FastQCFastQC Report
Thu 26 May 2016
SRR936622_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936622_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436474
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT53980.37578125326319867No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53360.3714651291983008No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47600.33136694433731484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28180.19617480024003217No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18790.1308064051281123No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17760.12363606998804016No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14410.10031507705673755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGC7000.025.3845841
TACCTGG13300.025.354412
GTACCTG13850.025.3127571
GTATAGA4100.024.5981141
GTAAGGT5500.024.1783714
TACATCG1401.7832885E-523.7441355
GTACATG59850.023.5911731
TACATGG62650.022.2881012
TATCACG1503.0364186E-522.1642782
GAGTACT58100.021.98605712-13
ACATGGG59700.021.7975643
GTACTTT59750.021.02127314-15
TAAGGTG6350.020.9397095
GGGCGTA4500.020.0506049
AGGTAAG6600.019.4297242
AGTACTT60050.019.37422412-13
GTACACG2001.1688733E-519.2099551
GTAGGAC3759.094947E-1218.9972923
ATCACGC1507.7297474E-418.9972923
CATGGGG30700.018.8735314
Produced by FastQC (version 0.11.5)