0 3 229.0 4 194.0 5 194.5 6 316.5 7 599.5 8 1061.0 9 1390.5 10 1307.5 11 1196.5 12 1263.0 13 1353.5 14 1530.0 15 1658.0 16 1684.0 17 1757.5 18 1880.0 19 2047.5 20 2311.0 21 2682.5 22 3186.5 23 3754.0 24 4460.0 25 5641.0 26 7202.5 27 9053.5 28 10863.5 29 12884.5 30 15615.5 31 18751.5 32 22058.5 33 24431.5 34 26722.0 35 29290.5 36 32054.5 37 35138.0 38 37159.0 39 38360.5 40 39727.5 41 42558.5 42 44938.0 43 46943.5 44 49193.5 45 51140.5 46 52317.0 47 55560.0 48 63667.5 49 63486.5 50 56229.5 51 52274.5 52 49633.5 53 49056.0 54 48338.5 55 45372.0 56 41835.0 57 37740.5 58 33997.5 59 30073.5 60 25551.5 61 21388.5 62 17674.0 63 13826.0 64 10296.5 65 8116.0 66 6234.0 67 4939.5 68 3675.5 69 2372.0 70 1729.0 71 1254.5 72 941.5 73 785.5 74 590.5 75 460.5 76 343.0 77 223.5 78 140.0 79 80.0 80 43.5 81 32.0 82 26.0 83 19.0 84 16.5 85 11.0 86 3.5 87 2.5 88 5.0 89 5.5 90 4.0 91 2.5 92 2.5 93 1.5 94 0.5 95 0.5 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010999154875062131 2 0.005221117820440885 3 0.008214558704160326 4 0.0357820607960882 5 0.04636352624551506 6 0.05986881767438882 7 0.03118747711410022 8 0.026244818910749517 9 0.016498732312593196 10-11 0.0468160231232866 12-13 0.042291054345571163 14-15 0.019840247717675362 16-17 0.023390607835575165 18-19 0.02958633431583168 20-21 0.02384310471334671 22-23 0.015350086392096202 24-25 0.0037940122828537103 26-27 0.002819403623038078 28-29 0.0017403726068136284 30-31 0.006717838262300606 32-33 0.0011138384683607222 34-35 0.0019492173196312638 36-37 0.0049774656554869776 38-39 0.004942658203350705 40-41 0.002889018527310623 42-43 6.265341384529062E-4 44-45 0.0013574906333146302 46-47 0.002332099293130262 48-49 5.569192341803611E-4 50-51 0.0018796024153587185 52-53 9.398012076793593E-4 54-55 0.002645366362356715 56-57 0.0024017141974028073 58-59 0.00257575145808417 60-61 8.00571399134269E-4 62-63 8.353788512705416E-4 64-65 0.003132670692264531 66-67 0.0019144098674949914 68-69 0.005395155081122248 70-71 0.008910707746885778 72-73 0.0029238259794468957 74-75 0.0029934408837194405 76-77 0.002262484388857717 78-79 0.034563799971318664 80-81 0.021476197968080174 82-83 0.003202285596537076 84-85 0.0074836022092986025 86-87 0.006787453166573151 88-89 0.004768620942669342 90-91 0.010128968571655317 92-93 0.012008570987014036 94-95 0.005116695464032067 96-97 0.003968049543535072 98-99 0.02328618547916635 100-101 0.043230855553250534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1436474.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.9933733230688 #Duplication Level Percentage of deduplicated Percentage of total 1 83.32002127781428 53.319292269172045 2 10.224093836667612 13.085485075599145 3 2.7679574536204994 5.313928040157225 4 1.1600075857196879 2.969311939622069 5 0.6382374714800956 2.0421484390598614 6 0.3828773657958938 1.4700968523797882 7 0.25868142754578904 1.1587728015277434 8 0.18886254700935093 0.966876118201202 9 0.14461572804585215 0.8329003445903048 >10 0.8172764863866302 9.707925545982727 >50 0.06308109906861224 2.786502032587132 >100 0.031239987088935602 3.7482009307469153 >500 0.001959257415078133 0.8158025560687179 >1k 9.796287075390666E-4 1.4337144818615455 >5k 1.088476341710074E-4 0.34904257244367703 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5011 0.34884028530972366 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4095 0.28507303299607234 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3975 0.2767192444833669 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3064 0.21330006669107832 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2356 0.16401271446611634 