##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936622_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1436474 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.52855394528547 31.0 31.0 34.0 30.0 34.0 2 31.765032990503133 31.0 31.0 34.0 30.0 34.0 3 32.04102267078972 33.0 31.0 34.0 30.0 34.0 4 35.8302294368015 37.0 35.0 37.0 35.0 37.0 5 35.64593859686983 37.0 35.0 37.0 33.0 37.0 6 35.75347065105251 37.0 35.0 37.0 33.0 37.0 7 35.69807111023242 37.0 35.0 37.0 33.0 37.0 8 35.67454823407872 37.0 35.0 37.0 33.0 37.0 9 37.325105083698 39.0 37.0 39.0 34.0 39.0 10-11 37.205966136525966 39.0 37.0 39.0 33.0 39.0 12-13 37.08074284672051 39.0 37.0 39.0 33.0 39.0 14-15 38.227427019215106 40.0 38.0 41.0 33.0 41.0 16-17 38.153066466918304 40.0 37.0 41.0 33.0 41.0 18-19 38.20231518287139 40.0 37.5 41.0 33.5 41.0 20-21 38.26963314337746 40.0 38.0 41.0 34.0 41.0 22-23 38.18558880982182 40.0 37.5 41.0 33.0 41.0 24-25 38.07842153773754 40.0 37.0 41.0 33.0 41.0 26-27 37.881496636903975 40.0 37.0 41.0 33.0 41.0 28-29 37.80684300586019 40.0 37.0 41.0 33.0 41.0 30-31 37.811583432766625 40.0 37.0 41.0 33.0 41.0 32-33 37.655036916783736 40.0 37.0 41.0 33.0 41.0 34-35 37.494481278463795 40.0 36.0 41.0 32.0 41.0 36-37 37.27330985454662 39.5 36.0 41.0 31.5 41.0 38-39 37.122876223307905 39.0 36.0 41.0 31.0 41.0 40-41 36.91172238411555 39.0 36.0 41.0 31.0 41.0 42-43 36.69052137386406 39.0 35.0 41.0 30.5 41.0 44-45 36.42380126615588 39.0 35.0 40.0 30.0 41.0 46-47 36.04031816795849 39.0 35.0 40.0 29.5 41.0 48-49 35.59173817277584 38.5 35.0 40.0 28.0 41.0 50-51 35.40004413584931 38.0 35.0 40.0 27.5 41.0 52-53 35.13046424787362 38.0 34.0 40.0 27.0 41.0 54-55 34.86134312211707 38.0 34.0 40.0 26.0 41.0 56-57 34.5829764409241 38.0 34.0 40.0 26.0 41.0 58-59 34.212791877889885 37.5 33.0 40.0 25.5 41.0 60-61 33.82618411471422 37.0 33.0 40.0 24.0 41.0 62-63 33.53831813175874 36.5 32.5 39.0 23.5 40.5 64-65 33.10953278653146 36.0 32.0 39.0 22.5 40.0 66-67 32.70679420581229 35.5 31.5 39.0 22.0 40.0 68-69 32.23836421682537 35.0 31.0 38.0 21.0 40.0 70-71 31.765781698798584 35.0 31.0 37.5 20.0 39.5 72-73 31.338883961700663 34.5 30.0 37.0 19.0 39.0 74-75 30.967414307533588 34.0 30.0 36.5 18.0 39.0 76-77 29.54950907569507 32.5 28.5 35.0 16.0 37.0 78-79 30.3624694912682 34.0 30.0 35.0 17.5 37.5 80-81 30.200060356122005 34.0 30.0 35.0 14.0 37.0 82-83 29.859595091870787 34.0 29.5 35.0 9.5 37.0 84-85 29.4235913772195 34.0 29.0 35.0 7.0 36.0 86-87 29.042443859060448 34.0 29.0 35.0 3.5 36.0 88-89 28.634372428599477 33.5 29.0 35.0 2.0 35.0 90-91 28.186694294501677 33.0 27.5 35.0 2.0 35.0 92-93 27.72890946860159 33.0 27.0 35.0 2.0 35.0 94-95 27.188970005722346 33.0 26.0 34.5 2.0 35.0 96-97 26.501926592475744 32.0 25.0 34.0 2.0 35.0 98-99 25.63786535642135 32.0 23.5 34.0 2.0 35.0 100-101 22.788849293478336 29.0 9.