FastQCFastQC Report
Thu 26 May 2016
SRR936621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482173
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48090.32445605202631544No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41000.27662088028860327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40200.27122339969760617No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30410.20517173096527866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22610.1525462952030566No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19850.13392498716411647No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC18170.12259027792302249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG13950.032.704482
GTACCTG15600.031.0721421
ATAACGC1401.6983904E-830.5509513
CGTATAG700.00837332327.1554831
GTACATG59600.023.9205191
GAGTACT49250.023.59218812-13
TACATGG61500.022.7186472
ACATGGG61050.022.0298233
GTACTTT52400.021.99257514-15
TAACGCA1302.9314868E-421.9347574
GTATCAA108150.021.0476951
GTGTAAG6850.020.1188431
GGTATCA84900.020.0387631
AGTACTT52950.020.01402712-13
ACCTGGG23200.019.8698233
AGAGTAC84700.019.27123610-11
TCAACGC123000.018.5464614
CAACGCA123200.018.5157285
ATCAACG123750.018.3950333
TATCAAC124200.018.290122
Produced by FastQC (version 0.11.5)