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1830 0.1273952748187576 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1827 0.12718643010593997 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 6.961490427254514E-5 16-17 0.0 0.0 0.0 0.0 6.961490427254514E-5 18-19 0.0 0.0 0.0 3.480745213627257E-5 6.961490427254514E-5 20-21 0.0 0.0 0.0 6.961490427254514E-5 1.3922980854509027E-4 22-23 0.0 0.0 0.0 1.044223564088177E-4 1.7403726068136282E-4 24-25 0.0 0.0 0.0 5.221117820440886E-4 2.088447128176354E-4 26-27 0.0 0.0 0.0 9.049937555430867E-4 2.088447128176354E-4 28-29 0.0 6.961490427254514E-5 0.0 0.0017055651546773556 2.088447128176354E-4 30-31 0.0 6.961490427254514E-5 0.0 0.002819403623038078 2.088447128176354E-4 32-33 0.0 6.961490427254514E-5 0.0 0.004838235846941887 2.088447128176354E-4 34-35 0.0 6.961490427254514E-5 0.0 0.00664822335802806 2.088447128176354E-4 36-37 0.0 6.961490427254514E-5 0.0 0.009537241885338683 2.088447128176354E-4 38-39 0.0 6.961490427254514E-5 0.0 0.013783751045963938 2.088447128176354E-4 40-41 0.0 6.961490427254514E-5 0.0 0.01924852103135873 2.088447128176354E-4 42-43 0.0 6.961490427254514E-5 0.0 0.02739346483124651 2.4365216495390797E-4 44-45 0.0 6.961490427254514E-5 0.0 0.03393726583286576 2.7845961709018055E-4 46-47 0.0 6.961490427254514E-5 0.0 0.047790631783102236 2.7845961709018055E-4 48-49 0.0 6.961490427254514E-5 0.0 0.0625489914888818 2.7845961709018055E-4 50-51 0.0 6.961490427254514E-5 0.0 0.06930163720331868 2.7845961709018055E-4 52-53 0.0 6.961490427254514E-5 0.0 0.07960464303565537 3.828819734989983E-4 54-55 0.0 6.961490427254514E-5 0.0 0.10041949941314635 4.8730432990781595E-4 56-57 0.0 6.961490427254514E-5 0.0 0.13098044238879367 4.8730432990781595E-4 58-59 0.0 6.961490427254514E-5 0.0 0.1665884659242005 4.8730432990781595E-4 60-61 0.0 6.961490427254514E-5 0.0 0.21002816619026865 4.8730432990781595E-4 62-63 0.0 6.961490427254514E-5 0.0 0.25221479817943104 4.8730432990781595E-4 64-65 0.0 6.961490427254514E-5 0.0 0.29850870952067354 4.8730432990781595E-4 66-67 0.0 6.961490427254514E-5 0.0 0.36763630946331083 4.8730432990781595E-4 68-69 0.0 6.961490427254514E-5 0.0 0.44831998351519065 4.8730432990781595E-4 70-71 0.0 1.044223564088177E-4 0.0 0.4896364292009462 4.8730432990781595E-4 72-73 0.0 1.3922980854509027E-4 0.0 0.5515240790992388 4.8730432990781595E-4 74-75 0.0 1.3922980854509027E-4 0.0 0.6166836294983411 4.8730432990781595E-4 76-77 0.0 1.3922980854509027E-4 0.0 0.6847670058768902 4.8730432990781595E-4 78-79 0.0 1.3922980854509027E-4 0.0 0.7689662325945336 5.221117820440886E-4 80-81 0.0 1.3922980854509027E-4 0.0 0.8930547994603453 5.569192341803611E-4 82-83 0.0 1.3922980854509027E-4 0.0 1.0272723348978123 5.569192341803611E-4 84-85 0.0 1.3922980854509027E-4 0.0 1.2305130479215078 5.569192341803611E-4 86-87 0.0 1.3922980854509027E-4 0.0 1.5267940805054598 5.917266863166336E-4 88-89 0.0 1.3922980854509027E-4 0.0 1.8508862673462936 6.265341384529062E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1275 0.0 28.329956 2 GTACCTG 1370 0.0 26.714245 1 GTACATG 6080 0.0 24.937899 1 TACATGG 6270 0.0 23.649912 2 ACATGGG 5955 0.0 23.224892 3 GTATCAA 10535 0.0 22.19746 1 GAGTACT 4790 0.0 21.23087 12-13 CATGGGG 2965 0.0 20.357363 4 AGAGTAC 8115 0.0 20.17497 10-11 AGTACTT 5095 0.0 20.006569 12-13 TATAACG 120 0.0047483 19.80301 2 GTACTTT 5240 0.0 19.494902 14-15 ATCAACG 11960 0.0 19.233425 3 TATCAAC 11965 0.0 19.22539 2 TCAACGC 11945 0.0 19.21779 4 CAACGCA 12040 0.0 19.06682 5 ATAACGC 125 0.00601193 19.01089 3 AACGCAG 12245 0.0 18.74761 6 AGTACAT 4650 0.0 18.704264 2 GAGTACA 5015 0.0 18.671017 1 >>END_MODULE