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.43177332650989797 1101 2 0.40487827645356944 1101 3 0.23498307815612662 1101 4 0.14756410492826433 1101 5 0.184922985522185 1101 6 0.15631243794154415 1101 7 0.22416215761333547 1101 8 0.035964007587644176 1101 9 0.20876886144363027 1101 10-11 0.21526924163269712 1101 12-13 0.1827081171523659 1101 14-15 0.32122097402930194 1101 16-17 0.31693729656826974 1101 18-19 0.22191357625415975 1101 20-21 0.08338262804145558 1101 22-23 -7.618392823829367E-4 1101 24-25 0.012271855317386837 1101 26-27 0.1469449920700967 1101 28-29 0.10597527913267868 1101 30-31 0.033539205106698944 1101 32-33 0.21323763802463702 1101 34-35 0.2818504588733717 1101 36-37 0.3449555894788219 1101 38-39 0.2613671660675223 1101 40-41 0.2894302798628061 1101 42-43 0.31464790564908895 1101 44-45 0.3680455436742065 1101 46-47 0.5061038681775969 1101 48-49 0.497497061526893 1101 50-51 0.45977549413107965 1101 52-53 0.3961286267780224 1101 54-55 0.37457550879930324 1101 56-57 0.36195506533141497 1101 58-59 0.5318590074429466 1101 60-61 0.47441190544317635 1101 62-63 0.40418084621122574 1101 64-65 0.41945572854412205 1101 66-67 0.32359737997898463 1101 68-69 0.13722461384465845 1101 70-71 0.24798252316599445 1101 72-73 0.29830627410369104 1101 74-75 0.29855842048981174 1101 76-77 0.5486981715415844 1101 78-79 0.5936977322502841 1101 80-81 0.35599444850805284 1101 82-83 0.3819679931984332 1101 84-85 0.6453268643763366 1101 86-87 0.45115512346784925 1101 88-89 0.3875310101763745 1101 90-91 0.32754810618810737 1101 92-93 0.5304192350655121 1101 94-95 0.21394117772950594 1101 96-97 0.167429118504959 1101 98-99 0.14320458261841296 1101 100-101 0.2622979148235416 1102 1 0.4131800931313414 1102 2 0.4166093018388146 1102 3 0.1362772439369806 1102 4 0.006013796285508022 1102 5 0.06540176841291867 1102 6 0.019334510939124527 1102 7 0.020395018523167607 1102 8 -0.05487341502094978 1102 9 -0.02117663073340026 1102 10-11 0.09779385069406743 1102 12-13 0.08269852493889118 1102 14-15 0.1875329245569688 1102 16-17 0.19713991879417847 1102 18-19 0.033736860275681124 1102 20-21 0.12411919258040882 1102 22-23 -0.060458686184780674 1102 24-25 -0.0025295173479378263 1102 26-27 -0.33618096208247294 1102 28-29 -0.32520660181928207 1102 30-31 -0.05469979034524641 1102 32-33 -0.06371529329148018 1102 34-35 0.16938609059054244 1102 36-37 0.20864044807198212 1102 38-39 -0.023979930833689878 1102 40-41 0.11816522351345071 1102 42-43 0.013501342500020996 1102 44-45 -0.058569828421177306 1102 46-47 -0.20256914277346283 1102 48-49 -0.011551360134504307 1102 50-51 -0.011463106725727812 1102 52-53 -0.22125447755869487 1102 54-55 -0.08607292483139162 1102 56-57 0.08086979167227781 1102 58-59 -0.034708297315660275 1102 60-61 0.15000604239146043 1102 62-63 0.21058369065208637 1102 64-65 0.042720006330142724 1102 66-67 0.03769208896436993 1102 68-69 -0.10792775273338151 1102 70-71 -0.08070725598097894 1102 72-73 -0.07509105020009699 1102 74-75 -0.5880098798454689 1102 76-77 -0.2130712366803671 1102 78-79 0.060710445708494376 1102 80-81 -0.05743792233195322 1102 82-83 -0.17762160548628358 1102 84-85 -0.10784031392165616 1102 86-87 -0.251649298570527 1102 88-89 -0.15472996123239824 1102 90-91 -0.021297626129847202 1102 92-93 0.051811409217602744 1102 94-95 -0.10320592459703803 1102 96-97 -0.1870832908269513 1102 98-99 -0.6927266185588259 1102 100-101 -0.6760879142051799 1103 1 0.4727903315602333 1103 2 0.3505406628793786 1103 3 0.2165557427135738 1103 4 0.11744777317072419 1103 5 -0.027925939262743782 1103 6 0.031364093320213726 1103 7 0.06986672273841776 1103 8 0.12424337012823372 1103 9 -0.012951260529987962 1103 10-11 0.08718284165730239 1103 12-13 0.15783307973692473 1103 14-15 0.10452512972765504 1103 16-17 0.006028591949885254 1103 18-19 0.08306021601303115 1103 20-21 0.07460584164823558 1103 22-23 0.08122646061414684 1103 24-25 -0.01962204802361356 1103 26-27 -0.03485289136232694 1103 28-29 0.032463445316189166 1103 30-31 0.055824852017160254 1103 32-33 0.0805605665193525 1103 34-35 -0.006025429236025559 1103 36-37 0.10975850445129964 1103 38-39 0.061143086387659196 1103 40-41 0.26801312683102907 1103 42-43 0.2639803515494208 1103 44-45 0.3829577501783845 1103 46-47 0.44378761679026724 1103 48-49 0.44750690132473636 1103 50-51 0.12563831624206045 1103 52-53 0.2817215393895083 1103 54-55 0.48723606767133987 1103 56-57 0.30032788459156023 1103 58-59 0.34931737199734414 1103 60-61 0.34843800041616646 1103 62-63 0.2734624783132915 1103 64-65 0.3271873318957432 1103 66-67 0.35361417456302746 1103 68-69 0.3932719599329957 1103 70-71 0.2116404900843385 1103 72-73 0.16524928102629488 1103 74-75 0.2049130217416142 1103 76-77 0.2525722323820112 1103 78-79 0.46934831946264666 1103 80-81 0.5037209995293246 1103 82-83 0.5128035557942177 1103 84-85 0.32384600185753953 1103 86-87 0.25560900614385673 1103 88-89 0.33852240896843 1103 90-91 0.14634118022354414 1103 92-93 0.4481813090385778 1103 94-95 0.19679002561876402 1103 96-97 0.016660346918992985 1103 98-99 -0.05477143789393324 1103 100-101 0.17180894095069732 1104 1 0.21458543849983513 1104 2 0.27205357202367253 1104 3 -0.010196130756852995 1104 4 -0.08684988870682986 1104 5 -0.19750237124893744 1104 6 -0.17961951709200008 1104 7 -0.07356010775780675 1104 8 -0.15808285037844172 1104 9 -0.08856506364632111 1104 10-11 -0.16360293365406875 1104 12-13 -0.221326042702934 1104 14-15 -0.02033883301577788 1104 16-17 -0.12374130385688176 1104 18-19 -0.08552996470474028 1104 20-21 -0.24595370634330038 1104 22-23 -0.04730455407154466 1104 24-25 -0.01704299266632603 1104 26-27 -0.10902100658083924 1104 28-29 -0.17679456452732722 1104 30-31 -0.11078468653843743 1104 32-33 -0.18833527220270696 1104 34-35 -0.18065973395827228 1104 36-37 -0.3010013567047878 1104 38-39 -0.27829285566544115 1104 40-41 -0.28847421484572777 1104 42-43 -0.2538510050608238 1104 44-45 -0.2861057346785927 1104 46-47 -0.40290826811664004 1104 48-49 -0.42357695912470206 1104 50-51 -0.3244336742219147 1104 52-53 -0.4658732013272484 1104 54-55 -0.4022362615216366 1104 56-57 -0.36304057574705695 1104 58-59 -0.37574462481554605 1104 60-61 -0.4772291624661733 1104 62-63 -0.6050534629921742 1104 64-65 -0.5604879489062569 1104 66-67 -0.42461766367187437 1104 68-69 -0.4213839479577999 1104 70-71 -0.43608240451112934 1104 72-73 -0.33731078187190633 1104 74-75 -0.5257814450616749 1104 76-77 -0.29248210112436723 1104 78-79 -0.3948955425061911 1104 80-81 -0.3159077672330248 1104 82-83 -0.34563991872845534 1104 84-85 -0.23275454090473602 1104 86-87 -0.15165202199332128 1104 88-89 -0.13445644669671708 1104 90-91 -0.1250206635040918 1104 92-93 -0.104821368596685 1104 94-95 -0.1571826843277968 1104 96-97 -0.4555475053474112 1104 98-99 -0.42149502164434693 1104 100-101 -0.285812250533688 1105 1 0.355983305883548 1105 2 0.35733970483255817 1105 3 0.16357987416171937 1105 4 -0.016962075138529542 1105 5 -0.0718750494638769 1105 6 0.024964237542313583 1105 7 0.1522664755005252 1105 8 0.09225696821214058 1105 9 -0.04506643218004314 1105 10-11 -0.005061357266178845 1105 12-13 -0.0069921797340839476 1105 14-15 0.09533097072278451 1105 16-17 0.057929233223163124 1105 18-19 0.09529334073939566 1105 20-21 0.06387288432252092 1105 22-23 -0.005158715414076198 1105 24-25 -0.048324821723689126 1105 26-27 -0.04126562411362755 1105 28-29 -0.14996463678739502 1105 30-31 -0.105401293389626 1105 32-33 -0.04509293988049734 1105 34-35 -0.08438181235173658 1105 36-37 9.158868201453174E-4 1105 38-39 -0.02430207058264955 1105 40-41 -0.12558444199639496 1105 42-43 -0.08615099357921707 1105 44-45 -0.13925502893001607 1105 46-47 0.12891986330705407 1105 48-49 0.06283307506085833 1105 50-51 -0.012048721796517725 1105 52-53 -0.05253249754165523 1105 54-55 -0.06572048542042097 1105 56-57 0.004782029689295086 1105 58-59 0.07706596196875637 1105 60-61 0.14157586129334732 1105 62-63 0.045012093291788347 1105 64-65 -0.10350879728605378 1105 66-67 -0.08962412670664577 1105 68-69 -0.26555906842209964 1105 70-71 -0.2640027147367974 1105 72-73 -0.18679429465167985 1105 74-75 -0.1013915444331488 1105 76-77 -0.23661110996520662 1105 78-79 -0.11010904487844186 1105 80-81 -0.3102002964092776 1105 82-83 -0.13522369188148886 1105 84-85 -0.049644173011763826 1105 86-87 -0.2024442530181112 1105 88-89 -0.08984298802865709 1105 90-91 -0.29835221903989506 1105 92-93 -0.033926136427439246 1105 94-95 -0.06839836450201986 1105 96-97 -0.21896895077087564 1105 98-99 -0.026937859898108485 1105 100-101 0.2531205894608348 1106 1 0.3223352917870592 1106 2 0.333340580203501 1106 3 0.10930498881833728 1106 4 0.01715475292465385 1106 5 -0.1242077055166746 1106 6 -0.15012787449762044 1106 7 -0.06806193953094919 1106 8 0.10267086397130498 1106 9 0.0026978817385412412 1106 10-11 -0.13351874235959116 1106 12-13 -0.10192747963353099 1106 14-15 -0.10337351800587413 1106 16-17 0.05810609965928393 1106 18-19 0.0033775046375126294 1106 20-21 -4.277339082108256E-4 1106 22-23 5.703421849574397E-4 1106 24-25 -0.09037564228962225 1106 26-27 -0.2381420213584704 1106 28-29 -0.019640013772310283 1106 30-31 -0.06605818004984343 1106 32-33 0.049363169887996605 1106 34-35 -0.16023911934093604 1106 36-37 -0.11167539984308661 1106 38-39 -0.12813398804119913 1106 40-41 -0.035417461214940715 1106 42-43 -0.19926957804402434 1106 44-45 -0.1906821288298417 1106 46-47 -0.08111634007331503 1106 48-49 -0.04559878466849199 1106 50-51 -0.20757385946934903 1106 52-53 -0.23105298857947076 1106 54-55 -0.27740980042378993 1106 56-57 -0.24920807710973492 1106 58-59 -0.3907268958344048 1106 60-61 -0.06853816331891949 1106 62-63 -0.5235234148855739 1106 64-65 -0.3129507294410274 1106 66-67 -0.31721868657805175 1106 68-69 -0.21966859543879735 1106 70-71 -0.2534496840478795 1106 72-73 -0.12608996062219902 1106 74-75 -0.1346584703152658 1106 76-77 -0.20984909578193012 1106 78-79 -0.039922311653395326 1106 80-81 -0.16661540712295064 1106 82-83 0.059722267168506704 1106 84-85 0.07713038689738028 1106 86-87 -0.03133139996912604 1106 88-89 -0.049176913746642015 1106 90-91 -0.16570710838281855 1106 92-93 -0.08670732576225149 1106 94-95 0.07013286114936435 1106 96-97 -0.18352444532716916 1106 98-99 -0.10317767003316547 1106 100-101 0.16734791424043394 1107 1 0.5434156314561456 1107 2 0.5713443860777581 1107 3 0.37515509294088645 1107 4 0.1876203027264438 1107 5 0.05518662915218897 1107 6 0.20303407271357798 1107 7 0.11793426541975549 1107 8 0.12502304580892343 1107 9 0.12100518330694143 1107 10-11 0.2766302857171823 1107 12-13 0.2663280651120985 1107 14-15 0.45673814141935765 1107 16-17 0.38275263100497625 1107 18-19 0.30891099682708756 1107 20-21 0.34929052404959293 1107 22-23 0.2503370418226325 1107 24-25 0.2836464469359825 1107 26-27 0.32720959352205625 1107 28-29 0.35409340994941374 1107 30-31 0.36661216980824207 1107 32-33 0.21355949129718965 1107 34-35 0.15888285040421835 1107 36-37 0.30662639903913913 1107 38-39 0.24282599164396146 1107 40-41 0.2706184893673296 1107 42-43 0.20356042638969996 1107 44-45 0.34964218686378246 1107 46-47 0.4109877563156985 1107 48-49 0.5737265138409526 1107 50-51 0.30156150164893347 1107 52-53 0.21162074858787605 1107 54-55 0.36183522201319107 1107 56-57 0.3664484632612641 1107 58-59 0.29448855913625493 1107 60-61 0.12949561866375348 1107 62-63 0.16650192904359074 1107 64-65 0.29456724787978317 1107 66-67 0.3296446244423379 1107 68-69 0.5038836681272372 1107 70-71 0.1698140042222427 1107 72-73 0.04390876293502899 1107 74-75 0.3380148293434324 1107 76-77 0.30725907124173446 1107 78-79 0.42642019267320563 1107 80-81 0.2402468935681803 1107 82-83 0.11922885663519267 1107 84-85 0.3081133064693091 1107 86-87 0.485127269670798 1107 88-89 0.665850020097988 1107 90-91 0.4668963522655325 1107 92-93 0.500044776872766 1107 94-95 0.6698428876299154 1107 96-97 0.6405231050979623 1107 98-99 0.679472991935473 1107 100-101 0.5631552282124304 1108 1 0.4623865760657253 1108 2 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#Quality Count 2 1.0 3 7.0 4 17.0 5 43.0 6 101.0 7 224.0 8 592.0 9 1048.0 10 1715.0 11 2717.0 12 3808.0 13 5209.0 14 6717.0 15 8872.0 16 10939.0 17 14252.0 18 12756.0 19 8906.0 20 8344.0 21 8543.0 22 8966.0 23 10162.0 24 12101.0 25 14366.0 26 17851.0 27 22305.0 28 27726.0 29 34017.0 30 42348.0 31 52863.0 32 66550.0 33 84434.0 34 111723.0 35 152203.0 36 216202.0 37 282042.0 38 175325.0 39 10479.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.49025225523288 19.179492955366978 14.67486985995206 22.65538492944808 2 15.85449061690197 20.635157132254548 39.204180724720224 24.30617152612326 3 20.311874526162775 23.99327212058387 29.537158932923795 26.15769442032956 4 13.340694702529438 16.751129791475062 36.36713645291079 33.54103905308471 5 13.815565057216489 37.462425355418894 33.74276179032826 14.979247797036354 6 32.563833386472716 35.88321125199621 16.734657223172853 14.81829813835823 7 29.191896268223445 32.67869797852241 19.810104464125352 18.3193012891288 8 26.967143157481445 34.4293039762641 18.94005739052708 19.663495475727373 9 26.289924750473574 17.0280161596078 20.04551590376359 36.63654318615504 10-11 25.545338762848775 26.473969687487276 26.4831240760768 21.497567473587154 12-13 26.623535517411934 23.77140792560103 27.19883408755412 22.40622246943291 14-15 22.91363043845253 25.360875338720373 25.967848322888187 25.757645899938915 16-17 22.318677539586513 28.720290099229086 26.583147345514085 22.377885015670316 18-19 22.163966129552687 27.064717866358134 29.06753935941039 21.70377664467879 20-21 22.94177372572484 25.872527408267537 29.308546241890298 21.877152624117326 22-23 22.485376402098904 26.022572813421863 29.24298406676123 22.249066717718005 24-25 22.817990440481346 26.13308698939208 29.215391298415426 21.83353127171115 26-27 22.470612068161344 26.181852229835002 29.37783767753541 21.969698024468247 28-29 22.014286370654812 26.8371721312046 29.238329409373232 21.910212088767356 30-31 22.230498669483755 26.22430989803146 29.2680855359222 22.277105896562585 32-33 22.10772396777526 26.465248132151498 29.22772157291919 22.199306327154048 34-35 22.828061535805443 26.306915346672334 28.963263623987924 21.901759493534296 36-37 22.818146471799775 25.96361056742272 29.04756816100432 22.170674799773188 38-39 22.36791863401492 26.448574546327897 29.15645792035699 22.027048899300194 40-41 22.523793511790764 26.486942198700795 28.974792209247845 22.014472080260592 42-43 22.410811322633222 26.576453952457307 28.535398719913335 22.47733600499614 44-45 22.610070423907928 26.427866314315075 28.430589706246817 22.531473555530184 46-47 23.048128640070395 26.879679095988564 27.941016762250058 22.131175501690983 48-49 23.14581419449435 26.889954185144095 27.687963270138464 22.276268350223084 50-51 22.627401853921615 27.13035475542972 27.933226520456245 22.30901687019242 52-53 23.14073981252328 27.325863503301555 27.309782135758766 22.223614548416403 54-55 22.64936497172624 27.0587420299461 27.558145363757507 22.733747634570157 56-57 22.94443479462536 27.329946884997785 27.32834567253186 22.397272647844993 58-59 23.456962014741876 26.885478943953572 27.179780459075552 22.477778582228993 60-61 22.88238783276315 27.021467826803736 28.03108783039609 22.065056510037024 62-63 22.671755725190838 27.139823797440155 27.956057267572394 22.232363209796613 64-65 22.43442744798914 27.454325241807542 27.57241345216888 22.538833858034437 66-67 22.63810149366365 27.31001657228366 27.312348735706358 22.739533198346322 68-69 22.57778869960177 27.145195710461362 27.554984557646982 22.722031032289884 70-71 22.821277791062794 27.20843075194463 27.447421796219935 22.522869660772646 72-73 22.826874286229437 26.983268400008768 27.631418191842734 22.55843912191906 74-75 22.575730573612887 27.852923199445307 27.080058532211517 22.491287694730293 76-77 22.758414722906487 27.297051666410827 27.14800423681764 22.796529373865045 78-79 22.804061639617732 27.445935488452577 27.106316748432533 22.643686123497154 80-81 23.02355731137232 27.594757707962863 26.937486858064307 22.444198122600508 82-83 22.877714571368994 27.602947713899507 27.101032897386652 22.41830481734485 84-85 22.81137801467471 27.60718321243653 26.95163222865752 22.629806544231243 86-87 22.78124130805523 27.518988690158952 26.955393853230714 22.744376148555105 88-89 22.528266832492253 27.684574390671056 26.652296199219105 23.134862577617586 90-91 22.729945655523075 27.522732313151476 26.84385284898544 22.90346918234001 92-93 22.869949381558346 27.405315806584785 27.065411334883006 22.659323476973864 94-95 22.585457991059275 27.38460499502558 27.12516247792145 22.90477453599369 96-97 22.4794344155322 27.26468588639227 27.345447861704862 22.910431836370666 98-99 22.464783192011232 27.540716986570512 26.599559846468434 23.394939974949818 100-101 21.967147030042643 27.649387300847973 26.877965283920553 23.50550038518884 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 215.0 1 166.5 2 182.0 3 258.0 4 217.0 5 200.0 6 341.0 7 700.0 8 1128.0 9 1404.0 10 1375.0 11 1281.0 12 1329.5 13 1413.0 14 1631.0 15 1799.5 16 1884.5 17 2070.5 18 2208.5 19 2299.5 20 2531.5 21 2931.0 22 3384.0 23 3959.0 24 4731.0 25 5781.5 26 7270.0 27 9142.0 28 11030.5 29 13288.5 30 16106.5 31 18895.5 32 21972.0 33 24404.5 34 26844.0 35 29242.0 36 32145.5 37 35218.0 38 37088.5 39 38281.0 40 39369.0 41 42083.5 42 44396.5 43 46388.5 44 48915.5 45 51029.0 46 51929.5 47 55477.0 48 64412.0 49 63918.0 50 55977.0 51 51925.5 52 49277.5 53 48443.5 54 47611.0 55 44830.0 56 40955.0 57 37061.0 58 34023.5 59 30682.5 60 26161.0 61 21513.0 62 17630.5 63 13825.0 64 10134.0 65 7874.5 66 6154.5 67 5032.0 68 3779.0 69 2418.5 70 1728.0 71 1271.0 72 1019.5 73 833.0 74 643.5 75 504.5 76 359.0 77 227.0 78 142.5 79 95.5 80 64.5 81 38.5 82 30.5 83 22.5 84 10.5 85 3.5 86 3.5 87 3.0 88 0.5 89 0.5 90 1.5 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.1061112139864695 2 0.011695303917787583 3 0.004385738969170344 4 0.010233390928064134 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0036895899264448922 10-11 3.132670692264531E-4 12-13 1.7403726068136282E-4 14-15 1.044223564088177E-4 16-17 0.0 18-19 3.480745213627257E-5 20-21 3.480745213627257E-5 22-23 8.00571399134269E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 1.044223564088177E-4 32-33 0.004316124064897798 34-35 6.613415905891788E-4 36-37 0.002819403623038078 38-39 1.044223564088177E-4 40-41 0.0036895899264448922 42-43 0.011486459204969946 44-45 0.0020884471281763543 46-47 0.0022276769367214444 48-49 0.010407428188745498 50-51 3.828819734989983E-4 52-53 0.002018832223903809 54-55 0.01113838468360722 56-57 0.0043509315170340715 58-59 0.001531527893995993 60-61 0.006404571193074152 62-63 0.004107279352080163 64-65 0.01806506765872546 66-67 0.0027497887187655327 68-69 1.7403726068136282E-4 70-71 0.001287875729042085 72-73 3.480745213627257E-5 74-75 0.0 76-77 0.001287875729042085 78-79 0.010964347422925859 80-81 0.016150657791230472 82-83 0.012147800795559126 84-85 0.008701863034068143 86-87 0.011033962327198403 88-89 0.029725564124376772 90-91 0.024887328277434884 92-93 0.0028542110751743504 94-95 0.008701863034068143 96-97 0.010755502710108224 98-99 0.010929539970789586 100-101 0.01152126665710622 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1436474.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.83486824902741 #Duplication Level Percentage of deduplicated Percentage of total 1 82.05901029889895 50.74108090478 2 10.880846170993067 13.456313788425813 3 2.99934743288061 5.563927600356933 4 1.319117360371271 3.2626979273424936 5 0.687950963246105 2.1269678587057204 6 0.4050508642625473 1.5027760095497578 7 0.2978707566543702 1.2893159295072758 8 0.21132861276742104 1.045398154217857 9 0.15714307899863186 0.8745229427514217 >10 0.873315431998836 10.007969367942348 >50 0.0708933552841622 3.0310856115091855 >100 0.034741792422116044 4.12691888344094 >500 0.0018047366517060146 0.7629655306679543 >1k 0.001353552488779511 1.4593921531855083 >5k 2.2559208146325183E-4 0.7486673376167676 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5398 0.37578125326319867 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5336 0.3714651291983008 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4760 0.33136694433731484 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2818 0.19617480024003217 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1879 0.1308064051281123 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1776 0.12363606998804016 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1441 0.10031507705673755 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.480745213627257E-5 0.0 20-21 0.0 0.0 0.0 6.961490427254514E-5 0.0 22-23 0.0 0.0 0.0 1.044223564088177E-4 0.0 24-25 0.0 0.0 0.0 5.221117820440886E-4 3.480745213627257E-5 26-27 0.0 0.0 0.0 9.398012076793593E-4 6.961490427254514E-5 28-29 0.0 0.0 0.0 0.0017751800589499009 6.961490427254514E-5 30-31 0.0 0.0 0.0 0.002958633431583168 1.044223564088177E-4 32-33 0.0 0.0 0.0 0.0049774656554869776 1.7403726068136282E-4 34-35 0.0 0.0 0.0 0.00678745316657315 2.088447128176354E-4 36-37 0.0 0.0 0.0 0.009920123858837681 2.088447128176354E-4 38-39 0.0 0.0 0.0 0.014445092636553115 2.088447128176354E-4 40-41 0.0 0.0 0.0 0.020049092430493 2.088447128176354E-4 42-43 0.0 0.0 0.0 0.028228843682517053 2.4365216495390797E-4 44-45 0.0 0.0 0.0 0.034737837232000024 2.7845961709018055E-4 46-47 0.0 0.0 0.0 0.0485912031822365 2.7845961709018055E-4 48-49 0.0 0.0 0.0 0.06338437034015235 2.7845961709018055E-4 50-51 0.0 0.0 0.0 0.0700325936981804 2.7845961709018055E-4 52-53 0.0 0.0 0.0 0.08016156226983573 4.176894256352708E-4 54-55 0.0 0.0 0.0 0.10097641864732672 4.176894256352708E-4 56-57 0.0 0.0 0.0 0.13101524984092994 4.176894256352708E-4 58-59 0.0 0.0 0.0 0.16648404356779167 4.176894256352708E-4 60-61 0.0 0.0 0.0 0.21002816619026865 4.5249687777154337E-4 62-63 0.0 0.0 0.0 0.2519363385623409 4.8730432990781595E-4 64-65 0.0 0.0 0.0 0.2983346722599922 5.569192341803611E-4 66-67 0.0 0.0 0.0 0.36763630946331083 5.569192341803611E-4 68-69 0.0 0.0 0.0 0.44779787173314656 5.569192341803611E-4 70-71 0.0 0.0 0.0 0.48887066525394823 5.569192341803611E-4 72-73 0.0 0.0 0.0 0.5503754331787418 6.265341384529062E-4 74-75 0.0 0.0 0.0 0.6153261388650264 6.265341384529062E-4 76-77 0.0 0.0 0.0 0.6835139375999844 6.265341384529062E-4 78-79 0.0 0.0 0.0 0.7671562450834474 6.265341384529062E-4 80-81 0.0 0.0 0.0 0.8905486629065336 6.265341384529062E-4 82-83 0.0 0.0 0.0 1.0249750430568183 6.265341384529062E-4 84-85 0.0 0.0 0.0 1.2281809486283777 6.265341384529062E-4 86-87 0.0 0.0 0.0 1.5230000682226061 6.265341384529062E-4 88-89 0.0 0.0 0.0 1.8466745656378047 6.265341384529062E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTAGC 700 0.0 25.384584 1 TACCTGG 1330 0.0 25.35441 2 GTACCTG 1385 0.0 25.312757 1 GTATAGA 410 0.0 24.598114 1 GTAAGGT 550 0.0 24.178371 4 TACATCG 140 1.7832885E-5 23.744135 5 GTACATG 5985 0.0 23.591173 1 TACATGG 6265 0.0 22.288101 2 TATCACG 150 3.0364186E-5 22.164278 2 GAGTACT 5810 0.0 21.986057 12-13 ACATGGG 5970 0.0 21.797564 3 GTACTTT 5975 0.0 21.021273 14-15 TAAGGTG 635 0.0 20.939709 5 GGGCGTA 450 0.0 20.050604 9 AGGTAAG 660 0.0 19.429724 2 AGTACTT 6005 0.0 19.374224 12-13 GTACACG 200 1.1688733E-5 19.209955 1 GTAGGAC 375 9.094947E-12 18.997292 3 ATCACGC 150 7.7297474E-4 18.997292 3 CATGGGG 3070 0.0 18.873531 4 >>END_MODULE